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1.
Braz. j. biol ; 82: e235612, 2022. tab
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1153466

RESUMO

Abstract The experiment was carried out on mango cv. Dusehri to investigate the effect of N, P and K fertilizers on vegetative, reproductive growth, yield and fruit quality. Eight different fertilizer combinations such as T1 (control), T2 (N), T3 (P), T4 (K), T5 (NP), T6 (NK), T7 (PK) and T8 (NPK) were used. Individual or combine fertilizer application of N (1000 g), P (750 g) and K (750 g) were applied during growing season in February and August. All the treatments significantly influenced on vegetative growth, flowering, fruiting, yield and other physiochemical attributes of mango as compared to control. Least effect was observed with individual fertilizer application while combine fertilizer treatments enhanced most of the investigated parameters. Especially, qualitative traits showed non-significant differences between treated and untreated mango trees. However, among the different treatments T8 (NPK) showed significance for fruiting aspects such as maximum size of growth flushes (177.51 mm), total number of panicles/tree (845), total number of flowers/panicle (974), sex ratio (69.18%), fruit retention (13.85%), total number of fruits/tree (379), yield (82 kg/tree), fruit weight (197.5 g), pulp weight (135.5 g) and physiochemical parameters namely TSS (24.53), Vit. C (57.63 mg/100 mL) and total sugar (20.84%). In general, combine application of NPK (T8) were the most effective in enhancing fruiting aspects, yield, physiochemical characteristics as well as improved fruit quality of mango trees.


Resumo O experimento foi realizado em manga cv. Dusehri para investigar o efeito dos fertilizantes N, P e K no crescimento vegetativo, reprodutivo, produtividade e de qualidade do fruto. Foram utilizadas oito combinações diferentes de fertilizantes: T1 (controle), T2 (N), T3 (P), T4 (K), T5 (NP), T6 (NK), T7 (PK) e T8 (NPK). Cada tratamento de N (1.000 g), P (750 g) e K (750 g) foi aplicado duas vezes durante a estação de crescimento em fevereiro e agosto. Todos os tratamentos influenciaram significativamente o crescimento vegetativo, floração, frutificação, produtividade e outros atributos físico-químicos da manga em relação ao controle. Menos efeito foi observado com a aplicação individual de fertilizante, enquanto os tratamentos combinados aumentaram a maioria dos parâmetros investigados. Especialmente as características qualitativas mostraram diferenças não significativas entre mangueiras tratadas e não tratadas. No entanto, entre os diferentes tratamentos, T8 (NPK) apresentou significância para aspectos de frutificação, como tamanho máximo de folgas de crescimento (177,51 mm), número total de panículas/árvore (845), número total de flores/panícula (974), razão sexual (69,18%), retenção de frutos (13,85%), número total de frutos/árvore (379), produção (82 kg/árvore), peso do fruto (197,5 g) e peso da polpa (135,5 g), além de parâmetros físico-químicos, como TSS (24,53), vitamina C (57,63 mg/100 mL) e açúcar total (20,84%). Em geral, a aplicação combinada de NPK (T8) foi a mais eficaz no aprimoramento dos aspectos de frutificação, produtividade, características físico-químicas, além da melhoria da qualidade dos frutos das mangueiras.

2.
Braz J Biol ; 82: e235612, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33681899

RESUMO

The experiment was carried out on mango cv. Dusehri to investigate the effect of N, P and K fertilizers on vegetative, reproductive growth, yield and fruit quality. Eight different fertilizer combinations such as T1 (control), T2 (N), T3 (P), T4 (K), T5 (NP), T6 (NK), T7 (PK) and T8 (NPK) were used. Individual or combine fertilizer application of N (1000 g), P (750 g) and K (750 g) were applied during growing season in February and August. All the treatments significantly influenced on vegetative growth, flowering, fruiting, yield and other physiochemical attributes of mango as compared to control. Least effect was observed with individual fertilizer application while combine fertilizer treatments enhanced most of the investigated parameters. Especially, qualitative traits showed non-significant differences between treated and untreated mango trees. However, among the different treatments T8 (NPK) showed significance for fruiting aspects such as maximum size of growth flushes (177.51 mm), total number of panicles/tree (845), total number of flowers/panicle (974), sex ratio (69.18%), fruit retention (13.85%), total number of fruits/tree (379), yield (82 kg/tree), fruit weight (197.5 g), pulp weight (135.5 g) and physiochemical parameters namely TSS (24.53), Vit. C (57.63 mg/100 mL) and total sugar (20.84%). In general, combine application of NPK (T8) were the most effective in enhancing fruiting aspects, yield, physiochemical characteristics as well as improved fruit quality of mango trees.


Assuntos
Mangifera , Animais , Aves , Fertilizantes , Frutas , Árvores
4.
J Biol Regul Homeost Agents ; 33(6): 1891-1895, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31823594

RESUMO

INTRODUCTION: Hypothyroidism is a condition characterized by reduced circulating levels of thyroid hormones, T3 (tri-iodothyronine) and T4 (tetra-iodothyronine) which are produced by the thyroid gland, and increased levels of Thyroid Stimulating Hormone (TSH) from the anterior pituitary (1). The cardiovascular system is among the main targets of thyroid hormones (2), therefore, most descriptive signs and symptoms of thyroid disorders are due to their effects on the cardiovascular system (3). Direct actions of altered thyroid hormones on heart and indirect impacts through inflammatory markers, such as C-reactive protein (CRP), Interleukin-6 (IL-6) and Homocysteine (Hcy), are of great health concern. Particularly, CRP is a renowned biomarker to measure cardiovascular risks in both healthy as well as diseased subjects (4). Moreover, IL-6 is a chief mediator of tissue injury and infection, perpetuating lymphocyte infiltration in thyroid, manifesting its inflammation (5). Additionally, Hcy, having an atherogenic nature, stimulates inflammatory chemokine production and induces oxidative stress by lowering serum anti-oxidants (6). Hence, inflammatory markers may reveal the mechanism of disease progression, and can be used as a possible tool for predicting atherosclerosis and cardiovascular events (7). The aim of the present study was to assess the risk of cardiovascular dysfunctions in hypothyroid patients through predictive inflammatory markers.


Assuntos
Doenças Cardiovasculares/diagnóstico , Hipotireoidismo/complicações , Inflamação/sangue , Biomarcadores/sangue , Proteína C-Reativa/análise , Doenças Cardiovasculares/sangue , Homocisteína/sangue , Humanos , Interleucina-6/sangue
5.
Poult Sci ; 97(10): 3718-3727, 2018 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-29897547

RESUMO

The current study was carried out to investigate the consequences of mannan-oligosaccharides (MOS), a prebiotic, as feed supplement on biochemical parameters of Japanese quail breeders (Coturnix coturnix japonica). Twelve-week-old, 960 birds, belonging to 4 close-bred flocks (CBF), were randomly selected and divided into 4 treatment groups with 3 replicates according to pattern (4 strains × 4 treatment groups × 3 replicates). Birds were fed corn-based basal diet or the same diet supplemented with 0.25, 0.50, and 1.0% MOS for 15 wk. After termination of the experimental trial, samples of blood were taken from randomly selected 3 birds/replicate (1♂ and 2♀) and processed for serologic analysis. The statistical analysis was performed by applying 2-way analysis of variance. The serum concentrations of total proteins, globulin, high-density lipoprotein, and tri-iodothyronine (T3) of Japanese quail breeders were significantly (P < 0.05) higher in MOS-supplemented groups than that of control group; however, triglycerides were decreased significantly (P < 0.05) by MOS supplementation in female birds. Estrogen level in male birds was significantly (P < 0.05) different; however, non-significant differences were recorded in thyroxine (T4), progesterone, alanine aminotransferase, aspartate aminotransferase, glucose, albumin, cholesterol, and low-density lipoprotein concentrations in all feeding groups. The significant differences in biochemical parameters by feeding MOS-supplemented diets strengthened our hypothesis that the use of MOS may positively affect the health of Japanese quail breeders.


Assuntos
Coturnix/fisiologia , Mananas/metabolismo , Oligossacarídeos/metabolismo , Prebióticos/análise , Ração Animal/análise , Animais , Cruzamento , Coturnix/sangue , Dieta/veterinária , Feminino , Masculino , Mananas/administração & dosagem , Oligossacarídeos/administração & dosagem , Paquistão
6.
Opt Express ; 25(22): 27533-27542, 2017 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-29092224

RESUMO

We demonstrate a low noise bidirectional broadband distributed Raman pumping scheme combining dual order co-propagated pumps without increasing the signal RIN level. The noise performance improvement is compared experimentally and numerically with conventional counter-pumping only and bidirectional pumping with only a 2nd order co-pump for a 70nm bandwidth and 61.5km distributed Raman amplifier. The proposed broadband pumping scheme shows 1.2dB maximum noise figure improvement and extends the long-haul transmission reach up to 6150km with a Q-factor improvement of ~0.7dB compared with counter-pumping only scheme.

7.
Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi ; 31(10): 744-748, 2017 May 20.
Artigo em Chinês | MEDLINE | ID: mdl-29771034

RESUMO

Objective:The aim of this study is to introduce a method in classifying the REM related OSA (RrOSA) and the potential clinical significance. Method:Two hundred and fifty patients (male: 219) with OSAS diagnosed by polysomnographywere recruited in the study. The RrOSAS was defined as the ratio of AHIs in REM and non REM sleep (R) greater than one. In RrOSAS group, patients were divided into RrOSAS Ⅰa (R>2, NREM_AHI<15), RrOSAS Ⅰb (R>2, NREM_AHI>15), and RrOSAS II (2>R>1) three subtypes. Parameters of PSG, ESS, BMI and neck circufernces were obtained for further analysis. In RrOSA Ⅰa group, the efficiencies of CPAP and AutoPAP were investigated. Result:①There was a significant difference in gender between RrOSA and NRrOSA groups (P<0.05); ②There was no significant differences in ESS and BMI among the three subtypes of RrOSA (P>0.05); ③There was no significant difference in REM_AHI and REM_ODI between RrOSA and NRrOSA groups. Conclusion:The new method for the RrOSA classification helps better understanding the development of OSAS and might be useful in planning the CPAP treatment. RrOSA may be a special type of OSA, which may develop into other types as the condition develops.


Assuntos
Apneia Obstrutiva do Sono/fisiopatologia , Fases do Sono , Pressão Positiva Contínua nas Vias Aéreas , Feminino , Humanos , Masculino , Pescoço/anatomia & histologia , Polissonografia
8.
Genet Mol Res ; 15(4)2016 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-27808360

RESUMO

Cotton germplasm was analyzed to investigate its potential for developing water stress tolerance in varieties in the future. Four tolerant (NIAB-78, CIM-482, BH-121, and VH-142) and four susceptible (CIM-446, FH-1000, FH-900, and FH-901) lines were identified of 50 accessions based on their seedling root length. A complete set of diallel crosses among eight selected genotypes was subjected to genetic analysis for fiber property traits. Additive and non-additive genetic variance was involved in the inheritance of fiber strength, fineness, and length under normal and drought conditions. A large proportion of genetic variance was additive, which was further supported by moderately high narrow-sense heritability estimates for the characters. Graphic representation of variance versus covariance also depicted additive gene activity with partial dominance and the absence of non-allelic interactions in trait inheritance. The results of this study suggest that drought tolerance of cotton genotypes can be improved through crosses among tolerant genotypes using conventional selection procedures in segregating generations.


Assuntos
Adaptação Fisiológica/genética , Fibra de Algodão , Secas , Gossypium/genética , Característica Quantitativa Herdável , Alelos , Desidratação , Variação Genética , Modelos Genéticos
9.
Biochemistry (Mosc) ; 75(3): 367-74, 2010 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-20370615

RESUMO

We have studied the effect of 2,2,2-trifluoroethanol (TFE), an alpha-helix inducer, versus methyl cyanide (MeCN), a beta-sheet inducer, on acid-denatured human serum albumin (HSA) using far-UV circular dichroism, intrinsic fluorescence, 1-anilino-8-naphthalene sulfonate binding, and acrylamide quenching studies. Interestingly, at pH 2.0, where the recovery and resolution of the protein in reverse phase chromatography is high, its secondary structure remains unchanged even in the presence of very high concentration (76% v/v) of MeCN. Gain of 23 and 34% alpha-helicity was observed in the presence of 20 and 50% TFE, respectively. At pH 7.3, HSA aggregates in the presence of 40% MeCN, but it remains soluble up to 75% MeCN at pH 2.0. The results seem to be important for HSA isolation and purification.


Assuntos
Acetonitrilos/farmacologia , Albumina Sérica/química , Dicroísmo Circular , Humanos , Estrutura Secundária de Proteína , Espectrometria de Fluorescência , Espectrofotometria Ultravioleta , Trifluoretanol/química
10.
Genet Mol Res ; 7(4): 1408-13, 2008 Dec 23.
Artigo em Inglês | MEDLINE | ID: mdl-19224458

RESUMO

The genetic diversity among eight sunflower lines was determined through the estimation of the random amplified polymorphic DNA method. One hundred and fifty-six DNA fragments were generated by 20 random primers, for an average of about 7.8 bands per primer. Of these amplified DNA fragments, 104 were polymorphic among the eight sunflower lines. Nei and Li's similarity matrix gave values from 51.59 to 77.78%, which indicated a broad genetic base. The maximum similarity, 77.78%, was observed between R-SIN-82 and RN-46. The lowest similarity, 51.59%, was observed between the exotic lines CM-612 and HA-27. After knowing the knowledge of genetic diversity based on these random amplified polymorphic DNA markers, highly diverse lines can be used for further breeding programs to develop an ideal local hybrid of sunflower.


Assuntos
Variação Genética , Helianthus/genética , Técnica de Amplificação ao Acaso de DNA Polimórfico/métodos , DNA de Plantas/genética , Genótipo , Helianthus/classificação , Filogenia , Polimorfismo Genético
11.
Leukemia ; 20(4): 620-6, 2006 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-16437134

RESUMO

Biphenotypic acute leukemia (BAL) is a rare, difficult to diagnose entity. Its identification is important for risk stratification in acute leukemia (AL). The scoring proposal of the European Group for the Classification of Acute Leukemia (EGIL) is useful for this purpose, but its performance against objective benchmarks is unclear. Using the EGIL system, we identified 23 (3.4%) BAL from among 676 newly diagnosed AL patients. Mixed, small and large blast cells predominated, with FAB M2 and L1 constituting the majority. All patients were positive for myeloid (M) markers and either B cell (B) (17 or 74%) or T cell (T) (8 or 34%) markers with two exceptional patients demonstrating trilineage phenotype. Six (50%) of studied M-B cases were positive for both IGH and TCR. In six (26%) patients myeloid lineage commitment was also demonstrable by electron cytochemistry. Abnormal findings were present in 19 (83%) patients by cytogenetics/FISH/molecular analysis as follows: t(9;22) (17%); MLL gene rearrangement (26%); deletion(6q) (13%); 12p11.2 (9%); numerical abnormalities (13%), and three (13%) new, previously unreported translocations t(X;6)(p22.3;q21); t(2;6)(q37;p21.3); and t(8;14)(p21;q32). In conclusion, the EGIL criteria for BAL appear robust when compared against molecular techniques that, if applied routinely, could aid in detecting BAL and help in risk stratification.


Assuntos
Aberrações Cromossômicas , Cromossomos Humanos/genética , Análise Citogenética/métodos , Leucemia/diagnóstico , Leucemia/genética , Doença Aguda , Adolescente , Adulto , Idoso , Linhagem da Célula , Criança , Pré-Escolar , Estudos de Coortes , Análise Mutacional de DNA , Feminino , Rearranjo Gênico , Guias como Assunto , Humanos , Hibridização in Situ Fluorescente/métodos , Técnicas In Vitro , Lactente , Leucemia/classificação , Masculino , Proteína de Leucina Linfoide-Mieloide/genética , Fenótipo , Fatores de Risco , Sensibilidade e Especificidade
14.
Diagn Cytopathol ; 22(1): 3-6, 2000 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-10613963

RESUMO

Loss of chromosomes 1, 2, 6, 10, 13, 17, and 21 is a characteristic finding in chromophobe renal-cell carcinoma (ChRCC). Previously, cytogenetic and molecular genetic techniques were used in demonstrating the chromosomal monosomies in ChRCCs. We performed interphase fluorescent in situ hybridization (FISH) using centromeric probes for chromosomes 1, 2, 6, and 10 on touch imprint smears from six histologically proven ChRCCs. All six ChRCC tumors showed one FISH signal corresponding to one copy number for each of these chromosomes. The percent cells with one FISH signal ranged from 48-88% (chromosome 1), 36-89% (chromosome 2), 26-98% (chromosome 6), and 64-99% (chromosome 10). In addition, 3 of the 6 cases were further studied with centromeric probes for chromosomes 13, 17, and 21. All three revealed monosomy of these three chromosomes. We conclude that interphase FISH performed on touch imprint smears is a relatively simple, rapid, and reliable method for detecting chromosome abnormalities which are specific for ChRCCs.


Assuntos
Carcinoma de Células Renais/genética , Hibridização in Situ Fluorescente , Neoplasias Renais/genética , Monossomia , Carcinoma de Células Renais/patologia , Cromossomos Humanos Par 1 , Cromossomos Humanos Par 10 , Cromossomos Humanos Par 13 , Cromossomos Humanos Par 17 , Cromossomos Humanos Par 2 , Cromossomos Humanos Par 21 , Cromossomos Humanos Par 6 , Estudos de Avaliação como Assunto , Humanos , Neoplasias Renais/patologia , Valor Preditivo dos Testes
15.
Ann Saudi Med ; 20(3-4): 214-7, 2000.
Artigo em Inglês | MEDLINE | ID: mdl-17322660

RESUMO

BACKGROUND: Fragile X syndrome is the most common cause of inherited mental retardation. Patients with fragile X syndrome show variable mental disability, typical long and narrow facial appearance with large ears and prominent fontanelle and frequent macro-orchidism. It is generally associated with a fragile site at Xq 27.3, which can be observed in the metaphase chromosome following selective culture conditions. At the molecular level, the fragile X syndrome is associated with an amplification of CGG repeat sequence of the FMR1 gene. The prevalence estimates are reported as one per 1500 males and one per 2500 females. Estimated prevalence rates of fragile X syndrome in different ethnic groups range from 0.4-0.8 per 1000 in males and 0.2-0.6 per 1000 in females. In this study, we have determined the frequency of fragile X-positive cases in 305 preselected patients. MATERIALS AND METHODS: Three hundred and five Saudi patients with mental retardation/developmental delay/clinical suspicion of fragile X syndrome were screened for fragile X chromosome by cytogenetic methods. The majority of patients (95.59%) screened were under the age of 20 years. RESULTS: Two hundred and ninety-nine patients (98.03%) were in the category of mild to moderate mental retardation. Twenty-four males (7.86%) and two females (0.65%) were found to express fragile X site at q27.3. The frequency of fragile X-positive cells in males ranged between 7% and 58% (mean 26+/-13.11), while in the females it was between 14% and 21% (mean 12.5+/-35), respectively. CONCLUSION: The frequency of fragile X positive cases found in this study is similar to other reports of fragile X syndrome in preselected patients.

16.
Diagn Cytopathol ; 21(2): 81-91, 1999 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-10425044

RESUMO

Fine-needle aspiration biopsy findings in small round cell malignant tumors of children are briefly reviewed. All of these tumors usually have characteristic cytomorphology which, when recognized, may lead to a definitive diagnosis. However, when these tumors are undifferentiated, morphologic criteria may not be sufficient for arriving at a correct diagnosis. A variety of ancillary studies including electron microscopy, immunohistochemistry and DNA ploidy, cytogenetics, and fluorescent in situ hybridization may provide valuable additional information for precise characterization of these neoplasms. Some of the ancillary studies may also be used for assigning these cases to prognostically significant subgroups. This information may also help in defining the most suitable chemotherapeutic regimens for these tumors. Since most of these special studies require only a small amount of cellular material, fine-needle aspiration biopsy is ideally suited for obtaining samples for these procedures. It is hoped that as cytogenetic and molecular techniques become available in many diagnostic laboratories, a comprehensive evaluation of aspiration biopsies from round cell malignant tumors, encompassing a precise pathologic diagnosis, determination of prognosis, and prediction of response to therapy, will be possible in a majority of cases. Diagn. Cytopathol. 1999;21:81-91.


Assuntos
Biópsia por Agulha , Carcinoma de Células Pequenas/patologia , Diferenciação Celular/fisiologia , Criança , Humanos , Imuno-Histoquímica , Linfoma/patologia , Neuroblastoma/patologia , Sarcoma/patologia , Manejo de Espécimes/métodos
17.
Ann Saudi Med ; 19(6): 495-500, 1999.
Artigo em Inglês | MEDLINE | ID: mdl-17277466

RESUMO

BACKGROUND: Cytogenetic and molecular genetic techniques have been used in demonstrating the chromosomal abnormalities which characterize specific subtypes of renal cell carcinoma (RCC). The aim of this study was to determine the efficiency of fluorescent in situ hybridization (FISH) technique in characterizing various subtypes of RCC based on the presence of specific chromosome abnormalities found in each RCC subtype. MATERIALS AND METHODS: FISH was performed on touch imprint smears from eight renal cell carcinomas histologically confirmed by established criteria. RESULTS: In four tumors with histologic features of chromophobe renal cell carcinoma (ChRCC), interphase FISH was performed using centromeric probes for chromosomes 1, 2, 6, 10, 12, 17 and 21. All four ChRCC tumors showed one FISH signal corresponding to one copy number for each of these chromosomes. Two papillary RCCs included in this study showed trisomy 7 and 17, and loss of chromosome Y, using the corresponding chromosome centromeric probes. Similarly, we tested two clear cell RCCs for chromosome 3 short arm deletion with DNA probe 3p21.3. Both tumors showed loss of 3p21.3 signal. CONCLUSION: We conclude that interphase FISH performed on touch imprint smears is a relatively simple, rapid and reliable method for detecting chromosome abnormalities which are specific for various subtypes of RCC.

18.
East Mediterr Health J ; 5(6): 1218-24, 1999 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-11924115

RESUMO

Major chromosome abnormalities are present in 0.65% of all neonates. Fluorescent in situ hybridization (FISH) is useful in diagnosing microdeletion syndromes that would otherwise be difficult to diagnose using standard cytogenetics. In this study, we used FISH analysis in the laboratory diagnosis of 4 patients with Prader-Willi Syndrome [del(15)(q11.2q12)], 4 patients with DiGeorge syndrome [del(22)(q11.2q11.23)] and 4 patients with Williams syndrome [del(7)(q11.23q11.23)]. High-resolution chromosome analysis in all these patients was either normal or inconclusive but all the syndromes were confirmed using FISH. We recommend cytogenetic analysis should always be supplemented with FISH to diagnose all cases suspected of a microdeletion syndrome.


Assuntos
Síndrome de DiGeorge/diagnóstico , Hibridização in Situ Fluorescente/métodos , Síndrome de Prader-Willi/diagnóstico , Síndrome de Williams/diagnóstico , Adolescente , Pré-Escolar , Deleção Cromossômica , Análise Citogenética/métodos , Análise Citogenética/normas , Síndrome de DiGeorge/sangue , Síndrome de DiGeorge/complicações , Síndrome de DiGeorge/epidemiologia , Síndrome de DiGeorge/genética , Diagnóstico Diferencial , Elastina/genética , Humanos , Hibridização in Situ Fluorescente/normas , Lactente , Cariotipagem , Metáfase/genética , Fenótipo , Síndrome de Prader-Willi/sangue , Síndrome de Prader-Willi/complicações , Síndrome de Prader-Willi/epidemiologia , Síndrome de Prader-Willi/genética , Sensibilidade e Especificidade , Síndrome de Williams/sangue , Síndrome de Williams/complicações , Síndrome de Williams/epidemiologia , Síndrome de Williams/genética
19.
Ann Saudi Med ; 18(4): 311-7, 1998.
Artigo em Inglês | MEDLINE | ID: mdl-17344680
20.
Ann Saudi Med ; 18(6): 495-6, 1998.
Artigo em Inglês | MEDLINE | ID: mdl-17344711
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