Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 550
Filtrar
1.
Opt Express ; 28(21): 30964-31019, 2020 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-33115085

RESUMO

The mid-infrared (MIR) represents a large portion of the electromagnetic spectrum that is progressively being exploited for an enormous number of applications. Thermal imaging cameras, dental and skin resurfacing lasers, and narcotics detectors at airports are all mainstream examples involving the MIR, but potential applications of MIR technologies are much larger. Accessing the unique opportunities afforded by the MIR is critically dependent on the specific characteristics of MIR emitting sources that become available. In this review, we survey an important enabling technology to the opening up of MIR science and applications, namely that driven by fiber-based sources of coherent MIR radiation. In this review paper, we describe many of the key advances in the innovation and development of such sources over the past few decades and discuss many of the underlying science and technology issues that have resulted in specific recent source achievements, especially in light of new applications enabled by these new source capabilities. We also discuss a few specific anticipated future needs and some potentially disruptive approaches to future MIR fiber source development.

2.
Opt Express ; 28(15): 21522-21548, 2020 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-32752429

RESUMO

Glass ceramics (GCs), which consist essentially of a homogeneous solid state dispersion of nanocrystals (NCs) embedded in a chemically inert and mechanically robust glass matrix, appear to be an extremely promising class of solid state materials that can be easily tailored into arbitrary shapes, including a new generation of optical fibers, for efficient incoherent and coherent sources of mid-infrared (MIR) light emission. This unique capability not only stems from the fact that one can tailor the underlying glass matrix for optimal macroscopic physical properties and ultrahigh transparency at the wavelengths of interest (resulting in appropriate "transparent glass ceramics" or TGCs), but also stems from the fact that one can embed these matrices with size and structure-tailored NCs, which in turn can be doped with relatively high concentrations of MIR emitting rare-earth or transition metal ions. This potential is tantamount to the localization of these highly efficient MIR ionic emitters into carefully selected and highly favorable "process-engineered" custom crystalline host "nanocages," while insulating the ionic emitters from the emission-quenching glass host matrix, the latter being chosen largely because of its highly favorable macroscopic bulk properties, including its ductility and formability into near-arbitrary shapes (at appropriate temperatures). Such MIR TGCs appear to be very promising for numerous photonics applications, including compact and relatively efficient waveguide sensors, broadband incoherent MIR light sources, superluminescent light sources, advanced fiber-optic devices, and broadly wavelength-tunable and ultrashort pulse mode-locked fiber and bulk solid-state lasers. In this paper, we review past achievements in this field, starting with an overview of TGCs, followed by discussions of currently preferred methods of fabrication, characterization, and optimization of suitably doped oxyfluoride, tellurite, and chalcogenide TGCs and of our projections of anticipated future developments in this field at both the materials and device levels.

3.
J Med Eng Technol ; 44(6): 338-345, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32757800

RESUMO

Motion artefacts in electrocardiographic (ECG) signal are suppressed mainly by adaptive noise cancellation and wavelet denoising. While the former requires a motion sensor in addition to ECG electrodes, the latter removes some of the desired low-frequency components in the signal. In this paper spectral trimming technique is being introduced for suppressing the motion artefacts in stress electrocardiography. In this method, Fourier spectral coefficients up to 1.221 Hz of noisy signal are trimmed on the basis of template derived from resting ECG signal in the same subject. The proposed spectral trimming technique has yielded the lowest value of mean ± standard deviation for root mean square error (18.92 ± 8.71) and highest value of the signal to noise ratio (6.439 ± 4.266) as compared to other three methods, namely adaptive noise cancellation, wavelet decomposition and adaptive line enhancement with compatible value of correlation coefficient. Subsequently, spectral trimming technique has been implemented in real-time (deferred by 8.2 s) application for stress electrocardiography. Spectral trimming technique thus offers a method of choice for motion artefact suppression in offline as well as deferred online applications. This method takes care of the limitations of conventional methods such as adaptive noise cancellation or wavelet denoising for suppressing motion artefacts in stress electrocardiography.


Assuntos
Artefatos , Eletrocardiografia , Teste de Esforço , Processamento de Sinais Assistido por Computador , Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem
4.
Osteoporos Int ; 31(11): 2189-2196, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32623489

RESUMO

Opportunistic osteoporosis screening involves measuring the attenuation of L1 vertebrae on abdominal computed tomography (CT), which correlates with DXA T-score. We found that this approach is useful for detecting low bone mass in patients with diabetes and propose L1 attenuation ≤ 135 Hounsfield units (HU) as a threshold for which DXA should be strongly considered. INTRODUCTION: Attenuation of the L1 vertebrae on computer tomography (CT) images done for other reasons ("Opportunistic Osteoporosis Screening") has been found to correlate well with DXA-derived T-score. However, the method and the thresholds have never been tested specifically in those with diabetes mellitus (DM), in whom the fracture risk is greater than explained by BMD. METHODS: In a retrospective study of subjects with DM who had both abdominal CT and DXA within 6 months of each other, we compared L1 attenuation and DXA T-score to define the sensitivity and specificity of thresholds previously established in the general population. RESULTS: There were 313 subjects among whom 18 (5.8%) had prior major osteoporotic fracture (MOF). Subjects with MOF had lower T-scores (- 2.3 ± 1.4 vs. - 0.9 ± 1.4, p < 0.001) and L1 attenuation (104 HU ± 46 vs. 149 HU ± 47, p < 0.001) than non-fracture subjects. L1 attenuation ≤ 160 HU was 91% sensitive for osteoporosis, while ≤ 110 HU was 80% specific. For a higher T-score of ≤ - 1.5, L1 attenuation ≤ 135 HU showed balanced sensitivity and specificity (65% and 69%, respectively). CONCLUSION: Opportunistic osteoporosis screening with abdominal CT is useful in determining the need for DXA screening in subjects with diabetes. We propose L1 attenuation ≤ 135 HU as a reasonable threshold for detecting the T-score of ≤ - 1.5, which is likely associated with increased fragility in DM.


Assuntos
Doenças Ósseas Metabólicas , Diabetes Mellitus , Osteoporose , Tomografia Computadorizada por Raios X , Abdome/diagnóstico por imagem , Absorciometria de Fóton , Densidade Óssea , Doenças Ósseas Metabólicas/diagnóstico por imagem , Doenças Ósseas Metabólicas/epidemiologia , Doenças Ósseas Metabólicas/etiologia , Complicações do Diabetes , Humanos , Osteoporose/complicações , Osteoporose/diagnóstico por imagem , Estudos Retrospectivos
5.
Virus Res ; 280: 197903, 2020 04 15.
Artigo em Inglês | MEDLINE | ID: mdl-32105764

RESUMO

Soybean yellow mottle mosaic virus (SYMMV) is a newly identified member of the genus Gammacarmovirus from grain legumes in India. As the modes of transmission of this virus have not been described, we assessed the possibility of SYMMV to be transmitted through seed collected from field infected mungbean plants and mechanically sap inoculated French bean plants using serological and molecular techniques followed by progeny assays. Direct antigen coated enzyme linked immunosorbent assay (DAC-ELISA) and reverse transcription polymerase chain reaction (RT-PCR) results are inconsistent with field infected mungbean seed tissues to ensure seed transmissibility irrespective of seed number used. Seed from mechanical sap inoculated French bean showed higher absorbance values in DAC-ELISA and amplification corresponding to replicase, movement and coat protein regions of SYMMV genome. The relative accumulation of SYMMV was higher in pod walls, immature seed and stamens and stigma of mechanical sap inoculated French bean. Progeny assays with infected seed revealed the seed transmissibility of SYMMV at the rate of 63.33% in mungbeanand 73.33% in French bean. Mechanical sap inoculation of mungbean progeny seedlings on French bean cv. Pusa Parvati produced characteristic symptoms of SYMMV. The results obtained from this study demonstrate that SYMMV is seed borne in nature and can be transmitted to next generation seedlings. This is the first report of seed transmission of SYMMV in mungbean and French bean.


Assuntos
Doenças das Plantas/virologia , Sementes/virologia , Tombusviridae/genética , Vigna/virologia , Genoma Viral , Índia , Phaseolus/virologia , Filogenia , Plântula/virologia
6.
Indian J Otolaryngol Head Neck Surg ; 71(Suppl 3): 1734-1738, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31763235

RESUMO

Chronic rhinosinusitis a very common disease characterized by inflammation of the mucosa of the paranasal sinuses. Nasal endoscopy and computed tomography (CT) scans are successfully used as diagnostic modalities of nose and paranasal sinus diseases. CT scan has been traditionally considered as the gold standard for diagnosing Chronic rhinosinusitis. But high cost, radiation exposure and high rates of false positivity are important drawbacks. However, nasal endoscopy as an OPD tool helps in early categorization of patients for further evaluation by imaging. This is a cost cutting as well as time saving option. To compare nasal endoscopy and CT scan with respect to prediction of intrasinus involvement.To evaluate the role of nasal endoscopy as an effective alternative to CT-scan in diagnosing chronic rhinosinusitis.82 clinically diagnosed cases of chronic rhinosinusitis underwent CT scan and were advised to undergo diagnostic endoscopy. Both the modalities were compared for the diagnosis of chronic rhinosinusitis with focus on anatomic variations and the pathological findings in the nose and paranasal sinuses, and their correlation, specificity, sensitivity, positive predictive value and negative predictive values were calculated. The association between diagnostic endoscopy and CT was calculated using χ 2 test. Pearson χ 2 value: 8.173, P value = 0.004 (<0.05) i.e. significant. The sensitivity and specificity of nasal endoscopy was calculated with respect to diagnosing chronic rhinosinusitis in comparison to CT-scan. The results were: sensitivity was 78.08% (95% CI 66.57-86.58%), specificity was 66.67% (95% CI 30.91-90.95%) while the positive predictive value: 95% and the negative predictive value was 27.27%. CT scan is considered to be the gold standard for sinonasal imaging. Endoscopy can predict the intrasinus involvement in rhinosinusitis thereby reducing overdone CT-scans.

7.
Bioprocess Biosyst Eng ; 42(3): 367-377, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30470907

RESUMO

Production of laccase from Ganoderma lucidum RCK 2011 under solid-state fermentation (SSF) conditions was optimized using response surface methodology, resulting in an approximate eightfold increase compared to that in the unoptimized media. Further, the enzyme produced under SSF as whole fermented substrate (in situ SSF laccase) was found to be more stable than the in vitro enzyme (harvested by downstreaming processing of fermented wheat bran). Interestingly, the biobleaching potentials of both in situ and in vitro SSF laccases were comparable, saving 25% chlorine dioxide for achieving similar pulp brightness as obtained in the pulp treated chemically. The reduction in the demand of chlorine dioxide in the pulp bleaching sequence subsequently decreased the levels of adsorbable organic halogen (AOX) in the resulting effluents of the process by 20% compared to the effluents obtained from chemical bleaching sequence. Therefore, direct application of in situ SSF laccase in pulp biobleaching will be environmentally friendly as well as economical and viable for implementation in paper mills.


Assuntos
Proteínas Fúngicas , Lacase , Papel , Reishi/enzimologia , Proteínas Fúngicas/biossíntese , Proteínas Fúngicas/química , Lacase/biossíntese , Lacase/química
8.
Osteoporos Int ; 29(9): 2093-2099, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-29858633

RESUMO

The study investigates the association of trabecular bone score (TBS) and fractures among minority populations. In NHANES 2005-2008, TBS was associated with history of fractures in Caucasian subjects but demonstrated somewhat weaker associations in African American and Mexican American women. INTRODUCTION: Trabecular bone score (TBS), a textural analysis of the lumbar spine DXA image, predicts fractures well in Caucasian (CA) and Asian populations but is less well studied in African American (AA) and Mexican American (MA) subjects. It is not clear whether TBS is associated with or is predictive of fragility in these racial/ethnic groups. METHODS: We analyzed data from subjects from NHANES 2005-2008 over the age of 40 who had TBS: 1178 CA, 467 AA, and 397 MA women and 1200 CA, 502 AA, and 386 MA men. TBS was categorized into normal, ≥ 1.310, partially degraded < 1.310, and > 1.230, or degraded, ≤ 1.230. History of fracture was assessed by questionnaire. RESULTS: Among women, there was an increasing prevalence of fracture with worsening TBS category. However, when controlling for age, BMI, and low T-score, the association between TBS category and previous fracture was only significant in CA women (OR 1.49 per worsening category, 95% CI 1.20-1.85). In men, there was also an increase in the prevalence of fracture with worsening TBS category in all races/ethnicities. When controlling for age, BMI, and low T-score, the association between TBS category and previous fracture was only significant in CA men (OR 1.47 per worsening category, 95% CI 1.10-1.95), though analysis was somewhat limited by small fracture numbers. CONCLUSIONS: The association of fracture and TBS varies by race/ethnicity and gender with weaker association observed in AA and MA women. More research is needed to define the proper use of TBS for predicting fractures in minority groups.


Assuntos
Osso Esponjoso/fisiopatologia , Fraturas por Osteoporose/etnologia , Adulto , Afro-Americanos/estatística & dados numéricos , Americanos Asiáticos/estatística & dados numéricos , Feminino , Colo do Fêmur/fisiopatologia , Articulação do Quadril/fisiopatologia , Humanos , Vértebras Lombares/fisiopatologia , Masculino , Americanos Mexicanos/estatística & dados numéricos , Pessoa de Meia-Idade , Inquéritos Nutricionais , Osteoporose/etnologia , Osteoporose/fisiopatologia , Fraturas por Osteoporose/fisiopatologia , Estados Unidos/epidemiologia , /estatística & dados numéricos
9.
Virology ; 508: 108-117, 2017 08.
Artigo em Inglês | MEDLINE | ID: mdl-28527340

RESUMO

Groundnut bud necrosis virus induces necrotic symptoms in different hosts. Previous studies showed reactive oxygen species-mediated programmed cell death (PCD) resulted in necrotic symptoms. Transgenic expression of viral protein NSs mimics viral symptoms. Here, we showed a role for NSs in influencing oxidative burst in the cell, by analyzing H2O2 accumulation, activities of antioxidant enzymes and expression levels of vacuolar processing enzymes, H2O2-responsive microRNA 319a.2 plus its possible target metacaspase-8. The role of NSs in PCD, was shown using two NSs mutants: one in the Trp/GH3 motif (a homologue of pro-apototic domain) (NSsS189R) and the other in a non-Trp/GH3 motif (NSsL172R). Tobacco rattle virus (TRV) expressing NSsS189R enhanced the PCD response, but not TRV-NSsL172R, while RNA silencing suppression activity was lost in TRV-NSsL172R, but not in TRV-NSsS189R. Therefore, we propose dual roles of NSs in RNA silencing suppression and induction of cell death, controlled by different motifs.


Assuntos
Apoptose , Inativação Gênica , Doenças das Plantas/virologia , Tabaco/citologia , Tabaco/genética , Tospovirus/metabolismo , Proteínas não Estruturais Virais/química , Proteínas não Estruturais Virais/metabolismo , Motivos de Aminoácidos , Sequência de Aminoácidos , Peróxido de Hidrogênio/metabolismo , Dados de Sequência Molecular , Filogenia , Doenças das Plantas/genética , Explosão Respiratória , Alinhamento de Sequência , Tabaco/metabolismo , Tabaco/virologia , Tospovirus/química , Tospovirus/genética , Proteínas não Estruturais Virais/genética
10.
Malays Orthop J ; 11(1): 1-5, 2017 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-28435566

RESUMO

INTRODUCTION: The present study was performed to compare cemented total hip replacement (THR) with cemented bipolar prosthesis in the treatment of displaced fracture neck of femur in elderly patients. MATERIALS AND METHODS: This prospective study included 47 patients of greater than 60 years of age and having fracture of neck of femur, out of which 25 patients were managed by cemented bipolar prosthesis and remaining 22 were managed by cemented THR between June 2011 and June 2013. These patients were followed up post-operatively for two years, at 6, 12 and 24 months, for functional analysis using Modified Harris Hip Score. RESULTS: Modified Harris Hip Score was significantly higher in the THR group as compared to the bipolar prosthesis group at 6, 12 and 24 months post-operatively. Pain was almost similar in both the groups at all follow-up periods. Gait and range of motion was significantly higher in THR group as compared to bipolar prosthesis group at all-time point intervals. CONCLUSION: Cemented THR is a better option as compared to cemented bipolar prosthesis based on our short term functional outcome for the management of fracture of neck of femur in elderly patients.

11.
Asian Spine J ; 11(1): 31-36, 2017 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-28243366

RESUMO

STUDY DESIGN: Retrospective analysis. PURPOSE: We evaluated the functional, neurological, and radiological outcome in patients with thoracic and thoracolumbar tuberculosis operated through the transpedicular approach. OVERVIEW OF LITERATURE: For surgical treatment of thoracic and thoracolumbar tuberculosis, the anterior approach has been the most popular because it allows direct access to the infected tissue, thereby providing good decompression. However, anterior fixation is not strong, and graft failure and loss of correction are frequent complications. The transpedicular approach allows circumferential decompression of neural elements along with three-column fixation attained via pedicle screws by the same approach. METHODS: A total of 47 patients were diagnosed with tuberculosis of the thoracic or thoracolumbar region from August 2012 to August 2013. Of these, 28 patients had progressive neurological deterioration or increasing back pain despite conservative measures and underwent transpedicular decompression and pedicle screw fixation with posterior fusion. Antituberculosis therapy was given till signs of radiological healing were evident (9-16 months). Functional outcome (visual analog scale [VAS] score for back pain), neurological recovery (Frankel grading), and radiological improvement were evaluated preoperatively, immediate postoperatively, and at 3 months, 6 months, and 1 year. RESULTS: Mean VAS score for back pain improved from 8.7 preoperatively to 1.1 at 1 year follow-up. Frankel grading preoperatively was grade B in 7, grade C in 11, and Grade D in 10 patients, which improved to grade D in 6 and grade E in 22 patients at the last follow-up. Radiological healing was evident in the form of reappearance of trabeculae formation, resolution of pus, fatty marrow replacement, and bony fusion in all patients. Mean correction of segmental kyphosis postoperatively was 10.5°. Mean loss of correction at final follow-up was 4.1°. CONCLUSIONS: Transpedicular decompression with instrumented fusion is a safe and effective approach for management of patients with thoracic and thoracolumbar tuberculosis.

12.
J Assoc Physicians India ; 65(9): 43-47, 2017 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-29313576

RESUMO

Objective: This real-world, observational, prescription event monitoring study was conducted to evaluate safety and efficacy of indigenous tenecteplase (TNK-tPA) in Indian patients presenting with ST elevation myocardial infarction (STEMI). Methods: This is a multi-centric, observational, prescription event monitoring study. Data was collected for 7,668 patients from 1,307 investigator sites across India from January 2011 to February 2016. Results: Overall, 76.71% patients were hypertensive, 47.97% patients were diabetic, 42.01% had dyslipidemia, 24.35% had ischemic heart disease and 40.82% patients were smokers. The overall rate for achieving clinically successful thrombolysis by TNK was 93.34%. Delayed administration of tenecteplase yielded lower success rate (84.66%) as against those patients who received tenecteplase within 3 hours of symptoms (94.34%). 93.2% patients had chest pain resolution after pharmacological fibrinolysis. Overall 91.1% patients had 50% resolution of ST elevation at 90 minutes and mean time for 50% ST resolution was 72.06 minutes. Overall 53 patients died (mortality of 0.69%) before discharge. The incidence of bleeding (excluding stroke) was 1.77%, any stroke without ICH was 0.18% and any ICH was 0.38%. Conclusion: The findings of this study further reinforce the safety and efficacy of indigenous TNK-tPA in Indian patients presenting with STEMI, including high-risk sub-groups. The study also highlights the importance of early reperfusion therapy.


Assuntos
Fibrinolíticos/uso terapêutico , Reperfusão Miocárdica , Infarto do Miocárdio com Supradesnível do Segmento ST/tratamento farmacológico , Terapia Trombolítica , Ativador de Plasminogênio Tecidual/uso terapêutico , Feminino , Humanos , Índia/epidemiologia , Masculino , Pessoa de Meia-Idade , Infarto do Miocárdio com Supradesnível do Segmento ST/epidemiologia , Tenecteplase , Tempo para o Tratamento
13.
Osteoporos Int ; 28(3): 917-923, 2017 03.
Artigo em Inglês | MEDLINE | ID: mdl-27743070

RESUMO

Trabecular bone score, an indirect measure of bone structure, may differ between ethnicities. We found that African Americans had lower trabecular bone score than do whites referred for densitometry, even when controlling for age and abdominal soft tissue thickness. PURPOSE: Trabecular bone score (TBS), an indirect measure of bone structure, has been shown to predict fractures in predominantly white populations. Analysis of NHANES data revealed lower TBS in African Americans than in whites. However, it is not clear if this is true in patients referred for densitometry (where fracture risk stratification is most pertinent) or if ethnic differences in TBS may be related to differences in abdominal soft tissue (tissue thickness), which was not controlled for in the NHANES study. METHODS: We retrospectively analyzed all BMD scans obtained at a university hospital in Chicago between 2011 and 2016. RESULTS: There were 3187 women (51 % African American) and 675 men (32 % African American). African American women were older (69.6 ± 10.4 vs. 64.8 ± 1.3) and heavier (BMI 28.3 ± 4.7 vs. 25.4 ± 4.5) than whites were, while men were of similar age and BMI. African American women had higher T-scores at all sites (the lowest of T-scores, termed LowT, -1.5 ± 1.2 vs. -1.9 ± 1.0, p < 0.001) but lower TBS than white women even when adjusting for age and tissue thickness (1.231 ± 0.130 versus 1.251 ± 0.130, p < 0.001). While LowT was higher in African American men (-1.1 ± 1.2 vs. -1.5 ± 1.4, p < 0.001), TBS was lower than in white men even after adjusting for age and tissue thickness (1.232 ± 0.144 vs. 1.275 ± 0.144, p < 0.001). CONCLUSIONS: African Americans had lower TBS than whites did, even with adjustment for age and tissue thickness. Ethnic differences in TBS should be considered when assessing fracture risk in clinical practice.


Assuntos
Afro-Americanos/estatística & dados numéricos , Densidade Óssea/fisiologia , Osso Esponjoso/fisiopatologia , Osteoporose/etnologia , /estatística & dados numéricos , Absorciometria de Fóton/métodos , Idoso , Idoso de 80 Anos ou mais , Índice de Massa Corporal , Chicago/epidemiologia , Feminino , Colo do Fêmur/fisiopatologia , Articulação do Quadril/fisiopatologia , Humanos , Vértebras Lombares/fisiopatologia , Masculino , Pessoa de Meia-Idade , Osteoporose/diagnóstico , Osteoporose/fisiopatologia , Estudos Retrospectivos
14.
Artigo em Inglês | MEDLINE | ID: mdl-26728805

RESUMO

BACKGROUND: Melasma, which is fairly common in Indians, causes significant emotional and psychological impact. A Hindi instrument would be useful to assess the impact of melasma on the quality of life in Indian patients. OBJECTIVE: To create a semantic equivalent of the original MELASQOL questionnaire in Hindi and validate it. METHODS: A Hindi adaptation of the original MELASQOL (Hi-MELASQOL) was prepared using previously established guidelines. After pre-testing, the Hi-MELASQOL questionnaire was administered to 100 women with melasma visiting the out-patient registration counter of Safdarjung Hospital, Delhi. These women were also administered a Hindi equivalent of the Health Related Quality of Life (HRQOL) questionnaire. Melasma area severity index (MASI) of all the participants was calculated. RESULTS: The mean MASI score was 20.0 ± 7.5 and Hi-MELASQOL score was 37.19 ± 18.15; both were highly, positively and significantly correlated. Reliability analysis showed satisfactory results. Physical health, emotional well-being and social life were the most adversely affected life domains. LIMITATIONS: It was a single-center study and the number of patients studied could have been larger. CONCLUSION: Hi-MELASQOL is a reliable and validated tool to measure the quality of life in Indians with melasma.


Assuntos
Melanose/diagnóstico , Melanose/etnologia , Qualidade de Vida , Inquéritos e Questionários , Tradução , Adolescente , Adulto , Fatores Etários , Estudos Transversais , Feminino , Humanos , Índia/etnologia , Idioma , Masculino , Melanose/psicologia , Medição de Risco , Fatores Sexuais , Adulto Jovem
15.
Virusdisease ; 27(3): 327-328, 2016 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-28466049

RESUMO

The occurrence of Pepper cryptic virus 2 was confirmed by RT-PCR and partial sequencing of coat protein gene in chilli cultivars Jwalamukhi and Jwalasakhi grown in the experimental farm at IARI, New Delhi. To the best of our knowledge this is the first report of occurrence of cryptovirus in chilli in India.

16.
Vet World ; 9(12): 1392-1399, 2016 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-28096611

RESUMO

Poultry is reared in open side houses in most of the tropical countries, which results in huge temperature variation in shed causing stress resulting in increased demand of antioxidant supplementation. Since cooling of poultry houses or environment control is very expensive, thus methods focused on nutritional modifications appears to be the much logical approach. Stress increases mineral and vitamin mobilization from tissues and their excretion. Effect of some minerals and vitamin supplements such as chromium (Cr) and ascorbic acid to elevate the negative effects of environmental stress is well documented. Cr functions as an antioxidant and its deficiency are said to disrupt carbohydrate and protein metabolism. Cr has been utilized for weight gain, to improve feed conversion ratio, increase relative organ weight, muscle development, decrease cholesterol, increase high-density lipoprotein cholesterol, and improve nutrient digestion. Therefore, the present review discusses the beneficial aspects of Cr with its effect in different doses and antioxidant combinations to explore and promote its optimum utilization in poultry nutrition and production.

17.
Indian J Otolaryngol Head Neck Surg ; 67(3): 242-7, 2015 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-26405658

RESUMO

We have described a new technique of using ultra-thin silicon sheet (0.2 mm) between two transected bony ends for temporo-mandibular joint (TMJ) ankylosis in children with advantages of short operative time, minimal foreign material insertion and faster recovery time post-operatively which makes our technique a good alternative to conventional techniques. Our study is a non-randomized prospective study conducted on 10 children aged between 4 and 15 years who presented to our tertiary care institute with severe trismus after traumatic injury and were willing to undergo this new technique. The main outcome measure taken into consideration was difference between pre-operative, intra-operative (on table) and post-operative mouth opening (minimum 2 years follow-up). The pre-operative mouth opening in our cases varied from 1 to 5 mm. The intra-operative mouth opening achieved ranged from 2.8 to 3.2 cm. The mouth opening was about more than 2.7 cm in all our cases at 2 years of follow-up. Our technique is a good alternative to conventional techniques used for TMJ ankylosis in children but few more randomized controlled trials are required to assess its effectiveness in comparison to conventional techniques and for universal adoption of this technique.

18.
J Food Sci Technol ; 52(8): 5075-83, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-26243928

RESUMO

Flaxseed imparted the evidence of health benefits in human being. Response surface methodology (RSM) was employed to develop flaxseed fortified rice - corn flour blend based extruded product using twin screw extruder. The effect of roasted flaxseed flour (RFF) fortification (15-25 %), moisture content of feed (12-16 %, wb), extruder barrel temperature (120-140 °C) and screw speed (300-330 RPM) on expansion ratio (ER), breaking strength (BS), bulk density (BD) and overall acceptability (OAA) score of extrudates were investigated using central composite rotatable design (CCRD). Increased RFF level decreased the ER and OAA score significantly while increased BS and BD of extrudates (p < 0.01). Moisture content of extruder feed was positively related to ER (p < 0.01) and OAA (p < 0.05) and negatively related to BD (p < 0.01). Extruder barrel temperature was found to be negatively related to ER and OAA (p < 0.05) and positively related to BD (p < 0.1). Quadratic effect of screw speed was significantly positively related to ER (p < 0.01), BS (p < 0.05) and negatively related to BD (p < 0.01). 15 % RFF fortification with rice flour, 16 % moisture content (wb) of extruder feed, 120 °C extruder barrel temperature and 330 RPM of screw speed gave an optimized product of high desirability with corresponding responses as 3.08 ER, 0.53 kgf BS, 0.106 g.cm(-3) BD and 7.86 OAA.

19.
Mol Biotechnol ; 57(7): 644-52, 2015 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-25854961

RESUMO

The present study was undertaken to clone and express the genes encoding antibody to the recombinant coat protein (rCP) of Papaya ringspot virus (PRSV) and to assess the engineered antibody for the detection of PRSV. A 33-kDa rCP of PRSV, which was produced in Escherichia coli, generated PRSV specific antibody in immunized mouse. The heavy and light chain variable domain genes (VH and VL) of 351 and 360 nucleotides, respectively, were cloned from the mRNA isolated from the spleen of the immunized mouse with rCP of PRSV. The VH and VL belong to the family IgG1 and kappa chain, respectively, and contained the framework regions and complementarity determining regions. The VH and VL genes were individually used to develop the expression constructs in pET28a (+) vector and 14-kDa proteins were obtained in E. coli. The amount of purified VH and VL proteins was 3-4 mg/l of bacterial culture. Both the antibody fragments recognized PRSV in the crude sap; however, the VL antibody fragment showed higher affinity to PRSV. The mixture of VH and VL detected PRSV as effectively as polyclonal antibody. The recombinant antibody fragments mixture detected PRSV in the field samples with 100 % accuracy in dot immunobinding assay (DIBA) and enzyme-linked immunosorbent assay (ELISA). The sensitivity of the detection of PRSV using antibody fragments was 1.0 and 10.0 ng in DIBA and ELISA, respectively. The results showed successful isolation of functional single-domain antibody encoding genes to PRSV directly from the immunized spleen cells of mouse. This study for the first time demonstrates application of bacterial expressed recombinant antibody fragments in immunodiagnosis of PRSV.


Assuntos
Anticorpos Monoclonais/imunologia , Proteínas do Capsídeo/imunologia , Testes Imunológicos , Potyvirus/isolamento & purificação , Animais , Proteínas do Capsídeo/genética , Carica/virologia , Escherichia coli/genética , Fragmentos de Imunoglobulinas/genética , Camundongos , Potyvirus/imunologia , Potyvirus/patogenicidade
20.
Arch Virol ; 160(5): 1297-301, 2015 May.
Artigo em Inglês | MEDLINE | ID: mdl-25698103

RESUMO

Few studies have been done on engineered antibodies for diagnosis of tospovirus infections. The present study was undertaken to develop a single-chain variable fragment (scFv) for specific diagnosis of infection by groundnut bud necrosis virus (GBNV), the most prevalent serogroup IV tospovirus in India. Heavy chain (372 nucleotide [nt]) and light chain (363 nt) variable region clones obtained from a hybridoma were used to make an scFv construct that expressed a ~29-kDa protein in E. coli. The scFv specifically detected GBNV in field samples of cowpea, groundnut, mung bean, and tomato, and it did not recognize watermelon bud necrosis virus, a close relative of GBNV belonging to tospovirus serogroup IV. This study for the first time demonstrated the application of a functional scFv against a serogroup-IV tospovirus.


Assuntos
Anticorpos Antivirais , Doenças das Plantas/virologia , Anticorpos de Cadeia Única , Tospovirus/isolamento & purificação , Anticorpos Antivirais/genética , Escherichia coli/genética , Fabaceae/virologia , Expressão Gênica , Testes Imunológicos/métodos , Índia , Lycopersicon esculentum/virologia , Proteínas Recombinantes/genética , Sensibilidade e Especificidade , Anticorpos de Cadeia Única/genética , Tospovirus/imunologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...