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1.
Sci Adv ; 6(35): eaba7457, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32923627

RESUMO

Breakdown of the blood-retinal barrier (BRB) causes retinal edema and vision loss. We investigated the role of Wnt signaling in maintaining the BRB by limiting transcytosis. Mice lacking either the Wnt co-receptor low-density lipoprotein receptor-related protein 5 (Lrp5-/- ) or the Wnt ligand Norrin (Ndpy/- ) exhibit increased retinal vascular leakage and enhanced endothelial transcytosis. Wnt signaling directly controls the transcription of an endothelium-specific transcytosis inhibitor, major facilitator superfamily domain-containing protein 2a (MFSD2A), in a ß-catenin-dependent manner. MFSD2A overexpression reverses Wnt deficiency-induced transcytosis in endothelial cells and in retinas. Moreover, Wnt signaling mediates MFSD2A-dependent vascular endothelium transcytosis through a caveolin-1 (CAV-1)-positive caveolae pathway. In addition, levels of omega-3 fatty acids are also decreased in Wnt signaling-deficient retinas, reflecting the basic function of MFSD2A as a lipid transporter. Our findings uncovered the Wnt/ß-catenin/MFSD2A/CAV-1 axis as a key pathway governing endothelium transcytosis and inner BRB integrity.

2.
Signal Transduct Target Ther ; 5(1): 181, 2020 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-32879300

RESUMO

Growing evidences suggest that the fibroblast growth factor/FGF receptor (FGF/FGFR) signaling has crucial roles in a multitude of processes during embryonic development and adult homeostasis by regulating cellular lineage commitment, differentiation, proliferation, and apoptosis of various types of cells. In this review, we provide a comprehensive overview of the current understanding of FGF signaling and its roles in organ development, injury repair, and the pathophysiology of spectrum of diseases, which is a consequence of FGF signaling dysregulation, including cancers and chronic kidney disease (CKD). In this context, the agonists and antagonists for FGF-FGFRs might have therapeutic benefits in multiple systems.

3.
Nutrients ; 12(8)2020 Jul 31.
Artigo em Inglês | MEDLINE | ID: mdl-32751784

RESUMO

Lactobacillus reuteri, a commensal intestinal bacteria, has various health benefits including the regulation of immunity and intestinal microbiota. We examined whether L. reuteri I5007 could protect mice against colitis in ameliorating inflammation, modulating microbiota, and metabolic composition. In vitro, HT-29 cells were cultured with L. reuteri I5007 or lipopolysaccharide treatment under three different conditions, i.e., pre-, co- (simultaneous), and posttreatment. Pretreatment with L. reuteri I5007 effectively relieves inflammation in HT-29 cells challenged with lipopolysaccharide. In vivo, mice were given L. reuteri I5007 by gavage throughout the study, starting one week prior to dextran sulfate sodium (DSS) treatment for one week followed by two days without DSS. L. reuteri I5007 improved DSS-induced colitis, which was confirmed by reduced weight loss, colon length shortening, and histopathological damage, restored the mucus layer, as well as reduced pro-inflammatory cytokines levels. Analysis of 16S rDNA sequences and metabolome demonstrates that L. reuteri I5007 significantly alters colonic microbiota and metabolic structural and functional composition. Overall, the results demonstrate that L. reuteri I5007 pretreatment could effectively alleviate intestinal inflammation by regulating immune responses and altering the composition of gut microbiota structure and function, as well as improving metabolic disorders in mice with colitis.

4.
Neural Netw ; 131: 154-162, 2020 Aug 06.
Artigo em Inglês | MEDLINE | ID: mdl-32781384

RESUMO

Deep learning based on deep neural networks of various structures and architectures has been powerful in many practical applications, but it lacks enough theoretical verifications. In this paper, we consider a family of deep convolutional neural networks applied to approximate functions on the unit sphere Sd-1 of Rd. Our analysis presents rates of uniform approximation when the approximated function lies in the Sobolev space W∞r(Sd-1) with r>0 or takes an additive ridge form. Our work verifies theoretically the modelling and approximation ability of deep convolutional neural networks followed by downsampling and one fully connected layer or two. The key idea of our spherical analysis is to use the inner product form of the reproducing kernels of the spaces of spherical harmonics and then to apply convolutional factorizations of filters to realize the generated linear features.

5.
Zhen Ci Yan Jiu ; 45(6): 431-7, 2020 Jun 25.
Artigo em Chinês | MEDLINE | ID: mdl-32643878

RESUMO

OBJECTIVE: To investigate the effect of electroacupuncture (EA) at "Baihui "(GV20) and "Shenshu "(BL23) on activation of glial cells, expression of inflammatory factor proteins and aquaporin 4 (AQP4)in the hippocampus of amyloid precursor protein/presenilin-1 (APP/PS1) transgenic mice, so as to explore its mechanisms underlying improvement of Alzheimer's disease(AD). METHODS: Twenty C57/BL6 background male APP695/PS1-dE9(APP/PS1) double transgenic mice (model group) and 20 wild type (WT) C57/BL6 mice (blank group) were respectively randomized into control and EA groups. EA (2 Hz/15 Hz, 1-2 mA) was applied to GV20 and bilateral BL23 for 30 min, once daily, 6 days a week for 4 weeks. The recognition memory ability was detected by novel object recognition tests in a behavior test box. The percentage of time spent in close interaction with novel object (C) relative to the total time was used to generate preference index. The contents of hippocampal ß amyloid protein (Aß)1-40 and Aß1-42 were assayed using ELISA, and the expression levels of glial fibrillary acidic protein (GFAP), ionic calcium binding receptor molecule-1 (Iba-1), interleukin-1ß (IL-1ß), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) proteins in the hippocampus measured by Western blot. The activities of hippocampal astrocytes (GFAP-labelled cells), microglia (Iba-1-labelled cells) and the polarity expression of AQP4 (for removing Aß) were measured by immunohistochemistry. RESULTS: The preference index was significantly decreased in the model group relatively to the blank control group (P<0.05) and considerably increased in the model+EA group relatively to the model group (P<0.05), suggesting an improvement of the recognition memory after EA. The contents of Aß1-40 and Aß1-42, immunoactivity of GFAP and Iba-1, expression levels of GFAP, Iba-1, IL-1ß, IL-6 and TNF-α proteins were significantly higher in the model group than in the blank control group (P<0.01,P<0.05), while the AQP4 immunoactivity was notably lower in the model group than in the blank control group (P<0.05). Compared with the model group, the levels of Aß1-40 and Aß1-42, GFAP, Iba-1, IL-1ß, IL-6 and TNF-α proteins, and the percentage of Aß plaque area were significantly decreased in the model+EA group (P<0.01,P<0.05), and the immunoactivity of AQP4 was significantly increased in the mo-del+EA group (P<0.05). No significant changes were found in the above-mentioned indexes in the blank+EA group relevant to the blank control group (P>0.05).. CONCLUSION: EA at GV20 and BL23 can reduce inflammatory reaction and Aß level, suppress activation of astrocytes and microglia, and up-regulate expression of AQP4 in the hippocampus tissue in APP/PS1 transgenic mice, which may contribute to its effect in improving recognition memory ability, suggesting a role of EA intervention in delaying the development of AD via promoting the drainage of Aß by the glymphatic system in the brain.


Assuntos
Doença de Alzheimer , Eletroacupuntura , Precursor de Proteína beta-Amiloide , Animais , Aquaporina 4 , Modelos Animais de Doenças , Hipocampo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Presenilina-1
6.
Artigo em Inglês | MEDLINE | ID: mdl-32628934

RESUMO

PURPOSE: The aim of this study was to evaluate the effect of porous layer thickness in a 3-dimensionally printed 1-piece molar porous root-analogue implant (RAI) on the biomechanical properties of the peri-implant bone and the clinical efficacy of one such implant in a patient. MATERIALS AND METHODS: Three RAIs with different superficial porous layer thicknesses (0.5 mm, 1 mm, and fully porous) were designed and assembled using a mandible model and then solidified to obtain 3 finite elements models, denoted A, B, and C. Finite element analysis was performed to analyze the stress on the solid and porous structures of the RAIs and the stress and strain experienced by the bone surrounding the implant. RAIs were fabricated by selective laser melting. An unrepairable molar in a single patient was selected for replacement. An RAI was designed and prepared and then implanted into the alveolar bone immediately after minimally invasive extraction of the damaged tooth. Definitive restorations were placed after a 3-month period of uninterrupted healing. RESULTS: The stress concentration observed in the 3 types of RAI was principally between the solid and porous interface contact points, with maximum stress on the solid and porous structures smaller than that of the respective yield strength. The introduction of a porous structure on the surface of the RAIs increased peri-implant bone stress, which increased with thickness of the porous layer. The 3-dimensionally printed porous RAI exhibited excellent initial stability immediately after implantation. After continual observation for 6 months, it was found that bone surrounding the root had infiltrated into the RAI, achieving good osseointegration. CONCLUSIONS: Stress shielding can be reduced by decreasing the elastic modulus of the implant, with the interface between implant and bone allowing more appropriate stress conduction. A 1-piece porous RAI fabricated using 3-dimensional printing establishes a new indication for immediate implantation after extraction.

7.
Plant Biotechnol J ; 2020 Jul 17.
Artigo em Inglês | MEDLINE | ID: mdl-32677132

RESUMO

The incorporation of resistance genes into wheat commercial varieties is the ideal strategy to combat stripe or yellow rust (YR). In a search for novel resistance genes, we performed a large-scale genomic association analysis with high-density 660K single nucleotide polymorphism (SNP) arrays to determine the genetic components of YR resistance in 411 spring wheat lines. Following quality control, 371 972 SNPs were screened, covering over 50% of the high-confidence annotated gene space. Nineteen stable genomic regions harbouring 292 significant SNPs were associated with adult-plant YR resistance across nine environments. Of these, 14 SNPs were localized in the proximity of known loci widely used in breeding. Obvious candidate SNP variants were identified in certain confidence intervals, such as the cloned gene Yr18 and the major locus on chromosome 2BL, despite a large extent of linkage disequilibrium. The number of causal SNP variants was refined using an independent validation panel and consideration of the estimated functional importance of each nucleotide polymorphism. Interestingly, four natural polymorphisms causing amino acid changes in the gene TraesCS2B01G513100 that encodes a serine/threonine protein kinase (STPK) were significantly involved in YR responses. Gene expression and mutation analysis confirmed that STPK played an important role in YR resistance. PCR markers were developed to identify the favourable TraesCS2B01G513100 haplotype for marker-assisted breeding. These results demonstrate that high-resolution SNP-based GWAS enables the rapid identification of putative resistance genes and can be used to improve the efficiency of marker-assisted selection in wheat disease resistance breeding.

8.
Cell Rep ; 31(12): 107812, 2020 Jun 23.
Artigo em Inglês | MEDLINE | ID: mdl-32579938

RESUMO

The medial prefrontal cortex (mPFC) is a brain region involved in the affective components of pain and undergoes plasticity during the development of chronic pain. Dopamine (DA) is a key neuromodulator in the mesocortical circuit and modulates working memory and aversion. Although DA inputs into the mPFC are known to modulate plasticity, whether and how these inputs affect pain remains incompletely understood. By using optogenetics, we find that phasic activation of DA inputs from the ventral tegmental area (VTA) into the mPFC reduce mechanical hypersensitivity during neuropathic pain states. Mice with neuropathic pain exhibit a preference for contexts paired with photostimulation of DA terminals in the mPFC. Fiber photometry-based calcium imaging reveals that DA increases the activity of mPFC neurons projecting to the ventrolateral periaqueductal gray (vlPAG). Together, our findings indicate an important role of mPFC DA signaling in pain modulation.

9.
Artigo em Inglês | MEDLINE | ID: mdl-32543738

RESUMO

Smart self-protection is essential for addressing safety issues of energy-storage devices. However, conventional strategies based on sol-gel transition electrolytes often suffer from unstable self-recovery performance. Herein, smart separators based on thermal-gated poly(N-isopropylacrylamide) (PNIPAM) hydrogel electrolytes were developed for rechargeable zinc-ion batteries (ZIBs). Such PNIPAM-based separators not only display a pore structure evolution from opened to closed states, but also exhibit a surface wettability transition from hydrophilic to hydrophobic behaviors when the temperature rises. This behavior can suppress the migration of electrolyte ions across the separators, realizing the self-protection of ZIBs at high temperatures. Furthermore, the thermal-gated behavior is highly reversible, even after multiple heating/cooling cycles, because of the reversibility of temperature-dependent structural evolution and hydrophilic/hydrophobic transition. This work will pave the way for designing thermal-responsive energy-storage devices with safe and controlled energy delivery.

10.
World J Gastroenterol ; 26(18): 2126-2137, 2020 May 14.
Artigo em Inglês | MEDLINE | ID: mdl-32476780

RESUMO

Hepatocellular carcinoma (HCC) is the most common primary liver cancer with a dismal prognosis, especially when diagnosed at advanced stages. Annexin A2 (ANXA2), is found to promote cancer progression and therapeutic resistance. However, the underlining mechanisms of ANXA2 in immune escape of HCC remain poorly understood up to now. Herein, we summarized the molecular function of ANXA2 in HCC and its relationship with prognosis. Furthermore, we tentatively elucidated the underlying mechanism of ANXA2 immune escape of HCC by upregulating the proportion of regulatory T cells and the expression of several inhibitory molecules, and by downregulating the proportion of natural killer cells and dendritic cells and the expression of several inhibitory molecules or effector molecules. We expect a lot of in-depth studies to further reveal the underlying mechanism of ANXA2 in immune escape of HCC in the future.

11.
Sci Total Environ ; 735: 139456, 2020 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-32470669

RESUMO

Even though the relationship between population density and air pollution in China has been studied in recent years, most previous studies have suggested that an increase in population density increases air pollution. Using a panel dataset of 284 cities over the years 2003-2016 and 30 provinces during 2004-2015, as well as PM2.5 and SO2 used as air pollution indicators, the results show that an increase in population density will reduce air pollution in china. Through the mediation effect test, we found that clean energy and public transportation are the two mediation channels for population agglomeration to affect air quality. The concentration of population in cities is conducive to reducing the average cost of natural monopoly industries such as electricity, coal gas, liquefied petroleum gas, natural gas, and public transportation, thereby increasing residents' consumption of clean energy and public transportation services, reducing gas emissions that cause pollution, and improving air quality.

12.
Mikrochim Acta ; 187(6): 322, 2020 05 12.
Artigo em Inglês | MEDLINE | ID: mdl-32394285

RESUMO

Nitrogen-doped graphene quantum dots (N-GQDs) were synthesized by direct electrolysis of a carbon cloth electrode coated with nitrogen-doped nanomesh graphene (NG) in high yield (~ 25%). The N-GQDs emit intense blue fluorescence with a quantum yield (QY) of 10% ± 3%. Meanwhile, the N-GQDs are rich in hydroxyl, carboxyl, basic pyridinic nitrogen, and nitro groups, which are conducive to strengthen the interaction between N-GQDs and Fe3+ for highly sensitive determination of Fe3+ ions. Specifically, the determination for Fe3+ was conducted at different concentrations of N-GQD solution with a wide linear range of 10-1000 µM (150 µg·mL-1) and a low detection limit of 0.19 µM (10 µg·mL-1). Moreover, the fluorescence quenching mechanism illustrated that the functional groups generated by electrochemical oxidation enhanced the interaction of N-GQDs and Fe3+, and the narrow band gap (2.83 eV) of N-GQDs accomplished electron transfer from N-GQDs to Fe3+ easily. Graphical abstract A highly conductive carbon cloth electrode coated with nitrogen-doped nanomesh graphene (NG) was developed to prepared nitrogen-doped graphene quantum dots (N-GQDs) which was endowed with a wide linear range from 10 to 1000 µM (150 µg/mL) and a low detection limit of 0.19 µM (10 µg/mL) in the determination of Fe3+.

13.
Cell Host Microbe ; 28(2): 313-321.e6, 2020 08 12.
Artigo em Inglês | MEDLINE | ID: mdl-32470328

RESUMO

Selective and targeted removal of individual species or strains of bacteria from complex communities can be desirable over traditional, broadly acting antibacterials in several contexts. However, generalizable strategies that accomplish this with high specificity have been slow to emerge. Here we develop programmed inhibitor cells (PICs) that direct the potent antibacterial activity of the type VI secretion system (T6SS) against specified target cells. The PICs express surface-displayed nanobodies that mediate antigen-specific cell-cell adhesion to effectively overcome the barrier to T6SS activity in fluid conditions. We demonstrate the capacity of PICs to efficiently deplete low-abundance target bacteria without significant collateral damage to complex microbial communities. The only known requirements for PIC targeting are a Gram-negative cell envelope and a unique cell surface antigen; therefore, this approach should be generalizable to a wide array of bacteria and find application in medical, research, and environmental settings.

14.
ACS Appl Mater Interfaces ; 12(24): 26926-26935, 2020 Jun 17.
Artigo em Inglês | MEDLINE | ID: mdl-32432849

RESUMO

Electrophysiological measurement of molecular translocation through a nanopore is the fundamental basis of nanopore sensing. Free translocation of nucleic acids however is normally so fast that the identities of the compounds are not clearly resolvable. Inspired by recent progress in fluorescence imaging based nanopore sensing, we found that during electrophysiology measurements, translocation of nucleic acids is also retarded whenever a calcium flux around the pore vicinity is established. The residence time of nucleic acids has been extended to tens of milliseconds, a result of the strong coupling between nucleic acids and free calcium ions. The methodology presented here is applicable to both DNAs and RNAs and is able to clearly discriminate between different RNA homopolymers. This offers new insights for calcium imaging based nanopore sensing and suggests a new strategy of electrophysiology-based nanopore sensing aimed at a retarded motion of nucleic acids.

15.
ACS Nano ; 2020 May 27.
Artigo em Inglês | MEDLINE | ID: mdl-32432458

RESUMO

Among various energy storage devices, aqueous zinc-ion batteries (ZIBs) have captured great attention due to their high safety and low cost. One of the most promising cathodes of aqueous ZIBs is layered vanadium-based compounds. However, they often suffer from the capacity decaying during cycling. Herein, we prepared KV3O8·0.75H2O (KVO) and further incorporated it into a single-walled carbon nanotube (SWCNT) network, achieving freestanding KVO/SWCNT composite films. The KVO/SWCNT cathodes exhibit a Zn2+/H+ insertion/extraction mechanism, resulting in fast kinetics of ion transfer. In addition, the KVO/SWCNT composite films possess a segregated network structure, which offers the fast kinetics of electron transfer and guarantees an intimate contact between KVO and SWCNTs during cycling. As a result, the resultant batteries deliver a high capacity of 379 mAh g-1, excellent rate capability, and an ultralong cycle life up to 10,000 cycles with a high capacity retention of 91%. In addition, owing to the high conductivity and flexibility of KVO/SWCNT films, flexible soft-packaged ZIBs based on KVO/SWCNT film cathodes were assembled and displayed stable electrochemical performance at different bending states.

16.
Angew Chem Int Ed Engl ; 59(29): 11800-11807, 2020 Jul 13.
Artigo em Inglês | MEDLINE | ID: mdl-32301196

RESUMO

The reversible capacity of AlCl4 - intercalation/de-intercalation in conventional cathodes of aluminum-ion batteries (AIBs) is difficult to improve due to the large size of AlCl4 - anions. Therefore, it is highly desirable to realize the intercalation/de-intercalation of smaller Al-based ions. Here, we fabricated polyaniline/single-walled carbon nanotubes (PANI/SWCNTs) composite films and protonated the PANI nanorods. The protonation endows PANI with more active sites and enhanced conductivity. Hyper self-protonated PANI (PANI(H+ )) exhibits reversible AlCl2 + intercalation/de-intercalation during the discharge/charge process. As a result, the discharge capacity of the Al/PANI(H+ ) battery is twice as high as that of the initial composite films. PANI(H+ )@SWCNT electrodes also have a stable cycling life with only 0.003 % capacity decay per cycle over 8000 cycles. Owing to the excellent mechanical properties, PANI(H+ )@SWCNT composite films can act as the electrodes of flexible AIBs.

17.
Plant Dis ; 104(6): 1751-1762, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32293995

RESUMO

Stripe rust caused by Puccinia striiformis f. sp. tritici (Pst) is one of the most destructive fungal diseases of wheat worldwide. The expanding Yr26-virulent Pst race (V26) group overcomes almost all currently deployed resistance genes in China and has continued to accumulate new virulence. Investigating the genetic architecture of stripe rust resistance in common wheat is an important basis for a successful utilization of resistance in breeding programs. A panel of 410 exotic wheat germplasms was used for characterizing new stripe rust resistance loci. This panel was genotyped using high-density wheat 660K single-nucleotide polymorphism (SNP) array, and phenotypic evaluation of seedlings for stripe rust resistance was performed using multiple Pst races. Thirty-five loci conferring resistance were identified through genome-wide association mapping, and explained phenotypic variances ranged from 53 to 75%. Of these, 14 were colocated in the proximity of the known loci, including cataloged Yr genes Yr9, Yr10, Yr26, Yr33, Yr47, Yr56, Yr57, Yr64, Yr67, Yr72, and Yr81 and three temporarily designated as YrCen, YrNP63, and YrRC detected in our quantitative trait locus (QTL) mapping studies. Seven of them (Yr9, Yr10, Yr24/26, Yr81, YrCEN, YrNP63, and YrRC) were confirmed by molecular detection or genetic analysis. New loci that were identified to be different from reported Yr genes need further confirmation. Nine QTL with significantly large phenotypic effect on resistance to all tested races were considered as major loci for effective resistance. The identified loci enrich our stripe rust resistance gene pool, and the linked SNPs should be useful for marker-assisted selection in breeding programs.


Assuntos
Resistência à Doença , Triticum , China , Estudo de Associação Genômica Ampla , Humanos , Desequilíbrio de Ligação , Doenças das Plantas , Locos de Características Quantitativas
18.
Angiogenesis ; 23(3): 385-394, 2020 08.
Artigo em Inglês | MEDLINE | ID: mdl-32140799

RESUMO

To examine whether free fatty acid receptor 4 (FFAR4) activation can protect against choroidal neovascularization (CNV), which is a common cause of blindness, and to elucidate the mechanism underlying the inhibition, we used the mouse model of laser-induced CNV to mimic angiogenic aspects of age-related macular degeneration (AMD). Laser-induced CNV was compared between groups treated with an FFAR4 agonist or vehicle, and between FFAR4 wild-type (Ffar4+/+) and knock out (Ffar4-/-) mice on a C57BL/6J/6N background. The ex vivo choroid-sprouting assay, including primary retinal pigment epithelium (RPE) and choroid, without retina was used to investigate whether FFAR4 affects choroidal angiogenesis. Western blotting for pNF-ĸB/NF-ĸB and qRT-PCR for Il-6, Il-1ß, Tnf-α, Vegf, and Nf-ĸb were used to examine the influence of FFAR4 on inflammation, known to influence CNV. RPE isolated from Ffar4+/+ and Ffar4-/- mice were used to assess RPE contribution to inflammation. The FFAR4 agonist suppressed laser-induced CNV in C57BL/6J mice, and CNV increased in Ffar4-/- compared to Ffar4+/+ mice. We showed that the FFAR4 agonist acted through the FFAR4 receptor. The FFAR4 agonist suppressed mRNA expression of inflammation markers (Il-6, Il-1ß) via the NF-ĸB pathway in the retina, choroid, RPE complex. The FFAR4 agonist suppressed neovascularization in the choroid-sprouting ex vivo assay and FFAR4 deficiency exacerbated sprouting. Inflammation markers were increased in primary RPE cells of Ffar4-/- mice compared with Ffar4+/+ RPE. In this mouse model, the FFAR4 agonist suppressed CNV, suggesting FFAR4 to be a new molecular target to reduce pathological angiogenesis in CNV.

19.
Mikrochim Acta ; 187(4): 218, 2020 03 12.
Artigo em Inglês | MEDLINE | ID: mdl-32166530

RESUMO

A nonenzymatic voltammetric assay for dopamine (DA) was developed based on the combination of three-dimensional graphene (3D Gr) and indium oxide nanosheet arrays (In2O3 NSAs). 3D Gr was prepared by chemical vapor deposition (CVD), and In2O3 NSAs were grown on its surface by hydrothermal synthesis. The results show that 3D Gr maintains a good porous structure (200 µm), and the pore size of In2O3 NSAs is 0.50 µm. Differential pulse voltammetry (DPV) is mainly used to determine the electrochemical properties of In2O3 NSAs/3D Gr. It possesses a sensitivity of 2.69 µA·µM-1·cm-2 towards DA (5-60 µM) at 0.14 V, and the detection limit (LOD) is 0.10 µM (S/N = 3). The recoveries obtained for spiked samples in the real sample detection is 105 (± 8)%. Graphical abstractSchematic representation of DA sensitive detection by growing In2O3 nanosheets arrays on three-dimentional graphene modified ITO.

20.
iScience ; 23(3): 100916, 2020 Feb 14.
Artigo em Inglês | MEDLINE | ID: mdl-32113156

RESUMO

MicroRNAs (miRNAs) are a class of short non-coding RNAs that function in RNA silencing and post-transcriptional gene regulation. However, direct characterization of miRNA is challenging due to its unique properties such as its low abundance, sequence similarities, and short length. Although urgently needed, single molecule sequencing of miRNA has never been demonstrated, to the best of our knowledge. Nanopore-induced phase-shift sequencing (NIPSS), which is a variant form of nanopore sequencing, could directly sequence any short analytes including miRNA. In practice, NIPSS clearly discriminates between different identities, isoforms, and epigenetic variants of model miRNA sequences. This work thus demonstrates direct sequencing of miRNA, which serves as a complement to existing miRNA sensing routines by the introduction of the single molecule resolution. Future engineering of this technique may assist miRNA-based early stage diagnosis or inspire novel cancer therapeutics.

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