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1.
Zhongguo Zhong Yao Za Zhi ; 44(21): 4634-4640, 2019 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-31872658

RESUMO

There is no consensus on the drying methods of Salvia miltiorrhiza in ancient and modern times,especially on the content of phenolic acid in fresh S. miltiorrhiza. In order to further explore the content of main components in fresh S. miltiorrhiza and study the dynamic changes during the drying process,the content of main components was used as the index in this study to evaluate the processing method,drying method,correlation between dehydration rate and component content for fresh S. miltiorrhiza. In addition,the sealed and unsealed parallel control groups were set to carry out verification test during the drying process. UPLC method was used for determination of seven main components including rosmarinic acid,lithosperic acid,salvianolic acid B,cryptotanshinone,tanshinoneⅠ,methylene salianolate and tanshinone ⅡAin S. miltiorrhiza. The results showed that the fresh S. miltiorrhiza contained low levels of phenolic acid,and the content of phenolic acid increased significantly with the increase of dehydration rate during drying process,while the change of tanshinone was not obvious. In the comparison of three drying methods,we found that drying at 50 ℃ was better than drying in the sun,and drying in the sun was superior to drying in the shade. So,drying at 50 ℃ was the best drying method. The correlation between dehydration and phenolic acid content of S. miltiorrhiza was analyzed by verification test and SPSS software,which further proved that the dehydration rate was significantly positively correlated with the content of phenolic acid components. This study provides reference for the production processing and drying methods of S. miltiorrhiza medicinal materials,which is of great significance for improving the quality of S. miltiorrhiza.


Assuntos
Salvia miltiorrhiza , Dessecação , Raízes de Plantas
2.
Zhong Yao Cai ; 38(6): 1212-6, 2015 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-26762063

RESUMO

OBJECTIVE: To identify Chuanxiong Rhizoma,Angelicae Sinensis Radix and Ligustici Rhizoma et Radix by establishing the HPLC specific chromatograms of their volatile oil and to compare their specific peaks. METHODS: The HPLC method used methanol-water as mobile phase. Their specific peaks were analysed by HPLC-MS. RESULTS: Under the selected spectrum condition, their HPLC specific chromatograms were established. Senkyunolide A, butylphalide, coniferylferulate, E-ligustilide, Z-ligustilide, neocnidilide and E-butylidenephthalide were identified as specific peaks in chromatograms based on their MS data. CONCLUSION: This method is simple, accurate and available to identify Chuanxiong Rhizoma, Angelicae Sinensis Radix and Ligustici Rhizoma et Radix. It provides reference for quality control of their medicinal materials and Chinese Patent Medicine.


Assuntos
Angelica sinensis/química , Medicamentos de Ervas Chinesas/química , Ligusticum/química , Óleos Voláteis/química , Óleos Vegetais/química , 4-Butirolactona/análogos & derivados , Benzofuranos , Cromatografia Líquida de Alta Pressão , Anidridos Ftálicos , Raízes de Plantas/química , Rizoma/química
3.
Zhongguo Zhong Yao Za Zhi ; 39(24): 4798-803, 2014 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-25898581

RESUMO

To study the effect of steaming and baking process on contents of alkaloids in Aconite Lateralis Radix (Fuzi), 13 alkaloids were analyzed by UPLC-MS/MS equipped with ESI ion source in MRM mode. In steaming process, the contents of diester-diterpenoid alkaloids decreased rapidly, the contents of monoester-diterpenoid alkaloids firstly increased, reached the peak at 40 min, and then deceased gradually. The contents of aconine alkaloids (mesaconine, aconine and hypaconine) increased all the time during processing, while the contents of fuziline, songorine, karacoline, salsolionl were stable or slightly decreased. In baking process, dynamic variations of alkaloids were different from that in the steaming process. Diester-diterpenoid alkaloids were degraded slightly slower than in steaming process. Monoester-diterpenoid alkaloids, aconine alkaloids and the total alkaloids had been destroyed at different degrees, their contents were significantly lower than the ones in steaming Fuzi at the same processing time. This experiment revealed the dynamic variations of alkaloids in the course of steaming and baking. Two processing methods which can both effectively remove the toxic ingredients and retain the active ingredients are simple and controllable, and are valuable for popularization and application.


Assuntos
Aconitum/química , Alcaloides/isolamento & purificação , Medicamentos de Ervas Chinesas/isolamento & purificação , Extratos Vegetais/isolamento & purificação , Aconitina/análogos & derivados , Aconitina/isolamento & purificação , Cromatografia Líquida de Alta Pressão , Estabilidade de Medicamentos , Temperatura Alta , Vapor , Espectrometria de Massas em Tandem , Fatores de Tempo
4.
Zhonghua Yi Xue Za Zhi ; 93(19): 1499-502, 2013 May 21.
Artigo em Chinês | MEDLINE | ID: mdl-24029577

RESUMO

OBJECTIVE: To explore the inhibitory effects of human umbilical cord-derived mesenchymal stem cells (hUCMSC) on the proliferation of peripheral blood mononuclear cells (PBMC) from spondyloarthritis (SpA) patients. METHODS: A total of 12 SpA patients at Chinese PLA General Hospital were recruited from May 2012 to October 2012. Information on demographic characteristics, disease and functional activity was collected. Isolated PBMC were stimulated by phytohemagglutinin (PHA, 1 µg/ml) in the presence or absence of hUCMSC.The proliferation of hUCMSC was suppressed by irradiation with Co60 (30 Gy) before co-culturing with PBMC. The proliferation of PBMC was determined by Cell Counting Kit-8 (CCK-8). Cell cycle profiles of PBMC were analyzed by flow cytometry. The association of inhibitory effect of hUCMSC with the disease and functional activity of SpA patients was examined. RESULTS: After coculturing with hUCMSC by cell-to-cell contact for 5 days, the proliferation of PBMC stimulated by PHA (1 µg/ml) was significantly inhibited by hUCMSC in a dose-dependent manner.The inhibition rate of the proliferation of PBMC cocultured with hUCMSC by cell-to-cell contact was higher than that by Transwell culture (57% ± 17% vs 32% ± 12%, P < 0.01). Compared to PBMC cultured alone, a larger number of PBMC cocultured with hUCMSC were in phase G1 (86% ± 3% vs 68% ± 5%, P < 0.01) while a lower number of cells in phases S and G2 (8% ± 3% vs 26% ± 5%, P < 0.01). No association was found between the inhibitory effect of hUCMSC and the disease and functional activity. CONCLUSION: The proliferation of PBMC from SpA patients may be inhibited by hUCMSC. And hUCMSC have therapeutic potentials for SpA patients.


Assuntos
Proliferação de Células , Leucócitos Mononucleares/citologia , Células-Tronco Mesenquimais/citologia , Espondilartrite/patologia , Adulto , Ciclo Celular , Células Cultivadas , Técnicas de Cocultura , Feminino , Humanos , Masculino , Cordão Umbilical/citologia
5.
Zhonghua Yi Xue Za Zhi ; 93(13): 966-9, 2013 Apr 02.
Artigo em Chinês | MEDLINE | ID: mdl-23886255

RESUMO

OBJECTIVE: To evaluate the effects of duloxetine on depression, anxiety, pain, disease activity and function in patients with ankylosing spondylitis (AS). METHODS: A total of 55 AS patients with concurrent depression disorders were randomized into treatment and control groups. Both were given conventional therapy of AS while duloxetine was administered in treatment group. Bath ankylosing spondylitis disease activity index (BASDAI), functional Index (BASFI) and metrology Index (BASMI), spinal pain, self-rating anxiety scale (SAS), self-rating depression scale (SDS) and Hamilton depression scale (HAMD) were recorded before and Weeks 4 and 8 weeks post-treatment. RESULTS: (1) Spinal pain, BASDAI, BASFI and SDS scores significantly declined by repeated measurement data analysis of variance in treatment group (P < 0.05). And no statistical difference existed between BASMI and SAS (P > 0.05). (2) The reduced rates of spinal pain and BASDAI were positively correlated with those of SDS and SAS. And the reduced rate of BASFI was positively correlated with SDS and HAMD reduced rate. However, no relationship existed between BASMI, SDS, SAS or HAMD. (3) The remission rate of AS symptoms and depression disorders were both significantly higher in treatment group than that in control group (P < 0.01, P < 0.05). CONCLUSION: The combined regimen of duloxetine and conventional therapy is significantly effective in the treatment of AS patients with depression and anxiety.


Assuntos
Antidepressivos/uso terapêutico , Ansiedade/tratamento farmacológico , Depressão/tratamento farmacológico , Espondilite Anquilosante/tratamento farmacológico , Espondilite Anquilosante/psicologia , Tiofenos/uso terapêutico , Adulto , Analgésicos/uso terapêutico , Ansiedade/etiologia , Depressão/etiologia , Cloridrato de Duloxetina , Feminino , Humanos , Masculino , Dor/tratamento farmacológico , Dor/etiologia , Espondilite Anquilosante/complicações , Adulto Jovem
6.
Zhonghua Yi Xue Za Zhi ; 93(13): 970-2, 2013 Apr 02.
Artigo em Chinês | MEDLINE | ID: mdl-23886256

RESUMO

OBJECTIVE: To evaluate the relationship between sleep quality and nocturnal pain in ankylosing spondylitis (AS) patients. METHODS: A total of 157 AS patients were recruited. Pittsburgh sleeping quality index (PSQI) and visual analogue scale (VAS) were used to assess sleep quality and nocturnal pain respectively. Disease activity was assessed by Bath ankylosing spondylitis disease activity index (BASDAI), C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR). RESULTS: The mean PSQI and nocturnal pain score was 6.6 ± 3.6 and 3.7 ± 3.0 respectively. Fifty-five of them had poor sleep. Quality of sleep was positively correlated with nocturnal pain (P < 0.01). Elevated ESR/CRP was found in 47.1% (74/157) of them. Patients with elevated ESR/CRP had significantly a higher level of BASDAI than those with normal ESR/CRP (P < 0.01). Logistic regression analysis revealed that nocturnal pain was most powerful risk factor of sleep disturbances (P < 0.05). In hierarchical multiple regression analysis, the quality of sleep variable contributed significantly to the variance in nocturnal pain scores, adding an additional 18.2% to the overall R-square beyond that accounted by demographic and disease-related variables (inclusion of ESR and CRP) (R(2) = 0.175). CONCLUSION: Sleep quality has close relationship with nocturnal pain. And sleep disturbances should be considered in the management of AS.


Assuntos
Dor/etiologia , Transtornos do Sono-Vigília/etiologia , Espondilite Anquilosante/psicologia , Adolescente , Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Medição da Dor , Espondilite Anquilosante/complicações , Inquéritos e Questionários , Adulto Jovem
7.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 21(2): 455-9, 2013 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-23628053

RESUMO

In this study, the inhibitory effect of human umbilical cord-derived mesenchymal stem cells (hUCMSC) on interleukin-17 (IL-17) production in peripheral blood T cells from patients with spondyloarthritis (SpA) were investigated, in order to explore the therapeutic potential of hUCMSC in the SpA. Peripheral blood mononuclear cells (PBMNC) were isolated from patients with SpA (n = 12) and healthy subjects (n = 6). PBMNC were cultured in vitro with hUCMSC or alone. The expression of IL-17 in CD4(+) T cells or γ/δ T cells were determined in each subject group by flow cytometry. IL-17 concentrations in PBMNC culture supernatants were measured by ELISA. The results indicated that the proportion of IL-17-producing CD4(+) T cells and IL-17-producing γ/δ T cells of SpA patients were 4.5 folds and 5 folds of healthy controls [CD3(+)CD4(+)IL-17(+) cells (3.42 ± 0.82)% vs (0.75 ± 0.25)%, P < 0.01; CD3(+)γδTCR(+)IL-17(+) cells (0.30 ± 0.10)% vs (0.06 ± 0.02)%, P < 0.01]. After co-culture of PBMNC in patients with hUCMSC, the increased proportions of CD3(+)CD4(+)IL-17(+) cells and CD3(+)γδTCR(+)IL-17(+) cells in SpA patients were inhibited significantly by hUCMSC [CD3(+)CD4(+)IL-17(+) cells (3.42 ± 0.82)% vs (1.81 ± 0.59)% (P < 0.01); CD3(+)γδTCR(+)IL-17(+) cells (0.30 ± 0.10)% vs (0.16 ± 0.06)% (P < 0.01]. In response to phytohemagglutinin (PHA, 1 µg/ml), PBMNC from SpA patients secreted more IL-17 than that from healthy control [(573.95 ± 171.68) pg/ml vs (115.53 ± 40.41) pg/ml (P < 0.01)]. In the presence of hUCMSC, PBMNC of SpA patients produced less amount of IL-17 [(573.95 ± 171.68) pg/ml vs (443.20 ± 147.94) pg/ml, (P < 0.01)]. It is concluded that the IL-17 production in peripheral blood T cells from SpA patients can be inhibited by hUCMSC, which have therapeutic potential for SpA.


Assuntos
Interleucina-17/metabolismo , Células-Tronco Mesenquimais , Espondilartrite/sangue , Linfócitos T/metabolismo , Humanos , Leucócitos Mononucleares/citologia , Contagem de Linfócitos , Espondilartrite/metabolismo , Espondilartrite/terapia , Cordão Umbilical/citologia
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