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1.
J Craniofac Surg ; 30(7): 2253-2256, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31513037

RESUMO

This paper describes the application of a modified digital surgical guide, an occlusal template and a pre-formed reconstruction titanium plate in the open reduction and internal fixation of a bilateral mandibular fracture. Bilateral mandibular fracture is a commonly encountered simple type of fracture. However, even for an experience surgeon, achieving precise treatment remains a challenge due to the movable temporomandibular joint, unstable fractured segments, the difficulty in forming a reconstruction plate, and the lack of an effective stabilizing and locating device. In this case, the surgeon used a specially-designed modified guide together with a reconstruction plate and an occlusal template to treat a bilateral mandible fracture, effectively improving the accuracy and the medical outcome of the operation, saving operation time and reducing postoperative complications.


Assuntos
Fraturas Mandibulares/cirurgia , Reconstrução Mandibular , Adulto , Placas Ósseas , Fixação Interna de Fraturas , Humanos , Masculino , Fraturas Mandibulares/diagnóstico , Reconstrução Mandibular/instrumentação , Duração da Cirurgia , Complicações Pós-Operatórias , Cirurgia Assistida por Computador , Articulação Temporomandibular/cirurgia , Titânio
2.
BMC Endocr Disord ; 19(1): 83, 2019 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-31362731

RESUMO

BACKGROUND: Thyroid dysfunction is one of the prevalent endocrine disorders. The relationship between lifestyle factors and thyroid dysfunction was not clear and some of the factors seemed paradoxical. METHODS: We conducted this population-based study using data from 5154 She ethnic minority people who had entered into the epidemic survey of diabetes between July 2007 to September 2009. Life style information was collected using a standard questionnaire. Body mass index (BMI), Blood pressure and serum TSH, TPOAb, triglycerides (TG), total cholesterol (TC) and high-density lipoprotein cholesterol (HDL) were collected. RESULTS: The study showed that people who drank, had higher education or suffered from insomnia have lower incidence of hyperthyroidism. On the other hand, smoking, alcohol consumption, exercise, undergoing weight watch and chronic headache were associated with decreased incidence of hypothyroidism. Using multivariable logistic regression analysis, we found that alcohol consumption was associated with decreased probability of hyperthyroidism, hypothyroidism, as well as positive TPOAb. The amounts of cigarettes smoked daily displayed a positive correlation with hyperthyroidism among smokers. Accordingly, smoking seemed to be associated with decreased risk for hypothyroidism and positive TPOAb. Exercise and maintaining a healthy weight might have a beneficial effect on thyroid health. Interestingly, daily staple amount showed an inverse correlation with incidence of positive TPOAb. CONCLUSIONS: Within the Chinese She ethnic minority, we found associations between different lifestyle factors and the incidence of different thyroid diseases. Understanding the nature of these associations requires further investigations.

3.
Int J Mol Med ; 44(3): 857-870, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31257525

RESUMO

Although miRNAs have been implicated in the osteogenic differentiation of stem cells, their role in bone repair and reconstruction in tissue­engineered bone grafts remains unclear. We previously reported that microRNA (miR)­26a­5p inhibited the osteogenic differentiation of adipose­derived mesenchymal stem cells (ADSCs), and that antimiR­26a­5p exerted the opposite effect. In the present study, the role of miR­26a­5p­ and antimiR­26a­5p­modified ADSCs combined with biphasic calcium phosphate (BCP) scaffolds was evaluated in a rat femur defect model. The aim of the present study was to improve the understanding of the role of miR­26a­5p in bone regeneration in vivo, as well as to provide a new method to optimize the osteogenic ability of BCPs. ADSCs were infected with Lv­miR­26a­5p, Lv­miR­NC, Lv­antimiR­26a­5p or Lv­antimiR­NC respectively, and then combined with BCP scaffolds to repair rat femoral defects. Using X­rays, micro­computed tomography and histology at 2, 4, and 8 weeks postoperatively, the quantity and rate of bone regeneration were analyzed, revealing that they were the highest in animals treated with antimiR­26a­5p and the lowest in the miR­26a­5p treatment group. The expression levels of osteocalcin, collagen I, Runt­related transcription factor 2, Wnt family member 5A and calmodulin­dependent protein kinase II proteins were positively correlated with the bone formation rate. Taken together, the present results demonstrated that miR­26a­5p inhibited bone formation while antimiR­26a­5p accelerated bone formation via the Wnt/Ca2+ signaling pathway. Therefore, antimiR­26a­5p­modified ADSCs combined with BCP scaffolds may be used to construct an effective tissue­engineering bone graft for bone repair and reconstruction.

4.
Menopause ; 2018 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-30516712

RESUMO

OBJECTIVE: To assess the effect of early life exposure to famine, as endured during 1959 to 1961 in China, on reproductive aging in adult women. METHODS: Between 2011 and 2012, 2,868 women born around the Chinese famine period (1956-1964) were enrolled in this study from three communities in China. Age at natural menopause was obtained retrospectively from a structured questionnaire. The associations of early life famine exposure with reproductive aging during adulthood were estimated, with adjustment of socioeconomic status, lifestyle factors, and body mass index. RESULTS: Women exposed to prenatal famine had a higher risk of early menopause (ie, natural menopause <45 years, odds ratio: 1.59, 95% confidence interval [CI]: 1.07, 2.36), and a nonsignificant trend of higher risk of premature ovarian failure (ie, natural menopause <40 y, odds ratio: 1.94, 95% CI: 0.93, 4.00), compared to unexposed women. Exposure to famine during childhood was not significantly associated with reproductive aging. In a secondary analysis focusing on the fetal exposure, prenatal famine exposure was associated with a higher risk of premature ovarian failure (odds ratio: 2.07, 95% CI: 1.08, 3.87), and a nonsignificant trend of higher risk of early menopause (odds ratio: 1.37, 95% CI: 0.98, 1.91), compared to those unexposed to prenatal famine. CONCLUSIONS: Our study showed that fetal exposure to famine was associated with an increased risk of early menopause. Such findings provided evidence in favor of the thrifty phenotype theory in reproductive aging and helped better understand the etiology of early menopause.

5.
Biol Trace Elem Res ; 176(2): 401-406, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-27660074

RESUMO

This study was conducted to investigate the effects of chromium from chromium propionate (CrPro) on serum lipids, carcass traits, and breast meat quality in heat-stressed birds. A total of 210 1-day-old male broilers were randomly allotted by initial body weight (BW) into 5 treatments with 7 replicates with 6 birds per replicate pen for 42 days. The treatments included a basal corn-soybean meal diet and basal diet supplemented with 0.2, 0.4, 0.8, or 1.6 mg Cr/kg diet. Birds had ad libitum access to feed and distilled-deionized water at normal conditions for 1-3 weeks with little or no stress, and then birds were housed under heat stress conditions with 35 ± 2 °C ambient temperature for 4-6 weeks. Results showed that serum triglyceride (TG) (P = 0.0006) and low-density lipoprotein cholesterol (LDLC) (P = 0.0038) concentrations were decreased linearly as Cr dose increased. Compared with other groups, birds receiving 0.8 or 1.6 mg Cr/kg had lower TG (P = 0.0015). Compared to control birds, birds fed diets with 0.2, 0.4, 0.8, or 1.6 mg Cr/kg supplementation had lower LDLC (P = 0.0006). However, the total cholesterol (TC) and high density lipoprotein cholesterol (HDLC) concentrations in serum were not affected by treatment (P > 0.05). No difference was observed in BW, the relative weights of breast muscle, thigh muscle and abdominal fat (P > 0.05), and breast meat quality (Ph15min, Ph24h, L*, a*, b*, cooking loss, shear force) among the treatments (P > 0.05). Results from this study indicated that CrPro supplementation could be beneficial to serum lipids metabolism of heat-stressed broiler chickens by decreasing TG and LDLC contents, but had no impacts on meat quality and carcass traits of the heat-stressed broilers.


Assuntos
LDL-Colesterol/sangue , Suplementos Nutricionais , Transtornos de Estresse por Calor/metabolismo , Carne/normas , Propionatos/administração & dosagem , Propionatos/farmacologia , Triglicerídeos/sangue , Animais , Galinhas , Transtornos de Estresse por Calor/sangue , Temperatura Alta , Masculino , Carne/análise , Propionatos/metabolismo
6.
Bioorg Med Chem ; 25(2): 496-513, 2017 01 15.
Artigo em Inglês | MEDLINE | ID: mdl-27914948

RESUMO

Triazolopyridine ethers with mGlu2 positive allosteric modulator (PAM) activity are disclosed. The synthesis, in vitro activity, and metabolic stability data for a series of analogs is provided. The effort resulted in the discovery of a potent, selective, and brain penetrant lead molecule BMT-133218 ((+)-7m). After oral administration at 10mg/kg, BMT-133218 demonstrated full reversal of PCP-stimulated locomotor activity and prevented MK-801-induced working memory deficits in separate mouse models. Also, reversal of impairments in executive function were observed in rat set-shifting studies at 3 and 10mg/kg (p.o.). Extensive plasma protein binding as the result of high lipophilicity likely limited activity at lower doses. Optimized triazolopyridine ethers offer utility as mGlu2 PAMs for the treatment of schizophrenia and merit further preclinical investigation.


Assuntos
Éteres/farmacologia , Piridinas/farmacologia , Receptores de Glutamato Metabotrópico/antagonistas & inibidores , Esquizofrenia/tratamento farmacológico , Triazóis/farmacologia , Administração Oral , Regulação Alostérica/efeitos dos fármacos , Animais , Modelos Animais de Doenças , Relação Dose-Resposta a Droga , Éteres/administração & dosagem , Éteres/química , Haplorrinos , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Modelos Moleculares , Estrutura Molecular , Piridinas/administração & dosagem , Piridinas/química , Ratos , Ratos Sprague-Dawley , Receptores de Glutamato Metabotrópico/metabolismo , Esquizofrenia/metabolismo , Relação Estrutura-Atividade , Triazóis/administração & dosagem , Triazóis/química
7.
Bioorg Med Chem Lett ; 26(17): 4165-9, 2016 09 01.
Artigo em Inglês | MEDLINE | ID: mdl-27496211

RESUMO

Herein we describe the structure activity relationships uncovered in the pursuit of an mGluR5 positive allosteric modulator (PAM) for the treatment of schizophrenia. It was discovered that certain modifications of an oxazolidinone-based chemotype afforded predictable changes in the pharmacological profile to give analogs with a wide range of functional activities. The discovery of potent silent allosteric modulators (SAMs) allowed interrogation of the mechanism-based liabilities associated with mGluR5 activation and drove our medicinal chemistry effort toward the discovery of low efficacy (fold shift) PAMs devoid of agonist activity. This work resulted in the identification of dipyridyl 22 (BMS-952048), a compound with a favorable free fraction, efficacy in a rodent-based cognition model, and low potential for convulsions in mouse.


Assuntos
Convulsivantes/química , Oxazolidinonas/química , Receptor de Glutamato Metabotrópico 5/metabolismo , Regulação Alostérica/efeitos dos fármacos , Animais , Convulsivantes/metabolismo , Convulsivantes/farmacologia , Humanos , Camundongos , Camundongos Endogâmicos C57BL , Oxazolidinonas/metabolismo , Oxazolidinonas/farmacologia , Ratos , Receptor de Glutamato Metabotrópico 5/agonistas , Receptor de Glutamato Metabotrópico 5/química , Recognição (Psicologia)/efeitos dos fármacos , Relação Estrutura-Atividade
8.
J Pharmacol Exp Ther ; 358(3): 371-86, 2016 09.
Artigo em Inglês | MEDLINE | ID: mdl-27411717

RESUMO

To identify novel targets for neuropathic pain, 3097 mouse knockout lines were tested in acute and persistent pain behavior assays. One of the lines from this screen, which contained a null allele of the adapter protein-2 associated kinase 1 (AAK1) gene, had a normal response in acute pain assays (hot plate, phase I formalin), but a markedly reduced response to persistent pain in phase II formalin. AAK1 knockout mice also failed to develop tactile allodynia following the Chung procedure of spinal nerve ligation (SNL). Based on these findings, potent, small-molecule inhibitors of AAK1 were identified. Studies in mice showed that one such inhibitor, LP-935509, caused a reduced pain response in phase II formalin and reversed fully established pain behavior following the SNL procedure. Further studies showed that the inhibitor also reduced evoked pain responses in the rat chronic constriction injury (CCI) model and the rat streptozotocin model of diabetic peripheral neuropathy. Using a nonbrain-penetrant AAK1 inhibitor and local administration of an AAK1 inhibitor, the relevant pool of AAK1 for antineuropathic action was found to be in the spinal cord. Consistent with these results, AAK1 inhibitors dose-dependently reduced the increased spontaneous neural activity in the spinal cord caused by CCI and blocked the development of windup induced by repeated electrical stimulation of the paw. The mechanism of AAK1 antinociception was further investigated with inhibitors of α2 adrenergic and opioid receptors. These studies showed that α2 adrenergic receptor inhibitors, but not opioid receptor inhibitors, not only prevented AAK1 inhibitor antineuropathic action in behavioral assays, but also blocked the AAK1 inhibitor-induced reduction in spinal neural activity in the rat CCI model. Hence, AAK1 inhibitors are a novel therapeutic approach to neuropathic pain with activity in animal models that is mechanistically linked (behaviorally and electrophysiologically) to α2 adrenergic signaling, a pathway known to be antinociceptive in humans.


Assuntos
Neuralgia/tratamento farmacológico , Inibidores de Proteínas Quinases/farmacologia , Proteínas Serina-Treonina Quinases/antagonistas & inibidores , Animais , Fenômenos Eletrofisiológicos/efeitos dos fármacos , Técnicas de Inativação de Genes , Células HEK293 , Humanos , Masculino , Camundongos , Neuralgia/metabolismo , Neuralgia/fisiopatologia , Nociceptividade/efeitos dos fármacos , Fenótipo , Inibidores de Proteínas Quinases/uso terapêutico , Proteínas Serina-Treonina Quinases/deficiência , Proteínas Serina-Treonina Quinases/genética , Ratos , Medula Espinal/efeitos dos fármacos , Medula Espinal/enzimologia , Medula Espinal/fisiopatologia
9.
Sci Total Environ ; 565: 519-528, 2016 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-27196989

RESUMO

Foshan is a major international ceramic center and the most polluted city in the Pearl River Delta (PRD). Here we present the results of the first long-term PM2.5 (particles <2.5µm) sampling and chemical characterization study of the city. A total of 2774 samples were collected at six sites from 2008 to 2014, and analyzed for water soluble species, elements and carbonaceous species. The major constituents of PM2.5 were sulfate, OC (Organic Carbon), nitrate, ammonium and EC (Elemental Carbon), which accounted for 50%-88% of PM2.5. PM2.5 and the most abundant chemical species decreased from 2008 to 2011, but rebounded in 2012-2013. After 2008, the chemical composition of PM2.5 changed dramatically due to the implementation of pollution control measures. From 2008 to 2011, SO4(2-) and NO3(-) were the two largest components; subsequently, however, OC was the largest component. The respective contributions of SO4(2-), NO3(-) and OC to the sum of water soluble species and carbonaceous species were 30.5%, 22.9% and 19.9% in 2008; and 20.2%, 16.5% and 30.2% in 2014. Distinct differences in nitrate and sulfate, and in mass ratio [NO3(-)]/[SO4(2-)] imply that mobile sources tended to more important in Foshan during 2012-2014. The results indicate that pollution control measures implemented during 2008-2014 had a large effect on anthropogenic elements (Pb, As, Cd, Zn and Cu) and water soluble species, but little influence on crustal elements (V, Mn, Ti, Ba and Fe) and carbonaceous species. The PMF method was used for source apportionment of PM2.5. Industry (including the ceramic industry and coal combustion), vehicles and dust were the three most important sources and comprised 39.2%, 20.0% and 18.4% of PM2.5 in 2008, respectively. However, secondary aerosols, vehicles and industry were the three most important sources and comprised 29.5%, 22.4% and 20.4% of PM2.5 in 2014, respectively. During the seven year study interval, the contributions of primary sources (industry and dust) decreased significantly, but secondary sources increased dramatically. Industry, dust and vehicles contributed 36.6µgm(-3), 13.9µgm(-3), and 9.2µgm(-3) to the reduction of PM2.5, respectively.


Assuntos
Poluição do Ar/análise , Material Particulado/química , Poluição do Ar/estatística & dados numéricos , China , Monitoramento Ambiental , Tamanho da Partícula
10.
Br J Nutr ; 115(9): 1509-20, 2016 05.
Artigo em Inglês | MEDLINE | ID: mdl-26983845

RESUMO

The aim of the present study was to assess the effects of dietary supplementation with epidermal growth factor (EGF)-expressing Saccharomyces cerevisiae on duodenal development in weaned piglets. In total, forty piglets weaned at 21-26 d of age were assigned to one of the five groups that were provided basic diet (control group) or diet supplemented with S. cerevisiae expressing either empty-vector (INVSc1(EV) group), tagged EGF (T-EGF) (INVSc1-TE(-) group), extracellular EGF (EE-EGF) (INVSc1-EE(+) group) or intracellular EGF (IE-EGF) (INVSc1-IE(+) group). All treatments were delivered as 60·00 µg/kg body weight EGF/d. On 0, 7, 14 and 21 d, eight piglets per treatment were sacrificed to analyse the morphology, activities and mRNA expressions of digestive enzymes, as well as Ig levels (IgA, IgM, IgG) in duodenal mucosa. The results showed significant improvement on 7, 14 and 21 d, with respect to average daily gain (P<0·05), mucosa morphology (villus height and crypt depth) (P<0·05), Ig levels (P<0·01), activities and mRNA expressions of digestive enzymes (creatine kinase, alkaline phosphatase, lactate dehydrogenase and sucrase) (P<0·05) and the mRNA expression of EGF-receptor (P<0·01) in NVSc1-TE(-), INVSc1-EE(+) and INVSc1-IE(+) groups compared with control and INVSc1(EV) groups. In addition, a trend was observed in which the INVSc1-IE(+) group showed an improvement in Ig levels (0·05

Assuntos
Suplementos Nutricionais , Duodeno/efeitos dos fármacos , Fator de Crescimento Epidérmico/farmacologia , Mucosa Intestinal/efeitos dos fármacos , Saccharomyces cerevisiae/metabolismo , Fosfatase Alcalina/genética , Fosfatase Alcalina/metabolismo , Animais , Creatina Quinase/genética , Creatina Quinase/metabolismo , Duodeno/crescimento & desenvolvimento , Duodeno/metabolismo , Fator de Crescimento Epidérmico/administração & dosagem , Receptores ErbB/genética , Receptores ErbB/metabolismo , Imunoglobulinas/metabolismo , Mucosa Intestinal/crescimento & desenvolvimento , Mucosa Intestinal/metabolismo , L-Lactato Desidrogenase/genética , L-Lactato Desidrogenase/metabolismo , Lactococcus lactis , RNA Mensageiro/metabolismo , Proteínas Recombinantes/administração & dosagem , Proteínas Recombinantes/farmacologia , Sacarase/genética , Sacarase/metabolismo , Suínos , Desmame
11.
ACS Med Chem Lett ; 7(3): 289-93, 2016 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-26985317

RESUMO

Positive allosteric modulators (PAMs) of the metabotropic glutamate receptor subtype 5 (mGluR5) are of interest due to their potential therapeutic utility in schizophrenia and other cognitive disorders. Herein we describe the discovery and optimization of a novel oxazolidinone-based chemotype to identify BMS-955829 (4), a compound with high functional PAM potency, excellent mGluR5 binding affinity, low glutamate fold shift, and high selectivity for the mGluR5 subtype. The low fold shift and absence of agonist activity proved critical in the identification of a molecule with an acceptable preclinical safety profile. Despite its low fold shift, 4 retained efficacy in set shifting and novel object recognition models in rodents.

12.
Biol Trace Elem Res ; 170(1): 216-23, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-26184120

RESUMO

The objective of this study was to investigate the effects of dietary supplemental chromium (Cr) on growth performance, carcass traits, and meat quality of broilers reared under heat stress. A total of 252 1-d-old Cobb 500 commercial female broilers were randomly allotted by body weight (BW) to one of six replicate cages (six broilers per cage) for each of seven treatments in a completely randomized design involving a 2 × 3 factorial arrangement of treatments with three Cr sources (Cr propionate, CrPro; Cr picolinate, CrPic; Cr chloride, CrCl3) and two concentrations of added Cr (0.4, or 2.0 mg of Cr/kg) plus a Cr-unsupplemented control group. Feed and distilled-deionized water were available ad libitum for an experimental phase of 42 days. For induction of heat stress, the house temperature was set at 33 ± 2 °C from 15 to 42 days of age. Results showed that birds supplemented with Cr, regardless of Cr source, had increased ADG (P = 0.032) than controls. Birds fed 2.0 mg Cr/kg diet had greater ADG (P = 0.005) than birds fed 0.4 mg Cr/kg diet. Compared to controls, birds fed with Cr had greater dressing percentage (P = 0.021). Percentage of abdominal fat decreased (P = 0.013), whereas, breast intramuscular fat (IMF) remained unaffected (P = 0.147) in Cr supplemented vs control broilers. Broilers supplemented Cr had decreased b* values of meat color (P = 0.042) in breast muscle. B*values were also lesser (P = 0.049) in birds fed CrPro than birds supplemented with CrCl3 or CrPic. Regardless of Cr source, the percentage of cooking loss was decreased (P = 0.025) with Cr supplementation in breast muscle when compared to controls. Results from this study indicate that Cr supplementation, independent of its source, could promote growth and improve carcass traits and meat quality of broilers under heat stress conditions. Chromium propionate seems to have greater beneficial effects on meat color in comparison with CrPic and CrCl3.


Assuntos
Galinhas/crescimento & desenvolvimento , Cromo/administração & dosagem , Resposta ao Choque Térmico , Animais , Feminino , Aves Domésticas
13.
Biol Trace Elem Res ; 169(2): 352-8, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-26123165

RESUMO

An experiment was conducted to investigate the effects of dietary chromium (Cr) source and concentration on growth performance, carcass traits, and some serum lipid parameters of broilers under normal rearing conditions for 42 days. A total of 252 1-day-old Cobb 500 commercial female broilers were randomly allotted by body weight (BW) to one of six replicate cages (six broilers per cage) for each of seven treatments in a completely randomized design involved in a 2 × 3 factorial arrangement of treatments with three Cr sources (Cr propionate (CrPro), Cr picolinate (CrPic), Cr chloride (CrCl3)) and two concentrations of added Cr (0.4 and 2.0 mg of Cr/kg) plus a Cr-unsupplemented control diet. The results showed that dietary Cr supplementation tended to increase the breast muscle percentage compared with the Cr-unsupplemented control group (P = 0.0784), while Cr from CrPic tended to have higher breast muscle percentage compared with Cr from CrCl3 (P = 0.0881). Chromium from CrPic also tended to increase the breast intramuscular fat (IMF) compared with Cr from CrCl3 (P = 0.0648). In addition, supplementation of 0.4 mg/kg Cr tended to decrease low-density lipoprotein cholesterol (LDL-C) (P = 0.0614). Compared with the control group, broilers fed Cr-supplemented diets had higher triglyceride (TG) (P = 0.0129) regardless of Cr source and Cr concentration. Chromium from CrPro and CrPic had lower total cholesterol (TC) compared with Cr from CrCl3 (P = 0.0220). These results indicate that dietary supplementation of Cr has effects on carcass characteristics and serum lipid parameters of broilers under normal rearing conditions, while supplementation of organic Cr can improve carcass characteristics and reduce the cholesterol content in serum.


Assuntos
Ração Animal/análise , Galinhas/crescimento & desenvolvimento , Cromo/farmacologia , Músculo Esquelético/efeitos dos fármacos , Gordura Abdominal/efeitos dos fármacos , Gordura Abdominal/crescimento & desenvolvimento , Animais , Galinhas/metabolismo , Cloretos/análise , Cloretos/farmacologia , Colesterol/sangue , Cromo/análise , Compostos de Cromo/análise , Compostos de Cromo/farmacologia , Suplementos Nutricionais , Fígado/efeitos dos fármacos , Fígado/crescimento & desenvolvimento , Músculo Esquelético/crescimento & desenvolvimento , Ácidos Picolínicos/análise , Ácidos Picolínicos/farmacologia , Propionatos/análise , Propionatos/farmacologia
14.
Appl Microbiol Biotechnol ; 99(17): 7125-35, 2015 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-25712680

RESUMO

Epidermal growth factor (EGF) ameliorates stress and prevents incomplete gastrointestinal development in early-weaned piglets in commercial swine farming. This study aimed to further analyze the biological activities of intracellularly expressed EGF (IE-EGF), extracellularly expressed EGF (EE-EGF), and tagged EGF (T-EGF) from Saccharomyces cerevisiae in early-weaned pigs. In this study, we assigned 24 pigs to each of 5 groups that were provided a basic diet (the control group) or a diet supplemented with empty vector-expressing S. cerevisiae [the INVSc1(EV) group], T-EGF-expressing S. cerevisiae [the INVSc1-TE(-) group], EE-EGF-expressing S. cerevisiae [the INVSc1-EE(+) group], or IE-EGF-expressing S. cerevisiae [the INVSc1-IE(+) group]. All treatments were delivered at a dose of 60 µg EGF/kg body weight (BW) everyday. All the piglets were sacrificed after 21 day to determine their physio-biochemical indexes, immune functions, and intestinal development. In the piglet experiments, recombinant S. cerevisiae survived throughout the intestinal tract. The BW and intestinal development (e.g., mean villous height, crypt depth, villous height:crypt depth ratio (IVR), and total protein, DNA, and RNA contents) of the piglets were significantly enhanced in the INVSc1-IE(+) group compared with the animals in the INVSc1-EE(+) and INVSc1-TE(-) groups (P < 0.05). In addition, increased proliferating cell nuclear antigen (PCNA) staining was observed in the piglets that received the INVSc1-IE(+) treatment (approximately 80 %) compared with those that received the INVSc1-TE(-) (approximately 70 %) and INVSc1-EE(+) treatments (approximately 70 %). The levels of lactate dehydrogenase (LDH), creatine kinase (CK), alkaline phosphatase (ALP), immunoglobulin A (IgA), immunoglobulin M (IgM), and immunoglobulin G (IgG) were also significantly increased in the INVSc1-IE(+) group compared with the INVSc1-EE(+) and INVSc1-TE(-) groups (P < 0.05). Furthermore, the proliferation of piglet enterocytes was also significantly stimulated by both IE-EGF and EE-EGF compared with T-EGF in vitro (P < 0.05). Our data further demonstrate the previously reported hypothesis that IE-EGF is more suitable than EE-EGF or T-EGF for applications in early-weaned pigs.


Assuntos
Dieta/métodos , Fator de Crescimento Epidérmico/administração & dosagem , Fator de Crescimento Epidérmico/metabolismo , Trato Gastrointestinal/microbiologia , Trato Gastrointestinal/fisiologia , Saccharomyces cerevisiae/metabolismo , Suínos/crescimento & desenvolvimento , Animais , Fator de Crescimento Epidérmico/genética , Saccharomyces cerevisiae/genética
15.
Virol Sin ; 30(1): 45-51, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25680444

RESUMO

The novel phage lysin PlySs2, is reported to be highly active against various bacteria, including staphylococci, streptococci and Listeria. However, the molecular mechanisms underlying its broad lytic spectrum remain to be established. In the present study, the lytic activity of the catalytic domain (CD, PlySc) and binding specificity of the cell wall binding domain (CBD, PlySb) of PlySs2 were examined. Our results showed that PlySc alone maintains very limited lytic activity. Enhanced green fluorescent protein (EGFP)-fused PlySb displayed high binding affinity to the streptococcal strains tested, including S. suis, S. dysgalactiae, and S. agalactiae, but not staphylococci, supporting its utility as a good CBD donor for streptococcal-targeted lysin engineering. EGFP-fused intact PlySs2 similarly displayed high affinity for streptococci, but not staphylococci. Notably, four truncated PlySb fragments showed no binding capacity. These findings collectively indicate that integrity of the PlySc and PlySb domains is an essential determinant of the broad lytic activity of PlySs2.


Assuntos
Bacteriófagos/metabolismo , Parede Celular/virologia , Streptococcus/virologia , Proteínas Virais/metabolismo , Bacteriófagos/química , Bacteriófagos/genética , Especificidade de Hospedeiro , Estrutura Terciária de Proteína , Proteínas Virais/química , Proteínas Virais/genética
16.
Microb Biotechnol ; 8(2): 210-20, 2015 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-25219798

RESUMO

Developing chimeric lysins with a wide lytic spectrum would be important for treating some infections caused by multiple pathogenic bacteria. In the present work, a novel chimeric lysin (Ply187N-V12C) was constructed by fusing the catalytic domain (Ply187N) of the bacteriophage lysin Ply187 with the cell binding domain (146-314aa, V12C) of the lysin PlyV12. The results showed that the chimeric lysin Ply187N-V12C had not only lytic activity similar to Ply187N against staphylococcal strains but also extended its lytic activity to streptococci and enterococci, such as Streptococcus dysgalactiae, Streptococcus agalactiae, Streptococcus pyogenes, Enterococcus faecium and Enterococcus faecalis, which Ply187N could not lyse. Our work demonstrated that generating novel chimeric lysins with an extended lytic spectrum was feasible through fusing a catalytic domain with a cell-binding domain from lysins with lytic spectra across multiple genera.


Assuntos
Bacteriólise , Enterococcus/efeitos dos fármacos , Mucoproteínas/metabolismo , Proteínas Recombinantes de Fusão/metabolismo , Staphylococcus/efeitos dos fármacos , Streptococcus/efeitos dos fármacos , Proteínas Virais/metabolismo , Domínio Catalítico/genética , Mucoproteínas/genética , Ligação Proteica , Proteínas Recombinantes de Fusão/genética , Especificidade por Substrato , Proteínas Virais/genética
17.
World J Gastroenterol ; 20(40): 14950-7, 2014 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-25356056

RESUMO

AIM: To explore the therapeutic role of globular adiponectin (gAd) in high-fat diet/streptozotocin (STZ)-induced type 2 diabetic rats with nonalcoholic fatty liver disease (NAFLD). METHODS: Seven rats were fed a basic diet (normal control group; NC) during the experiment. Experimental rats (14 rats) were given a high-fat diet for 4 wk and were then injected with STZ to induce type 2 diabetes mellitus (T2DM) and NAFLD. Half of the T2DM/NAFLD rats were randomly injected intraperitoneally with gAd for 7 d (gAd-treated group), while the other 7 rats (T2DM/NAFLD group) received 0.9% saline. Plasma biochemical parameters and insulin concentrations were measured. Liver histopathology was examined by hematoxylin-eosin staining. Insulin receptor expression in the liver was analyzed by immunohistochemical staining, Western blot and quantitative real-time reverse transcription polymerase chain reaction analysis. RESULTS: Compared to the control group, the T2DM/NAFLD group had increased levels of glucolipid and decreased levels of insulin. Plasma glucose and lipid levels were decreased in the gAd-treated group, while serum insulin levels increased. The expression of insulin receptor in the T2DM/NAFLD group increased compared with the NC group, and gAd downregulated insulin receptor expression in the livers of T2DM/NAFLD rats. Steatosis of the liver was alleviated in the gAd-treated group compared to the T2DM/NAFLD group (NAS 1.39 ± 0.51 vs 1.92 ± 0.51, P < 0.05). CONCLUSION: Globular adiponectin exerts beneficial effects in T2DM rats with NAFLD by promoting insulin secretion, mediating glucolipid metabolism, regulating insulin receptor expression and alleviating hepatic steatosis.


Assuntos
Adiponectina/farmacologia , Diabetes Mellitus Experimental/tratamento farmacológico , Diabetes Mellitus Tipo 2/tratamento farmacológico , Hipoglicemiantes/farmacologia , Fígado/efeitos dos fármacos , Hepatopatia Gordurosa não Alcoólica/tratamento farmacológico , Adiponectina/administração & dosagem , Animais , Biomarcadores/sangue , Glicemia/metabolismo , Diabetes Mellitus Experimental/sangue , Diabetes Mellitus Experimental/induzido quimicamente , Diabetes Mellitus Experimental/genética , Diabetes Mellitus Tipo 2/sangue , Diabetes Mellitus Tipo 2/induzido quimicamente , Diabetes Mellitus Tipo 2/genética , Dieta Hiperlipídica , Hipoglicemiantes/administração & dosagem , Injeções Intraperitoneais , Insulina/sangue , Lipídeos/sangue , Fígado/metabolismo , Fígado/patologia , Masculino , Hepatopatia Gordurosa não Alcoólica/sangue , Hepatopatia Gordurosa não Alcoólica/etiologia , Hepatopatia Gordurosa não Alcoólica/genética , Hepatopatia Gordurosa não Alcoólica/patologia , RNA Mensageiro/metabolismo , Ratos Wistar , Receptor de Insulina/efeitos dos fármacos , Receptor de Insulina/genética , Receptor de Insulina/metabolismo , Estreptozocina
18.
Guang Pu Xue Yu Guang Pu Fen Xi ; 34(5): 1412-5, 2014 May.
Artigo em Chinês | MEDLINE | ID: mdl-25095449

RESUMO

A new KMnO4-MnO2 solid multisorbent tube for capturing mercury in workplace air was developed. Experimental conditions for solid multisorbent tube, efficiency of sampling, desorption efficiency and stability were studied. Mercury and its compounds in air were captured by solid KMnO4-MnO2 sorbent filled tube and desorbed with 0. 90 mol L-1 sulfuric acid solution. Mercury and its compounds were quantitatively analyzed according to the method of GBZ/T 160. 14-2004 cold vapor atomic absorption spectrometry. The linear range of the proposed method was 0. 000 2-0. 015 0 mg L-1 with r=0. 999 1, the average efficiency of sampling was 99. 9%-100. 0% in the concentration range of 0. 001-2. 820 mg m-3 , and the breakthrough capacity was more than 505.4 microg for 300 mg KMnO4-MnO2 solid multisorbent, the average recovery rate was 96. 4% approximately103. 8%, the intra-day and inter-day precision was 3. 0% approximately 3. 3% and 3. 5% approximately 5. 2% respectively, the limit of detection was 0. 0013 mg m-3 (7. 5 L of air ) and 0. 000 6 mg m-3 (96 L of air), after sampling, and the solid multisorbent tube could be kept at least 30 d at room temperature without significant loss. The present method was simple and suitable for capturing mercury and its compounds in the workplace air and ambient air. The solid multisorbent tube was useful for personal sampling and time weighted average sampling.


Assuntos
Poluentes Ocupacionais do Ar/análise , Mercúrio/análise , Espectrofotometria Atômica , Local de Trabalho , Temperatura Baixa , Monitoramento Ambiental , Gases
19.
Exp Ther Med ; 8(2): 355-362, 2014 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-25009583

RESUMO

The aim of the present study was to detect the elastic modulus (stiffness) of the livers of rabbits with non-alcoholic and alcoholic fatty liver disease using real-time shear wave elastography (SWE), and to investigate the fibrosis development process in the formation of fatty liver. The stiffness of the fatty livers in rabbit models prepared via feeding with alcohol or a high-fat diet were measured using a real-time SWE ultrasound system and a 4-15-MHz linear array probe, and the liver stiffness was compared with the pathological staging of the disease. The stiffness of the liver was positively correlated with the degree of pathological change in fatty liver disease (P<0.01). The stiffness of the liver in the alcoholic fatty liver group was higher compared with that in the non-alcoholic fatty liver and control groups, and the stiffness in the non-alcoholic fatty liver group was higher than that in the control group (P<0.01). Real-time SWE objectively identified the trend in the changing stiffness of the liver and noninvasively detected the development of fibrosis in the progression of non-alcoholic and alcoholic fatty liver disease.

20.
Biofouling ; 30(6): 667-74, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24742201

RESUMO

Methicillin-resistant Staphylococcus aureus (MRSA) is responsible for a large number of chronic infections due to its ability to form robust biofilms. Herein, the authors evaluated the anti-biofilm activity of a Staphylococcus specific chimeric lysin ClyH on MRSA biofilms. ClyH is known to be active against planktonic MRSA cells in vitro and in vivo. The minimum concentrations for biofilm eradication (MCBE) of ClyH were 6.2-50 mg l(-1), much lower than those of antibiotics. Scanning electron microscope (SEM) analysis revealed that ClyH eliminated MRSA biofilms through cell lytic activity in a time-dependent manner. Viable plate counts and kinetic analysis demonstrated that biofilms of different ages displayed varying susceptibility to ClyH. Together with previously demonstrated in vivo efficacy of ClyH against MRSA, the degradation efficacy against biofilms of different ages indicates that ClyH could be used to remove MRSA biofilms in vivo.


Assuntos
Biofilmes/efeitos dos fármacos , Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos , Mucoproteínas/farmacologia , Proteínas Recombinantes de Fusão/farmacologia , Análise de Variância , Relação Dose-Resposta a Droga , Violeta Genciana , Staphylococcus aureus Resistente à Meticilina/ultraestrutura , Microscopia Eletrônica de Varredura
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