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1.
Nat Prod Res ; : 1-6, 2021 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-34525866

RESUMO

A new unsaturated fatty acid trewioidesine A (1), together with seven known compounds (2 - 8) were isolated from the rhizomes of Alchornea trewioides (Benth.) Muell. Arg. Their structures were established on the basis of extensive spectroscopic data interpretation (1 D and 2 D NMR, and HRESIMS). The absolute configuration of 1 was determined by electronic circular dichroism (ECD) calculations, confirming as trewioidesine A. The functionality of isolated compounds was tested in cultured PC12 cells, a cell line from rat pheochromocytoma. Trewioidesine A was the one showing robust activity in inducing neuronal differentiation: the induction was synergized when co-applied with nerve growth factor (NGF). In addition, a neurofilament 200 (NF200) promoter-luciferase (pNF200-Luc) reporter was used to evaluate the differentiating ability in the transfected PC12 cells for the isolated compounds. Trewioidesine A exhibited a strong NF200 promoter activation, and application of trewioidesine A with low dose of NGF significantly induced the promoter activity over 50%.

2.
Fitoterapia ; 154: 105029, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34506872

RESUMO

Four new chalchonoid trimers, named cochinchinenins N-Q (1-4), along with a pair of known enantiomers (5-6), were isolated from the total phenolic extract of Chinese dragon's blood (the red resin of Dracaena cochinchinensis). The planar structures of 1-4 were elucidated by extensive spectroscopic analysis including HRESIMS and 1D/2D NMR. The absolute configurations of new compounds were established by ECD data. Compound 1 exhibited significant inhibition of nitric oxide production in lipopolysaccharide-stimulated BV-2 microglial cells with IC50 value of 11.5 ± 1.7 µM.


Assuntos
Chalconas/farmacologia , Dracaena/química , Microglia/efeitos dos fármacos , Extratos Vegetais/química , Animais , Linhagem Celular , Chalconas/isolamento & purificação , Medicamentos de Ervas Chinesas/farmacologia , Camundongos , Óxido Nítrico , Compostos Fitoquímicos/isolamento & purificação , Compostos Fitoquímicos/farmacologia , Resinas Vegetais/química
3.
Zhongguo Zhong Yao Za Zhi ; 46(16): 4131-4138, 2021 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-34467724

RESUMO

Eleven condensed tannins were isolated from the roots of Indigofera stachyodes by various column chromatography techniques including silica gel, octadecyl silica(ODS), Sephadex LH-20, and semi-preparative high performance liquid chromatography(HPLC). These compounds were identified on the basis of physicochemical properties, nuclear magnetic resonance(NMR) and mass spectrometry(MS) data as stachyotannin A(1), epicatechin-(2ß→O→7,4ß→8)-epiafzelechin-(4ß→8)-catechin(2), cinnamtannin D1(3), cinnamtannin B1(4), epicatechin-(2ß→O→7,4ß→8)-epiafzelechin-(4α→8)-epicatechin(5), gambiriin C(6), proanthocyanidin A1(7), proanthocyanidin A2(8), aesculitannin B(9), proanthocyanidin A4(10), and procyanidin B5(11). Compound 1 is a new compound. Compounds 2-11 were isolated from Indigofera for the first time. Furthermore, compounds 1, 2, and 4-11 showed inhibitory effects on thrombin-induced ATP release in platelets.


Assuntos
Indigofera , Proantocianidinas , Cromatografia Líquida de Alta Pressão , Espectroscopia de Ressonância Magnética , Extratos Vegetais
4.
Zhongguo Zhong Yao Za Zhi ; 46(18): 4841-4848, 2021 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-34581096

RESUMO

Longxue Tongluo Capsules(LTC) has good efficacy against blood stasis syndrome during the recovery period of ischemic stroke. Its main active ingredient is the phenolic extract of Chinese dragon's blood. In our previous study, the primary mass fragmentation pathways of phenolic derivatives from LTC were clarified. Herein, the metabolites in rat plasma were characterized following the oral administration of loureirin A and loureirin C using liquid chromatography coupled with hybrid ion trap/time-of-flight mass spectro-metry(LC-IT-TOF-MS), with 18 and 55 metabolites identified, respectively. On this basis, with the help of the obtained accurate molecular weight, characteristic fragment ions, reference comparison, combined with LTC database and natural products database self-created in our group, 18 prototypes and 106 metabolites were tentatively identified in rat plasma after oral gavage of LTC at a dose of 500 mg·kg~(-1). Glucuronidation, sulfonation, and methylation were major biotransformation pathways of LTC. This study preliminarily clarified the LTC constituents absorbed into blood and laid the foundation for clarifying the effective substances of LTC.


Assuntos
Medicamentos de Ervas Chinesas , Administração Oral , Animais , Cápsulas , Cromatografia Líquida de Alta Pressão , Cromatografia Líquida , Cromatografia Gasosa-Espectrometria de Massas , Ratos
5.
Zhongguo Zhong Yao Za Zhi ; 46(9): 2215-2219, 2021 May.
Artigo em Chinês | MEDLINE | ID: mdl-34047123

RESUMO

This study aims to study the chemical components from the gum resin of Boswellia carterii. Five cembranoid diterpenes were isolated from the gum resin of B. carterii by various of column chromatographies including silica gel, Sephadex LH-20, and semi-preparative HPLC. Their structures were identified on the basis of physicochemical properties, mass spectrometry(MS), nuclear magnetic resonance(NMR), Ultraviolet(UV) and infrared(IR) spectroscopic data. These compounds were identified as(1S,2E,4R,5S,7E,11E)-4-methoxy-5-hydroxycembrane(1),(1R~*,4R~*,5E,8E,12E,15E)-4-hydroxycembra-5,8,12,15-tetraene(2), cembrene A(3),(3S,4S,7R)-4-hydroxycembrane(4), and pavidolide D(5). Compound 1 was a new compound. Compounds 2, 4, and 5 were obtained from the gum resin of B. carterii for the first time. Compound 2 showed weak inhibition on the human liver cancer cell line HepG2.


Assuntos
Boswellia , Diterpenos , Linhagem Celular , Humanos , Estrutura Molecular , Resinas Vegetais
6.
Nat Prod Res ; 35(22): 4272-4278, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31813298

RESUMO

Two new isoquinoline alkaloids (1 and 2) along with fourteen known alkaloids (3-16) were isolated from Corydalis racemosa (Thunb.) Pers. Their structures were elucidated by analyzing spectroscopic and spectrometric data (NMR, UV, IR, and MS) and comparing their spectroscopic, spectrometric and physicochemical data with the values archived in the literature. The absolute configurations of new compounds were determined via X-ray crystallographic assay and electronic circular dichroism calculations. Acetylcholinesterase (AChE) inhibitory activity of all compounds was evaluated. Compounds 5, 6, 9, 11, and 12 exhibited inhibitory activity against AChE with IC50 values ranged from 10.2 to 63.4 µM.


Assuntos
Alcaloides , Corydalis , Acetilcolinesterase , Alcaloides/farmacologia , Inibidores da Colinesterase/farmacologia , Dicroísmo Circular , Estrutura Molecular
7.
Nat Prod Res ; 34(14): 2030-2036, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30789078

RESUMO

Two new benzophenone glycosides, aquilarisides A (1) and B (2), together with six known analogues (3-8) were isolated from the pericarps of Aquilaria yunnanensis S. C. Huang. Their structures were elucidated on the basis of 1D and 2D NMR and mass spectroscopic analyses, and the absolute configuration of compound 1 was determined by experimental and calculated electronic circular dichroism (ECD) spectra. Anti-inflammatory activities of all compounds 1-8 were evaluated for their inhibitory activities against lipopolysaccharide (LPS)-stimulated induced nitric oxide (NO) production in RAW 264.7 cells using the Griess assay. Compound 2 indicated a weak inhibition of NO production.


Assuntos
Anti-Inflamatórios/isolamento & purificação , Benzofenonas/química , Glicosídeos/isolamento & purificação , Thymelaeaceae/química , Animais , Anti-Inflamatórios/química , Anti-Inflamatórios/farmacologia , Benzofenonas/isolamento & purificação , Benzofenonas/farmacologia , Glicosídeos/química , Glicosídeos/farmacologia , Lipopolissacarídeos , Espectroscopia de Ressonância Magnética , Espectrometria de Massas , Camundongos , Conformação Molecular , Estrutura Molecular , Óxido Nítrico/antagonistas & inibidores , Óxido Nítrico/biossíntese , Células RAW 264.7
8.
Phytochemistry ; 158: 46-55, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30453219

RESUMO

Fifteen previously undescribed 2-(2-phenylethyl)chromone dimers, along with two known analogues were isolated from Chinese agarwood (Aquilaria sinensis) by a LC-MS-guided fractionation procedure. Their structures were elucidated on the basis of spectroscopic and spectrometric data (1D and 2D NMR, IR, and HRESIMS). The isolated compounds exhibited significant inhibition of nitric oxide production in lipopolysaccharide-stimulated RAW264.7 cells with IC50 values in the range 0.6-37.1 µM.


Assuntos
Anti-Inflamatórios não Esteroides/química , Anti-Inflamatórios não Esteroides/farmacologia , Cromonas/química , Thymelaeaceae/química , Animais , Anti-Inflamatórios não Esteroides/isolamento & purificação , Cromatografia Líquida , Cromonas/isolamento & purificação , Dimerização , Avaliação Pré-Clínica de Medicamentos/métodos , Concentração Inibidora 50 , Lipopolissacarídeos/farmacologia , Espectroscopia de Ressonância Magnética , Camundongos , Estrutura Molecular , Óxido Nítrico/antagonistas & inibidores , Óxido Nítrico/metabolismo , Plantas Medicinais/química , Células RAW 264.7 , Espectrometria de Massas por Ionização por Electrospray
9.
Zhongguo Zhong Yao Za Zhi ; 43(1): 109-113, 2018 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-29552819

RESUMO

Ten phenylpropanoid amides were isolated from the whole plants of Corydalis edulis Maxim. by various of column chromatographies including silica gel, Sephadex LH-20, and ODS. Their structures were identified on the basis of physicochemical properties, MS, NMR, and IR spectroscopic data. These compounds were identified as N-trans-sinapoyl-3-methoxytyramine-4'-O-ß-glucoside(1), N-trans-sinapoyl-3-methoxytyramine(2), N-trans-sinapoyltyramine(3), N-trans-p-coumaroyltyramine(4), N-trans-sinapoyl-7-hydroxytyramine(5), N-cis-feruloyltyramine(6), N-cis-p-coumaroyltyramine(7), N-trans-feruloyltyramine(8), N-trans-feruloyl-3-methoxytyramine(9), and N-trans-feruloyl-7-hydroxytyramine(10). Compound 1 is a new compound. Compounds 2-7 are obtained from the plants of Papaveraceae for the first time, while compounds 8-10 are firstly isolated from C. edulis.


Assuntos
Amidas/análise , Corydalis/química , Glucosídeos/análise , Compostos Fitoquímicos/análise , Tiramina/análise
10.
J Nat Prod ; 81(3): 543-553, 2018 03 23.
Artigo em Inglês | MEDLINE | ID: mdl-29227647

RESUMO

Sixteen new 2-(2-phenylethyl)chromone dimers, including four pairs of enantiomers (1a/1b, 3a/3b, 6a/6b, and 8a/8b), along with eight optically pure analogues (2, 4, 5, 7, and 9-12) were isolated from the resinous wood of Aquilaria sinensis. Their structures were determined by extensive spectroscopic analysis (1D and 2D NMR, UV, IR, and HRMS) and experimental and computed ECD data. Compounds 1-10 feature an unusual 3,4-dihydro-2 H-pyran ring linkage connecting two 2-(2-phenylethyl)chromone monomeric units, while compounds 11 and 12 possess an unprecedented 6,7-dihydro-5 H-1,4-dioxepine moiety in their structures. A putative biosynthetic pathway of the representative structures via a diepoxy derivative of a chromone with a nonoxygenated A-ring is also proposed. Compounds 1a/1b, 2, 3a/3b, 5, 7, 8a/8b, and 10-12 exhibited significant inhibition of nitric oxide production in lipopolysaccharide-stimulated RAW264.7 cells with IC50 values in the range 7.0-12.0 µM.


Assuntos
Anti-Inflamatórios/química , Anti-Inflamatórios/farmacologia , Flavonoides/química , Flavonoides/farmacologia , Thymelaeaceae/química , Madeira/química , Animais , Linhagem Celular , Inibidores da Colinesterase/química , Inibidores da Colinesterase/farmacologia , Lipopolissacarídeos/farmacologia , Espectroscopia de Ressonância Magnética/métodos , Camundongos , Óxido Nítrico/metabolismo , Células RAW 264.7 , Resinas Vegetais/química , Resinas Vegetais/farmacologia
11.
Front Pharmacol ; 8: 281, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28588487

RESUMO

Multiple sclerosis is a chronic inflammatory autoimmune disease of the central nervous system characterized by demyelinating plaques and axonal loss. Inhibition on over activation of innate and adaptive immunity provides a rationale strategy for treatment of multiple sclerosis. In the present study, we investigated the inhibitory effects of GYF-21, an epoxide 2-(2-phenethyl)-chromone derivative isolated from Chinese agarwood, on innate and adaptive immunity for revealing its potential to treat multiple sclerosis. The results showed that GYF-21 markedly inhibited the activation of microglia, and dendritic cells as well as neutrophils, all of which play important roles in innate immunity. Furthermore, GYF-21 significantly suppressed adaptive immunity via inhibiting the differentiation of naive CD4+ T cells into T helper 1 (Th1) and T helper 17 (Th17) cells, and suppressing the activation, proliferation, and IFN-γ secretion of CD8+ T cells. The mechanism study showed that GYF-21 evidently inhibited the activation of STAT1/3 and NF-κB signaling pathways in microglia. In conclusion, we demonstrated that GYF-21 can significantly inhibit innate and adaptive immunity via suppressing STAT1/3 and NF-κB signaling pathways, and has potential to be developed into therapeutic drug for multiple sclerosis.

12.
Fitoterapia ; 118: 49-55, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-28237880

RESUMO

Five new 2-(2-phenylethyl)chromone derivatives (1-5), along with eleven known compounds (6-16) were isolated from Chinese agarwood. Their structures were elucidated by spectroscopic data (NMR, UV, IR, and MS) analyses and comparison of their spectroscopic and physical data with the literature values. The absolute configurations of 2-4 were determined by electronic circular dichroism (ECD) calculations. Compounds 2-4, 11, 12, and 15 exhibited significant inhibition of nitric oxide production in lipopolysaccharide-stimulated RAW 264.7 cells with IC50 values in the range 1.6-7.3µM.


Assuntos
Anti-Inflamatórios/química , Flavonoides/química , Thymelaeaceae/química , Animais , Anti-Inflamatórios/isolamento & purificação , Flavonoides/isolamento & purificação , Camundongos , Estrutura Molecular , Óxido Nítrico/metabolismo , Células RAW 264.7 , Madeira/química
13.
Zhongguo Zhong Yao Za Zhi ; 41(22): 4197-4203, 2016 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-28933089

RESUMO

Chemical constituents from the fruits of Vitex negundo var. cannabifolia and their nitric oxide (NO) inhibitory and cytotoxic activities were investigated. The compounds were isolated and purified by various column chromatography, and their structures were identified by physiochemical properties and spectroscopic data. Thirteen lignans and six phenolic compounds were isolated from the CH2Cl2 extract of the fruits of V. negundo var. cannabifolia, respectively. Their structures were elucidated as 6-hydroxy-4-(4-hydroxy-3-methoxyphenyl)-3-hydroxymethyl-7-methoxy-3,4-dihydro-2-naphthaldehyde (1), vitedoin A (2), vitexdoin F (3), detetrahydroconidendrin (4), vitexdoin E (5), 4-oxosesamin (6), L-sesamin (7), (+)-beechenol (8), ligballinol (9), 2-(4-hydroxyphenyl)-6-(3-methoxy-4-hydroxyphenyl)-3,7-dioxabicyclo[3.3.0]octane (10), (-)-pinoresinol (11), balanophonin (12), thero-guaiacylglycerol-ß-coniferyl aldehyde ether (13), trans-p-coumaryl aldehyde (14), coniferyl aldehyde (15), 5,7-dihydroxychromone (16), trans-3,5-dimethoxy-4-hydroxy-cinnamic aldehyde (17), frambinone (18), and alternariol 4-methyl ether (19). Compounds 8-10,14,18,19 were firstly isolated from Verbenaceae family, compound 13 was obtained from Vitex species, and 6,7,12,15-17 from V. negundo var. cannabifolia for the first time, respectively. The isolated compounds were evaluated for their anti-inflammatory and cytotoxic effects in vitro. Eight compounds (3,5,7,10,11,14,15,17) showed inhibition against NO production in LPS-stimulated RAW 267.4 cells (IC50 in the range of 7.8-81.1 µmol•L⁻¹) and four compounds (1-4) showed cytotoxicity on HepG-2 cells (IC50 in the range of 5.2-24.2 µmol•L⁻¹).


Assuntos
Frutas/química , Compostos Fitoquímicos/isolamento & purificação , Vitex/química , Animais , Células Hep G2 , Humanos , Camundongos , Óxido Nítrico/metabolismo , Compostos Fitoquímicos/farmacologia , Células RAW 264.7
14.
Chin J Nat Med ; 13(9): 699-703, 2015 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-26412430

RESUMO

A new γ-alkylated-γ-butyrolactone, named melongenolide A (1), along with nine known compounds were obtained from the roots of Solanum melongena, and their structures were identified as melongenolide A (1), (+)-syringaresinol (2), (+)-lyoniresinol (3), 5,5'-dimethoxy lariciresinol (4), (+)-(7R,8R)-4-hydroxy-3,3',5'-trimethoxy-8',9'-dinor-8,4'-oxyneoligna-7, 9-diol-7'-aldehyde (5), kaempferol-3-O-(2″,6″-di-O-p-trans-coumaroyl)-ß-glucoside (6), arjunolic acid (7), vanillic acid (8), scoparone (9), and ß-sitosterol (10). Compounds 2, 6, and 7 showed potent inhibitory effects on nitric oxide production in lipopolysaccharide-induced RAW 264.7 macrophages, with IC50 values being 5.62 ± 0.86, 11.47 ± 0.98, and 27.75 ± 1.26 µmol·L(-1), respectively.


Assuntos
4-Butirolactona/análogos & derivados , Extratos Vegetais/química , Raízes de Plantas/química , Solanum melongena/química , 4-Butirolactona/isolamento & purificação , Animais , Furanos/isolamento & purificação , Furanos/farmacologia , Inflamação/tratamento farmacológico , Inflamação/metabolismo , Concentração Inibidora 50 , Quempferóis/isolamento & purificação , Quempferóis/farmacologia , Lignanas/isolamento & purificação , Lignanas/farmacologia , Macrófagos/efeitos dos fármacos , Macrófagos/metabolismo , Camundongos , Óxido Nítrico/metabolismo , Extratos Vegetais/farmacologia , Extratos Vegetais/uso terapêutico , Células RAW 264.7 , Triterpenos/isolamento & purificação , Triterpenos/farmacologia
15.
Fitoterapia ; 106: 115-21, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-26304765

RESUMO

Nine new sesquiterpenes (1-9), together with seventeen known ones (10-26), were isolated from Chinese eaglewood. Their structures were established by extensive spectroscopic analysis, and the absolute configuration of 6 was determined by the modified Mosher's method. Compounds 7, 10, 14, 15, and 21 exhibited significant inhibition of nitric oxide production in lipopolysaccharide-stimulated BV-2 microglial cells with IC50 values in the range 7.1-53.8 µM.


Assuntos
Medicamentos de Ervas Chinesas/química , Microglia/efeitos dos fármacos , Sesquiterpenos/química , Thymelaeaceae/química , Animais , Linhagem Celular , Camundongos , Estrutura Molecular , Óxido Nítrico/metabolismo , Sesquiterpenos/isolamento & purificação , Madeira/química
16.
J Agric Food Chem ; 63(13): 3426-36, 2015 Apr 08.
Artigo em Inglês | MEDLINE | ID: mdl-25796999

RESUMO

Eggplant (Solanum melongena L.) is a famous edible and medicinal plant. Despite being widely cultivated and used, data on certain parts other than the fruit are limited. The present study focused on the qualitative and quantitative analysis of the chemical constituents, particularly phenylpropanoid amides (PAs), in eggplant. The mass fragmentation patterns of PAs were proposed using seven authentic compounds with the assistance of a hybrid ion trap time-of-flight mass spectrometer. Thirty-seven compounds (27 PAs and 10 others) were detected and plausibly assigned in the different parts of eggplant. Afterward, a reliable method based on liquid chromatography coupled with diode array detection was developed, validated, and applied for the simultaneous determination of seven PAs and three caffeoylquinic acids in 17 batches of eggplant roots with satisfactory accuracy, precision, and reproducibility, which could not only provide global chemical insight of eggplant but also offer a reliable tool for quality control.


Assuntos
Amidas/análise , Cromatografia Líquida de Alta Pressão/métodos , Espectrometria de Massas/métodos , Propanóis/análise , Solanum melongena/química , Frutas/química , Medicina Tradicional Chinesa , Folhas de Planta/química , Raízes de Plantas/química , Caules de Planta/química , Controle de Qualidade , Ácido Quínico/análogos & derivados , Ácido Quínico/análise , Reprodutibilidade dos Testes
17.
Zhongguo Zhong Yao Za Zhi ; 40(16): 3224-8, 2015 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-26790297

RESUMO

The flavonoids were investigated from the whole plants of Lagopsis supina. The compounds were isolated and purified by various column chromatography, and their structures were identified by physiochemical properties and spectroscopic data. Two flavones were isolated from the CH2Cl2 layer of Lagopsis supina extract and identified as genkwanin (1) and 5-hydroxy-7,4'-dimethoxyflavone (2), respectively. Ten flavonoid glycosides were isolated from the water layer of Lagopsis supina and elucidated as kaempferol-3-O-6" (3-hydroxy-3-methylglutaryl) -ß-D-glucoside (3), quercetin-3-O-6"-(3-hydroxy-3-methylglutaryl) -ß-D-glucoside (4), quercetin-3-O-ß-D-glucoside(5), kaempferol-3-Of3-D-glucoside ( 6), isorhamnetin-3-O-/-D-glycopyranoside (7), apigenin-7-O-6-D-glucoside (8), luteolin-7-O-ß-D-glucoside (9), chrysoeriol-7-O-ß-D-glucoside (10), rutin (11 ), and kaempferol-3-ß-(6"-p-coumaroyl) -ß-D-glucoside (tiliroside, 12). Compounds 3 and 4 were firstly isolated from Lamiaceae family, and compounds 1-12 were isolated from the plants of Lagopsis genus for the first time.


Assuntos
Medicamentos de Ervas Chinesas/química , Flavonoides/química , Lamiaceae/química , Medicamentos de Ervas Chinesas/isolamento & purificação , Flavonoides/isolamento & purificação , Estrutura Molecular , Espectrometria de Massas por Ionização por Electrospray
18.
Fitoterapia ; 99: 64-71, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25218969

RESUMO

Two new dihydrochalcones, 4-hydroxy-2,4'-dimethoxydihydrochalcone (1) and 3,4'-dihydroxy-2,4,6-trimethoxydihydrochalcone (2), and a new homoisoflavane, 7,3'-dihydroxy-8,4'-dimethoxyhomoisoflavane (3), along with 12 known compounds (4-15), were isolated from the red resin of Dracaena cochinchinensis (Chinese dragon's blood). Their structures were assigned by a variety of spectroscopic techniques. Diversity of cleavage pathways were proposed for dihydrochalcones and homoisoflavanes based on the mass spectroscopic behaviors of those identified compounds using hybrid ion trap-time of flight mass spectrometry. All the compounds were evaluated for their inhibitory effects on nitric oxide production in lipopolysaccharide-induced RAW 264.7 macrophages, and compound 9 exhibited mild inhibition of NO production in this assay with IC50 value of 50.3 µM.


Assuntos
Anti-Inflamatórios/química , Chalconas/química , Dracaena/química , Isoflavonas/química , Resinas Vegetais/química , Animais , Anti-Inflamatórios/isolamento & purificação , Linhagem Celular , Chalconas/isolamento & purificação , Isoflavonas/isolamento & purificação , Macrófagos/efeitos dos fármacos , Camundongos , Estrutura Molecular , Óxido Nítrico/metabolismo
19.
Fitoterapia ; 98: 110-6, 2014 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-25068200

RESUMO

Four new lignanamides, melongenamides A-D (1-4), together with six known ones (5-10), were isolated from the roots of Solanum melongena L. Their structures were elucidated on the basis of 1D and 2D NMR experiments and by comparison of their spectroscopic and physical data with the literature values. Compounds 2-8 exhibited inhibitions of nitric oxide production in lipopolysaccharide-induced RAW 264.7 macrophages with IC50 values ranging from 16.2 to 58.5 µM.


Assuntos
Amidas/química , Anti-Inflamatórios/farmacologia , Lignanas/química , Macrófagos/efeitos dos fármacos , Solanum melongena/química , Amidas/isolamento & purificação , Amidas/farmacologia , Animais , Anti-Inflamatórios/isolamento & purificação , Concentração Inibidora 50 , Lignanas/isolamento & purificação , Lignanas/farmacologia , Camundongos , Estrutura Molecular , Óxido Nítrico/metabolismo , Raízes de Plantas/química
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