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1.
Clin Nucl Med ; 2022 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-35020667

RESUMO

ABSTRACT: A 35-year-old man with mesenteric metastases of unknown primary was referred for 18F-FDG PET/MRI. The images demonstrated that FDG accumulated in the chest, abdomen, bilateral kidneys, and external genitalia. Renal and testicular metastases were suspected. The primary tumor was still not found. In addition, kidney biopsy findings indicated a diagnosis of IgA nephropathy.

2.
Phytomedicine ; 94: 153843, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34785414

RESUMO

BACKGROUND: Obesity is the cause of multiple metabolic disorders, and its incidence has been rapidly increasing worldwide. It develops when energy intake exceeds energy expenditure (EE). Wedelolactone (WDL) is a naturally isolated compound from Eclipta prostrata L. and possesses many pharmacological activities. However, little is known about the effect of WDL on obesity and EE. PURPOSE: The present study aimed to investigate the effect of WDL on obesity and EE in diet-induced obese (DIO) mice and its underlying mechanism. METHODS: Obese mice were induced by high fat diet. The effects of WDL on obese mice were assessed by examining body weight, fat mass, EE, glucose tolerance, and hepatic and kidney injury. 3T3-L1 cells were differentiated into mature adipocytes and incubated with WDL in vitro. Immunohistochemistry, western blotting, and real-time PCR were used to assess adipose browning. The inhibitory efficiency of WDL on nicotinamide N-methyltransferase (NNMT) was evaluated using a fluorescence assay. RESULTS: WDL reduced fat mass, suppressed body weight gain, and improved obesity-related metabolic disorders in DIO mice. WDL treatment promoted adipose browning and enhanced EE in both DIO mice and 3T3-L1 cells. These effects were eliminated in AMPK antagonized or PPARα knockdown cells and in PPARα-/- mice. Furthermore, we identified the target of WDL to be NNMT, an appealing target for regulating energy metabolism. WDL inhibited NNMT with an extremely low IC50 of 0.03 µM. Inhibition of NNMT and activation of SIRT1/AMPK/PPARα explains how WDL reverses obesity by prompting adipose browning. CONCLUSION: Our findings demonstrate the novel effects of WDL in promoting adipose browning, enhancing EE and attenuating obesity and uncover the underlying mechanism, which includes inhibition of NNMT and subsequently activation of SIRT1/AMPK/PPARα in response to WDL. WDL could be further developed as a therapeutic agent for treating obesity and related metabolic diseases.


Assuntos
Nicotinamida N-Metiltransferase , Sirtuína 1 , Células 3T3-L1 , Proteínas Quinases Ativadas por AMP , Animais , Cumarínicos , Dieta Hiperlipídica , Camundongos , Camundongos Endogâmicos C57BL , Obesidade/tratamento farmacológico , PPAR alfa , Compostos Fitoquímicos
3.
J Med Virol ; 94(2): 659-666, 2022 02.
Artigo em Inglês | MEDLINE | ID: mdl-34499353

RESUMO

Serum hepatitis B core antibody (anti-HBc) is associated with liver inflammation in chronic hepatitis B patients. This study aimed to investigate whether anti-HBc could serve as a predictor of significant liver inflammation in hepatitis B e antigen (HBeAg)-negative chronic hepatitis B virus (HBV) infected patients with normal alanine aminotransferase (ALT) and detectable HBV DNA. Treatment-naïve HBeAg-negative chronic HBV infected patients with normal ALT and detectable HBV DNA who underwent liver biopsy were retrospectively included from two medical centers. Liver inflammation grade was evaluated using the Scheuer scoring system and significant liver inflammation was defined as ≥G2. Serum anti-HBc levels were measured by commercial immunoassays (Abbott GmbH & Co. KG). A total of 117 patients were included and 50 (42.7%) patients showed significant liver inflammation. Serum anti-HBc levels in patients with significant liver inflammation were significantly higher than patients with no or mild liver inflammation (

4.
J Colloid Interface Sci ; 607(Pt 2): 1589-1602, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-34587533

RESUMO

It is of great significance to understand the role of carrier in piezocatalysis of composites by studying the separation mode of carriers under dynamic polarization field. Herein, the separation and migration pathways of carriers under piezoelectric field are investigated by synthesizing heterojunctions with Bi2WO6 (BWO) nanosheets grown vertically on g-C3N4 (CN) coated ZnO nanorods and directly on ZnO. Compared with the photocatalysis, the piezocatalytic efficiency of Rhodamine B (RhB) by BWO/ZnO is significantly increased to 0.121 min-1, which indicated the polarization field promotes band tilt and Z-scheme formation. After introducing the CN interlayer, the piezocatalytic efficiency of BWO/CN/ZnO is further improved (0.217 min-1), which can be attributed to the unique core-shell structure with Z-scheme heterojunctions. This unique structure provides more active sites and excited carrier concentration, the intermediate layer CN also reduces the direct contact and recombination of electrons and holes controlled by polarization potential at the interface between BWO and ZnO. This work deeply analyzes the influence of carrier concentration, separation efficiency and transport process on piezocatalysis, which provides a reference for the design of efficient catalysts.


Assuntos
Poluentes Ambientais , Óxido de Zinco , Catálise , Luz
5.
Redox Biol ; 49: 102217, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-34942528

RESUMO

Maintaining host iron homeostasis is an essential component of nutritional immunity responsible for sequestrating iron from pathogens and controlling infection. Nucleotide-oligomerization domain-like receptors (NLRs) contribute to cytoplasmic sensing and antimicrobial response orchestration. However, it remains unknown whether and how NLRs may regulate host iron metabolism, an important component of nutritional immunity. Here, we demonstrated that NLRP6, a member of the NLR family, has an unconventional role in regulating host iron metabolism that perturbs host resistance to bacterial infection. NLRP6 deficiency is advantageous for maintaining cellular iron homeostasis in both macrophages and enterocytes through increasing the unique iron exporter ferroportin-mediated iron efflux in a nuclear factor erythroid-derived 2-related factor 2 (NRF2)-dependent manner. Additional studies uncovered a novel mechanism underlying NRF2 regulation and operating through NLRP6/AKT interaction and that causes a decrease in AKT phosphorylation, which in turn reduces NRF2 nuclear translocation. In the absence of NLRP6, increased AKT activation promotes NRF2/KEAP1 dissociation via increasing mTOR-mediated p62 phosphorylation and downregulates KEAP1 transcription by promoting FOXO3A phosphorylation. Together, our observations provide new insights into the mechanism of nutritional immunity by revealing a novel function of NLRP6 in regulating iron metabolism, and suggest NLRP6 as a therapeutic target for limiting bacterial iron acquisition.

6.
Clin Interv Aging ; 16: 1975-1986, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34876810

RESUMO

Accumulating evidence has demonstrated that gut microbial-derived metabolite trimethylamine N-oxide (TMAO) plays a crucial role in the pathogenesis of many diseases and can be served as a prognostic biomarker for several cardiovascular disorders, including arrhythmia. Recently, some studies have documented that TMAO was associated with the occurrence, progression, recurrence, and embolism risk of atrial fibrillation (AF). The activation of related inflammatory signal pathways and the cardiac sympathetic nervous system (CSNS) caused by elevated TAMO may be the underlying mechanism. It is worth noting that intervention in the metabolic pathway of TMAO may be an underlying therapeutic target of AF. In addition, standardized and individualized treatment strategies in clinical practice may be of great significance for AF patients, particularly those with high serum TMAO concentrations. However, there are also contradictions in the current research on TMAO and AF. Moreover, notwithstanding the positive preclinical and clinical findings, data supporting a direct association between TMAO and AF is a paucity. Thus, conclusive evidence from preclinical studies and multi-center randomized controlled trials to reveal the essential relationship between TMAO and AF is needy. In this review, we have attempted to summarize recent studies on TMAO and AF, highlighted the potential therapeutic strategies for AF patients, followed by a discussion on directions for future research in this field.

7.
Artigo em Inglês | MEDLINE | ID: mdl-34851087

RESUMO

Stimuli-responsive smart nanocarriers are an emerging class of materials applicable in fields including drug delivery and tissue engineering. Instead of constructing responsive polymer shells to control the release and delivery of drugs, in this work, we put forward a novel strategy to endow the internal drugs with light responsivity. The microcapsule consisted of molecular motor (MM)-doped cholesteric liquid crystals (CLCs) and drugs. The drug in gelatin-gum arabic microcapsules can protect the carried drugs for a long time with a low release speed totally resulting from drug diffusion. Under UV light, the MM isomerizes and the chirality changes, inducing the alteration of the superstructure of the CLCs. In this process, the cooperative molecular disturbance accelerates the diffusion of the drugs from the microcapsule core to the outside. As a result, thanks to the cooperative effect of liquid crystalline mesogens, molecular-scale geometric changes of motors could be amplified to the microscale disturbance of the self-organized superstructure of the CLCs, resulting in the acceleration of the drug release. This method is hoped to provide opportunities in the design and fabrication of novel functional drug delivery systems.

8.
Rev Esp Enferm Dig ; 2021 12 02.
Artigo em Inglês | MEDLINE | ID: mdl-34852632

RESUMO

We present the case of a 37-year-old male who admitted to our hospital with fever, weakness, limb pain for 6 days and dyspnea for 14 hours .The patient had no immune related diseases.He was rapidly diagnosed with fulminant myocarditis and progressed to severe cardiogenic shock during the early stage.Then he was treated with V-A extracorporeal membrane oxygenation (ECMO). It is worth mentioning that the patient's peripheral blood was taken for metagenomic Next-Generation Sequencing(mNGS) upon admission and the results did not find any pathogenic bacteria.But there is no further examination(such as coronary angiography and myocardial biopsy) to determine the etiology of myocarditis.

9.
Front Neurosci ; 15: 704603, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34867145

RESUMO

The human-robot interface (HRI) based on biological signals can realize the natural interaction between human and robot. It has been widely used in exoskeleton robots recently to help predict the wearer's movement. Surface electromyography (sEMG)-based HRI has mature applications on the exoskeleton. However, the sEMG signals of paraplegic patients' lower limbs are weak, which means that most HRI based on lower limb sEMG signals cannot be applied to the exoskeleton. Few studies have explored the possibility of using upper limb sEMG signals to predict lower limb movement. In addition, most HRIs do not consider the contribution and synergy of sEMG signal channels. This paper proposes a human-exoskeleton interface based on upper limb sEMG signals to predict lower limb movements of paraplegic patients. The interface constructs an channel synergy-based network (MCSNet) to extract the contribution and synergy of different feature channels. An sEMG data acquisition experiment is designed to verify the effectiveness of MCSNet. The experimental results show that our method has a good movement prediction performance in both within-subject and cross-subject situations, reaching an accuracy of 94.51 and 80.75%, respectively. Furthermore, feature visualization and model ablation analysis show that the features extracted by MCSNet are physiologically interpretable.

10.
Annu Int Conf IEEE Eng Med Biol Soc ; 2021: 1076-1081, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34891474

RESUMO

The human-robot interface (HRI) based on surface electromyography(sEMG) can realize the natural interaction between human and robot. It has been widely used in exoskeleton robots recently to help predict the wearer's movement. The sEMG signal of the paraplegic patients' lower limbs is weak. How to achieve accurate prediction of the lower limb movement of patients with paraplegia has always been the focus of attention in the field of HRI. Few studies have explored the possibility of using upper limb sEMG signals to predict lower limb movement. In addition, most HRIs do not consider the contribution and synergy of sEMG signal channels. This paper proposes a human-exoskeleton interface based on upper limb sEMG signals to predict lower limb movements of paraplegic patients. The interface constructs a channel synergy-based network (MCSNet) to extract the contribution and synergy of different feature channels. An sEMG data acquisition experiment is designed to verify the effectiveness of MCSNet. The experimental results show that our method has a good movement prediction performance in both within-subject and cross-subject situations, reaching an accuracy of 94.51% and 80.75% respectively.

11.
BMC Public Health ; 21(1): 2303, 2021 Dec 19.
Artigo em Inglês | MEDLINE | ID: mdl-34923971

RESUMO

BACKGROUND: The present study aimed to develop and test an extended theory of planned behavior (TPB), which includes attitudes, subjective norms and perceived behavioral control, oral health knowledge, and past oral health behavior on the intention to improve oral health behaviors among primary school students in Shanghai, China. METHODS: A school-based cross-sectional study was conducted with 414 students in the third-grade from 10 classes of Mingqiang Primary School located in Shanghai, China. Participants were recruited in October 2019. Data were collected through self-reported questionnaires, consisting of demographic characteristics, TPB variables, oral health knowledge and past oral health behaviors. Exploratory factor analysis was used to analyze TPB items. Pearson's correlation and hierarchical regression analyses were conducted to identify the associated factors of intention to improve oral health behaviors. RESULTS: The study showed that among students in the third grade, attitudes, subjective norms, perceived behavioral control, and past oral health behaviors were associated with the intention to improve oral health behaviors. In the hierarchical regression analysis, age and sex were entered in Model 1 which significantly explained 3.00% of the variance (F = 6.26, p < 0.01). The addition of Model 2 variables of attitudes, perceived behavioral control, subjective norms, and oral health knowledge revealed that TPB variables explained 26.70% (F = 29.59, p < 0.01). For Model 3, the addition of past oral health behaviors accounted for a further 1% of variance, and the full model has accounted for 28.30% of the variance with the intention to improve oral health behaviors (F = 22.8, p < 0.01). Regression analyses supported that among the significant variables, perceived behavioral control had the largest beta weight, followed by subjective norms and past oral health behaviors. CONCLUSION: The extended TPB model constructed in this study could be used to explain children's intentions to improve oral health behaviors. Children's oral health-related perceived behavioral control, subjective norms, and past oral health behaviors may serve as priority intervention targets in oral health promotion practices aimed at children.

12.
Front Oncol ; 11: 764258, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34912712

RESUMO

Background: Total thyroidectomy (TT) or lobectomy without radioactive iodine (RAI) is becoming a common management for patients with low-risk differentiated thyroid cancer (DTC). However, the assessment of response to therapy for these patients remains controversial. The aim of this study was to propose and validate a new dynamic evaluation strategy to assess the response to therapy in patients with low-risk DTC treated with TT or lobectomy but without RAI. Methods: We performed a retrospective analysis of 543 adult patients with low-risk DTC who underwent TT or lobectomy without RAI therapy. Follow-up consisted of trends of serum thyroglobulin (Tg), anti-thyroglobulin antibody (TgAb) levels and neck ultrasonography (US) were conducted every 6-24 months. Response to therapy assessments were defined as excellent response, biochemical incomplete response, structural incomplete response, and indeterminate response according to the follow-up findings. Results: At a median follow-up of 51 months (range 33-66 months), 517 (95%) had excellent response, while the other 26 had either biochemical incomplete response (an increasing trend of suppressed serum Tg levels, n=9; an increasing trend of TgAb levels, n=3) or indeterminate response (a stable or decreasing trend of suppressed serum Tg levels, but a stable positive trend of TgAb levels, n=14). No patients had structural incomplete response or no deaths related to thyroid cancer. The risk of incomplete response was significantly higher in lobectomy than in TT (p<0.001). Conclusion: Our study proposed and validated a new dynamic response to therapy assessment depending on trends of suppressed serum Tg, TgAb levels, and neck US findings which could be an appropriate tool for postoperative follow-up in low-risk DTC patients without RAI therapy. Our findings provided further evidence to support no routine recommendation of RAI after surgery in low-risk DTC.

13.
J Cyst Fibros ; 2021 Dec 24.
Artigo em Inglês | MEDLINE | ID: mdl-34961706

RESUMO

BACKGROUND: This study was performed to describe the natural history of CF lung disease in young children over an 18 month period to assess the use of CT scanning as an outcome measure for intervention trials. METHODS: Chest CT scans were obtained at baseline and after 18 months in 42 two- to six-year-old children with CF. CT scans were scored by 2 experienced radiologists for the presence and severity of bronchiectasis, mucous plugging, and air trapping. RESULTS: Mean age at baseline 3.5 (1.3) (mean, sd) years. One or more findings of CF lung disease was seen on the first CT in 27 (64%) and at 18 months in 30 (75%). From baseline to 18 months bronchiectasis, mucous plugging, and air trapping increased from 50% to 53%, 14% to 28%, and 48% to 58% respectively. There was marked variability in the rate of progression, with subjects commonly showing improvement in lung disease. Bronchiectasis worsened in 14 (33%) and improved in 13 (31%). Single subjects with F508del/class III and F508del/class V demonstrated greater worsening and improvement respectively than F508del homozygous and class I genotypes. CONCLUSIONS: The natural history of CF lung disease over 18 months varies widely between subjects. Factors including genotype may affect natural history as well as the effectiveness of mediators and could be an important confounder if not recognized. These findings suggest that the use of CT scanning as an outcome surrogate for CF lung disease in young children may be more challenging than has been previously recognized.

14.
Front Oncol ; 11: 774455, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34950584

RESUMO

Purpose: A combined model was established based on the MRI-radiomics of pre- and mid-treatment to assess the risk of disease progression or death in locally advanced nasopharyngeal carcinoma. Materials and Methods: A total of 243 patients were analyzed. We extracted 10,400 radiomics features from the primary nasopharyngeal tumors and largest metastatic lymph nodes on the axial contrast-enhanced T1 weighted and T2 weighted in pre- and mid-treatment MRI, respectively. We used the SMOTE algorithm, center and scale and box-cox, Pearson correlation coefficient, and LASSO regression to construct the pre- and mid-treatment MRI-radiomics prediction model, respectively, and the risk scores named P score and M score were calculated. Finally, univariate and multivariate analyses were used for P score, M score, and clinical data to build the combined model and grouped the patients into two risk levels, namely, high and low. Result: A combined model of pre- and mid-treatment MRI-radiomics successfully categorized patients into high- and low-risk groups. The log-rank test showed that the high- and low-risk groups had good prognostic performance in PFS (P<0.0001, HR: 19.71, 95% CI: 12.77-30.41), which was better than TNM stage (P=0.004, HR:1.913, 95% CI:1.250-2.926), and also had an excellent predictive effect in LRFS, DMFS, and OS. Conclusion: Risk grouping of LA-NPC using a combined model of pre- and mid-treatment MRI-radiomics can better predict disease progression or death.

15.
Hell J Nucl Med ; 24(3): 267-268, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34901967

RESUMO

A 51-year-old woman with papillary thyroid cancer (PTC) complained of headache.Physical examination found the tongue derivation torightside. Fluorine-18-fluorodeoxyglucose positron emission tomography/magnetic resonance imaging (18F-FDG PET/MRI) detected a hypermetabolic soft-tissue mass in the right retropharyngeal carotid space, where the nasopharyngeal carotid segment of right hypoglossal nerve passing inferiorly. The musculature in the right side of tongue was inflammatory instead of fatty infiltration, with hyperintense on T2-weighted image and hypermetabolism on PET. These images together indicated the early phase of hypoglossal nerve palsy caused by PTC invasion.

16.
Clin Lab ; 67(12)2021 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-34910444

RESUMO

BACKGROUND: Endogenous endophthalmitis (EE) is a severe sight-threatening ocular infection caused by a variety of microbes. Here, we report a rare case of Endogenous Klebsiella pneumoniae endophthalmitis (EKPE) related to Klebsiella pneumonia (Kp) from a catheter. METHODS: A 76-year-old man suffered bacteremia one month after a right internal jugular venous catheter was administered due to the need of parenteral nutrition after pancreatoduodenectomy. The results of catheter tip culture and blood culture indicated that he had extended-spectrum ß-lactamases (ESBL) (+) Kp. One month after recovery, the patient had a high fever that was accompanied by a sudden loss of vision in his left eye. ESBL (+) Kp was found in the vitreous fluid and blood. RESULTS: The patient was diagnosed with EKPE. The patient underwent three-port pars plana vitrectomy (PPV) in the left eye, biapenem was administered intravenously for four weeks. One week after the withdrawal of the biapenem, the patient's right neck was palpated with a strip-shaped mass of about 10.0 cm x 5.0 cm. Incision and drainage of the right neck abscess was performed. ESBL (+) Kp was found in the abscess of the neck cultures. Imipenem/cilastatin and ciprofloxacin were administered post-operation and gradually changed to ceftazidime. Amoxicillin/clavulanate potassium and levofloxacin were administered to the patient after being discharged from hospital. During the follow-up, the patient recovered from the bacteremia but still had no light perception in his left eye. CONCLUSIONS: A patient with a Kp infection and blurred vision should have ocular examination especially for patients with diabetes.


Assuntos
Endoftalmite , Infecções por Klebsiella , Idoso , Antibacterianos/uso terapêutico , Cateteres , Endoftalmite/diagnóstico , Endoftalmite/tratamento farmacológico , Humanos , Infecções por Klebsiella/diagnóstico , Infecções por Klebsiella/tratamento farmacológico , Klebsiella pneumoniae , Masculino
17.
Small Methods ; 5(11): e2100787, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34927961

RESUMO

Dielectric film capacitors are considered as potential candidates for advanced power electronics technology due to their extremely high-power densities and outstanding mechanical and thermal stability, but the further improvement of energy storage density is still needed. Here, a strategy is proposed to enhance the energy storage properties by introducing nanoscale polarization regions into amorphous films, which can significantly improve the maximum polarization and maintain a high breakdown strength. The (1-x)BaTiO3 -xBi(Ni0.5 Zr0.5 )O3 ((1-x)BT-xBNZ) thin films are fabricated by the sol-gel method and the amorphous films with nanoscale polarization regions are obtained by adjusting the preparation process. Consistent with the conjecture, amorphous phase and nanoscale polarization regions in the (1-x)BT-xBNZ films are clearly observed by electron diffraction. Results show that giant recoverable energy density of 103.7 J cm-3 with high energy efficiency of 88.3% are simultaneously achieved at 8.3 MV cm-1 in 0.92BT-0.08BNZ thin films. Furthermore, the 0.92BT-0.08BNZ thin film exhibits excellent thermal stability in a wide temperature range of 20-200 °C, ∆Wrec /Wrec20 °C  < 2.2%. This work provides a novel method for dielectric thin film capacitors applied in high temperature and electric field.

18.
Front Aging Neurosci ; 13: 761248, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34899273

RESUMO

Background: Perivascular adipose tissue (PVAT) imaging can be used in clinical practice as a surrogate marker of vascular disease. We aimed to analyze the association between the density of carotid artery PVAT and clinical features and outcomes in stroke patients treated with mechanical thrombectomy. Methods: A total of 183 consecutive patients treated with mechanical thrombectomy due to anterior circulation large vessel occlusion were retrospectively included from January 2016 to May 2021. The density of carotid artery PVAT was evaluated by preoperative computed tomography angiography. Successful arterial recanalization was defined as a modified Thrombolysis in Cerebral Infarction score of 2b-3 on the final angiographic examination. Poor functional outcome was defined as a modified Rankin Scale (mRS) score > 2 at 3 months after stroke. We assessed the independent effect of carotid artery PVAT density on revascularization, functional outcome, and mortality using logistic regression models adjusted for relevant confounders. Results: Patients with large artery atherosclerotic stroke have higher carotid artery PVAT density than patients with other stroke etiologies (-65.82 ± 12.96 vs. -75.77 ± 13.44, P < 0.001). Higher carotid artery PVAT density was associated with unsuccessful recanalization [adjusted odds ratio (AOR) (95% CI), 2.968 (1.292, 6.819), P = 0.010], and poor outcome [AOR (95% CI), 2.704 (1.610, 4.541), P < 0.001] and mortality [AOR (95% CI), 1.894 (1.040, 3.449), P = 0.037] at 3 months in stroke patients treated with thrombectomy. Conclusion: Higher carotid artery PVAT density before mechanical thrombectomy is an indicator of worse postprocedural arterial revascularization and a worse functional outcome in acute stroke patients.

19.
Acta Pharm Sin B ; 11(11): 3481-3492, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34900531

RESUMO

Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related deaths, characterized by highly hypoxic tumor microenvironment. Hypoxia-inducible factor-1α (HIF-1α) is a major regulator involved in cellular response to changes of oxygen levels, supporting the adaptation of tumor cells to hypoxia. Bruceine D (BD) is an isolated natural quassinoid with multiple anti-cancer effects. Here, we identified BD could significantly inhibit the HIF-1α expression and its subsequently mediated HCC cell metabolism. Using biophysical proteomics approaches, we identified inhibitor of ß-catenin and T-cell factor (ICAT) as the functional target of BD. By targeting ICAT, BD disrupted the interaction of ß-catenin and ICAT, and promoted ß-catenin degradation, which in turn induced the decrease of HIF-1α expression. Furthermore, BD could inhibit HCC cells proliferation and tumor growth in vivo, and knockdown of ICAT substantially increased resistance to BD treatment in vitro. Our data highlight the potential of BD as a modulator of ß-catenin/HIF-1α axis mediated HCC metabolism.

20.
World J Surg Oncol ; 19(1): 348, 2021 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-34930333

RESUMO

OBJECTIVE: Immediate reconstruction (IR) is a safe and effective surgical treatment for patients with breast cancer. We aimed to assess the prognosis, aesthetic outcomes, and patient satisfaction of IR compared with breast conservation surgery (BCS) and total mastectomy (TM). METHODS: This retrospective matched-cohort study was conducted between May 2005 and December 2014. We established two cohorts according to the tumor (T) size of breast cancer. In the T≤3cm group, cases (IR) and controls (BCS or TM) were matched for age, pathological tumor size, and pathologic nodal status in a 1:1:1 ratio. In the T>3cm group, cases (IR) and controls (TM) were matched with the same factors and ratio. The primary outcome was the 5-year disease-free survival (DFS). The secondary outcome was patient satisfaction and quality of life. RESULTS: A total of 12,678 breast cancer patients were assessed for eligibility, of which 587 were included (T≤3 cm group: 155 IR vs 155 BCS vs 155 TM; T>3cm group: 61 IR vs 61 TM). In the T≤3 cm cohort, patients who underwent IR had no difference compared with those who underwent BCS or TM regarding the 5-year DFS (P=0.539); however, an improved aesthetic satisfaction, psychosocial, and sexual well-being were achieved in the IR group (P<0.001). In the T>3 cm cohort, the IR group had a worse median 5-year DFS (P=0.044), especially for Her2+ or triple-negative breast carcinoma (TNBC) subtypes compared with the TM group. CONCLUSIONS: IR improves aesthetic satisfaction, psychosocial, and sexual well-being for breast cancer patients with T≤3 cm. For patients with T > 3 cm invasive breast cancer, TM is superior to IR as it predicts a better 5-year DFS.


Assuntos
Neoplasias da Mama , Procedimentos Cirúrgicos Reconstrutivos , Neoplasias da Mama/cirurgia , Estudos de Coortes , Feminino , Humanos , Mastectomia , Qualidade de Vida , Estudos Retrospectivos
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