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1.
Biochem Biophys Res Commun ; 522(3): 743-748, 2020 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-31791581

RESUMO

Rheumatoid arthritis is a chronic and systemic autoimmune disease, which affects approximately 1% of the adult population worldwide. The present study investigated the therapeutic effect of theacrine (TC) on arthritis and its mechanisms in Freund's incomplete adjuvant (FIA)-induced SD rats. Rats were randomly divided into 5 groups: i) healthy control; ii) model; iii) positive control with methotrexate (MTX); iv) treatment with 12.5 mg/kg TC; and v) treatment with 25.0 mg/kg TC. The apparent scores, including changes in body weights, degree of paw swelling and arthritis indicators, were analyzed to evaluate the anti-chronic inflammatory effect of TC. The levels of interleukin (IL)-6 and transforming growth factor-ß (TGF-ß) in serum were measured by enzyme-linked immunosorbent assay. The protein and RNA expression levels of the critical factors in rats were measured to elucidate the mechanisms responsible for chronic inflammation and to verify molecular indexes of chronic inflammatory conditions. TC notably suppressed the severity of FIA-induced rat by attenuating the apparent scores, animal weight and inflammatory indexes in the 25 mg/kg TC group compared with the FIA rat model. Furthermore, TC significantly decreased the levels of IL-6 and increased the levels of TGF-ß. Histopathological examinations indicated that TC rescued the synovial hyperplasia and inflammatory cell infiltration in joint tissues. In addition, TC enhanced TGF-ß-mediated shifts in inflammatory marker expression in joint tissue. Overall, the present study demonstrated that TC exerted a superior anti-arthritic effect via the suppression of IL-6 and the activation of TGF-ß by the TGF-ß/SMAD pathway.

2.
Front Genet ; 10: 99, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30838028

RESUMO

Identifying the hallmarks of cancer is essential for cancer research, and the genes involved in cancer hallmarks are likely to be cancer drivers. However, there is no appropriate method in the current literature for identifying genetic cancer hallmarks, especially considering the interrelationships among the genes. Here, we hypothesized that "dense clusters" (or "communities") in the gene co-expression networks of cancer patients may represent functional units regarding cancer formation and progression, and the communities present in the co-expression networks of multiple types of cancer may be cancer hallmarks. Consequently, we mined the conserved communities in the gene co-expression networks of seven cancers in order to identify candidate hallmarks. Functional annotation of the communities showed that they were mainly related to immune response, the cell cycle and the biological processes that maintain basic cellular functions. Survival analysis using the genes involved in the conserved communities verified that two of these hallmarks could predict the survival risks of cancer patients in multiple types of cancer. Furthermore, the genes involved in these hallmarks, one of which was related to the cell cycle, could be useful in screening for cancer drugs.

3.
Zhongguo Zhong Yao Za Zhi ; 43(21): 4361-4364, 2018 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-30583642

RESUMO

The original plant species of Fructus Aurantii are multitudinous and complex, and their requirements to the growing environment is strict. In order to clarify the original plant species and geographical distribution of Fructus Aurantii which recorded in the standards and circulated, used in commodity. The national and local standards of Chinese medicinal materials were collected and the original plants of Fructus Aurantii recoded in standards were found. Ten original plant species of Fructus Aurantii (including varieties of cultivars, the same below) were recorded in the Chinese pharmacopoeia and six local standards of Zhejiang, Yunnan and Guizhou etc. The producing areas and commodity in markets of Fructus Aurantii were investigated. The growth environment and geographical distribution of them were analyzed. There are six types of Fructus Aurantii i.e., Fructus Aurantii Chuan, Fructus Aurantii Xiang, Fructus Aurantii Jiang, Fructus Aurantii Qu, Fructus Aurantii Su, Fructus Aurantii Wen, and nineteen species of original plants in the practical commodities. There are four major Fructus Aurantii producing areas: Sichuan Basin, Dongting Lake Plain, Poyang Lake Plain, Jinqu Basin and its surrounding hilly areas. All of them are located in the area of the east longitude 104° to 121° and the northern latitudes 27° to 31°. There is a certain difference between the actual commodity and the standards of medicinal materials. It is suggested that the traditional mainstream types of Fructus Aurantii with fine quality should be accepted into Chinese Pharmacopoeia, and the types with poor quality should be withdrawn from Chinese Pharmacopoeia.


Assuntos
Citrus/classificação , Citrus/crescimento & desenvolvimento , China , Medicamentos de Ervas Chinesas , Frutas , Geografia
4.
Zhongguo Zhong Yao Za Zhi ; 39(1): 153-6, 2014 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-24754186

RESUMO

General regulations for the processing are the important part of processing procedures of prepared slices of Chinese crude drugs. It has an important significance on enhancing the operability of actual production, regulating production of prepared slices of Chinese crude drugs, improving quality and establishing drug safety. The article could provides suggestions and reference for future compilation work on "National processing procedures of prepared slices of Chinese crude drugs" by comparative analysis and summary on general regulations for the processing of different processing procedures of prepared slices of Chinese crude drugs in China.


Assuntos
Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/normas , Medicina Tradicional Chinesa/normas , Tecnologia Farmacêutica/normas , China , Humanos , Controle de Qualidade , Segurança
5.
Chem Pharm Bull (Tokyo) ; 56(11): 1592-5, 2008 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-18981611

RESUMO

Simultaneous determination of polydatin and its metabolite in excrement samples using high-performance liquid chromatography (HPLC) with UV detection was accomplished. After extracted them by C18 solid phase extraction, the samples were separated on a reversed-phase column. Detection wave-lengths were set at 306 nm. The separation was carried out with a gradient elution. The mobile phase was acetonitrile-water (containing 0.1% formic acid) at a flow rate of 1.0 ml.min(-1). The identities of the peaks were accomplished by comparing retention times, UV and mass data with reference compounds under the same conditions. The standard curve was rectilinear in the range of 0.803-642.6 microg x ml(-1) (r=1.0000) for polydatin, 0.407-325.8 microg x ml(-1) (r=1.0000) for resveratrol. The recoveries of the markers listed above were 102.2% and 97.3%, respectively. The verified method can be used to determine the contents of two compounds in samples of stomach, small intestine, caecum, and large intestine (including excrement) of rats fed with polydatin. The analytical results demonstrated that the metabolism of polydatin is mainly processed in the intestines; polydatin can be transformed into resveratrol by de-sugaring process.


Assuntos
Medicamentos de Ervas Chinesas/análise , Fezes/química , Glucosídeos/análise , Estilbenos/análise , Animais , Calibragem , Cromatografia Líquida de Alta Pressão , Trato Gastrointestinal/química , Trato Gastrointestinal/metabolismo , Indicadores e Reagentes , Espectrometria de Massas , Ratos , Ratos Wistar , Reprodutibilidade dos Testes , Resveratrol , Espectrofotometria Ultravioleta
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