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1.
Artif Cells Nanomed Biotechnol ; 48(1): 8-14, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31852248

RESUMO

Osteoarthritis is a common type of degenerative joint disease. Inflammation-related chondrocyte senescence plays a major role in the pathogenesis of osteoarthritis. Omentin-1 is a newly identified anti-inflammatory adipokine involved in lipid metabolism. In this study, we examined the biological function of omentin-1 in cultured chondrocytes. The presence of omentin-1 potently suppresses IL-1ß-induced cellular senescence as revealed by staining with senescence-associated beta-galactosidase (SA-ß-Gal). At the cellular level, omentin-1 attenuates IL-1ß-induced G1 phase cell-cycle arrest. Mechanistically, we demonstrate that omentin-1 reduced IL-1ß-induced expression of senescent factors including caveolin-1, p21, and PAI-1 as well as p53 acetylation through ameliorating SIRT1 reduction. Notably, silencing of SIRT1 abolishes IL-1ß-induced senescence along with the induction of p21 and PAI-1, suggesting that the action of omentin-1 in chondrocytes is dependent on SIRT1. Collectively, our results revealed the molecular mechanism through which the adipokine omentin-1 exerts a beneficial effect, thereby protecting chondrocytes from senescence. Thus, omentin-1 could have clinical implication in the treatment of osteoarthritis.

2.
Phys Rev E ; 96(2-1): 023105, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28950509

RESUMO

We investigate fluctuations of the velocity and temperature fields in two-dimensional (2D) Rayleigh-Bénard (RB) convection by means of direct numerical simulations (DNS) over the Rayleigh number range 10^{6}≤Ra≤10^{10} and for a fixed Prandtl number Pr=5.3 and aspect ratio Γ=1. Our results show that there exists a counter-gradient turbulent transport of energy from fluctuations to the mean flow both locally and globally, implying that the Reynolds stress is one of the driving mechanisms of the large-scale circulation in 2D turbulent RB convection besides the buoyancy of thermal plumes. We also find that the viscous boundary layer (BL) thicknesses near the horizontal conducting plates and near the vertical sidewalls, δ_{u} and δ_{v}, are almost the same for a given Ra, and they scale with the Rayleigh and Reynolds numbers as ∼Ra^{-0.26±0.03} and ∼Re^{-0.43±0.04}. Furthermore, the thermal BL thickness δ_{θ} defined based on the root-mean-square (rms) temperature profiles is found to agree with Prandtl-Blasius predictions from the scaling point of view. In addition, the probability density functions of turbulent energy ɛ_{u^{'}} and thermal ɛ_{θ^{'}} dissipation rates, calculated, respectively, within the viscous and thermal BLs, are found to be always non-log-normal and obey approximately a Bramwell-Holdsworth-Pinton distribution first introduced to characterize rare fluctuations in a confined turbulent flow and critical phenomena.

3.
Zhonghua Nan Ke Xue ; 19(10): 896-8, 2013 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-24218942

RESUMO

OBJECTIVE: To investigate the effects of hepatitis B virus (HBV) in semen on human semen parameters and sperm DNA integrity. METHODS: We detected HBV DNA in the semen samples of 153 HBsAg-seropositive patients by real-time fluorescence quantitative PCR and calculated the sperm nuclear DNA fragmentation index (DFI) by sperm chromatin dispersion (SCD) assay. We compared the semen parameters between the HBV DNA-positive group (A, n = 43) and HBV DNA-negative group (B, n = 110) and analyzed the correlation of sperm DFI with the number of HBV DNA copies in the semen. RESULTS: HBV DNA was detected in 43 (28.1%) of the 153 semen samples. No statistically significant differences were observed in age, semen volume and sperm concentration between groups A and B (P >0.05). Compared with group B, group A showed significantly decreased sperm viability ([58.0 +/- 18.8]% vs [51.4 +/-17.1]%, P<0.05), progressively motile sperm ([29.6 +/- 13.3]% vs [24.5 +/- 10.1]%, P<0.05), average straight-line velocity ([23.7 +/- 4.0] microm/s vs [19.9 +/- 4.5 ] microm/s, P<0.01) and average path velocity ([26.5 +/- 7.0] microm/s vs [23.4 +/- 5.3] microm/s, P<0.01), but remarkably decreased sperm DFI ([19.3 +/- 8.0]% vs [24.2 +/- 9.4]%, P<0.01). The number of HBV DNA copies in semen exhibited a significant positive correlation with sperm DFI (r = 0.819, P < 0.01). CONCLUSION: HBV DNA in semen is not significantly associated with the number of sperm, but may affect sperm viability, velocity and DFI. There is a load-effect relationship between the number of HBV DNA copies in semen and sperm nuclear DNA integrity.


Assuntos
DNA Viral/isolamento & purificação , Antígenos de Superfície da Hepatite B/sangue , Vírus da Hepatite B/genética , Espermatozoides/virologia , Adulto , Fragmentação do DNA , Humanos , Masculino , Análise do Sêmen , Contagem de Espermatozoides
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