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1.
Artigo em Inglês | MEDLINE | ID: mdl-34850017

RESUMO

CONTEXT: Data of and studies based on exome sequencing for the genetic evaluation of short stature are limited, and more large-scale studies are warranted. Some factors increase the likelihood of a monogenic cause of short stature, including skeletal dysplasia, severe short stature, and small for gestational age (SGA) without catch-up growth. However, whether these factors can serve as predictors of molecular diagnosis remains unknown. OBJECTIVES: We aimed to explore the diagnostic efficiency of the associated risk factors and their exome sequences for screening. DESIGN, SETTINGS, AND PATIENTS: We defined and applied factors that increased the likelihood of monogenic causes of short stature in diagnostic genetic tests based on next-generation sequencing (NGS) in 814 patients with short stature and at least one other factor. RESULTS: Pathogenic/likely pathogenic (P/LP) variants in genes, copy number variations (CNVs), and chromosomal abnormalities were identified in 361 patients. We found P/LP variants among 111 genes, and RASopathies comprised the most important etiology. Short stature combined with other phenotypes significantly increased the likelihood of monogenic cause, including skeletal dysplasia, facial dysmorphism, and intellectual disability, compared with simple severe short stature (<-3 standard deviation scores). We report novel candidate pathogenic genes, KMT2C for unequivocal growth hormone insensitivity and GATA6 for SGA. CONCLUSIONS: Our study identified the diagnostic characteristics of NGS in short stature with different risk factor. Our study provides novel insights into the current understanding of the etiology of short stature in patients with different phenotypes.

2.
Int J Chron Obstruct Pulmon Dis ; 16: 2945-2951, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34737559

RESUMO

Purpose: Persistent chronic inflammation of chronic obstructive pulmonary disease (COPD) is associated with poor outcomes and frequently results in acute exacerbation. Predicting the number of exacerbations is important. Because interleukin 6 (IL-6) plays an important role in inducing and maintaining chronic inflammation, we sought to observe whether IL-6 measurement can predict the frequency of acute exacerbation of COPD. Methods: We reviewed serum IL-6 concentrations of stable COPD patients from January 2016 to December 2017 and statistically analyzed them to determine the optimal threshold value to predict the frequency of COPD acute exacerbations. Outpatients with stable COPD were then recruited between January 2018 and December 2019 and grouped into a low IL-6 group and a high IL-6 group according to this threshold value. We then compared the number of exacerbations of COPD in 1 year between the two groups. Results: We reviewed data from 95 COPD patients, who had a median of 1.00 exacerbations in preceding year; 35 of these patients had no fewer than two. The median IL-6 concentration was 8.80 pg/mL. IL-6 and hs-CRP were positively correlated with frequency of acute exacerbation in the preceding year, COPD assessment test (CAT) score and British medical research council (mMRC) score, and negatively correlated with forced expiratory volume in one second as percentage of predicted value (FEV1%pred) and FEV1/FVC% (forced vital capacity). IL-6 was the risk factor of COPD patients with two or more exacerbations in 1 year. Finally, we enrolled 65 COPD patients and divided into low IL-6 group and high IL-6 group; the high IL-6 group experienced more frequent exacerbations than did the low IL-6 group. Conclusion: An IL-6 measurement of 14.030 pg/mL or more is a risk factor for ≥2 acute exacerbations of COPD in the following year.


Assuntos
Interleucina-6 , Doença Pulmonar Obstrutiva Crônica , Progressão da Doença , Volume Expiratório Forçado , Humanos , Doença Pulmonar Obstrutiva Crônica/diagnóstico , Doença Pulmonar Obstrutiva Crônica/epidemiologia , Testes de Função Respiratória , Capacidade Vital
3.
PLoS Negl Trop Dis ; 15(11): e0009963, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34784371

RESUMO

BACKGROUND: Australia is theoretically at risk of epidemic chikungunya virus (CHIKV) activity as the principal vectors are present on the mainland Aedes aegypti) and some islands of the Torres Strait (Ae. aegypti and Ae. albopictus). Both vectors are highly invasive and adapted to urban environments with a capacity to expand their distributions into south-east Queensland and other states in Australia. We sought to estimate the epidemic potential of CHIKV, which is not currently endemic in Australia, by considering exclusively transmission by the established vector in Australia, Ae. aegypti, due to the historical relevance and anthropophilic nature of the vector. METHODOLOGY/PRINCIPAL FINDINGS: We estimated the historical (1995-2019) epidemic potential of CHIKV in eleven Australian locations, including the Torres Strait, using a basic reproduction number equation. We found that the main urban centres of Northern Australia could sustain an epidemic of CHIKV. We then estimated future trends in epidemic potential for the main centres for the years 2020 to 2029. We also conducted uncertainty and sensitivity analyses on the variables comprising the basic reproduction number and found high sensitivity to mosquito population size, human population size, impact of vector control and human infectious period. CONCLUSIONS/SIGNIFICANCE: By estimating the epidemic potential for CHIKV transmission on mainland Australia and the Torres Strait, we identified key areas of focus for controlling vector populations and reducing human exposure. As the epidemic potential of the virus is estimated to rise towards 2029, a greater focus on control and prevention measures should be implemented in at-risk locations.

4.
Analyst ; 2021 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-34816841

RESUMO

G-quadruplex-based complexes have been widely used in various analytical methods due to their outstanding capabilities of generating colorimetric, fluorescent or electrochemical signals. However, since loop sequences in traditional G-quadruplex structures are quite short, it is difficult to establish biosensors solely using G-quadruplex-based complexes. Herein, we attempted to lengthen the loop sequences of G-quadruplex structures and found that G-quadruplex-hemin DNAzymes (G-DNAzymes) with long loops (even 30 nucleotides) maintain high peroxidase activity. In addition, the peroxidase activity is not affected by the hybridization of the long loop with its complementary counterpart. Consequently, G-DNAzyme can be endowed with an additional function by taking the long loop as a recognition element, which may facilitate the construction of diverse colorimetric biosensors. Furthermore, by designing an apurinic/apyrimidinic site or a complementary sequence of microRNA-21 (miRNA-21) in long loops, bifunctional G-DNAzymes can be split in the presence of apurinic/apyrimidinic endonuclease 1 (APE1) or miRNA-21, decreasing their peroxidase activities. Accordingly, APE1 and miRNA-21 are quantified using 3,3',5,5'-tetramethylbenzidine as a chromophore. Using the G-DNAzyme, APE1 can be detected in a linear range from 2.5 to 22.5 U mL-1 with a LOD of 1.8 U mL-1. It is to be noted that benefitting from duplex-specific nuclease-induced signal amplification, the linear range of the miRNA-21 biosensor is broadened to 5 orders of magnitude, while the limit of detection is as low as 73 fM. This work demonstrates that G-DNAzymes with long loops can both generate signals and recognize targets, providing an alternative strategy to design G-quadruplex-based analytical methods.

5.
Int J Biol Macromol ; 2021 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-34785199

RESUMO

The present study investigated the structural characteristics and its protective effect against H2O2-induced injury fibroblast cells of Bletilla striata tuber polysaccharide. The polysaccharides were gently extracted by water and recovered using the method of alcohol precipitation, and after further purification by DEAE-Sepharose Fast Flow gel column, a pure polysaccharide (pBSP) was finally obtained. The structural characterization of pBSP were investigated by using periodate oxidation studies, Smith-degradation, FT-IR spectroscopy, 1D and 2D NMR spectroscopy. The antioxidant effect of pBSP was evaluated by inhibiting the production of reactive oxygen species (ROS) in human fibroblast model cells induced by H2O2. It was firstly reported that pBSP was composed of d-glucose and D-mannose in a molar ratio of 1.00:1.34 with a molecular weight of 327.6 kDa. The repeating units of pBSP contained (1 â†’ 4)-linked-ß-D-Manp, (1 â†’ 4)-linked-α-D-Glcp and (1 â†’ 3)-linked-ß-D-Manp, and there was no branched chain. pBSP exhibited no toxic effect on fibroblasts cells and could protect them against H2O2-induced injuries. After pretreatment with pBSP for 24 h, the content of ROS in fibroblasts decreased significantly. These results not only confirm the availability B. striata, but also indicate that pBSP have potential antioxidant capacity. Our observations can provide foundation for further development of pBSP-based cosmetics.

6.
Front Oncol ; 11: 732883, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34722275

RESUMO

Purpose: The high intracranial efficacy of targeted therapeutic agents poses a challenge in determining the optimal sequence of local radiation therapy (RT) and systemic treatment with tyrosine kinase inhibitors (TKIs) in non-small cell lung cancer (NSCLC) patients with brain metastasis (BM). Therefore, we conducted a cohort study to elucidate the appropriate treatment strategy, either upfront RT or deferred RT including a toxicity assessment, in these patients. Patients and Methods: We retrospectively evaluated patients with gene-driven BMs from a single institution and divided them into deferred and upfront RT groups. Survival was estimated using a log-rank test. Intracranial progression was estimated using Fine-Gray competing risks model. Cox proportional hazards regression was performed for multivariable analysis in the entire group and subgroups. Results: Among the 198 eligible patients, 94 and 104 patients received deferred and upfront RT, respectively. The upfront RT group showed a lower intracranial progression risk with an adjusted sub-distribution hazard ratios of 0.41 (95% CI, 0.30-0.57) than did the deferred RT group (median intracranial progression-free survival [iPFS], 19.9 months vs. 11.1 months; p < 0.001). The median overall survival (OS; 43.2 months vs. 49.1 months, p = 0.377) and BM-specific survival (92.1 months vs. 82.9 months, p = 0.810) after salvage therapy were not significantly different between the upfront and deferred groups. Among patients with progressed extracranial disease, the deferred RT group showed significantly better OS than did the upfront RT group (44.0 vs. 28.1 months, p = 0.022). Grade 3-4 treatment-related adverse events were rare, and similar toxicities were observed between the two groups. Conclusion: Compared to the deferred RT group, the upfront RT group achieved longer iPFS and similar survival outcomes in most patients with gene-driven NSCLC BM, although patients with progression of extracranial disease might benefit from deferred RT. Both groups showed well-tolerated toxicities. Trial registration ID: NCT04832672.

7.
Ann Palliat Med ; 10(9): 9435-9442, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34628869

RESUMO

BACKGROUND: The increasingly younger age of stroke onset has posed a serious threat to the physical and mental health of young and middle-aged adults. Young and middle-aged stroke patients often suffer from varying degrees of motor, cognitive, and language disabilities, which can trigger considerable psychological trauma. However, these patients may have certain posttraumatic growth (PTG). As a positive psychological experience, PTG provides a new entry point for clinical psychological interventions. Here, we explored the trajectories of PTG in young and middle-aged stroke patients and their predictive effects on mental health. METHODS: A total of in 165 patients who were treated at our center from January to December 2019 were enrolled with randomized sampling. The survey was conducted at 5 times follow up through use of the Posttraumatic Growth Inventory (PTGI), Social Support Rating Scale (SSRS), and Symptom Checklist-90 (SCL-90). Data were analyzed by using the latent growth mixture modeling (LGMM). RESULTS: The PTG levels in young and middle-aged stroke patients showed a gradual increase (F=9.927; P<0.01). According to the relevant parameters of the trajectories, the subjects were divided into the high PTG group (n=43, 26.06%), low PTG group (n=25, 15.15%), rapidly increasing PTG group (n=47, 28.48%), and constantly decreasing PTG group (n=50 cases, 30.30%). Both the initial level and developmental rate of PTG had significantly negative predictive effects on SCL-90 score (B=-0.46, P<0.01; B=-0.31, P<0.01); in other words, they had positive predictive effects on mental health. CONCLUSIONS: There are different trajectories of PTG in young and middle-aged stroke patients, and the initial level and developmental speed of PTG have positive predictive effects on mental health in these patients.


Assuntos
Crescimento Psicológico Pós-Traumático , Acidente Vascular Cerebral , Adaptação Psicológica , Adulto , Humanos , Saúde Mental , Pessoa de Meia-Idade , Apoio Social
8.
Nanotechnology ; 33(7)2021 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-34706353

RESUMO

Wearable sensors are believed to be the most important part of the Internet of Things. In order to meet the application requirements, low-dimensional materials such as graphene and carbon nanotubes have been attempted to constitute wearable sensors with high performance. Our discussions in this review include the different low-dimensional material based sensors which are employed in wearable applications. Low-dimensional materials based wearable sensors for detecting various physical quantities in surroundings, including temperature sensor, pressure or strain sensor and humidity sensor, is introduced. The primary objective of this paper is to provide a comprehensive review of research status and future development direction of low-dimensional materials based wearable sensors. Challenges for developing commercially low-dimensional namomaterials based wearable sensors are highlighted as well.

9.
Artigo em Inglês | MEDLINE | ID: mdl-34552653

RESUMO

Background: Chaiqin Qingning Capsule (CQ-C) is a traditional Chinese medicine (TCM) formula commonly used to treat respiratory infectious diseases in China. The aim of this study was to detect the effect and mechanism of CQ-C treated with influenza virus in vitro and vivo. Methods: The cytotoxicity and antiviral activity of CQ-C in vitro was determined by methyl thiazolyl tetrazolium (MTT) assay. The regulation of CQ-C on cytokine/chemokine expression was evaluated using RT-qPCR. In addition, the effect of CQ-C on the pathway protein, NF-κB, and its phosphorylation level was verified by western blotting. After virus inoculation, BALB/c mice were administered with CQ-C of different concentrations for 7 days. Body weight, viral titer, lung pathology, and mortality of the mice were measured, and the level of inflammatory cytokines was also examined using real-time RT-qPCR. Results: CQ-C inhibited the proliferation of influenza virus of various strains in vitro, with the 50% inhibitory concentration (IC50) ranging from 49 to 59 µg/mL. CQ-C downregulated virus-induced gene expression of IL-6, TNF-α, CXCL8, CXCL10, CCL5, and COX-2 in a dose-dependent manner in A549 cells. Also, CQ-C inhibited the expression of NF-κB protein of the signaling pathway. Moreover, a decrease of the lung index and mortality of mice was observed in the CQ-C (1 g/kg/d) group. The related cytokine/chemokine expression was also decreased in the early stages of infection in the mRNA level. Conclusion: As a clinically applied Chinese prescription, our study shows that CQ-C has a wide range of effects on several influenza viruses. Moreover, CQ-C could play an important role in anti-influenza activity and anti-inflammation in vitro and in vivo. Thus, CQ-C may be a promising treatment option for influenza.

10.
Nanoscale ; 13(36): 15380-15393, 2021 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-34499073

RESUMO

Wearable and highly sensitive pressure sensors are of great importance for robotics, health monitoring and biomedical applications. For simultaneously achieving high sensitivity within a broad working range, fast response time (within a few milliseconds), minimal hysteresis and excellent cycling stability are critical for high performance pressure sensors. However, it remains a major challenge. Herein, we report a conceptual micro-cliff design of a graphene sensor with a record high sensitivity of up to 72 568 kPa-1 in a broad working range of 0-255 kPa, which is one order of magnitude higher than the state-of-the-art reported sensitivity. In addition, the detection limit can be as low as 0.35 Pa and the fast response time is less than 5 ms. The sensor also has a minimal hysteresis and an outstanding cycling stability of 5000 cycles, all of which meet the requirements of an ideal pressure sensor. More interestingly, the micro-cliff graphene sensor is made by the fast and scalable flash reduction of graphene oxide using a single flashlight pulse within 150 ms and has been integrated into a wearable smart insole and an E-glove prototype for demonstration of health monitoring applications. This micro-cliff graphene pressure sensor achieves record-high sensitivity, which brings new possibilities in sensor research and promises broad applications.

11.
Nat Commun ; 12(1): 5673, 2021 09 28.
Artigo em Inglês | MEDLINE | ID: mdl-34584089

RESUMO

Cloning quantitative trait locus (QTL) is time consuming and laborious, which hinders the understanding of natural variation and genetic diversity. Here, we introduce RapMap, a method for rapid multi-QTL mapping by employing F2 gradient populations (F2GPs) constructed by minor-phenotypic-difference accessions. The co-segregation standard of the single-locus genetic models ensures simultaneous integration of a three-in-one framework in RapMap i.e. detecting a real QTL, confirming its effect, and obtaining its near-isogenic line-like line (NIL-LL). We demonstrate the feasibility of RapMap by cloning eight rice grain-size genes using 15 F2GPs in three years. These genes explain a total of 75% of grain shape variation. Allele frequency analysis of these genes using a large germplasm collection reveals directional selection of the slender and long grains in indica rice domestication. In addition, major grain-size genes have been strongly selected during rice domestication. We think application of RapMap in crops will accelerate gene discovery and genomic breeding.


Assuntos
Biologia Computacional/métodos , Grão Comestível/genética , Oryza/genética , Locos de Características Quantitativas/genética , Seleção Genética , Mapeamento Cromossômico/métodos , Cromossomos de Plantas/genética , Produtos Agrícolas/genética , Domesticação , Estudo de Associação Genômica Ampla/métodos , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Oryza/classificação , Fenótipo , Filogenia , Melhoramento Vegetal/métodos , Sementes/genética , Especificidade da Espécie
13.
ACS Appl Mater Interfaces ; 13(34): 40837-40846, 2021 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-34382765

RESUMO

Ga2O3-based solar-blind photodetectors have been extensively investigated for a wide range of applications. However, to date, a lot of research has focused on optimizing the epitaxial technique or constructing a heterojunction, and studies concerning surface passivation, a key technique in electronic and optoelectronic devices, are severely lacking. Here, we report an ultrasensitive metal-semiconductor-metal photodetector employing a ß-Ga2O3 homojunction structure realized by low-energy surface fluorine plasma treatment, in which an ultrathin fluorine-doped layer served for surface passivation. Without inserting/capping a foreign layer, this strategy utilized fluorine dopants to both passivate local oxygen vacancies and suppress surface chemisorption. The dual effects have opposite impacts on device current magnitude (by suppressing metal/semiconductor junction leakage and inhibiting surface-chemisorption-induced carrier consumption) but dominate in dark and under illumination, respectively. By means of such unique mechanisms, the simultaneous improvement on dark and photo current characteristics was achieved, leading to the sensitivity enhanced by nearly 1 order of magnitude. Accordingly, the 15 min treated sample exhibited striking competitiveness in terms of comprehensive properties, including a dark current as low as 6 pA, a responsivity of 18.43 A/W, an external quantum efficiency approaching 1 × 104%, a specific detectivity of 2.48 × 1014 Jones, and a solar-blind/UV rejection ratio close to 1 × 105. Furthermore, the response speed was effectively accelerated because of the reduction on metal/semiconductor interface trap states. Our findings provide a facile, economical, and contamination-free surface passivation technique, which unlocks the potential for comprehensively improving the performance of ß-Ga2O3 solar-blind metal-semiconductor-metal photodetectors.

14.
BMC Med Genomics ; 14(1): 207, 2021 08 21.
Artigo em Inglês | MEDLINE | ID: mdl-34419044

RESUMO

BACKGROUND: Pathogenic variants in POC1A led to SOFT syndrome and variant POC1A-related (vPOC1A) syndrome. SOFT syndrome is a rare primordial dwarfism condition characterized by short stature, onychodysplasia, facial dysmorphism and hypotrichosis.The main clinical differences between SOFT and vPOC1A syndrome include dyslipidemia with insulin resistance and acanthosis nigricans. To our knowledge, this is the first report of a SOFT syndrome patient diagnosed with a homozygous splicing variant, which could help to extend our understanding of the genotypic and phenotypic information of the disease. CASE PRESENTATION: We reported a seven-year-old boy with SOFT syndrome. The patient presented symmetrical short stature and facial features, including prominent forehead, inverted triangular face, epicanthal fold, small teeth and enlarged ears. Laboratory tests displayed mild insulin resistance. Whole-exome sequencing (WES) led to the identification of a homozygous splicing variant (c.981+1G>A) in POC1A gene of the patient, which was inherited from his heterozygous parents confirmed by Sanger sequencing. Further transcriptional experiments of the splicing variant revealed aberrant percentage of exon 9 skipping transcripts. CONCLUSIONS: This is the firstly reported case of a SOFT syndrome patient with a novel homozygous splicing variant and detailed delineation of the aberrant transcript in proband and carrier of the variant in Chinese. Our study enriched mutational spectrum of POC1A which could help in further genetic diagnosis and counselling of SOFT syndrome patients.

15.
Methods Mol Biol ; 2365: 175-184, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34432244

RESUMO

A new drug discovery strategy by inducing the degradation of oncoproteins through ubiquitin-proteasome system (UPS) has gained a lot of traction in the last decade (Verma et al. Mol Cell 77(3):446-460, 2020; Huang, Dixit. Cell Res 26:484, 2016). Multiple degrader platforms, such as IMiDs (Kronke et al. Science 343:301-305, 2014; Lu et al. Science 343:305-309; 2014), PROTAC (proteolysis targeting chimera) (Winter et al. Science 348:1376-1381, 2015), and molecular glues (Tan et al. Nature 446:640-645, 2007), have been approved or currently being developed in clinical trials. Compared to conventional drug inhibitors, degraders have a lot of advantages, such as catalytic mechanisms of action (MOA), no requirement of high-affinity ligands with targets, and potentially more sustained efficacy (Verma et al. Mol Cell 77(3):446-460, 2020; Huang, Dixit. Cell Res 26:484, 2016; Bondeson et al. Nat Chem Biol 11:611-617). Here, we describe protocols that measure intrinsic protein ubiquitination, degrader-induced target protein degradation, and cancer cell proliferation evaluation, as these protocols can help evaluate the potential of a drug target using a degrader platform.

16.
Nano Lett ; 21(20): 8917-8923, 2021 Oct 27.
Artigo em Inglês | MEDLINE | ID: mdl-34459611

RESUMO

Plasmonic metasurfaces supporting collective lattice resonances have attracted increasing interest due to their exciting properties of strong spatial coherence and enhanced light-matter interaction. Although the focusing of light by high-numerical-aperture (NA) objectives provides an essential way to boost the field intensities, it remains challenging to excite high-quality resonances by using high-NA objectives due to strong angular dispersion. Here, we address this challenge by employing the physics of bound states in the continuum (BICs). We design a novel anisotropic plasmonic metasurface combining a two-dimensional lattice of high-aspect-ratio pillars with a one-dimensional plasmonic grating, fabricated by a two-photon polymerization technique and gold sputtering. We demonstrate experimentally multiple resonances with absorption amplitudes exceeding 80% at mid-IR using an NA = 0.4 reflective objective. This is enabled by the weak angular dispersion of quasi-BIC resonances in such hybrid plasmonic metasurfaces. Our results suggest novel strategies for designing photonic devices that manipulate focused light with a strong field concentration.

17.
Materials (Basel) ; 14(12)2021 Jun 17.
Artigo em Inglês | MEDLINE | ID: mdl-34204347

RESUMO

Excessive bleeding induces a high risk of death and is a leading cause of deaths that result from traffic accidents and military conflict. In this paper, we developed a novel porous chitosan-CaCO3 (CS-CaCO3) composite material and investigated its hemostatic properties and wound healing performance. The CS-CaCO3 composites material was prepared via a wet-granulation method. Granulation increases the infiltrating ability of the CS-CaCO3 composites material. The improved water absorption ability was enhanced to 460% for the CS-CaCO3 composites material compared to the CaCO3 or chitosan with only one single component. The coagulation studies in vivo illustrated that the blood clotting time was greatly reduced from 31 s for CaCO3 to 16 s for the CS-CaCO3 composite material. According to the results of the wound healing experiments in rats, it was found that the CS-CaCO3 composite material can promote wound healing. The CS-CaCO3 composite material could accelerate wound healing to a rate of 9 days, compared with 12 days for the CaCO3. The hemostatic activity, biocompatibility, and low cost of CS-CaCO3 composite material make it a potential agent for effective hemostatic and wound healing materials.

18.
J Hazard Mater ; 420: 126609, 2021 10 15.
Artigo em Inglês | MEDLINE | ID: mdl-34329113

RESUMO

To effectively address the serious human health challenges and ecological damage caused by organic dyes in wastewater, we developed a novel bionic adsorbent (LDH@PDA@MPNs) for the selective adsorption and removal of malachite green (MG) and crystalline violet (CV). The adsorbent was prepared using a facile two-step method based on mussel-inspired chemistry and metal complexation. The physicochemical structure, surface morphology, and composition of the LDH@PDA@MPNs were characterized by scanning electron microscopy, Fourier-transform infrared spectrometry, X-ray photoelectron spectroscopy, and thermogravimetric analysis. Adsorption of MG and CV with the LDH@PDA@MPNs was evaluated. Under optimal conditions, the maximum adsorption of MG and CV by the adsorbent was 89.608 and 40.481 mg/g, respectively. The adsorption kinetics showed that the experimental data were in good agreement with the pseudo-second-order kinetic model, and the equilibrium adsorption isotherm data fitted well with the Freundlich model. The thermodynamic results indicated that the adsorption of the dyes on LDH@PDA@MPNs was a spontaneous endothermic process. Importantly, the bionic adsorbent not only shows high removal efficiency by easy regeneration with low-cost reagents but also exhibits high selectivity for dyes in both single and binary systems. Therefore, LDH@PDA@MPNs have the potential to adsorb and remove dyes from complex wastewater solutions.


Assuntos
Corantes , Poluentes Químicos da Água , Adsorção , Biônica , Humanos , Concentração de Íons de Hidrogênio , Hidróxidos , Indóis , Polímeros , Polifenóis , Poluentes Químicos da Água/análise
19.
Neuroscience ; 469: 103-109, 2021 08 10.
Artigo em Inglês | MEDLINE | ID: mdl-34171408

RESUMO

The present study aimed to investigate the association between the serum SIRT1 protein and the severity of spinal cord injury (SCI) as well as the neurological recovery in mice. In this study, the wild-type (WT), Mx1-Cre+ SIRT1loxP/loxP (Mx1), and LCK-Cre+SIRT1loxP/loxP (LCK) mice were subjected to sham surgery, mild, moderate, or severe SCI, respectively. The serum was collected at intervals of 12 h, 1 day (d), 3 d, 5 d, 7 d, 10 d, 14 d, and 21 d after the injury. The locomotor function of all the animals was assessed using the Basso mouse scale (BMS) and the serum SIRT1 proteins were analyzed using enzyme-linked immunosorbent assay (ELISA). The results demonstrated that about 7-10 d after SCI, the levels of SIRT1 protein in the serum correlated significantly with the severity of the injury and at 28 d post-injury, there was a distant neurological recovery (BMS score). The serum SIRT1 concentration in both the Mx1 and LCK mice in the sham group was significantly reduced compared to that in the WT mice, and there was a delayed increase in the serum SIRT1 levels after injury. These findings indicate that the SIRT1 concentrations in the serum of the SCI mice closely correlated with the acute severity and neurological outcome.


Assuntos
Sirtuína 1 , Traumatismos da Medula Espinal , Animais , Camundongos , Recuperação de Função Fisiológica , Medula Espinal
20.
Food Chem ; 362: 130118, 2021 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-34082296

RESUMO

Organophosphate pesticides (OPs) are often used as insecticides and acaricides in agriculture, thus improving yields. OP residues may pose a serious threat, duetoinhibitionof the enzymeacetylcholinesterase(AChE). Therefore, a competitive bio-barcode immunoassay was designed for simultaneous quantification of organophosphate pesticide residues using AuNP signal amplification technology and Au@Pt catalysis. The AuNP probes were labelled with antibodies and corresponding bio-barcodes (ssDNAs), MNP probes coated with ovalbumin pesticide haptens and Au@Pt probes functionalized with the complementary ssDNAs were then prepared. Subsequently, pesticides competed with MNP probes to bind the AuNP probes. The recoveries of the developed assay were ranged from 71.26 to 117.47% with RSDs from 2.52 to 14.52%. The LODs were 9.88, 3.91, and 1.47 ng·kg-1, for parathion, triazophos, and chlorpyrifos, respectively. The assay was closely correlated with the data obtained from LC-MS/MS. Therefore, the developed method has the potential to be used as an alternative approach for detection of multiple pesticides.


Assuntos
Contaminação de Alimentos/análise , Imunoensaio/métodos , Nanopartículas Metálicas/química , Resíduos de Praguicidas/análise , Catálise , Clorpirifos/análise , Cromatografia Líquida , Análise de Alimentos/métodos , Ouro/química , Imunoensaio/instrumentação , Limite de Detecção , Compostos Organofosforados/análise , Organotiofosfatos/análise , Oxazinas/química , Paration/análise , Platina/química , Espectrometria de Massas em Tandem , Triazóis/análise
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