Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Mais filtros

Base de dados
Intervalo de ano de publicação
Plant Mol Biol ; 102(4-5): 417-430, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31898146


KEY MESSAGE: We genome-wide identified 28 JmjC domain-containing genes, further spatio-temporal expression profiling and genetic analysis defined them as epigenetic regulators in flowering initiation of Rosa chinensis. The JmjC domain-containing histone demethylases play critical roles in maintaining homeostasis of histone methylations, thus are vital for plant growth and development. Genome-wide identification of the JmjC domain-containing genes have been reported in several species, however, no systematic study has been performed in rose plants. In this paper, we identified 28 JmjC domain-containing genes from the newly published genome database of Rosa chinensis. The JmjC domain-containing proteins in R. chinensis were divided into seven groups, KDM3 was the largest group with 13 members, and JmjC domain-only A and KDM5B were the smallest clades both with only one member. Although all the JmjC domain proteins having a conserved JmjC domain, the gene and protein structure experienced differentiation and specification during the evolution, especially in KDM3 clade, one gene (RcJMJ40) was found carrying site deletions for cofactors Fe (II) and α-KG binding which were crucial for demethylase activities, three genes (RcJMJ41, RcJMJ43 and RcJMJ44) had no intron while two of them had tandem JmjC domains. Spatial expression pattern analysis of these JmjC domain-containing genes in different tissues showed most of them were highly expressed in reproductive tissues such as floral meristem and closed flowers than vegetative tissues, demonstrating their important functions in developmental switch from vegetative to reproductive growth of roses. Temporal expression profiling indicated majority of JmjC domain-containing genes from R. chinensis fluctuated along with floral bud differentiation and development, further proving their essential roles in flower organogenesis. VIGS induced silencing of RcJMJ12 led to delayed flowering time, and decreased the expression levels of flowering integrator such as RcFT, RcSOC1, RcFUL, RcLFY and RcAP1, therefore providing the genetic evidence of RcJMJ12 in flowering initiation. Collectively, spatio-temporal expression profiling and genetic analysis defined the JmjC domain-containing genes as important epigenetic regulators in flower development of R. chinensis.

Isotopes Environ Health Stud ; 54(1): 106-112, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-28915768


The 18O/16O and 2H/1H ratios of 18 water brands representing the most popular bottled water brands in the Saudi market were measured using a system based on the latest advancements in tunable off-axis integrated cavity output diode laser spectroscopy (OA-ICOS) in the near-infrared spectral region. Utilizing δ18O and the δ2H values of locally produced water samples, a meteoric water line (δ2H = 7.84 δ18O + 2.11) was extracted and found to be consistent with the slope of the global meteoric water line (GMWL) and the geographic location of Saudi Arabia.

Deutério/análise , Água Potável/análise , Isótopos de Oxigênio/análise , Oxigênio/análise , Arábia Saudita , Análise Espectral