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1.
PLoS One ; 17(5): e0269043, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35622830

RESUMO

BACKGROUND: In infants, respiratory syncytial virus (RSV) infection occasionally causes severe symptoms requiring respiratory support; however, supportive care is the primary treatment. This study compared the use of respiratory support among infants with RSV infection treated with or without pranlukast. METHODS: This retrospective cohort study included infants aged <10 months with RSV infection who were admitted to three secondary level hospitals in Japan between 2012 and 2019. The infants were divided into two groups depending on whether they were treated with pranlukast. The primary outcome was the receiving respiratory support (high-flow nasal cannula, nasal continuous positive airway pressure, or ventilator). The secondary outcomes were the length of hospital stay, and the Global Respiratory Severity Score (GRSS) on starting respiratory support or at the time of the worst signs during hospitalization. We performed a propensity score-matched analysis. RESULTS: A total of 492 infants, including 147 propensity score-matched pairs, were included in the analysis. The use of respiratory support was significantly lower in infants treated with pranlukast (3.4% [5/147]) than those treated without pranlukast (11.6% [17/147]; P = 0.01). In the propensity score-matched analysis, pranlukast use was associated with a significantly lower chance of needing respiratory support (odds ratio: 0.27, 95% confidence interval: 0.08-0.79; P = 0.01); however, the length of hospital stay (median: 4 days) and the GRSS (median: 2.804 and 2.869 for infants treated with and without pranlukast, respectively) did not differ significantly between propensity score-matched pairs. CONCLUSIONS: Pranlukast use was associated with a reduced likelihood of requiring respiratory support in infants aged <10 months with RSV infection.


Assuntos
Infecções por Vírus Respiratório Sincicial , Cromonas , Hospitalização , Humanos , Lactente , Tempo de Internação , Infecções por Vírus Respiratório Sincicial/terapia , Estudos Retrospectivos
2.
Eur J Protistol ; 84: 125881, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35436681

RESUMO

Five new Triplumaria species were described from Asian elephants (T. avis, T. cryptopteron, T. takakoae, T. soichii, T. cataphracta) as well as three species described by Timoshenko and Imai (1995), namely T. asiatica, T. nucleocaudata, and T. ovina. The new species have distinct skeletal plate structures: T. avis and T. cryptopteron have the ventral wing, grooves lined with lobes, and posterior fin; T. takakoae, the dorsal oar-shaped stick; T. soichii, the ventral spine and dorsal turn back fringed with lobes; T. cataphracta, two rows of bollard-shaped lobes and two folds composed of a smooth edge and lobes. These eight Triplumaria species have various buccal infraciliary bands. Triplumaria avis, T. cryptopteron, T. asiatica, and T. nucleocaudata have the perivestibular polybrachykinety connected only to the right end of adoral polybrachykinety. Triplumaria takakoae and T. soichii have the perivestibular polybrachykinety that connects to both ends of adoral polybrachykinety and has a loop along the vestibular left slit. Triplumaria cataphracta and T. ovina have the vestibular polybrachykinety connected to the right end of the twisted adoral polybrachykinety. Triplumaria species are highly differentiated ciliates in elephants; 23 of the 28 species described so far have been found in Asian elephants.


Assuntos
Cilióforos , Elefantes , Animais
3.
Surg Neurol Int ; 13: 85, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35399901

RESUMO

Background: Following clipping surgery for intracranial aneurysm, computed tomography angiography (CTA) is often used to confirm complete aneurysm obliteration. However, artifacts from the titanium clips usually degrade the images around them. The ultra-high-resolution computed tomography (UHR-CT) system recently became available in clinical practice. Here, we report a case in which CTA using the UHR-CT system successfully pointed out a small aneurysmal remnant after the clipping surgery, which was validated by digital subtraction angiography. Case Description: A patient underwent clipping surgery for an unruptured aneurysm using two titanium alloy clips. CTA using the UHR-CT system demonstrated a small remnant aneurysm. Digital subtraction angiography confirmed the minor remnant. The UHR-CTA images were comparable to three-dimensional reconstructed images from the rotational angiography. Conclusion: We propose that UHR-CTA is a reliable postoperative assessment method for intracranial clipping surgeries.

4.
Microscopy (Oxf) ; 2022 Apr 19.
Artigo em Inglês | MEDLINE | ID: mdl-35438158

RESUMO

Quality control of special steel is accomplished through visual inspection of its microstructure based on microscopic images. This study proposes an "automatic-quality-level-estimation system" based on machine learning. Visual inspection of this type is sensory-based, so training data may include variations in judgments and training errors due to individual differences between inspectors, which makes it easy for a drop in generalization performance to occur due to overfitting. To deal with this issue, we here propose the preprocessing of inspection images and a data augmentation technique. Preprocessing reduces variation in images by extracting features that are highly related to the level of quality from inspection images. Data augmentation, meanwhile, suppresses the problem of overfitting when training with a small number of images by taking into account information on variation in judgment values obtained from on-site experience. While the correct-answer rate for judging quality level by an inspector was about 90%, the proposed method achieved a correct-answer rate of 92.5%, which indicates that the method shows promise for practical application.

5.
Arch Rehabil Res Clin Transl ; 4(1): 100179, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-35282152

RESUMO

Objective: To assess the status of 10 patients with advanced osteonecrosis of the femoral head who underwent mesenchymal stromal cell transplants and a 12-week rehabilitation program 10 years earlier. Design: Retrospective study. Setting: University clinical research laboratory. Participants: Patients (N=10) who had undergone mesenchymal stromal cell transplantation and rehabilitation for a single hip osteonecrosis of the femoral head 10 years prior to the current study were recruited by telephone. The average age was 31.7 years and all participants were men; radiographic stages were 3A in 6 patients and 3B in 4 patients before treatment. Intervention: A 12-week rehabilitation program with follow-up once every 1 to 2 years was performed after mesenchymal stromal cell transplantation. Main Outcome Measures: Radiographic analysis, clinical score, timed Up and Go test, hip function (range of motion, muscle strength), and Short Form-36 scores were assessed before treatment and 1 and 10 years after treatment. Results: Upon imaging, 5 hips were found to be stable (stable group) and 5 had progressed (progressed group); 2 of the latter group required a total hip arthroplasty. The pretreatment radiographic stage of the progressed group was more advanced than that of the stable group. Body mass index was higher in the progressed group than in the stable group. Hip function and clinical score at 1 and 10 years after treatment improved in the hips of 8 patients without total hip arthroplasty. There were no severe adverse events during the rehabilitation. Conclusions: The 12-week rehabilitation program and annual follow-up after mesenchymal stromal cell transplantation for osteonecrosis of the femoral head was associated with pain reduction, maintaining hip muscle strength, widening range of motion, and improving quality of life. The level and timing of weight-bearing and social activity should be planned according to the individual's lifestyle and body composition.

6.
Ultrasound Med Biol ; 48(2): 304-312, 2022 02.
Artigo em Inglês | MEDLINE | ID: mdl-34740495

RESUMO

This study was aimed at identifying the optimal initiation time of ultrasound (US) therapy for peripheral nerve regeneration after axonotmesis. Thirty-six rats with sciatic nerve crush injury were divided into four groups that received US irradiation initiated 1, 7 or 14 d after injury, or sham stimulation for 4 wk. Motor function analysis was conducted weekly; however, there was no significant improvement attributed to US treatment. Four weeks after injury, compound muscle action potential amplitude values of the group in which US irradiation was initiated 1 d after the injury exhibited significant improvement compared with the sham stimulation group. In addition, myelin sheath thickness was significantly greater in the 1-d group than in other groups. These results indicate that US treatment initiated 1 d after peripheral nerve injury promotes maximum regeneration.


Assuntos
Traumatismos dos Nervos Periféricos , Nervo Isquiático , Animais , Bainha de Mielina , Compressão Nervosa , Regeneração Nervosa , Traumatismos dos Nervos Periféricos/terapia , Ratos , Recuperação de Função Fisiológica
7.
Pathog Glob Health ; 116(2): 99-106, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-34029172

RESUMO

Information on age-based Taenia solium taeniasis prevalence is crucial for control of cysticercosis. T. solium taeniasis prevalence was determined for a village in Liangshan Prefecture, Sichuan Province, China that was co-endemic for T. solium, Taenia saginata asiatica, and Taenia saginata. Individuals who were Taenia egg-positive by stool microscopy and/or expelled tapeworms or proglottids post-treatment were diagnosed as having taeniasis. Infecting species was identified via multiplex PCR on tapeworm specimens or coproPCR followed by sequencing. In addition, initial stool samples from 10 children with taeniasis suspected of having spontaneous expulsion of tapeworms within the period between diagnosis and treatment were subject to species confirmation via coproPCR and sequencing. Of the 389 study subjects, 194 (49.9%) were diagnosed with taeniasis. Children (< 16 years of age) had a higher T. solium taeniasis prevalence (8.8%) than older individuals (2.5%) (P = 0.0127). Molecular analysis of initial stool samples from 7 of 10 children suspected of spontaneously passing tapeworms indicated 6 infections due to T. solium and 1 infection due to T. saginata. This study found that young children had a higher T. solium taeniasis prevalence than older individuals, providing additional support for the belief that adult T. solium likely has a relatively short lifespan compared to other Taenia species with human definitive hosts.

8.
Acta Trop ; 227: 106297, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-34968452

RESUMO

Historically, cysticercosis cases caused by infection with the larval stage of the Taenia solium tapeworm have occurred sporadically in Bali, with taeniasis carriers found primarily in villages located in the Kubu subdistrict of Karangasem. As Bali is a well-known tourist destination, living standards are relatively high on the island, except for an area located beneath the northeast slope of the active volcano Mt. Agung (Alt. 3031 m), which includes Kubu. Over the last 30 years, pigs originating from this area have been found with T. solium cysticerci, whereas pigs from other parts of the island have not been shown to be infected. Out of 108 individuals screened via fecal sample examination in Kubu during 2019, 3 cases of T. solium taeniasis (2.8%) were identified. There was no significant difference in taeniasis prevalence from surveys conducted in 2011-2016 (1.0%, 11/1089) (p = 0.123). Out of 110 humans and 140 pigs tested serologically in Kubu during 2019, no cases of cysticercosis were identified. This is in contrast to a seroprevalence of 4.1% (42/1025) in humans during 2011-2016 and a seroprevalence of 13.1% (43/329) in pigs during 2011-2013. Over the last decade, improved drinking water and sanitary systems have been employed in addition to health education targeting primary school children, including emphasis on washing hands before eating and after defecation. This review provides previously unpublished survey data and a historical overview of T. solium infection in Bali and offers guidance on best practices to ensure that remaining pockets of transmission are addressed.


Assuntos
Cisticercose , Doenças dos Suínos , Taenia solium , Taenia , Teníase , Animais , Cisticercose/epidemiologia , Cisticercose/prevenção & controle , Cisticercose/veterinária , Indonésia/epidemiologia , Estudos Soroepidemiológicos , Suínos , Doenças dos Suínos/epidemiologia , Doenças dos Suínos/prevenção & controle , Teníase/diagnóstico , Teníase/epidemiologia , Teníase/prevenção & controle
10.
No Shinkei Geka ; 49(6): 1151-1159, 2021 Nov.
Artigo em Japonês | MEDLINE | ID: mdl-34879336

RESUMO

The appeal of spinal surgery is that it can dramatically improve patients' symptoms. To achieve this goal, establishment of preoperative diagnosis based on neurological examination is important. In this article, we will outline some tips for neurological examination in spinal surgery. The exam starts with listening to the patient's complaints. Pay attention to the patient's symptoms which change in relation to posture or movement. The muscle strength tests, sensory exams, and deep tendon reflex testing should follow. It would be fascinating if your examination skills were good enough to identify the responsible lesion causing the patient's symptoms without referring to the MRI. Neurosurgeons will be able to enjoy blissful moments with their patients, if the patients are relieved from pain or other symptoms after surgery.


Assuntos
Procedimentos Neurocirúrgicos , Dor , Humanos , Imageamento por Ressonância Magnética , Exame Neurológico
11.
Surg Neurol Int ; 12: 528, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34754578

RESUMO

BACKGROUND: The subcallosal artery (ScA) is a single dominant artery arising from the anterior communicating artery. Its injury causes amnesia and cognitive disturbance. The conventional computed tomographic angiography (C-CTA) is a common evaluation method of the intracranial artery. However, to image tinny perforating arteries such as the ScA is technically demanding for C-CTA. The purpose of this study is to investigate whether the ultra-high-resolution CTA (UHR-CTA) could image the ScA better than C-CTA. UHR-CTA became available in clinical practice in 2017. Its novel features are the improvement of the detector system and a small X-ray focus. METHODS: Between April 2019 and May 2020, 77 and 49 patients who underwent intracranial UHR-CTA and C-CTA, respectively, were enrolled in this study. Two board-certified neurosurgeons participated as observers to identify the ScA based on UHR-CTA and C-CTA images. RESULTS: UHR-CTA and C-CTA detected the ScA in 56-58% and 30-40% of the patients, respectively. In visualization of the ScA, UHR-CTA was better than C-CTA (P < 0.05, Fisher's exact test). Between the two observers, the Cohen's kappa coefficient was 0.77 for UHR-CTA and 0.78 for C-CTA. CONCLUSIONS: UHR-CTA is a simple and accessible method to evaluate intracranial vasculature. Visualization of the ScA with UHR-CTA was better than that with C-CTA. The high quality of UHR-CTA could provide useful information in the neurosurgery field.

12.
Sci Rep ; 11(1): 21343, 2021 11 01.
Artigo em Inglês | MEDLINE | ID: mdl-34725431

RESUMO

Recently, trans-radial intervention has gained popularity as a common procedure to reduce hemorrhagic complications. However, the cuff-type hemostatic device (TR Band) previously used at our institution required 6 h to achieve hemostasis. Since July 2016, we have been using the VasoSTAT, a new hemostatic device that could achieve hemostasis in 4 h. In a verification study, we found that prolonged activated clotting time (ACT) was related to transient hemorrhage occurrence after the hemostatic procedure. Therefore, we designed a hemostatic protocol based on ACT and evaluated its efficacy. In this retrospective and observational study, 78 and 111 patients used the VasoSTAT and TR Band, respectively, from July 2015 to May 2017. In the VasoSTAT group, the ACTs were significantly lower in the hemostasis success (246 ± 46 s) than in the failure group patients (327 ± 59 s) (P < 0.01). Therefore, we applied the hemostatic protocol to 271 patients from May 2017 to March 2020. The hemostasis success rate was 96% in the post-protocol applied group patients, which was significantly higher than the 82% success rate in the pre-protocol applied group patients (P < 0.01). VasoSTAT resulted in adequate hemostasis in 4 h. Further, ACT was predictive of adequate hemostasis.


Assuntos
Hemorragia/terapia , Hemostasia , Técnicas Hemostáticas/instrumentação , Idoso , Idoso de 80 Anos ou mais , Feminino , Hemorragia/sangue , Humanos , Masculino , Pessoa de Meia-Idade , Intervenção Coronária Percutânea , Estudos Retrospectivos
13.
Neuroradiology ; 2021 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-34791541

RESUMO

PURPOSE: Intraoperative motor-evoked potential (MEP) monitoring is widely used in the neck clipping of cerebral aneurysms. Little is known regarding the usefulness of intraoperative MEP monitoring in endovascular aneurysm surgery. The purpose of this study was to validate the feasibility of intraoperative MEP monitoring during the coil embolization of anterior choroidal artery (AChA) aneurysms. METHODS: Clinical and angiographic data of consecutive patients who underwent coil embolization for unruptured AChA aneurysms with or without intraoperative MEP monitoring between January 2014 and December 2018 at our institute were abstracted and analyzed retrospectively. RESULTS: Twenty-three unruptured AChA aneurysms were treated. Eleven patients received MEP monitoring, and three of them experienced intraoperative reduction or disappearance of the MEP wave. Even during MEP changes, AChA filling showed no change in any of the three cases. Although one case with MEP monitoring encountered the disappearance of AChA filling, there was no change in MEP. This might be due to retrograde filling of the AChA from the anastomosis with the lateral posterior choroidal artery. AChA blood flow detected by angiography did not always reflect MEP status. When comparing the presence or absence of MEP monitoring, the volume embolization ratio of coiled aneurysms was significantly better in the MEP group. CONCLUSION: Intraoperative MEP monitoring during endovascular coiling for AChA aneurysms may be feasible. AChA blood flow detected by angiography does not always reflect MEP status.

14.
Cells ; 10(10)2021 09 27.
Artigo em Inglês | MEDLINE | ID: mdl-34685536

RESUMO

Duchenne muscular dystrophy (DMD) is a genetic disorder that results from deficiency of the dystrophin protein. In recent years, DMD pathological models have been created using induced pluripotent stem (iPS) cells derived from DMD patients. In addition, gene therapy using CRISPR-Cas9 technology to repair the dystrophin gene has been proposed as a new treatment method for DMD. However, it is not known whether the contractile function of myotubes derived from gene-repaired iPS cells can be restored. We therefore investigated the maturation of myotubes in electrical pulse stimulation culture and examined the effect of gene repair by observing the contractile behaviour of myotubes. The contraction activity of myotubes derived from dystrophin-gene repaired iPS cells was improved by electrical pulse stimulation culture. The iPS cell method used in this study for evaluating muscle contractile activity is a useful technique for analysing the mechanism of hereditary muscular disease pathogenesis and for evaluating the efficacy of new drugs and gene therapy.


Assuntos
Células-Tronco Pluripotentes Induzidas/metabolismo , Fibras Musculares Esqueléticas/metabolismo , Distrofia Muscular de Duchenne/fisiopatologia , Apoptose , Diferenciação Celular , Células Cultivadas , Humanos
15.
Cartilage ; 13(2_suppl): 153S-167S, 2021 12.
Artigo em Inglês | MEDLINE | ID: mdl-34474599

RESUMO

OBJECTIVE: Curcumin monoglucuronide (TBP1901) is highly water soluble and can convert to free form curcumin, which has pharmacological effects, on intravenous administration. This study aimed to investigate the effectiveness of TBP1901 intra-articular injections in an osteoarthritis (OA) rat model. METHODS: Sixty-four male Wistar rats (12 weeks old) who underwent destabilized medial meniscus (DMM) surgery were randomly separated into the TBP1901 injection or saline solution (control) injection group. They were sacrificed at 1, 2, 6, or 10 weeks postoperatively (weeks 1, 2, 6, and 10; n = 8 for each group). TBP1901 (30 mg/mL) or saline solution of 50 µL was injected into the knee joints twice a week during weeks 1 and 2 to investigate the effects in the acute phase of posttraumatic (PT) OA or once a week during weeks 6 and 10 to investigate it in the chronic phase of PTOA. Histology, immunohistochemistry, and micro-computed tomography were performed to evaluate the changes in OA. RESULTS: TBP1901 injections significantly reduced synovial inflammation at weeks 1 and 2, and tumor necrosis factor-α expression in the articular cartilage at week 6. The TBP1901 injections also significantly suppressed articular cartilage damage, subchondral bone (SB) plate thickening, SB plate perforation, and osteophyte formation at week 10. CONCLUSIONS: TBP1901 intra-articular injections suppressed synovial inflammation in the acute phase of PTOA in DMM rats. In the chronic phase, TBP1901 suppresses articular cartilage damage and regulates SB plate changes.


Assuntos
Cartilagem Articular , Curcumina , Osteoartrite , Animais , Cartilagem Articular/patologia , Curcumina/farmacologia , Injeções Intra-Articulares , Masculino , Osteoartrite/metabolismo , Ratos , Ratos Wistar , Microtomografia por Raio-X
16.
J Biosci Bioeng ; 132(4): 399-407, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34364783

RESUMO

Constructing three-dimensional (3D) tissues is an important process to improve cellular functions in tissue engineering. When transplanting artificially constructed tissues, a poor vascular network restricts oxygen and nutrient supplies to the tissue cells, which leads to cell death and reduced rates of tissue engraftment. Therefore, it is necessary to develop a system that builds a vascular network within 3D tissues. Here, we developed a hypoxia-responsive gene expression system for production of an angiogenic factor, vascular endothelial growth factor (VEGF), to improve hypoxia and nutrition deficiencies inside artificial 3D tissues. We demonstrated that cells into which the hypoxia-responsive VEGF gene expression system had been introduced autonomously controlled VEGF expression in a hypoxic stress-dependent manner. Next, we confirmed that VEGF expression within a 3D cell sheet was induced in response to a hypoxic environment in vitro. The genetically modified cell sheet was subcutaneously transplanted into mice to evaluate the feasibility of the hypoxia-responsive VEGF gene expression system in vivo. The results suggest that the hypoxia-responsive VEGF gene expression system is promising to prepare artificial 3D tissues in regenerative medicine.


Assuntos
Hipóxia , Fator A de Crescimento do Endotélio Vascular , Animais , Hipóxia Celular , Expressão Gênica , Hipóxia/genética , Camundongos , Fator A de Crescimento do Endotélio Vascular/genética , Fatores de Crescimento do Endotélio Vascular
17.
J Vis Exp ; (173)2021 07 05.
Artigo em Inglês | MEDLINE | ID: mdl-34279516

RESUMO

The pathophysiology of primary osteoarthritis (OA) remains unclear. However, a specific subclassification of OA in relatively younger age groups is likely correlated with a history of articular cartilage damage and ligament avulsion. Surgical animal models of OA of the knee play an important role in understanding the onset and progression of post-traumatic OA and aid in the development of novel therapies for this disease. However, non-surgical models have been recently considered to avoid traumatic inflammation that could affect the evaluation of the intervention. In this study, an intra-articular cartilage lesion rat model induced by in vivo cyclic compressive loading was developed, which allowed researchers to (1) determine the optimal magnitude, speed, and duration of load that could cause focal cartilage damage; (2) assess post-traumatic spatiotemporal pathological changes in chondrocyte vitality; and (3) evaluate the histological expression of destructive or protective molecules that are involved in the adaptation and repair mechanisms against joint compressive loads. This report describes the experimental protocol for this novel cartilage lesion in a rat model.


Assuntos
Cartilagem Articular , Osteoartrite , Animais , Condrócitos , Modelos Animais de Doenças , Inflamação , Articulação do Joelho , Ratos
18.
Yale J Biol Med ; 94(2): 343-349, 2021 06.
Artigo em Inglês | MEDLINE | ID: mdl-34211353

RESUMO

In this perspectives paper, we discuss fertilization strategies for Taenia saginata and Taenia saginata asiatica as well as heterogeneity in Taenia solium, the causative agent of human cysticercosis. Two different genotypes of T. solium (Asian and Afro/American) were confirmed by mitochondrial DNA analysis approximately two decades ago. Since then, outcrossings of the two genotypes have been identified in Madagascar where the two genotypes are distributed sympatrically. Outcrossings were confirmed by the presence of discordance between mitochondrial and nuclear DNA. Since multiple tapeworm infections are common in endemic areas, outcrossing events likely occur quite frequently. Therefore, mitochondrial DNA from T. solium specimens collected from humans and pigs in endemic areas should be analyzed. If variations are found between specimens, nuclear DNA analysis should be performed to confirm the presence of discordance between mitochondrial and nuclear genes. Additional outcrossings likely add complexity to understanding the existing genetic diversity. Serological surveys are also recommended since serodiagnostic glycoprotein can also differentiate between the two genotypes. Viable eggs from different genotypes or from hybrids of two different genotypes should be used for experimental infection of pigs or dogs in order to observe any pathological heterogeneity in cysticercosis development. Although genetic diversity of T. solium is expected to result in clinical heterogeneity of cysticercosis in humans and pigs, there is currently no evidence showing that this occurs. There are also no comparative experimental studies on this topic. Therefore, studies evaluating the link between parasite heterogeneity and clinical outcome are warranted.


Assuntos
Cisticercose , Taenia saginata , Taenia solium , Animais , Cisticercose/genética , DNA Mitocondrial/genética , Cães , Variação Genética/genética , Suínos , Taenia saginata/genética , Taenia solium/genética
19.
PLoS One ; 16(7): e0253532, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34197495

RESUMO

BACKGROUND: Respiratory syncytial virus (RSV) is a common cause of acute respiratory infection in children. One of the most important strategies for treatment of an RSV infection is to decide whether the patient needs respiratory support. This study aimed to assess the validity and clinical benefit of the Global Respiratory Severity Score (GRSS) and the Wang bronchiolitis severity score (WBSS) for clinical decision-making regarding providing respiratory support (high-flow nasal cannula, nasal continuous positive airway pressure, or ventilator) in infants with an RSV infection. STUDY DESIGN AND METHODS: This retrospective cohort study enrolled 250 infants aged under 10 months who were admitted to Atsugi City Hospital with an RSV infection between January 2012 and December 2019. The utility of these scores was evaluated for assessing the need for respiratory support through decision curve analysis by calculating the optimal GRSS and WBSS cut-offs for predicting the need for respiratory support. RESULTS: Twenty-six infants (10.4%) received respiratory support. The optimal cut-offs for the GRSS and the WBSS were 4.52 and 7, respectively. Decision curve analysis suggested that the GRSS was a better predictive tool than the WBSS if the probability of needing respiratory support was 10-40%. CONCLUSIONS: The GRSS was clinically useful in determining the need for respiratory support in infants aged under 10 months with an RSV infection.


Assuntos
Oxigênio/uso terapêutico , Respiração Artificial/métodos , Infecções por Vírus Respiratório Sincicial/terapia , Infecções Respiratórias/terapia , Índice de Gravidade de Doença , Cânula , Feminino , Humanos , Lactente , Recém-Nascido , Masculino , Vírus Sincicial Respiratório Humano/patogenicidade , Estudos Retrospectivos
20.
Cytotechnology ; 73(3): 353-362, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-34149171

RESUMO

Hepatoma cells are a promising cell source for the construction of bioartificial liver (BAL) systems owing to their high proliferative capability. However, their low liver function compared with primary hepatocytes is a major problem. In a previous study, we established a genetically modified hepatoma cell line, Hepa/8F5, in which eight liver-enriched transcription factor (LETF) genes were transduced into mouse hepatoma Hepa1-6 cells using a drug-inducible transactivator system. These cells proliferate actively under normal culture conditions, meaning that large quantities can be prepared easily. When the overexpression of the LETFs is induced by the addition of an inducer drug, cell growth stops and cell morphology changes with concomitant high expression of liver functions. However, the liver functions largely depend on the presence of the inducer drug, which must be continuously added to maintain these enhanced functions. In the present study, we attempted to modify the method of induction of LETF overexpression in Hepa/8F5 cells to remove the requirement for continual drug addition. To this end, we constructed a system in which the artificial transactivator was transcribed and amplified under the control of a heat-shock protein promoter, and introduced the system into the genome of Hepa/8F5 cells. In our modified cell line, heat-triggered LETF expression was confirmed to induce high liver function. After drug-screening of transfected cells, we established a hepatoma cell line (Hepa/HS), which exhibited high, heat-inducible liver functions. The Hepa/HS cells may represent a new cell source for hepatic studies such as the construction of BAL systems. SUPPLEMENTARY INFORMATION: The online version of this article (10.1007/s10616-021-00457-4) contains supplementary material, which is available to authorized users.

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