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1.
Org Biomol Chem ; 20(3): 565-569, 2022 Jan 19.
Artigo em Inglês | MEDLINE | ID: mdl-34985096

RESUMO

A thioether directed acyloxylation of arenes has been realized via Cp*Rh(III)-catalyzed C-H activation and subsequent coupling with carboxylic acids. This new method showed high functional group compatibility and broad substrate scope. Primary mechanistic studies have been conducted and a tentative reaction mechanism was proposed. It represents the first example of a thioether-directed Cp*Rh(III)-catalyzed C(sp2)-H acyloxylation reaction.

2.
Org Biomol Chem ; 19(39): 8487-8491, 2021 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-34545904

RESUMO

An efficient and practical approach for the synthesis of medicinally important acridones was developed from anthranils and commercially available arylboronic acids by a tandem copper(I)-catalyzed electrophilic amination/Ag(I)-mediated oxidative annulation strategy. This new and straightforward protocol displayed a broad substrate scope (25 examples) and high functional group tolerance. What's more, a possible mechanistic proposal was also presented.

3.
Front Genet ; 12: 688241, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34306029

RESUMO

Background: The low-density lipoprotein receptor-related protein 6 (LRP6) gene is a recently defined gene that is associated with the autosomal-dominant inherited tooth agenesis (TA). In the present study, a family of four generations having TA was recruited and subjected to a series of clinical, genetic, in silico, and in vitro investigations. Methods: After routine clinical evaluation, the proband was subjected to whole-exome sequencing (WES) to detect the diagnostic variant. Next, in silico structural and molecular dynamics (MD) analysis was conducted on the identified novel missense variant for predicting its intramolecular impact. Subsequently, an in vitro study was performed to further explore the effect of this variant on protein maturation and phosphorylation. Results: WES identified a novel variant, designated as LRP6: c.2570G > A (p.R857H), harbored by six members of the concerned family, four of whom exhibited varied TA symptoms. The in silico analysis suggested that this novel variant could probably damage the Wnt bonding function of the LRP6 protein. The experimental study demonstrated that although this novel variant did not affect the LRP6 gene transcription, it caused a impairment in the maturation and phosphorylation of LRP6 protein, suggesting the possibility of the disruption of the Wnt signaling. Conclusion: The present study expanded the mutation spectrum of human TA in the LRP6 gene. The findings of the present study are insightful and conducive to understanding the functional significance of specific LRP6 variants.

4.
Aging Cell ; 20(8): e13430, 2021 08.
Artigo em Inglês | MEDLINE | ID: mdl-34278704

RESUMO

Dental pulp stem cells (DPSCs) play a vital role in tooth restoration, regeneration, and homeostasis. The link between DPSC senescence and tooth aging has been well-recognized. ROR2 plays an important role in aging-related gene expression. However, the expression and function of ROR2 in DPSC aging remain largely unknown. In this study, we found that ROR2 expression was significantly decreased in aged pulp tissues and DPSCs. The depletion of ROR2 in young DPSCs inhibits their self-renewal capacity, while its overexpression in aged DPSCs restores their self-renewal capacity. Interestingly, we found that sphingomyelin (SM) is involved in the senescence of DPSCs regulated by ROR2. Mechanistically, we confirmed that ROR2 inhibited the phosphorylation of STK4, which promoted the translocation of Forkhead Box O1 (FOXO1) to the nucleus. STK4 inhibition or knockdown of FOXO1 markedly increased the proliferation of DPSCs and upregulated the expression of SMS1, which catalyzed SM biogenesis. Moreover, FOXO1 directly bound to the SMS1 promoter, repressing its transcription. Our findings demonstrated the critical role of the ROR2/STK4-FOXO1/SMS1 axis in the regulation of SM biogenesis and DPSC senescence, providing a novel target for antagonizing tooth aging.

5.
World J Clin Cases ; 9(17): 4188-4198, 2021 Jun 16.
Artigo em Inglês | MEDLINE | ID: mdl-34141781

RESUMO

BACKGROUND: Cervical cancer is the fourth commonest malignancy in women around the world. It represents the second most commonly diagnosed cancer in South East Asian women, and an important cancer death cause in women of developing nations. Data collected in 2018 revealed 5690000 cervical cancer cases worldwide, 85% of which occurred in developing countries. AIM: To assess self-perceived burden (SPB) and related influencing factors in cervical cancer patients undergoing radiotherapy. METHODS: Patients were prospectively included by convenient sampling at The Fifth Affiliated Hospital of Sun Yat-Sen University, China between March 2018 and March 2019. The survey was completed using a self-designed general information questionnaire, the SPB scale for cancer patients, and the self-care self-efficacy scale, Strategies Used by People to Promote Health, which were delivered to patients with cervical cancer undergoing radiotherapy. Measurement data are expressed as the mean ± SD. Enumeration data are expressed as frequencies or percentages. Caregivers were the spouse, offspring, and other in 46.4, 40.9, and 12.7%, respectively, and the majority were male (59.1%). As for pathological type, 90 and 20 cases had squamous and adenocarcinoma/adenosquamous carcinomas, respectively. Stage IV disease was found in 12 (10.9%) patients. RESULTS: A total of 115 questionnaires were released, and five patients were excluded for too long evaluation time (n = 2) and the inability to confirm the questionnaire contents (n = 3). Finally, a total of 110 questionnaires were collected. They were aged 31-79 years, with the 40-59 age group being most represented (65.4% of all cases). Most patients were married (91.8%) and an overwhelming number had no religion (92.7%). Total SPB score was 43.13 ± 16.65. SPB was associated with the place of residence, monthly family income, payment method, transfer status, the presence of radiotherapy complications, and the presence of pain (P < 0.05). The SPB and self-care self-efficacy were negatively correlated (P < 0.01). In multivariate analysis, self-care self-efficacy, place of residence, monthly family income, payment method, degree of radiation dermatitis, and radiation proctitis were influencing factors of SPB (P < 0.05). CONCLUSION: Patients with cervical cancer undergoing radiotherapy often have SPB. Self-care self-efficacy scale, place of residence, monthly family income, payment method, and radiation dermatitis and proctitis are factors independently influencing SPB.

6.
Front Cell Dev Biol ; 9: 809656, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34977045

RESUMO

Stem cell therapies have shown promising therapeutic effects in restoring damaged tissue and promoting functional repair in a wide range of human diseases. Generations of insulin-producing cells and pancreatic progenitors from stem cells are potential therapeutic methods for treating diabetes and diabetes-related diseases. However, accumulated evidence has demonstrated that multiple types of programmed cell death (PCD) existed in stem cells post-transplantation and compromise their therapeutic efficiency, including apoptosis, autophagy, necroptosis, pyroptosis, and ferroptosis. Understanding the molecular mechanisms in PCD during stem cell transplantation and targeting cell death signaling pathways are vital to successful stem cell therapies. In this review, we highlight the research advances in PCD mechanisms that guide the development of multiple strategies to prevent the loss of stem cells and discuss promising implications for improving stem cell therapy in diabetes and diabetes-related diseases.

7.
Int J Oncol ; 53(6): 2615-2626, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30320366

RESUMO

Retinoblastoma (RB) is a well­vascularized tumor dependent on angiogenesis. The present study aimed to explore whether microRNA (miR)­182 regulates cell viability, invasion and angiogenesis in RB via the phosphatidylinositol­3­OH kinase (PI3K)/protein kinase B (AKT) signaling pathway and by targeting cell adhesion molecule 2 (CADM2). The expression levels of miR­182 and CADM2 were initially detected in RB tissues from patients with RB who underwent ophthalmectomy, and normal retinal tissues collected from other trauma patients who underwent eye enucleation. To determine whether CADM2 was targeted by miR­182, a dual luciferase reporter assay was conducted. Subsequently, Y79 and WERI­Rb­1 RB cells were transfected with a miR­182 mimic or miR­182 inhibitor, or small interfering RNA against CADM2, in order to investigate the effects of miR­182 on viability and invasion, which were detected using MTT and Transwell assays, respectively. In addition, to determine whether the regulatory mechanism underlying the effects of miR­182 was associated with the PI3K/AKT signaling pathway, the expression levels of associated genes were detected by reverse transcription­quantitative polymerase chain reaction and western blot analysis. A xenograft tumor model in nude mice was also established, in order to evaluate the effects of miR­182 on tumor growth and angiogenesis. The results indicated that miR­182 expression was increased and CADM2 expression was reduced in RB tissues; CADM2 was confirmed to be targeted and negatively regulated by miR­182. When the expression of miR­182 was downregulated, cell viability, invasion, tumor volume and angiogenesis were significantly decreased. Furthermore, the expression levels of PI3K/AKT signaling pathway­associated genes were increased in response to miR­182 overexpression or CADM2 silencing. Taken together, these results suggested that inhibition of miR­182 may suppress cell viability, invasion and angiogenesis in RB through inactivation of the PI3K/AKT pathway and CADM2 upregulation. This mechanism may reveal a novel potential therapeutic target.


Assuntos
Moléculas de Adesão Celular/genética , Moléculas de Adesão Celular/metabolismo , MicroRNAs/genética , Retinoblastoma/patologia , Transdução de Sinais , Regiões 3' não Traduzidas , Animais , Linhagem Celular Tumoral , Sobrevivência Celular , Pré-Escolar , Regulação para Baixo , Enucleação Ocular , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Masculino , Camundongos , Camundongos Nus , Invasividade Neoplásica , Transplante de Neoplasias , Fosfatidilinositol 3-Quinases/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Retinoblastoma/genética , Retinoblastoma/metabolismo , Retinoblastoma/cirurgia , Regulação para Cima
8.
Am J Physiol Cell Physiol ; 315(6): C839-C849, 2018 12 01.
Artigo em Inglês | MEDLINE | ID: mdl-30183321

RESUMO

Glaucoma represents a major cause of blindness, generally associated with elevated intraocular pressure (EIOP). The aim of the present study was to investigate whether microRNA-149 (miR-149) affects retinal ganglion cells (RGCs) and the underlying mechanism based on a mouse model of chronic glaucoma with EIOP. The successfully modeled mice were administered with mimics or inhibitors of miR-149. Next, the number of RGCs, ultrastructural changes of RGCs, and purity of RGCs in the retinal tissues were detected. Moreover, the RGCs were collected and subsequently treated with 60 mmHg pressure and transfected with a series of plasmids aiding in the regulation of the expression of miR-149 and betacellulin (BTC). The levels of miR-149, BTC, phosphatidylinositol 3-kinase (PI3K), and Akt were subsequently determined. Finally, RGC viability and apoptosis were detected accordingly. Dual luciferase reporter gene assay provided validation, highlighting BTC was indeed a target gene of miR-149. The downregulation of miR-149 is accompanied by an increased number of RGCs and decreased ultrastructural RGC alterations. Additionally, downregulated miR-149 was noted to increase the levels of BTC, PI3K, and Akt in both the retinal tissues and RGCs, whereas the silencing of miR-149 was observed to promote the viability of RGC and inhibit RGC apoptosis. Taken together, the results of the current study provided validation suggesting that the downregulation of miR-149 confers protection to RGCs by means of activating the PI3K/Akt signaling pathway via upregulation of BTC in mice with glaucoma. Evidence presented indicated the promise of miR-149 inhibition as a potential therapeutic strategy for glaucoma treatment.


Assuntos
Apoptose/genética , Betacelulina/genética , Glaucoma/genética , MicroRNAs/genética , Animais , Sobrevivência Celular/genética , Modelos Animais de Doenças , Regulação da Expressão Gênica , Glaucoma/patologia , Humanos , Hipertensão Intracraniana/genética , Hipertensão Intracraniana/patologia , Camundongos , Proteína Oncogênica v-akt/genética , Fosfatidilinositol 3-Quinases/genética , Células Ganglionares da Retina/metabolismo , Células Ganglionares da Retina/patologia , Transdução de Sinais/genética
9.
Int J Immunopathol Pharmacol ; 32: 2058738418790318, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30045644

RESUMO

Targeting of the programmed cell-death 1 ligand 1 (PD-L1) signal pathway is a promising treatment strategy in several cancers. The purpose of this study was to evaluate the clinical significance of PD-L1 in patients with colon adenocarcinoma (COAD). A total of 240 patients who were diagnosed with COAD from The Cancer Genome Atlas (TCGA) RNA-sequencing data and another cohort for pair-matched COAD samples (n = 40) in tissue microarray (TMA) were enrolled in this study. The correlation of PD-L1 or miR-191-5p expression with clinicopathological features and prognosis in patients with COAD was further analyzed using TCGA data and TMA. The Cox proportional hazard regression model was used to evaluate the association of PD-L1 or miR-191-5p expression with overall survival (OS) and tumor recurrence in patients with COAD. The microRNAs (miRNAs) that target PD-L1 gene were identified by bioinformatics and Spearman correlation analysis. We found that PD-L1 expression was increased in COAD tissues and was correlated with poor survival and tumor recurrence in patients with COAD. The increased expression of PD-L1 was attributed to the dysregulation of miR-191-5p expression rather than its genetic or epigenetic alterations. Moreover, the expression of miR-191-5p presented the negative correlation with PD-L1 expression and acted as an independent prognostic factor of OS in patients with COAD. Therefore, PD-L1 may predict poor prognosis and is negatively associated with miR-191-5p expression in patients with COAD.


Assuntos
Adenocarcinoma/genética , Antígeno B7-H1/genética , Neoplasias Colorretais/genética , MicroRNAs/genética , Recidiva Local de Neoplasia/genética , Linhagem Celular Tumoral , Humanos , Estimativa de Kaplan-Meier , Prognóstico , Modelos de Riscos Proporcionais , Regulação para Cima
10.
Int J Oncol ; 51(5): 1563-1573, 2017 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-29048657

RESUMO

Aberrant activation of ß-catenin signaling due to low expression of miR­200a is found in gastric carcinoma (GC) tissues promoting GC evolution. Toosendanin (TSN) has exhibited antitumor effects on various human cancer cells, but its influence on GC is largely unidentified. The potential roles of TSN on GC cells were examined and it was found that TSN inhibited growth, migration, invasion and TGF­ß1-induced epithelial-mesenchymal transition (EMT) and induced cell cycle arrest and apoptosis in SGC­7901 cells which were most sensitive to TSN among various GC cell lines. TSN also inactivated ß-catenin pathway in SGC­7901 cells and the above effects were reversed following induction of ß-catenin overexpression. Moreover, TSN facilitated the level of miR­200a which targets ß-catenin and miR­200a silencing attenuated the antitumor effects of TSN on SGC­7901 cells. Nonetheless, knockdown of miR­200a did not relieve the suppressive effects of TSN on p­AKT, p­ERK and p­GSK3ß which were upstream regulators of ß-catenin. In addition, TSN administration inhibited growth and liver metastasis of orthotopically implanted SGC­7901 tumors in vivo through miR­200a­mediated ß-catenin pathway. Our data suggest that TSN may suppress oncogenic phenotypes of human GC cells partly via miR­200a/ß-catenin axis. Hence, TSN may have a promising chemotherapeutic activity for GC therapy.


Assuntos
Carcinoma/tratamento farmacológico , Medicamentos de Ervas Chinesas/administração & dosagem , MicroRNAs/genética , Neoplasias Gástricas/tratamento farmacológico , beta Catenina/genética , Animais , Apoptose/efeitos dos fármacos , Carcinoma/genética , Carcinoma/patologia , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Transição Epitelial-Mesenquimal/efeitos dos fármacos , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Humanos , Camundongos , Invasividade Neoplásica/genética , Metástase Neoplásica , Neoplasias Gástricas/genética , Neoplasias Gástricas/patologia , Via de Sinalização Wnt/efeitos dos fármacos , Ensaios Antitumorais Modelo de Xenoenxerto
12.
J Cell Mol Med ; 21(6): 1117-1127, 2017 06.
Artigo em Inglês | MEDLINE | ID: mdl-27957827

RESUMO

Sanguinarine, a bioactive benzophenanthridine alkaloid extracted from plants of the Papaveraceae family, has shown antitumour effects in multiple cancer cells. But the therapeutic effects and regulatory mechanisms of sanguinatine in gastric cancer (GC) remain elusive. This study was aimed to investigate the correlation of dual-specificity phosphatase 4 (DUSP4) expression with clinicopathologic features and overall survival in patients with GC and explore the effects of sanguinarine on tumour growth and invasion in GC cells (SGC-7901 and HGC-27) and underlying molecular mechanisms. Immunohistochemical analysis showed that decreased DUSP4 expression was associated with the sex, tumour size, depth of invasion and distant metastasis in patients with GC. Functional experiments including CCK-8, Transwell and flow cytometry analysis indicated that sanguinarine or DUSP4 overexpression inhibited GC cell viability and invasive potential, and induced cell apoptosis and cycle arrest in S phase, but DUSP4 knockdown attenuated the antitumour activity of sanguinarine. Further observation demonstrated that sanguinarine up-regulated the expression of DUSP4 and Bcl-2-associated X protein (Bax), but down-regulated phosphorylated extracellular signal-regulated kinase (p-ERK), proliferating cell nuclear antigen (PCNA), matrix metalloproteinase 2 (MMP-2) and B-cell lymphoma 2 (Bcl-2) expression. Taken together, our findings indicate that sanguinarine inhibits growth and invasion of GC cells through regulation of the DUSP4/ERK pathway, suggesting that sanguinarine may have potential for use in GC treatment.


Assuntos
Benzofenantridinas/administração & dosagem , Proliferação de Células/efeitos dos fármacos , Fosfatases de Especificidade Dupla/genética , Isoquinolinas/administração & dosagem , Fosfatases da Proteína Quinase Ativada por Mitógeno/genética , Neoplasias Gástricas/tratamento farmacológico , Idoso , Linhagem Celular Tumoral , Proliferação de Células/genética , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Masculino , Pessoa de Meia-Idade , Invasividade Neoplásica/genética , Proteínas de Neoplasias/genética , Proteínas Proto-Oncogênicas c-bcl-2/genética , Neoplasias Gástricas/genética , Neoplasias Gástricas/patologia
13.
Mol Immunol ; 78: 133-139, 2016 10.
Artigo em Inglês | MEDLINE | ID: mdl-27639060

RESUMO

MicroRNAs (miRNAs) are small noncoding RNAs that function in diverse biological processes. However, little is known about the precise role of microRNAs in the functioning of airway smooth muscle cells (ASMCs). Here, we investigated the potential role and mechanisms of the miR-143 -3p on proliferation and the extracellular matrix (ECM) protein production of ASMCs. We demonstrated that miR-143-3p was aberrantly lower in ASMCs isolated from individuals with asthma than in individuals without asthma. Meanwhile, TGF-ß1 caused a marked decrease in a time-dependent manner in miR-143-3p expression in ASMCs from asthmatics. Additionally, the overexpression of miR- 143-3p robustly reduced TGF-ß1-induced ASMCs proliferation and downregulated CDK and cyclin expression, whereas the inhibition of miR-143-3p significantly enhanced ASMCs proliferation and upregulated the level of CDKs and cyclins. Re-expression of miR-143-3p attenuated ECM protein deposition reflected as a marked decrease in the expression of type I collagen and fibronectin, whereas miR-143-3p downregulation caused an opposite effect on the expression of type I collagen and fibronectin. Moreover, qRT-PCR and western blot analysis indicated that miR-143-3p negatively regulated the expression of nuclear factor of activated T cells 1 (NFATc1). Subsequent analyses demonstrated that NFATc1 was a direct and functional target of miR-143-3p, which was validated by the dual luciferase reporter assay. Most importantly, the overexpression of NFATc1 effectively reversed the inhibition of miR-143-3p on TGF-ß1-induced proliferation, and strikingly abrogated the effect of miR-143-3p on the expression of CDK4 and Cyclin D1. Together, miR-143-3p may function as an inhibitor of asthma airway remodeling by suppressing proliferation and ECM protein deposition in TGF-ß1-mediated ASMCs via the negative regulation of NFATc1 signaling, suggesting miR-143-3p as a potential therapeutic target for asthma.


Assuntos
Remodelação das Vias Aéreas/imunologia , Asma/imunologia , Regulação da Expressão Gênica/imunologia , MicroRNAs/imunologia , Fatores de Transcrição NFATC/biossíntese , Adulto , Asma/genética , Asma/patologia , Western Blotting , Brônquios/imunologia , Brônquios/metabolismo , Brônquios/patologia , Proliferação de Células/fisiologia , Células Cultivadas , Regulação para Baixo , Ensaio de Imunoadsorção Enzimática , Matriz Extracelular/patologia , Feminino , Humanos , Masculino , MicroRNAs/metabolismo , Músculo Liso/imunologia , Músculo Liso/patologia , Fatores de Transcrição NFATC/imunologia , Reação em Cadeia da Polimerase em Tempo Real , Fator de Crescimento Transformador beta1/imunologia , Fator de Crescimento Transformador beta1/metabolismo
14.
J Exp Clin Cancer Res ; 35: 47, 2016 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-26980572

RESUMO

BACKGROUND: MicroRNAs (miRNAs) have been proved involved in many tumorigenic behaviors including tumor growth. But, the clinical significance and functions of miRNA-203 in gastric cancer (GC) remain elusive. RESULTS: Decreased expression of miRNA-203 was correlated with tumor size, poor prognosis and recurrence in GC patients. Overexpression of miR-203 or knockdown of its target progesterone immunomodulatory binding factor 1 (PIBF1) inhibited GC growth in vitro and in vivo, while miR-203 knockdown promoted GC proliferation. In addition, PIBF1 overexpression attenuated the inhibitory effects of miR-203 on GC growth and enhanced that effect on p-Akt expression. CONCLUSIONS: MiR-203 as a tumor biomarker suppresses GC growth through targeting the PIBF1/Akt signaling, suggesting that it may have the important therapeutic potential for the treatment of GC.


Assuntos
MicroRNAs/genética , Recidiva Local de Neoplasia/genética , Proteínas da Gravidez/genética , Proteínas Proto-Oncogênicas c-akt/genética , Neoplasias Gástricas/patologia , Fatores Supressores Imunológicos/genética , Linhagem Celular Tumoral , Proliferação de Células , Feminino , Humanos , Masculino , Prognóstico , Transdução de Sinais , Neoplasias Gástricas/genética
15.
Neuroreport ; 26(17): 1032-8, 2015 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-26457369

RESUMO

Neuroinflammation mediated by activated microglia plays a pivotal role in the pathogenesis of neurological disorders, including hypoxic injury of the developing brain. Thymosin ß4 (Tß4), the major G-actin-sequestering molecule, has an anti-inflammatory effect and has been used to treat various neurological diseases. However, the effect of Tß4 on hypoxia-induced microglia activation in the developing brain remains unclear. We investigate here the effect of Tß4 on microglia activation of neonatal rats after hypoxia exposure. Tß4 treatment was carried out on 1-day-old rats and BV-2 cells. Tß4 expression in microglia was determined by quantitative real time-PCR, western blotting, and immunofluorescence staining. Secretion of tumor necrosis factor-α (TNF-α), interleukin-1ß (IL-1ß), and nitric oxide (NO) was assessed by enzyme-linked immunosorbent assay and colorimetric assay. mRNA expression of TNF-α and IL-1ß, and microRNA 146a expression was determined by quantitative real time-PCR. We showed that Tß4 treatment significantly inhibited secretion of inflammatory mediators in the cerebellum of neonatal rats following hypoxia injury. Increased expression of endogenous Tß4 in microglia was observed both in hypoxic rats and in BV-2 cells. Tß4 treatment significantly inhibited the expression and secretion of hypoxia-induced TNF-α, IL-1ß, and NO. Remarkably, microRNA 146a expression was found to have increased in Tß4-treated BV-2 cells. We demonstrated the anti-inflammatory effect of Tß4 in neonatal rats following hypoxic brain injury. More importantly, our data reveal, for the first time, that Tß4 inhibits microglia activation in vitro. Therefore, this study contributes to understanding the role and mechanism of Tß4 function in central nervous system diseases.


Assuntos
Cerebelo/metabolismo , Encefalite/metabolismo , Hipóxia Encefálica/metabolismo , MicroRNAs/metabolismo , Microglia/metabolismo , Timosina/metabolismo , Animais , Animais Recém-Nascidos , Hipóxia Celular , Células Cultivadas , Encefalite/etiologia , Hipóxia Encefálica/complicações , Mediadores da Inflamação/metabolismo , Interleucina-1beta/metabolismo , Óxido Nítrico/metabolismo , RNA Mensageiro/metabolismo , Ratos , Fator de Necrose Tumoral alfa/metabolismo
16.
Ying Yong Sheng Tai Xue Bao ; 25(5): 1468-74, 2014 May.
Artigo em Chinês | MEDLINE | ID: mdl-25129950

RESUMO

Bacterial consortium EG03, consisted of several different antagonistic bacteria against Ralstonia solanacearum, was demonstrated to efficiently control bacterial wilt of pepper in field with a biocontrol efficacy of 85.8%. The traditional dilution plate method, the most probable number (MPN) method and Biolog system were adopted to determine effects of EG03 on characteristics of microbial community in pepper rhizosphere. It's shown that EGO3's effects on microbial community in pepper rhizospheric soil varied with time. There were an increase in the number of fungus and Bacillus spp. to some extent and a significant increase in that of nitrogen-fixing bacteria. Biolog analysis showed that the curve between average well color development (AWCD) and incubation time was S-shaped for all the treatments and that the AWCD of pepper rhizospheric soil at the early stage was higher than at the late stage. The analysis of carbon source utilization showed that EG03 decreased microbial utilization of carbon source in short-term, and the microbial community of pepper rhizospheric soil at the late stage composed mainly of microbes depended on sugars as carbon resource. EG03 treatment could decrease the five microbial diversity indices of rhizospheric microbes in short term, then increased those indices instead, especially with significant (P < 0.05) increases in Simpson index and McIntosh evenness.


Assuntos
Agentes de Controle Biológico , Consórcios Microbianos , Doenças das Plantas/microbiologia , Rizosfera , Microbiologia do Solo , Bactérias , Capsicum/microbiologia , Carbono , Fungos , Nitrogênio , Ralstonia solanacearum/patogenicidade , Solo
17.
Zhonghua Yi Xue Za Zhi ; 93(9): 693-7, 2013 Mar 05.
Artigo em Chinês | MEDLINE | ID: mdl-23751750

RESUMO

OBJECTIVE: To explore the effects of blood, crystalloid and colloid on mesenteric microcirculation in rabbits of acute hemorrhagic shock. METHODS: Hemorrhagic shock was induced in 28 anesthetized mechanically ventilated rabbits by withdrawing blood to a mean arterial blood pressure (MAP) of 40 mm Hg and maintained for 30 min. The animals were randomized into blank control group, whole blood resuscitation group, Ringer's solution resuscitation (RR) group and volume resuscitation (VR) group (n = 7 each). The data of vital signs, systemic hemodynamic parameters, arterial blood gas analysis and mesenteric microcirculation were collected at baseline, hemorrhagic shock (HS-0), resuscitation 0 min (Res-0) and resuscitation 30 min (Res-30). And side-stream dark field imaging (SDF) was employed to acquire the images of mesenteric microcirculation. RESULTS: No significant difference existed in the basic status between 4 groups. MAP decreased to around 40 mm Hg in all groups. MAP increased significantly after active fluid resuscitation (P < 0.05). In addition to blank control group, total vascular density (TVD), perfused vessel density (PVD), proportion of perfused vessels (PPV) and microvascular flow index (MFI) significantly increased after active fluid resuscitation in the other three groups. TVD and PVD were significantly lower at Res-0 in VR than in RR (P < 0.05). CONCLUSION: After acute hemorrhagic shock, fluid resuscitation with whole blood and Ringer's solution improves systemic hemodynamics. And the combined regimen of whole blood and Ringer's solution is better at restoring mesenteric microcirculation.


Assuntos
Hidratação/métodos , Mesentério/irrigação sanguínea , Microcirculação , Choque Hemorrágico/terapia , Animais , Modelos Animais de Doenças , Soluções Isotônicas , Coelhos , Ressuscitação/métodos , Solução de Ringer
18.
Zhonghua Yi Xue Za Zhi ; 93(8): 600-2, 2013 Feb 26.
Artigo em Chinês | MEDLINE | ID: mdl-23663341

RESUMO

OBJECTIVE: To explore the efficacies and safety of combined treatment of remifemin and paroxetine for perimenopausal depression. METHODS: A total of 120 patients with perimenopausal depression were digital randomly divided into the treatment and control groups (n = 60 each). The treatment group received oral remifemin one tablet twice daily and paroxetine 20 mg once daily for 2 months while the control group oral paroxetine 20 mg once daily for 2 months. The Hamilton depression scale (HAMD) and Kupperman scale were used to assess the therapeutic efficacies. Blood and urine routine, electrocardiography, liver function, kidney function and blood pressure before and after treatment were examined to assess the side effects. RESULTS: For the improvement of perimenopausal depression on HAMD, the total effective rates of the treatment and control groups were 88.3% and 78.3% respectively. The therapeutic efficacy of the treatment group was significantly higher than that of the control group (P < 0.05). After 8-week treatment, Kupperman menopausal indices of the treatment and control groups were 9.89 ± 3.76 and 15.75 ± 5.84 respectively. There was also significant difference (P < 0.01). No significant changes existed in blood routine, urine routine, liver function, kidney function, blood pressure, ECG or blood pressure before and after treatment (P > 0.05). CONCLUSION: The combined treatment of remifemin and paroxetine for perimenopausal depression can improve the efficacies. It is easily accepted by patients for its higher safety and fewer side-effects. It is worthy of a wider application and further researches.


Assuntos
Transtorno Depressivo Maior/tratamento farmacológico , Paroxetina/uso terapêutico , Extratos Vegetais/uso terapêutico , Cimicifuga , Feminino , Humanos , Pessoa de Meia-Idade , Perimenopausa , Resultado do Tratamento
20.
Zhonghua Zheng Xing Wai Ke Za Zhi ; 26(1): 1-4, 2010 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-20432914

RESUMO

OBJECTIVE: To investigate the feasibility and significance of modified paramedian forehead flap for nasal defect and reconstruction. METHODS: Modified paramedian forehead flaps with forehead muscle in the pedicle were used in 2 patients with nasal reconstruction and 7 patients with nasal defects. The flaps were elevated subcutaneously with only forehead muscle in the pedicle at the supraorbital site. The degree of flap axis ranged from 90 degrees to 50 degrees. Inverted L-shape design was used for 3 cases with low hair line. RESULTS: Skin branch of supratrochlear vessel was observed running at the subcutaneous layer during the operation. 8 flaps all survived successfully with good texture and color. Partial necrosis happened in the distal end of one flap with subcutaneous pedicle, which healed through dressing. CONCLUSIONS: Modified paramedian forehead flap, which includes muscle just in the pedicle, is based on the skin branch of supratrochlear vessel. The flap is very flexible and has reliable blood supply, leaving less morbidity in donor site. It also has good texture and skin color.


Assuntos
Testa/cirurgia , Nariz/cirurgia , Rinoplastia/métodos , Retalhos Cirúrgicos , Adolescente , Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Transplante de Pele/métodos , Adulto Jovem
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