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1.
J Asthma Allergy ; 16: 107-121, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36714048

RESUMO

Background: Severe bronchial asthma (BA) affects 5-10% of children, which imposes socioeconomic burden. Therefore, it is crucial to identify biomarkers for risk stratification in children with BA. T regulatory cells (Tregs) play a balancing role in allergic response regulation. We aimed to investigate the relationship between Treg, miR-210-3p, and miR-146a-5p in relation to asthma phenotypes in search of novel biomarkers of disease severity. Methods: This study included 50 children with BA classified into Group 1 (n = 25) children with mild to moderate asthma and Group 2 (n = 25) children with severe asthma. In addition to 26 control subjects. Flow cytometry was used to detect Tregs. Plasma miR-210-3p and miR-146a levels were determined using quantitative real-time PCR. Patients' FEV1 (Forced Expiratory Volume in the first second) was measured. Results: miR-210-3p level correlated negatively with Treg frequency (r = -0.828, P < 0.001) and FEV1 (r = -0.621, P < 0.001). The level of miR-146a-5p positively correlated positively with Treg% (r = 0.303, P = 0.032). ROC curve analysis revealed that miR-210-3p was the most sensitive biomarker of severity, with the area under curve (AUC) = 0.923, 96% sensitivity, and 60% specificity. According to multivariate analysis, miR-210-3p is an independent risk factor for BA severity [OR =3.119, P = 0.030], while miR-146a-5p is a protective factor [OR =0.811, P = 0.049]. Conclusion: Treg frequency is linked to FEV1, miR-146a-5p and miR-210-3p in childhood BA. Upregulation of miR-210-3p is a sensitive biomarker and an independent risk factor for BA severity in Egyptian children.

2.
J Parasit Dis ; 46(4): 1011-1020, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36457780

RESUMO

Cryptosporidium has been identified as one of the prevalent opportunistic parasites that cause diarrhea, which may be persistent and fatal. Current chemotherapeutic agents, including nitazoxanide (NTZ), are frequently associated with therapeutic failure, and their roles in the induction of apoptosis in cryptosporidiosis remain to be a topic of debate. Thus, this study aimed to assess the apoptotic changes in cryptosporidiosis in immunocompetent (IC) and immunosuppressed (IS) mice after treatment with silver nanoparticles (AgNPs) and NTZ either alone or after loading. In total, 120 laboratory-bred Swiss albino mice were divided into two groups. Group A included IC mice, while Group B included IS mice. Both groups were divided into six subgroups: noninfected nontreated, infected nontreated, infected AgNP-treated, infected NTZ-treated, infected AgNP-loaded NTZ (full-dose)-treated, and infected AgNP-loaded NTZ (half-dose)-treated. The assessment was achieved through parasitological, histopathological, and apoptotic marker expression evaluation. AgNP-loaded NTZ (different doses) treatment showed the highest oocyst shedding reduction and remarkable improvement in histopathological changes, followed by individual treatment with NTZ and then AgNPs in IC and IS mice. Results of apoptotic marker expression revealed that AgNP-loaded NTZ treatment exhibited a promising role in regulating apoptotic changes in cryptosporidiosis through the expression of the lowest levels of cytochrome C and caspase-3 in IC and IS mice at the end of the experiment. Therefore, AgNP-loaded NTZ can be a potential therapeutic agent against cryptosporidiosis for IC and IS mice.

3.
Egypt J Immunol ; 29(1): 19-28, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-35171544

RESUMO

This study intended to explore the relationship between the +869T/C polymorphism of the transforming growth factor-ß1 (TGF-ß1) gene and rheumatoid arthritis (RA) predisposition and activity in Egyptian patients. The study involved 30 patients suffering from RA and 30 apparently healthy participants as the control group. The +869T/C polymorphism of the TGF-ß1 gene was determined by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) process. The TGF-ß1 + 869 CT genotype and CT+TT genotypes in RA patients showed a significant increase than the control group (OR=3.782 and 3.824, CI=1.046-13.680 and 1.150-12.713, P=0.043 and 0.029, respectively). T allele showed a significant increase in patients than in controls (OR= 2.104, CI 1.015- 4.361, P = 0.046). The TGF-ß1 +869 CT+TT genotypes were accompanied by higher DAS-28 scores which express higher disease activity, and increased levels of RF, Anti-CCP, ESR, and CRP. In conclusion, the TGF-ß1 +869T/C gene polymorphism may be accompanied by an increased predisposition to RA and with its severity in Egyptian RA patients.


Assuntos
Artrite Reumatoide , Predisposição Genética para Doença , Fator de Crescimento Transformador beta1 , Artrite Reumatoide/genética , Egito , Frequência do Gene , Genótipo , Humanos , Polimorfismo de Nucleotídeo Único , Fator de Crescimento Transformador beta1/genética
4.
Tissue Barriers ; 10(3): 1994823, 2022 07 03.
Artigo em Inglês | MEDLINE | ID: mdl-34689723

RESUMO

The purpose of this study was to assess the role of urinary IgG, serum CX3CL1 and miRNA 152-3p levels as predictors of nephropathy in type 2 Egyptian diabetic patients. Sixty type 2 diabetic patients and twenty healthy controls were enrolled in a cross-sectional study. Then they were grouped into: three groups based upon urine albumin excretion (UAE). The expression of miRNA 152-3p in serum was measured using quantitative polymerase chain reaction (RTq-PCR). Serum CX3CL1 and urinary IgG concentrations were measured by ELISA. RTq-PCR revealed that serum miRNA-152-3p levels in patients were significantly higher than in controls. There was significant differences between group with normoalbuminuria and groups with diabetic nephropathy DN as regard to age, duration of nephropathy, Albumin/Creatinine ratio (A/C ratio), creatinine, urine IgG, CX3CL1 and HbA1c. In diabetic patients, there was a significant positive correlation between miRNA-152-3p levels and disease duration only as well as significant positive correlations between urinary IgG levels and age, disease duration, serum creatinine, A/C ratio, and urea. Positive correlation between serum fractalkine CX3CL1 level and age, duration of disease, urea, creatinine, A/C ratio, HbA1C and IgG in patient with DN. Serum CX3CL1 level, urinary IgG were significantly increased with the progress of nephropathy so these integrated biomarkers could be used as good predictors for early identification of nephropathy. But miRNA- 152-3p has inadequate prognostic indicator for ESRD progression.


Assuntos
Quimiocina CX3CL1 , Diabetes Mellitus Tipo 2 , Nefropatias Diabéticas , MicroRNAs , Albuminas , Quimiocina CX3CL1/sangue , Creatinina/urina , Estudos Transversais , Diabetes Mellitus Tipo 2/complicações , Diabetes Mellitus Tipo 2/urina , Nefropatias Diabéticas/genética , Nefropatias Diabéticas/urina , Egito , Humanos , Imunoglobulina G/urina , MicroRNAs/sangue , Ureia
5.
Infect Drug Resist ; 14: 5375-5382, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34934331

RESUMO

INTRODUCTION: Neonatal sepsis can quickly progress to multi-organ failure with high morbidity and mortality, making early diagnosis mandatory. Although being the gold standard, the long duration of blood culture may lead to hazardous neonatal complications. Sepsis activates monocytes and changes their subset distribution with the resultant activation of lymphocytes and adaptive immune cells changing the plasma cytokines levels. SUBJECTS AND METHOD: Percentages of monocytes subsets, pattern of monocytes surface CD86 expression and serum IL-17 compared to serum procalcitonin were measured in 30 neonates with early sepsis and compared with age and sex matched 30 apparently health neonates as a control group. RESULTS: Gestational age, neonatal weight and hemoglobin concentration were significantly low in septic neonates vs the control group. Percentages of intermediate, nonclassical and CD86 positive monocytes, the mean fluorescence intensity of CD16 on CD16 positive monocytes, and serum levels of CRP, IL-17 and procalcitonin were significantly increased in septic neonates compared with the control group. CONCLUSION: Early neonatal sepsis was associated with increasing the percentage of CD86 positive monocytes. Serum IL-17 levels were positively correlated with increased serum procalcitonin.

6.
J Egypt Public Health Assoc ; 79(1-2): 13-29, 2004.
Artigo em Inglês | MEDLINE | ID: mdl-16916047

RESUMO

In the present study, stool samples were obtained from 575 individuals from El-Prince, a suburban village of Alexandria, Egypt to detect helminthic eggs and protozoan cysts. The results showed that, 35.7% of the sample had at least one vegetable-transmitted parasite, the highest percentage (46.4%) was in the age group of 6 to less than 15 years. The nematode Ascaris lumbricoides was the most prevalent in 18.4%, followed by the protozoan Giardia lamblia in 10.4%. Fasciola eggs were found in 2.4% of samples. Knowledge and practices of housewives concerning Fasciola and its source of transmission and methods of washing leafy vegetables was obtained through house-to-house interviews with 303 housewives. Knowledge that leafy vegetables were a source of Fasciola infection was indirectly proportional with better in younger housewives and those of secondary or higher education. More than half of the interviewed housewives (57.7%) washed leafy vegetables under running tap water and 32.7% soaked them in tap water. Only 9.6% soaked them in water mixed with a substance as vinegar, lemon juice or common salt. Only 5% of those who were infected with vegetable transmitted parasites washed vegetables by soaking in water with an added substance compared to 19.6% of parasite free housewives. Most of those adding a substance to soaking water (89.7%) used vinegar. Results of the study revealed that a serious and consistent effort through public health activities is essential to educate housewives about vegetable-transmitted parasites, their transmission and ways of prevention.


Assuntos
Conhecimentos, Atitudes e Prática em Saúde , Cônjuges , Verduras/parasitologia , Adulto , Animais , Egito , Fasciola/isolamento & purificação , Feminino , Humanos , Nematoides/isolamento & purificação
7.
J Egypt Public Health Assoc ; 77(5-6): 537-52, 2002.
Artigo em Inglês | MEDLINE | ID: mdl-17216977

RESUMO

The present work was implemented to determine the current status of Schistosoma mansoni infection in "El-Prince" village, near Alexandria, which was studied before as a control village between 1985 and 1990. Stool examination was performed on 571 of the inhabitants by Kato thick smear technique and intensity of infection was determined. Prevalence was found to be 15.4% with a percentage decrease of 53% from the 1990 survey. The geometric mean egg count (GMEC) ranged from 19.05 eggs/gram of stools in children aged five years or less to 81.86 in the oldest group greater than 50 years of age and averaged 42.26. Age stratified prevalence of infection peaked at 31.4% in the (20 to 25)-year-old age group. High prevalence of 23.6% was also observed in the age group from 36 to 50 years. Infection in males was higher than females (17.8% vs.13.5%). However the difference was not statistically significant (chi2 = 2.2, P = 0.0086). Risk factors significantly associated with the infection were an age more than 5 years, male gender, males with water contact activities and having done a previous laboratory analysis for schistosomiasis. The community category of the village changed from high prevalence in 1990 to moderate prevalence in 2002. Most of the infected individuals (85%) had light intensity of infection (less than 99 eggs /g stools). Active screening and treatment programs should be maintained in the area. More control measures should be directed towards older people who should be included in evaluation of control programs.


Assuntos
Esquistossomose mansoni/epidemiologia , Adolescente , Adulto , Animais , Criança , Pré-Escolar , Egito/epidemiologia , Fezes/parasitologia , Feminino , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Contagem de Ovos de Parasitas , Esquistossomose mansoni/parasitologia
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