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1.
Eur J Pharmacol ; 874: 172990, 2020 May 05.
Artigo em Inglês | MEDLINE | ID: mdl-32057718

RESUMO

Ovarian carcinoma (OC) begins in the ovaries and remains a highly lethal malignancy. Despite great efforts have been made to fight against OC, there still remain limited therapeutic options owing to chemotherapy drug resistance and serious side effects. Osthole is a derivative of coumarin and extracted from Cnidium monnieri (L.) Cusson, which has been drawn more attention due to its high biological activity in various disease. However, the underlying mechanism of osthole in OC is still unclear. In this study, we aim to evaluate the mechanism of osthole against OC cells. Methodologically, Cell Counting Kit-8 (CCK-8) and LIVE/DEAD™ Cell Imaging experiments were employed to assess cell viability. 2',7'-Dichlorofluorescin diacetate (DCFH-DA) staining, flow cytometry, Hoechst staining, JC-1 staining assay and western blotting were performed to study apoptosis. Transmission electron microscopy, western blotting and monodansyl cadaverine (MDC) staining assay were used to study autophagy. Western blotting and microscopy image were employed to determine pyroptosis. Our results demonstrated that osthole could significantly suppress OC cells growth in a dose-dependent manner. We further proved that osthole could inhibit OC cells growth by mitochondria-mediated apoptosis. Meanwhile, we also discovered that osthole could trigger cell autophagy and lead to cell death. Furthermore, our study revealed that osthole could lead to pyroptosis through inducing the cleavage of gasdermin E (c-GSDME) level. Taken together, Osthole could significantly suppress the growth of OC cells and induce OC cells death via apoptosis, pyroptosis and autophagy, which is a promising new drug for the treatment of OC.

2.
Bioorg Med Chem ; 28(6): 115352, 2020 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-32044229

RESUMO

Magnolol, a major bioactive component found in Magnolia officinalis with anti-inflammation and anti-oxidation activities as well as minimized cytotoxic effects. Although magnolol has a wide range of clinical applications, the anti-tumor activity of magnolol is not efficient. Herein, we reported the synthesis and anti-cancer activities of three novel magnolol analogues CT2-1, CT2-2, CT2-3, among which CT2-3 revealed more efficient anti-non-small cell lung cancer (NSCLC) activity than magnolol. Our data showed that CT2-3 could significantly inhibit the proliferation of human NSCLC cells in a dose-dependent manner. In addition, we revealed CT2-3 could induce cell cycle arrest through down-regulating mRNA expression of CDK4, CDK6 and cyclin D1. Moreover, we verified that CT2-3 could cause ROS generation, leading to apoptosis of human NSCLC cells. Further more, we also provided strong evidences that CT2-3 down-regulates the expression of c-Myc and topoisomerases, and contributes to the apoptosis of human NSCLC cells. Taken together, the current study is the first to report a promising new chemotherapeutic drug candidate CT2-3 that can efficiently eliminate human NSCLC cells through triggering cell cycle arrest as well as ROS-mediated and c-Myc/topoisomerases-mediated apoptosis.

3.
BMJ Open ; 10(1): e032840, 2020 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-31911517

RESUMO

OBJECTIVE: The metabolic syndrome is a major risk factor for cardiovascular disease. Little information exists on the prevalence of the metabolic syndrome at high-altitude areas in China. We aimed to estimate the prevalence of metabolic syndrome and its individual components at high altitude. METHODS: A cross-sectional survey of 5053 adults living in Derong from elevation of 2060 to 3820 m was carried out in 2013. Metabolic syndrome was defined according to the Chinese Diabetes Society criteria. RESULTS: The overall prevalence of metabolic syndrome was 3.6% (5.9% in men and 1.8% in women) in Derong, China. Obesity and hypertension were more prevalent among adults than dyslipidaemia and hyperglycaemia at high altitude. The prevalence of metabolic syndrome was higher in township than countryside residents (6.6%, 11.9% in men and 1.5% in women vs 3.0%, 4.6% in men and 1.8% in women). Men with age 30-59 years old had a much higher prevalence of metabolic syndrome than women. Men, township, middle and old age residents had a higher risk of metabolic syndrome. The risk of obesity and dyslipidaemia decreased and the risk of hypertension increased in very high altitude (≥3000 m) residents. CONCLUSION: In Derong, despite the relatively low prevalence of metabolic syndrome, hypertension and obesity are more prevalent in adult residents. And metabolic syndrome is more concentrated in township and male residents. These people also have a higher risk of metabolic syndrome. Therefore, it is necessary to develop a national strategy for the prevention and treatment of metabolic syndrome for high-risk population at high altitude in China.

4.
Mol Clin Oncol ; 11(6): 595-598, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31798876

RESUMO

Anlotinib is a multi-target tyrosine kinase inhibitor and has been approved for the treatment of patients with advanced non-small cell lung cancer. The most common adverse events of this treatment include hypertension, fatigue, thyroid-stimulating hormone elevation, hypertriglyceridemia, hand-foot syndrome and hypercholesterolemia. The present study reported the case of a 69-year-old man with squamous cell lung cancer that experienced disease progression following first-line and second-line chemotherapy. Subsequently, anlotinib was administered as a third-line therapy. Following the second cycle of oral targeted therapy, the patient was admitted to the hospital with a one-week history of chest tightnesss, shortness of breath and cough blood-stained sputum and necrosis. Computed tomography scan showed: Bronchopleural fistula (BPF) complicating lung cancer. However, symptoms were not relieved following anti-infective treatment and the patient subsequently died of respiratory failure. To the best of our knowledge, this is the first case of bronchopleural fistula associated with the use of anlotinib in a patient with squamous cell lung cancer.

5.
Bioresour Technol ; 294: 122168, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31569047

RESUMO

In this study, a 20-L spherical explosion vessel, hot plate apparatus, Godbert-Greenwald furnace apparatus, and TG-FTIR were used to investigate the explosion and combustion characteristics of three biomass dusts: dalbergia cochinchinensis (DC), pine sawdust (PS), and cupressus funebris (CF). DC with lower minimum explosible concentration (70-80 g/m3), larger maximum explosion pressure (0.742 MPa) and larger maximum explosion pressure rise rate (80.14 MPa/s) had the greatest explosion hazard in three biomass dusts. The apparent activation energy of DC and CF were lower than that of PS, and the main gas products of three samples included CO2, CO, CH3COOH, and H2O. The volatile matter content of DC characterized by the weight loss and the number of gas products of combustion process was higher than those of PS and CF, showing that the volatile matter content of biomass dust was one of the main factors affecting combustion and explosion hazard.


Assuntos
Poeira , Explosões , Biomassa , Cinética , Madeira
6.
Biol Pharm Bull ; 42(10): 1720-1725, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31378747

RESUMO

Lung cancer is the most common cause of cancer death, approximately 85% of which are non-small cell lung cancer (NSCLC). Here we found that artemether (ART), a natural derivative of artemisinin, significantly inhibits the proliferation of NSCLC cells in a dose- and time-dependent manner. We also demonstrated that high concentration of ART induces apoptosis in NSCLC cells through down-regulating the level of anti-apoptotic protein B-cell lymphoma-2 (Bcl-2), cellular inhibitor of apoptosis protein 1 (cIAP1) and cellular inhibitor of apoptosis protein 2 (cIAP2). While low concentration of ART inhibits the mRNA level of cell cycle related genes including cyclin-dependent kinase 1 (CDK1), cyclin-dependent kinase 2 (CDK2), cyclin-dependent kinase 6 (CDK6), cyclin A2, cyclin B1 and cyclin D1, leading to cell cycle arrest in NSCLC cells. Moreover, we confirmed that low concentration of ART induces DNA double-stranded breaks (DSBs), as well as promoting cellular senescence in NSCLC cells by up-regulating the mRNA and protein level of p16. Taken together, ART represents a promising new anti-NSCLC drug candidate that could attenuate progression of NSCLC cells in a p53-independent manner through inducing apoptosis, cell cycle arrest and promoting cellular senescence.


Assuntos
Apoptose/efeitos dos fármacos , Artemeter/farmacologia , Carcinoma Pulmonar de Células não Pequenas/metabolismo , Pontos de Checagem do Ciclo Celular/efeitos dos fármacos , Senescência Celular/efeitos dos fármacos , Células A549 , Artemeter/uso terapêutico , Carcinoma Pulmonar de Células não Pequenas/tratamento farmacológico , Inibidor p16 de Quinase Dependente de Ciclina/metabolismo , Quinases Ciclina-Dependentes/metabolismo , Ciclinas/metabolismo , Dano ao DNA , Progressão da Doença , Regulação para Baixo , Humanos , Proteínas Inibidoras de Apoptose/metabolismo , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , RNA Mensageiro/metabolismo , Proteína Supressora de Tumor p53/metabolismo , Regulação para Cima
7.
Artigo em Inglês | MEDLINE | ID: mdl-31308853

RESUMO

The traditional Chinese medicine decoction FuFangChangTai (FFCT) has been used in the therapy of colon cancer clinically, yielding alleviated toxicity and enhanced immunity. In our previous study, FFCT exerted its antitumor activity not only by inducing apoptosis but also by activating autophagy to eliminate tumor cells. However, its mechanism is not well understood. The purpose of this study was to investigate the relationship between macrophages activation and FFCT-induced autophagy. Results showed that FFCT could induce autophagy in colon cancer, as demonstrated by increased level of intracellular autophagy marker LC3 II in CT26.WT cells by fluorescence microscope and western blot assay. FFCT also facilitated numbers of vesicular bodies with bilayer membrane in CT26.WT cells, which were indicative of autophagosomes formation. Autophagosomes secreted by FFCT-treated CT26.WT cells can activate M1 type macrophages, accompanied with increased expression of costimulatory molecules CD86 and CD40 on the surface of RAW264.7 cells, and more inflammatory cytokines secretion, such as TNF-α, IL-6, MCP-1, and IL-1ß. mRNA expressions of M2 macrophages markers, such as IL-10, CD206, Arg-1, and FIZZ-1, were downregulated. And this process helps regulate the polarization of macrophages and promote the immune response. These findings support a mechanism of FFCT-induced autophagy and provide novel evidence demonstrating that macrophages are involved in FFCT-induced autophagy progression.

8.
Zhonghua Wei Zhong Bing Ji Jiu Yi Xue ; 31(5): 654-657, 2019 May.
Artigo em Chinês | MEDLINE | ID: mdl-31198159

RESUMO

OBJECTIVE: Acute respiratory distress syndrome (ARDS) is considered to be a pulmonary manifestation of systemic inflammatory response syndrome (SIRS), often occurring as a complication of disease, and worsening the prognosis of patients. In recent years, the incidence of trauma has increased year by year. Severe trauma can lead to SIRS, which is one of the common risk factors of ARDS. The spleen is the largest peripheral immune organ of the body, containing a large number of immune cells and secreting inflammatory factors. The inflammatory factors play an important role in the formation of traumatic ARDS. In recent years, the benefits of treating ARDS by inhibiting spleen-induced inflammatory response have gradually been discovered, providing new ideas for the treatment of ARDS. Therefore, the research status of spleen-mediated inflammatory response in traumatic ARDS is of great significance for the prevention and treatment of traumatic ARDS. This article reports the spleen-mediated systemic inflammatory response, the role of inflammatory mediators in the development of ARDS, and the current state of research on ARDS treatment to explore new approaches to the prevention and treatment of traumatic ARDS.


Assuntos
Síndrome do Desconforto Respiratório do Adulto/etiologia , Baço/metabolismo , Síndrome de Resposta Inflamatória Sistêmica , Ferimentos e Lesões/complicações , Humanos , Síndrome do Desconforto Respiratório do Adulto/terapia
9.
Polymers (Basel) ; 11(5)2019 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-31052422

RESUMO

Dynamic structures containing polymers can behave as thermosets at room temperature while maintaining good mechanical properties, showing good reprocessability, repairability, and recyclability. In this work, alkyl diselenide is effectively used as a dynamic cross-linker for the design of self-healing poly(urea-urethane) elastomers, which show quantitative healing efficiency at room temperature, without the need for any catalysts or external interventions. Due to the combined action of the urea bond and amide bond, the material has better mechanical properties. We also compared the self-healing effect of alkyl diselenide-based polyurethanes and alkyl disulfide-based polyurethanes. The alkyl diselenide has been incorporated into polyurethane networks using a para-substituted amine diphenyl alkyl diselenide. The resulting materials not only exhibit faster self-healing properties than the corresponding disulfide-based materials, but also show the ability to be processed at temperatures as low as 60 °C.

10.
Polymers (Basel) ; 11(2)2019 Feb 13.
Artigo em Inglês | MEDLINE | ID: mdl-30960302

RESUMO

Hyperbranched polycaprolactone with controlled structure was synthesized by reversible addition-fragmentation chain transfer radical ring-opening polymerization along with self-condensed vinyl polymerization (SCVP) of 2-methylene-1,3-dioxepane (MDO). Vinyl 2-[(ethoxycarbonothioyl) sulfanyl] propanoate (ECTVP) was used as polymerizable chain transfer agent. Living polymerization behavior was proved via pseudo linear kinetics, the molecular weight of polymers increasing with conversion and successful chain extension. The structure of polymers was characterized by ¹H NMR spectroscopy, tripe detection gel permeation chromatography, and differential scanning calorimetry. The polymer composition was shown to be able to tune to vary the amount of ester repeat units in the polymer backbone, and hence determine the degree of branching. As expected, the degree of crystallinity was lower and the rate of degradation was faster in cases of increasing the number of branches.

11.
World Neurosurg ; 125: 42-43, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-30731201

RESUMO

BACKGROUND: Infectious pseudoaneurysm of the intracavernous carotid artery is rare, often caused by sphenoid sinusitis or transsphenoidal surgery. Management of intracranial infectious aneurysms (IIAs) remains a controversial topic lacking randomized controlled trials to guide clinical decision making. CASE DESCRIPTION: For this case, we used an antibiotic to treat the patient in the initial stage. Because IIAs enlarge rapidly, endovascular treatment was performed in the emergency department. CONCLUSIONS: Unfortunately, the patient died because the IIA recurred and ruptured. Therefore we speculated that stent-assisted coil placement for the treatment of ruptured infectious pseudoaneurysms of the internal carotid artery may not be safe.


Assuntos
Falso Aneurisma/diagnóstico por imagem , Falso Aneurisma/cirurgia , Artéria Carótida Interna/diagnóstico por imagem , Artéria Carótida Interna/cirurgia , Procedimentos Endovasculares/métodos , Falso Aneurisma/microbiologia , Angiografia Digital/métodos , Artéria Carótida Interna/microbiologia , Evolução Fatal , Humanos , Masculino , Pessoa de Meia-Idade , Resultado do Tratamento
12.
Sci Rep ; 8(1): 15123, 2018 10 11.
Artigo em Inglês | MEDLINE | ID: mdl-30310107

RESUMO

Systemic inflammatory response has been implicated as a contributor to the onset of febrile seizures (FS). The four novel indices of the inflammatory response such as, neutrophil-to-lymphocyte ratio (NLR), mean platelet volume (MPV), platelet count (PLT) ratio and red blood cell distribution width (RDW) have been investigated in FS susceptibility and FS types (simple febrile seizure and complex febrile seizure). However, the potential role of these inflammatory markers and MPV/PLT ratio (MPR) in Chinese children with FS has yet to be fully determined. This study investigated the relevance of NLR, MPV, PLT, MPR and RDW in febrile children with and without seizures. 249 children with FS and 249 age matched controls were included in this study. NLR and MPR were calculated from complete blood cell counts prior to therapy. Differences in age, gender and these inflammatory markers between the FS group and the control group were evaluated using the chi-square test, t-test or logistic regression analysis. Receiver Operating Characteristic (ROC) curve was used to determine the optimal cut-off value of NLR and MPR for FS risk. Interactions between NLR and MPR on the additive scale were calculated by using the relative excess risk due to interaction (RERI), the proportion attributable to interaction (AP), and the synergy index (S). It has been shown that the elevated NLR and MPR levels were associated with increased risk of FS. The optimal cut-off values of NLR and MPR for FS risk were 1.13 and 0.0335 with an area under the curve (AUC) of 0.768 and 0.689, respectively. Additionally, a significant synergistic interaction between NLR and MPR was found on an additive scale. The mean levels of MPV were lower and NLR levels were higher in complex febrile seizure (CFS) than simple febrile seizure (SFS), and the differences were statistically significant. ROC analysis showed that the optimal cut-off value for NLR was 2.549 with 65.9% sensitivity and 57.5% specificity. However, no statistically significant differences were found regarding average values of MPR and RDW between CFS and SFS. In conclusion, elevated NLR and MPR add evidence to the implication of white cells subsets in FS risk, and our results confirmed that NLR is an independent, albeit limited, predictor in differentiating between CFS and SFS. Moreover, NLR and MPR may have a synergistic effect that can influence the occurrence of FS.


Assuntos
Contagem de Leucócitos , Linfócitos , Volume Plaquetário Médio , Neutrófilos , Contagem de Plaquetas , Convulsões Febris/sangue , Área Sob a Curva , Biomarcadores , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Masculino , Prognóstico , Curva ROC , Fatores de Risco , Convulsões Febris/diagnóstico , Convulsões Febris/etiologia
13.
Waste Manag ; 77: 131-139, 2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30008402

RESUMO

In order to enhance the immobilization of heavy metals in the bio-char during microwave pyrolysis, the immobilization efficiency and mechanism of heavy metals in the microwave pyrolysis of sludge with different alkaline catalysts were explored. Results showed that the leaching concentrations of heavy metals reduced greatly after pyrolysis, which were lower when catalyzed by CaO than those of Fe2O3. CaO was more favorable for the immobilization of Cr, Cu, Zn, Pb and Ni while Fe2O3 was more favorable for Cd. Different species distributions of heavy metals in the bio-char affected the leaching concentrations. Adding catalyst could significantly reduce the ecological risks of heavy metals in the bio-char, and CaO (RI = 15.17-20.43) had a better performance than Fe2O3 (RI = 16.88-21.79). When catalyzed by CaO, the formation of pores and co-crystal compounds in the bio-char determined the immobilization efficiencies of heavy metals.


Assuntos
Metais Pesados , Micro-Ondas , Esgotos/química , Catálise , Compostos Férricos
14.
Immunol Lett ; 202: 1-7, 2018 10.
Artigo em Inglês | MEDLINE | ID: mdl-30053453

RESUMO

Microglia polarization plays a crucial role in initiating brain inflammatory injury after intracerebral hemorrhage (ICH). Casein Kinase 2 Interacting Protein 1(CKIP-1) has been identified as a transcriptional molecular to manipulate microglia polarization. MiRNAs regulate gene expression and microglia polarization. In the experiment, CKIP-1 has been predicted as a target gene of let-7a. Let-7a, CKIP-1 and downstream proinflammatory mediator production of ICH mice were analyzed. In addition, inflammation, brain edema, and neurological functions in ICH mice were also assessed. Furthermore, let-7a mimic or inhibitors was administrated to study the potential role to manipulate microglia polarization after ICH. We reported that let-7a levels decreased but CKIP-1 levels increased after ICH. Using a dual-luciferase reporter assay, it was demonstrated that CKIP-1 was the target gene of let-7a. Let-7a overexpression decreased the protein levels of CKIP-1 and inhibition of let-7a increased the protein levels of CKIP-1. In addition, our results indicate that let-7a could inhibit expression of proinflammatory cytokines, reduce brain edema, and improve neurological functions in ICH mice. The study indicated that CKIP-1 was a target gene of let-7a and that let-7a regulated microglia M2 polarization by targeting CKIP-1 following ICH.


Assuntos
Proteínas de Transporte/genética , Macrófagos/patologia , MicroRNAs/genética , Microglia/patologia , Animais , Hemorragia Cerebral/genética , Hemorragia Cerebral/patologia , Citocinas/genética , Modelos Animais de Doenças , Expressão Gênica/genética , Regulação da Expressão Gênica/genética , Inflamação/genética , Inflamação/patologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL
15.
Mol Immunol ; 101: 38-45, 2018 09.
Artigo em Inglês | MEDLINE | ID: mdl-29859495

RESUMO

IL-17A contributes to the initiation of inflammation following intracerebral hemorrhage (ICH). Endoplasmic reticulum (ER) stress acts on protein folding and contributes to inflammatory diseases. The role of IL-17A in the regulation of ER stress following ICH has not been well characterized. In this study, macrophages were stimulated with IL-17A, and then, ER stress and downstream pro-inflammatory factors were measured in vitro. In addition, brain edema and brain injury in ICH mice were assessed in vivo. We demonstrated that IL-17A induced ER stress in macrophages and thus promoted inflammation in vitro. Conversely, IL-17A inhibition attenuated ER stress and neuroinflammation. Furthermore, ERK 1/2 and p38 MAPK pathways mediated IL-17A-induced ER stress in macrophages. We also showed that IL-17A inhibition significantly attenuated ER stress and brain injury in ICH mice. In conclusion, our results demonstrate that IL 17A increases ER stress in macrophages and represents a novel mechanism in ICH.


Assuntos
Hemorragia Cerebral/patologia , Progressão da Doença , Estresse do Retículo Endoplasmático , Inflamação/patologia , Interleucina-17/metabolismo , Macrófagos/patologia , Animais , MAP Quinases Reguladas por Sinal Extracelular/metabolismo , Ativação de Macrófagos , Macrófagos/metabolismo , Macrófagos/ultraestrutura , Masculino , Camundongos Endogâmicos C57BL , Neurônios/metabolismo , Neurônios/patologia
16.
Bioresour Technol ; 259: 286-293, 2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-29573607

RESUMO

In order to investigate the heterotrophic nitrification and aerobic denitrification ability of Acinetobacter harbinensis HITLi7T at 2 °C, both the growth parameters and substrates utilization characteristics were tested and appropriated kinetic models were obtained in this study. Under the initial concentration of 5 mg/L, the maximum NH4+-N and NO3--N degradation rates were 0.076 mg NH4+-N/L/h and 0.029 mg NO3--N/L/h, respectively. At the simultaneous presence of 2.5 mg/L NH4+-N and NO3--N, the maximum nitrate removal rate increased to 0.054 mg NO3--N/L/h (1.86 folds), while a slight decrease was observed in NH4+-N removal. Two double-substrate models, Contois-Contois (1) for NH4+-N and TOC, Monod-Contois (2) for NO3--N and TOC matched well with the experimental data. The kinetic parameters were determined as µmax1 = 0.095 h-1, BA1 = 0.012 mg/L, BT1 = 0.784 g TOC/g biomass (R12 = 0.9997), and µmax2 = 0.032 h-1, KN2 = 0.375 mg/L, BT2 = 1.108 g TOC/g biomass (R22 = 0.9731) by multiple regression equation.


Assuntos
Acinetobacter , Desnitrificação , Aerobiose , Processos Heterotróficos , Nitrificação , Nitrogênio
17.
Diabetes Res Clin Pract ; 138: 246-252, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29408704

RESUMO

OBJECTIVE: Early short-term intensive insulin therapy in newly diagnosed type 2 diabetes patients shows benefit in glycemic control and ß-cell function. Glucagon-like peptide-1 (GLP-1) plays an important role in glucose metabolism and development of type 2 diabetes. We did a study to observe the changes of GLP-1 and ß-cell function after short-term continuous subcutaneous insulin infusion (CSII) treatment. METHODS: A total of 66 subjects were enrolled, including 30 normal glucose tolerance controls (NGT) and 36 patients with newly diagnosed type 2 diabetes between October 2015 and July 2016. Fasting plasma glucose (FPG), insulin, and GLP-1 were measured in each subject. The patients underwent CSII treatment for 2 weeks, and then FBG, insulin, and GLP-1 were measured. HOMA-IR and HOMA-B were then calculated. RESULTS: All patients achieved target glycemic control in two weeks. HOMA-IR and HOMA-B improved significantly after intensive interventions (p < 0.05). The GLP-1 concentration increased significantly in patients after treatment (p < 0.05). When grouped according to bodyweight and age in all patients, the HOMA-IR changed significantly in overweight and old age subgroups, the HOMA-B increased significantly in normal weight, overweight and middle age subgroups, and the GLP-1 concentration also increased significantly in overweight and middle age subgroups respectively (p < 0.05). CONCLUSION: Short-term CSII treatment can obtain glycemic control target and recover ß-cell function and GLP-1 secretion in newly diagnosed type 2 diabetes patients. The overweight and middle-aged patients may get more benefit from this treatment.


Assuntos
Diabetes Mellitus Tipo 2/sangue , Diabetes Mellitus Tipo 2/tratamento farmacológico , Peptídeo 1 Semelhante ao Glucagon/sangue , Insulina/administração & dosagem , Adulto , Idoso , Glicemia/metabolismo , Diabetes Mellitus Tipo 2/diagnóstico , Esquema de Medicação , Sistemas de Liberação de Medicamentos , Jejum/sangue , Feminino , Humanos , Infusões Subcutâneas , Masculino , Pessoa de Meia-Idade , Fatores de Tempo
18.
Mol Cell Endocrinol ; 470: 259-268, 2018 07 15.
Artigo em Inglês | MEDLINE | ID: mdl-29138023

RESUMO

It has been reported that inflammation is closely related with cardiac hypertrophy. Some inflammatory cytokines such as tumor necrosis factor-α, interleukin-1ß, and interleukin-6 directly induce cardiac hypertrophy, which is associated with the activation of nuclear factorkappa B (NF-κB). Thus, NF-κB is an attractive target for cardiac hypertrophy. In the present study, oleanonic acid inhibited the elevation of transcriptional activity of NF-κB and reduced the mRNA expressions of hypertrophic genes such as atrial natriuretic factor (ANF) and brain natriuretic peptide (BNP) in a concentration-dependent manner in phenylephrine (PE)-treated cardiomyocytes. Furthermore, we found that oleanonic acid inhibited the phosphorylation of protein kinase C ζ (PKCζ) at Thr410 site and then reduced the activation of NF-κB using gain- and loss-of-function approaches in PE-treated cardiomyocytes. In vivo, similar results were observed in abdominal aortic constriction (AAC) rats that were intragastrically administered with oleanonic acid, and the pathological changes accompanying cardiac hypertrophy were relieved. In conclusion, oleanonic acid can effectively ameliorate cardiac hypertrophy by inhibiting PKCζ-NF-κB signaling pathway.


Assuntos
Cardiomegalia/tratamento farmacológico , Cardiomegalia/metabolismo , NF-kappa B/metabolismo , Pressão , Proteína Quinase C/metabolismo , Transdução de Sinais , Triterpenos/uso terapêutico , Angiotensina II , Animais , Aorta/patologia , Cardiomegalia/patologia , Constrição Patológica , Técnicas de Silenciamento de Genes , Masculino , Miócitos Cardíacos/efeitos dos fármacos , Miócitos Cardíacos/metabolismo , Fenilefrina , Fosforilação/efeitos dos fármacos , Ratos Sprague-Dawley , Fator de Transcrição RelA/metabolismo , Transcrição Genética/efeitos dos fármacos , Triterpenos/química , Triterpenos/farmacologia
19.
Neural Regen Res ; 12(9): 1472-1478, 2017 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-29089993

RESUMO

Herb-partitioned moxibustion can effectively mitigate visceral pain, a major symptom in inflammatory bowel disease, but the analgesic mechanism is still unclear. Moreover, extracellular signal-regulated kinase, substance P, and neurokinin-1 are involved in formation of central hyperalgesia. Thus, we postulated that the analgesic effect of herb-partitioned moxibustion may be associated with these factors. Accordingly, in this study, we established an inflammatory bowel disease visceral pain model in rat by enema with a mixed solution of 5% trinitrobenzenesulfonic acid and 50% ethanol. Bilateral Tianshu (ST25) and Qihai (CV6) points were selected for herb-partitioned moxibustion. Our results showed that herb-partitioned moxibustion improved visceral pain and down-regulated extracellular signal-regulated kinase, substance P, and neurokinin-1 protein and mRNA expression in dorsal root ganglia. These results indicate that down-regulation of extracellular signal-regulated kinase, substance P, and neurokinin-1 protein and mRNA may be a central mechanism for the analgesic effect of herb-partitioned moxibustion.

20.
Bioresour Technol ; 244(Pt 1): 800-809, 2017 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-28830043

RESUMO

In order to make a better understanding of the characteristics of N2O emission in A/O wastewater treatment plant, full-scale and pilot-scale experiments were carried out and a back propagation artificial neural network model based on the experimental data was constructed to make a precise prediction of N2O emission. Results showed that, N2O flux from different units followed a descending order: aerated grit tank>oxic zone≫anoxic zone>final clarifier>primary clarifier, but 99.4% of the total emission of N2O (1.60% of N-load) was monitored from the oxic zone due to its big surface area. A proper DO control could reduce N2O emission down to 0.21% of N-load in A/O process, and a two-hidden-layers back propagation model with an optimized structure of 4:3:9:1 could achieve a good simulation of N2O emission, which provided a new method for the prediction of N2O emission during wastewater treatment.


Assuntos
Águas Residuárias , Reatores Biológicos , Óxido Nitroso
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