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1.
Bioinformatics ; 2021 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-33538809

RESUMO

MOTIVATION: Narrative visualization for scientific data explorations can help users better understand the domain knowledge, because narrative visualizations often present a sequence of facts and observations linked together by a unifying theme or argument. Narrative visualization in immersive environments can provide users with an intuitive experience to interactively explore the scientific data, because immersive environments provide a brand new strategy for interactive scientific data visualization and exploration. However, it is challenging to develop narrative scientific visualization in immersive environments. In this paper, we propose an immersive narrative visualization tool to create and customize scientific data explorations for ordinary users with little knowledge about programming on scientific visualization, They are allowed to define POIs (point of interests) conveniently by the handler of an immersive device. RESULTS: Automatic exploration animations with narrative annotations can be generated by the gradual transitions between consecutive POI pairs. Besides, interactive slicing can be also controlled by device handler. Evaluations including user study and case study are designed and conducted to show the usability and effectiveness of the proposed tool. AVAILABILITY: Related information can be accessed at: https://dabigtou.github.io/richenliu/.

2.
IEEE Trans Cybern ; PP2020 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-33095735

RESUMO

Real-world multilabel data are high dimensional, and directly using them for label distribution learning (LDL) will incur extensive computational costs. We propose a multilabel distribution learning algorithm based on multioutput regression through manifold learning, referred to as MDLRML. By exploiting smooth, similar spaces' information provided by the samples' manifold learning and LDL, we link the two spaces' manifolds. This facilitates using the topological relationship of the manifolds in the feature space to guide the manifold construction of the label space. The smoothest regression function is used to fit the manifold data, and a locally constrained multioutput regression is designed to improve the data's local fitting. Based on the regression results, we enhance the logical labels into the label distributions, thereby mining and revealing the label's hidden information regarding importance or significance. Extensive experimental results using real-world multilabel datasets show that the proposed MDLRML algorithm significantly improves the multilabel distribution learning accuracy and efficiency over several existing state-of-the-art schemes.

3.
Comput Commun ; 162: 139-151, 2020 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-32873996

RESUMO

Frequency estimation of physical symptoms for peoples is the most direct way to analyze and predict infectious diseases. In Internet of medical Things (IoMT), it is efficient and convenient for users to report their physical symptoms to hospitals or disease prevention departments by various mobile devices. Unfortunately, it usually brings leakage risk of these symptoms since data receivers may be untrusted. As a strong metric for health privacy, local differential privacy (LDP) requires that users should perturb their symptoms to prevent the risk. However, the widely-used data structure called sketch for frequency estimation does not satisfy the specified requirement. In this paper, we firstly define the problem of frequency estimation of physical symptoms under LDP. Then, we propose four different protocols, i.e., CMS-LDP, FCS-LDP, CS-LDP and FAS-LDP to solve the above problem. Next, we demonstrate that the designed protocols satisfy LDP and unbiased estimation. We also present two approaches to implement the key component (i.e., universal hash functions) of protocols. Finally, we conduct experiments to evaluate four protocols on two real-world datasets, representing two different distributions of physical symptoms. The results show that CMS-LDP and CS-LDP have relatively optimal utility for frequency estimation of physical symptoms in IoMT.

4.
Technol Health Care ; 24 Suppl 2: S641-9, 2016 Apr 29.
Artigo em Inglês | MEDLINE | ID: mdl-27163327

RESUMO

It is important to detect abnormal brains accurately and early. The wavelet-energy (WE) was a successful feature descriptor that achieved excellent performance in various applications; hence, we proposed a WE based new approach for automated abnormal detection, and reported its preliminary results in this study. The kernel support vector machine (KSVM) was used as the classifier, and quantum-behaved particle swarm optimization (QPSO) was introduced to optimize the weights of the SVM. The results based on a 5 × 5-fold cross validation showed the performance of the proposed WE + QPSO-KSVM was superior to ``DWT + PCA + BP-NN'', ``DWT + PCA + RBF-NN'', ``DWT + PCA + PSO-KSVM'', ``WE + BPNN'', ``WE +$ KSVM'', and ``DWT $+$ PCA $+$ GA-KSVM'' w.r.t. sensitivity, specificity, and accuracy. The work provides a novel means to detect abnormal brains with excellent performance.


Assuntos
Encéfalo/fisiologia , Processamento de Imagem Assistida por Computador/métodos , Máquina de Vetores de Suporte , Análise de Ondaletas , Algoritmos , Humanos , Análise de Componente Principal , Sistemas de Informação em Radiologia
5.
Artigo em Inglês | MEDLINE | ID: mdl-26082713

RESUMO

PURPOSE: Early diagnosis or detection of Alzheimer's disease (AD) from the normal elder control (NC) is very important. However, the computer-aided diagnosis (CAD) was not widely used, and the classification performance did not reach the standard of practical use. We proposed a novel CAD system for MR brain images based on eigenbrains and machine learning with two goals: accurate detection of both AD subjects and AD-related brain regions. METHOD: First, we used maximum inter-class variance (ICV) to select key slices from 3D volumetric data. Second, we generated an eigenbrain set for each subject. Third, the most important eigenbrain (MIE) was obtained by Welch's t-test (WTT). Finally, kernel support-vector-machines with different kernels that were trained by particle swarm optimization, were used to make an accurate prediction of AD subjects. Coefficients of MIE with values higher than 0.98 quantile were highlighted to obtain the discriminant regions that distinguish AD from NC. RESULTS: The experiments showed that the proposed method can predict AD subjects with a competitive performance with existing methods, especially the accuracy of the polynomial kernel (92.36 ± 0.94) was better than the linear kernel of 91.47 ± 1.02 and the radial basis function (RBF) kernel of 86.71 ± 1.93. The proposed eigenbrain-based CAD system detected 30 AD-related brain regions (Anterior Cingulate, Caudate Nucleus, Cerebellum, Cingulate Gyrus, Claustrum, Inferior Frontal Gyrus, Inferior Parietal Lobule, Insula, Lateral Ventricle, Lentiform Nucleus, Lingual Gyrus, Medial Frontal Gyrus, Middle Frontal Gyrus, Middle Occipital Gyrus, Middle Temporal Gyrus, Paracentral Lobule, Parahippocampal Gyrus, Postcentral Gyrus, Posterial Cingulate, Precentral Gyrus, Precuneus, Subcallosal Gyrus, Sub-Gyral, Superior Frontal Gyrus, Superior Parietal Lobule, Superior Temporal Gyrus, Supramarginal Gyrus, Thalamus, Transverse Temporal Gyrus, and Uncus). The results were coherent with existing literatures. CONCLUSION: The eigenbrain method was effective in AD subject prediction and discriminant brain-region detection in MRI scanning.

7.
Comput Math Methods Med ; 2014: 546814, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24971155

RESUMO

The sampling patterns, cost functions, and reconstruction algorithms play important roles in optimizing compressed sensing magnetic resonance imaging (CS-MRI). Simple random sampling patterns did not take into account the energy distribution in k-space and resulted in suboptimal reconstruction of MR images. Therefore, a variety of variable density (VD) based samplings patterns had been developed. To further improve it, we propose a novel energy preserving sampling (ePRESS) method. Besides, we improve the cost function by introducing phase correction and region of support matrix, and we propose iterative thresholding algorithm (ITA) to solve the improved cost function. We evaluate the proposed ePRESS sampling method, improved cost function, and ITA reconstruction algorithm by 2D digital phantom and 2D in vivo MR brains of healthy volunteers. These assessments demonstrate that the proposed ePRESS method performs better than VD, POWER, and BKO; the improved cost function can achieve better reconstruction quality than conventional cost function; and the ITA is faster than SISTA and is competitive with FISTA in terms of computation time.


Assuntos
Encéfalo/patologia , Compressão de Dados/métodos , Imagem por Ressonância Magnética/métodos , Algoritmos , Voluntários Saudáveis , Humanos , Aumento da Imagem/métodos , Interpretação de Imagem Assistida por Computador/métodos , Processamento de Imagem Assistida por Computador , Modelos Teóricos , Imagens de Fantasmas , Probabilidade , Valores de Referência , Software
8.
Oncol Rep ; 32(1): 105-14, 2014 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-24840404

RESUMO

Adhesion of cancer cells to the extracellular matrix (ECM) causes a novel acquired chemotherapeutic drug­resistant phenotype, referred to as cell adhesion-mediated drug resistance (CAM-DR). Our previous studies suggested that the adhesion molecule MGr1-Ag/37LRP may promote multidrug resistance in gastric cancer cells. Therefore, we investigated MGr1-Ag/37LRP binding-induced adhesion, and its role in CAM-DR. Initial studies revealed that, after adhesion to the ECM, the multidrug-resistant gastric cancer cell lines SGC7901/VCR and SGC7901/ADR showed significantly higher mean adhesive cell numbers than non­resistant SGC7901 cells. We then investigated expression of MGr1-Ag/37LRP in gastric cancer cells adhering to laminin. Western blotting, RT-PCR and dual-luciferase reporter assays showed that laminin induced MGr1-Ag/37LRP expression and activity. In vitro and in vivo assays revealed that small interfering RNA against MGr1-Ag/37LRP significantly reduced CAM-DR in SGC7901/VCR cells. In vivo and in vitro analyses revealed that binding of MGr1-Ag/37LRP decreased intracellular drug accumulation by increasing P-glycoprotein and multidrug-associated protein expression, and inhibited drug-induced apoptosis by regulating Bcl-2 and Bax expression. These results indicate that MGr1-Ag/37LRP contributes to laminin-mediated CAM-DR in gastric cancer cells, and is a potentially effective target for reversing this phenomenon in gastric cancer.


Assuntos
Antígenos de Neoplasias/genética , Antígenos de Neoplasias/metabolismo , Resistencia a Medicamentos Antineoplásicos/efeitos dos fármacos , Matriz Extracelular/metabolismo , Laminina/metabolismo , Neoplasias Gástricas/patologia , Animais , Antineoplásicos Fitogênicos/uso terapêutico , Apoptose/efeitos dos fármacos , Adesão Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Feminino , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Humanos , Camundongos , Camundongos Nus , Neoplasias Experimentais , Neoplasias Gástricas/tratamento farmacológico , Neoplasias Gástricas/genética , Vincristina/uso terapêutico
9.
Cancer Sci ; 105(6): 651-9, 2014 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-24703465

RESUMO

It is well known that tumor microenvironment plays a vital role in drug resistance and cell adhesion-mediated drug resistance (CAM-DR), a form of de novo drug resistance. In our previous study, we reported that MGr1-Ag/37LRP ligation-induced adhesion participated in protecting gastric cancer cells from a number of apoptotic stimuli caused by chemotherapeutic drugs. Further study suggested that MGr1-Ag could prompt CAM-DR through interaction with laminin. However, the MGr1-Ag-initiated intracellular signal transduction pathway is still unknown. In this study, our experimental results showed that gastric cancer MDR cell lines mediated CAM-DR through upregulation of Bcl-2 by MGr1-Ag interaction with laminin. Further study found that, as a receptor of ECM components, MGr1-Ag/37LRP may activate the downstream signal pathway PI3K/AKT and MAPK/ERK through interaction with phosphorylated FAK. Moreover, the sensitivity to chemotherapeutic drugs could be significantly enhanced by inhibiting MGr1-Ag/37LRP expression through mAbs, siRNA, and antisense oligonucleotide. According to these results, we concluded that the FAK/PI3K and MAPK signal pathway plays an important role in MGr1-Ag-mediated CAM-DR in gastric cancer. MGr1-Ag/37LRP might be a potential effective reversal target to MDR in gastric cancer.


Assuntos
Antígenos de Neoplasias/metabolismo , Resistencia a Medicamentos Antineoplásicos , Proteínas Quinases Ativadas por Mitógeno/metabolismo , Fosfatidilinositol 3-Quinases/metabolismo , Receptores de Laminina/metabolismo , Proteínas Ribossômicas/metabolismo , Neoplasias Gástricas/tratamento farmacológico , Animais , Anticorpos Monoclonais/imunologia , Antígenos de Neoplasias/genética , Adesão Celular , Quinase 1 de Adesão Focal/metabolismo , Humanos , Laminina/metabolismo , Masculino , Camundongos , Camundongos Nus , Proteínas Proto-Oncogênicas c-bcl-2/biossíntese , Interferência de RNA , RNA Interferente Pequeno , Receptores de Laminina/genética , Proteínas Ribossômicas/genética , Transdução de Sinais , Neoplasias Gástricas/patologia , Células Tumorais Cultivadas , Microambiente Tumoral , Regulação para Cima , Vincristina/farmacologia
10.
ScientificWorldJournal ; 2013: 130134, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24163610

RESUMO

Automated abnormal brain detection is extremely of importance for clinical diagnosis. Over last decades numerous methods had been presented. In this paper, we proposed a novel hybrid system to classify a given MR brain image as either normal or abnormal. The proposed method first employed digital wavelet transform to extract features then used principal component analysis (PCA) to reduce the feature space. Afterwards, we constructed a kernel support vector machine (KSVM) with RBF kernel, using particle swarm optimization (PSO) to optimize the parameters C and σ . Fivefold cross-validation was utilized to avoid overfitting. In the experimental procedure, we created a 90 images dataset brain downloaded from Harvard Medical School website. The abnormal brain MR images consist of the following diseases: glioma, metastatic adenocarcinoma, metastatic bronchogenic carcinoma, meningioma, sarcoma, Alzheimer, Huntington, motor neuron disease, cerebral calcinosis, Pick's disease, Alzheimer plus visual agnosia, multiple sclerosis, AIDS dementia, Lyme encephalopathy, herpes encephalitis, Creutzfeld-Jakob disease, and cerebral toxoplasmosis. The 5-folded cross-validation classification results showed that our method achieved 97.78% classification accuracy, higher than 86.22% by BP-NN and 91.33% by RBF-NN. For the parameter selection, we compared PSO with those of random selection method. The results showed that the PSO is more effective to build optimal KSVM.


Assuntos
Encéfalo/metabolismo , Encéfalo/patologia , Processamento de Imagem Assistida por Computador/normas , Máquina de Vetores de Suporte , Algoritmos , Inteligência Artificial , Humanos , Análise de Componente Principal
11.
Med Oncol ; 30(2): 539, 2013 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-23519485

RESUMO

We have investigated the expression and role of the 58-kDa micro-spherule protein (MSP58) in hepatocellular carcinoma (HCC). Immunohistochemistry was performed in 252 samples from patients with HCC to detect the expression level of MSP58. Results indicated that the expression level of MSP58 in the cancer samples was significantly higher than that in adjacent normal tissues. The Wilcoxon-Mann-Whitney test showed significant difference in the expression of MSP58 in patients with serum AFP, tumor size, histological differentiation, and universal integrated circuit card (UICC) stage (P < 0.001, P = 0.004, P < 0.001, P < 0.001, respectively). A total of 252 HCC patients were followed up for five consecutive years, and Kaplan-Meier survival analysis demonstrated that the survival time of HCC patients with low expression of MSP58 was longer than those with high expression during the 5-year follow-up period (P < 0.001). Cox regression analysis indicated that high expression of MSP58 (++ or +++), serum AFP (≥25 µg/L), tumor size (≥3 cm), and UICC stage (III or IV) were the independent poor prognostic factors of HCC (P = 0.008, 0.0290, 0.001, 0.047, respectively). Furthermore, down-regulation of MSP58 was introduced to HCC cell lines (HepG2 and Huh7) by plasmid transfection. In vivo and in vitro studies indicated that MSP58si markedly reduced proliferation and promoted the apoptosis of HepG2 and Huh7 cells. In summary, our results demonstrated that MSP58 played an important role in the proliferation and apoptosis of HCC cells and the expression of MSP58 in HCC patients was closely related to the prognosis.


Assuntos
Biomarcadores Tumorais/biossíntese , Carcinoma Hepatocelular/metabolismo , Regulação Neoplásica da Expressão Gênica , Neoplasias Hepáticas/metabolismo , Proteínas Nucleares/biossíntese , Proteínas de Ligação a RNA/biossíntese , Adulto , Idoso , Animais , Biomarcadores Tumorais/genética , Carcinoma Hepatocelular/diagnóstico , Carcinoma Hepatocelular/mortalidade , Feminino , Seguimentos , Células Hep G2 , Humanos , Neoplasias Hepáticas/diagnóstico , Neoplasias Hepáticas/mortalidade , Masculino , Camundongos , Camundongos Nus , Pessoa de Meia-Idade , Proteínas Nucleares/genética , Proteínas de Ligação a RNA/genética , Taxa de Sobrevida/tendências
12.
Neurosci Lett ; 477(1): 28-32, 2010 Jun 14.
Artigo em Inglês | MEDLINE | ID: mdl-20416359

RESUMO

Exogenous adenosine produces potent synaptic inhibition in spinal substantia gelatinosa (SG), a region involved in nociceptive and thermoreceptive mechanisms. To examine the possibility that endogenous adenosine tonically modulates excitatory synaptic transmission in spinal SG, whole-cell, voltage-clamp recordings were made from SG neurons in adult rat spinal cord slices. In all SG neurons sensitive to exogenous adenosine, the adenosine uptake inhibitor, NBTI, mimics adenosine's inhibitory actions on dorsal root evoked EPSCs (eEPSCs) and miniature spontaneous EPSCs (mEPSCs). These inhibitory effects were antagonized by A1 adenosine receptor antagonist, DPCPX. DPCPX also potentates eEPSCs in those SG neurons in which adenosine or adenosine A1 receptor agonists (CHA, CCPA) suppressed eEPSCs. DPCPX often increases mEPSC frequency without altering mEPSC amplitude, suggesting presynaptic action on adenosine A1 receptors. Selective A2 (DMPX) and A2a (ZM 241385) adenosine receptor antagonists had no or minimal effects upon either eEPSCs or mEPSCs. The adenosine degrading enzyme, adenosine deaminase, mimicked the effects of DPCPX on the mEPSC frequency. We conclude that the excitatory synaptic transmission in the spinal SG is under an inhibitory tone of endogenous adenosine through the activation of A1 receptors. The present results suggested that the background activity of A1 receptors in the spinal SG might be contributed to setting the physiological "noceceptive thresholds".


Assuntos
Adenosina/fisiologia , Potenciais Pós-Sinápticos Excitadores , Substância Gelatinosa/fisiologia , Transmissão Sináptica , Agonistas do Receptor A1 de Adenosina , Antagonistas do Receptor A1 de Adenosina , Antagonistas do Receptor A2 de Adenosina , Adenosina Desaminase/metabolismo , Animais , Estimulação Elétrica , Potenciais Pós-Sinápticos em Miniatura , Técnicas de Patch-Clamp , Ratos , Ratos Sprague-Dawley , Tioinosina/análogos & derivados , Tioinosina/farmacologia , Xantinas/farmacologia
13.
Shock ; 34(2): 183-9, 2010 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-20160672

RESUMO

Multiple organ dysfunction syndrome (MODS) is one of the leading causes of death in the intensive care unit. Organ failure especially lung injury is highly associated with the mortality for MODS patients. Volatile anesthetic isoflurane (ISO) is one of the most widely used anesthetic agents, and ISO anesthesia has been reported to improve the survival rate and organ function in sepsis/MODS models. However, the application of anesthetic dose ISO in critically ill patients is limited. Compared with i.v. anesthetic pentobarbital treatment, we showed that twice inhalation of ISO at subanesthetic dose (0.7%, 0.5 minimum alveolar concentration) alleviated lung injury at 24 h after zymosan (ZY) injection and increased the 7-day survival rate from 10% to 45% in mice. We also showed that ISO exerted its protection by significantly improving the activities of superoxide dismutase and catalase in lung and serum when compared with those in pentobarbital-treated mice. The catalase inhibitor 3-amino-1, 2, 4-triazole partially abolished the protective effect of ISO in ZY-challenged mice. We conclude that subanesthetic dose ISO protects against ZY-induced generalized inflammation and its associated lung injury via enhancing the activities of antioxidant enzymes in mice, which may provide a new strategy for the treatment of critically ill patients.


Assuntos
Lesão Pulmonar Aguda/prevenção & controle , Inflamação/prevenção & controle , Isoflurano/administração & dosagem , Lesão Pulmonar Aguda/induzido quimicamente , Amitrol (Herbicida)/farmacologia , Animais , Catalase/antagonistas & inibidores , Catalase/metabolismo , Inflamação/induzido quimicamente , Inflamação/tratamento farmacológico , Isoflurano/uso terapêutico , Masculino , Camundongos , Insuficiência de Múltiplos Órgãos/tratamento farmacológico , Estresse Oxidativo , Superóxido Dismutase/metabolismo , Zimosan
14.
BMJ Case Rep ; 20102010 Jul 07.
Artigo em Inglês | MEDLINE | ID: mdl-22753161

RESUMO

BACKGROUND: Mucoceles resulting from cystadenomas of the ovary are uncommon. Although rare, rupture of the mucoceles can occur without causing any abdominal issue. This report concerns an unusual and interesting case of the right ovary associated with liver, spleen and kidney cysts. CASE PRESENTATION: A case of ruptured mucocele resulting from cystadenoma of the ovary occurred in a 63-year-old woman. The patient underwent x-ray, ultrasound and a CT scan. Localised pseudomyxoma peritonei associated with adenocarcinoma of the right ovary was diagnosed. The patient underwent resection of the ovary, uterus, uterine tube and appendix with excision of the mesenteric lymph nodes and removal of about 5000 ml of yellowish mucoid jelly. She was free of symptoms at 1-year follow-up. CONCLUSIONS: Pseudomyxoma peritonei may occur in patients with ovarian cystadenocarcinoma. In such patients, the ovary should be investigated and transvaginal ultrasound can be carefully performed in cases of ruptured mucoceles and localised pseudomyxoma peritonei. Surgical intervention is the current choice of management.


Assuntos
Cistadenocarcinoma/patologia , Cistadenocarcinoma/cirurgia , Mucocele/secundário , Mucocele/cirurgia , Neoplasias Ovarianas/patologia , Neoplasias Ovarianas/cirurgia , Neoplasias Peritoneais/secundário , Neoplasias Peritoneais/cirurgia , Pseudomixoma Peritoneal/patologia , Pseudomixoma Peritoneal/cirurgia , Biomarcadores Tumorais/análise , Diagnóstico Diferencial , Diagnóstico por Imagem , Feminino , Humanos , Excisão de Linfonodo , Pessoa de Meia-Idade
15.
Shock ; 33(6): 646-54, 2010 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-19789462

RESUMO

Sepsis/multiple organ dysfunction syndrome (MODS) is a major cause of high mortality in the intensive care unit. We have recently reported that 100% oxygen treatment is beneficial to mice with zymosan-induced sterile inflammation by increasing antioxidant enzymatic activities. Yet, the use of hyperoxia is hindered by concerns that it could exacerbate organ injury by increasing free radical formation. It is believed that systemic inflammation and overproduction of reactive oxygen species (ROS) contribute to the mechanism underlying sepsis/MODS. A ROS scavenger has been proven to protect against sepsis/MODS in some animal models. Therefore, we hypothesized that ROS scavenger pretreatment might enhance the protective action of 100% oxygen treatment against zymosan-induced sterile inflammation in mice. In the present study, we showed that 100% oxygen treatment prevented the abnormal changes in serum biochemical parameters, tissue oxygenation, and organ histopathology, and improved the 14-day survival rate in zymosan-stimulated mice, indicating that 100% oxygen treatment had a protective action on sterile inflammation. We found that pretreatment with a ROS scavenger (N-acetylcysteine, vitamin C, or dimethylthiourea) abolished this protective action of 100% oxygen treatment. We also showed that 100% oxygen treatment decreased the levels of serum proinflammatory cytokines (TNF-alpha, IL-6, and high-mobility group box 1), increased the level of serum anti-inflammatory cytokine (IL-10), and upregulated the activities of serum and tissue antioxidant enzymes (superoxide dismutase, catalase, and glutathione peroxidase) in zymosan-stimulated mice, which were reversed by the pretreatment with a ROS scavenger (N-acetylcysteine, vitamin C, or dimethylthiourea). We thus conclude that ROS scavenger pretreatment partly abolishes the protective effects of 100% oxygen treatment on sterile inflammation in mice by regulating inflammatory cytokines as well as antioxidant enzymes.


Assuntos
Acetilcisteína/uso terapêutico , Depuradores de Radicais Livres/uso terapêutico , Oxigênio/uso terapêutico , Acetilcisteína/efeitos adversos , Animais , Antioxidantes/metabolismo , Ácido Ascórbico/efeitos adversos , Citocinas/sangue , Depuradores de Radicais Livres/efeitos adversos , Coração/efeitos dos fármacos , Inflamação/induzido quimicamente , Rim/efeitos dos fármacos , Rim/patologia , Fígado/efeitos dos fármacos , Fígado/patologia , Pulmão/efeitos dos fármacos , Pulmão/patologia , Masculino , Camundongos , Insuficiência de Múltiplos Órgãos/induzido quimicamente , Insuficiência de Múltiplos Órgãos/tratamento farmacológico , Insuficiência de Múltiplos Órgãos/patologia , Miocárdio/patologia , Espécies Reativas de Oxigênio/farmacologia , Sepse/prevenção & controle , Tioureia/efeitos adversos , Tioureia/análogos & derivados , Zimosan
16.
J Chem Neuroanat ; 39(3): 166-74, 2010 May.
Artigo em Inglês | MEDLINE | ID: mdl-19931378

RESUMO

In the central nervous system (CNS), endomorphin 1 (EM1)- and endomorphin 2 (EM2)-containing neuronal cell bodies have been found in the nucleus tractus sollitarii (NTS) and the hypothalamus, and EMergic fibers and terminals are distributed widely in many regions of the CNS, including the periaqueductal gray (PAG). The aim of the present study was to examine whether EM-expressing neurons in the NTS of the rat send their axons to the PAG, and determine whether the EMergic pathway from the NTS to the PAG is topographic by using. Immunofluorescent staining for EM1 or EM2 combined with retrograde and anterograde tract-tracing methods. The results showed that after injecting tetramethyl rhodamine dextran-amine (TMR) into the ventrolateral or lateral column of the PAG, some EM1- or EM2-immunoreactive (IR) neurons in the NTS were retrogradely labeled with TMR, and the majority of the EM-IR/TMR double-labeled neurons were mainly distributed in the medial and commissural subnuclei of the NTS. Following injection of biotinylated dextran amine (BDA) into the medial or commissural subnucleus of the NTS, EM1-IR/BDA and EM2-IR/BDA double-labeled fibers and terminals were mainly distributed in the ventrolateral or lateral column of the PAG, respectively. The results indicate that EMergic pathway from the NTS to PAG is topographically organized, and suggest that EMs released from NTS to PAG projecting terminals may bind to mu-opioid receptor on the PAG neurons, and thereby contribute to various functions.


Assuntos
Vias Neurais/anatomia & histologia , Substância Cinzenta Periaquedutal/anatomia & histologia , Núcleo Solitário/anatomia & histologia , Animais , Imunofluorescência , Masculino , Vias Neurais/metabolismo , Oligopeptídeos/metabolismo , Substância Cinzenta Periaquedutal/metabolismo , Ratos , Ratos Sprague-Dawley , Núcleo Solitário/metabolismo
17.
Hybridoma (Larchmt) ; 28(5): 373-6, 2009 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-19857120

RESUMO

Cis-aconitic acid decarboxylase (CAD) has been assumed to be a key enzyme in the production of itaconic acid. Here we aimed to efficiently generate the monoclonal antibody against the CAD protein. We synthesized the peptide "pdnaelvlltlgqawqg" based on the published CAD cDNA sequences. The peptide was chemically linked with the carrier protein keyhole limpet hemocyanin and then injected into Balb/c mice. Hybridomas were screened by indirect enzyme-linked immunosorbent assay (ELISA) using either purified 6 x His-CAD fusion protein or the peptide. One MAb named K2 (IgG1), effective in detecting the native CAD protein, was characterized by ELISA and Western immunoblotting. By using the MAb, we found that the CAD protein was more highly expressed in poorly differentiated gastric cancer tissues than in well-differentiated and moderately differentiated tissues. Taken together, the MAb K2 would be helpful for understanding the functions of CAD in gastric carcinogenesis.


Assuntos
Anticorpos Monoclonais/imunologia , Peptídeos/imunologia , Sequência de Aminoácidos , Animais , Western Blotting , Ensaio de Imunoadsorção Enzimática , Imuno-Histoquímica , Camundongos , Camundongos Endogâmicos BALB C , Dados de Sequência Molecular , Peptídeos/química , Proteínas Recombinantes/imunologia , Neoplasias Gástricas/enzimologia
18.
Anesth Analg ; 109(5): 1651-5, 2009 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-19713253

RESUMO

BACKGROUND: The treatment of postherpetic neuralgia (PHN) continues to be a challenge in clinical pain management. In this randomized, controlled study, we assessed the effectiveness of repetitive paravertebral injections with local anesthetics and steroids for the prevention of PHN in patients with acute herpes zoster. METHODS: One hundred thirty-two patients with acute herpes zoster diagnosed 1-7 days after the onset of the rash were randomly assigned to receive either standard therapy (oral antivirals and analgesics) or standard therapy with additional repetitive paravertebral injections of a mixture of 10 mL 0.25% bupivacaine and 40 mg methylprednisolone acetate every 48 h for a week. Efficacy was evaluated at 1, 3, 6, and 12 mo after the end of the treatments. The primary end point was the proportion of patients with zoster-associated pain and/or allodynia 1 mo after inclusion. Statistical analysis was performed based on the intent-to-treat population. RESULTS: One hundred thirteen patients completed the 1-yr follow-up. At 1 mo posttherapy, 13% of patients in the paravertebral group reported zoster-related pain, compared with 45% in the standard group (P < 0.001). At 3, 6, and 12 mo posttherapy, the incidence of PHN was still significantly lower in the paravertebral group than in the standard group. The quality of life improved in both groups at each follow-up time point with no significant difference between groups. CONCLUSION: Repetitive paravertebral anesthetic block in combination with steroids plus standard treatment with acyclovir and analgesics significantly reduced the incidence of PHN than the standard treatment alone.


Assuntos
Anestésicos Locais/administração & dosagem , Bupivacaína/administração & dosagem , Herpes Zoster/prevenção & controle , Metilprednisolona/análogos & derivados , Bloqueio Nervoso , Neuralgia Pós-Herpética/prevenção & controle , Esteroides/administração & dosagem , Doença Aguda , Aciclovir/administração & dosagem , Administração Oral , Idoso , Analgésicos/administração & dosagem , Antivirais/administração & dosagem , Esquema de Medicação , Combinação de Medicamentos , Quimioterapia Combinada , Feminino , Herpes Zoster/complicações , Humanos , Injeções , Masculino , Metilprednisolona/administração & dosagem , Acetato de Metilprednilosona , Pessoa de Meia-Idade , Neuralgia Pós-Herpética/virologia , Medição da Dor , Estudos Prospectivos , Qualidade de Vida , Fatores de Tempo , Resultado do Tratamento
19.
Chin Med J (Engl) ; 122(8): 950-4, 2009 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-19493421

RESUMO

BACKGROUND: Vascular hyporeactivity, which occurs in the terminal stage of hemorrhagic shock, is believed to be critical for treating hemorrhagic shock. The present study was designed to examine whether the CB1 cannabinoid receptor (CB1R) was involved in the development of vascular hyporeactivity in rats suffering from hemorrhagic shock. METHODS: Sixteen animals were randomly divided into two groups (n = 8 in each group): sham-operated (Sham) and hemorrhagic shock (HS) groups. Hemorrhagic shock was induced by bleeding. The mean arterial pressure (MAP) was reduced to and stabilized at (25 +/- 5) mmHg for 2 hours. The vascular reactivity was determined by the response of MAP to norepinephrine (NE). In later experiments another twelve animals were used in which the changes of CB1R mRNA and protein in aorta and superior mesenteric artery (SMA) were analyzed by RT-PCR and Western blotting. In addition, we investigated the effects of a CB1R antagonist on the vascular hyporeactivity and survival rates in rats with hemorrhagic shock. Survival rates were analyzed by the Fisher's exact probability test. The MAP response was analyzed by one-way analysis of variance (ANOVA). RESULTS: Vascular hyporeactivity developed in all animals suffering from hemorrhagic shock. The expression of CB1R mRNA and protein in aorta and 2 - 3 branches of the SMA were significantly increased in the HS group after the development of vascular hyporeactivity when compared to those in Sham group. When SR141716A or AM251 was administered, the MAP response to NE was (41.75 +/- 4.08) mmHg or (44.78 +/- 1.80) mmHg respectively, which was higher than that in saline groups with (4.31 +/- 0.36) mmHg (P < 0.01). We also showed an increased 4-hour survival rate in the SR141716A or AM251-treated group with 20% or 30%, but with a statistically significant difference present between the AM251-treated and saline groups (P < 0.05). CONCLUSIONS: CB1R is involved in vascular hyporeactivity resulting from hemorrhagic shock in rats, and CB1R antagonist may be useful in treating patients with traumatic, hemorrhagic shock who need field-rescue or initial treatment.


Assuntos
Receptor CB1 de Canabinoide/fisiologia , Choque Hemorrágico/metabolismo , Animais , Western Blotting , Regulação da Expressão Gênica/efeitos dos fármacos , Hipotensão/metabolismo , Masculino , Piperidinas/farmacologia , Pirazóis/farmacologia , Distribuição Aleatória , Ratos , Ratos Sprague-Dawley , Receptor CB1 de Canabinoide/antagonistas & inibidores , Receptor CB1 de Canabinoide/genética , Receptor CB1 de Canabinoide/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Rimonabanto , Choque Hemorrágico/mortalidade , Taxa de Sobrevida
20.
Chin Med J (Engl) ; 122(4): 449-54, 2009 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-19302753

RESUMO

BACKGROUND: Sepsis is a leading cause of death in the intensive care units. The late inflammatory cytokine, high-mobility group box 1 (HMGB1), plays a critical role in sepsis. In the present study, we investigated the association between the serum HMGB1 levels and the severity of organ injury in the lipopolysaccharide-induced sepsis in rats. METHODS: To produce an animal model of sepsis with different degree of organ injury, animals were treated with three different doses of lipopolysaccharide (4, 8 and 16 mg/kg), and the animals in control group were treated with the same volume of the vehicle (saline). The levels of serum HMGB1 were measured at 0, 2, 4, 8, 16, 24, 32 and 48 hours after lipopolysaccharide (LPS) or vehicle injection, meanwhile the biochemical and histopathological indicators for the severity of organ injury were assessed. RESULTS: The level of HMGB1 had a positive, high correlation with the abnormal changes of serum cardiac troponin I, alanine aminotransferase, aspartate aminotransferase, creatinine and blood urea nitrogen, as well as the pathologic scores of heart, lung, liver and kidney. CONCLUSIONS: The level of serum HMGB1 is highly correlated with the severity of sepsis in rats, suggesting that HMGB1 could serve as a valuable adjunct in the diagnosis and management of sepsis.


Assuntos
Proteína HMGB1/sangue , Sepse/sangue , Animais , Lipopolissacarídeos/uso terapêutico , Masculino , Distribuição Aleatória , Ratos , Ratos Sprague-Dawley , Sepse/tratamento farmacológico , Sepse/patologia
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