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1.
BMC Res Notes ; 14(1): 12, 2021 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-33413567

RESUMO

OBJECTIVE: The current study aimed to investigate the ABO and rhesus (Rh) blood group frequency in the people of District Faisalabad and Sheikhupura, Punjab Province, Pakistan. The retrospective study was conducted on more than thirty thousand people including both male and female patients admitted to the Tehsil Headquarter Hospital, Safdarabad and The Best Hospital, Faisalabad. Blood samples were taken from each subject and subsequently ABO and Rh blood groups were evaluated separately. The antigen antibody agglutination slide test for blood grouping (ABO) and Rh were used to assess the blood group frequencies. RESULTS: The frequencies of ABO blood group distribution indicated that blood group B was predominant in the people of Safdarabad followed by O, A and AB respectively. While, among people of Faisalabad, blood group O was predominant followed B, A and AB respectively. Rh negative phenotype was found lesser distributed as compared to the positive Rh phenotype.

2.
Cureus ; 12(12): e11867, 2020 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-33282609

RESUMO

Objective To evaluate the association of chronic low back pain with levels of vitamin D in the affected population. Methodology This observational study was carried out from August 2016 to August 2019 at Khairpur Medical College and Shaheed Mohatarma Benazir Bhutto Medical College, Karachi, Pakistan. Patients aged 18 years and above suffering from chronic low back pain with pain persisting for more than 12 weeks were the study participants after written consent and prior approval from the ethical review committee was obtained for conducting the study. Data was recorded on predesigned performa and analyzed on SPSS Version 20 (IBM Corp.). Results There were 1,152 cases with chronic lower back pain, of whom 632 (54.9%) were females and 520 (45.1%) were males. The mean age of the patients was 41.76 ± 11.18 years. The mean visual analog scale (VAS) level was 5.36 ± 1.65; 707 cases (61.4%) had moderate pain according to VAS, 292 (25.3%) had severe pain, and 153 (13.3%) had mild pain. Concerning vitamin D levels, the mean levels were 22.74 ± 13.80, with 599 (52%) of the patients having deficient levels of vitamin D, 347 (30.1%) having insufficient levels, and only 204 (17.7%) of the cases having normal vitamin D levels. Conclusions Lower back pain is one of the common presenting problems in orthopedic clinics. We found no relationship between chronic lower back pain and vitamin D levels in our study.

3.
Cureus ; 12(10): e11271, 2020 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-33274146

RESUMO

Objective This study's main purpose is to determine the functional outcome of volar locking plates in the management of intraarticular fracture of the distal radius. Methodology This cross-sectional study was conducted from August 2016 to August 2019. Forty-nine patients with the intraarticular distal radius fractures managed by the volar locking plate were included in the study. Patients with open fractures, ipsilateral other limb injuries, polytrauma patients, pathological fractures, and patients having neurovascular injuries were excluded from the study. AO Classification was used to classify fractures. The fracture was approached through the volar approach and fixed by the volar locking plate. A modified mayo wrist score evaluated the functional outcome. All the data were recorded on predesigned performa, and Statistical Package for the Social Sciences (SPSS), version 20 (IBM Corp., Armonk, NY) was used to analyze the data. Results A total of 49 patients with intraarticular fractures of the distal radius were included in the study. The mean age of the patient was 37.20 ± 10.05 years. Out of 49 patients, 29 (59.2%) were males, and 20 (40.8%) were females. Union was achieved in almost all fractures except one case, which went into non-union despite the adequate initial reduction, and the mean time of union was 11.98 ± 1.64 weeks. With respect to the stratification of functional outcome very good and good functional outcome was achieved in 46 patients (93.8%) in both the groups, the satisfactory outcome was achieved in two (4.1%) cases and one patient had a bad outcome in which union was not achieved and went into non-union. Conclusion Open reduction and internal fixation of intraarticular fracture of the distal radius using a volar locking plate is a good option for managing these fractures as it provides stable fixation, and good to excellent outcomes can be achieved by using these plates.

4.
Biomolecules ; 10(12)2020 Dec 18.
Artigo em Inglês | MEDLINE | ID: mdl-33353010

RESUMO

The impact of heavy metal, i.e., cadmium (Cd), on the growth, photosynthetic pigments, gas exchange characteristics, oxidative stress biomarkers, and antioxidants machinery (enzymatic and non-enzymatic antioxidants), ions uptake, organic acids exudation, and ultra-structure of membranous bounded organelles of two rice (Oryza sativa L.) genotypes (Shan 63 and Lu 9803) were investigated with and without the exogenous application of ferrous sulfate (FeSO4). Two O. sativa genotypes were grown under different levels of CdCl2 [0 (no Cd), 50 and 100 µM] and then treated with exogenously supplemented ferrous sulfate (FeSO4) [0 (no Fe), 50 and 100 µM] for 21 days. The results revealed that Cd stress significantly (p < 0.05) affected plant growth and biomass, photosynthetic pigments, gas exchange characteristics, affected antioxidant machinery, sugar contents, and ions uptake/accumulation, and destroy the ultra-structure of many membranous bounded organelles. The findings also showed that Cd toxicity induces oxidative stress biomarkers, i.e., malondialdehyde (MDA) contents, hydrogen peroxide (H2O2) initiation, and electrolyte leakage (%), which was also manifested by increasing the enzymatic antioxidants, i.e., superoxidase dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX) and non-enzymatic antioxidant compounds (phenolics, flavonoids, ascorbic acid, and anthocyanin) and organic acids exudation pattern in both O. sativa genotypes. At the same time, the results also elucidated that the O. sativa genotypes Lu 9803 are more tolerant to Cd stress than Shan 63. Although, results also illustrated that the exogenous application of ferrous sulfate (FeSO4) also decreased Cd toxicity in both O. sativa genotypes by increasing antioxidant capacity and thus improved the plant growth and biomass, photosynthetic pigments, gas exchange characteristics, and decrease oxidative stress in the roots and shoots of O. sativa genotypes. Here, we conclude that the exogenous supplementation of FeSO4 under short-term exposure of Cd stress significantly improved plant growth and biomass, photosynthetic pigments, gas exchange characteristics, regulate antioxidant defense system, and essential nutrients uptake and maintained the ultra-structure of membranous bounded organelles in O. sativa genotypes.

5.
Microb Pathog ; 150: 104675, 2020 Dec 24.
Artigo em Inglês | MEDLINE | ID: mdl-33352217

RESUMO

Human gut microbiota consists of various microorganisms whose numbers are similar to those of human cells. Human gut microbes and the brain form bidirectional communications through the brain-gut-axis, and play a central role in normal physiological processes and in pathogenesis of many human diseases. Accumulating evidence has demonstrated the crucial effect of gut microbes in proper brain functions and under disease conditions. Here we first focus on revealing current knowledge of the role of gut microbes in neural development and functions. We then summarize mutual relationships between gut microbes and human diseases, in particular neurodegenerative disorders such as Alzheimer's disease, Parkinson's disease and Multiple sclerosis. Finally, we highlight ongoing studies in exploring gut microbes in treatments of human diseases. Applying gut microbes as a means in treatment of human diseases is becoming a promising research direction, and has a great potential in clinical practice.

6.
Chaos ; 30(10): 103104, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-33138453

RESUMO

This paper describes the existence and stability of the hepatitis B epidemic model with a fractional-order derivative in Atangana-Baleanu sense. Some new results are handled by using the Sumudu transform. The existence and uniqueness of the equilibrium solution are presented using the Banach fixed-point theorem. Moreover, sensitivity analysis complemented by simulations is performed to determine how changes in parameters affect the dynamical behavior of the system. The numerical simulations are carried out using a predictor-corrector scheme to demonstrate the obtained results.

7.
J Biol Dyn ; 14(1): 788-801, 2020 12.
Artigo em Inglês | MEDLINE | ID: mdl-33073738

RESUMO

In this article, a stochastic SACR hepatitis B epidemic model is taken to be under consideration. We develop a stochastic epidemic model by considering the effect of environmental fluctuation on the hepatitis B dynamics and distribute the transmission rate by white noise. Using the stochastic Lyapunov function theory, we have shown the existence and uniqueness of the global positive solution. The extinction and persistence for our proposed model are derived with sufficient conditions. The numerical simulations are carried out using first-order Itô-Taylor stochastic scheme in the last section for the verification of our theoretical results.

8.
Plants (Basel) ; 9(10)2020 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-33080896

RESUMO

Cadmium (Cd) is one of the heavy metals that negatively affects the growth of plants. High solubilization in water leads Cd to enter into plants quite easily, thus decreasing seed germination, photosynthesis, and transpiration. It also shows an antagonistic effect with many of the plants' nutrients like Mn, Ca, K, Mg and Fe. Nowadays, inoculation of plants with ACC deaminase (ACCD) rhizobacteria to mitigate Cd's adverse effects has drawn the attention of environmental microbiologists. The rhizobacteria secrete organic compounds that can immobilize Cd in soil. Therefore, this study was accomplished to investigate the effect of ACCD plant growth promoting rhizobacteria (PGPR) on the bitter gourd under Cd stress. There were six treatments consisting of two ACCD PGPR (Stenotrophomonas maltophilia and Agrobacterium fabrum) strains and inorganic fertilizers at two levels of Cd, i.e., 2 (Cd2) and 5 mg kg-1 soil (Cd5). The results showed A. fabrum with the recommended NPK fertilizer (RNPKF) significantly increased the vine length (48 and 55%), fresh weight (24 and 22%), and contents of chlorophyll a (79 and 50%), chlorophyll b (30 and 33%) and total chlorophyll (61 and 36%), over control at the two Cd levels i.e., Cd2 and Cd5, respectively. In conclusion, the recommended NPK fertilizer + A. fabrum combination is a very effective treatment with which to immobilize Cd in soil for the improvement of bitter gourd growth.

9.
Curr Drug Targets ; 2020 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-33050858

RESUMO

Nature has provided prodigious reservoirs of pharmacologically active compounds for drug development since times. Physcion and physcion 8-O-ß-D-glucopyranoside (PG) are bioactive natural anthraquinones which exert anti-inflammatory and anti-cancer properties with minimum or no adverse effects. Moreover, physcion also exhibits anti-microbial and hepatoprotective properties while PG is known to have anti-sepsis as well as ameliorative activities against dementia. This review aims to highlight the natural sources and anti-cancer activities of physcion and PG along with asso-ciated mechanisms of actions. On the basis of the literature, physcion and PG regulate multitudinous cell signaling path-ways through the modulation of various regulators of cell cycle, protein kinases, microRNAs, transcriptional factors, and apoptosis linked proteins resulting in the effective killing of cancerous cells in vitro as well as in vivo. Both compounds effectively suppress metastasis, furthermore, physcion acts as inhibitor of 6PGD and also play an important role in chemosensitization. This review article suggests that physcion and PG are potent anti-cancer drug candidates but further investigations on their mechanism of action and pre-clinical trials are mandatory in order to comprehend the full potential of these natural cancer killers in anti-cancer remedies.

10.
Food Sci Nutr ; 8(8): 4009-4016, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32884682

RESUMO

Peripheral nerve injury (PNI) is an incapacitating situation and has no effective therapy until now. We examined the possible role of crude leaves of Moringa oleifera Lam. at 200 mg/kg body weight in accelerating the functional regain in the sciatic nerve lesion induced mouse model (Adult male albino mice (BALB/c). Motor functions were evaluated by using the sciatic functional index, muscle mass, and muscle grip strength measurement, whereas the sensory functions were evaluated by using the hot plate test. Blood glucose levels and blood cell composition were also analyzed. We found that the Moringa oleifera crude leaves endorse the sensory and motor functions reclamation following the PNI with a statistically significant difference (p < .05). It also revitalizes the gastrocnemius muscle by mass restoration with glycemic management perspective. Conclusively, the crude powder of Moringa oleifera leaves exhibited a function restoration boosting property and further detailed studies for its application as a therapeutic agent are strongly recommended.

11.
Polymers (Basel) ; 12(9)2020 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-32971747

RESUMO

The scope of additive manufacturing, particularly fused deposition modelling (FDM), can indeed be explored with the fabrication of multi-material composite laminates using this technology. Laminar composite structures made up of two distinct materials, namely acrylonitrile butadiene styrene (ABS) and carbon fiber reinforced polylactic acid (CF-PLA), were produced using the FDM process. The current study analyzes the effect of various printing parameters on the interfacial bond strength (IFBS) of the ABS/CF-PLA laminar composite by employing response surface methodology. The physical examination of the tested specimens revealed two failure modes, where failure mode 1 possessed high IFBS owing to the phenomenon of material patch transfer. Contrarily, failure mode 2 yielded low IFBS, while no patch transfer was observed. The analysis of variance (ANOVA) revealed that printing parameters were highly interactive in nature. After extensive experimentation, it was revealed that good quality of IFBS is attributed to the medium range of printing speed, high infill density, and low layer height. At the same time, a maximum IFBS of 20.5 MPa was achieved. The study presented an empirical relation between printing parameters and IFBS that can help in forecasting IFBS at any given printing parameters. Finally, the optimized printing conditions were also determined with the aim to maximize IFBS.

12.
Food Chem Toxicol ; 145: 111642, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32783998

RESUMO

Natural products, being richly endowed with curative powers, have become spotlight for biomedical and pharmaceutical research to develop novel therapeutics during recent years. Ginkgetin (GK), a natural non-toxic biflavone, has been shown to exhibit anti-cancer, anti-inflammatory, anti-microbial, anti-adipogenic, and neuroprotective activities. GK combats cancer progression by arresting cell cycle, inducing apoptosis, stimulating autophagy, and targeting many deregulated signaling pathways such as JAK/STAT and MAPKs. GKhalts inflammation mediators like interleukins, iNOS, COX-2, PGE2, NF-κB, and acts as an inhibitor of PLA2. GK shows strong neuroprotection against oxidative stress-promoted cell death, inhibits cerebral micro-hemorrhage, decreases neurologic deficits, and halts apoptosis of neurons. GK also acts as anti-fungal, anti-viral, anti-bacterial, leishmanicidal and anti-plasmodial agent. GK shows substantial preventive or therapeutic effects in in vivo models of many diseases including atherosclerosis, cancer, neurodegenerative, hepatic, influenza, and inflammatory diseases. Based on various computational, in vitro and in vivo evidences, this article demonstrates the potential of ginkgetin for development of therapeutics against various diseases. Although GK has been systematically studied from pharmacological point of view, a vast field of pharmacokinetics, pre-clinical and clinical studies is still open for the researchers to fully validate its potential for the treatment of various diseases.

13.
Environ Res ; 190: 110017, 2020 11.
Artigo em Inglês | MEDLINE | ID: mdl-32768475

RESUMO

Nature as an infinite treasure of chemotypes and pharmacophores will continue to play an imperative role in the drug discovery. Natural products (NPs) such as plant and fungal metabolites have emerged as leads in drug discovery during recent years due to their efficacy, safety and selectivity. The current review summarizes natural sources as well as pharmacological potential of hispolon which is a major constituent of traditional medicinal mushroom Phellinus linteus. The study aims to update the scientific community about recent developments of hispolon in the arena of natural drugs by providing insights into its present status in therapeutic pursuits. Hispolon, a polyphenol has been reported to possess anticancer, antidiabetic, antioxidant, antiviral and anti-inflammatory activities. It fights against cancer via induction of apoptosis, halting cell cycle and inhibition of metastasis by targeting various cellular signaling pathways including PI3K/Akt, MAPK and NF-κB. The current review proposes that hispolon provides a novel opportunity for pharmacological applications and its styrylpyrone carbon skeleton might serve as an attractive scaffold for drug development. However, future researches are recommended to assess bioavailability, toxicological limits, pharmacokinetic and pharmacodynamic profiles of hispolon, in order to establish its potential as a potent multi-targeted drug in the near future.

14.
Polymers (Basel) ; 12(9)2020 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-32825212

RESUMO

The innovative Incremental Sheet Forming (ISF) process affects the post-forming properties of thermoplastic polymers. However, the effects of degree of plastic strain, and the orientation and size of specimen on the mechanical properties are still unknown. In the present study, therefore, the ISF process is performed on a polymer sheet by varying the plastic strain ranging from 6% to 108%. The corresponding effects on the properties and associated polymer structure are quantified by conducting a variety of mechanical and structural tests. The results reveal that the post-ISF tensile properties like yield stress, ultimate stress, drawing stress, elastic modulus and elongation decrease from 26.6 to 10 MPa, 30.5 to 15.4 MPa, 18.9 to 9.9 MPa, 916 to 300 MPa and 1107% to 457%, respectively, as the strain increases in the investigated range. The value of post-ISF relaxation properties, contrary to the tensile properties, increases with increasing strain up to 62%. Particularly, reductions in stress, strain and modulus increase from 41% to 202%, 37% to 51%, and 41% to 202%. As regard the orientation effect, the sheet in the feed direction shows greater strength than the transverse direction (up to 142% in yield stress and 72% in ultimate stress). Moreover, the smaller sample offers greater strength than the larger one (up to 158% in yield stress and 109% in ultimate stress). The analysis of the post-ISF tensile properties and structural results lead us to conclude that the drop in the tensile properties due to increasing strain occurs due to corresponding increase in the voids area fraction (1.25% to 31%) and a reduction in the crystallinity (38% to 31%).

15.
Cureus ; 12(6): e8481, 2020 Jun 06.
Artigo em Inglês | MEDLINE | ID: mdl-32642385

RESUMO

INTRODUCTION: This study was conducted to evaluate the efficacy of proximal fibular osteotomy (PFO) in terms of pain relief, and improvement in function in medial compartment osteoarthritis (OA) of the knee joint. MATERIALS AND METHODS: This case series study was conducted at the Shaheed Mohtarma Benazir Bhutto Medical College Lyari and United Medical and Dental College of Karachi. Patients with medial compartment knee joint OA were included in the study and patients with bicompartmental or tricompartmental OA, inflammatory joint disease, valgus knee deformity, morbid obesity, or any infectious pathology involving the knee joint were excluded from the study. The medial and lateral joint spaces were measured and recorded preoperatively and postoperatively. Pre- and postoperative values of visual analog scale (VAS) results and the Oxford knee score were recorded. A PFO was performed after getting informed written consent. RESULTS:  A total of 60 patients were enrolled in the study; 16 (26.7%) were men, and 44 (73.3%) were women. The mean age of patients was 51.8 ± 4.1 years. The mean preoperative medial joint space measurement on standard anteroposterior radiograph was 1.45 ± 0.28 mm. The mean preoperative lateral joint space was 8.86 ± 1.27 mm. The recorded mean preoperative Oxford knee score was 20.82 ± 1.97 mm. Recorded levels of mean postoperative medial joint space improved to 4.63 ± 0.668 mm, and mean postoperative lateral joint space was 4.72 ± 0.79 mm. Mean recorded levels of VAS for pain postoperatively were 2.32 ± 0.792, which improved significantly from 7.90 ± 0.79. CONCLUSIONS:  PFO is a good surgical technique for pain relief and functional improvement in patients suffering from medial compartment OA.

16.
Food Chem Toxicol ; 143: 111570, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32640345

RESUMO

Nerium oleander, a member of family Apocynaceae, is commonly known as Kaner in various countries of Asia and Mediterranean region. This plant has been renowned to possess significant therapeutic potential due to its various bioactive compounds which have been isolated from this plant e.g., cardiac glycosides, oleandrin, α-tocopherol, digitoxingenin, urosolic acid, quercetin, odorosides, and adigoside. Oleandrin, a saponin glycoside is one of the most potent and pharmacologically active phytochemicals of N. oleander. Its remarkable pharmacotherapeutic potential have been interpreted as anticancer, anti-inflammatory, anti-HIV, neuroprotective, antimicrobial and antioxidant. This particular bioactive entity is known to target the multiple deregulated signaling cascades of cancer such as NF-κB, MAPK, and PI3K/Akt. The main focus of the current study is to comprehend the action mechanisms of oleandrin against various pathological conditions. The current review is a comprehensive summary to facilitate the researchers to understand the pharmacological position of the oleandrin in the arena of drug discovery, representing this compound as a new drug candidate for further researches. Moreover, in vivo and in silico based studies are required to explore the mechanistic approaches regarding the pharmacokinetics and biosafety profiling of this compound to completely track its candidature status in natural drug discovery.

17.
Curr Drug Metab ; 2020 Jul 28.
Artigo em Inglês | MEDLINE | ID: mdl-32723267

RESUMO

Natural products, an infinite reserve of bioactive molecules, will continue to serve humans as an important source of therapeutic agents. Germacrone is a bioactive natural compound found in the traditional medicinal plants of family Zingi-beraceae.This multifaceted chemical entity has become a point of focus during recent years due to its numerous pharmaco-logical applications e.g., anticancer, anti-inflammatory, antiviral, antioxidant, anti-adipogenic, anti-androgenic, antimicrobi-al, insecticidal, and neuroprotective properties. Germacrone is an effective inducer of cell cycle arrest and apoptosis in vari-ous cancers (breast, brain, liver, skin, prostate, gastric, and esophageal)viamodulation of different cell signaling molecules and pathways involved in cancer proliferation. This is the first report highlighting the wide spectrum of pharmacological ac-tivities exhibited by germacrone. The reported data collected from various shreds of evidences recommend that this multi-faceted compound could serve as a potential drug candidate in the near future.

18.
Mini Rev Med Chem ; 2020 Jul 09.
Artigo em Inglês | MEDLINE | ID: mdl-32646359

RESUMO

Nature has always proved to be a significant reservoir of bioactive scaffolds which have been used for the discovery of drugs since times. Medicinal plants continue to be a solid niche for biologically active and therapeutically effective chemical entities, opening up new avenues for the successful treatment of several human diseases. Contribution of plant-derived compounds either in their original or semi-synthetic derivative forms extends far back in time in drug discovery. This review aims to focus on the sources, biological, and pharmacological profile of a pharmacologically active plant-derived coumarin, osthole, which is an important component of numerous remedial plants such as Cnidium monnieri. Several studies have revealed that osthole possess pharmacological properties such as anticancer, antioxidant, antihyperglycemic, neuroprotective, and antiplatelet. Osthole has been reported to regulate various signaling pathways which in turn modulate several apoptosis related proteins, cell cycle regulators, protein kinases, transcriptional factors, cytokines, and growth receptors affiliated with inflammation, proliferation and several other ailments. Osthole is known to halt proliferation and metastasis of cancerous cells by arresting the cell cycle and inducing apoptosis. The data in this review paper supports the pharmacological potential of osthole but further experimentation, biosafety profiling and synergistic effects of this compound need to be focused by the researchers to understand the full spectrum of pharmacological potential of this therapeutically potent compound.

19.
BMC Complement Med Ther ; 20(1): 181, 2020 Jun 11.
Artigo em Inglês | MEDLINE | ID: mdl-32527244

RESUMO

BACKGROUND: Peripheral nerve injury is a debilitating condition that may lead to partial or complete motor, sensory and autonomic function loss and lacks effective therapy until date. Therefore, it is quite imperative to explore impending remedies for rapid and accurate functional retrieval following such conditions. Natural product-based intervention can prove effective to facilitate the process of functions regain. METHODS: Here, we investigated the effect of processed Strychnos nux-vomica seeds at a dose of 250 mg/kg body weight in a mouse model of induced Sciatic nerve lesion in promoting the recovery of the functions. A compression injury was induced in the Sciatic nerve of the right leg in the mice. Sensory function recovery was evaluated by hot-plate and formalin tests, whereas the motor function retrieval was assessed by measuring muscle grip strength, sciatic functional index, and muscle mass restoration. Oxidative stress and blood cell count were measured by biochemistry and haematological analyses. RESULTS: This study indicates that Strychnos nux-vomica seeds enhance the rate of recovery of both sensory and motor functions. It helps restore the muscle mass, attenuates total oxidant status and enhances the total anti-oxidant capacity of the biological system. Moreover, the treated animals manifested an enhanced glucose tolerance aptitude and augmented granulocyte and platelet counts. Improved oxidant control, enhanced glucose sensitivity and amended granulocyte and platelet counts are likely to contribute to the advantageous effects of Strychnos nux-vomica, and warrant further in-depth studies for deciphering possible mechanisms and identification of active constituent(s) responsible for these effects. CONCLUSION: Strychnos nux-vomica seed offers functional recovery promoting effects following a mechanical injury to the Sciatic nerve and the possible reasons behind this effect can be reduced oxidative stress and improved glycaemic control. Further and detailed investigations can unravel this mystery.


Assuntos
Lesões por Esmagamento/tratamento farmacológico , Estresse Oxidativo/efeitos dos fármacos , Traumatismos dos Nervos Periféricos/tratamento farmacológico , Preparações de Plantas/uso terapêutico , Neuropatia Ciática/tratamento farmacológico , Strychnos nux-vomica/química , Animais , Modelos Animais de Doenças , Masculino , Camundongos , Recuperação de Função Fisiológica , Sementes/química
20.
Sci Rep ; 10(1): 8958, 2020 06 02.
Artigo em Inglês | MEDLINE | ID: mdl-32488033

RESUMO

Whitefly infestation of cotton crop imparts enormous damage to cotton yield by severely affecting plant health, vigour and transmitting Cotton Leaf Curl Virus (CLCuV). Genetic modification of cotton helps to overcome both the direct whitefly infestation as well as CLCuV based cotton yield losses. We have constitutively overexpressed asparaginase (ZmASN) gene in Gossypium hirsutum to overcome the cotton yield losses imparted by whitefly infestation. We achieved 2.54% transformation efficiency in CIM-482 by Agrobacterium-mediated shoot apex transformation method. The relative qRT-PCR revealed 40-fold higher transcripts of asparaginase in transgenic cotton line vs. non-transgenic cotton lines. Metabolic analysis showed higher contents of aspartic acid and glutamic acid in seeds and phloem sap of the transgenic cotton lines. Phenotypically, the transgenic cotton lines showed vigorous growth and height, greater number of bolls, and yield. Among six representative transgenic cotton lines, line 14 had higher photosynthetic rate, stomatal conductance, smooth fiber surface, increased fiber convolutions (SEM analysis) and 95% whitefly mortality as compared to non-transgenic cotton line. The gene integration analysis by fluorescence in situ hybridization showed single copy gene integration at chromosome number 1. Collectively, asparaginase gene demonstrated potential to control whitefly infestation, post-infestation damages and improve cotton plant health and yield: a pre-requisite for farmer's community.

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