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1.
Epigenomics ; 13(1): 15-30, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33356543

RESUMO

Aim: To develop a trans-omics-based molecular clinicopathological algorithm for predicting pancreatic adenocarcinoma prognosis, we performed a comprehensive analysis of the expression levels of mRNA, DNA methylation and DNA copy number in The Cancer Genome Atlas dataset. Materials & methods: Based on the least absolute shrinkage and selection operator method - COX regression analysis, a trans-omics-based classifier was established to predict overall survival. Nomogram was constructed by combining the classifier band clinical pathological characterization. Results: Based on trans-omics, we developed a 10-gene-based classifier and a molecular-clinicopathologic nomogram for predicting overall survival with satisfactory accuracy. Conclusion: Trans-omics-based classifier and molecule-clinicopathological nomogram based on the classifier can accurately predict the prognosis of pancreatic adenocarcinoma patients.

2.
ACS Appl Mater Interfaces ; 12(47): 53021-53028, 2020 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-33170610

RESUMO

As a well-known electron-withdrawing group, benzo[c][1,2,5]thiadiazole (BT) has been intensively studied and adopted to construct polymer donors with tunable band gaps. However, polymer solar cells (PSCs) with BT-based polymer donors, limited by the weak absorption and inflexible energy level of fullerene derivatives, usually suffer mediocre power conversion efficiencies (PCEs). Here, through subtly tailoring a BT unit with asymmetric fluoro and alkyloxy groups and judiciously pairing a BT-based polymer donor with three narrow band gap non-fullerene acceptors (e.g., IEICO-4F, ITOIC-2F, and IDTCN-O), active layers with complementary absorption spectra, small lowest unoccupied molecular orbital (LUMO) offsets, and preferred morphologies have been achieved. Consequently, PSCs with excellent Jsc values (over 20 mA/cm2) and high PCEs up to 12.33% have been obtained. To the best of our knowledge, the value of 12.33% is among the highest PCEs for BT-based polymers in binary PSCs so far. This work demonstrates that the cooperative effect of energy levels, absorption spectra, and morphologies between the donors and acceptors is crucial for governing the performance of organic photovoltaics.

3.
Bioengineered ; 11(1): 1368-1381, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33200655

RESUMO

Utilizing genomic data to predict cancer prognosis was insufficient. Proteomics can improve our understanding of the etiology and progression of cancer and improve the assessment of cancer prognosis. And the Clinical Proteomic Tumor Analysis Consortium (CPTAC) has generated extensive proteomics data of the vast majority of tumors. Based on CPTAC, we can perform a proteomic pan-carcinoma analysis. We collected the proteomics data and clinical features of cancer patients from CPTAC. Then, we screened 69 differentially expressed proteins (DEPs) with R software in five cancers: hepatocellular carcinoma (HCC), children's brain tumor tissue consortium (CBTTC), clear cell renal cell carcinoma (CCRC), lung adenocarcinoma (LUAD) and uterine corpus endometrial carcinoma (UCEC). GO and KEGG analysis were performed to clarify the function of these proteins. We also identified their interactions. The DEPs-based prognostic model for predicting over survival was identified by least absolute shrinkage and selection operator (LASSO)-Cox regression model in training cohort. Then, we used the time-dependent receiver operating characteristics analysis to evaluate the ability of the prognostic model to predict overall survival and validated it in validation cohort. The results showed that the DEPs-based prognostic model could accurately and effectively predict the survival rate of most cancers.

4.
Clin Chim Acta ; 511: 50-58, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32950519

RESUMO

As more studies have focused on the function of 14-3-3 proteins, their role in tumor progression has gradually improved. In the 14-3-3 protein family, 14-3-3σ is the protein that is most associated with tumor occurrence and development. In some malignancies, 14-3-3σ acts as a tumor suppressor via p53 and tumor suppressor genes. In most tumors, 14-3-3σ overexpression increases resistance to chemotherapy and radiotherapy and mediates the G2-M checkpoint after DNA damage. Although 14-3-3σ overexpression has been closely associated with poorer prognosis in pancreatic, gastric and colorectal cancer, its role in gallbladder and nasopharyngeal cancer remains less clear. As such, the function of 14-3-3σ in specific cancer types needs to be further clarified. It has been hypothesized that a role may be related to its molecular chaperone function combined with various protein ligands. In this review, we examine the role of 14-3-3σ in tumor development and drug resistance. We discuss the potential of targeting 14-3-3σ regulators in cancer therapy and treatment.

5.
J Colloid Interface Sci ; 579: 607-618, 2020 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-32645528

RESUMO

Layered double hydroxides (LDHs) are a kind of classic pseudocapacitive materials with lamellar structure and large specific surface area, which have attracted swinging attention in the electrochemical energy storage area. The CoS2@Ni is synthesized through a hydrothermal process, followed by surface generation of the flower-like nickel-iron layered double hydroxide (NiFe-LDH) nanospheres through a hydrothermal process, which is directly used to design a binder-free electrode with a splendid capacitance capability. The as-synthesized NiFe-LDH@CoS2@Ni electrode presents an outstanding specific capacitance of 11.28 F cm-2 (3880 F g-1) at 2 mA cm-2 (1.17 A g-1) in a three electrodes system. Also, the all-solid-state asymmetric supercapacitor (ASC) is combined utilizing the NiFe-LDH@CoS2@Ni hybrid as the positive electrodes and active carbon covered Ni foam as negative electrodes, respectively. The as-fabricated ASC exhibits a high energy density of 15.84 Wh kg-1 at the power density of 375.16 W kg-1 and can be able to lighten a blue LED indicator for more than 30 min, revealing that the prepared NiFe-LDH@CoS2@Ni owns great potential in the aspect of practical applications. Therefore, the prepared NiFe-LDH@CoS2@Ni with outstanding electrochemical properties could be applied for high-performance supercapacitors.

7.
Aging (Albany NY) ; 12(13): 12896-12920, 2020 06 30.
Artigo em Inglês | MEDLINE | ID: mdl-32611831

RESUMO

BACKGROUND: Emerging evidence suggests that long non-coding RNA (lncRNA) plays a crucial part in the development and progress of hepatocellular carcinoma (HCC). The objective was to develop novel molecular-clinicopathological prediction methods for overall survival (OS) and recurrence of HCC. RESULTS: An 8-lncRNA-based classifier for OS and a 14-lncRNA-based classifier for recurrence were developed by LASSO COX regression analysis, both of which had high accuracy. The tdROC of OS-nomogram and recurrence-nomogram indicates the satisfactory accuracy and predictive power. The classifiers and nomograms for predicting OS and recurrence of HCC were validated in the Test and GEO cohorts. CONCLUSIONS: These two lncRNA-based classifiers could be independent prognostic factors for OS and recurrence. The molecule-clinicopathological nomograms based on the classifiers could increase the prognostic value. METHODS: HCC lncRNA expression profiles from the cancer genome atlas (TCGA) were randomly divided into 1:1 training and test cohorts. Based on least absolute shrinkage and selection operator method (LASSO) COX regression model, lncRNA-based classifiers were established to predict OS and recurrence, respectively. OS-nomogram and recurrence-nomogram were developed by combining lncRNA-based classifiers and clinicopathological characterization to predict OS and recurrence, respectively. The prognostic value was accessed by the time-dependent receiver operating characteristic (tdROC) and the concordance index (C-index).

8.
Cancer Cell Int ; 20: 231, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32536819

RESUMO

Background: Emerging evidence suggests that competing endogenous RNAs plays a crucial role in the development and progress of pancreatic adenocarcinoma (PAAD). The objective was to identify a new lncRNA-miRNA-mRNA network as prognostic markers, and develop and validate a multi-mRNAs-based classifier for predicting overall survival (OS) in PAAD. Methods: Data on pancreatic RNA expression and clinical information of 445 PAAD patients and 328 normal subjects were downloaded from The Cancer Genome Atlas (TCGA), International Cancer Genome Consortium (ICGC) and Genotype-Tissue Expression (GTEx). The weighted correlation network analysis (WGCNA) was used to analyze long non-coding RNA (lncRNA) and mRNA, clustering genes with similar expression patterns. MiRcode was used to predict the sponge microRNAs (miRNAs) corresponding to lncRNAs. The downstream targeted mRNAs of miRNAs were identified by starBase, miRDB, miRTarBase and Targetscan. A multi-mRNAs-based classifier was develop using least absolute shrinkage and selection operator method (LASSO) COX regression model, which was tested in an independent validation cohort. Results: A lncRNA-miRNA-mRNA co-expression network which consisted of 60 lncRNAs, 3 miRNAs and 3 mRNAs associated with the prognosis of patients with PAAD was established. In addition, we constructed a 14-mRNAs-based classifier based on a training cohort composed of 178 PAAD patients, of which the area under receiver operating characteristic (AUC) in predicting 1-year, 3-year, and 5-year OS was 0.719, 0.806 and 0.794, respectively. The classifier also shown good prediction function in independent verification cohorts, with the AUC of 0.604, 0.639 and 0.607, respectively. Conclusions: A novel competitive endogenous RNA (ceRNA) network associated with progression of PAAD could be used as a reference for future molecular biology research.

9.
ACS Appl Mater Interfaces ; 12(27): 30627-30634, 2020 Jul 08.
Artigo em Inglês | MEDLINE | ID: mdl-32538621

RESUMO

Tremendous progress has been achieved on organic transistor-based photodetectors; however, because of the nonpositive correlation relationship between the photo/dark current ratio (P) and the gate voltage, the claimed best P, R (photoresponsivity), and D* (detectivity) can hardly be obtained simultaneously at a given gate voltage, which severely compromises the device performance. Here, a light and voltage dually gated transistor based on an organic semiconducting single crystal of 2,6-dithienylanthracene (DTAnt) is developed. Attributing to its very low on/off ratio in the dark and the remarkable increment of mobilities under illumination, this phototransistor shows good performance with a P of 3.83 × 103, R of 1.32 A W-1, and D* of 1.94 × 1012 Jones achieved simultaneously at Vg = -100 V. Besides, the good reversibility and repeatability of its light-responsive behavior allows for the construction of an artificial photonic neuromorphic device with demonstrated synaptic functions, including excitatory postsynaptic current, short/long-term memory , and pair-pulse facilitation/depression.

10.
Clin Chim Acta ; 508: 240-248, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32417214

RESUMO

BACKGROUND: Nasopharyngeal carcinoma (NPC) is a geographical distributed epithelial tumor of head and neck, which is prevalent in east Africa and Asia, especially southern China. Moreover, NPC has an unfavorable clinical effect and is prone to metastasis at an advanced stage. Although the recovery rate of patients has been improved due to concurrent chemoradiotherapy, poor curative effects and low overall survival remain key issues. The precise mechanisms and pivotal regulators of NPC remain still unclear. To improve the therapeutic efficacy, we focused on related-NPC circular RNAs (circRNAs). CircRNAs are a unique type of endogenous non-coding RNAs (ncRNAs) with a covalent closed-loop structure. Their expression is rich, stable and conservative. Different circRNA have specific tissue and developmental stages and can be detected in body fluids. In addition, circRNAs are involved in multiple pathological processes, especially in cancers. In recent years, using high-throughput indicator technology and bioinformatics technology, a large number of circRNAs have been identified in NPC cells and verified to have biological functions and mechanisms of action. This article aims to provide a retrospective review of the latest research on the proliferation and migration of related-NPC circRNA. Specifically, we focused on the roles and mechanisms of circRNAs in the development and progression of NPC. CONCLUSION: CircRNA can act as an oncogene or tumor suppressor gene and participate in NPC progression (e.g., proliferation, apoptosis, migration, and invasion). In short, circRNAs have potential as biomarkers for the diagnosis, prognosis and treatment of NPC.

11.
Chemistry ; 26(29): 6342-6359, 2020 May 20.
Artigo em Inglês | MEDLINE | ID: mdl-32314829

RESUMO

Novel nanomaterials and advanced nanotechnology continuously push forward the rapid development of sustainable energy conversion and storage equipment. An emerging family of two-dimensional transition-metal carbides, nitrides and carbonitrides, also known as MXenes, have attracted increasing attention and in depth investigation. Benefitting from their unique intrinsic properties, MXenes have attracted significant attention and they have been considered as promising candidate materials for the development of environmentally friendly energy resources. A large number of studies show that MXenes have great potential in energy conversion and storage fields. Despite of their exceptional properties, MXenes also have some inherent characteristics, such as low capacities and unstable retention performances, which severely hinder their prospect applications in energy conversion and storage fields. In this Minireview, the latest progress on MXenes and their hybrid composites with small molecules, polymers, carbon or metal ions, and their applications in energy conversion and storage fields is highlighted, including their use in different types of batteries, supercapacitors, hydrogen/oxygen evolution reactions, electromagnetic interference absorption/shielding and solar steam generation. In addition, the critical challenges and further development prospects of MXene-based materials are also introduced.

12.
Int Immunopharmacol ; 82: 106226, 2020 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-32146317

RESUMO

Osteoarthritis is one of the major causes of disability in elderly adults. Chondrocytes are responsible for the formation and remodeling of articular cartilage in joint tissue. The dysfunction of chondrocytes is a significant factor in the development of osteoarthritis. In the current study, we found that theobromine, a constituent of the cacao plant, possesses a preventive effect against interleukin (IL)-1ß-induced chondrocyte dysfunction. Theobromine ameliorates IL-1ß-induced production of cellular reactive oxygen species (ROS) and inflammatory mediators including cyclooxygenase-2 (COX-2) and prostaglandin E2 (PGE2). The presence of theobromine suppresses IL-1ß-induced inducible nitro oxide synthase (iNOS) expression and cellular nitro oxide (NO) production. Theobromine also suppresses IL-1ß-induced production of the pro-inflammatory cytokines tumor necrosis factor-α (TNF-α) and monocyte chemoattractant protein-1 (MCP-1), as well as matrix metalloproteinases (MMP)-3 and MMP-13. Additionally, theobromine mitigates IL-1ß-induced type II collagen degradation. Mechanistically, we show that theobromine inhibits IL-1ß-induced IκBα activation, nuclear factor-κB (NF-κB) protein p65 accumulation, and transfected NF-κB promoter activity, indicating that theobromine suppresses the NF-κB pathway in chondrocytes. Collectively, our study demonstrates that the natural molecule theobromine has a protective effect to counter cytokine-induced chondrocyte dysfunction, implying its beneficial effect in the prevention of osteoarthritis.

13.
Chemistry ; 26(21): 4790-4797, 2020 Apr 09.
Artigo em Inglês | MEDLINE | ID: mdl-32011778

RESUMO

Supercapacitors have attracted tremendous research interest, since they are expected to achieve battery-level energy density, while having a long calendar life and short charging time. Herein, a novel asymmetric supercapacitor has been successfully assembled from NiCo2 S4 nanosheets and spinous Fe2 O3 nanowire modified hollow melamine foam decorated with polypyrrole as positive and negative electrodes, respectively. Owing to the well-designed nanostructure and suitable matching of electrode materials, the assembled asymmetric supercapacitor (ASC) exhibits an extended operation voltage window of 1.6 V with an energy density of 20.1 Wh kg-1 at a power density of 159.4 kW kg-1 . Moreover, the ASC shows stable cycling stability, with 81.3 % retention after 4000 cycles and a low internal resistance of 1.03 Ω. Additionally, a 2.5 V light-emitting diode indicator can be lit up by three ASCs connected in series; this provides evidence of the practical application potential of the assembled energy-storage system. The excellent electrochemical performances should be credited to the significant enhancement of the specific surface area, charge transport, and mechanical stability resulting from the unique 3D morphology.

14.
Onco Targets Ther ; 13: 593-602, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32021301

RESUMO

Background: Increasing evidence indicates that the dysregulation of miRNAs plays a vital role in tumorigenesis and progression of nasopharyngeal carcinoma (NPC). Thus, it is necessary to further investigate the function and mechanism of miRNAs in NPC. Methods: miR-100 expression was analyzed using publicly available databases and then tested using quantitative RT-PCR in NPC tissues and cell lines. MTT and colony formation assays and xenograft tumor model were used to test the NPC cell growth and proliferation abilities while modulating miR-100 expression. The target of miR-100 was predicted with TargetScan and validated with luciferase reporter assay, quantitative RT-PCR, and Western blot. Results: The expression of miR-100 was significantly reduced in NPC tissues and cell lines. Overexpression of miR-100 obviously suppressed NPC cell growth and proliferation, whereas silencing miR-100 promoted NPC cell growth and proliferation in vitro. HOXA1 (homeobox A1) was validated as a direct target of miR-100, and restoring HOXA1 expression could reverse the inhibitive effect of miR-100 on NPC cell growth and proliferation. The mRNA and protein expression of HOXA1 was increased in NPC cell lines. Furthermore, ectopic expression of miR-100 inhibited xenograft tumor growth in vivo. Conclusion: Taken together, our findings suggest that miR-100 could suppress NPC growth and proliferation through targeting HOXA1, providing a novel target for the miRNA-mediated therapy for patients with NPC in the future.

15.
Clin Chim Acta ; 504: 36-42, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32006544

RESUMO

The occurrence, development, infiltration and metastasis of tumors are very complex processes involving the participation of many factors, some of which play a key role. Annexin A1 (ANXA1) is known as an anti-inflammatory protein. However, it has now been recognized to have a broader role beyond the inflammation, including roles in cell proliferation, differentiation, apoptosis, invasion, angiogenesis and metastasis. This review is intended to outline the research surrounding the pathophysiological effects of ANXA1 in tumors and its potential as a therapeutic and diagnostic agent. These studies comprehensively explore the expression changes of ANXA1 in cancer and further explore its mechanism of action in tumors, which is of great clinical significance for the early diagnosis, treatment and prognostic evaluation of tumors.

16.
Curr Mol Pharmacol ; 13(3): 192-205, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31880267

RESUMO

Nasopharyngeal carcinoma (NPC) is a type of head and neck cancer. As a neoplastic disorder, NPC is a highly malignant squamous cell carcinoma that is derived from the nasopharyngeal epithelium. NPC is radiosensitive; radiotherapy or radiotherapy combining with chemotherapy are the main treatment strategies. However, both modalities are usually accompanied by complications and acquired resistance to radiotherapy is a significant impediment to effective NPC therapy. Therefore, there is an urgent need to discover effective radio-sensitization and radio-resistance biomarkers for NPC. Recent studies have shown that Epstein-Barr virus (EBV)-encoded products, microRNAs (miRNAs), long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs), which share several common signaling pathways, can function in radio-related NPC cells or tissues. Understanding these interconnected regulatory networks will reveal the details of NPC radiation sensitivity and resistance. In this review, we discuss and summarize the specific molecular mechanisms of NPC radio-sensitization and radio-resistance, focusing on EBV-encoded products, miRNAs, lncRNAs and circRNAs. This will provide a foundation for the discovery of more accurate, effective and specific markers related to NPC radiotherapy. EBVencoded products, miRNAs, lncRNAs and circRNAs have emerged as crucial molecules mediating the radio-susceptibility of NPC. This understanding will improve the clinical application of markers and inform the development of novel therapeutics for NPC.

17.
ACS Appl Mater Interfaces ; 12(2): 2591-2600, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31865694

RESUMO

The development for environmentally friendly energy conversion and storage equipment has given rise to tremendous research efforts as a result of the growing requirements for environmental friendly resources and the rapid consumption of traditional fossil fuel. Herein, a novel hierarchical CoO/NiO-Cu@CuO heterostructure is successfully devised and synthesized. Cobalt/nickel ions are used to generate novel CoO/NiO-doped laminated CuO nanospheres through the facile in situ wet oxidation combined with cation exchange and calcination strategies. As a result, the electrochemical supercapacitance of the as-prepared CoO/NiO-Cu@CuO electrode can reach 875 C cm-2 (2035 mF cm-2), which exhibits much better electrochemical performance compared to other precursor electrodes at a same current density of 2 mA cm-2. Moreover, an excellent rate capacity of 1395 mF cm-2 (50 mA cm-2) can be achieved when measured at a relative high current density; 90.3% of the initial supercapacitance remains even after 5000 cycles. Furthermore, the as-prepared hierarchical hybrid of laminated CoO/NiO-CuO nanospheres in situ generated on three-dimensional (3D) porous Cu foam is applied to prepare a solid-state asymmetric supercapacitor equipment unit. The fabricated equipment unit shows an energy density of 69.3 W h kg-1 at a power density of 1080 W kg-1. Additionally, the commercially applied 2.5 V light-emitting-diode indicator with blue light can be energized for 4 min when two as-fabricated supercapacitor devices are in series connection. The unique hierarchical heterostructure of the novel laminated nanospheres combined with the 3D grid structure brings about the outstanding electrochemical capacitor performances. This strategy for the fabrication of hierarchical heterostructure electrodes could have an enormous potential for high-performance electrochemical equipment.

18.
Curr Mol Pharmacol ; 12(4): 324-333, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31677258

RESUMO

AIMS: The aim is to study the role of miR-675-5p coded by long non-coding RNA H19 in the development of Nasopharyngeal Cancer (NPC) and whether miR-675-5p regulates the invasion and metastasis of NPC through targeting SFN (14-3-3σ). The study further validated the relationship between H19, miR-675-5p and SFN in NPC and their relationship with the invasion and metastasis of NPC. METHODS: Western blot was used to detect the expression of 14-3-3σ protein in immortalized normal nasopharyngeal epithelial cells NP69 and different metastatic potential NPC cells, 6-10B and 5-8F. At the same time, to find out the relationship between 14-3-3σ protein and the expression of H19 and miR-675-5p, the expression of H19 and miR-675-5p in normal nasopharynx epithelial cells NP69 and varied nasopharyngeal carcinoma cells 6-10B and 5-8F were quantified by real-time PCR. MiR-675-5p mimic and inhibitor were transfected into NPC 6-10B to over-express and down-express miR-675-5p; miR-675-5p mimic negative control and inhibitor negative control were transfected into NPC 6-10B as control groups. The effect of over-expression and down-expression by miR-675-5p on the expression of 14-3-3σ protein was detected by Western blotting. The 3'-UTR segments of SFN, containing miR-675-5p binding sites were amplified by PCR and the luciferase activity in the transfected cells was assayed to detect whether SFN is the direct target of miR-675-5p. Transwell and scratch assays were used to verify the changes in NPC invasion and metastasis ability of mimics and inhibitors transfected with miR-675-5p. RESULTS: The expression of 14-3-3σ protein in normal nasopharynx epithelial cells NP69 is significantly higher than in varied nasopharyngeal carcinoma cells, 6-10B and 5-8F (P<0.05), and the 14-3-3σ protein levels in low-metastatic nasopharyngeal carcinoma cell 6-10B is higher than in high-metastatic nasopharyngeal carcinoma cell 5-8F. The expression of H19 and miR-675-5p are significantly higher in NPC cells than in NP69 cell (P<0.05). The expression of H19 and miR-675-5p in high-Metastatic nasopharyngeal carcinoma cell 5-8F was higher than in low-Metastatic nasopharyngeal carcinoma cell 6-10B. The expression of 14-3-3σ protein in miR-675-5p mimic cells was significantly lower than in mimic NC (negative control) group and blank control group. However, compared with the blank control group, mimic NC showed no significant difference in 14-3-3σ protein between the two groups. The miR-675-5p inhibitor group was significantly higher than the inhibitor NC group and the blank control group (p<0.05), but there was no significant difference in the expression of 14-3-3σ protein in the inhibitor NC group and the blank control group (p>0.05). Dual-luciferase reporter assay system shows the 3'-UTR segments of SFN containing miR-675-5p binding sites. SFN was the target gene of miR-675-5p. CONCLUSION: 14-3-3σ is downregulated in NPC and is involved in the development of NPC. H19 and miR- 675-5p are upregulated in NPC, which is related to the development of NPC. The over-expression of miR- 675-5p inhibits the expression of 14-3-3σ protein. SFN is the target gene of miR-675-5p. MiR-675-5p targets SFN, downregulates its protein expression and promotes the invasion and metastasis of NPC.


Assuntos
Proteínas 14-3-3/genética , Biomarcadores Tumorais/genética , Exorribonucleases/genética , MicroRNAs/genética , Neoplasias Nasofaríngeas/genética , Invasividade Neoplásica/genética , RNA Longo não Codificante/genética , Linhagem Celular Tumoral , Movimento Celular , Regulação Neoplásica da Expressão Gênica , Humanos , Neoplasias Nasofaríngeas/patologia , Invasividade Neoplásica/patologia
19.
Biosci Rep ; 39(12)2019 12 20.
Artigo em Inglês | MEDLINE | ID: mdl-31729530

RESUMO

The present study was designed to investigate the protective effect of moracin on primary culture of nucleus pulposus cells in intervertebral disc and explore the underlying mechanism. Moracin treatment significantly inhibited the LPS-induced inflammatory cytokine accumulation (IL-1ß, IL-6 and TNF-α) in nucleus pulposus cells. And moracin also dramatically decreased MDA activity, and increased the levels of SOD and CAT induced by LPS challenge. Moreover, the expressions of Nrf-2 and HO-1 were decreased and the protein levels of p-NF-κBp65, p-IκBα, p-smad-3 and TGF-ß were increased by LPS challenge, which were significantly reversed after moracin treatments. Moracin treatments also decreased the levels of matrix degradation enzymes (MMP-3, MMP-13) as indicated by RT-PCR analysis. However, Nrf-2 knockdown abolished these protective effects of moracin. Together, our results demonstrated the ability of moracin to antagonize LPS-mediated inflammation in primary culture of nucleus pulposus in intervertebral disc by partly regulating the Nrf2/HO-1 and NF-κB/TGF-ß pathway in nucleus pulposus cells.


Assuntos
Benzofuranos/farmacologia , Heme Oxigenase (Desciclizante)/genética , Inflamação/tratamento farmacológico , Fator 2 Relacionado a NF-E2/genética , Estilbenos/farmacologia , Fator de Crescimento Transformador beta/genética , Humanos , Inflamação/induzido quimicamente , Inflamação/genética , Inflamação/patologia , Lipopolissacarídeos/toxicidade , Metaloproteinase 13 da Matriz/genética , Metaloproteinase 3 da Matriz/genética , NF-kappa B/genética , Núcleo Pulposo/efeitos dos fármacos , Núcleo Pulposo/metabolismo
20.
Cancer Cell Int ; 19: 107, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31049029

RESUMO

Background: Compelling lines of evidence indicate that DNA methylation of non-coding RNAs (ncRNAs) plays critical roles in various tumour progression. In addition, the differential methylation of ncRNAs can predict prognosis of patients. However, little is known about the clear relationship between DNA methylation profile of ncRNAs and the prognosis of pancreatic adenocarcinoma (PAC) patients. Methods: The data of DNA methylation, RNA-seq, miRNA-seq and clinical features of PAC patients were collected from TCGA database. The DNA methylation profile was obtained using the Infinium HumanMethylation450 BeadChip array. LASSO regression was performed to construct two methylation-based classifiers. The risk score of methylation-based classifiers was calculated for each patient, and the accuracy of the classifiers in predicting overall survival (OS) was examined by ROC curve analysis. In addition, Cox regression models were utilized to assess whether clinical variables and the classifiers were independent prognostic factors for OS. The targets of miRNA and the genes co-expressed with lncRNA were identified with DIANA microT-CDS and the Multi-Experiment Matrix (MEM), respectively. Moreover, DAVID Bioinformatics Resources were applied to analyse the functional enrichment of these targets and co-expressed genes. Results: A total of 4004 CpG sites of miRNA and 11,259 CpG sites of lncRNA were screened. Among these CpG sites, 8 CpG sites of miRNA and 7 CpG sites of lncRNA were found with regression coefficients. By multiplying the sum of methylation degrees of the selected CpGs with these coefficients, two methylation-based classifiers were constructed. The classifiers have shown good performance in predicting the survival rate of PAC patients at varying follow-up times. Interestingly, both of these two classifiers were predominant and independent factors for OS. Furthermore, functional enrichment analysis demonstrated that aberrantly methylated miRNAs and lncRNAs are related to calcium ion transmembrane transport and MAPK, Ras and calcium signalling pathways. Conclusion: In the present study, we identified two methylation-based classifiers of ncRNA associated with OS in PAC patients through a comprehensive analysis of miRNA and lncRNA profiles. We are the first group to demonstrate a relationship between the aberrant DNA methylation of ncRNAs and the prognosis of PAC, and this relationship would contribute to individualized PAC therapy.

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