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1.
Exp Ther Med ; 22(6): 1357, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34659503

RESUMO

Picrasma quassioides (D. Don) Benn is an Asian shrub with a considerable history of traditional medicinal use. P. quassioides and its extracts exhibit good therapeutic properties against several diseases, including anti-inflammatory, antibacterial and anticancer effects. However, the composition of compounds contained in P. quassioides is complex; although various studies have examined mixtures or individual compounds extracted from it, studies on the application of P. quassioides extracts remain limited. In the present review, the structures and functions of the compounds identified from P. quassioides and their utility in anti-inflammatory, anticancer and neuroprotectant therapies was discussed. The present review provided up-to-date information on pharmacological activities and clinical applications for P. quassioides extracts.

2.
In Vivo ; 35(5): 2599-2608, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34410947

RESUMO

BACKGROUND/AIM: Asian Traditional medicines are renowned for their antitumor properties and are efficacious in the clinical treatment of various cancer types. ERM210 is a Korean traditional medicine comprising nine types of medicinal plants. In the present study, we examined the pro-apoptotic effect and molecular mechanisms of the effects of ERM210 on HepG2 liver cancer cells. MATERIALS AND METHODS: The cytotoxicity of ERM210 on HepG2 cells was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and wound-healing assays, and apoptosis and signaling pathways by fluorescence microscopy flow cytometry and western blotting. RESULTS: ERM210 significantly impaired HepG2 cell viability and enhanced mitochondria-dependent cellular apoptosis in a time- and dose-dependent manner by up-regulating the expression of caspases 3, 7 and 9, and of BCL2 apoptosis regulator (BCL2)-associated X, apoptosis regulator (BAX) proteins, whilst down-regulating that of BCL2 protein. Furthermore, ERM210 treatment increased accumulation of cellular and mitochondrial reactive oxygen species (ROS) and significantly inhibited cell migration. Additionally, all these phenomena were reversed by treating with the ROS scavenger N-acetylcysteine. The analysis of signaling proteins revealed that ERM210 significantly up-regulated the phosphorylation of ROS-dependent mitogen-activated protein kinases (p38, extracellular-regulated kinase, and c-Jun N-terminal kinase in HepG2 liver cancer cells. CONCLUSION: ERM210 exerts anticancer effects in HepG2 liver cancer cells by up-regulating ROS/mitochondria-dependent apoptosis signaling, providing new insight into the possibility of employing this traditional medicine for the clinical treatment of liver cancer.


Assuntos
Apoptose , Neoplasias Hepáticas , Células Hep G2 , Humanos , Neoplasias Hepáticas/tratamento farmacológico , Neoplasias Hepáticas/genética , Neoplasias Hepáticas/metabolismo , Potencial da Membrana Mitocondrial , Mitocôndrias/metabolismo , Espécies Reativas de Oxigênio/metabolismo
3.
ACS Appl Mater Interfaces ; 12(23): 26313-26319, 2020 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-32400150

RESUMO

Dynamic surface modification of suspended graphene at high temperatures was directly observed with in situ scanning transmission electron microscopy (STEM) measurements. The suspended graphene devices were prepared on a SiN membrane substrate with a hole so that STEM observations could be conducted during Joule heating. Current-voltage characteristics of suspended graphene devices inside the STEM chamber were measured while monitoring and controlling the temperature of graphene by estimating the electrical power of the devices. During the in situ STEM observation at high temperatures, residual hydrocarbon adsorbents that had remained on graphene effectively evaporated creating large, atomically clean graphene areas. At other places, dynamic changes in the shape, position, and orientation of adsorbents could be directly observed. The temperature of the suspended graphene sample was estimated to reach up to 2000 K during the experiment, making graphene an efficient high-temperature micrometer-sized electron-transparent hot plate for future experiments in microscopes.

4.
PLoS Genet ; 10(8): e1004545, 2014 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-25121504

RESUMO

Transcriptional/translational feedback loops drive daily cycles of expression in clock genes and clock-controlled genes, which ultimately underlie many of the overt circadian rhythms manifested by organisms. Moreover, phosphorylation of clock proteins plays crucial roles in the temporal regulation of clock protein activity, stability and subcellular localization. dCLOCK (dCLK), the master transcription factor driving cyclical gene expression and the rate-limiting component in the Drosophila circadian clock, undergoes daily changes in phosphorylation. However, the physiological role of dCLK phosphorylation is not clear. Using a Drosophila tissue culture system, we identified multiple phosphorylation sites on dCLK. Expression of a mutated version of dCLK where all the mapped phospho-sites were switched to alanine (dCLK-15A) rescues the arrythmicity of Clk(out) flies, yet with an approximately 1.5 hr shorter period. The dCLK-15A protein attains substantially higher levels in flies compared to the control situation, and also appears to have enhanced transcriptional activity, consistent with the observed higher peak values and amplitudes in the mRNA rhythms of several core clock genes. Surprisingly, the clock-controlled daily activity rhythm in dCLK-15A expressing flies does not synchronize properly to daily temperature cycles, although there is no defect in aligning to light/dark cycles. Our findings suggest a novel role for clock protein phosphorylation in governing the relative strengths of entraining modalities by adjusting the dynamics of circadian gene expression.


Assuntos
Proteínas CLOCK/genética , Ritmo Circadiano/genética , Proteínas de Drosophila/genética , Drosophila melanogaster/genética , Alanina/genética , Animais , Proteínas CLOCK/biossíntese , Proteínas de Drosophila/biossíntese , Drosophila melanogaster/crescimento & desenvolvimento , Regulação da Expressão Gênica no Desenvolvimento , Mutação , Fosforilação/genética , RNA Mensageiro/biossíntese
5.
Appl Microbiol Biotechnol ; 98(20): 8629-39, 2014 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-24993358

RESUMO

Isochrysis galbana and Isochrysis sp. are economically important microalgae from the division of haptophytes. Here, we report Agrobacterium-mediated stable DNA transfer into their nuclear genomes. Initial studies were performed to standardize co-cultivation media and determine the sensitivity of the microalgae to selective agents. Up to 1 mg/ml of the antibiotic hygromycin did not inhibit growth, whereas both the haptophytes bleached in artificial seawater (ASW) medium containing micromolar concentrations of the herbicide norflurazon. Co-cultivation of Isochrysis sp. and I. galbana with Agrobacterium tumefaciens strain LBA 4404 harboring the binary vector pCAMBIA 1380-pds-L504R yielded norflurazon-resistant (NR) colonies visible on selective plates after 20-30 days. pCAMBIA 1380-pds-L540R was constructed by cloning a mutated genomic phytoene desaturase (pds) gene from Haematococcus pluvialis as a selectable marker gene into the binary vector system pCAMBIA 1380. Co-cultivation of Isochrysis sp. with A. tumefaciens in ASW medium containing 200 µM of acetosyringone for 72 h produced the highest number of NR cells. For I. galbana, 100 µM of acetosyringone, ASW medium, and 48 h co-cultivation period appeared to be optimum co-cultivation parameters. The NR colonies kept their resistance phenotype for at least 24 months, even in the absence of selective pressure. The transfer of the pds gene in NR cells was shown by PCR amplification of the T-DNA sequences from the genomic DNA of NR cells and Southern blot analysis using T-DNA sequences as probes. The genetic manipulation described here will allow metabolic engineering and a better understanding of several biochemical pathways in the future.


Assuntos
Agrobacterium tumefaciens/genética , Vetores Genéticos , Genética Microbiana/métodos , Haptófitas/genética , Biologia Molecular/métodos , Transformação Genética , Anti-Infecciosos/metabolismo , Cinamatos/metabolismo , Meios de Cultura/química , Higromicina B/análogos & derivados , Higromicina B/metabolismo , Piridazinas/metabolismo , Seleção Genética , Fatores de Tempo
6.
J Vet Sci ; 13(4): 429-32, 2012 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-23271186

RESUMO

Monoclonal antibodies (mAbs) specific for the abnormal prion protein isoform (PrP(res)) are indispensable for diagnosing chronic wasting disease (CWD). In this study, eight mAbs were developed by immunizing PrP knockout mice with recombinant elk PrP and an immunogenic PrP peptide. The reactivity of the mAbs to recombinant PrP and the PrP peptide was measured, and their isotypes were subsequently determined. Among them, four mAbs (B85-05, B85-08, B85-12, and B77-75) were shown by Western blotting to recognize proteinase K-treated brain homogenate derived from an elk suffering from CWD.


Assuntos
Anticorpos Monoclonais , Cervos/imunologia , Cervos/metabolismo , Príons/imunologia , Isoformas de Proteínas/imunologia , Doença de Emaciação Crônica/diagnóstico , Doença de Emaciação Crônica/imunologia , Animais , Anticorpos Monoclonais/genética , Western Blotting/veterinária , Primers do DNA/genética , Eletroforese em Gel de Poliacrilamida/veterinária , Endopeptidase K , Camundongos , Camundongos Knockout , Reação em Cadeia da Polimerase/veterinária , Príons/genética , Isoformas de Proteínas/genética
7.
Genes Dev ; 26(5): 490-502, 2012 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-22327476

RESUMO

Post-translational modifications of one or more central "clock" proteins, most notably time-of-day-dependent changes in phosphorylation, are critical for setting the pace of circadian (≅24 h) clocks. In animals, PERIOD (PER) proteins are the key state variable regulating circadian clock speed and undergo daily changes in abundance and cytoplasmic-nuclear distribution that are partly driven by a complex phosphorylation program. Here, we identify O-GlcNAcylation (O-GlcNAc) as a critical post-translational modification in circadian regulation that also contributes to setting clock speed. Knockdown or overexpression of Drosophila O-GlcNAc transferase (ogt) in clock cells either shortens or lengthens circadian behavioral rhythms, respectively. The Drosophila PERIOD protein (dPER) is a direct target of OGT and undergoes daily changes in O-GlcNAcylation, a modification that is mainly observed during the first half of the night, when dPER is predominantly located in the cytoplasm. Intriguingly, the timing of when dPER translocates from the cytoplasm to the nucleus is advanced or delayed in flies, wherein ogt expression is reduced or increased, respectively. Our results suggest that O-GlcNAcylation of dPER contributes to setting the correct pace of the clock by delaying the timing of dPER nuclear entry. In addition, OGT stabilizes dPER, suggesting that O-GlcNAcylation has multiple roles in circadian timing systems.


Assuntos
Relógios Circadianos/fisiologia , Drosophila melanogaster/fisiologia , Acilação , Animais , Caseína Quinase Iépsilon/metabolismo , Linhagem Celular , Núcleo Celular/metabolismo , Células Cultivadas , Proteínas de Drosophila/metabolismo , Drosophila melanogaster/metabolismo , Regulação Enzimológica da Expressão Gênica , Técnicas de Silenciamento de Genes , N-Acetilglucosaminiltransferases/metabolismo , Neurônios/enzimologia , Neurônios/metabolismo , Proteínas Circadianas Period/metabolismo , RNA Mensageiro/metabolismo
8.
Curr Microbiol ; 63(2): 213-7, 2011 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-21674165

RESUMO

A Gram-negative, non-motile, catalase- and oxidase- positive, strictly aerobic, and short rod-shaped bacterium that was designated strain KOPRI 25157(T) was isolated from coastal seawater sample in Antarctica. The temperature and pH ranges for growth on R2A agar were 10-20°C, and 5.0-10.0, respectively. Phylogenetic analyses of the 16S rRNA gene sequence of strain KOPRI 25157(T) showed it to belong to the family Oxalobacteraceae of the class Betaproteobacteria, and it formed a distinct clade from other recognized members of the family. DNA G + C content was 65.9 mol%. Major ubiquinone was Q-8. Predominant cellular fatty acids were C(16:1) ω7c/15 iso 2OH (56.4%) and C(16:1) (30.5%). Major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, and unknown lipid. On the basis of these data, it is proposed that strain KOPRI 25157(T) is the representative of a novel genus, for which the name Actimicrobium gen. nov. is proposed in the family Oxalobacteraceae. The type strain for Actimicrobium antarcticum sp. nov. is KOPRI 25157(T) (=JCM 16673(T)=KCTC 23040(T)).


Assuntos
Oxalobacteraceae/classificação , Oxalobacteraceae/isolamento & purificação , Água do Mar/microbiologia , Aerobiose , Regiões Antárticas , Composição de Bases , Catalase/metabolismo , Análise por Conglomerados , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Ácidos Graxos/análise , Concentração de Íons de Hidrogênio , Locomoção , Dados de Sequência Molecular , Oxalobacteraceae/genética , Oxalobacteraceae/fisiologia , Oxirredutases/metabolismo , Fosfolipídeos/análise , Filogenia , Quinonas/análise , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Temperatura
9.
J Neurosci ; 30(43): 14458-69, 2010 Oct 27.
Artigo em Inglês | MEDLINE | ID: mdl-20980603

RESUMO

Negative transcriptional feedback loops are a core feature of eukaryotic circadian clocks and are based on rhythmic interactions between clock-specific repressors and transcription factors. In Drosophila, the repression of dCLOCK (dCLK)-CYCLE (CYC) transcriptional activity by dPERIOD (dPER) is critical for driving circadian gene expression. Although growing lines of evidence indicate that circadian repressors such as dPER function, at least partly, as molecular bridges that facilitate timely interactions between other regulatory factors and core clock transcription factors, how dPER interacts with dCLK-CYC to promote repression is not known. Here, we identified a small conserved region on dPER required for binding to dCLK, termed CBD (for dCLK binding domain). In the absence of the CBD, dPER is unable to stably associate with dCLK and inhibit the transcriptional activity of dCLK-CYC in a simplified cell culture system. CBD is situated in close proximity to a region that interacts with other regulatory factors such as the DOUBLETIME kinase, suggesting that complex architectural constraints need to be met to assemble repressor complexes. Surprisingly, when dPER missing the CBD (dPER(ΔCBD)) was evaluated in flies the clock mechanism was operational, albeit with longer periods. Intriguingly, the interaction between dPER(ΔCBD) and dCLK is TIM-dependent and modulated by light, revealing a novel and unanticipated in vivo role for TIM in circadian transcription. Finally, dPER(ΔCBD) does not provoke the daily hyperphosphorylation of dCLK, indicating that direct interactions between dPER and dCLK are necessary for the dCLK phosphorylation program but are not required for other aspects of dCLK regulation.


Assuntos
Proteínas CLOCK/fisiologia , Ritmo Circadiano/fisiologia , Proteínas de Drosophila/fisiologia , Proteínas Circadianas Period/fisiologia , Animais , Animais Geneticamente Modificados , Western Blotting , Proteínas CLOCK/genética , Células Cultivadas , Drosophila , Proteínas de Drosophila/genética , Imuno-Histoquímica , Luz , Atividade Motora/fisiologia , Proteínas Circadianas Period/genética , Fosforilação , Plasmídeos/genética , Ligação Proteica/genética , RNA/biossíntese , RNA/genética , Proteínas Repressoras/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Transcrição Genética/fisiologia
10.
Clin Vaccine Immunol ; 17(4): 503-12, 2010 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-20130128

RESUMO

The principal objectives of this study were to develop autologous antigen-presenting cells (APCs) and to characterize the antigen-specific T-cell responses to the M and N proteins of porcine reproductive and respiratory syndrome virus (PRRSV) by using those APCs in outbred pigs. The orf6 and orf7 genes fused with porcine granulocyte-macrophage colony-stimulating factor (GM-CSF) were cloned into the mammalian expression vector to generate two plasmid DNAs, namely, pcDNA3.1-GM-CSF-PRRSV-M and pcDNA3.1-GM-CSF-PRRSV-N. Three of six pigs in two groups were repeatedly immunized with either plasmid DNA construct, and four pigs were used as controls. The recombinant M and N proteins fused with the protein transduction domain (PTD) of the human immunodeficiency virus type 1 transactivator of transcription protein were employed to generate major histocompatibility complex-matched autologous APCs from each pig. The levels of T-cell proliferation and gamma interferon (IFN-gamma) synthesis were compared between pigs immunized with the two plasmid DNAs after stimulation of the peripheral blood mononuclear cells (PBMCs) of each pig with the autologous antigen-presenting dendritic cells and PBMCs. Higher levels of T-cell proliferation and IFN-gamma synthesis were identified in PBMCs isolated from the pigs immunized with pcDNA3.1-GM-CSF-PRRSV-M than in those isolated from the pigs immunized with pcDNA3.1-GM-CSF-PRRSV-N. By way of contrast, serum antibodies were detected only in pigs immunized with pcDNA3.1-GM-CSF-PRRSV-N. However, no T-cell response or antibody production was detected in the control pigs. These results suggest that the M protein of PRRSV is a more potent T cell-stimulating antigen than the N protein. Nevertheless, it should be emphasized that the N protein substantially induces both cellular and humoral immune responses. The newly developed protocol for generating self APCs may prove effective in further efforts to characterize additional PRRSV proteins involved in the induction of cell-mediated immunity.


Assuntos
Imunidade Celular , Vírus da Síndrome Respiratória e Reprodutiva Suína/imunologia , Proteínas Virais/imunologia , Animais , Proliferação de Células , Células Cultivadas , Células Dendríticas/imunologia , HIV-1/genética , Interferon gama/metabolismo , Plasmídeos/administração & dosagem , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/imunologia , Suínos , Linfócitos T/imunologia , Vacinação/métodos , Vacinas de DNA/administração & dosagem , Proteínas Virais/genética
11.
J Med Virol ; 82(4): 583-91, 2010 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-20166181

RESUMO

The hepatitis E virus (HEV) is an emerging zoonotic agent, for which pigs are the most important reservoir. Complete genome sequences of two swine HEV strains, designated swKOR-1 and swKOR-2, were determined via RT-PCR and RACE-PCR. The strains contained genomes composed of 7,222- and 7,221-bp excluding the poly(A) tails, respectively. The swKOR-1 and swKOR-2 strains were classified into subtype 3a of genotype 3 via phylogenetic analysis. These strains formed a distinctive cluster in the phylogenetic tree with human and swine HEVs isolated in the USA and human HEVs isolated in Japan. Anti-HEV antibodies were identified via ELISA in 8 of 99 (8.1%) cats, whereas, among 115 cattle and 213 dogs, no HEV-specific antibodies were detected. The conserved RNA-dependent RNA polymerase (RdRp) gene of HEV could be detected via RT-PCR in 8.7% of raw oysters collected from coastal regions in Korea. The HEV RNAs detected in oysters were identified as belonging to subtype 3a. The HEV RNAs in oysters most closely resembled that of the swKOR-2 strain. They also showed a close genetic relationship with the swKOR-1 strain and the swine and human HEVs isolated in the USA. This is the first report describing the detection in oysters of HEV that may have originated from genotype 3 swine HEV in Korea. Pigs and cats infected with HEV, as well as oysters contaminated with HEV, are potential risk factors for HEV transmission to humans.


Assuntos
Genoma Viral , Vírus da Hepatite E/classificação , Vírus da Hepatite E/genética , Hepatite E/transmissão , Hepatite E/veterinária , RNA Viral/genética , Zoonoses/transmissão , Animais , Doenças do Gato/imunologia , Doenças do Gato/transmissão , Doenças do Gato/virologia , Gatos , Bovinos , Doenças dos Bovinos/imunologia , Doenças dos Bovinos/virologia , Análise por Conglomerados , Doenças do Cão/imunologia , Doenças do Cão/virologia , Cães , Genótipo , Anticorpos Anti-Hepatite/sangue , Hepatite E/virologia , Vírus da Hepatite E/isolamento & purificação , Humanos , Coreia (Geográfico) , Epidemiologia Molecular , Dados de Sequência Molecular , Ostreidae/virologia , Filogenia , Fatores de Risco , Análise de Sequência de DNA , Suínos , Doenças dos Suínos/imunologia , Doenças dos Suínos/transmissão , Doenças dos Suínos/virologia , Zoonoses/virologia
12.
Neurol Res ; 32 Suppl 1: 116-9, 2010 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-20034459

RESUMO

OBJECTIVES: Cholecystokinin, a satiety hormone, acts on cholecystokinin A receptor on vagal afferent neurons that project to the nucleus tractus solitarius, resulting in inhibition of feeding. Cholecystokinin is known to be released by electroacupuncture stimulation at certain body sites which elicits profound psychophysiological responses. Our previous study has revealed the involvement of cholecystokinin and cholecystokinin A receptor in the electroacupuncture stimulation-induced modulation of feeding. The aim of the present study was to examine whether electroacupuncture stimulation at the acupuncture point ST36 (Joksamni) activates the nucleus tractus solitarius neurons and whether such effect is mediated by cholecystokinin A receptor. METHODS: Using an immunofluorescent analysis of Fos, a neuronal activation marker, we compared the Fos immunoreactivity of the nucleus tractus solitarius among three groups of Sprague-Dawley rats: (1) control (48 hour fasting + saline pre-treatment + no electroacupuncture stimulation); (2) SalEA (48 hour fasting + saline pre-treatment + ST36 electroacupuncture stimulation); (3) LorEA (48 hour fasting + pre-treatment of cholecystokinin A receptor antagonist, lorglumide + ST36 electroacupuncture stimulation). RESULTS: ST36 electroacupuncture stimulation significantly reduced 30 minute food intake (p<0.05, SalEA versus control) and increased Fos expression in the nucleus tractus solitarius (p<0.01, SalEA versus control). The effects of electroacupuncture on food intake and Fos were blocked by a lorglumide pre-treatment (p>0.05, LorEA versus control). DISCUSSION: Our finding suggests that ST36 electroacupuncture stimulation activates the nucleus tractus solitarius neurons via cholecystokinin A receptor signaling pathway, which may be the underlying central mechanism of electroacupuncture-induced satiety effect.


Assuntos
Ingestão de Alimentos/fisiologia , Eletroacupuntura/métodos , Jejum/fisiologia , Proteínas Proto-Oncogênicas c-fos/metabolismo , Receptor de Colecistocinina A/metabolismo , Núcleo Solitário/fisiologia , Animais , Ingestão de Alimentos/efeitos dos fármacos , Imunofluorescência , Antagonistas de Hormônios/farmacologia , Masculino , Neurônios/efeitos dos fármacos , Neurônios/fisiologia , Proglumida/análogos & derivados , Proglumida/farmacologia , Ratos , Ratos Sprague-Dawley , Transdução de Sinais/efeitos dos fármacos , Núcleo Solitário/efeitos dos fármacos
13.
Peptides ; 29(4): 564-70, 2008 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-18289731

RESUMO

A major satiety hormone, cholecystokinin (CCK) is well known to be released by electroacupuncture (EA) stimulation at certain body sites which elicits profound psychophysiological responses. Previous clinical and animal studies have shown that EA stimulation reduces food intake and body weight in both normal and obese subjects. The aim of the present study was to elucidate the satiety effect of EA stimulation and its mechanism related to CCK in rats. Here we show that EA stimulation at "Zusanli" (ST36) acupoint significantly reduced 30-min and 60-min food intake in 48-h fasted Sprague-Dawley rats, and such effect was reversed by a lorglumide (CCK-1 receptor antagonist, 10mg/kg, i.p.) pretreatment. The ST36 EA stimulation-induced satiety was not observed in CCK-1 receptor knockout, Otsuka Long-Evans Tokushima Fatty rats, but in their controls, Long-Evans Tokushima Otsuka rats. Subdiaphragmatic vagotomy also blocked the satiety effect of ST36 EA stimulation in Sprague-Dawley rats. These results suggest that ST36 EA stimulation elicits satiety in rats and this is mediated by the endogenous CCK signaling pathway.


Assuntos
Colecistocinina/metabolismo , Eletroacupuntura , Saciação/fisiologia , Animais , Jejum/fisiologia , Masculino , Proglumida/análogos & derivados , Proglumida/farmacologia , Ratos , Ratos Endogâmicos OLETF , Ratos Sprague-Dawley
14.
J Vet Med Sci ; 70(12): 1367-71, 2008 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-19122408

RESUMO

Hepatitis E virus (HEV) infection induces an acute hepatitis or a subclinical disease in humans. It is known that HEV is a zoonotic agent and pigs are major reservoirs of HEV. This study was conducted to determine the fecal shedding rates of HEV in various age groups of pigs and identify the genotypes of swine HEV prevailing in Korea. A total of 565 fecal samples were collected from suckling piglets, post-weaning pigs, growing pigs, and sows at 12 swine farms. RT-PCR was used to detect the presence of swine HEV in the feces. Every swine farm examined in this study had HEV-infected pigs. The fecal shedding rates of the swine HEV at individual farms were in the range of 2.1-35.4%. The overall fecal shedding rate of HEV in individual pigs was 17.5%. The HEV shedding rates of suckling piglets, post-weaning pigs, growing pigs and sows in their feces were 6.3, 16.3, 38.0 and 9.3%, respectively. When the genotypes of swine HEVs identified in this study were determined, they were all grouped into genotype 3. They were further subdivided into subtype 3a together with human and swine HEVs isolated in the U.S.A.


Assuntos
Fezes/virologia , Genótipo , Vírus da Hepatite E/genética , Doenças dos Suínos/virologia , Distribuição por Idade , Animais , Feminino , Coreia (Geográfico)/epidemiologia , Masculino , Filogenia , Suínos , Doenças dos Suínos/epidemiologia , Eliminação de Partículas Virais
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