Metabolic characteristics of ginsenosides from Panax ginseng in rat feces mediated by gut microbiota.

Ginsenosides in Panax ginseng are regarded to be functional ingredients for diverse pharmacological effects and orally administrated with very low absorption in the gastrointestinal tract to be metabolized by gut microbiota. However, in vivo metabolic characteristics of ginsenosides mediated by gut microbiota are not well-known. This study aimed to explore the metabolic profiles of ginsenosides in rat feces mediated by gut microbiota. Ginsenosides and metabolites were identified and relatively quantified by ultra-performance liquid chromatography tandem/quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS/MS). As a result, eighty-four metabolites were identified in the normal control rat feces, while only thirty intermediates were found with very low yields in the pseudo-germ-free (GF) group. Similarly, the main bioconversion pathways of ginsenosides in vivo were the same deglycosylation reaction mediated by gut microbiota in vitro. The findings demonstrated significant differences in metabolic profiles between the normal control and pseudo-GF rats, which implied gut microbiota played an important role in the metabolism of ginsenosides.

Microplastic pollution and ecological risk assessment of Yueqing Bay affected by intensive human activities.

Microplastics (MPs) are a widespread environmental problem posing ecological risks in the ocean. We investigated the abundance, spatial distribution, characteristics and ecological risks of MPs in surface seawater, sediments and organisms in Yueqing Bay, China. MPs were detected in both environmental media and organisms. The overall abundance (0.24 items/m3 seawater, 6.13 items/kg dry sediment, 0.77 items/individual in organisms) was low to medium compared with other coastal areas. The MPs were mainly derived from the high-intensity mariculture and shipping in the bay, as well as industrial and human activities along the surrounding coast. The abundance of MPs in water of the left (western) bay (0.39 items/m3) was considerably higher than that of the right (north-eastern) bay (0.07 items/m3) due to the different levels of population and economic development on the left and right coasts. The ecological risk assessment showed generally low to medium risk from MPs pollution in Yueqing Bay, with higher ecological risk index (H) and potential ecological hazards (RI) of MPs polymers in water samples. These data emphasize the need for timely and effective action to reduce the contribution of intensive human activities to MPs pollution and provide information for further ecotoxicological studies, pollution control, and policy development of MPs.

Peptide-graphene logic sensing system for dual-mode detection of exosomes, molecular information processing and protection.

Peptides with highly sequence-dependent recognition, assembly, and encoding abilities can perform functions similar to DNA or even better, such as biosensing, molecular information processing, coding, or storage. However, the combination of versatile peptides and 2D materials are rarely used for multipurpose integrated applications, including biosensing, information processing and security. Herein, peptide-graphene sensing system was comprehensively used for dual-signal sensing of tumor-derived exosomes (TDEs), logic computing, and information protection. The system used fluorescent-labeled CD63-binding peptide CP05 and graphene oxide (GO) to selectively detect CD63 and TDEs by fluorescence and resonance light scattering. From three levels such as matter, energy, and information analysis, the matter and energy changes in GO-CP05 peptide sensing system were transformed into valuable information, which achieve the dual-mode quantitative detection of TDEs and its marker CD63, and the actual serum analysis. This matter-energy interaction network was also informationized, and utilized for parallel and batch logic computing, two kinds of molecular crypto-steganography (based on peptide sequence and Boolean logic relationships), which facilitates development of intelligent sensing and advanced information technology. This work not only provides a new method for sensitive detection of important disease markers, but also provides ideas for integrating molecular sensing and informatization to open molecular digitization.

Surface restructuring Prussian blue analog-derived bimetallic CoFe phosphides by N-doped graphene quantum dots for electroactive hydrogen evolving catalyst.

As a crucial stage of electrochemical water splitting, hydrogen evolution reaction (HER) favour catalyst to attain rapid kinetics for its broader application, alternating Pt in the acidic environment. Transition metal phosphides (TMPs) are one kind of earth-abundant, nonprecious-based catalyst which has been classified as a viable alternative and active for HER. While the performance remains inferior to Pt which primarily targets durability under high current density, pinpointing the reconfiguration strategy would be critical to their catalytic competency. Herein, we reported engineered N-doped graphene quantum dots (NGQD) on the surface of bimetallic CoFe phosphide (CoFeP) derived from cobalt iron Prussian blue analogue (CoFePBA) as an efficient HER. By introducing the NGQD, the surface architect and electronic state of the transition metal are altered through an adjusted electronic configuration and thus, improving the electrocatalytic activity for HER. The X-ray absorption spectroscopy (XAS) highlighting the role of NGQD, which successfully induced the electron density of Co atoms, further expedites its conductivity and electroactivity. The optimized NGQD/CoFeP substantially surpasses an overpotential of 70 mV (vs. RHE) at the current density of 10 mA cm-2 in 0.5 M H2SO4. Furthermore, the NGQD/CoFeP maintains its exceptional stability under an extremely high current density of 600 mA cm-2 after 12 h of continuous operation. Our findings show that NGQD/CoFeP might demonstrate as a viable alternative to the conventional Pt electrocatalyst in commercial water splitting for hydrogen generation.

Determinants of organophosphate esters exposure in pregnant women from East China.

Organophosphate esters (OPEs) have been broadly used in various industrial and consumer products, resulting in global distribution and human exposure. Gestational exposure to OPEs may adversely affect the health of both pregnant women and their offspring. To better understand OPE exposure in pregnant women, our study determined eight urinary metabolites of major OPEs in pregnant women (n = 733) recruited at 12-16 weeks of gestation from Shanghai, China, and explored the determinants of OPE exposure among various sociodemographic characteristics, lifestyles, and dietary factors. Urinary metabolites of OPEs, including bis (1,3-dichloro-2-propyl) phosphate (BDCPP), bis (2-chloroethyl) phosphate (BCEP), bis (1-chloro-2-propyl) phosphate (BCIPP), dicresyl phosphate (DCP), diphenyl phosphate (DPP), dibutyl phosphate (DBP), bis (2-ethylhexyl) phosphate (BEHP), and bis (2-butoxyethyl) phosphate (BBOEP), exhibited a detection rate ranging from 69.30% to 99.32%. Multivariate linear regression models indicated that pregnant women who were multiparous, had a higher family income per capita, worked in white-collar jobs, and took nutritional supplements such as milk powder and fish oil tended to have higher urinary OPE metabolite concentrations. Besides, independent of sociodemographic characteristics and lifestyle factors, consumption of more aquatic products, soy products, pork, and puffed food, as well as drinking of purified tap water versus tap water, were associated with increased urinary OPEs metabolite concentrations. Our study demonstrated that OPE exposure was ubiquitous in pregnant women from Shanghai, and provided new insights into the potential factors influencing OPE exposure during pregnancy.

Fe/S oxidation-coupled arsenic speciation transformation mediated by AMD enrichment culture under different pH conditions.

Arsenic (As) speciation transformation in acid mine drainage (AMD) is comprehensively affected by biological and abiotic factors, such as microbially mediated Fe/S redox reactions and changes in environmental conditions (pH and oxidation-reduction potential). However, their combined impacts on arsenic speciation transformation remain poorly studied. Therefore, we explored arsenic transformation and immobilization during pyrite dissolution mediated by AMD enrichment culture under different acidic pH conditions. The results for incubation and mineralogical transformation of solid residues show that in the presence of AMD enrichment culture, pH 2.0, 2.5, and 3.0 are more conducive to the formation of jarosites and ferric arsenate, which could immobilize high quantities of dissolved arsenic by adsorption and coprecipitation. The pH conditions significantly affect the initial adsorption of microbial cells to the minerals and the evolution of microbial community structure, further influencing the biodissolution of pyrite and the release and oxidation process of Fe/S. The results of Fe/S/As speciation transformation of the solid residues show that the transformation of Fe, S, and As in solution is mainly regulated by pH and potential values, which imposed significantly different effects on the formation of secondary minerals and thus arsenic oxidation and immobilization. The above results indicated that arsenic transformation is closely related to the Fe/S oxidation associated with pyrite bio-oxidation, and this correlation is critically regulated by the pH conditions of the system.

Soil physical structure drives N-glycan mediated trophic interactions in soil amoebae: Mechanisms and environmental implications.

Soil protozoa are an essential part of the terrestrial ecosystem, playing a vital role in the global element cycling and energy flow. However, one research gap is what are the key factors driving their diversity and environmental fates. In this study, we hypothesized that soil texture could affect soil protozoa's predation and their interactions with environmental pollutants, and we tested it by using a soil amoeba Dictyostelium discoideum as a model system. We found that soil texture affected amoeba's growth and development. In addition, environmental factors cannot explain the variation of amoeba's fitness in different soil textures. Soil sandy particles and water content rather than particle size contribute to amoeba's fitness. Furthermore, different soil textures induced distinct transcriptional responses to amoebae, especially N-glycan-related and multiple signaling pathways and the expression of key genes (e.g., Ras superfamily, cxgE, trap1). The expression of N-glycan-related pathways, which is positively correlated with amoeba predation, was inhibited in sand soil, decreasing amoeba's fitness. Finally, the results showed that soil texture also affects amoeba's interaction with environmental pollutants. In conclusion, this study shows that soil physical structures affect amoeba's interactions with bacteria and environmental pollutants. SYNOPSIS: Soil texture affects soil protozoa's growth and development and their interactions with environmental pollutants.

Soil protists are more resilient to the combined effect of microplastics and heavy metals than bacterial communities.

Heavy metals and micro-/nanoplastic pollution seriously threaten the environment and ecosystems. While many studies investigated their effects on diverse microbes, few studies have focused on soil protists, and it is unclear how soil protists respond to the combined effect of micro-/nanoplastics and heavy metals. This study investigated how soil protistan and bacterial communities respond to single or combined copper and micro-/nanoplastics. The bacterial community exhibited an instantaneous response to single copper pollution, whereas the combined pollution resulted in a hysteresis effect on the protistan community. Single and combined pollution inhibited the predation of protists and changed the construction of ecological networks. Though single and combined pollution did not significantly affect the overall community structure, the exposure experiment indicated that combined pollution harmed soil amoeba's fitness. These findings offer valuable new insights into the toxic effects of single and combined pollution of copper and plastics on soil protistan and bacterial communities. Additionally, this study shows that sequencing-based analyses cannot fully reflect pollutants' adverse effects, and both culture-independent and dependent methods are needed to reveal the impact of pollutants on soil microbes.

Response mechanism of sediment endogenous phosphorus release to functional microorganisms and its cyanobacterial growth and disappearance effects.

Endogenous phosphorus (P) release from lake sediments is an important factor in the eutrophication of overlying waters, as P is the limiting nutrient salt affecting cyanobacterial growth. Microorganisms are also key to the evolution of cyanobacterial growth and disappearance, as they can influence the release of endogenous P. Meanwhile, endogenous phosphorus can also have an impact on microbial structure. However, there is a lack of studies on the response mechanisms between endogenous P release and microorganisms, as well as the exploration of endogenous P release on the whole cyanobacterial growth and disappearance evolution process. In this study, metagenome sequencing was used to characterize the microbial community structure at different times and to explain the P cycle from the perspective of functional genes. The results showed that the number of sediment microorganisms (genes) gradually increased with the P release capacity, and the outbreak with the strongest P release capacity possessed the most abundant microorganisms (genes). Proteobacteria with P solubilizing functions were consistently the most abundant phylum in all four periods and were positively correlated with P release potential assessment factors EPC0, EPC0F, and NAP. Functional genes affect the P cycle by acting primarily on inorganic P solubilization, organic P mineralization, and P transport. These P-functional genes are mainly found in Proteobacteria, Acidobacteria, Chloroflexi, and Actinobacteria microorganisms. In addition, the P form in the sediments was dominated by IP, with the highest concentration (704.86 mg/kg) occurring during the dormant period. Sediments from this period acted as a strong P "sink", creating a precondition for cyanobacterial recovery and outbreaks to provide a source of P. The results of this study can provide a theoretical basis for controlling endogenous P release at the microscopic level of cyanobacterial growth and disappearance.

Dynamic Contrast-Enhanced (DCE) MRI.

The non-invasive dynamic contrast-enhanced MRI (DCE-MRI) method provides valuable insights into tissue perfusion and vascularity. Primarily used in oncology, DCE-MRI is typically utilized to assess morphology and contrast agent (CA) kinetics in the tissue of interest. Interpretation of the temporal signatures of DCE-MRI data includes qualitative, semi-quantitative, and quantitative approaches. Recent advances in MRI technology allow simultaneous high spatial and temporal resolutions in DCE-MRI data acquisition on most vendor platforms, enabling the more desirable approach of quantitative data analysis using pharmacokinetic (PK) modeling. Many technical factors, including signal-to-noise ratio, temporal resolution, quantifications of arterial input function and native tissue T1, and PK model selection, need to be carefully considered when performing quantitative DCE-MRI. Standardization in data acquisition and analysis is especially important in multi-center studies.

Layered double hydroxide loaded pinecone biochar as adsorbent for heavy metals and phosphate ion removal from water.

This study modified pinecone biochar with a novel layered double hydroxide (LDH) to enhance its capacity to adsorb heavy metal and phosphate ions from water. The unmodified pinecone biochar demonstrated moderate adsorption capacities for Cu2+ (25.0 mg/g), Co2+ (24 mg/g), Pb2+ (22.9 mg/g), and phosphate (36.0 mg/g). However, after LDH modification, the LDH-biochar showed significantly improved adsorption capacities for Pb2+ (135.9 mg/g) and phosphate (160.8 mg/g) and a slight increase for Cu2+ (30.6 mg/g) and Co2+ (28.0 mg/g). The LDH layer enhances the adsorption of Pb2+ through ion exchange, phosphate through surface precipitation, and Cu2+ and Co2+ through surface complexation. The integration of LDH creates a multifaceted layer that utilizes various mechanisms to boost the biochar's adsorption capacity.

Reprogramming macrophages via immune cell mobilized hydrogel microspheres for osteoarthritis treatments.

Regulating macrophage activation precisely is crucial in treating chronic inflammation in osteoarthritis (OA). However, the stable pro-inflammatory state and deep distribution of macrophages in vivo pose a great challenge to treatment. In this study, inspired by the innate immune, immune cell mobilized hydrogel microspheres were constructed by microfluidic methods and load chemokines, macrophage antibodies and engineered cell membrane vesicles (sEVs) via covalent and non-covalent junctions. The immune cell mobilized hydrogel microspheres, based on a mixture of streptavidin grafted hyaluronic acid methacrylate (HAMA-SA) and Chondroitin sulfate methacrylate (ChSMA) microspheres (HCM), can recruit, capture and reprogram proinflammatory macrophages in the joint cavity to improve the joint inflammatory microenvironment. In vitro experiments demonstrated that immune cell mobilized hydrogel microspheres had excellent macrophage recruitment, capture, and reprogramming abilities. Pro-inflammatory macrophages can be transformed into anti-inflammatory macrophages with an efficiency of 88.5 %. Animal experiments also revealed significant reduction in synovial inflammation and cartilage matrix degradation of OA. Therefore, the immune cell mobilized hydrogel microspheres may be an effective treatment of OA inflammation for the future.

Visualized test of environmental water pollution and meat freshness: Design of Au NCs-CDs-test paper/PVA film for ratiometric fluorescent sensing of sulfide.

Hydrogen sulfide (H2S) is an environmental pollutant, and also the major released gas during the decay of meat products. To protect the ecological environment and human health, the establishment of a swift, convenient, and accurate detection method for H2S becomes essential. However, existing methods are still suffering from complex synthesis, high toxicity, poor visualization, and high detection limit. Herein, Au NCs-CDs nanocomposite-based test paper and polyvinyl alcohol (PVA) film are combined with a smartphone for sensitive and specific sulfide visualized monitoring. After the addition of sulfide, the fluorescence color changes from orange to green, achieving a quantitative linearity towards sulfide from 5 nM to 30 µM, with a low detection limit of 4.20 nM. The proposed method shows practicability in natural water samples. Furthermore, distinct fluorescence color variation is shown towards H2S originating from spoiled meat, showing the potential application prospect of Au NCs-CDs-PVA film as a meat freshness detector.

Effect of tumor exosome-derived Lnc RNA HOTAIR on the growth and metastasis of gastric cancer

Purpose HOX transcribed antisense RNA (HOTAIR) is a long noncoding RNA (LncRNA) that promotes tumor progression. Exosomes are critically involved in cancer progression. The presence of HOTAIR in the circulating exosomes and the roles of exosomal HOTAIR in gastric cancer (GC) remains unknown. This study aimed to investigate the role of HOTAIR in exosomes in promoting the growth and metastasis of GC. Methods Serum exosomes from GC patients were captured by CD63 immunoliposome magnetic spheres (CD63-IMS), and the biological characteristics of the exosomes were identified. The expression levels of HOTAIR in GC cells, tissues, serum and serum exosomes were detected by fluorescence quantitative PCR (qRT-PCR), and the clinicopathological correlation was statistically analyzed. The growth and metastasis abilities of GC cells with HOTAIR knockdown in vitro were evaluated by cell experiment. The effects of HOTAIR highly-expressed NCI-N87 cell-derived exosomes were used to treat HOTAIR lowly-expressed MKN45 cells on GC growth and metastasis were also evaluated. Results The exosomes isolated by CD63-IMS had a particle size of 89.78 ± 4.8 nm and were oval membranous particles. The expression of HOTAIR in tumor tissues and serum of GC patients was increased (P < 0.05), and the expression of HOTAIR in serum exosomes was significantly increased (P < 0.01). The in NCI-N87 and MKN45 cell experiment demonstrated that HOTAIR knockdown by RNA interference suppressed cell growth and metastasis in NCI-N87 cells. Coculture of exosomes secreted by NCI-N87 cells with MKN45 cells significantly increased the expression of HOTAIR, and enhanced cell growth and metastasis. Conclusion LncRNA HOTAIR can be used as a potential biomarker which provides a new way for the diagnosis and treatment of GC (AU)

Bottom-Up Magnesium Deposition Induced by Paper-Based Triple-Gradient Scaffolds Toward Flexible Magnesium Metal Batteries.

The development of advanced magnesium metal batteries (MMBs) has been hindered by longstanding challenges, such as the inability to induce uniform Mg nucleation and the inefficient utilization of Mg foil. This study introduces a novel solution in the form of a flexible, lightweight, paper-based scaffold that incorporates gradient conductivity, magnesiophilicity, and pore size. This design was achieved through an industrially adaptable papermaking process in which the ratio of carboxylated multi-walled carbon nanotubes to softwood cellulose fibers was meticulously adjusted. The triple-gradient structure of the scaffold enabled the regulation of Mg ion flux, promoting bottom-up Mg deposition. Owing to its high flexibility, low thickness, and reduced density, the scaffold has potential applications in flexible and wearable electronics. Accordingly, the triple-gradient electrodes exhibited stable operation for over 1200 h at 3 mA cm-2 /3 mAh cm-2 in symmetrical cells, markedly outperforming the non-gradient and metallic Mg alternatives. Notably, this study marks the first successful fabrication of a flexible MMB pouch full cell, achieving an impressive volumetric energy density of 244 Wh L-1 . The simplicity and scalability of the triple-gradient design, which uses readily available materials through an industrially compatible papermaking process, open new doors for the production of flexible, high-energy-density metal batteries. This article is protected by copyright. All rights reserved.

Isoproterenol induces MD2 activation by ß-AR-cAMP-PKA-ROS signalling axis in cardiomyocytes and macrophages drives inflammatory heart failure.

Cardiac inflammation contributes to heart failure (HF) induced by isoproterenol (ISO) through activating ß-adrenergic receptors (ß-AR). Recent evidence shows that myeloid differentiation factor 2 (MD2), a key protein in endotoxin-induced inflammation, mediates inflammatory heart diseases. In this study, we investigated the role of MD2 in ISO-ß-AR-induced heart injuries and HF. Mice were infused with ISO (30 mg·kg-1·d-1) via osmotic mini-pumps for 2 weeks. We showed that MD2 in cardiomyocytes and cardiac macrophages was significantly increased and activated in the heart tissues of ISO-challenged mice. Either MD2 knockout or administration of MD2 inhibitor L6H21 (10 mg/kg every 2 days, i.g.) could prevent mouse hearts from ISO-induced inflammation, remodelling and dysfunction. Bone marrow transplantation study revealed that both cardiomyocyte MD2 and bone marrow-derived macrophage MD2 contributed to ISO-induced cardiac inflammation and injuries. In ISO-treated H9c2 cardiomyocyte-like cells, neonatal rat primary cardiomyocytes and primary mouse peritoneal macrophages, MD2 knockout or pre-treatment with L6H21 (10 µM) alleviated ISO-induced inflammatory responses, and the conditioned medium from ISO-challenged macrophages promoted the hypertrophy and fibrosis in cardiomyocytes and fibroblasts. We demonstrated that ISO induced MD2 activation in cardiomyocytes via ß1-AR-cAMP-PKA-ROS signalling axis, and induced inflammatory responses in macrophages via ß2-AR-cAMP-PKA-ROS axis. This study identifies MD2 as a key inflammatory mediator and a promising therapeutic target for ISO-induced heart failure.

Bacterial Flagellum-Drug Nanoconjugates for Carrier-Free Immunochemotherapy.

The combination of immunotherapy and chemotherapy to ablate tumors has attracted substantial attention due to the ability to simultaneously elicit antitumor immune responses and trigger direct tumor cell death. However, conventional combinational strategies mainly focus on the employment of drug carriers to deliver immunomodulators, chemotherapeutics, or their combinations, always suffering from complicated preparation and carrier-relevant side effects. Here, the fabrication of bacterial flagellum-drug nanoconjugates (FDNCs) for carrier-free immunochemotherapy is described. FDNCs are simply prepared by attaching chemotherapeutics to amine residues of flagellin through an acid-sensitive and traceless cis-aconityl linker. By virtue of native nanofibrous structure and immunogenicity, bacterial flagella not only show long-term tumor retention and highly efficient cell internalization, but also provoke robust systemic antitumor immune responses. Meanwhile, conjugated chemotherapeutics exhibit an acid-mediated release profile and durable intratumoral exposure, which can induce potent tumor cell inhibition via direct killing. More importantly, this combination is able to augment immunoactivation effects associated with chemotherapy-enabled immunogenic tumor cell death to further enhance antitumor efficacy. By leveraging the innate response of the immune system to pathogens, the conjugation of therapeutic agents with self-adjuvant bacterial flagella provides an alternative approach to develop carrier-free nanotherapeutics for tumor immunochemotherapy.

Depleting LCAT Aggravates Atherosclerosis in LDLR-deficient Hamster with Reduced LDL-Cholesterol Level.

INTRODUCTION: Lecithin cholesterol acyltransferase (LCAT) plays a crucial role in acyl-esterifying cholesterol in plasma, which is essential for reverse cholesterol transport (RCT). Previous studies indicated that its activity on both α and ß lipoproteins interpret its effects on lipoproteins for many controversial investigations of atherosclerosis. OBJECTIVES: To better understand the relationship between LCAT, diet-induced dyslipidemia and atherosclerosis, we developed a double knockout (LCAT-/-&LDLR-/-, DKO) hamster model to evaluate the specific role of LCAT independent of LDL clearance effects. METHODS: Plasma triglyceride (TG), total cholesterol (TC), high-density lipoprotein-cholesterol (HDL-C), and free cholesterol (FC) levels were measured using biochemical reagent kits. FPLC was performed to analyze the components of lipoproteins. Apolipoprotein content was assessed using western blotting (WB). The hamsters were fed a high cholesterol/high fat diet (HCHFD) to induce atherosclerosis. Oil Red O staining was employed to detect plaque formation. Peritoneal macrophages were studied to investigate the effects of LCAT on cholesterol uptake and efflux. RESULTS: On HCHFD, DKO hamsters exhibited significantly elevated levels of TG and FC, while HDL-C was nearly undetectable without affecting TC levels, as compared to low-density lipoprotein receptor (LDLR)-deficient (LDLR-/-, LKO) hamsters. Lipoprotein profiling revealed a marked increase in plasma chylomicron/very low-density lipoprotein (CM/VLDL) fractions, along with an unexpected reduction in LDL fraction in DKO hamsters. Furthermore, DKO hamsters displayed aggravated atherosclerotic lesions in the aorta, aortic root, and coronary artery relative to LKO hamsters, attributed to a pro-atherogenic lipoprotein profile and impaired cholesterol efflux in macrophages. CONCLUSIONS: Our study demonstrates the beneficial role of LCAT in inhibiting atherosclerotic development and highlights the distinctive lipid metabolism characteristics in hamsters with familial hypercholesterolemia.

Analysis of spike and accessory 3c genes mutations of less virulent and FIP-associated feline coronaviruses in Beijing, China.

Mutations in S and 3c genes of feline coronavirus (FCoV) have been associated with the development of feline infectious peritonitis (FIP). In the present study, FCoV S and 3c genes mutations were analyzed in healthy and FIP cats. M1058L mutation was found in 13.64% (3/22) feces from FIP cats, but not in feces from healthy cats (0/39). The intact 3c gene was found in feces from both healthy cats (19/19) and FIP cats (12/12). All parenteral samples from FIP cats carried one or more of the M1058L mutation, S1060A mutation and mutated 3c gene. FCoV reverse-transcriptase polymerase chain reaction (RT-PCR) of parenteral samples (including ascites, pleural effusions and tissue) is recommended as the gold standard for clinical diagnosis of FIP rather than detection of the M1058L mutation, but when cats have severe gastrointestinal symptoms and lesions, detection of the M1058L mutation in feces may be helpful in diagnosing FIP.