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1.
Plant Biotechnol J ; 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31368610

RESUMO

China is the origin and evolutionary centre of Oriental pears. Pyrus betuleafolia is a wild species native to China and distributed in the northern region, and it is widely used as rootstock. Here, we report the de novo assembly of the genome of P. betuleafolia-Shanxi Duli using an integrated strategy that combines PacBio sequencing, BioNano mapping and chromosome conformation capture (Hi-C) sequencing. The genome assembly size was 532.7 Mb, with a contig N50 of 1.57 Mb. A total of 59 552 protein-coding genes and 247.4 Mb of repetitive sequences were annotated for this genome. The expansion genes in P. betuleafolia were significantly enriched in secondary metabolism, which may account for the organism's considerable environmental adaptability. An alignment analysis of orthologous genes showed that fruit size, sugar metabolism and transport, and photosynthetic efficiency were positively selected in Oriental pear during domestication. A total of 573 nucleotide-binding site (NBS)-type resistance gene analogues (RGAs) were identified in the P. betuleafolia genome, 150 of which are TIR-NBS-LRR (TNL)-type genes, which represented the greatest number of TNL-type genes among the published Rosaceae genomes and explained the strong disease resistance of this wild species. The study of flavour metabolism-related genes showed that the anthocyanidin reductase (ANR) metabolic pathway affected the astringency of pear fruit and that sorbitol transporter (SOT) transmembrane transport may be the main factor affecting the accumulation of soluble organic matter. This high-quality P. betuleafolia genome provides a valuable resource for the utilization of wild pear in fundamental pear studies and breeding.

2.
Gene ; 714: 143992, 2019 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-31330234

RESUMO

Increasing studies have demonstrated the important roles of circular RNAs (circRNAs) in human malignancies. Nevertheless, the molecular mechanisms and functions of circRNAs in hepatocellular carcinoma (HCC) are still not fully understood. In the present study, we evaluated circ_0021093 expression in 82 pairs of HCC tissues and 5 cell lines by qRT-PCR. The clinical implications of circ_0021093 were evaluated. In addition, the viability, apoptosis, migration and invasion capacities of different HCC cells were evaluated by gain-/loss-of-function experiments. Target prediction and dual-luciferase reporter experiments were performed to identify the molecular mechanisms of circ_0021093. Upregulation of circ_0021093 was found in HCC tumor samples and cells. Additionally, upregulated circ_0021093 was related to adverse clinical characteristics and an unfavorable prognosis. Furthermore, downregulated circ_0021093 attenuated cell growth, migration and invasion but increased cell apoptosis. By contrast, ectopically expressed circ_0021093 enhanced the abovementioned malignant biological behaviors. For mechanism exploration, circ_0021093 sponges of miR-766-3p were used in HCC cells. In addition, we found that metastasis-associated protein 3 (MTA3) was a direct target of miR-766-3p and that the oncogenic function of circ_0021093 was partly dependent on the miR-766-3p/MTA3 axis according to rescue assays. In conclusion, the circ_0021093/miR-766-3p/MTA3 regulatory axis may be an effective therapeutic target for HCC.


Assuntos
Carcinoma Hepatocelular/genética , Neoplasias Hepáticas/genética , MicroRNAs/genética , Proteínas de Neoplasias/genética , RNA/genética , Apoptose/genética , Carcinoma Hepatocelular/patologia , Linhagem Celular Tumoral , Movimento Celular/genética , Proliferação de Células/genética , Progressão da Doença , Regulação para Baixo/genética , Feminino , Regulação Neoplásica da Expressão Gênica/genética , Células Hep G2 , Humanos , Neoplasias Hepáticas/patologia , Masculino , Pessoa de Meia-Idade , Prognóstico , Regulação para Cima/genética
3.
Int J Genomics ; 2018: 3505428, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30510960

RESUMO

We screened bacteria that use E2 as its sole source of carbon and energy for growth and identified them as Rhodococcus, and we named them DSSKP-R-001. For a better understanding of the metabolic potential of the strain, whole genome sequencing of Rhodococcus DSSKP-R-001 and annotation of the functional genes were performed. The genomic sketches included a predicted protein-coding gene of approximately 5.4 Mbp with G + C content of 68.72% and 5180. The genome of Rhodococcus strain DSSKP-R-001 consists of three replicons: one chromosome and two plasmids of 5.2, 0.09, and 0.09, respectively. The results showed that there were ten steroid-degrading enzymes distributed in the whole genome of the strain. The existence and expression of estradiol-degrading enzymes were verified by PCR and RTPCR. Finally, comparative genomics was used to compare multiple strains of Rhodococcus. It was found that Rhodococcus DSSKP-R-001 had the highest similarity to Rhodococcus sp. P14 and there were 2070 core genes shared with Rhodococcus sp. P14, Rhodococcus jostii RHA1, Rhodococcus opacus B4, and Rhodococcus equi 103S, showing evolutionary homology. In summary, this study provides a comprehensive understanding of the role of Rhodococcus DSSKP-R-001 in estradiol-efficient degradation of these assays for Rhodococcus. DSSKP-R-001 in bioremediation and evolution within Rhodococcus has important meaning.

4.
J Sep Sci ; 41(21): 4001-4013, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30194817

RESUMO

There are strong economic drivers for understanding the process of fruit post-harvest softening. In this study, liquid chromatography with electrospray ionization tandem mass spectrometry was used to analyze metabolite changes in 'Zaoshu Shanli' fruit after different storage periods; this wild accession of Pyrus ussuriensis presents good fruit quality and relatively low flesh firmness after fruit storage. The lipid metabolites in 'Zaoshu Shanli' fruit were significantly higher at 18 days of storage compared with those at 0 days of storage, and glycerophospholipid metabolism was different metabolic pathway. It was therefore speculated that lipid metabolism play an important role in pear fruit post-harvest processes and softening. Furthermore, the abscisic acid and trans-zeatin contents in 'Zaoshu Shanli' fruit at 18 days of storage were significantly greater than those at 0 days of storage. Therefore, it was speculated that the abovementioned hormones play an important role in pear fruit post-harvest softening. Together, these results provide fundamental insight into the reasonable control of pear fruit post-harvest softening and lay a solid foundation for additional research.

5.
Curr Neuropharmacol ; 16(1): 29-36, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-28486926

RESUMO

BACKGROUND: Epilepsy is a syndrome of brain dysfunction caused by spontaneous, abnormal discharge. Many anti-epileptic drugs have developed in past decades. 5-HT is an important neurotransmitter in the central and peripheral nervous system of the human body which is involved in a number of physiological activities, such as sensation, movement, and behavior. 5-HT subtype have been divided into seven sub-groups from 5-HT1 to 5HT7. However, the role of 5-HT3 receptor on epilepsy is unclear. Therefore, in this article, the possible role of 5-HT3 receptor on epilepsy was systemically reviewed. METHODS: Data were collected from Web of Science, Medline, Pubmed, Scopus, through searching of these keywords: "5-HT3" and "epilepsy". RESULTS: An increasing number of studies have shown that the activation of the 5-HT3 receptor can inhibit epileptic seizures, while inhibition of the 5-HT3 receptor can promote spike waves. CONCLUSION: In this review, we discuss the relationship between the 5HT3 receptor and epilepsy; this review may provide a new insight for clinical application of epilepsy treatment.


Assuntos
Epilepsia/metabolismo , Receptores 5-HT3 de Serotonina/metabolismo , Serotoninérgicos/uso terapêutico , Animais , Encéfalo/efeitos dos fármacos , Encéfalo/metabolismo , Bases de Dados Factuais/estatística & dados numéricos , Epilepsia/tratamento farmacológico , Epilepsia/genética , Humanos , Receptores 5-HT3 de Serotonina/genética , Serotoninérgicos/farmacologia
6.
Exp Ther Med ; 14(2): 1241-1247, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28781621

RESUMO

Skeletal muscle atrophy is often caused by catabolic conditions including fasting, disuse, aging and chronic diseases, such as chronic obstructive pulmonary disease. Atrophy occurs when the protein degradation rate exceeds the rate of protein synthesis. Therefore, maintaining a balance between the synthesis and degradation of protein in muscle cells is a major way to prevent skeletal muscle atrophy. Ginsenoside Rg1 (Rg1) is a primary active ingredient in Panax ginseng, which is considered to be one of the most valuable herbs in traditional Chinese medicine. In the current study, Rg1 was observed to inhibit the expression of MuRF-1 and atrogin-1 in C2C12 muscle cells in a starvation model. Rg1 also activated the phosphorylation of mammalian target of rapamycin (mTOR), protein kinase B (AKT), and forkhead transcription factor O, subtypes 1 and 3a. This phosphorylation was inhibited by LY294002, a phosphatidylinositol 3-kinase inhibitor. These data suggest that Rg1 may participate in the regulation of the balance between protein synthesis and degradation, and that the function of Rg1 is associated with the AKT/mTOR/FoxO signaling pathway.

7.
Oncol Lett ; 13(2): 599-604, 2017 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-28356935

RESUMO

Tumor invasion and metastasis are the critical steps in determining the aggressive phenotype of human cancers. Melittin, a major component of bee venom, has been reported to induce apoptosis in several cancer cells. However, the mechanisms of melittin involvement in cancer invasion and metastasis remain unclear. Our previous study indicated that melittin inhibits cyclophilin A (CypA), a ubiquitously distributed peptidylprolyl cis-trans isomerase, in macrophage cells. In the present study, the Transwell assay results showed that melittin may downregulate the invasion level of MCF-7 cells in a dose-dependent manner. Additionally, it was also found, using flow cytometry and reverse transcription-polymerase chain reaction, that melittin decreased the expression of cluster of differentiation (CD)147 and matrix metallopeptidase-9 (MMP-9), whereas CypA upregulated the expression of CD147 and MMP-9. Overall, the present study indicated that melittin decreased the invasion level of MCF-7 cells by downregulating CD147 and MMP-9 by inhibiting CypA expression. The results of the present study provide an evidence for melittin in anticancer therapy and mechanisms.

8.
Biodegradation ; 28(2-3): 145-157, 2017 06.
Artigo em Inglês | MEDLINE | ID: mdl-28168378

RESUMO

A novel micro-pressure swirl reactor (MPSR) was designed and applied to treat domestic wastewater at low temperature by acclimating microbial biomass with steadily decreasing temperature from 15 to 3 °C. Chemical oxygen demand (COD) was constantly removed by 85% and maintained below 50 mg L-1 in the effluent during the process. When the air flow was controlled at 0.2 m3 h-1, a swirl circulation was formed in the reactor, which created a dissolved oxygen (DO) gradient with a low DO zone in the center and a high DO zone in the periphery for denitrification and nitrification. 81% of total nitrogen was removed by this reactor, in which ammonium was reduced by over 90%. However, denitrification was less effective because of the presence of low levels of oxygen. The progressively decreasing temperature favored acclimation of psychrophilic bacteria in the reactor, which replaced mesophilic bacteria in the process of treatment.


Assuntos
Análise da Demanda Biológica de Oxigênio , Reatores Biológicos/microbiologia , Nitrogênio/isolamento & purificação , Pressão , Temperatura Ambiente , Águas Residuárias/química , Amônia/análise , Bactérias/metabolismo , Biodegradação Ambiental , Biodiversidade , Eletroforese em Gel de Gradiente Desnaturante , Nitratos/análise , Nitritos/análise , Oxigênio/análise , Fatores de Tempo , Eliminação de Resíduos Líquidos
9.
PLoS One ; 10(12): e0143819, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26623652

RESUMO

The magnetic graphene oxide (MGO) was successfully synthesised by the in situ chemical co-precipitation method with Fe3+, Fe2+ and graphene oxide (GO) in laboratory and, was used as an adsorbent for disinfection by-product (DBP) precursors removing from four natural surface water samples. The results indicate that various DBPs formation significantly decreased by 7-19% to 78-98% for the four samples after MGO treatment and, the treatment process was rapidly reached equilibrium within 20 minutes. The DBP precursors removal efficiency decreased with the increasing pH value from 4 to 10. Hydrophobic compounds (humic acid and fulvic acid) are more sensitive to MGO, whereas hydrophilic and nitrogenous compounds (aromatic proteins) are more insensitive. MGO could be regenerated by using 20% (v/v) ethanol and, the DBP precursors removal efficiency can stay stable after five cycles. These results indicate that MGO can be utilized as a promising adsorbent for the removal of DBP precursors from natural surface water.


Assuntos
Desinfetantes , Grafite/química , Compostos de Ferro/química , Fenômenos Magnéticos , Óxidos/química , Poluentes Químicos da Água , Purificação da Água/métodos , Substâncias Húmicas , Concentração de Íons de Hidrogênio , Compostos de Nitrogênio
10.
PLoS One ; 10(12): e0142700, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26630014

RESUMO

The renewable pine needles was used as an adsorbent to remove phosphorus from aqueous solutions. Using batch experiments, pine needles pretreated with alkali-isopropanol (AI) failed to effectively remove phosphorus, while pine needles modified with lanthanum hydroxide (LH) showed relatively high removal efficiency. LH pine needles were effective at a wide pH ranges, with the highest removal efficiency reaching approximately 85% at a pH of 3. The removal efficiency was kept above 65% using 10 mg/L phosphorus solutions at desired pH values. There was no apparent significant competitive behavior between co-existing anions of sulfate, nitrate, and chloride (SO4(2-), NO3(-) and Cl(-)); however, CO3(2-) exhibited increased interfering behavior as concentrations increased. An intraparticle diffusion model showed that the adsorption process occurred in three phases, suggesting that a boundary layer adsorption phenomena slightly affected the adsorption process, and that intraparticle diffusion was dominant. The adsorption process was thermodynamically unfavorable and non-spontaneous; temperature increases improved phosphorus removal. Total organic carbon (TOC) assays indicated that chemical modification reduced the release of soluble organic compounds from 135.6 mg/L to 7.76 mg/L. This new information about adsorption performances provides valuable information, and can inform future technological applications designed to remove phosphorus from aqueous solutions.


Assuntos
Lantânio/química , Fósforo/isolamento & purificação , Pinus/química , Poluentes Químicos da Água/química , Purificação da Água/métodos , Adsorção , Concentração de Íons de Hidrogênio , Cinética , Temperatura Ambiente , Termodinâmica
11.
Int J Mol Med ; 36(3): 733-8, 2015 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-26135903

RESUMO

Apelin-13 is extensively expressed in various tissues, particularly breast tissue. Apelin­13 has been shown to promote tumor proliferation in various types of cancer, including hepatocellular, lung and ovarian cancer. However, the effect and molecular mechanism of apelin­13 in breast cancer cells remains unclear. The present study investigated the effect of apelin­13 on MCF­7. Therefore, cell proliferation was determined by MTT and flow cytometry analysis. The results revealed that apelin­13 markedly increased cell proliferation. Transwell assays demonstrated that apelin­13 increased MCF­7 cell invasion. Apelin­13 also markedly increased the expression of cyclin D1, extracellular matrix metalloproteinase­1 and amplified in breast cancer 1 (AIB1) in a dose­dependent manner by polymerase chain reaction assays. To study the molecular mechanism, cell proliferation, invasion and cyclin D1 were inhibited by pre­treatment with 10 µM of PD98059 (ERK(1/2) inhibitor). Western blotting results suggested that apelin­13 significantly enhances the expression of p­ERK(1/2) in a concentration­dependent manner. In conclusion, the results suggest that apelin­13 promoted MCF-7 cell proliferation and invasion via the ERK1/2/AIB1 signaling pathway.


Assuntos
Neoplasias da Mama/metabolismo , Neoplasias da Mama/patologia , Mama/patologia , Peptídeos e Proteínas de Sinalização Intercelular/metabolismo , Sistema de Sinalização das MAP Quinases , Mama/metabolismo , Neoplasias da Mama/genética , Proliferação de Células , Ciclina D1/genética , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Células MCF-7 , Metaloproteinase 1 da Matriz/genética , Proteína Quinase 1 Ativada por Mitógeno/metabolismo , Proteína Quinase 3 Ativada por Mitógeno/metabolismo , Invasividade Neoplásica/patologia , Coativador 3 de Receptor Nuclear/genética , Fosforilação
12.
J Hazard Mater ; 292: 173-9, 2015 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-25804792

RESUMO

Iron sludge, produced from filtration and backwash of groundwater treatment plant, has long been considered as a waste for landfill. In this study, iron sludge was reused to synthesize Fe3O4 magnetic particles (MPs) by using a novel solvothermal process. Iron sludge contained abundant amounts of silicon, iron, and aluminum and did not exhibit magnetic properties. After treatment for 4h, the amorphous Fe in iron sludge was transformed into magnetite Fe3O4, which could be easily separated from aqueous solution with a magnet. The prepared particles demonstrated the intrinsic properties of soft magnetic materials and could aggregate into a size of 1 µm. MPs treated for 10h exhibited excellent magnetic properties and a saturation magnetization value of 9 emu/g. The obtained particles presented the optimal adsorption of methylene blue under mild conditions, and the maximum adsorption capacity was 99.4 mg/g, which was higher than that of granular active carbon. The simple solvothermal method can be used to prepare Fe3O4 MPs from iron sludge, and the products could be applied to treatment of dyeing wastewater.


Assuntos
Água Subterrânea , Magnetismo , Azul de Metileno/química , Esgotos , Adsorção , Microscopia Eletrônica de Varredura , Nitrogênio/química , Espectroscopia Fotoeletrônica , Espectroscopia de Mossbauer , Difração de Raios X
13.
Toxicol Lett ; 232(1): 1-9, 2015 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-25280774

RESUMO

Oxidative stress-induced DNA damage results in over-activation of poly(ADP-ribose) polymerase 1 (PARP1), leading to parthanatos, a newly discovered cell elimination pathway. Inhibition of PARP1-dependent cell death has shown to improve the outcome of diseases, including stroke, heart ischemia, and neurodegenerative diseases. In the present study we aimed to detect whether estrogen plays a protective role in inhibiting parthanatos. We utilized human mammary adenocarcinoma cells (MCF7) that abundantly express the estrogen receptor alpha and beta (ERα and ERß). Parthanatos was induced by challenging the cells with hydrogen peroxide (H2O2). Microscopic imaging and molecular biological techniques, such as Western blot analysis and RNA interference, were performed. The results showed 17ß estradiol (E2) protected MCF7 cells from PARP1-dependent cell death by decreasing protein PARylation, and AIF translocation into nuclei/nucleoli. Down-regulation of ERα expression by siRNA before E2 addition resulted in the failure of the E2-mediated inhibition of H2O2-induced protein PARylation and AIF nucleolar translocation. Together these data suggest that estrogen via its alpha-type receptor inhibits oxidative stress-induced, PARP1-dependent cell death. The present study provided us insight into how to apply hormone therapy in intervention of parthanatos-implicated ischemic and degenerative diseases.


Assuntos
Antioxidantes/farmacologia , Fator de Indução de Apoptose/metabolismo , Apoptose/efeitos dos fármacos , Neoplasias da Mama/metabolismo , Nucléolo Celular/efeitos dos fármacos , Estradiol/farmacologia , Receptor alfa de Estrogênio/agonistas , Estresse Oxidativo/efeitos dos fármacos , Poli(ADP-Ribose) Polimerase-1/metabolismo , Transporte Ativo do Núcleo Celular , Neoplasias da Mama/genética , Neoplasias da Mama/patologia , Nucléolo Celular/metabolismo , Nucléolo Celular/patologia , Citoproteção , Fragmentação do DNA , Relação Dose-Resposta a Droga , Ativação Enzimática , Receptor alfa de Estrogênio/genética , Receptor alfa de Estrogênio/metabolismo , Feminino , Humanos , Peróxido de Hidrogênio/toxicidade , Células MCF-7 , Interferência de RNA , Transdução de Sinais/efeitos dos fármacos , Fatores de Tempo , Transfecção
14.
J Biochem Mol Toxicol ; 26(7): 264-9, 2012 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-22730333

RESUMO

The transcription factor-E2-related factor 2 (Nrf2) is an important regulator against the process of oxidative stress. It can effectively scavenge oxygen-free radicals within cells to maintain homeostasis. In this study, we cultured primary myocardial cells, established the hypoxia/reoxygenation (H/R) model to simulate myocardial ischemia/reperfusion injury, and examined effects of 17ß-estradiol (E2) on the quantitative changes of Nrf2 in cytosolic and nuclear extracts, the mRNA expression of heme oxygenase 1 (HO-1), superoxide dismutase (Cu/Zn-SOD), glutathione S transferase (GST), and glutamate cysteine ligase amide (GCL) of each model group by Western blot assays and reverse transcription polymerase chain reaction, to investigate the effects of E2 against H/R/ injury in cultured myocardial cells. The present study shows that E2 can upregulate Nrf2 in nuclear extracts and increase the expression of HO-1, Cu/Zn-SOD, GST, and GCL significantly during H/R injury. Hence, our present findings suggest that E2 exhibits its antioxidant role by upregulating Nrf2 in nuclear extracts.


Assuntos
Antioxidantes/metabolismo , Estradiol/farmacologia , Miócitos Cardíacos/enzimologia , Fator 2 Relacionado a NF-E2/metabolismo , Animais , Núcleo Celular/enzimologia , Células Cultivadas , Citosol/enzimologia , Glutamato-Cisteína Ligase/metabolismo , Glutationa Transferase/metabolismo , Heme Oxigenase-1/metabolismo , Fator 2 Relacionado a NF-E2/genética , Estresse Oxidativo , Transporte Proteico/efeitos dos fármacos , Ratos , Ratos Sprague-Dawley , Traumatismo por Reperfusão/induzido quimicamente , Superóxido Dismutase/metabolismo , Regulação para Cima/efeitos dos fármacos
16.
Sheng Li Ke Xue Jin Zhan ; 38(2): 120-4, 2007 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-17633224

RESUMO

Twenty years ago, some foreign-investigators reported that omega-hydroxylation of arachidonic acid (AA) was catalyzed by cytochrome P-450 enzymes in some tissue to 20-hydroxyeicosatetraenoic acid (20-HETE). As a second messenger, it plays a pivotal role in the regulation of vascular tone, renal function, cerebral blood flow and lung vasodilator. Exogenous administeration of 20-HETE also plays an important role in the regulation of the coronary circulation. Moreover, hypertension, toxemia of pregnancy and other diseases is related to the formation of the 20-HETE. However, there is few domestic report about 20-HETE. Therefore, the domestic investigators should be given attention to 20-HETE adequately in the future. In this paper, roles of 20-HETE on physiologic and pathologic regulation in organism were reviewed.


Assuntos
Ácidos Hidroxieicosatetraenoicos/fisiologia , Músculo Liso Vascular/fisiologia , Sistema Vasomotor/fisiologia , Animais , Feminino , Humanos , Hipertensão/fisiopatologia , Pré-Eclâmpsia/fisiopatologia , Gravidez
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