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1.
BMJ Open ; 9(9): e031021, 2019 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-31501129

RESUMO

OBJECTIVE: To investigate the current situation of anaemia among children aged 6-23 months in a rural county in China, and to explore the influencing factors and the main causes of anaemia. DESIGN: A cross-sectional study. SETTING: Huzhu County in Qinghai Province, China PARTICIPANTS: We selected 38 sampled villages using Proportional to Population Size sampling method. We obtained the name list of children aged 6-23 months in each sampled village and planned to survey all the eligible children aged 6-23 months and their caregivers. PRIMARY AND SECONDARY OUTCOMES MEASURES: The prevalence of anaemia, the influencing factors of anaemia, the laboratory tests for biological causes of anaemia, including serum ferritin, soluble transferrin receptor, folic acid, homocysteine and vitamin B12. RESULTS: A total of 754 children aged 6-23 months and their caregivers were surveyed, and 183 anaemic children aged 12-23 months were collected venous blood sample. The anaemia prevalence of children aged 6-23 months in Huzhu County was 59.1%. Children of younger age (OR=0.968, 95% CI 0.940 to 0.998), Tibetan nationality (OR=3.123, 95% CI 1.473 to 6.623) and not introducing meat (OR=0.698, 95% CI 0.499 to 0.976) were more likely to be anaemic. More than 80% of children with anaemia were due to iron deficiency (ID), and 20.2% of them had both iron and folic acid deficiencies. CONCLUSIONS: The anaemia prevalence of children aged 6-23 months in Huzhu County was high and children of younger age, Tibetan nationality and not introducing meat were more likely to be anaemic. The main cause of anaemia was nutritional anaemia, with the vast majority being ID. Interventions of feeding counselling and nutrients supplements are appropriate and should be further strengthened. TRIAL REGISTRATION NUMBER: ChiCTRPRC12002444.

2.
Clin Nutr ; 2019 May 23.
Artigo em Inglês | MEDLINE | ID: mdl-31164238

RESUMO

BACKGROUND: Garlic-derived organosulfur compounds have numerous potential benefits (e.g. antioxidant and anti-inflammatory effects) on human health. Although these functions might be related to the onset and progression of muscle strength decline, no studies have explored the relationship between garlic consumption and muscle strength in the general population. The aim of this study was to investigate whether raw garlic consumption is related to handgrip strength in a large-scale adult population. METHODS: A cross-sectional study (n = 28,958) was performed in Tianjin, China. Frequency of raw garlic consumption was assessed using a valid self-administered food frequency questionnaire. Handgrip strength was measured using a handheld digital dynamometer. Analysis of covariance and multiple logistic regression were used to evaluate the relationship between raw garlic consumption and handgrip strength. RESULTS: In males, after adjusted potential confounding factors, the least square means (95% confidence intervals) of handgrip strength across raw garlic consumption categories were 42.5 (40.2-44.9) for almost never; 43.0 (40.7-45.5) for <1 time/week; 43.4 (41.0-45.8) for 1 time/week; and 43.8 (41.5-46.3) for ≥2-3 times/week (P for trend <0.0001). The adjusted odds ratios (95% confidence intervals) of low handgrip strength (<20th percentile of handgrip strength) across the categories of raw garlic consumption in males were 1.00, 0.86 (0.76, 0.98), 0.76 (0.67, 0.87), and 0.66 (0.58, 0.74) (P for trend <0.0001). Similar results were also observed in females. CONCLUSIONS: The study firstly revealed a positive correlation between raw garlic consumption and handgrip strength in both males and females. Further studies are needed to explore the casual relationship.

3.
Wei Sheng Yan Jiu ; 48(2): 200-207, 2019 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-31133095

RESUMO

OBJECTIVE: To explore the status of nutrition and associated factors among elderly in China. METHODS: Based the 2010-2012 China National Nutrition and Health Survey(CNNHS), a total of 34 373 participants aged 60 years and older were included in the analysis. People without weight, height, education level, family income level, drinking, smoking status and lack of physical activity information were excluded. The survey adopted a multi-stage stratified cluster random sampling method proportional to the population. Dietary data were collected by the 24-hour-dietary for 3 days questionnaire. Multivariable logistic regression model was performed to examine the association between sociodemographic characteristics and dietary intake with status of nutrition. RESULTS: The 34 373 subjects aged 60 years and older had a 6. 2% low body weight, 31. 8% overweight and 11. 5% obesity. The prevalent of low body weight of elderly women(6. 1%) was higher than that of elderly men(1. 4%). Women(47. 1%)had a higher overweight/obesity prevalence than men(39. 3%)(OR=1. 19, 95% CI 1. 10-1. 28). Participants living in rural areas had a higher prevalence of low body weight(OR=1. 41, 95% CI 1. 07-1. 86) and a lower overweight/obesity prevalence than urban residents(OR=0. 72, 95% CI 0. 61-0. 86). Education levels was negatively associated with low body weight(P<0. 05), but positively associated with overweight/obesity prevalence(P<0. 05). Physical activity levels were inversely related with low body weightand overweight/obesity(P<0. 05). High intake of wheat was associated with a decreased low body weight and an increased overweight/obesity prevalence(P<0. 05). The prevalence of overweight/obesity was negatively related with high intake of rice and organ meat(P<0. 05). CONCLUSION: Sex, region, education level, household income, smoking status, physical activity level and dietary intake were associated with the status of nutrition among elderly in China.


Assuntos
Grupo com Ancestrais do Continente Asiático/estatística & dados numéricos , Obesidade/epidemiologia , Sobrepeso/epidemiologia , População Rural/estatística & dados numéricos , População Urbana/estatística & dados numéricos , Idoso , Índice de Massa Corporal , Peso Corporal , China/epidemiologia , Estudos Transversais , Registros de Dieta , Exercício , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estado Nutricional , Prevalência , Fatores Sexuais , Fatores Socioeconômicos
4.
Wei Sheng Yan Jiu ; 48(1): 49-55, 2019 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-31032767

RESUMO

OBJECTIVE: To study the effect of nutritional intervention for Kazak's pregnant women, lactating women and infants in farming and stockbreeding region of Jeminay County, Altay City. METHODS: 24 h record method was conducted to implement dietary survey, and results were used to analyze dietary structure and nutrient intake level of pregnant women, lactating women and infants. Pregnant women, lactating women and infants over 6 months were intervened with iron fortified soy sauce nutrients supplement and Yingyangbao(YYB) for 2. 4 years. Hemoglobin was detected for pregnant women, lactating women and infants by using HemoCue analyzer. RESULTS: The nutrient intakes of the pregnant women, lactating women and infants were averagely lower than that of the recommended levels. In these infants who received breast feeding, the least acceptability diet quality rate was 42. 1%, and in other infants who didn't receive breast feeding, the rate was 25%. After intervention, anemia prevalence of pregnant women, lactating women and infants were significantly reduced compared with the base line levels at 2 survey time points(2014:pregnant women χ~2=26. 27, lactating women χ~2=18. 06, infants χ~2=44. 46, 2015:pregnant women χ~2=35. 62, lactating women χ~2=25. 05, infants χ~2=39. 61;all P<0. 001). CONCLUSION: The nutrition intervention of whole covering could improve nutrition status of Kazak's pregnant women, lactating women and infants.


Assuntos
Aleitamento Materno , Lactação , Estado Nutricional , Agricultura , Feminino , Humanos , Lactente , Cazaquistão , Gravidez
5.
Nutr Res ; 63: 21-33, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30824394

RESUMO

Ying Yang Bao (YYB), a soybean powder-based complementary food supplement, has been widely used in infants and young children (IYC) for nutrition improvement in poor rural regions in China. This study aims to assess the nutritional effect of YYB on anemia and growth status of IYC between the ages of 6 and 24 months using systematic review and meta-analysis. In this meta-analysis, structured electronic searches were conducted using Medline, PubMed, Cochrane Library, and China National Knowledge Internet datasets. Inclusion criterion, quality assessment, and data extraction were performed by 2 independent reviewers. Data-including hemoglobin concentration; anemia rate; and z scores of height for age, weight for age, and weight for height-were extracted and then analyzed using RevMan 5 software. Seventeen studies on YYB intervention, which showed significantly higher hemoglobin concentration (P < .01) and lower anemia rate (P < .01) in the intervention group compared to the control group, were included. In before-after studies, the prevalence of stunting (P < .01), underweight (P < .01), and wasting (P < .01) significantly decreased, whereas the z scores of weight for height increased (P < .01). However, no significant effect was observed on the z scores of height for age and weight for age. The hemoglobin concentration was higher after using YYB, and the prevalence of anemia, stunting, underweight, and wasting of infants and young children decreased in poor rural regions in China. Based on this meta-analysis, it appears that more randomized controlled trials for YYB intervention should be conducted and the mechanisms explored in future research.

6.
Clin Endocrinol (Oxf) ; 90(5): 753-765, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30776142

RESUMO

OBJECTIVE: Handgrip strength (HGS) begins an accelerating decline around 50 years. Many of the studies performed in old adults have demonstrated a significant relationship between vitamin D and HGS, but the studies performed in participants with a broad age range have yielded conflicting results. The purpose of the study was to investigate the relationship between vitamin D and HGS using age 50 as a specific cut-off. DESIGN: Population-based, cross-sectional study. PARTICIPANTS: Totally 5102 participants (2911 males, 2191 females) from the TCLSIH Cohort. MEASUREMENTS: Serum concentration of 25-hydroxyvitamin D (25(OH)D) was measured using an enzyme immunoassay. We divided participants into quartiles according to 25(OH)D, and the ranges for increasing quartiles were as follows: (males [≥50 years]: 10.94-31.85, 31.88-43.01, 43.20-56.06, 56.20-143.0; males [<50 years]: 11.11-34.68, 34.71-46.91, 46.96-59.45, 59.50-143.7; females [≥50 years]: 7.21-30.01, 30.02-40.18, 40.21-52.44, 52.49-275.4; females [<50 years]: 5.29-28.91, 28.92-40.19, 40.20-51.90, 51.91-140.2). HGS was measured with a hydraulic hand-held dynamometer. Analysis of covariance was employed to explore the relationship. RESULTS: Among males aged above 50 years, the means (95% confidence interval) for HGS per body weight across the categories of serum 25(OH)D concentration were 0.523 (0.430-0.638), 0.545 (0.447-0.664), 0.543 (0.446-0.661), 0.546 (0.449-0.664) (Ptrend  < 0.01) after adjustment for potential confounding factors. However, no relationships were observed between serum 25(OH)D concentration and HGS in males aged below 50 years and females in the whole age range. CONCLUSIONS: Serum 25(OH)D concentration was significantly related to HGS in males aged above 50 years, independent of confounding factors. Future studies are needed to clarify the age and sex relationship between serum 25(OH)D concentration and HGS.

7.
Metabolism ; 93: 61-67, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30690038

RESUMO

BACKGROUND: Muscle weakness is followed by insulin resistance which is associated with metabolic disorders leading to metabolic syndrome (MetS). Therefore, muscle strength decline may be associated with MetS. The aim of this study was to investigate the relationships between muscle strength and MetS and its separate components. METHODS: A cross-sectional study was performed in 17,703 participants aged 40 years and older living in Tianjin, China. Handgrip strength was measured using a handheld digital dynamometer. MetS was defined in accordance with the criteria of the American Heart Association scientific statements of 2009. Multiple logistic regression analyses were used to assess the association between handgrip strength and MetS and its separate components. RESULTS: The overall prevalence of MetS was 33.6%. The prevalence of MetS was significantly higher in men than in women (41.6% vs 22.9%, P < 0.0001). After adjustment for potential confounding factors (including sociodemographic variables, lifestyle factors, total energy intake, and family history of disease), the odds ratios (95% confidence interval) of MetS across decreasing handgrip strength quartiles were: 1.00 (reference), 1.87 (1.66, 2.11), 2.40 (2.13, 2.71), and 3.36 (2.97, 3.80) in men and 1.00 (reference), 1.80 (1.48, 2.21), 2.77 (2.29, 3.36), and 3.89 (3.22, 4.71) in women, respectively (P for all trend <0.0001). Similarly, handgrip strength was also observed to be negatively associated with separate components of MetS both in men and women. CONCLUSIONS: Muscle strength is inversely associated with MetS and its separate components. Further prospective studies are needed to confirm this issue.

9.
FASEB J ; 33(4): 4688-4702, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30592646

RESUMO

Folate deficiency in early development leads to disturbance in multiple processes, including neurogenesis during which fibroblast growth factor (FGF) pathway is one of the crucial pathways. Whether folic acid (FA) directly affects FGF pathways to influence neurodevelopment and the possible mechanism remains unclear. In this study, we presented evidence that in human FA-insufficient encephalocele, the FGF pathway was interfered. Furthermore, in Brachyury knockout mice devoid of such T-box transcription factors regulating embryonic neuromesodermal bipotency and a key component of FGF pathway, change in expression of Brachyury downstream targets, activator Fgf8 and suppressor dual specificity phosphatase 6 was detected, along with the reduction in expression of other key FGF pathway genes. By using a FA-deficient cell model, we further demonstrated that decrease in Brachyury expression was through alteration in hypermethylation at the Brachyury promoter region under FA deficiency conditions, and suppression of Brachyury promoted the inactivation of the FGF pathway. Correspondingly, FA supplementation partially reverses the effects seen in FA-deficient embryoid bodies. Lastly, in mice with maternal folate-deficient diets, aberrant FGF pathway activity was found in fetal brain dysplasia. Taken together, our findings highlight the effect of FA on FGF pathways during neurogenesis, and the mechanism may be due to the low expression of Brachyury gene via hypermethylation under FA-insufficient conditions.-Chang, S., Lu, X., Wang, S., Wang, Z., Huo, J., Huang, J., Shangguan, S., Li, S., Zou, J., Bao, Y., Guo, J., Wang, F., Niu, B., Zhang, T., Qiu, Z., Wu, J., Wang, L. The effect of folic acid deficiency on FGF pathway via Brachyury regulation in neural tube defects.

10.
Wei Sheng Yan Jiu ; 47(5): 733-740, 2018 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-30593301

RESUMO

OBJECTIVE: To study on the cost-benefit from anemia reduction of 6-24 months infants in poor rural regions by Ying Yang Bao( YYB) intervention. METHODS: YYB was donated and distributed to 6-24 months infants in 8 counties in provinces of Sichuan, Gansu and Shaanxi. Total covered infants were 25 125 within them 1200 were sampled and measured hemoglobin levels and change of anemia prevalence in the baseline and after 1 year intervention. Total recovered infants number from anemia was calculated with decrease of anemia rate. The cost-benefit was analyzed with PROFILING and DALY models. RESULTS: The total cost estimated was 6 134 871 RMB, average cost per person was 244 RMB among which costs of YYB, administration and manpower were 121 RMB, 104 RMB and 19 RMB respectively. After 1 year intervention, the anemia rate significantly declined from 52. 8 % to 26. 3 %. The recovered DALYs from present anemia and future cognition was 4863. 08 person·year. Benefit from 1 year YYB intervention in the counties was 69. 3065 million RMB from PROFILING model and60. 6874 million RMB from DALY model. The average cost of the intervention was 938. 46 RMB in saving 1 anemic and 1261. 52 RMB in saving 1 DALY loss in YYB intervention project. The cost-benefit ratio was 1 ∶ 10 at least. Sensitivity analysis showed stability of this cost-benefit analysis acceptable. CONCLUSION: The nutrition intervention using YYB for poor rural region showed high economic benefit.


Assuntos
Saúde do Lactente , Estado Nutricional , População Rural , Anemia , China , Análise Custo-Benefício , Humanos , Lactente , Pesquisa
11.
Wei Sheng Yan Jiu ; 47(6): 883-889, 2018 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-30593316

RESUMO

OBJECTIVE: To analysis the associations between the polymorphisms of TMPRSS6 and the levels of serum ferritin( SF) and soluble transferrin receptor( s TfR) in pregnant women. METHODS: A total of 807 pregnant women were recruited by using cluster random sampling method from Lüliang in Shanxi Province in 2014. Roche Tinaquant immunoturbidimetric assay was used to measure the level of SF and s TfR, then iron deficiency( ID) was identified by the criteria of SF < 25 ng/m L and s TfR > 4. 4 mg/L, respectively. Sequenom MassArray was used to genotype the 7 targeted single-nucleotide polymorphisms( SNPs)( rs11704654, rs1421312, rs2111833, rs2235321, rs2543519, rs4820268 and rs855791) of the subjects. The t test and ANOVA analysis were used to test the different levels of SF and s TfR among SNPs, and chi-square test and Logistic regression were conducted to detect the associations between genotypes of each locus and ID. RESULTS: The differences of the levels of Ln SF between genotypes in rs2111833 were significant( F = 3. 57, P = 0. 0287), and the Ln SF level of T allele carrier group was lower than CC group( t = 2. 03, P = 0. 0429). The Ln SF level of A allele carrier group was lower than GG carrier group in rs855791( t = 1. 97, P = 0. 0490). For rs11704654, the ratio of SF < 25 ng/m L of T allele carriers was higher than CC carriers( χ~2= 4. 5456, P = 0. 0330). For rs211183, the ratio of SF < 25 ng/m L of T allele carriers was higher than CC carriers( χ~2= 4. 6431, P = 0. 0312). For rs855791, the ratio of SF < 25 ng/m L of GG carriers was lower than A allele carriers( χ~2= 5. 0134, P = 0. 0263). rs11704654( T) and rs855791( A) were still shown the association with SF < 25 ng/m L status in logistic analysis adjusted by age and gestational weeks. The Ln s TfR level of T allele carrier group in rs11704654 was higher than CC carrier group( t =-2. 012, P = 0. 024), and the Ln s TfR level of G allele carrier group in rs2543519 was higher than AA carrier group( t =-1. 954, P = 0. 011). CONCLUSION: The associations between polymorphisms of TMPRSS6 and the levels of SF and s TfR are observed in pregnant women.


Assuntos
Ferritinas , Proteínas de Membrana , Polimorfismo de Nucleotídeo Único , Receptores da Transferrina , Serina Endopeptidases , Alelos , Feminino , Ferritinas/sangue , Humanos , Proteínas de Membrana/genética , Gravidez , Receptores da Transferrina/sangue , Serina Endopeptidases/genética
12.
Wei Sheng Yan Jiu ; 47(6): 913-917, 2018 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-30593327

RESUMO

OBJECTIVE: To investigate the nutritional and growth status of Yingyangbao used population in middle region of China. METHODS: Infants and young children were selected from Anhui, Henan, Hubei, Hunan, Jiangxi and Shanxi Provinces by multistage sampling, probability proportional to size sampling and random equidistant sampling method. There were 8245 infants and young children involved in this survey. The children were surveyed by questionnaire and their body length, body weight and hemoglobin were measured to reveal the growth and anemia status. RESULTS: Among the surveyed population, the stunting rate was 5. 9%, the wasting rate was 2. 6%, and the underweight rate was 3. 4%. The total anemia prevalence was 20. 2%, while mild anemia rate was 19. 1%, moderate anemia rate was 1. 1%, severe anemia was not seen. The rate of effective utilization of Yingyangbao was 73. 7%, which showed increase tendency with age. CONCLUSION: The intervention of Yingyangbao could contribute to the improvement of infant malnutrition and anemia, and promote the physical development. Therefore, Yingyangbao is worthy of continuous promotion in this area.


Assuntos
Desenvolvimento Infantil , Estado Nutricional , Anemia , Estatura , Peso Corporal , Pré-Escolar , China , Humanos , Lactente , Prevalência
13.
Nat Commun ; 9(1): 3436, 2018 08 24.
Artigo em Inglês | MEDLINE | ID: mdl-30143612

RESUMO

Neural tube defects (NTDs) are serious congenital malformations. Excessive maternal homocysteine (Hcy) increases the risk of NTDs, while its mechanism remains elusive. Here we report the role of histone homocysteinylation in neural tube closure (NTC). A total of 39 histone homocysteinylation sites are identified in samples from human embryonic brain tissue using mass spectrometry. Elevated levels of histone KHcy and H3K79Hcy are detected at increased cellular Hcy levels in human fetal brains. Using ChIP-seq and RNA-seq assays, we demonstrate that an increase in H3K79Hcy level down-regulates the expression of selected NTC-related genes including Cecr2, Smarca4, and Dnmt3b. In human NTDs brain tissues, decrease in expression of CECR2, SMARCA4, and DNMT3B is also detected along with high levels of Hcy and H3K79Hcy. Our results suggest that higher levels of Hcy contribute to the onset of NTDs through up-regulation of histone H3K79Hcy, leading to abnormal expressions of selected NTC-related genes.

14.
Wei Sheng Yan Jiu ; 47(2): 312-317, 2018 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-29903290

RESUMO

OBJECTIVE: To explore the association between maternal MTHFR gene polymorphism( C677T) and neural tube defects in offspring through Meta-analysis in China. METHODS: CNKI, Pub Med, Web of Science, Chinese Wan Fang Data databases, CBM, VIP for published articles were searched from the time of Database establishment to July 5 th 2017. The search strategy was based on combinations of the English and/or Chinese keywords, 'MTHFR'and 'folate pathway'and 'polymorphism'or 'SNP'and'NTDs or Neural Tube Defects'. References of reviews and retrieved studies were also scanned. All the case-control studies about MTHFR gene C677T polymorphism and susceptibility of neural tube defect were collected, which were fulfilled the followinginclusion criteria: case-control study and cohort study design, presentation of data necessary for calculating odds ratios( ORs). Data were extracted from studies and analyzed by Rev Man 5. 3 software. RESULTS: A total of 13 papers were selected, including1500 patients and 1654 controls. Meta-analysis result showed that the combined odds ratio values of neural tube defect for offspring with maternal TT, TT + CT and T allele genotypes were 1. 94, 1. 65 and 1. 39, respectively. CONCLUSION: The present Meta-analysis suggests that MTHFR C677T is significantly associated with maternal risk for NTDs in the Chinese population, supplemental folic acid supplementation based on MTHFR polymorphisms will be an important means to further reduce the birth defects of newborns.

15.
PLoS One ; 13(5): e0196576, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29723227

RESUMO

BACKGROUND: Different laboratory methods are used to quantify ferritin concentrations as a marker of iron status. A systematic review was undertaken to assess the accuracy and comparability of the most used methods for ferritin detection. METHODS AND FINDINGS: National and regional databases were searched for prospective, retrospective, sectional, longitudinal and case-control studies containing the characteristics and performance of at least one method for serum/plasma ferritin determinations in humans published to date. The analysis included the comparison between at least 2 methods detailing: sensitivity, precision, accuracy, predictive values, inter-methods adjustment, and use of international reference materials. Pooled method performance was analyzed for each method and across methods. OUTCOMES: Search strategy identified 11893 records. After de-duplication and screening 252 studies were assessed, including 187 studies in the qualitative analysis and 148 in the meta-analysis. The most used methods included radiometric, nonradiometric and agglutination assays. The overall within-run imprecision for the most reported ferritin methods was 6.2±3.4% (CI 5.69-6.70%; n = 171), between-run imprecision 8.9±8.7% (CI 7.44-10.35%; n = 136), and recovery rate 95.6% (CI 91.5-99.7%; n = 94). The pooled regression coefficient was 0.985 among all methods analyzed, and 0.984 when comparing nonradiometric and radiometric methods, without statistical differences in ferritin concentration ranging from 2.3 to 1454 µµg/L. CONCLUSION: The laboratory methods most used to determine ferritin concentrations have comparable accuracy and performance. Registered in PROSPERO CRD42016036222.


Assuntos
Análise Química do Sangue/métodos , Ferritinas/sangue , Análise Química do Sangue/normas , Análise Química do Sangue/estatística & dados numéricos , Ferritinas/normas , Humanos , Ferro/sangue , Limite de Detecção , Plasma/química , Padrões de Referência , Soro/química
16.
Artigo em Inglês | MEDLINE | ID: mdl-29421441

RESUMO

A sensitive method for the simultaneous analysis of vitamin A (VA), 25-hydroxyl vitamin D3 (25-OH VD3) and α-tocopherol (VE) in children plasma by liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed and validated. Sample preparation chose the solid phase extraction. 100 µL of plasma was mixed with 300 µL ethanol contained 4 µL isotope-labelled analytes. After a series operation, the supernatant was applied to the solid phase extraction (SPE) plate (HLB µElution plate). The eluate was evaporated, and reconstituted in 100 µL methanol. And then, 6 µL reconstituted sample was injected into LC-MS/MS. Quantitative analysis was carried out by multiple reaction monitoring (MRM) with a positive mode electrospray (ESi + ). Separations of VA, 25-OH VD3 and VE were performed on an Acquity UPLC reversed-phase Phenyl-Hexyl analytical column (CSH, 2.1 × 50 mm, 1.7 µm). Gradient elution was used at a flow rate of 0.4 mL/min with a mobile phase consisting of 0.1% formic acid solution and 0.1% formic acid, 5 mM ammonium formate in acetonitrile. The total time of analysis was 10 min. The method had a limit of quantification (LOQ) of 10.03, 1.20, and 0.04 ng/mL for VA, 25-OH VD3 and VE in methanol, respectively. The linear calibration curves were fitted over the range of 0.14-14.32 µg/mL, 1.80-180.29 ng/mL, and 6.03-602.99 ng/mL for VA, 25-OH VD3 and VE in methanol. The correlation coefficients were greater than 0.998 for all analytes. The recoveries for all analytes were between 80 and 120% with the inter- and intra-day precisions (presented as relative standard deviation, RSD%) less than 10.0%. Analysis of VA, 25-OH VD3 and VE in recurrent respiratory tract infection children plasma and anemic infants' fingertip blood was then carried out using this method and statistical analysis of the data with statistic package for social science 20.0 (SPSS 20.0). Using this method, multiple fat-soluble vitamins could be detected at the same time. Solid phase extraction was used to simplify sample pretreatment. µElution plate used here could reduce the sample volume, only 100 µL sample was used in this method, and 6 µL reconstituted sample was injected into LC-MS/MS. This makes the method appropriate for larger sample pretreatment, and suitable for children, especially infants and newborns' sample detection, in whom the circulation blood was low.


Assuntos
Colecalciferol/análise , Cromatografia Líquida de Alta Pressão/métodos , Espectrometria de Massas em Tandem/métodos , Vitamina A/análise , alfa-Tocoferol/análise , Calibragem , Criança , Colecalciferol/sangue , Humanos , Limite de Detecção , Extração em Fase Sólida/métodos , Vitamina A/sangue , alfa-Tocoferol/sangue
17.
Asia Pac J Clin Nutr ; 26(6): 1170-1178, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28917245

RESUMO

BACKGROUND AND OBJECTIVES: Associations between genetic variants in the hepcidin regulation pathway and iron status have been reported in previous studies. Most of these studies were conducted in populations of European descent and relatively few studies have been conducted in Chinese populations. In this study, we evaluated associations between single-nucleotide polymorphisms (SNPs) in the hepcidin regulation pathway, serum ferritin (SF) and soluble transferrin receptor (sTfR) in Chinese adolescents. METHODS AND STUDY DESIGN: In total, 692 students from rural boarding schools were selected from six cities in China. The participants were divided into case and control groups according to criteria for SF and sTfR. Furthermore, 33 SNPs in TMPRSS6, TF, TFR2, BMP2, BMP4, HJV, CYBRD1, HFE, IL6, PCSK7, HAMP, KIAA1468, and SRPRB were selected. Associations between the genetic variants and SF or sTfR were detected. RESULTS: For SF, rs4820268 in TMPRSS6 was associated with an SF <25 ng/mL status. Carriers of the G/G genotype of rs4820268 exhibited significantly lower SF levels than A allele carriers did (p=0.047). For sTfR, rs1880669 in TF, rs4901474 in BMP4, and rs7536827 in HJV were significantly associated with an sTfR >=4.4 mg/L status. However, in general linear model analysis, after adjustment for age, sex, and location, only rs1880669 exhibited a stable association with higher sTfR levels (p=0.032). CONCLUSIONS: We found rs4820268, in TMPRSS6 that was associated with a low SF level, as previously reported, and a new association between 1880669 in TF and sTfR.

18.
Nestle Nutr Inst Workshop Ser ; 87: 131-138, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28315893

RESUMO

Ying Yang Bao (YYB), a complementary food supplement, has helped to rapidly improve the nutrition status of infants and young children in poor rural regions in China. The first YYB study was performed in 2001 by the International Life Sciences Institute, the Chinese Centers for Disease Control and Prevention, and Nestlé. In 2008, the establishment of national standards for complementary food supplements stimulated implementation of about 15 nutrition intervention projects in poor rural counties in which YYB was applied to infants and young children as home fortification for complementary feeding. Data were accumulated in different studies and showed that YYB enhanced the growth of infants with respect to both anthropometry and cognition, and decreased anemia prevalence significantly in infants aged 6-24 months. The Chinese government has launched a project named Improving Children's Nutrition in Poor Rural Regions in 2012. The project has covered more than 4 million infants aged 6-24 months in 341 counties in 21 provinces in western and middle regions by the end of 2015. This nutrition intervention in early life is rather an opportunity for a better later life. The YYB program in China might present an example for developing countries aiming to improve nutrition in early life.


Assuntos
Anemia Ferropriva/dietoterapia , Anemia Ferropriva/epidemiologia , Alimentos Fortificados , Fenômenos Fisiológicos da Nutrição do Lactente , Grupo com Ancestrais do Continente Asiático , Desenvolvimento Infantil , Pré-Escolar , China , Cognição , Proteínas na Dieta/administração & dosagem , Suplementos Nutricionais , Humanos , Lactente , Fórmulas Infantis/química , Micronutrientes/administração & dosagem , Estado Nutricional , Prevalência , Ensaios Clínicos Controlados Aleatórios como Assunto , Recomendações Nutricionais , População Rural , Fatores Socioeconômicos
19.
Oncotarget ; 8(67): 110797-110810, 2017 Dec 19.
Artigo em Inglês | MEDLINE | ID: mdl-29340017

RESUMO

Disturbed epigenetic modifications have been linked to the pathogenesis of Neural Tube Defects (NTDs) in those with folate deficiency during pregnancy. However, evidence is lacking to delineate the critical region in epigenome regulated by parental folic acid and mechanisms by which folate deficiency affects normal embryogenesis. Our data from clinical samples revealed the presence of aberrant DNA methylation in GNAS imprinting cluster in NTD samples with low folate concentrations. Results from mouse models indicated that the establishment of GNAS imprinting was influenced by both maternal and paternal folate-deficient diets. Such aberrant GNAS imprinting was present prior to the gametogenesis period. Imprinting in Exon1A/GNAS gDMR was abolished in both spermatozoa and oocytes upon treating with a parental folate-deficient diet (3.6% in spermatozoa, 9.8% in oocytes). Interestingly, loss of imprinting in the GNAS gene cluster altered chromatin structure to an overwhelmingly open structure (58.48% in the folate-free medium group vs. 39.51% in the folate-normal medium group; P < 0.05), and led to a disturbed expression of genes in this region. Furthermore, an elevated cyclic AMP levels was observed in folate acid deficiency group. Our results imply that GNAS imprinting plays major roles in folic acid metabolism regulation during embryogenesis. Aberrant GNAS imprinting is an attribute to NTDs, providing a new perspective for explaining the molecular mechanisms by which folate supplementation in human pregnancy provides protection from NTDs.

20.
Wei Sheng Yan Jiu ; 46(1): 78-83, 2017 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-29903156

RESUMO

OBJECTIVE: To optimize the conditions of protein chip assay for bovine milk ß-Lactoglobulin( ß-Lg). METHODS: A microarrayer was used for printing anti-ß-Lg as antibody I on each 3-dimensional-slide, another antis ß-Lg antibody was used as detection antibody II and goat antibody coupled to Cy3 was used as antibody III. The standard ß-Lg was detected by double antibody sandwich technique. RESULTS: Mouse monoclonal ß-Lg antibody66# was chosen as the probe and contact printing as the printing method. The range between 42 and 92 spots was chosen as the basic printing condition. The concentration of ß-Lg probes was 0. 5 mg / mL. The ß-Lg detection antibody titre was 1∶2000. One percent no protein blocking solution was choosen as the blocking buffer. The lower detection limit and the biological detection limit of ß-Lg were 17. 54 ng / m L and 55. 31 ng / m L respectively. The linear range was determined according to the S type curve of ß-Lg and the best fitting models and standard curve were established for ß-Lg( R~2=0. 9993). CONCLUSION: The study optimizes conditions of a quantitative analysis system for measurement of ß-Lg with protein chip, thus establishing the protein chip platform for quantitative detection of bovine milk ß-Lactoglobulin.


Assuntos
Lactoglobulinas/isolamento & purificação , Leite/química , Análise Serial de Proteínas/métodos , Animais , Bovinos , Lactoglobulinas/análise , Lactoglobulinas/genética , Limite de Detecção , Camundongos , Proteínas do Leite/química
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