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1.
Chemistry ; 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31674074

RESUMO

The search for structurally simple chromophores with superior fluorescence brightness and wide range of solvent compatibility is highly desirable. Herein, we present a new type of single benzene-based solvatochromic chromophore ( 5 ) with a symmetric bifunctional structure, where the azetidine and ethoxy-carbonyl serve as the electron donating and electron withdrawing groups, respectively. This chromophore exhibits an extraordinary wide range of solvent compatibility and preserves excellent fluorescence quantum yields from non-polar n -hexane to polar methanol and even in water. Unusually, the symmetric structure 5 shows a distinct color change from bright green to red along increasing solvent polarity and possesses large Stokes shifts (132-207 nm) in the tested solvents. Moreover, this single benzene-based chromophore displays good photochemical stability in both solution and solid state, and even exhibits reversible mechanochromic luminescence.

2.
Anal Chem ; 2019 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-31621298

RESUMO

Film-based fluorescence sensing is recognized as one of the most optimized techniques for trace analysis of chemicals in the air after the invention of ion mobility spectrometry. The performance of the technique is highly dependent on the design of the film. This paper reports a new fluorescent film which shows unprecedented and discriminative sensing performance to the presence of phenol, o-cresol, m-cresol, and p-cresol in the air with an ultralow detection limit as low as 0.4, 0.3, 10, and 0.8 ppt, respectively. The film was designed via combination of the advantages of aggregation-induced emission (AIE) and those of intramolecular charge transfer (ICT), where the former provides the opportunity to avoid the widely encountered aggregation-caused quenching (ACQ) effect and the latter allows sensitive sensing of the microenvironment change of the film. The biggest challenge of the design is to find a fluorophore possessing both AIE and ICT effects. Fortunately, a newly synthesized biphenyl derivative of o-carborane capped with azetidine moiety (BZPCarb) shows the properties as expected. Importantly, the fluorophore is photochemically stable, a prerequirement for multiple uses of a film device. In addition, the nonplanar structure of the fluorophore is also favorable for film sensing as it could form porous films owing to screening of dense stacking of the molecules. It is the merits that make BZPCarb-based film show outstanding sensing and discriminative performances. Based on the fluorophore and the design, a conceptual high-performance fluorescent vapor sensor for phenolic compounds was developed.

3.
J Pharm Biomed Anal ; 176: 112819, 2019 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-31454661

RESUMO

Fupo Ganmao Granules (FP) is a classic traditional Chinese medicine (TCM) formula, composed of 4 herbal medicines, Folium Hibisci Mutabilis, Magnolia officinalis, Pericarpium Citri Reticulatae, and Fructus arctii. It is regularly used in the treatment of common cold with wind-heat syndrome. The biologically active constituents in FP, especially those in trace amount, remain unclear. In this study, the components of FP were profiled and characterized using HPLC-ESI-MS2. A total of 58 compounds were identified according to their fragmentation features in IT-TOF-MS and IT-MS2, including 13 phenolic acids, 17 flavonoids, 21 lignans, 2 alkaloids, and 5 other compounds. Among them, the identities of 8 compounds were explicitly confirmed by comparing them with standard compounds, and their contents were determined. Our study provided a comprehensive understanding of the diverse chemical profile in FP, and the approach we developed is useful for the analysis of other TCM formulas.

4.
Microbiome ; 7(1): 116, 2019 Aug 22.
Artigo em Inglês | MEDLINE | ID: mdl-31439031

RESUMO

BACKGROUND: Inflammasomes have been found to interact with the gut microbiota, and this effect is associated with depression, but the mechanisms underlying this interaction have not been elucidated in detail. RESULTS: The locomotor activity of NLRP3 KO mice was significantly greater than that of their WT littermates, while cohousing and transplantation of the NLRP3 KO gut microbiota avoid the effects of NLRP3 KO on the general locomotor activity at baseline. Meanwhile, transplantation of the NLRP3 KO microbiota alleviated the CUS-induced depressive-like behaviors. The compositions of the gut microbiota in NLRP3 KO mice and WT mice were significantly different in terms of the relative abundance of Firmicutes, Proteobacteria, and Bacteroidetes. Fecal microbiota transplantation (FMT) from NLRP3 KO mice significantly ameliorated the depressive-like behavior induced by chronic unpredictable stress (CUS) in recipient mice. Given the correlation between circular RNA HIPK2 (circHIPK2) and depression and the observation that the level of circHIPK2 expression was significantly increased in CUS-treated mice compared with that in the control group, further experiments were performed. FMT significantly ameliorated astrocyte dysfunction in recipient mice treated with CUS via inhibition of circHIPK2 expression. CONCLUSIONS: Our study illustrates the involvement of the gut microbiota-circHIPK2-astrocyte axis in depression, providing translational evidence that transplantation of the gut microbiota from NLRP3 KO mice may serve as a novel therapeutic strategy for depression.

5.
Chem Asian J ; 14(15): 2751-2758, 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31210030

RESUMO

A reliable and sensitive detection of biogenic amines (BAs) is essential to ensure food safety and maintain public health. In this study, two naphthyl end-capped terthiophene derivatives, namely, 5-(naphthalen-1-yl)-2,2':5',2''-terthiophene (NA-3T) and 5,5''-di(naphthalen-1-yl)-2,2':5',2''-terthiophene (NA-3T-NA), were employed to develop chemiresistive sensors for detecting gaseous BAs. In contrast to NA-3T, the NA-3T-NA-based sensor showed a higher sensitivity for trimethylamine (TMA) with an experimental detection limit lower than 22 ppm, and for aromatic BAs, including dopamine, histamine, tryptamine, and tyramine. Additionally, the recovery time for TMA was found to be shorter than 23 s. In addition, both sensors were successfully used for an in situ evaluation of meat freshness by monitoring the concentration of relevant volatile BAs. The difference in the sensing performances of the two chemiresistive sensors was tentatively ascribed to different packing structures of the derivatives and the adlayer structures of the films developed with the compounds.


Assuntos
Aminas Biogênicas/análise , Inocuidade dos Alimentos/métodos , Carne/análise , Tiofenos/química , Cristalografia por Raios X , Dopamina/análise , Conformação Molecular , Espectrofotometria , Temperatura Ambiente , Termogravimetria
6.
J Ethnopharmacol ; 242: 112029, 2019 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-31216433

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: ShengMai-Yin and Ganmaidazao decoction are classic formulas in traditional Chinese medicine. Individually, Shengmai-Yin is used to treat cardiovascular diseases, and Ganmaidazao decoction for therapy of mental disorders. The combination of Shengmai-Yin and Ganmaidazao decoction (SGD) is normally used as adjuvant therapy for type 2 diabetes mellitus (T2DM). AIM OF THE STUDY: The central aim is to elucidate the pharmacological efficacy of SGD and its mechanism in the treatment of T2DM with non-alcoholic fatty liver disease (NAFLD). MATERIALS AND METHODS: Active ingredients in SGD and their drug targets were identified using network analysis followed by experimental validation. First, existing databases were mined for information relevant to SGD, including pharmacological actions, chemical components, physicochemical characteristics, potential targets, and implicated diseases. Candidate patterns obtained with the network analysis were then tested in a KKAy mouse model of T2DM with NAFLD. Various doses of SGD were administered, followed by measurements of fasting blood glucose, oral glucose tolerance tests, insulin tolerance tests, markers of lipid metabolism - including free fatty acids (FFA), triglycerides (TG), and total cholesterol (TC) - liver histology, and expression levels of implicated molecules including PI3K/AKT and PPARα. RESULTS: Over 300 potential active compounds with their physicochemical characteristics and 562 candidate targets were collected, and then the network of them was constructed. Follow-up pathway and functional enrichment analyses indicated that SGD influences metabolism-related signaling pathways including PI3K-Akt, AMPK, and PPAR. In validation experiments, treatment of KKAy mice with SGD reduced serum levels of glucose, TC, TG, and FFA, decreased numbers of crown-like structures in visceral adipose tissue, reduced adipocyte size, and lowered liver lipid deposits. Further, SGD improved liver metabolism by increasing the expressions of PPARα, HSL, and PI3K/Akt, and decreasing expressions of SREBP-1 and FASN, inhibiting lipid biosynthesis, and increasing insulin sensitivity. CONCLUSION: Experimental validation of network analysis revealed anti-diabetic effects of the plant product SGD, manifested most notably by improved serum profiles and diminished insulin resistance. These experimental results may have clinical implications.

7.
J Leukoc Biol ; 106(5): 1089-1100, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31211478

RESUMO

Ginsenoside Rh2 (G-Rh2) has well-established potent antitumor activity; yet, the effects of G-Rh2 on immune and metabolism regulation in cancer treatment, especially non-small cell lung cancer (NSCLC) remain unclear. We showed that G-Rh2 had a synergistic antitumor effect with cyclophosphamide (CY) on mice with NSCLC, and improved the immune deficiency caused by CY. Consistently, G-Rh2 exhibited no inhibitory effect on tumor growth of T cells-deficient nude mice. Furthermore, G-Rh2 treatment triggered the oxidative decomposition of fatty acid (FA), suppressed FA synthesis, increased ketone level, and decreased glucocorticoid (CORT) secretion. G-Rh2 significantly down-regulated the expression of fatty acid synthase (FASN). Of note, in liver-specific FASN knockout mice G-Rh2 failed to show the same immune enhancement effects. Further mechanistic exploration revealed that G-Rh2 suppressed the expression and nuclear translocation of sterol regulatory element binding protein 1 (SREBP-1), and disturbed the SREBP-1-FASN interaction in vitro.

8.
Small ; 15(19): e1900735, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30963720

RESUMO

Emerging evidence indicates that exosomes derived from gastric cancer cells enhance tumor migration and invasion through the modulation of the tumor microenvironment. However, it remains a major problem to detect cancer-specific exosomes due to technical and biological challenges. Most of the methods reported could not achieve efficient detection of tumor-derived exosomes in the background of normal exosomes. Herein, a label-free electrochemical aptasensor is presented for specific detection of gastric cancer exosomes. This platform contains an anti-CD63 antibody modified gold electrode and a gastric cancer exosome specific aptamer. The aptamer is linked to a primer sequence that is complementary to a G-quadruplex circular template. The presence of target exosomes could trigger rolling circle amplification and produce multiple G-quadruplex units. This horseradish peroxidase mimicking DNAzyme could catalyze the reduction of H2 O2 and generate electrochemical signals. This aptasensor exhibits high selectivity and sensitivity toward gastric cancer exosomes with a detection limit of 9.54 × 102 mL-1 and a linear response range from 4.8 × 103 to 4.8 × 106 exosomes per milliliter. Therefore, this electrochemical aptasensor is expected to become a useful tool for the early diagnosis of gastric cancer.

9.
J Biomed Nanotechnol ; 15(5): 1090-1096, 2019 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-30890238

RESUMO

Procedures for enrichment, isolation and purification of exosomes from complex biological samples are difficult, tedious, non-standardized, and require bulky instrumentation such as ultracentrifugation (UC). In this article, a simple method for isolating exosomes. Size-Selective Method (SSM) was established based on commercially available materials, and the UC and ExoQuick-TC kits (EQkit) methods were compared in terms of morphology, particle size, quantity, Western Blot (WB), and extraction time. Results showed that all three different exosome separation methods could obtain circular membranous vesicles, with a diameter of 30-110 nm. There were more non-exosome components in the samples extracted by SSM, such as large microvesicles, with a lower purity. UC obtained a large number of exosomes with a higher purity, but it required an ultracentrifuge, costed much time and had low yield. Both EQkit and SSM were easy to operate, but EQkit tended to aggregate exosomes and consume much time. WB results showed that exosomes extracted by all the three methods expressed CD63 protein. The SSM had the highest CD63 protein content and at the same protein concentration. The above evidences showed that SSM was fast and had high recovery, low cost and high protein concentration, but had more non-exosome protein components, which can be a choice for exosome separation.


Assuntos
Exossomos , Western Blotting , Proteínas , Ultracentrifugação
10.
Dermatol Ther ; 32(3): e12862, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-30758895

RESUMO

Syphilis is a chronic systemic infectious disease involving multiple organs. Involvement of skeletal system by syphilis including osteochondritis, osteoarthritis, periostitis, and osteitis. Charcot arthropathy is an unusual concomitant disease of syphilis, and the incidence had decreased significantly these decades. The present authors report a case of syphilis presenting with Charcot arthropathy of bilateral ankle joints.

11.
Mol Oncol ; 13(4): 873-893, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-30628173

RESUMO

Forkhead box transcription factor M1 (FOXM1) is a proliferation-associated transcription factor involved in tumorigenesis through transcriptional regulation of its target genes in various cells, including dendritic cells (DCs). Although previous work has shown that FOXM1 enhances DC maturation in response to house dust mite allergens, it is not known whether FOXM1 affects DC maturation in the context of tumor-specific immunity. In this study, we examined the central role of FOXM1 in regulating bone marrow-derived dendritic cell (BMDC) maturation phenotypes and function in pancreatic cancer and colon cancer. FOXM1 retarded maturation phenotypes of BMDCs, inhibited promotion of T-cell proliferation, and decreased interleukin-12 (IL-12) p70 in tumor-bearing mice (TBM). Notably, FOXM1 expression was epigenetically regulated by dimethylation on H3 lysine 79 (H3K79me2), a modification present in both tumor cells and BMDCs. Increased H3K79me2 enrichment was observed at the FOXM1 promoter in both BMDCs from TBM, and in BMDCs from wild-type mice cultured with tumor-conditioned medium that mimics the tumor microenvironment (TME). Furthermore, inhibition of the H3K79 methyltransferase DOT1L not only decreased enrichment of H3K79me2, but also downregulated expression of FOXM1 and partially reversed its immunosuppressive effects on BMDCs. Furthermore, we found that FOXM1 upregulated transcription of Wnt family number 5A (Wnt5a) in BMDCs in vitro; we also observed that exogenous Wnt5a expression abrogated BMDC maturation phenotypes by inhibiting FOXM1 and H3K79me2 modification. Therefore, our results reveal that upregulation of FOXM1 by H3K79me2 in pancreatic cancer and colon cancer significantly inhibits maturation phenotypes and function of BMDCs through the Wnt5a signaling pathway, and thus provide novel insights into FOXM1-based antitumor immunotherapy.

12.
Anal Chem ; 2018 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-30398856

RESUMO

In situ, on line, noncontact, and fast monitoring of the compositions of ethanol-water mixtures via vapor-phase sampling has remained a challenge for years. In this work, we report for the first time a film-based fluorescent sensor showing unprecedented ability to discriminate the compositions of ethanol-water mixtures. Importantly, ethanol contents in the mixtures can vary from 0 to 100% (v/v), the response time is less than 2 s, and the sensing is fully reversible. More importantly, the monitoring was performed via vapor-phase sampling, avoiding sample contamination. The principle behind it is ascribed to the big difference in the fluorescent quantum yield of the sensing unit, a newly designed and synthesized monosubstituted fluorescent o-carborane derivative (ZPCarb), in the two solvents. In addition, the sensor as developed was successfully used for the determination of ethanol contents in four commercial liquors, suggesting its potential application in the quality control of beverages, in monitoring fermentation processes, and in other processes.

13.
Neuropsychiatr Dis Treat ; 14: 2783-2791, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30425496

RESUMO

Purpose: This prospective, single-arm, open-label, 8-week, multicenter study investigated the effectiveness of switching from immediate-release methylphenidate (IR-MPH) to osmotic controlled-release methylphenidate (OROS-MPH) in patients with attention-deficit/hyperactivity disorder (ADHD). Patients and methods: Overall, 296 patients with ADHD (mean age: 9.5 years) already on IR-MPH treatment were enrolled. Upon enrollment, a flexible dose of OROS-MPH was administered, replacing IR-MPH. Patients were assessed at baseline and weeks 2, 4, and 8 using the Swanson, Nolan, and Pelham version IV scale (SNAP-IV) and the Clinical Global Impression for ADHD symptoms. The Social Adjustment Inventory for Children and Adolescents assessed social functions, and the Chinese Health Questionnaire (CHQ) and Family Adaptation, Partnership, Growth, Affection, and Resolve evaluated parental and family functions. Results: Switching from IR-MPH to OROS-MPH yielded significant improvements in all ADHD symptoms, as rated by parents, teachers (SNAP-IV), and study investigators (Clinical Global Impression). CHQ scores and all Social Adjustment Inventory for Children and Adolescents subscores except spare time scores improved significantly. Patients with poor IR-MPH adherence had greater improvements in teacher-rated SNAP-IV and mothers' mental health (CHQ) after switching. Conclusion: Switching from IR-MPH to OROS-MPH improved patients' behavioral ADHD symptoms and social adjustment, and mental health of patients' mothers. This was most evident in patients who previously exhibited poor IR-MPH adherence.

14.
Mol Psychiatry ; 2018 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-30413800

RESUMO

Circular RNAs (circRNAs), highly expressed in the central nervous system, are involved in various regulatory processes and implicated in some pathophysiology. However, the potential role of circRNAs in psychiatric diseases, particularly major depressive disorder (MDD), remains largely unknown. Here, we demonstrated that circular RNA DYM (circDYM) levels were significantly decreased both in the peripheral blood of patients with MDD and in the two depressive-like mouse models: the chronic unpredictable stress (CUS) and lipopolysaccharide (LPS) models. Restoration of circDYM expression significantly attenuated depressive-like behavior and inhibited microglial activation induced by CUS or LPS treatment. Further examination indicated that circDYM functions as an endogenous microRNA-9 (miR-9) sponge to inhibit miR-9 activity, which results in a downstream increase of target-HECT domain E3 ubiquitin protein ligase 1 (HECTD1) expression, an increase of HSP90 ubiquitination, and a consequent decrease of microglial activation. Taken together, the results of our study demonstrate the involvement of circDYM and its coupling mechanism in depression, providing translational evidence that circDYM may be a novel therapeutic target for depression.

15.
Clin Chem ; 64(12): 1772-1779, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30201803

RESUMO

BACKGROUND: The glycoproteinoses are a subgroup of lysosomal storage diseases (LSDs) resulting from impaired degradation of N-linked oligosaccharide side chains of glycoproteins, which are commonly screened by detecting the accumulated free oligosaccharides (FOSs) in urine via thin layer chromatography (TLC). The traditional TLC method suffers from limited analytical sensitivity and specificity and lacks quantification capability. Therefore, we developed an analytically sensitive and relatively specific assay using ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for urinary FOS analysis and validated its use for urine screening of glycoproteinoses and other LSDs. METHODS: Urine volumes equivalent to 30 µg of creatinine were derivatized with butyl-4-aminobenzoate and then purified through a solid-phase extraction cartridge. A 7-min UPLC-MS/MS analysis was performed on a triple quadrupole mass spectrometer using an amide column for separation of derivatized FOS. Urine samples from >100 unaffected controls and 37 patients with various LSDs were studied. RESULTS: Relative quantification was conducted on 7 selected FOSs using a single internal standard, which allowed the identification of patients with 1 of 8 different LSDs: aspartylglucosaminuria, α-fucosidosis, α-mannosidosis, ß-mannosidosis, ß-galactosidase deficiency, Sandhoff disease, sialidosis, and galactosialidosis. Patients treated with hematopoietic stem cell transplant show decreased FOS responses compared with untreated patients. CONCLUSIONS: This UPLC-MS/MS assay offers a valuable tool for screening of glycoproteinoses and other LSDs, with potential use for future treatment monitoring.

16.
J Neuroinflammation ; 15(1): 221, 2018 Aug 04.
Artigo em Inglês | MEDLINE | ID: mdl-30075790

RESUMO

BACKGROUND: Mice with pilocarpine-induced temporal lobe epilepsy (TLE) are characterized by intense hippocampal neuroinflammation, a prominent pathological hallmark of TLE that is known to contribute to neuronal hyperexcitability. Recent studies indicate that Adam10, a member of a disintegrin and metalloproteinase domain-containing protein (Adam) family, has been involved in the neuroinflammation response. However, it remains unclear whether and how Adam10 modulates neuroinflammation responses in the context of an epileptic brain or whether Adam10 affects epileptogenesis via the neuroinflammation pathway. METHODS: Adult male C57BL/6J mice were subjected to intraperitoneal injection of pilocarpine to induce TLE. Adeno-associated viral (AAV) vectors carrying Adam10 (AAV-Adam10) or lentiviral vectors carrying short hairpin RNA, which is specific to the mouse Adam10 mRNA (shRNA-Adam10), were bilaterally injected into the hippocampus to induce overexpression or knockdown of Adam10, respectively. The specific anti-inflammatory agent minocycline was administered following status epilepticus (SE) to block hippocampal neuroinflammation. Continuous video EEG recording was performed to analyze epileptic behavior. Western blot, immunofluorescence staining, and ELISA were performed to determine Adam10 expression as well as hippocampal neuroinflammation. RESULTS: In this study, we demonstrate that overexpression of Adam10 in the hippocampus suppresses neuroinflammation and reduces seizure activity in TLE mice, whereas knockdown of Adam10 exacerbates hippocampal neuroinflammation and increases seizure activity. Furthermore, increased seizure activity in Adam10 knockdown TLE mice is dependent on hippocampal neuroinflammation. CONCLUSION: These results suggest that Adam10 suppresses epilepsy through repression of hippocampal neuroinflammation. Our findings provide new insights into the Adam10 regulation of development of epilepsy via the neuroinflammation pathway and identify a potential therapeutic target for epilepsy.

17.
Autophagy ; 14(7): 1164-1184, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29938598

RESUMO

Circular RNAs (circRNAs) are highly expressed in the central nervous system and are involved in the regulation of physiological and pathophysiological processes. However, the potential role of circRNAs in stroke remains largely unknown. Here, using a circRNA microarray, we showed that circular RNA Hectd1 (circHectd1) levels were significantly increased in ischemic brain tissues in transient middle cerebral artery occlusion (tMCAO) mouse stroke models and further validated this finding in plasma samples from acute ischemic stroke (AIS) patients. Knockdown of circHectd1 expression significantly decreased infarct areas, attenuated neuronal deficits, and ameliorated astrocyte activation in tMCAO mice. Mechanistically, circHECTD1 functions as an endogenous MIR142 (microRNA 142) sponge to inhibit MIR142 activity, resulting in the inhibition of TIPARP (TCDD inducible poly[ADP-ribose] polymerase) expression with subsequent inhibition of astrocyte activation via macroautophagy/autophagy. Taken together, the results of our study indicate that circHECTD1 and its coupling mechanism are involved in cerebral ischemia, thus providing translational evidence that circHECTD1 can serve as a novel biomarker of and therapeutic target for stroke. ABBREVIATIONS: 3-MA: 3-methyladenine; ACTB: actin beta; AIS: acute ischemic stroke; AS: primary mouse astrocytes; BECN1: beclin 1, autophagy related; BMI: body mass index; circHECTD1: circRNA HECTD1; circRNAs: circular RNAs; CBF: cerebral blood flow; Con: control; DAPI: 4',6-diamidino-2-phenylindole; ECA: external carotid artery; FISH: fluorescence in situ hybridization; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; Gdna: genomic DNA; GFAP: glial fibrillary acidic protein; GO: gene ontology; HDL: high-density lipoprotein; IOD: integrated optical density; LDL: low-density lipoprotein; LPA: lipoprotein(a); MAP1LC3B: microtubule-associated protein 1 light chain 3 beta; MIR142: microRNA 142; mNSS: modified neurological severity scores; MRI: magnetic resonance imaging; NIHSS: National Institute of Health Stoke Scale; OGD-R: oxygen glucose deprivation-reperfusion; PCR: polymerase chain reaction; PFA: paraformaldehyde; SQSTM1: sequestosome 1; TIPARP: TCDD inducible poly(ADP-ribose) polymerase; tMCAO: transient middle cerebral artery occlusion; TTC: 2,3,5-triphenyltetrazolium chloride; UTR: untranslated region; WT: wild type.

18.
Biosens Bioelectron ; 109: 27-34, 2018 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-29524914

RESUMO

DNA has emerged as a promising biomaterial for assembling a variety of nanostructures based on its programmable base pairing. It also has other remarkable properties including stability, prominent biocompatibility, and can easily be modified with functional groups for further applications. In the past few decades, researchers have established various design rules and assembly technologies to improve the stability and complexity of DNA nanostructures. The detection of cancer-associated biomarkers has significant importance in identifying patients with different clinical stages and also in developing adaptive therapeutic strategies. Due to their unique advantages, DNA nanostructures can be designed to serve as universal units to form biosensors for the detection of tumor biomarkers. In this review, we first present a brief introduction of the development of structural DNA nanotechnology. Then we summarize recent strategies for DNA nanostructure-based optical, electrochemical and mass sensitive biosensors in cancer detection. Finally, we discuss the challenges and opportunities these technologies provide.


Assuntos
Biomarcadores Tumorais/isolamento & purificação , Técnicas Biossensoriais , DNA/química , Neoplasias/diagnóstico , Biomarcadores Tumorais/genética , DNA/genética , Técnicas Eletroquímicas , Humanos , Nanoestruturas/química , Neoplasias/genética
19.
Aerosp Med Hum Perform ; 89(2): 141-146, 2018 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-29463359

RESUMO

INTRODUCTION: The objective of the study was to investigate the effects of dropping heights and prophylactic ankle braces on ankle joint biomechanics during half-squat parachute landing from two different heights. METHODS: There were 30 male elite paratroopers with formal parachute landing training and more than 2 yr of parachute jumping experience who were recruited for this study. The subjects tested three different ankle brace conditions (no-brace, elastic brace, semirigid brace). Each subject was instructed to jump off a platform from two different heights of 0.4 m and 0.8 m, and land on a force plate in a half-squat posture. The Vicon 3D motion capture system and force plate were used to record and calculate kinematic and kinetic data. RESULTS: Dropping height had a significant effect on peak vertical ground reaction force (vGRF), maximum ankle angular displacement, and time to vGRF. As compared with the no-brace group, use of an elastic ankle brace significantly reduced peak vGRF by 18.57% and both braces significantly reduced the maximal angular displacements of dorsiflexion. The semirigid brace provided greater restriction against maximal angular displacement of inversion. DISCUSSION: The elastic and semirigid ankle braces both effectively restricted motion stability of the ankle joint in the sagittal plane, and the semirigid ankle brace prevented excessive inversion, although the comfort of this device should be improved overall.Wu D, Zheng C, Wu J, Hu T, Huang R, Wang L, Fan Y. Prophylactic ankle braces and the kinematics and kinetics of half-squat parachute landing. Aerosp Med Hum Perform. 2018; 89(2):141-146.


Assuntos
Articulação do Tornozelo/fisiologia , Aviação , Braquetes , Militares , Adulto , Fenômenos Biomecânicos , China , Humanos , Masculino , Adulto Jovem
20.
Integr Cancer Ther ; 17(3): 860-866, 2018 09.
Artigo em Inglês | MEDLINE | ID: mdl-29355051

RESUMO

The aim of the present study was to investigate whether fraction from Lycium barbarum polysaccharide (LBP) could reduce immunotoxicity and enhance antitumor activity of doxorubicin (Dox) in mice. A water-soluble LBP fraction, designated LBP3, was isolated from edible Chinese herbal Lycium barbarum and used in this study. To investigate the effect of LBP3 on Dox-induced immunotoxicity, tumor-free mice were used and treated with either normal saline, Dox, or Dox plus LBP3. To investigate the effect of LBP3 on antitumor activity of Dox, H22 tumor-bearing mice were used and treated with either normal saline, Dox, LBP3, or Dox plus LBP3. The results showed that LBP3 did not protect against the body weight loss caused by Dox, but it promoted the recovery of body weight starting at day 5 after Dox treatment in tumor-free mice. LBP3 also improved peripheral blood lymphocyte counts, promoted cell cycle recovery in bone marrow cells, and restored the cytotoxicity of natural killer cells. Furthermore, in H22 tumor-bearing mice, LBP3 enhanced antitumor activity of Dox and improved peripheral blood lymphocyte counts and the cytotoxicity of splenocytes. In brief, our results demonstrated that LBP3 could reduce the immunotoxicity and enhance antitumor activity of Dox.

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