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1.
Int J Paediatr Dent ; 2021 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-34608702

RESUMO

BACKGROUND: Though prefabricated zirconia crowns (PZCs) have different properties from stainless steel crowns and different tooth preparation principles for optimal crown adaptation, little is known about how clinicians prepare teeth for PZCs. AIM: To investigate prepared tooth forms for PZCs and identify the shared patterns of tooth preparation. DESIGN: Twenty participants prepared primary maxillary first molars and mandibular second molars for PZCs. A model with ideally prepared tooth was digitally generated as a reference. The prepared teeth were digitally scanned and superimposed to the reference. A three-dimensional analysis software (Gom Inspect 2018 software) was used to evaluate the discrepancy between ideal and prepared surface for overall surface and subdivided area. RESULTS: Half of the surfaces received excessive reduction, and 24 % showed error within 0.2mm (p < 0.001) in maxilla. 41.6 % of surfaces showed error within 0.2mm and 20 % were excessively prepared (p = 0.002) in mandible. The highest discrepancies were at the mesiopalatal line angles (maxillary) and the distobuccal line angles (mandibular). There were significant discrepancies on the occlusal surfaces, especially the cusps. CONCLUSIONS: Tooth preparations for PZCs showed wide variations related to depth among the participants.

2.
Dent Mater J ; 2021 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-34471038

RESUMO

This study aimed to determine functionality and mechanical properties of dental splints. Five splints were tested: a control splint Filtek Z350XT (ZF), two splints with G-Fix (GF) and Light Fix (LF), and two wire-composite splints with ZF (ZW) and Ortho Connect Flow (CW). Periotest values, elastic modulus, flexural, compressive, and diametral tensile strength were measured. ZW and CW showed no significant differences in flexibility in labial or occlusal pressure of upper central incisors. LF, GF, and ZF did not differ in labial or palatal pressure. ZW and CW had higher flexibility than LF, GF, and ZF. LF had the lowest flexural and compressive strength but the highest diametral tensile strength. This study showed that ZW and CW might be options for flexible splints, whereas LF and GF would be options for rigid splints. Additionally, CW and LF/GF might be simpler alternatives to ZW and ZF, respectively.

3.
J Clin Pediatr Dent ; 45(3): 186-192, 2021 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-34192754

RESUMO

OBJECTIVE: To compare the shear bond strength (SBS) after thermocycling of four universal adhesives applied in self-etch mode on dentin and etch-and-rinse mode on enamel. STUDY DESIGN: Flat 144 buccal or lingual dentin and enamel surfaces from 72 non-carious primary molars were prepared. Samples were segregated into 12 groups (n=12): Adper Single Bond 2 etch-and-rinse (SB_T) and Clearfil SE Bond self-etch (SE_S) applied to enamel and dentin served as controls. Scotch Bond Universal Adhesive (SBU), Clearfil S3 Bond Universal Adhesive (SEU), Tetric N-Bond Universal Adhesive (TEN), and All Bond Universal (BIS) were applied in etch-and-rinse mode to enamel and in self-etch mode to dentin. They were thermocycled for 5000 cycles. SBS testing and the evaluation of fracture mode were performed. RESULTS: SB_T showed statistically higher SBS than other adhesive groups using etch-and-rinse mode on enamel. SE_S and BIS had statistically higher SBS than other adhesive groups using self-etch mode on dentin. Mixed failure was the most common failure mode in each group. CONCLUSION: The universal adhesives did not show higher SBS than SB_T when using etch-and-rinse on enamel. All universal adhesives showed higher SBS than SB_T and had SBS similar to SE_S, except SBU when using self-etch mode on dentin.


Assuntos
Colagem Dentária , Adesivos Dentinários , Esmalte Dentário , Dentina , Humanos , Teste de Materiais , Cimentos de Resina , Resistência ao Cisalhamento , Dente Decíduo
4.
Dent Mater J ; 40(3): 727-735, 2021 May 29.
Artigo em Inglês | MEDLINE | ID: mdl-33563847

RESUMO

This study aimed to compare the change of surface roughness (Ra) and gloss units (GU) of five dental composites (Filtek Z250, Filtek Z350XT, Metafil CX, Ceram X one, and Venus Diamond) polished with three systems (Sof-Lex XT, Enhance/Pogo, and Sof-Lex Diamond) before/after simulated brushings and to determine the amount of time required to achieve maximum gloss. Ninety rectangular specimens (n=18 per composite) were prepared. Six specimens of each composite were assigned to one of the polishing systems. The Ra and GU of each specimen were measured after each polishing step. Five polished specimens per composite were brushed with a toothbrush machine, and the Ra and GU values were determined. Filtek Z350XT exhibited the most stable and lowest Ra during the brushing cycles regardless of polishing system. When using the Sof-Lex Diamond and Enhance/Pogo systems, the highest gloss and the smoothest surfaces were achieved after polishing and brushing.


Assuntos
Resinas Compostas , Polimento Dentário , Diamante , Teste de Materiais , Propriedades de Superfície , Escovação Dentária
5.
Arch Oral Biol ; 117: 104815, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32585446

RESUMO

AIM: To investigate the chemical and mechanical properties of teeth affected by a 1-bp deletion (c.2688delT) in the DSPP gene. METHODS AND MATERIALS: Maxillary first premolars were extracted from the affected individual at age 9 years due to the orthodontic reason for crowding. A sample was imbedded in epoxy resin and sectioned buccolingually, after micro-computerized tomography (µCT) images were taken. Scanning Electron Microscopy (SEM), Energy Dispersive Spectrometry (EDS) and Vickers microhardness testing were also performed. RESULTS: µCT reconstruction and analysis showed an irregularly obliterated pulp chamber and an extremely small pulpal volume in the DGI-II sample. The mineral density and microhardness scores were smaller in the dentin of the DGI-II sample compared to the wild-type. Mg content was lower in the dentin of the DGI-II sample compared to the wild-type. CONCLUSION: This study shows that dentin affected by a 1-bp deletion in DSPP has a reduced mineral density, diminished microhardness and reduced Mg content.


Assuntos
Dentinogênese Imperfeita , Proteínas da Matriz Extracelular/genética , Fosfoproteínas/genética , Sialoglicoproteínas/genética , Criança , Dentina/patologia , Dentinogênese Imperfeita/diagnóstico por imagem , Dentinogênese Imperfeita/genética , Humanos , Mutação , Linhagem , Deleção de Sequência
6.
Sci Rep ; 10(1): 2354, 2020 02 11.
Artigo em Inglês | MEDLINE | ID: mdl-32047246

RESUMO

Nitrous oxide, the least potent inhalation anesthetic, is widely used for conscious sedation. Recently, it has been reported that the occurrence of anesthetic-induced loss of consciousness decreases the interconnection between brain regions, resulting in brain network changes. However, few studies have investigated these changes in conscious sedation using nitrous oxide. Therefore, the present study aimed to use graph theory to analyze changes in brain networks during nitrous oxide sedation. Participants were 20 healthy volunteers (10 men and 10 women, 20-40 years old) with no history of systemic disease. We acquired electroencephalogram (EEG) recordings of 32 channels during baseline, nitrous oxide inhalation sedation, and recovery. EEG epochs from the baseline and the sedation state (50% nitrous oxide) were extracted and analyzed with the network connection parameters of graph theory. Analysis of 1/f dynamics, revealed a steeper slope while in the sedation state than during the baseline. Network connectivity parameters showed significant differences between the baseline and sedation state, in delta, alpha1, alpha2, and beta2 frequency bands. The most pronounced differences in functional distance during nitrous oxide sedation were observed in the alpha1 and alpha2 frequency bands. Change in 1/f dynamics indicates that changes in brain network systems occur during nitrous oxide administration. Changes in network parameters imply that nitrous oxide interferes with the efficiency of information integration in the frequency bands important for cognitive processes and attention tasks. Alteration of brain network during nitrous oxide administration may be associated to the sedative mechanism of nitrous oxide.


Assuntos
Encéfalo/fisiologia , Conectoma , Sedação Consciente/métodos , Óxido Nítrico/farmacologia , Adulto , Encéfalo/efeitos dos fármacos , Eletroencefalografia/métodos , Feminino , Humanos , Masculino
7.
J Clin Pediatr Dent ; 44(1): 55-59, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31995414

RESUMO

Molar root-incisor malformation (MRIM) or molar-incisor malformation (MIM) is a new type of dental anomaly characterized by dysplastic roots of permanent first molars, occasionally second primary molars, and the crowns of maxillary central incisors. MRIM involving permanent first molars and second primary molars is characterized by normal crowns with short, thin, and narrow roots, whereas MRIM involving permanent maxillary central incisors exhibits constrictions of the crown in the cervical area. In the first case, we extracted the affected first permanent molars at the optimal timing to minimize space deficiencies and induce space closure. In addition, composite resin restorations were performed on the anterior central incisors. In the second case, a mandibular lingual arch was used to stabilize the affected teeth in order to mitigate discomfort by reducing rotational biting forces.


Assuntos
Incisivo , Raiz Dentária , Criança , Coroas , Humanos , Dente Molar , Dente Decíduo
8.
Arch Oral Biol ; 111: 104629, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31865033

RESUMO

OBJECTIVE: This study aimed to suggest criteria for the early diagnosis of agenesis of the maxillary second premolars (U5) based on the developmental stages of the maxillary canines (U3), first premolars (U4), and second molars (U7). DESIGN: Overall, 303 control patients and 72 patients with agenesis who met the inclusion criteria were analyzed among 5136 patients aged 5-11 years for whom panoramic radiographs were obtained at Seoul National University Dental Hospital from June 2008 to December 2009. All developmental stages of U3, U4, U5, and U7 in both groups were evaluated and categorized into the stages proposed by Demirjian et al. To confirm the delayed dental development in the agenesis group, the Wilcoxon rank sum test was used. For verifying the tooth with the most similar developmental pattern to U5, the Kendall tau Rank Correlation and Bootstrap methods were used. Moreover, survival analysis and leave-one-out-cross-validation were performed to identify the earliest developmental stages of U3, U4, and U7 at which agenesis of U5 can be confirmed. RESULTS: The developmental stage of U4 in the agenesis group was significantly delayed compared with that in the control group at ages 5-10. All of the stages of U3, U4, and U7 showed high correlation coefficients with U5. CONCLUSION: U5 agenesis can be confirmed with high prediction accuracy when at least two of the three conditions are satisfied: U3, U4, and U7 in Demirjian stages E, D, and C, respectively.


Assuntos
Dente Molar , Anodontia , Dente Pré-Molar , Criança , Pré-Escolar , Humanos , Radiografia Panorâmica
10.
Arch Oral Biol ; 103: 8-11, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31103801

RESUMO

OBJECTIVES: To identify the molecular genetic etiology of the families with non-syndromic multiple missing permanent teeth (oligodontia). MATERIALS AND METHODS: Genomic DNA was isolated and measured, and whole-exome sequencing was performed. The obtained sequencing reads were aligned to the human reference genome and subsequently processed by a series of bioinformatics programs. Finally, short insertions/deletions and single nucleotide variations were annotated with dbSNP build 138. RESULTS: The proband of family 1 was missing 14 permanent teeth, and the mutational analysis revealed compound heterozygousWNT10A mutations (c.364A > T and c.511C > T). Two affected individuals in family 2 were missing 20 and 12 permanent teeth, respectively, and compound heterozygous WNT10A mutations (c.364A > T and c.637G > A) were also identified. CONCLUSIONS: This study reveals compound heterozygousWNT10A missense mutations in two families with non-syndromic oligodontia which will improve the understanding of odontogenesis and the pathogenesis related to WNT10A mutations.


Assuntos
Anodontia , Proteínas Wnt/genética , Anodontia/genética , Análise Mutacional de DNA , Dentição Permanente , Humanos , Mutação , Linhagem , Sequenciamento Completo do Exoma
11.
Int Dent J ; 69(5): 354-360, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30957875

RESUMO

OBJECTIVES: To examine oral health status and access to dental care among marriage-immigrant women (MIW) in South Korea (Korea). MATERIALS AND METHODS: We analysed patients' demographic information and access to dental services through the 'Family-Love Dental Program' and compared them with Korean oral health surveillance data. The 'Family-Love Dental Program' resulted from a partnership among the Korean Ministry of the Gender Equality and Family, Seoul National University Dental Hospital, and Cigna Insurance Company (registered as 'LINA' in South Korea) to increase access to oral health for MIW from 2010 to 2016. RESULTS: Over the programme duration, 1,944 MIW underwent dental examination and 1598 MIW received dental treatment. More than two-thirds of MIW (69.5%) receiving dental services were aged between 20 and 39 years. In 2016, the proportion of untreated dental caries among MIW was 54.8%, which was significantly higher than that of untreated dental caries among Korean women within the same age group (24.9%). Over the programme duration, 501 MIW (25.8%) underwent final restoration, and advanced care was provided at the Seoul National University Dental Hospital. CONCLUSION: MIW have a significantly higher number of dental caries than Korean women within the same age group. Future programmes and policies should consider specific barriers that will promote oral health parity for MIW and their children.


Assuntos
Cárie Dentária , Emigrantes e Imigrantes , Adulto , Criança , Feminino , Humanos , Casamento , Saúde Bucal , República da Coreia , Adulto Jovem
12.
Clin Oral Investig ; 23(3): 1481-1487, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30120606

RESUMO

OBJECTIVE: Amelogenesis imperfecta (AI) is a rare hereditary disorder affecting the quality and quantity of the tooth enamel. The purpose of this study was to identify the genetic etiology of hypoplastic AI families based on the candidate gene approach. MATERIALS AND METHODS: We recruited three Turkish families with hypoplastic AI and performed a candidate gene screening based on the characteristic clinical feature to find the pathogenic genetic etiology. RESULTS: The candidate gene sequencing of the LAMB3 gene for family 1 revealed a heterozygous nonsense mutation in the last exon [c.3431C > A, p.(Ser1144*)]. FAM20A gene sequencing for families 2 and 3 identified a homozygous deletion [c.34_35delCT, p.(Leu12Alafs*67)] and a homozygous deletion-insertion (c.1109 + 3_1109 + 7delinsTGGTC) mutation, respectively. CONCLUSION: The candidate gene approach can be successfully used to identify the genetic etiology of the AI in some cases with characteristic clinical features. CLINICAL RELEVANCE: Identification of the genetic etiology of the AI will help both the family members and dentist understand the nature of the disorder. Characteristic clinical feature can suggest possible genetic causes.


Assuntos
Amelogênese Imperfeita/genética , Moléculas de Adesão Celular/genética , Proteínas do Esmalte Dentário/genética , Códon sem Sentido , Análise Mutacional de DNA , Homozigoto , Humanos , Mutação INDEL , Linhagem , Deleção de Sequência , Turquia
13.
BMC Oral Health ; 18(1): 92, 2018 05 29.
Artigo em Inglês | MEDLINE | ID: mdl-29843690

RESUMO

BACKGROUND: The findings that not only dental caries but also systemic disease can exert a negative effect on oral health-related quality of life (OHRQoL), and that dental treatment can improve OHRQoL have been confirmed in multiple studies. The purpose of this study is to investigate the impact of dental treatment on OHRQoL of Korean pediatric patients and the differences in OHRQoL between patients with and without systemic disease. METHODS: All the primary caregivers of pediatric patients who underwent dental treatments under either general anesthesia or intravenous deep sedation at Seoul National University Dental Hospital completed abbreviated versions of the Child Oral Health Impact Profile (COHIP-14) and Family Impact Scale (FIS-12) surveys on OHRQOL pre- and post-treatment (average: 2.4 ± 1.7 months after dental treatment). This is a case control study with patients divided into two groups according to the presence or absence of systemic disease. RESULTS: Data from 93 pediatric patients (46 male and 47 female, average patient age: 5.0 ± 3.4 years) were analyzed to compare OHRQoL before and after treatment with the Wilcoxon signed-rank test and to calculate the effect size using Cohen's d. All of the patients exhibited an improvement in OHRQoL (COHIP-14: p <  0.001, effect size = 1.0; FIS-12: p <  0.001, effect size = 0.7). Patients with systemic diseases demonstrated lower OHRQoL in both pre- and post-treatment surveys than patients without systemic diseases (Wilcoxon Rank-sum test, both COHIP-14 and FIS-12: p <  0.05). The COHIP-14 appears to have a greater impact on the FIS-12 in patients with systemic disease than those without (explanatory power of 65.3 and 44.6%, respectively). CONCLUSIONS: Based on the primary caregivers' perceptions, dental treatment can improve the OHRQoL in Korean pediatric patients. Systemic disease results in a reduced OHRQoL, and the awareness of patients' oral health appeared to have a greater impact on OHRQoL for family members of patients with a systemic disease. TRIAL REGISTRATION: KCT0002473 (Clinical Research Information Service, Republic of Korea) and 22 Sep 2017, retrospectively registered.


Assuntos
Cárie Dentária/terapia , Saúde Bucal , Qualidade de Vida , Anestesia Geral , Anestesia Intravenosa , Estudos de Casos e Controles , Criança , Pré-Escolar , Comorbidade , Cárie Dentária/complicações , Inquéritos de Saúde Bucal , Feminino , Avós/psicologia , Humanos , Masculino , Pais/psicologia , República da Coreia
14.
Nephron ; 139(2): 189-196, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29439260

RESUMO

BACKGROUND/AIMS: Enamel-renal syndrome is characterized by nephrocalcinosis, enamel defects, gingival hyperplasia and eruption failures. It has been recently identified that recessive mutations in the FAM20A gene result in amelogenesis imperfecta (AI)-gingival fibromatosis. The aim of this research to determine whether AI patients with known -FAM20A mutations also have nephrocalcinosis. METHODS: Complete oral and radiological examinations were performed for all participating family members. Renal examinations were performed using ultrasound. RESULTS: The teeth were evaluated for severe loss, and multiple eruption failures were evident from the clinical and radiological examinations. Unexpected extensive and fast crown resorption was found by radiological examination. Renal ultrasound revealed bilateral nephrocalcinosis in both affected individuals. Recessive FAM20A mutations can cause nephrocalcinosis in addition to the oral phenotype. CONCLUSION: AI patients with similar clinical phenotypes and FAM20A mutations should be examined for nephropathy even if they lack pertinent symptoms. Nephrology referral is warranted for patients who have clinical phenotypes related to AI-gingival fibromatosis even if they are not symptomatic.


Assuntos
Amelogênese Imperfeita/genética , Proteínas do Esmalte Dentário/genética , Mutação , Nefrocalcinose/genética , Feminino , Humanos , Masculino
15.
Arch Oral Biol ; 87: 110-114, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29277045

RESUMO

OBJECTIVE: The aim in this study was to suggest a standard for early diagnosis of agenesis of the mandibular second premolars (L5) by estimating the developmental stages of the mandibular canines (L3), first premolars (L4), and second molars (L7). DESIGN: Among all 5136 pediatric patients aged 5-11 years who received panoramic radiographs between June 2008 and December 2009 at Seoul National University Dental Hospital, 215 control patients and 74 agenesis patients who met inclusion criteria were analyzed. The developmental stages of all L3, L4, L5, and L7 of control and agenesis patients were estimated using the method proposed by Demirjian. To identify the tooth (L3, L4, L7) with the developmental pattern most similar to that of L5, Kendall rank correlation coefficients and Bootstrap method were used. To verify that patients with agenesis of L5 show delayed development, Wilcoxon rank sum test was used. To identify the stages in which to diagnose agenesis of L5, we performed survival analysis. RESULTS: There was a significant correlation between the developmental stages of L3, L4, L7 and L5. The developmental stages of those three teeth in the agenesis group were delayed compared with those in the control group at certain ages. If the developmental stages of at least two of those three teeth reach Demirjian stage D without the calcification of L5, agenesis of L5 can be confirmed. CONCLUSIONS: Agenesis of L5 can be confirmed when two of the three teeth (L3, L4, L7) reach Demirjian stage D.


Assuntos
Anodontia/diagnóstico por imagem , Dente Pré-Molar/anormalidades , Dente Canino/crescimento & desenvolvimento , Dente Molar/crescimento & desenvolvimento , Criança , Pré-Escolar , Feminino , Humanos , Masculino , Mandíbula , Valor Preditivo dos Testes , Radiografia Panorâmica
16.
J Esthet Restor Dent ; 29(5): 362-367, 2017 09.
Artigo em Inglês | MEDLINE | ID: mdl-28628735

RESUMO

OBJECTIVE: The aim of this study was to investigate the effect of shade and opacity on the change in light transmission through different thicknesses of a nano-hybrid composite during curing. MATERIALS AND METHODS: Twelve different shades of Venus Diamond (Heraeus Kulzer) were placed in disk shaped molds with thickness of 1, 2, and 3 mm (n = 3 per group) and cured with an LED light-curing unit. Initial, final and average irradiance, and the total amount of energy passing through the specimen were measured using the MARC Resin Calibrator at every 10s for a total of 40s. The translucency parameter and the contrast ratio were obtained using a chromameter. Results were analyzed with ANOVA/Tukey's test (α = 0.05). RESULTS: All shades and all thicknesses (up to 3 mm) experienced an increase in light transmittance during curing. The majority of the increase occurred during the initial 10s exposure, with significant increase occurring from subsequent exposures only in thicker specimens (i.e., 3 mm). The increase in irradiance at the bottom during curing was dependent on shade, with darker shades and greater depths of material showing less increase. CONCLUSIONS: For one specific resin composite formulation, an increase in translucency occurs as cure progresses, and the increase is enhanced for composites with greater lightness and lower contrast ratio. CLINICAL SIGNIFICANCE: Composites demonstrate increased light transmittance as curing progress, which may improve depth of cure. The thicker composite showed the least increase in light transmission within the same shade. The increase in translucency is enhanced for composites with great lightness and lower contrast ratio.


Assuntos
Resinas Compostas/química , Resinas Compostas/efeitos da radiação , Luzes de Cura Dentária , Cor , Materiais Dentários/química , Teste de Materiais , Propriedades de Superfície
17.
Front Physiol ; 8: 229, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28473773

RESUMO

Amelogenesis imperfecta is a group of rare inherited disorders that affect tooth enamel formation, quantitatively and/or qualitatively. The aim of this study was to identify the genetic etiologies of two families presenting with hypomaturation amelogenesis imperfecta. DNA was isolated from peripheral blood samples obtained from participating family members. Whole exome sequencing was performed using DNA samples from the two probands. Sequencing data was aligned to the NCBI human reference genome (NCBI build 37.2, hg19) and sequence variations were annotated with the dbSNP build 138. Mutations in MMP20 were identified in both probands. A homozygous missense mutation (c.678T>A; p.His226Gln) was identified in the consanguineous Family 1. Compound heterozygous MMP20 mutations (c.540T>A, p.Tyr180* and c.389C>T, p.Thr130Ile) were identified in the non-consanguineous Family 2. Affected persons in Family 1 showed hypomaturation AI with dark brown discoloration, which is similar to the clinical phenotype in a previous report with the same mutation. However, the dentition of the Family 2 proband exhibited slight yellowish discoloration with reduced transparency. Functional analysis showed that the p.Thr130Ile mutant protein had reduced activity of MMP20, while there was no functional MMP20 in the Family 1 proband. These results expand the mutational spectrum of the MMP20 and broaden our understanding of genotype-phenotype correlations in amelogenesis imperfecta.

18.
Angle Orthod ; 87(4): 590-597, 2017 07.
Artigo em Inglês | MEDLINE | ID: mdl-28156127

RESUMO

OBJECTIVE: To examine whether there is a relationship between maxillary canine impaction and the morphologic characteristics of the maxillary dentition, especially the root of the lateral incisor. MATERIALS AND METHODS: In this study, we selected only patients with unilateral maxillary canine impaction to compare the morphologic characteristics of the dentition on the impaction side and the clinically normal eruption side. The sample size was decided to be 40 based on the pilot study. To minimize bias depending on sex and location of the maxillary canine impaction, we selected equal numbers (20) of boys and girls, and equal cases (20) of buccal impaction and palatal impaction. Under the aforementioned conditions, the mean age was 13.5 ± 2.3 years. The multislice spiral computed tomography images of these 40 subjects were converted into three-dimensional (3D) reconstructed images using the OnDemand 3D program (Cybermed Co, Seoul, Korea). Then we measured the morphologic characteristics of the individual teeth on the obtained 3D teeth images. RESULTS: Length and volume of the maxillary lateral incisor's roots were significantly smaller on the impaction side compared with the normal eruption side (P = 0.001 and P = 0.006, respectively). The width and volume of the canine's crown were significantly greater on the impaction side compared with the normal eruption side (P = 0.020 and P < .0001, respectively). CONCLUSION: These results might help to prove the hypothesis that the smaller-sized lateral incisor roots and greater-sized canine crowns are the influential etiologic factors in maxillary canine impaction.


Assuntos
Dente Canino/diagnóstico por imagem , Incisivo/diagnóstico por imagem , Coroa do Dente/diagnóstico por imagem , Raiz Dentária/diagnóstico por imagem , Dente Impactado/diagnóstico por imagem , Adolescente , Dente Canino/anatomia & histologia , Feminino , Humanos , Imageamento Tridimensional , Incisivo/anatomia & histologia , Masculino , Maxila , Tomografia Computadorizada Multidetectores , Tomografia Computadorizada Espiral , Coroa do Dente/anatomia & histologia , Raiz Dentária/anatomia & histologia
19.
Arch Oral Biol ; 76: 61-65, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-28130977

RESUMO

OBJECTIVES: Amelogenesis imperfecta (AI) is a hereditary genetic defect affecting tooth enamel. AI is heterogeneous in clinical phenotype as well as in genetic etiology. To date, more than 10 genes have been associated with the etiology of AI. Amelogenin is the most abundant enamel matrix protein, most of which is encoded by the amelogenin gene in the X-chromosome (AMELX). More than 16 alternative splicing transcripts have been identified in the murine Amelx gene. The purpose of this study was to identify the genetic cause of an AI family. MATERIALS AND METHODS: We recruited a family with hypoplastic AI and performed mutational analysis on the candidate gene based on the clinical phenotype. RESULTS: Mutational analysis revealed a missense mutation in exon 6 (NM_182680.1; c.242C > T), which changes a sequence in a highly conserved amino acid (NP_872621.1; p.Pro81Leu). Furthermore, a splicing assay using a minigene displayed that the mutation changed the mRNA splicing repertory. CONCLUSIONS: In this study, we identified a novel AMELX missense mutation causing hypoplastic AI, and this mutation also resulted in altered mRNA splicing. These results will not only expand the mutation spectrum causing AI but also broaden our understanding of the biological mechanism of enamel formation.


Assuntos
Amelogênese Imperfeita/genética , Amelogenina/genética , Mutação de Sentido Incorreto , Amelogênese Imperfeita/diagnóstico por imagem , Criança , Análise Mutacional de DNA , Humanos , Masculino , Linhagem , Fenótipo , Reação em Cadeia da Polimerase , Radiografia Panorâmica , República da Coreia
20.
Clin Oral Investig ; 21(1): 167-172, 2017 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-26955834

RESUMO

OBJECTIVE: Aplasia of lacrimal and salivary glands (ALSG) is a rare autosomal dominant inherited disease, characterized by aplasia, atresia, or hypoplasia of the lacrimal and salivary systems with variable expressivity. The purpose of this study was to identify genetic etiology of an ALSG family. MATERIALS AND METHODS: We recruited a Turkish family with ALSG and performed a mutational analysis, based on the candidate gene approach, to clarify the molecular genetic etiology. RESULTS: The candidate gene sequencing of the FGF10 gene identified a novel heterozygous nonsense mutation (c.237G > A, p.Trp79*) in the exon 1. CONCLUSION: The identified novel mutation would result in a haploinsufficiency of the FGF10, because of nonsense-mediated mRNA decay caused by a premature stop codon. This report further confirms that ALSG is caused by the haploinsufficiency of functional FGF10. CLINICAL RELEVANCE: Identification of the genetic etiology of the ALSG will help both the family members and dentist understand the nature of the disorder. Therefore, it will positively motivate oral health care to avoid further destruction of the tooth due to the lack of salivary production.


Assuntos
Códon sem Sentido , Fator 10 de Crescimento de Fibroblastos/genética , Aparelho Lacrimal/anormalidades , Glândulas Salivares/anormalidades , Adulto , Pré-Escolar , Éxons , Feminino , Humanos , Lactente , Aparelho Lacrimal/diagnóstico por imagem , Masculino , Dados de Sequência Molecular , Linhagem , Reação em Cadeia da Polimerase , Glândulas Salivares/diagnóstico por imagem , Turquia
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