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1.
FEMS Immunol Med Microbiol ; 39(3): 257-64, 2003 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-14642311

RESUMO

The immune cross-reactivity between Trypanosoma cruzi, the protozoan that causes Chagas' disease, and Phytomonas serpens, a trypanosomatid that infects tomatoes, was studied. Sera from patients with Chagas' disease presented a strong reactivity with P. serpens antigens by conventional serological assays such as indirect immunofluorescence (IIF) and direct agglutination test (DAT), confirmed after cross-absorption experiments. The results show that this protozoan is highly immunogenic and that rabbit and mouse hyperimmune serum raised against T. cruzi or P. serpens was able to recognize both T. cruzi and P. serpens antigens in immunofluorescence and agglutination assays. The antigenic cross-reactivity between T. cruzi and P. serpens was also demonstrated in vivo. BALB/c mice immunized by the intraperitoneal or oral route with P. serpens and later challenged with a lethal inoculum of T. cruzi blood forms showed a significant decrease in parasitemia and increase in survival compared to controls. A practical implication of these findings is that the ingestion by humans or animals of living plant trypanosomatids present in naturally infected edible fruits could potentially prime the immune response to T. cruzi antigens and interfere with the development of T. cruzi infection.


Assuntos
Antígenos de Protozoários/imunologia , Doença de Chagas/imunologia , Trypanosoma cruzi/imunologia , Trypanosomatina/imunologia , Testes de Aglutinação , Animais , Doença de Chagas/parasitologia , Doença de Chagas/prevenção & controle , Reações Cruzadas/imunologia , Feminino , Técnica Indireta de Fluorescência para Anticorpo , Humanos , Imunização , Lycopersicon esculentum/parasitologia , Camundongos , Camundongos Endogâmicos BALB C , Parasitemia , Coelhos , Análise de Sobrevida
2.
Rev. Inst. Med. Trop. Säo Paulo ; 38(3): 233-240, May-Jun. 1996.
Artigo em Inglês | LILACS | ID: lil-320639

RESUMO

The most frequent form of acquisition of Chagas' disease in endemic areas was the transmission through the feces of contaminated triatominae. However, special attention should be paid in urban areas to transmission by blood transfusion, justifying the compulsory screening of blood donors. Early investigations at blood banks in the town of Londrina, Brazil, demonstrated that the seroprevalence of anti-Trypanosoma cruzi antibodies among blood donors was approximately 7.0 in the fifties. Further studies demonstrated practically the same seroprevalence until the eighties. In an attempt to obtain data about the real dimension of the seropositivity for anti-Trypanosoma cruzi antibodies in the region, the authors carried out a large-scale study on 45,774 serum samples from blood donors of the Hemocentro of Hospital Univesitário Regional do Norte do Paraná (HURNP), Universidade Estadual de Londrina. The immunological tests were done at the Division of Clinical Immunology of HURNP from May 1990 to December 1994. The serum samples were studied by the indirect hemagglutination assay (IHA, using kits commercially obtained from EBRAM) and by indirect immunofluorescence (IFI, using kits from LIO SERUM) with anti-human IgG conjugate (LABORCLIN). The results demonstrated that 643 serum samples were positive in both assay corresponding to a seroprevalence of 1.4, i.e., a significant decrease in anti-Trypanosoma cruzi antibodies in the region in comparison with the previously mentioned rates. Data correlating sex and age of seropositive blood donors are presented, as well as the possible factors that may have contributed to the results observed.


Assuntos
Humanos , Animais , Masculino , Feminino , Criança , Adolescente , Adulto , Pessoa de Meia-Idade , Anticorpos Antiprotozoários/sangue , Doadores de Sangue , Doença de Chagas/epidemiologia , Trypanosoma cruzi , Transfusão de Sangue , Brasil , Doença de Chagas/sangue , Doença de Chagas/transmissão , Hospitais Universitários , Estudos Soroepidemiológicos
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