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1.
J Pharm Anal ; 11(5): 653-660, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34765279

RESUMO

A new electrochemical sensor for organophosphate pesticide (methyl-paraoxon) detection based on bifunctional cerium oxide (CeO2) nanozyme is here reported for the first time. Methyl-paraoxon was degraded into p-nitrophenol by using CeO2 with phosphatase mimicking activity. The CeO2 nanozyme-modified electrode was then synthesized to detect p-nitrophenol. Cyclic voltammetry was applied to investigate the electrochemical behavior of the modified electrode, which indicates that the signal enhancement effect may attribute to the coating of CeO2 nanozyme. The current research also studied and discussed the main parameters affecting the analytical signal, including accumulation potential, accumulation time, and pH. Under the optimum conditions, the present method provided a wider linear range from 0.1 to 100 µmol/L for methyl-paraoxon with a detection limit of 0.06 µmol/L. To validate the proof of concept, the electrochemical sensor was then successfully applied for the determination of methyl-paraoxon in three herb samples, i.e., Coix lacryma-jobi, Adenophora stricta and Semen nelumbinis. Our findings may provide new insights into the application of bifunctional nanozyme in electrochemical detection of organophosphorus pesticide.

2.
Inflamm Res ; 70(9): 959-969, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34390377

RESUMO

BACKGROUND: Donor cell-derived exosomes regulate recipient cell functions. The aim of this study was to investigate the effect of human normal bladder stromal cell (hBSC) derived exosomal miR-217 on bladder cell cancer proliferation and migration. METHODS: Human BSCs were transfected with miR-217 mimic or inhibitor and hBSC-derived exosomes were isolated. Human bladder cancer cell lines (T24 and 5367) were co-cultured with hBSC-derived exosomal miR-217 mimic or inhibitor. Proliferation, migration, and apoptosis of the bladder cancer cells were assessed by Edu assay, Transwell migration assay, and Annexin V assay. RESULTS: Expression of miR-217 was significantly higher in the T24 and 5367 cell lines (P < 0.01). Exosomal miR-217 mimic enhanced proliferation and migration of T24 and 5367 cells, but inhibited apoptosis of the cells (P < 0.01); in contrast, exosomal miR-217 inhibitor suppressed proliferation and migration but stimulated apoptosis of the two cancer cell lines (P < 0.01). Moreover, exosomal miR-217 mimic stimulated YAP and its target proteins including Cyr61, CTGF, and ANKRD1 (P < 0.01), and in contrast, exosomal miR-217 inhibitor suppressed YAP and its target proteins (P < 0.01). CONCLUSION: These findings suggested that hBSC-derived exosomal miR-217 may act as oncogene in bladder cancer cells, and that Hippo-YAP signaling pathway maybe the target for miR-217 in the bladder cancer cell lines.

3.
ACS Appl Mater Interfaces ; 13(27): 31624-31634, 2021 Jul 14.
Artigo em Inglês | MEDLINE | ID: mdl-34219452

RESUMO

Solar steam generation is considered as an efficient way for addressing water shortage issues via seawater desalination and wastewater purification. In a solar evaporator, an absorber would convert optical energy to heat for evaporating nearby water. In this process, many low-boiling-point contaminants can also be evaporated along with water steam, which compromises the effectiveness of purification. There is, so far, no study on the removal of such low-boiling-point contaminants such as organic pesticides in wastewater. To address this problem, we demonstrate a versatile carbon hybrid aerogel (CHA) as a solar powered water purification platform. With an elaborate absorber design, the maximum solar evaporation rate of 2.1 kg m-2 h-1 is achieved under 1 sun illumination. More importantly, CHA can effectively suppress the evaporation of low-boiling-point contaminants including common pesticides and mercury ion via its strong adsorption and retention effect. Synergetic steaming and the adsorption of CHA will inspire more paradigms of solar steam generation technologies for applications relevant to detoxification and water remediation.

4.
Small ; 17(43): e2101487, 2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-34151518

RESUMO

As a sustainable and clean water production technology, solar thermal water evaporation has been extensively studied in the past few years. One challenge is that upon operation, salt would form on surface of the solar absorbers leading to inefficient water supply and light absorption and thus much reduced water vaporization rate. To address this problem, a simple solar evaporator based on an array of aligned millineedles for efficient solar water evaporation and controlled site-specific salt formation is demonstrated. The maximum solar evaporation rate achieved is 2.94 kg m-2 h-1 under one Sun irradiation in brine of high salinity (25 wt% NaCl), achieving energy conversion efficiency of 94.5% simultaneously. More importantly, the spontaneously site-specific salt formation on the tips of millineedles endows this solar evaporator with salt harvesting capacity. Rationally separating the clean water and salt from brine by condensation and gravity assistance, this tip-preferential crystallization solar evaporator is not affected by the salt clogging compared with conventional 2D solar evaporators. This study provides new insights on the design of solar evaporators and advances their applications in sustainable seawater desalination and wastewater management.


Assuntos
Energia Solar , Purificação da Água , Água do Mar , Cloreto de Sódio , Luz Solar
5.
Nanoscale ; 13(17): 8012-8016, 2021 May 06.
Artigo em Inglês | MEDLINE | ID: mdl-33884397

RESUMO

While photodynamic therapy (PDT) of cancer has attracted much recent attention, its general applications are limited by the shallow tissue penetration depth of short-wavelength photons and the low oxygen contents in typical solid tumors. Herein, we develop small molecule (BthB)-based nanoparticles (NPs) which not only generate heat for effective photothermal therapy (PTT), but also generate superoxide radicals (O2˙-) for hypoxia-overcoming photodynamic therapy (PDT) upon irradiation with an 808 nm laser. To the best of our knowledge, there are few reports of organic PDT agents which can work in hypoxia upon irradiation with photons having wavelengths longer than 800 nm. With the merits of NIR-excitability for better penetration depth, the BthB NPs are demonstrated both in vitro and in vivo to be highly effective for cancer ablation.


Assuntos
Nanopartículas , Neoplasias , Fotoquimioterapia , Humanos , Hipóxia , Nanomedicina , Neoplasias/tratamento farmacológico , Fármacos Fotossensibilizantes/uso terapêutico , Oxigênio Singlete , Superóxidos
6.
Angew Chem Int Ed Engl ; 60(21): 11758-11762, 2021 05 17.
Artigo em Inglês | MEDLINE | ID: mdl-33724623

RESUMO

Extensive recent efforts have been put on the design of high-performance organic near-infrared (NIR) photothermal agents (PTAs), especially over NIR-II bio-window (1000-1350 nm). So far, the development is mainly limited by the rarity of molecules with good NIR-II response. Here, we report organic nanoparticles of intermolecular charge-transfer complexes (CTCs) with easily programmable optical absorption. By employing different common donor and acceptor molecules to form CTC nanoparticles (CT NPs), absorption peaks of CT NPs can be controllably tuned from the NIR-I to NIR-II region. Notably, CT NPs formed with perylene and TCNQ have a considerably red-shifted absorption peak at 1040 nm and achieves a good photothermal conversion efficiency of 42 % under 1064 nm excitation. These nanoparticles were used for antibacterial application with effective activity towards both Gram-negative and Gram-positive bacteria. This work opens a new avenue into the development of efficient PTAs.


Assuntos
Antibacterianos/farmacologia , Nanopartículas/química , Antibacterianos/química , Antibacterianos/efeitos da radiação , Derivados de Benzeno/química , Derivados de Benzeno/farmacologia , Derivados de Benzeno/efeitos da radiação , Escherichia coli/efeitos dos fármacos , Raios Infravermelhos , Testes de Sensibilidade Microbiana , Nanopartículas/efeitos da radiação , Nitrilas/química , Nitrilas/farmacologia , Nitrilas/efeitos da radiação , Perileno/química , Perileno/farmacologia , Perileno/efeitos da radiação , Compostos Policíclicos/química , Compostos Policíclicos/farmacologia , Compostos Policíclicos/efeitos da radiação , Solubilidade , Staphylococcus aureus/efeitos dos fármacos , Eletricidade Estática/efeitos adversos , Compostos de Sulfidrila/química , Compostos de Sulfidrila/farmacologia , Compostos de Sulfidrila/efeitos da radiação , Água/química
7.
ACS Appl Mater Interfaces ; 13(14): 15983-15991, 2021 Apr 14.
Artigo em Inglês | MEDLINE | ID: mdl-33788531

RESUMO

Organic small molecule-based phototheranostics hold great promise for clinical translation by virtue of their distinct chemical structure, easy reproducibility, and high purity. However, reported molecular agents typically have relatively low optical absorbances, particularly over the near-infrared (NIR) region, and this limits their phototheranostic performance. Herein, we first exploit a diradicaloid molecular structure for enhancing NIR absorption to facilitate efficient photoacoustic imaging (PAI)-guided photothermal therapy (PTT). The donor-acceptor interaction in the diradicaloid molecule (DRM) leads to strong charge transfer resulting on obvious diradical characteristics, which is beneficial for NIR absorption. The DRM possesses excellent light-harvesting ability, with a mass extinction coefficient of ∼220 L g-1 cm-1, which is much higher than those (∼5-100 L g-1 cm-1) of typical organic molecules. After assembling into nanoparticles, they show good water dispersibility, good photostability, and impressive performance for PAI-guided PTT in vitro and in vivo. The impressive in vitro and in vivo performances show that developing small molecules with diradicaloid structures can be an effective approach for enhancing NIR harvesting capability for biomedical applications.


Assuntos
Neoplasias/diagnóstico por imagem , Neoplasias/terapia , Terapia Fototérmica , Espectroscopia de Luz Próxima ao Infravermelho/métodos , Nanomedicina Teranóstica/métodos , Células 3T3 , Células A549 , Animais , Materiais Biocompatíveis , Humanos , Camundongos , Nanopartículas
8.
J Cancer Res Clin Oncol ; 147(3): 703-712, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33386469

RESUMO

OBJECTIVE: The malignant transformation of normal bladder cells (SV-HUC-1) was induced by arsenite to explore the possible mechanism of circRNA-100284 influencing bladder cancer cell proliferation. METHODS: Normal bladder SV-HUC-1 cells were cultured with 2 µM arsenite to induce malignant transformation. After 0, 3, 6, 12, and 24 h of culture, the expression level of circRNA-100284 in cells was detected by quantitative real-time PCR. Western blotting assays were used to detect the expression levels of EZH2 and cyclin-D1 proteins in cells treated with different media. Cell cycle was analyzed by flow cytometry. In addition, through cell transfection and CCK-8 experiments, the effect and mechanism of circRNA-100284 targeting microRNA-217 on proliferation was determined. The interaction between HSP70 methylation and Aurora-B was determined by Western blotting and immunoprecipitation experiments. RESULTS: With prolonged contact time with arsenite, the expression level of circRNA-100284 in cells increased continuously (P < 0.05). Western blotting assays showed that the expression levels of EZH2 and cyclin-D1 proteins in arsenite-transformed cells increased. Flow cytometry and CCK-8 showed that circRNA-100284 accelerated cell cycle transition and cell proliferation through miR-217. Finally, after culturing human bladder cancer T24 cells, combined with immunoprecipitation and in vitro kinase experiments, it was found that K561- dimethyl HSP70 activated Aurora-B, thus promoting the proliferation of bladder cancer cells. CONCLUSION: CircRNA-100284 activates aurora kinase B by inducing methylation of HSP70 via microRNA-217 to promote the proliferation of bladder cancer cells.


Assuntos
Aurora Quinase B/metabolismo , Proteínas de Choque Térmico HSP70/metabolismo , MicroRNAs/metabolismo , RNA Circular/metabolismo , Neoplasias da Bexiga Urinária/metabolismo , Animais , Arsenitos/farmacologia , Aurora Quinase B/genética , Ciclo Celular/fisiologia , Linhagem Celular Tumoral , Proliferação de Células/fisiologia , Transformação Celular Neoplásica/induzido quimicamente , Ciclina D2/genética , Ciclina D2/metabolismo , Proteína Potenciadora do Homólogo 2 de Zeste/genética , Proteína Potenciadora do Homólogo 2 de Zeste/metabolismo , Ativação Enzimática , Feminino , Proteínas de Choque Térmico HSP70/genética , Xenoenxertos , Humanos , Metilação , Camundongos , MicroRNAs/genética , RNA Circular/genética , Regulação para Cima , Neoplasias da Bexiga Urinária/genética , Neoplasias da Bexiga Urinária/patologia
9.
Adv Ther ; 38(2): 1301-1313, 2021 02.
Artigo em Inglês | MEDLINE | ID: mdl-33432539

RESUMO

INTRODUCTION: We carried out this systemic review and meta-analysis of relevant randomized controlled trials to determine different dosage regimens of vardenafil in the treatment of male erectile dysfunction (ED). METHODS: Using appropriate keywords, we searched PubMed, the Cochrane Library, and Embase for relevant literature before March 2020. We evaluated odds ratio (OR), weighted mean difference (WMD), and 95% confidence interval (95% CI) to assess the results of each study. RESULTS: We included 14 studies with a total of 3221 patients. Compared with the placebo, vardenafil significantly increased International Erectile Function Index (IIEF) overall satisfaction (WMD 3.37, 95% CI 2.02-4.71), IIEF-erectile function (WMD 7.93, 95% CI 6.00-9.85), IIEF sexual desire (WMD 0.79, 95% CI 0.24-1.35), IIEF intercourse satisfaction (WMD 5.24, 95% CI 3.35-7.13), IIEF orgasmic function (WMD 3.81, 95% CI 2.26-5.35), Sexual Encounter Profile (SEP) Q2 (WMD 26.36, 95% CI 22.95-29.77), and SEP Q3 (WMD 35.18, 95% CI 31.89-38.48). CONCLUSIONS: Vardenafil demonstrated significant efficacy in the treatment of ED, but the optimal dose and course of vardenafil remain to be established.


Assuntos
Disfunção Erétil , Método Duplo-Cego , Disfunção Erétil/tratamento farmacológico , Humanos , Imidazóis/uso terapêutico , Masculino , Inibidores de Fosfodiesterase , Piperazinas , Sulfonas/uso terapêutico , Resultado do Tratamento , Triazinas/uso terapêutico , Dicloridrato de Vardenafila/uso terapêutico
10.
Am J Transl Res ; 12(9): 5730-5740, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33042452

RESUMO

In recent decades, growing data has suggested that microRNAs (miRNAs, miRs) play a critical role in the development of Sertoli cells (SC), including regulating SC maturation, synthesis, proliferation, and apoptosis. Previous reports of miRNA microarray have identified aberrant miR-4270 expression in patients with Sertoli-cell-only syndrome (SCOS). However, it is not known whether miR-4270 is associated with the pathogenesis of SCOS. In this study, we aimed to further investigate the roles and potential mechanisms of miR-4270 on SC proliferation and apoptosis. Our data confirmed that miR-4270 was significantly upregulated in SC of SCOS patients compared with healthy controls. EdU and CCK-8 assays showed silencing of miR-4270 by specific inhibitor significantly enhanced human SC and TM4 cells proliferation. ELISA and flow cytometry assays indicated that miR-4270 knockdown prominently suppressed the apoptosis of human SC and TM4 cells. Furthermore, expression of cell cycle genes, including CCNE1 (cyclin E1), CCND1 (cyclin D1) and CDK4 (cyclin dependent kinase 4), were obviously upregulated in human SC and TM4 cells by qRT-PCR assay after knockdown of miR-4270, while expression of cell apoptotic factors, including CASP3 (caspase 3), CASP6 (caspase 6) and CASP7 (caspase 7), were all markedly decreased. Notably, GADD45A (growth arrest and DNA damage inducible alpha) mRNA was downregulated in SC of SCOS patients, and negatively corrected with miR-4270 expression. Moreover, bioinformatics tools and dual-luciferase reporter assay identified that miR-4270 directly bound the 3'-UTR of GADD45A mRNA to inhibit GADD45A expression. Meanwhile, Western blots analysis validated that the protein expression levels of NOTCH1 (notch receptor 1) and HES1 (hes family bHLH transcription factor 1) were significantly increased in SC and TM4 cells after miR-4270 silencing or GADD45A overexpression. Taken together, our data demonstrated that miR-4270 regulates proliferation and apoptosis in SC of SCOS patients by inactivating NOTCH signaling pathway via GADD45A gene, which may offer a new insight into the development of human SC and provide a promising biomarker for the treatment of SCOS.

11.
Small ; 16(47): e2004551, 2020 11.
Artigo em Inglês | MEDLINE | ID: mdl-33125185

RESUMO

Water-splitting has been extensively studied especially for energy applications. It is often not paid with enough attention for biomedical applications. In fact, several innovative breakthroughs have been achieved in the past few years by employing water-splitting for treating cancer and other diseases. Interestingly, among these important works, only two reports have mentioned the term "water-splitting." For this reason, the importance of water-splitting for biomedical applications is significantly underestimated. This progress work is written with the aims to explain and summarize how the principle of water-splitting is employed to achieve therapeutic results not offered by conventional approaches. It is expected that this progress report will not only explain the importance of water-splitting to scientists in the biomedical fields, it should also draw attention from scientists working on energy applications of water-splitting.


Assuntos
Terapêutica , Água , Tecnologia Biomédica/métodos , Tecnologia Biomédica/tendências , Neoplasias/terapia , Terapêutica/métodos , Terapêutica/tendências , Água/química
12.
BMC Urol ; 20(1): 137, 2020 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-32873267

RESUMO

BACKGROUND: Inferior vena cava (IVC) aneurysms are extremely rare with variable clinical manifestations. Patients are usually asymptomatic or present with complications of thrombosis and rupture. To date, there have been only a few reports of the condition in the literature, and diagnosis of IVC aneurysms may be difficult. CASE PRESENTATION: A 33-year-old male patient presented to hospital because of a retroperitoneal mass found by computerized tomography during a health examination. He was asymptomatic, and post medical history and physical examination were unremarkable. Laboratory tests including tests for paraganglioma were all negative. Contrast-enhanced computed tomography scan revealed a stenosis of IVC in the suprarenal segment and two retroperitoneal mass on the right side of IVC. The larger one is about 3 cm in diameter and the smaller one is about 1 cm in diameter, which was considered as a retroperitoneal tumor with an enlarged lymph node. However, two IVC diverticular aneurysms were confirmed during the retroperitoneal laparoscopic exploration. The larger aneurysm was resected from the IVC successfully. Since the smaller aneurysm was about 1 cm in diameter without thrombosis, we did not resect it during surgery. The patient recovered well from surgery and discharged from our department successfully. CONCLUSIONS: This is the first report of multiple IVC aneurysms. Because of the extremely low prevalence of IVC diverticular aneurysm, it may be misdiagnosed as other disease. Due to the high rate of thrombosis, surgical treatment especially retroperitoneal laparoscopy is recommended for small diverticular aneurysms.


Assuntos
Aneurisma/diagnóstico , Neoplasias Retroperitoneais/diagnóstico , Veia Cava Inferior , Adulto , Aneurisma/patologia , Diagnóstico Diferencial , Humanos , Masculino
13.
Small ; 16(34): e2002672, 2020 08.
Artigo em Inglês | MEDLINE | ID: mdl-32697430

RESUMO

Multi-modality imaging-guided cancer therapy is considered as a powerful theranostic platform enabling simultaneous precise diagnosis and treatment of cancer. However, recently reported multifunctional systems with multiple components and sophisticate structures remain major obstacles for further clinical translation. In this work, a single-photomolecular theranostic nanoplatform is fabricated via a facile nanoprecipitation strategy. By encapsulating a semiconductor oligomer (IT-S) into an amphiphilic lipid, water-dispersible IT-S nanoparticles (IT-S NPs) are prepared. The obtained IT-S NPs have a very simple construction and possess ultra-stable near-infrared (NIR) fluorescence (FL)/photoacoustic (PA) dual-modal imaging and high photothermal conversion efficiency of 72.3%. Accurate spatiotemporal distribution profiles of IT-S NPs are successfully visualized by NIR FL/PA dual-modal imaging. With the comprehensive in vivo imaging information provided by IT-S NPs, tumor photothermal ablation is readily realized under precise manipulation of laser irradiation, which greatly improves the therapeutic efficacy without any obvious side effects. Therefore, the IT-S NPs allow high tumor therapeutic efficacy under the precise guidance of FL/PA imaging techniques and thus hold great potential as an effective theranostic platform for future clinical applications.


Assuntos
Hipertermia Induzida , Nanopartículas , Neoplasias , Técnicas Fotoacústicas , Linhagem Celular Tumoral , Humanos , Neoplasias/diagnóstico por imagem , Neoplasias/terapia , Imagem Óptica , Fototerapia , Nanomedicina Teranóstica
14.
Mikrochim Acta ; 187(5): 295, 2020 04 28.
Artigo em Inglês | MEDLINE | ID: mdl-32347383

RESUMO

A fluorescence method based on functionalized magnetic nanoparticles (FMNPs) and hybridization chain reaction (HCR) is developed for the enzyme-free amplified determination of thrombin. In the proposed design, aptamer against thrombin was hybridized with the capture DNA-modified magnetic nanoparticles to yield the FMNPs. In the presence of thrombin, aptamers are released due to the specific and high-affinity binding between thrombin and its aptamer. The exposed capture DNA subsequently hybridized with the partial sequence of helper DNA, and the vacant sequence of helper DNA further hybridized with HCR products which is pre-formed by the alternate hybridization of single-stranded DNAs (H1 and H2). The immobilized HCR products were then labeled with YOYO-1 for fluorescence measurement. Fluorescence signal intensity of labeled YOYO-1 was measured at an emission wavelength of 519 nm (excitation under 488 nm) and used for calibration. By taking advantage of HCR amplification, this direct assay strategy showed a linear response in the 20- to 200-pM concentration range, and the limit of detection is 9.2 pM which is about 3-orders of magnitude lower than the serum thrombin concentration (10 nM) that triggers blood clotting. This developed method can efficiently differentiate the target protein from a protein matrix, and it is verified by determination of thrombin in spiked serum samples with recoveries in the range of 94.5-103.3%. Graphical abstract A fluorometry method for thrombin detection using magnetic nanoparticles and enzyme-free hybridization chain reaction.


Assuntos
Aptâmeros de Nucleotídeos/química , DNA/química , Fluorometria , Nanopartículas de Magnetita/química , Hibridização de Ácido Nucleico , Trombina/análise , Humanos
15.
BMC Nephrol ; 21(1): 79, 2020 03 05.
Artigo em Inglês | MEDLINE | ID: mdl-32138685

RESUMO

BACKGROUND: Renal medullary carcinoma is a rare and aggressive tumor and often seen in young adults with sickle cell hemoglobinopathies. CASE PRESENTATION: We report a case of renal medullary carcinoma in a 29-year old male patient with an occupying renal lesion who presented with fever, flank pain and hematuria. The patient received intensive antibiotics treatment, but no improvement was seen. The symptoms disappeared after laparoscopic radical left nephrectomy. Postoperative pathological study showed that the mass was renal medullary carcinoma. CONCLUSIONS: Our case suggests that renal medullary carcinoma should be considered in differential diagnoses of patients with occupying renal lesions who have fever of unknown origin.


Assuntos
Infecções Bacterianas/diagnóstico , Carcinoma Medular/diagnóstico , Carcinoma de Células Renais/diagnóstico , Nefropatias/diagnóstico , Neoplasias Renais/diagnóstico , Adulto , Carcinoma Medular/cirurgia , Carcinoma de Células Renais/cirurgia , Diagnóstico Tardio , Diagnóstico Diferencial , Febre de Causa Desconhecida/etiologia , Hematúria/etiologia , Humanos , Neoplasias Renais/cirurgia , Masculino , Nefrectomia , Dor/etiologia , Perda de Peso
16.
J Invest Surg ; 33(3): 203-210, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30461324

RESUMO

BACKGROUND: Incidence of intraoperative vaginal perforation is generally considered to be low but varies among different procedures. Vaginal perforation could not only prolong the surgeries and aggravate surgical trauma but also result in postoperative discomfort or even a second surgery. METHOD: Vaginal perforation, vaginal epithelial perforation, vaginal wall perforation, vaginal penetration, urinary incontinence were searched in PubMed, Cochrane, Embase database to identify the qualified clinical trial and relevant literature sources were also searched. RESULTS: A total of 9223 cases of from 33 trials from literatures and 387 cases from our own trail were analyzed, which provided detailed data on intraoperative vaginal perforation. Incidence of intraoperative vaginal perforation during mid-urethral sling surgery treating stress urinary incontinence was generally low, which was 1.56%. Incidence of intraoperative vaginal perforation during transobturator (TOR) procedure was higher than that during retropubic (RPR) procedure, which were 2.11% and 0.89% respectively. Incidence of intraoperative vaginal perforation during outside-to-inside TOR procedure like TOT and MONARC was higher than that during inside-to-outside TOR procedure like TVT-O, which were 2.74% and 1.52%, respectively. Incidence of intraoperative vaginal perforation during single-incision surgery like H-type TVT-SECUR reached 1.97%, while no report on U-type TVT-SECUR surgeries. CONCLUSION: The incidence of intraoperative vaginal during mid-urethral sling procedures for female stress urinary incontinence is fairly high. Vaginal perforation was more common in trans-obturator route (TOR) than retropubic route (RPR). In TOR route, it was less frequent in inside-to-outside procedure than outside-to-inside procedure. Surgery proficiency could also have an impact on this complication.


Assuntos
Complicações Intraoperatórias/epidemiologia , Slings Suburetrais/efeitos adversos , Incontinência Urinária por Estresse/cirurgia , Procedimentos Cirúrgicos Urológicos/efeitos adversos , Vagina/lesões , Feminino , Humanos , Incidência , Complicações Intraoperatórias/etiologia , Procedimentos Cirúrgicos Urológicos/instrumentação , Procedimentos Cirúrgicos Urológicos/métodos
17.
Angew Chem Int Ed Engl ; 59(2): 632-636, 2020 01 07.
Artigo em Inglês | MEDLINE | ID: mdl-31670869

RESUMO

Traditional photosensitizers (PSs) show reduced singlet oxygen (1 O2 ) production and quenched fluorescence upon aggregation in aqueous media, which greatly affect their efficiency in photodynamic therapy (PDT). Meanwhile, non-targeting PSs generally yield low efficiency in antibacterial performance due to their short lifetimes and small effective working radii. Herein, a water-dispersible membrane anchor (TBD-anchor) PS with aggregation-induced emission is designed and synthesized to generate 1 O2 on the bacterial membrane. TBD-anchor showed efficient antibacterial performance towards both Gram-negative (Escherichia coli) and Gram-positive bacteria (Staphylococcus aureus). Over 99.8 % killing efficiency was obtained for methicillin-resistant S. aureus (MRSA) when they were exposed to 0.8 µm of TBD-anchor at a low white light dose (25 mW cm-2 ) for 10 minutes. TBD-anchor thus shows great promise as an effective antimicrobial agent to combat the menace of multidrug-resistant bacteria.


Assuntos
Farmacorresistência Bacteriana Múltipla/efeitos dos fármacos , Testes de Sensibilidade Microbiana/métodos , Fármacos Fotossensibilizantes/uso terapêutico , Humanos , Fármacos Fotossensibilizantes/farmacologia
18.
ACS Nano ; 13(11): 12901-12911, 2019 11 26.
Artigo em Inglês | MEDLINE | ID: mdl-31682416

RESUMO

We developed a biodegradable photothermal therapeutic (PTT) agent, π-conjugated oligomer nanoparticles (F8-PEG NPs), for highly efficient cancer theranostics. By exploiting an oligomer with excellent near-infrared (NIR) absorption, the nanoparticles show a high photothermal conversion efficiency (PCE) up to 82%, surpassing those of reported inorganic and organic PTT agents. In addition, the oligomer nanoparticles show excellent photostability and good biodegradability. The F8-PEG NPs are also demonstrated to have excellent biosafety and PTT efficacy both in vitro and in vivo. This contribution not only proposes a promising oligomer-based PTT agent but also provides insight into developing highly efficient nanomaterials for cancer theranostics.


Assuntos
Nanopartículas/química , Neoplasias/terapia , Fototerapia , Nanomedicina Teranóstica , Células A549 , Animais , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Células HeLa , Humanos , Camundongos , Nanopartículas/metabolismo , Neoplasias/patologia
19.
J BUON ; 24(3): 1210-1218, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31424681

RESUMO

PURPOSE: To investigate the correlations of the expression of micro ribonucleic acid (miR)-203 with the onset and prognosis of bladder cancer. METHODS: Human bladder cancer T24 cell line with miR-203 overexpression were constructed. Effects of miR-203 expression on cell proliferation and apoptosis were detected via methyl thiazolyl tetrazolium (MTT) assay and flow cytometry, respectively. Fifty-eight patients with bladder cancer treated in our hospital were selected, and para-carcinoma tissues were used as controls. The expression of miR-203 in bladder cancer tissues was detected via quantitative polymerase chain reaction (qPCR), and the survival time of patients with bladder cancer was recorded in detail. Moreover, correlations of miR-203 expression with onset and prognosis of patients were evaluated via multivariate Cox regression analysis. RESULTS: Results of qPCR revealed that after T24 cells were transfected with miR-203, the miR-203 expression level in the overexpression group was significantly higher than that in the control group (p<0.01), the cell proliferation capacity was significantly lower than that in the control group (p<0.01), and the apoptotic level was also significantly higher than that in the control group (p<0.01). The miR-203 expression level in carcinoma tissues of patients with bladder cancer was obviously lower than that in the para-carcinoma tissues (p<0.01). The miR-203 expression had close correlations with the tumor size, differentiation grade and tumor stage (p<0.01), and the 5-year survival rate of patients in low miR-203 expression group was remarkably lower than that in the high miR-203 expression group (p<0.01). Besides, the results of multivariate Cox regression analysis revealed that the tumor grade of differentiation, tumor-node-metastasis (TNM) stage and miR-203 expression were closely related to the prognosis of patients (p<0.01). CONCLUSION: The high expression of miR-203 can significantly reduce the proliferation and promote the apoptosis of bladder cancer cells. The increased expression level of miR-203 can effectively increase the survival rate and improve the prognosis of patients.


Assuntos
MicroRNAs/metabolismo , Neoplasias da Bexiga Urinária/genética , Linhagem Celular Tumoral , Feminino , Humanos , Masculino , Prognóstico , Taxa de Sobrevida , Transfecção , Neoplasias da Bexiga Urinária/mortalidade , Neoplasias da Bexiga Urinária/patologia
20.
Chem Sci ; 11(3): 888-895, 2019 Dec 11.
Artigo em Inglês | MEDLINE | ID: mdl-34123067

RESUMO

Rational manipulation of energy utilization from excited-state radiation of theranostic agents with a donor-acceptor structure is relatively unexplored. Herein, we present an effective strategy to tune the exciton dynamics of radiative excited state decay for augmenting two-photon nanotheranostics. As a proof of concept, two thermally activated delayed fluorescence (TADF) molecules with different electron-donating segments are engineered, which possess donor-acceptor structures and strong emissions in the deep-red region with aggregation-induced emission characteristics. Molecular simulations demonstrate that change of the electron-donating sections could effectively regulate the singlet-triplet energy gap and oscillator strength, which promises efficient energy flow. A two-photon laser with great permeability is used to excite TADF NPs to perform as theranostic agents with singlet oxygen generation and fluorescence imaging. These unique performances enable the proposed TADF emitters to exhibit tailored balances between two-photon singlet oxygen generation and fluorescence emission. This result demonstrates that TADF emitters can be rationally designed as superior candidates for nanotheranostic agents by the custom controlling exciton dynamics.

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