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1.
J Small Anim Pract ; 2020 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-31960434

RESUMO

OBJECTIVES: To assess agreement between measurement of primary apocrine gland anal sac adenocarcinoma tumours using digital palpation, computed tomography (CT) and formalin-fixed tissue and to look for associations with metastasis at presentation. MATERIALS AND METHODS: Retrospective study of different methods of measuring primary tumour size in histopathologically-confirmed canine apocrine gland anal sac adenocarcinoma. RESULTS: One hundred sixteen tumours from 107 dogs were included. There was moderate agreement between maximal dimension of the primary tumour measured by CT compared to formalin-fixed tissue and digital palpation, respectively. There was no significant difference in median maximum dimension between the measurement methods. Vascular invasion, CT stage, digital rectal examination stage and formalin-fixed tissue stage were significantly associated with metastasis at presentation, while mitotic index of the primary tumour was not. Dogs with tumours >2.5 cm (tumour-stage 2) were significantly more likely to present with metastatic disease. CLINICAL SIGNIFICANCE: In canine apocrine gland anal sac adenocarcinoma, primary tumour size, tumour-stage and vascular invasion are strong predictors of metastasis at presentation.

2.
Ned Tijdschr Tandheelkd ; 126(12): 637-645, 2019 Dec.
Artigo em Holandês | MEDLINE | ID: mdl-31840674

RESUMO

Oral care for older people is an underexposed topic in dentistry as well as in general healthcare, while oral care professionals are increasingly confronted with frail and multimorbid older people with complex care needs. The research agenda 'Oral care for the elderly' was developed to encourage the collaboration of researchers in the Netherlands and Flanders (Belgium) to do more research in this area and in this way, to achieve an expansion and implementation of knowledge. This will make possible the provision of a socially responsible and robust basis for sustainable oral care for frail older people. The focus of the agenda is on 3 themes, namely oral health and oral function for older people; multi/interdisciplinary collaboration within primary care and the costs, benefits and long-term effect(s) of oral care throughout the entire course of life. This article provides an overview of this research agenda and the way in which it has been established.


Assuntos
Assistência à Saúde , Atenção Primária à Saúde , Idoso , Idoso de 80 Anos ou mais , Bélgica , Idoso Fragilizado , Humanos , Países Baixos , Saúde Bucal
3.
Ned Tijdschr Tandheelkd ; 126(12): 647-652, 2019 Dec.
Artigo em Holandês | MEDLINE | ID: mdl-31840675

RESUMO

The Netherlands, like other Western countries, shows an obvious demography of aging, which is associated with many challenges. People age differently, after all. Some remain vital until very old age, while others become frail and disabled much earlier in life. Because of the indicated demographic trend, morbidity is expected to increase and with it, the number of older people needing care. 'Aging in place' has become a central phenomenon in healthcare policies. This means that older people should be empowered to lead a meaningful life in their own living environment as long as possible, even when they are frail and care dependent. Therefore, in the future, most care for older people will actually occur at home. This article aims to present a meaningful care model for the older people. Starting from the 'definition discussion' about frailty and a revaluation of the concept of' resilience, a basis will be provided for a sustainable, proactive and personalised elderly care, close to the living environment of older people, in which dentists and other oral health professionals play an important role as well.


Assuntos
Pessoas com Deficiência , Idoso Fragilizado , Idoso , Idoso de 80 Anos ou mais , Odontólogos , Pessoal de Saúde , Humanos , Países Baixos
4.
Ned Tijdschr Tandheelkd ; 126(12): 657-664, 2019 Dec.
Artigo em Holandês | MEDLINE | ID: mdl-31840677

RESUMO

Current data show many older adults to have poor oral health, deteriorating even more when they become frail or care dependent. In order to achieve a structural and sustained improvement of this situation, it is necessary to have a clear view of the factors determining oral health in older adults, such as (endogenous (biological, lifestyle), environmental determinants and determinants associated with the organisation of healthcare. The various determinants show oral health in older adults is the result of thecombined effects of very diverse factors and for that reason, a solution will have to be based on a multidisciplinary approach, also involving people outside of clinical care. To promote good oral health over the full course of life, it is important to invest in a good start in life, that helps accumulate oral health gains, and at the same time it is essential to adapt the healthcare system and prevention strategies to the individual's needs in order to make oral care effective for the full course of life.


Assuntos
Assistência à Saúde , Saúde Bucal , Idoso , Humanos
5.
Ned Tijdschr Tandheelkd ; 126(12): 687-695, 2019 Dec.
Artigo em Holandês | MEDLINE | ID: mdl-31840681

RESUMO

Vurnerable older people often need intensive preventive and curative oral health care. However, their physical and cognitive decline does not always allow for lege artis implementation of dental treatments. This causes practical problems often with related moral issues. Including these moral issues in planning and implementation will make oral healthcare more effective and will also alleviate moral pressure on oral healthcare providers. Mostly the moral issues relate to every day ethics, which can be solved by all persons involved collectively, in open consultation and with an ethics of care approach. This means aiming at 'doing the right thing in the right way', including all the relevant aspects of the person of the patient and his/her environment. To achieve this, oral health care should be embedded in the overall care process for the elderly and care providers should feel involved with the older person and have reflective skills.


Assuntos
Tomada de Decisões , Idoso Fragilizado , Idoso , Idoso de 80 Anos ou mais , Assistência Odontológica , Feminino , Humanos , Masculino , Princípios Morais , Saúde Bucal
6.
Clin Oral Investig ; 22(1): 281-292, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-28367601

RESUMO

OBJECTIVES: The objective of the study was to evaluate the effect of an oral healthcare programme in nursing homes on care staff knowledge and attitude regarding oral health. METHODS: The study sample consisted of the nurses and nurses' aides from 63 nursing homes, which either received an oral healthcare programme including mobile dental care or were on a waiting list to receive this programme. A validated questionnaire completed at baseline and again after the study period assessed the care staff knowledge and attitude. Paired t test, independent t test, general linear and linear mixed models were used to examine the changes in attitude and knowledge scores. RESULTS: In total, 546 questionnaires were completed by the same people from 36 nursing homes at baseline and on completion of the study. After the intervention period, knowledge significantly improved in both study groups (I p < 0.001; C p < 0.001), the intervention group significantly showing the largest increase (p < 0.001). The outcome variable attitude only showed a significant improvement in the intervention group (p < 0.001). The mixed models confirmed the impact of some aspects of the intervention on the attitude and the knowledge of the caregivers. CONCLUSIONS: The oral healthcare programme including a mobile dental team resulted in a significant increase of the care staff knowledge and attitude regarding oral health. CLINICAL RELEVANCE: The integration of a dental professional team in nursing home organisations should be encouraged because it could be valuable to tackle barriers for the provision of daily oral hygiene and to support the continuous integration of oral health care into general care.


Assuntos
Assistência Odontológica para Idosos/organização & administração , Conhecimentos, Atitudes e Prática em Saúde , Unidades Móveis de Saúde , Enfermeiras e Enfermeiros/psicologia , Casas de Saúde , Saúde Bucal , Idoso , Bélgica , Feminino , Humanos , Masculino , Equipe de Assistência ao Paciente/organização & administração , Inquéritos e Questionários
7.
Community Dent Health ; 34(3): 143-151, 2017 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-28872808

RESUMO

OBJECTIVES: A study was conducted of nursing home residents with limited access to regular oral health care services to evaluate their oral health status, to perform an assessment of the need for oral treatment and to determine the possible predicting value of age, gender, care dependency and income level on their oral health status and treatment needs. MATERIALS AND METHODS: Three experienced dentists collected clinical oral health data with a mobile dental unit in 23 nursing homes. Socio-demographic data were extracted from the residents' records in the nursing home. Besides the descriptive and bivariate analysis, a general linear mixed model analysis was also performed with the nursing home as random effect. RESULTS: The study sample consisted of 1,226 residents with a mean age of 83.9 years, of which 41.9% were edentulous. The mean D3MFt in the dentate group was 24.5 and 77% needed extractions or fillings. In the group of residents wearing removable dentures, 36.9% needed repair, rebasing or renewal of the denture. The mixed model analysis demonstrated that with each year a resident gets older, the oral health outcomes get worse and that men have worse oral health and higher treatment needs than women. However, the level of income and care dependency had a less extensive role in predicting the oral health outcomes. CONCLUSIONS: The nursing home residents presented a poor overall oral health status and high dental and prosthetic treatment needs. Gender and age were important predicting variables for the oral health outcomes.


Assuntos
Determinação de Necessidades de Cuidados de Saúde , Casas de Saúde , Saúde Bucal , Idoso de 80 Anos ou mais , Bélgica , Feminino , Humanos , Masculino , Doenças da Boca
8.
Int J Dent Hyg ; 15(4): 306-312, 2017 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-27114100

RESUMO

OBJECTIVE: To assess the dental treatment backlog and associated costs among institutionalized elderly people using a novel composite index, called the oral health index. BACKGROUND: Despite numerous reports about oral health of old individuals, there is still lack of a systematic and practical method to estimate their treatment need covering all relevant aspects of oral health. In addition, little has been published on associated treatment costs and prediction of such costs. MATERIALS AND METHODS: An observational study was performed on 143 dentate institutionalized elderly people, whereby several clinical parameters were registered. The collected data were included in the oral health index representing the need for oral health care. This covered the number of caries lesions, number of residual roots, periodontal health condition, plaque score and denture condition with a final score of 0-9. To investigate the validity of the index, the treatment costs were estimated using the measured clinical parameters and later compared with the actual expenditure of the patients for the following 2 years. RESULTS: The average score of the index was 4.6 (SD 1.4) with 65.1% of the individuals having a score between 3 and 6 and 27.3% having a score of 6 and more, exhibiting medium and high need for oral health care, respectively. Only 30% of the patients underwent all the indicated treatments. The major reason of non-completion of the treatment was patient's refusal. From the fully treated group, 61.5% of the subjects actually spent within the predicted range while 38.5% of them spent more than estimated. The underestimation was related to yearly calculus removals leading to repeated calculation of the same costs and newly emerging dental problems (33% developed new caries and 20% was confronted with tooth fracture within the 2-year period). CONCLUSION: The novel index can be helpful to determine oral treatment needs and associated costs. Further research is needed to extend the clinical applicability of the index.


Assuntos
Necessidades e Demandas de Serviços de Saúde , Serviços de Saúde para Idosos/economia , Institucionalização , Saúde Bucal , Idoso , Bélgica , Estudos Transversais , Feminino , Gastos em Saúde , Humanos , Masculino , Projetos Piloto
9.
Vet Rec ; 179(1): 17, 2016 Jul 02.
Artigo em Inglês | MEDLINE | ID: mdl-27302919

RESUMO

Patent ductus arteriosus (PDA) is infrequently reported in cats and represents between 1-7.3 per cent of left to right shunting cardiac congenital anomalies. The objective of this study was to report the presenting complaints, clinical examination findings, diagnostic findings, treatment outcomes and survival times in cats diagnosed with a PDA in the UK. Medical records from three major UK referral centres were searched for cats that were diagnosed with PDA from January 2004 to December 2012. Data obtained for analysis included: signalment, clinical examination findings including murmur characteristics, diagnostic imaging findings, treatment outcomes and survival times. Nineteen cats were included in the analysis. The most common reason for referral was investigation of an incidentally detected heart murmur without clinical signs (13/19; 68 per cent). Pulmonary arterial hypertension (PAH) was diagnosed in seven (37 per cent) cats and those cats with PAH were significantly more likely to present with signs of disease (P=0.004). Median survival time in cats that were diagnosed with PDA and died due to cardiac causes was 898 days (interquartile range 459-1011 days). The median survival time of those cats that had an additional congenital anomaly was significantly shorter to those cats without a congenital anomaly (P=0.008).


Assuntos
Doenças do Gato/diagnóstico , Doenças do Gato/terapia , Permeabilidade do Canal Arterial/veterinária , Animais , Gatos , Permeabilidade do Canal Arterial/diagnóstico , Permeabilidade do Canal Arterial/terapia , Feminino , Masculino , Estudos Retrospectivos , Resultado do Tratamento , Reino Unido
10.
Vet Rec ; 175(11): 280, 2014 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-24859353

RESUMO

NSAIDs are the cornerstone of medical management of canine osteoarthritis (OA). Meloxicam is a daily-administered NSAID widely available in a liquid formulation and manufacturer's summary of product characteristics (SPC) advise that it is given at the lowest effective dose. Mavacoxib is a long-acting NSAID given as a monthly tablet. This study compares these drugs in the management of canine OA. In all, 111 dogs with OA of the elbow, hip or stifle were randomly assigned to receive one of these NSAIDs for a 12-week period, and to administer them as per the manufacturer's SPC. Outcomes, including ground reaction forces and three validated clinical metrology instruments, were measured at baseline, 6 and 12 weeks. Improvements were seen in all outcome measures for both groups to a similar degree, and adverse events occurred at a similar rate. There were significant improvements in outcome measures from week 6 to week 12, as well as from baseline. Long-term meloxicam dose was more important than recent dose. Clinical efficacy and adverse event rates are similar for meloxicam and mavacoxib when administered as per their UK SPC. This is relevant information for veterinary surgeons when prescribing NSAID treatment for canine OA.


Assuntos
Anti-Inflamatórios não Esteroides/uso terapêutico , Doenças do Cão/tratamento farmacológico , Osteoartrite/veterinária , Pirazóis/uso terapêutico , Tiazinas/uso terapêutico , Tiazóis/uso terapêutico , Animais , Cães , Feminino , Masculino , Meloxicam , Osteoartrite/tratamento farmacológico , Resultado do Tratamento
11.
Acta Clin Belg ; 68(6): 416-20, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24635328

RESUMO

Tetanus is a very serious neuromuscular disease caused by a powerful exotoxin, tetanospasmin, from the Clostridium tetani bacillus. Its incidence in the developed world has diminished considerably since the introduction of primary vaccination. Tetanus is diagnosed clinically, through recognition of the characteristically inducible muscle spasms. Three clinical forms described in adults are generalised, localised and cephalic tetanus. Management of tetanus aims at removing the source of tetanospasmin, neutralising circulating toxin, and providing adequate supportive care for muscle spasms, respiration and autonomic instability. Tetanus is a forgotten disease in developed countries since many practicing primary care physicians have not seen a single case in their career. We present a case of tetanus and review briefly the pathogenesis, clinical features and therapy in order to educate the internist in recognising and adequately treating this disease.


Assuntos
Antibacterianos/administração & dosagem , Emergências , Fatores Imunológicos/administração & dosagem , Antitoxina Tetânica/administração & dosagem , Tétano/diagnóstico , Tétano/terapia , Idoso , Astenia/microbiologia , Feminino , Humanos , Respiração Artificial/métodos , Tétano/microbiologia , Resultado do Tratamento , Trismo/microbiologia , Ocidente
12.
Trans R Soc Trop Med Hyg ; 103(9): 949-51, 2009 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-19278704

RESUMO

An outbreak investigation was conducted during February-March 2005 to determine the cause of several sudden deaths occurring in Pailin Province, Cambodia. Sixty-seven patients presented with non-febrile poisoning-like symptoms and 15 died of coma, including 53% children under 10 years old. Symptoms included sore throat (92%), sore lips (73%), swollen tongue (54%) and gastrointestinal signs (41%). A plant locally called prik was the source of poisoning (97.0 vs. 28.7%, odds ratio 74.3, P<0.001). Patients may have confused the edible Melientha suavis Pierre with Urobotrya siamensis Hiepko, both from the Opiliaceae family. This was the first report of Urobotrya poisoning and its clinical manifestations.


Assuntos
Intoxicação por Plantas/epidemiologia , Adolescente , Adulto , Camboja/epidemiologia , Criança , Pré-Escolar , Coma/etiologia , Morte Súbita/etiologia , Surtos de Doenças , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Intoxicação por Plantas/mortalidade , Intoxicação por Plantas/fisiopatologia , Adulto Jovem
13.
Bull World Health Organ ; 85(11): 880-5, 2007 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-18038079

RESUMO

PROBLEM: In Cambodia, care for people with HIV/AIDS (prevalence 1.9%) is expanding, but care for people with type II diabetes (prevalence 5-10%), arterial hypertension and other treatable chronic diseases remains very limited. APPROACH: We describe the experience and outcomes of offering integrated care for HIV/AIDS, diabetes and hypertension within the setting of chronic disease clinics. LOCAL SETTING: Chronic disease clinics were set up in the provincial referral hospitals of Siem Reap and Takeo, 2 provincial capitals in Cambodia. RELEVANT CHANGES: At 24 months of care, 87.7% of all HIV/AIDS patients were alive and in active follow-up. For diabetes patients, this proportion was 71%. Of the HIV/AIDS patients, 9.3% had died and 3% were lost to follow-up, while for diabetes this included 3 (0.1%) deaths and 28.9% lost to follow-up. Of all diabetes patients who stayed more than 3 months in the cohort, 90% were still in follow-up at 24 months. LESSONS LEARNED: Over the first three years, the chronic disease clinics have demonstrated the feasibility of integrating care for HIV/AIDS with non-communicable chronic diseases in Cambodia. Adherence support strategies proved to be complementary, resulting in good outcomes. Services were well accepted by patients, and this has had a positive effect on HIV/AIDS-related stigma. This experience shows how care for HIV/AIDS patients can act as an impetus to tackle other common chronic diseases.


Assuntos
Instituições de Assistência Ambulatorial/organização & administração , Doença Crônica/terapia , Diabetes Mellitus Tipo 2/terapia , Infecções por HIV/terapia , Hipertensão/terapia , Camboja/epidemiologia , Infecções por HIV/mortalidade , Humanos , Resultado do Tratamento
14.
Br J Cancer ; 97(10): 1344-53, 2007 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-18000499

RESUMO

R306465 is a novel hydroxamate-based histone deacetylase (HDAC) inhibitor with broad-spectrum antitumour activity against solid and haematological malignancies in preclinical models. R306465 was found to be a potent inhibitor of HDAC1 and -8 (class I) in vitro. It rapidly induced histone 3 (H3) acetylation and strongly upregulated expression of p21waf1,cip1, a downstream component of HDAC1 signalling, in A2780 ovarian carcinoma cells. R306465 showed class I HDAC isotype selectivity as evidenced by poor inhibition of HDAC6 (class IIb) confirmed by the absence of downregulation of Hsp90 chaperone c-raf protein expression and tubulin acetylation. This distinguished it from other HDAC inhibitors currently in clinical development that were either more potent towards HDAC6 (e.g. vorinostat) or had a broader HDAC inhibition spectrum (e.g. panobinostat). R306465 potently inhibited cell proliferation of all main solid tumour indications, including ovarian, lung, colon, breast and prostate cancer cell lines, with IC50 values ranging from 30 to 300 nM. Haematological cell lines, including acute lymphoblastic leukaemia, acute myeloid leukaemia, chronic lymphoblastic leukaemia, chronic myeloid leukaemia, lymphoma and myeloma, were potently inhibited at a similar concentration range. R306465 induced apoptosis and inhibited angiogenesis in cell-based assays and had potent oral in vivo antitumoral activity in xenograft models. Once-daily oral administration of R306465 at well-tolerated doses inhibited the growth of A2780 ovarian, H460 lung and HCT116 colon carcinomas in immunodeficient mice. The high activity of R306465 in cell-based assays and in vivo after oral administration makes R306465 a promising novel antitumoral agent with potential applicability in a broad spectrum of human malignancies.


Assuntos
Antineoplásicos/administração & dosagem , Antineoplásicos/farmacologia , Inibidores Enzimáticos/administração & dosagem , Inibidores Enzimáticos/farmacologia , Inibidores de Histona Desacetilases , Ácidos Hidroxâmicos/farmacologia , Neoplasias/tratamento farmacológico , Sulfonas/farmacologia , Administração Oral , Inibidores da Angiogênese/administração & dosagem , Inibidores da Angiogênese/química , Inibidores da Angiogênese/farmacologia , Animais , Antineoplásicos/química , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Inibidor de Quinase Dependente de Ciclina p21/efeitos dos fármacos , Inibidor de Quinase Dependente de Ciclina p21/genética , Modelos Animais de Doenças , Relação Dose-Resposta a Droga , Ativação Enzimática/efeitos dos fármacos , Inibidores Enzimáticos/química , Histonas/efeitos dos fármacos , Histonas/metabolismo , Humanos , Ácidos Hidroxâmicos/química , Imuno-Histoquímica , Isoenzimas/antagonistas & inibidores , Masculino , Camundongos , Camundongos Nus , Neoplasias/patologia , Regiões Promotoras Genéticas/efeitos dos fármacos , Regiões Promotoras Genéticas/genética , Ratos , Ratos Sprague-Dawley , Sulfonas/química , Células Tumorais Cultivadas , Ensaios Antitumorais Modelo de Xenoenxerto
15.
Trop Med Int Health ; 12(2): 251-9, 2007 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-17300633

RESUMO

OBJECTIVES: To compare the efficacy and tolerability of dihydroartemisinin-piperaquine (DHA-PQP) with that of a 3-day regimen of mefloquine and artesunate (MAS3) for the treatment of uncomplicated falciparum malaria in Cambodia. METHOD: Randomized open-label non-inferiority study over 64 days. RESULTS: Four hundred and sixty-four patients were included in the study. The polymerase chain reaction genotyping-adjusted cure rates on day 63 were 97.5% (95% confidence interval, CI, 93.8-99.3) for DHA-PQP and 97.5% (95% CI, 93.8-99.3) for MAS3, P = 1. There were no serious adverse events, but significantly more episodes of vomiting (P = 0.03), dizziness (P = 0.002), palpitations (P = 0.04), and sleep disorders (P = 0.03) reported in the MAS3 treatment group, consistent with the side-effect profile of mefloquine. CONCLUSIONS: DHA-PQP was as efficacious as MAS3, but much better tolerated, making it more appropriate for use in a routine programme setting. This highly efficacious, safe and more affordable fixed-dose combination could become the treatment of choice for Plasmodium falciparum malaria in Cambodia.


Assuntos
Antimaláricos/uso terapêutico , Artemisininas/uso terapêutico , Malária Falciparum/tratamento farmacológico , Quinolinas/uso terapêutico , Sesquiterpenos/uso terapêutico , Adolescente , Adulto , Idoso , Anemia/complicações , Anemia/epidemiologia , Antimaláricos/efeitos adversos , Artemisininas/efeitos adversos , Artesunato , Camboja/epidemiologia , Criança , Pré-Escolar , Quimioterapia Combinada , Feminino , Genoma de Protozoário , Humanos , Lactente , Malária Falciparum/epidemiologia , Malária Falciparum/parasitologia , Malária Vivax/tratamento farmacológico , Malária Vivax/epidemiologia , Malária Vivax/parasitologia , Masculino , Mefloquina/uso terapêutico , Pessoa de Meia-Idade , Quinolinas/efeitos adversos , Recidiva , Sesquiterpenos/efeitos adversos , Resultado do Tratamento
16.
J Cell Sci ; 114(Pt 17): 3177-88, 2001 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-11590244

RESUMO

Cadherins are major cell-cell adhesion proteins whose cytoplasmic domains bind to catenin proteins. Strong intercellular adhesion depends on linkage of the cadherin/catenin complex to the actin cytoskeleton via alpha-catenin. To date, it is not clear how different cell types achieve the variable strength of cell-cell adhesion clearly needed in a multicellular organism. Here, we report the cloning and molecular characterization of alphaT(testis)-catenin, a novel human cDNA encoding a protein with homology to both human alphaE(epithelial)-catenin and alphaN(neural)-catenin. Although originally discovered in testis, alphaT-catenin is expressed in other tissues, the highest levels being observed in heart. Immunohistochemical analysis showed human alphaT-catenin localization at intercalated discs of cardiomyocytes and in peritubular myoid cells of testis. In cells transfected with alphaT-catenin cDNA, interaction with beta-catenin was demonstrated by co-immunoprecipitation. Transfection of alpha-catenin-deficient colon carcinoma cells recruited E-cadherin and beta-catenin to cell-cell contacts and functional cadherin-mediated cell-cell adhesion was restored in this way. Moreover, compaction of these cells was at least as prominent as in the case of cells expressing endogenous alphaE-catenin. We propose that alphaT-catenin is necessary for the formation of stretch-resistant cell-cell adhesion complexes, in particular, muscle cells.


Assuntos
Caderinas/metabolismo , Caderinas/fisiologia , Proteínas do Citoesqueleto/química , Proteínas do Citoesqueleto/metabolismo , Proteínas do Citoesqueleto/fisiologia , Transativadores , Sequência de Aminoácidos , Animais , Western Blotting , Adesão Celular , Comunicação Celular , Linhagem Celular , Células Cultivadas , Clonagem Molecular , DNA Complementar/metabolismo , Eletroforese em Gel de Poliacrilamida , Proteínas de Fluorescência Verde , Humanos , Imuno-Histoquímica , Proteínas Luminescentes/metabolismo , Masculino , Camundongos , Microscopia de Fluorescência , Dados de Sequência Molecular , Músculos/citologia , Miocárdio/citologia , Miocárdio/metabolismo , Plasmídeos/metabolismo , Testes de Precipitina , Ligação Proteica , Estrutura Terciária de Proteína , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Homologia de Sequência de Aminoácidos , Testículo/citologia , Testículo/metabolismo , Distribuição Tecidual , Transfecção , Células Tumorais Cultivadas , Vinculina/metabolismo , alfa Catenina , beta Catenina
17.
J Appl Microbiol ; 91(3): 470-7, 2001 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-11556912

RESUMO

AIMS: To determine optimal exopolysaccharide (EPS) production conditions of the mesophilic lactic acid bacterium strain Lactobacillus sakei 0-1 and to detect possible links between EPS yields and the activity of relevant enzymes. METHODS AND RESULTS: Fermentation experiments at different temperatures using either glucose or lactose were carried out. EPS production took place during the exponential growth phase. Low temperatures, applying glucose as carbohydrate source, resulted in the best bacterial growth, the highest amounts of EPS and the highest specific EPS production. Activities of 10 important enzymes involved in the EPS biosynthesis and the energy formation of Lact. sakei 0-1 were measured. The obtained results revealed that there is a clear link for some enzymes with EPS biosynthesis. It was also demonstrated clearly that the presence of rhamnose in the EPS building blocks is due to high activities of the enzymes involved in the rhamnose synthetic branch. CONCLUSION: EPS production in Lact. sakei 0-1 is growth-associated and displays primary metabolite kinetics. Glucose as carbohydrate source and low temperatures enhance the EPS production. The enzymes involved in the biosynthesis of the activated sugar nucleotides play a major role in determining the monomeric composition of the synthesized EPS. SIGNIFICANCE AND IMPACT OF THE STUDY: The proposed results contribute to a better understanding of the physiological factors influencing EPS production and the key enzymes involved in EPS biosynthesis by Lact. sakei.


Assuntos
Glucose/metabolismo , Lactobacillus/enzimologia , Lactobacillus/metabolismo , Lactose/metabolismo , Polissacarídeos Bacterianos/biossíntese , Cápsulas Bacterianas/biossíntese , Contagem de Colônia Microbiana , Meios de Cultura , Fermentação , Cinética , Lactobacillus/crescimento & desenvolvimento , Ramnose/biossíntese , Temperatura Ambiente
18.
J Exp Biol ; 203(Pt 24): 3717-25, 2000 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-11076735

RESUMO

Oxygen, while being an obligate fuel for aerobic life, has been shown to be toxic through its deleterious reactive species, which can cause oxidative stress and lead ultimately to cell and organism death. In marine organisms, reactive oxygen species (ROS), such as the superoxide anion and hydrogen peroxide, are generated within respiring cells and tissues and also by photochemical processes in sea water. Considering both the reduced metabolic rate of nektonic organisms thriving in the deep sea and the physico-chemical conditions of this dark, poorly oxygenated environment, the meso- and bathypelagic waters of the oceans might be considered as refuges against oxidative dangers. This hypothesis prompted us to investigate the activities of the three essential enzymes (superoxide dismutase, SOD; catalase, CAT; glutathione peroxidase, GPX) constitutive of the antioxidative arsenal of cells in the tissues of 16 species of meso- and bathypelagic fishes occurring between the surface and a depth of 1300 m. While enzymatic activities were detected in all tissues from all species, the levels of SOD and GPX decreased in parallel with the exponential reduction in the metabolic activity as estimated by citrate synthase activity. In contrast, CAT was affected neither by the metabolic activity nor by the depth of occurrence of the fishes. High levels of metabolic and antioxidative enzymes were detected in the light organs of bioluminescent species. The adjustment of the activity of SOD and GPX to the decreased metabolic activity associated with deep-sea living suggests that these antioxidative defense mechanisms are used primarily against metabolically produced ROS, whereas the maintenance of CAT activity throughout all depths could be indicative of another role. The possible reasons for the occurrence of such a reduced antioxidative arsenal in deep-sea species are discussed.


Assuntos
Antioxidantes/metabolismo , Catalase/metabolismo , Peixes/metabolismo , Glutationa Peroxidase/metabolismo , Superóxido Dismutase/metabolismo , Anaerobiose , Animais , Citrato (si)-Sintase/metabolismo , Peixes/classificação , Medições Luminescentes , Estresse Oxidativo , Oxigênio/metabolismo , Água do Mar
19.
Biochim Biophys Acta ; 1447(2-3): 341-7, 1999 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-10542337

RESUMO

A new human cDNA was cloned and termed alpha-catulin, based on sequence similarity with both alpha-CATenins and vincULIN. The mRNA is present ubiquitously, although low expression levels are found in neural tissues. The genomic organization of the alpha-catulin gene CTNNAL1 is closely related to that of the alphaE-catenin gene CTNNA1, but not at all to that of the vinculin gene. Alternative splicing of the last exon generates a frameshift, resulting in a truncated protein with a new carboxy-terminus.


Assuntos
Proteínas do Citoesqueleto/genética , DNA Complementar/genética , Genoma Humano , Processamento Alternativo , Sequência de Aminoácidos , Clonagem Molecular , Sequência Conservada , Humanos , Dados de Sequência Molecular , Alinhamento de Sequência , Análise de Sequência , alfa Catenina
20.
J Exp Biol ; 201(Pt 8): 1211-21, 1998 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-9510532

RESUMO

Bioluminescence, the emission of ecologically functional light by living organisms, emerged independently on several occasions, yet the evolutionary origins of most bioluminescent systems remain obscure. We propose that the luminescent substrates of the luminous reactions (luciferins) are the evolutionary core of most systems, while luciferases, the enzymes catalysing the photogenic oxidation of the luciferin, serve to optimise the expression of the endogenous chemiluminescent properties of the luciferin. Coelenterazine, a luciferin occurring in many marine bioluminescent groups, has strong antioxidative properties as it is highly reactive with reactive oxygen species such as the superoxide anion or peroxides. We suggest that the primary function of coelenterazine was originally the detoxification of the deleterious oxygen derivatives. The functional shift from its antioxidative to its light-emitting function might have occurred when the strength of selection for antioxidative defence mechanisms decreased. This might have been made possible when marine organisms began colonising deeper layers of the oceans, where exposure to oxidative stress is considerably reduced because of reduced light irradiance and lower oxygen levels. A reduction in metabolic activity with increasing depth would also have decreased the endogenous production of reactive oxygen species. Therefore, in these organisms, mechanisms for harnessing the chemiluminescence of coelenterazine in specialised organs could have developed, while the beneficial antioxidative properties were maintained in other tissues. The full range of graded irradiance in the mesopelagic zone, where the majority of organisms are bioluminescent, would have provided a continuum for the selection and improvement of proto-bioluminescence. Although the requirement for oxygen or reactive oxygen species observed in bioluminescent systems reflects the high energy required to produce visible light, it may suggest that oxygen-detoxifying mechanisms provided excellent foundations for the emergence of many bioluminescent systems.


Assuntos
Adaptação Fisiológica/fisiologia , Comunicação Animal , Evolução Biológica , Medições Luminescentes , Oxigênio/metabolismo , Oxigênio/fisiologia , Animais
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