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1.
Artigo em Inglês | MEDLINE | ID: mdl-31619447

RESUMO

OBJECTIVE: To describe the detailed clinical characteristics, immunotherapy, and long-term outcomes of patients with anti-NMDA receptor (NMDAR) encephalitis in China. METHODS: A single-center, prospective study. Patients who met the diagnostic criteria were enrolled from 2011 to 2017 and followed up. The clinical features, treatment, and long-term outcomes were collected prospectively. Factors affecting the long-term prognosis were analyzed. RESULTS: The study included 220 patients. The most common clinical presentations were psychosis (82.7%) and seizures (80.9%). Of the patients, 19.5% had an underlying neoplasm; of which ovarian teratoma was 100% of tumors in females and only one male had lung cancer. Most patients (99.5%) received first-line therapy (glucocorticoids, IV immunoglobulin, or plasmapheresis alone or combined), and only 7.3% received second-line immunotherapy (rituximab, cyclophosphamide alone, or combined). Long-term immunotherapy (mycophenolate mofetil or azathioprine >1 year) was administered to 53.2% of patients. During the first 12 months, 207 (94.1%) patients experienced improvement, and 5 (2.3%) died, whereas 38 (17.3%) experienced relapses. At 12-month follow-up, 92.7% had favorable clinical outcomes (modified Rankin Scale score ≤2). CONCLUSIONS: Patients in China present with psychosis and seizure frequently but have a low percentage of underlying neoplasms. Re-enforced first-line immunotherapy is effective in managing anti-NMDAR encephalitis in the acute phase. Although relapse is relatively common, with combined first-line and long-term immunotherapy, most patients reached favorable outcomes.

2.
Eur J Cancer Prev ; 29(1): 36-41, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30913093

RESUMO

Metabolic syndrome (MetS) can increase the risk of prostate cancer. Prostate-specific antigen (PSA) is the marker for prostate cancer puncture screening. The aim of our study was to investigate the association between MetS and its components with PSA levels. Data were obtained from 482 943 healthy men who underwent routine health check-ups from January 2010 to December 2017. We used linear regression analysis to evaluate the effects of MetS and its components on PSA levels. To explore the cumulative effect of MetS components, analysis of variance trend analysis was carried out. The PSA levels in the group with MetS were significantly lower than those without MetS (P = 0.001). In the multivariate regression model, age (P < 0.001) and hypertension (P < 0.001) were correlated positively with PSA levels; nevertheless, obesity (P < 0.001), hypertriglyceridemia (P < 0.001), hyperglycemia (P < 0.001), and low high-density lipoprotein cholesterol level (P < 0.001) had a negative correlation. In addition, after adjustment for age, increasing sums of positive MetS components were associated with a linear decrease in PSA levels (P<0.001). In conclusion, MetS, obesity, hypertriglyceridemia, hyperglycemia, and low high-density lipoprotein cholesterol levels are associated with decreased PSA levels. For patients with PSA levels at the critical value of prostate puncture, the effect of these diseases in reducing PSA levels should be taken into account.

3.
J Cell Physiol ; 235(1): 232-244, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31190343

RESUMO

Diabetes is often accompanied by dysfunction of salivary glands. However, the molecular mechanism remains unclear. The mechanisms that underlie diabetic hyposalivation were studied by db/db mice and SMG-C6 cells. We found morphological changes and decreased stimulated salivary flow rates of the submandibular gland (SMG) in diabetic mice. We observed structural changes and dysfunction of mitochondria. More mitophagosomes and higher expression of autophagy-related proteins were detected. Increased levels of proteins PINK1 and Parkin indicate that PINK1/Parkin-mediated mitophagy was activated in diabetic SMG. Consistently, high glucose (HG) induced mitochondrial dysfunction and PINK1/Parkin-mediated mitophagy in cultivated SMG-C6 cells. HG also increased reactive oxygen species (ROS) and lessened activation of antioxidants in SMG-C6 cells. In addition, HG lowered ERK1/2 phosphorylation and HG-induced mitophagy was decreased after ERK1/2 was activated by LM22B-10. Altogether, these data suggest that ROS played a crucial role in diabetes-induced mitochondrial dysfunction and PINK1/Parkin-mediated mitophagy and ERK1/2 was required in HG-induced mitophagy in SMG.

4.
Clin EEG Neurosci ; 51(1): 51-60, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31450965

RESUMO

Objective. Electroencephalogram (EEG) is a sensitive method for evaluation of anti-N-methyl-d-aspartate receptor (anti-NMDAR) encephalitis. The aim was to investigate the EEG and clinical features and correlations in patients with anti-NMDAR encephalitis. Methods. Anti-NMDAR encephalitis patients (n = 98) with at least 1 EEG recording in our hospital were recruited between January 2012 and October 2017. We reviewed and analyzed the EEG and clinical data. Results. In our cohorts, 79 patients underwent EEG in the disease acute stage, and 39 in the recovery stage. Of the 79 patients, 70 (88.6%) EEG recordings in acute period were abnormal. Symptoms, including consciousness, movement disorder, coma, were correlated to the degree of EEG abnormalities (P < .05). The patients with more severe EEG abnormalities also had longer hospitalized and intensive care unit stay time (P < .05). We found that the EEG pattern of abnormal occipital alpha rhythm had a correlation with the clinical severity, and the Spearman coefficient was 0.448 (P = .000). Neither delta activities distribution nor prevalence showed correlations with clinical severity in acute stage. However, delta activities significantly decreased in the disease recovery stage. The other findings of EEG records were extreme delta brush (7cases, 8.9%), excess delta activities (diffuse slowing 30 cases, 38.0%), and epileptiform discharge (10 cases, 14.3%). Conclusion. This is the largest study of EEG recording in patients with anti-NMDAR encephalitis patients in China. EEG abnormalities, particularly occipital alpha rhythm, are correlated with clinical severity. EEG is useful for diagnosis and monitoring of treatment response in conjunction with clinical improvement.

5.
Appl Radiat Isot ; 155: 108948, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31655352

RESUMO

A measurement method of 85Kr using an internal gas proportional counter (IGPC) is presented in this study. The operation conditions of the IGPC were determined and optimized, including the operating voltage, pressure, sample volume, interference from other gas components such as nitrogen or air, and mitigation of the memory effect. The IGPC was calibrated using certified standards, and the detection efficiency was approximately 58% for typical samples. A lower limit of detection of approximately 0.11 MBq/m3(Kr) was achieved after counting for 5 h with 1 mL pure Kr, corresponding to the atmospheric activity concentration of 0.18 Bq/m3 (air). It was shown that the IGPC could be used effectively for measuring 85Kr.

6.
Carbohydr Polym ; 228: 115398, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31635734

RESUMO

In this study, amphiphilic conjugates were synthesized by conjugating curcumin (CUR) to a food-derived hydrophilic hydroxyethyl starch (HES) via an acid-labile ester linker. The self-assembly of the conjugates formed uniform micellar nanoparticles (HES-CUR NPs) with a desirable drug loading efficiency, excellent colloidal and storage stability, as well as acid-responsive release manner. Besides, the formation of the nanoparticles increased the solubility of CUR to thousands times higher than free CUR, and effectively protected the loaded CUR from degradation upon exposure to UV light and high temperature. In vitro cytotoxicity assay and radical scavenging experiments demonstrated that the HES-CUR NPs significantly improved the cytocompatibility, anticancer and antioxidant activity of CUR due to the enhanced solubility, stability, and bioavailability. The HES-CUR NPs reported herein have a great potential in developing functional food or pharmaceutical formulations for preventing or treating various diseases such as inflammatory diseases and cancer.

7.
J Nanosci Nanotechnol ; 20(4): 2104-2113, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-31492218

RESUMO

In this work, stable superparamagnetic core-shell Fe2B@SiO2 nanoparticles were prepared by "one pot" chemical reduction of ferric ion salts with NaBH4 and silica shell coating in the presence of citric acid stabilizer. The X-ray (XRD) and selection of electron diffraction (SAED) revealed that Fe2B@SiO2 nanoparticles were chiefly composed of silica shell and Fe2B with a small amount of α-Fe. Then, graphene oxide (GO) was assembled onto core-shell Fe2B@SiO2 nanoparticles for Epirubicin (EPI) loading and releasing, and the adsorption isotherm of EPI on Fe2B@SiO2@GO was analyzed. The results showed, the adsorption process includes two steps, due to the π-π stacking effect of EPI on the Fe2B@SiO2@GO. The EPI loading efficiency of the magnetic nanoparticles was as high as 90%, while the EPI-loaded core-shell Fe2B@SiO2 nanoparticles showed an obvious pH controlled drug release behavior, and showed lower cumulative release rate when pH is 7.4 than that when pH is 5.7. The prepared nanoparticles demonstrated potential application in killing tumor cells while minimizing the toxicity to normal tissues.

8.
Int J Mol Med ; 2019 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-31789424

RESUMO

Oxidative and inflammatory damage has been suggested to play important roles in the pathogenesis of skin photoaging. Andrographolide sodium bisulfate (ASB) is a soluble derivative of andrographolide and has known antioxidant and anti­inflammatory properties. In the present study, cellular experiments were designed to investigate the molecular mechanisms underlying the effect of ASB in relieving ultraviolet (UV)­induced photo­damage. Following ASB pretreatment and UV irradiation, the apoptosis and necrosis of HaCaT cells were investigated by Hoechst 33342/propidium iodide staining. Reactive oxygen species (ROS) production was investigated using a DCFH­DA fluorescence probe. Furthermore, the protein expression levels of p65, NF­κB inhibitor­α, nuclear factor E2­related factor 2 (Nrf2) and kelch­like ECH­associated protein 1 (keap1) were measured via western blotting and immunofluorescence analyses. Furthermore, NF­κB­mediated cytokines were assessed by ELISA, and Nrf2­mediated genes were detected by reverse transcription­quantitative PCR. Pretreatment with ASB markedly increased cell viability, decreased cell apoptosis and decreased UV­induced excess ROS levels. In addition, ASB activated the production of Nrf2 and increased the mRNA expression levels of glutamate­cysteine ligase catalytic subunit and NAD(P)H quinone oxidoreductase 1, while ASB downregulated the protein expression of p65 and decreased the production of interleukin (IL)­1ß, IL­6 and tumor necrosis factor­α. These results suggested that ASB attenuates UV­induced photo­damage by activating the keap1/Nrf2 pathway and downregulating the NF­κB pathway in HaCaT keratinocytes.

9.
Artigo em Inglês | MEDLINE | ID: mdl-31796403

RESUMO

As more and more satellites are launched into the space, dynamic estimation of spin satellites has become a critical component of the space situation awareness application. Some explored studies using exterior measurements from different sensors such as optical device and inverse synthetic aperture radar (ISAR) to estimate dynamic parameters of spin satellites. As a single sensor normally provides two-dimensional observation, three-dimensional estimations resulting from these algorithms are strictly related to the prior knowledge of targets characteristics. As a result, it is difficult to expand these methods to other satellites. In order to support the dynamic estimation of most spin satellites, this paper presents a novel dynamic estimation approach which employs synchronized optical-and-radar images. The optical-and-radar fusion strategy has demonstrated its superiority in image analysis field, and breaks down the dynamic estimation of spin satellites into two sub-problems: target attitude estimation and spin parameters estimation. In this work, the proposed algorithm deduces two explicit expressions of target dynamic parameters under the imaging projection model of the joint optical-and-radar observation. Through the particle swarm optimization (PSO), target dynamic parameters are determined in two stages. This paper presents some experiments illustrating the feasibility of the proposed method and subsequent conclusions, which reflect advantages of the joint optical-and-radar observation mode in image interpretation.

10.
Environ Microbiol ; 2019 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-31797523

RESUMO

Rice false smut has emerged as a serious grain disease in rice production worldwide. The disease is characterized by the transformation of individual rice florets into false smut balls, which is caused by the fungal pathogen Ustilaginoidea virens. To date, little is known about the host factors required for false smut ball formation by U. virens. In this study, we identified histological determinants for the formation of false smut balls by inoculating U. virens into rice floral mutants defective with respect to individual floral parts. The results showed that U. virens could form mature false smut balls in rice floral mutants with defective pistils, but failed to develop false smut balls in the superwoman mutant lacking stamens, identifying that U. virens requires rice stamens to complete its infection cycle. Comparative transcriptome analysis indicated a list of candidate host genes that may facilitate nutrient acquisition by U. virens from the rice stamens, such as SWEET11, SWEET14 and SUT5, and genes involved in the biosynthesis of trehalose and raffinose family sugars. These data pinpoint rice stamens as the key target organ of U. virens infection, and provide a valuable starting point for dissecting the molecular mechanism of false smut ball formation. This article is protected by copyright. All rights reserved.

11.
Artigo em Inglês | MEDLINE | ID: mdl-31802419

RESUMO

Regulatory T cells (Tregs), which secrete transforming growth factor (TGF)-ß and interleukin (IL)-10, have essential role in anti-inflammatory and neurotrophic functions. Herein, we explore the neuroprotection of Tregs in Parkinson's disease (PD) by adoptive transfer of Tregs. Tregs, isolated by magnetic sorting, were activated in vitro and then were adoptively transferred to 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP)-treated mice. Neuroinflammation, dopaminergic neuronal loss and behavioral changes of PD mice were evaluated. Live cell imaging system detected a dynamic contact of Tregs with MN9D cells that were stained with CD45 and galectin-1, respectively. Tregs prevented MPTP-induced dopaminergic neuronal loss, behavioral changes, and attenuated the inflammatory reaction in the brain. When blockade the LFA-1 activity in Tregs or the ICAM-1 activity in endothelial cells, the percentage of Tregs in substantia nigra (SN) decreased. CD45 and galectin-1 were expressed by Tregs and MN9D cells, respectively. CD45-labeled Tregs dynamically contacted with galectin-1-labeled MN9D cells. Inhibiting CD45 in Tregs impaired the ability of Tregs to protect dopaminergic neurons against MPP+ toxicity. Similarly, galectin-1 knockdown in MN9D cells reduced the ability of Tregs neuroprotection. Adoptive transfer of Tregs protects dopaminergic neurons in PD mice by a cell-to-cell contact mechanism underlying CD45-galectin-1 interaction. Graphical Abstract.

12.
Asian J Surg ; 2019 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-31718878

RESUMO

OBJECTIVE: To evaluate the optimal timing of surgery after short-course radiation therapy and to compare the efficacy of short-course radiotherapy (SCRT) and long-course radiotherapy (LCRT) in patients with rectal cancer, a systematic review with network analysis was conducted. METHODS: A systematic literature search of the Cochrane Central Register of Controlled Trials, Embase, and MEDLINE (PubMed) was conducted to identify papers published before June 4, 2018, without language or publication date restrictions. The use of surface under the cumulative ranking curve (SUCRA) within a network meta-analysis framework provided a numerical presentation of the overall ranking, thus providing a ranking of treatment options from which patients can choose from. Within the primary search that yielded 1435 studies, 11 relevant randomized trials were identified. RESULTS: No statistically significant difference was found in overall mortality and metastasis between short-course radiotherapy with early surgery (SCES), short-course radiotherapy with delayed surgery (SCDS), and long-course radiotherapy with delayed surgery (LCDS). For overall metastasis, using SUCRA analysis, SCES, LCDS, and SCDS respectively had the highest to lowest SUCRA rank, respectively. Thus, SCES was the most likely to be effective, followed by LCDS and SCDS. CONCLUSION: Regarding the optimal timing of surgery for patients with advanced rectal cancer undergoing short-course radiation therapy, SCES is recommended as the optimal choice according to the available evidence and considering the control of future metastasis.

13.
Cancer Commun (Lond) ; 39(1): 69, 2019 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-31699150

RESUMO

BACKGROUND: Gefitinib, as the first epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKI) approved for the treatment of advanced non-small cell lung cancer (NSCLC), has been proved to significantly improve the progression-free survival (PFS) in the first-line setting but suffers from resistance 7-10 months after treatment initiation. Apatinib (YN968D1), a potent vascular endothelial growth factor receptor (VEGFR) 2-TKI, specifically binds to VEGFR2 and leads to anti-angiogenetic and anti-neoplastic effect. Concurrent inhibition of VEGFR and EGFR pathways represents a rational approach to improve treatment responses and delay the onset of treatment resistance in EGFR-mutant NSCLC. This ACTIVE study aims to assess the combination of apatinib and gefitinib as a new treatment approach for EGFR-mutant NSCLC as a first-line setting. METHODS: This multicenter, randomized, double-blind, placebo-controlled phase III study (NCT02824458) has been designed to assess the efficacy and safety of apatinib or placebo combined with gefitinib as a first-line treatment for patients with EGFR-mutant advanced NSCLC. A total of 310 patients with EGFR-mutation (19del or 21L858R), pathological stage IIIB to IV non-squamous NSCLC were to be enrolled. The primary endpoint is investigator assessment of PFS, and the secondary endpoints include independent radiological central (IRC)-confirmed PFS, overall survival (OS), objective response rate (ORR), disease control rate (DCR), time to progressive disease (TTPD), duration of response (DoR), quality of life (QoL) and safety. The patients are randomized in a 1:1 ratio to receive gefitinib (250 mg, p.o. q.d.) plus apatinib (500 mg, p.o. q.d.) or gefitinib plus placebo, given until disease progression or intolerable adverse events. Exploratory biomarker analysis will be performed. This study is being conducted across China and comprises of 30 participating centers. Enrollment commenced in August 2017 and finished in December 2018, most of the patients are in the follow-up period. ANTICIPATED OUTCOMES AND SIGNIFICANCE: The present study will be the first to evaluate the efficacy and safety profile of the combination of apatinib plus gefitinib as a first-line therapy for patients with EGFR-positive advanced non-squamous NSCLC. Importantly, this trial will provide comprehensive evidence on the treatment of EGFR-TKIs combined with antiangiogenic therapy. Trial registration Clinicaltrials.gov NCT02824458. Registered 23 June 2016.

14.
Artigo em Inglês | MEDLINE | ID: mdl-31675328

RESUMO

The low-rank approximation problem has recently attracted wide concern due to its excellent performance in real-world applications such as image restoration, traffic monitoring, and face recognition. Compared with the classic nuclear norm, the Schatten-p norm is stated to be a closer approximation to restrain the singular values for practical applications in the real world. However, Schatten-p norm minimization is a challenging non-convex, non-smooth, and non-Lipschitz problem. In this paper, inspired by the reweighted ℓ1 and ℓ2 norm for compressive sensing, the generalized iterative reweighted nuclear norm (GIRNN) and the generalized iterative reweighted Frobenius norm (GIRFN) algorithms are proposed to approximate Schatten-p norm minimization. By involving the proposed algorithms, the problem becomes more tractable and the closed solutions are derived from the iteratively reweighted subproblems. In addition, we prove that both proposed algorithms converge at a linear rate to a bounded optimum. Numerical experiments for the practical matrix completion (MC), robust principal component analysis (RPCA), and image decomposition problems are illustrated to validate the superior performance of both algorithms over some common state-of-the-art methods.

15.
J Gen Intern Med ; 2019 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-31745855

RESUMO

BACKGROUND: Computerized cognitive behavioral therapy (cCBT) can improve mental health outcomes in White populations; however, it is unknown whether racial and ethnic minority populations receive clinical benefits from cCBT. OBJECTIVE: To study race differences in the impact of cCBT use on mental health outcomes among White and African American primary care patients. DESIGN: Secondary analysis of a three-arm randomized controlled clinical trial. PARTICIPANTS: Primary care physicians (PCPs) referred 2,884 patients aged 18-75; 954 met eligibility criteria (including elevated mood and/or anxiety symptoms indicated as a score ≥ 10 on Patient Health Questionnaire or Generalized Anxiety Disorder scale); 704 were randomized in 3:3:1 ratio to receive either (1) the cCBT program (cCBT-only), (2) cCBT plus access to an Internet Support Group (cCBT+ISG), or (3) their PCP's usual care (UC). After exclusions, this study analyzed 689 patients: 590 receiving cCBT, in the combined cCBT-only and cCBT+ISG groups (91 African American, 499 White), and 99 receiving UC (22 African American, 77 White). INTERVENTION(S): We used the Beating the Blues cCBT program that consisted of eight 50-min Internet-delivered interactive sessions and "homework" assignments to complete between weekly sessions. College graduate-level care coaches provided six months of remote support. MAIN MEASURE(S): After prior analyses demonstrated no effect of the ISG program, we combined the cCBT-only and cCBT+ISG groups (cCBT) to compare to UC at 6-month follow-up. Controlling for sociodemographic factors, baseline symptoms, and treatment arm, we examined race differences for impact of cCBT versus UC on the mental health-related quality-of-life (Short-form 12 Health Survey), and Patient-Reported Outcomes Measurement Information System (PROMIS) anxiety, and depression. RESULTS: Compared to UC, cCBT had no effect on quality of life (d = 0.10; p = 0.40), depression (d = - 0.19; p = 0.10), or anxiety (d = - 0.16; p = 0.18) for Whites. However, for African American patients, cCBT was associated with significant 6-month decrease in depression (d = - 0.47, p < 0.01) and anxiety scores (d = - 0.54, p < 0.01). CONCLUSIONS: cCBT may be an efficient and scalable first step to eliminating disparities in mental health care. TRIAL REGISTRATION: Clinicaltrials.gov Identifier: NCT01482806. https://www.clinicaltrials.gov/ct2/show/NCT01482806?term=rollman&rank=4.

16.
Artif Organs ; 2019 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-31736099

RESUMO

Engineering of functional vascularized pancreatic tissues offers an alternative way to solve the perpetual shortage of organs for transplantation. However, revascularization remains a major bottleneck in biological engineering, which limited the further clinical applications of this strategy. In this study, an efficient approach for enhancing re-endothelialization of rat decellularized pancreatic scaffolds (DPS) was presented, by conjugating with GRGDSPC peptide to maximize coverage of the vessel walls with human umbilical vein endothelial cells (HUVEC). Firstly, pancreas was perfused with 1% Triton X-100 and 0.1% ammonium hydroxide to remove the cellular components. Subsequently, covalently coupled GRGDSPC to the vasculature of DPS and re-seeded HUVEC via perfusion of the portal vein in the bioreactor. After the re-endothelialized scaffolds were created, in vitro and in vivo experiment was taken to evaluate the angiogenesis. Our results demonstrated that, GRGDSPC-conjugated scaffolds could support the survival and accelerated the proliferation of HUVEC, angiogenesis was also significantly improved than untreated scaffolds. In conclusion, GRGDSPC-conjugated scaffolds showed great potential for the generation of functional bioengineered pancreatic tissue suitable for long-term transplantation.

17.
J Cell Mol Med ; 2019 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-31733095

RESUMO

Circular RNA FOXO3 (CircFOXO3, also termed as Hsa_circ_0006404) is derived from exon 2 of forkhead box O3 (FOXO3) gene, and abnormal expression is shown in different diseases. However, whether circFOXO3 plays important roles in tumorigenesis and progression of prostate cancer (PCa) remains unclear. In this study, we found that circFOXO3 was up-regulated in both PCa tissues and serum samples. Moreover, circFOXO3 was positively correlated with the Gleason score in PCa samples. CircFOXO3 was observed to be up-regulated in Gleason score > 6 PCa samples compared with Gleason score = 6 PCa samples. Knock-down circFOXO3 could remarkably inhibit PCa cell cycle, proliferation and promote cell apoptosis in vitro. Furthermore, we demonstrated circFOXO3 could act as miR-29a-3p sponge to up-regulate SLC25A15 expression by bioinformatics analysis, dual-luciferase reporter assays and biotinylated RNA pull-down assays. SLC25A15 could reverse the tumour suppressing roles of knock-down circFOXO3 in PCa. Of note, we found that miR-29a-3p was down-regulated; however, SLC25A15 was overexpressed in PCa samples compared with normal tissues. In conclusion, circFOXO3 acts as a miR-29a-3p sponge to exhibit oncogenic activity that affects the cell cycle and cell apoptosis in PCa through transcriptional up-regulation of SLC25A15. Our analysis suggests circFOXO3 could act as promising prostate cancer biomarkers.

18.
J Chromatogr A ; : 460618, 2019 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-31672267

RESUMO

In this study, a monolithic enzyme reactor based on a strategy of green synthesis was successfully prepared in a capillary with trypsin immobilized by "thiol-ene" click reaction. A polymer of poly(butyl methacrylate-co-α-methacrylic acid-co-ethylene glycol dimethacrylate) was prepared in a mixture of 1-butyl-3-methylimidazolium tetrafluoroborate and choline chloride/ethylene glycol as the support of enzyme reactor. After "thiol-ene" reaction was used for enzyme immobilization, the Michaelis constants and maximum reaction rate of the resulting immobilized enzyme reactors (IMER) were determined by capillary electrophoresis to be 2.1 mmol/L and 0.028 µmol/min, respectively. The enzymatic hydrolysis of the enzyme reactor under different experimental conditions were investigated. A on-line digestion of bovine serum albumin (BSA) on the new IMER can be achieved within 50 s, up to 864 times faster than in-solution digestion (12 h). BSA can be well digested and the numbers of identified peptides were 73 with the coverage rates of 82.7%. The IMER was further used for the analysis of protein extracts from rat liver, and 1034 protein groups were identified. All these results demonstrated that such a click reaction based IMER would be of great prospect in the high throughput analysis for proteome with high confidence.

20.
Opt Express ; 27(19): 26676-26688, 2019 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-31674543

RESUMO

We undertake a computational and experimental study of an advanced class of structured beams, partially coherent radially polarized vortex (PCRPV) beams, on propagation through atmospheric turbulence. A computational propagation model is established to simulate this class of beams, and it is used to calculate the average intensity and on-axis scintillation index of PCRPV beams. On comparison with other classes of structured beams, such as partially coherent vortex beams and partially coherent radially polarized beams, it is found that the PCRPV beams, which structure phase, coherence and polarization simultaneously, show marked improvements in atmospheric propagation. The simulation results agree reasonably well with the experimental results. These beams will be useful in free-space optical communications and remote sensing.

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