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Medicine (Baltimore) ; 98(7): e14368, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30762736


RATIONALE: Hepatocellular carcinoma (HCC) is the fifth most common cancer and third leading cause of cancer-related deaths worldwide. Nasopharyngeal metastasis of hepatocellular carcinoma is very rare. This is the first report of posttransplantation nasopharyngeal metastasis. PATIENT CONCERNS: A 45-year-old man with a history of hepatitis B related hepatocellular carcinoma (HCC) in the right segment of the liver received an orthotopic liver transplant. Two year after the transplantation, he suffered from severe headache, and head contrast enhanced CT scans did not show clues for brain or skull metastasis. Then he developed hoarseness and dysphagia. DIAGNOSIS: The nasopharyngeal cancer was confirmed to be metastatic tumor from liver histologically according to biopsy. INTERVENTIONS: This patient underwent radiotherapy (RT) of the metastatic nasopharyngeal tumor, and there was significant symptomatic relief. OUTCOMES: The patient died 3 months after nasopharyngeal metastasis was diagnosed. LESSONS: Recurrence of hepatocellular carcinoma with metastasis of nasopharyngeal carcinoma after liver transplantation is rare, but the prognosis is very poor. Close follow-up of patients should be paid attention to prevent the occurrence of such diseases.

Carcinoma Hepatocelular/patologia , Neoplasias Hepáticas/patologia , Neoplasias Nasofaríngeas/secundário , Carcinoma Hepatocelular/complicações , Carcinoma Hepatocelular/cirurgia , Hepatite B/complicações , Humanos , Neoplasias Hepáticas/complicações , Neoplasias Hepáticas/cirurgia , Transplante de Fígado , Masculino , Pessoa de Meia-Idade , Neoplasias Nasofaríngeas/radioterapia
Mediators Inflamm ; 2015: 762709, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25892855


AIMS: This study aimed to assess the differential expression of specific B cell subtypes in patients with chronic viral hepatitis. METHODS: The frequencies of differential expression of specific B cell subtypes in patients with chronic viral hepatitis and healthy controls were assessed by flow cytometry using monoclonal antibodies specific for CD38, CD27, CD86, CD95, TLR-9, and IgD. The effect of adefovir treatment on B cell subsets in HBV patients was determined. The values of clinical parameters in the patients were also measured. RESULTS: The frequency of CD86+ B cells was not significantly different in chronic HBV patients but was higher in HCV patients compared with that in healthy controls. CD95 and IgD levels were lower in HBV and HCV patients than in healthy controls. A significant negative correlation occurred between the proportion of CD95+ B cells and HBV DNA viral load. The frequency of TLR-9 on the B cells in HBV and HCV patients was higher compared with that of healthy controls. After treatment with adefovir, the frequency of CD95 and IgD expressed on B cells was increased in HBV patients. CONCLUSIONS: Activated B cells and exhausted B cells homeostasis were commonly disturbed in HBV and HCV patients.

Antígeno B7-2/sangue , Hepatite B Crônica/sangue , Hepatite B Crônica/tratamento farmacológico , Hepatite C Crônica/sangue , Hepatite C Crônica/tratamento farmacológico , Receptor Toll-Like 9/sangue , Receptor fas/sangue , Adenina/análogos & derivados , Adenina/uso terapêutico , Adulto , Idoso , Anticorpos Monoclonais/química , Antivirais/uso terapêutico , Linfócitos B/virologia , DNA Viral/sangue , Feminino , Citometria de Fluxo , Genótipo , Homeostase , Humanos , Imunoglobulina D/sangue , Leucócitos Mononucleares/citologia , Masculino , Pessoa de Meia-Idade , Oligodesoxirribonucleotídeos/química , Organofosfonatos/uso terapêutico , Fenótipo , Prevalência , Carga Viral , Adulto Jovem
World J Gastroenterol ; 20(18): 5474-82, 2014 May 14.
Artigo em Inglês | MEDLINE | ID: mdl-24833877


AIM: To investigate the feasibility of lectin microarray for differentiating gastric cancer from gastric ulcer. METHODS: Twenty cases of human gastric cancer tissue and 20 cases of human gastric ulcer tissue were collected and processed. Protein was extracted from the frozen tissues and stored. The lectins were dissolved in buffer, and the sugar-binding specificities of lectins and the layout of the lectin microarray were summarized. The median of the effective data points for each lectin was globally normalized to the sum of medians of all effective data points for each lectin in one block. Formalin-fixed paraffin-embedded gastric cancer tissues and their corresponding gastric ulcer tissues were subjected to Ag retrieval. Biotinylated lectin was used as the primary antibody and HRP-streptavidin as the secondary antibody. The glycopatterns of glycoprotein in gastric cancer and gastric ulcer specimens were determined by lectin microarray, and then validated by lectin histochemistry. Data are presented as mean ± SD for the indicated number of independent experiments. RESULTS: The glycosylation level of gastric cancer was significantly higher than that in ulcer. In gastric cancer, most of the lectin binders showed positive signals and the intensity of the signals was stronger, whereas the opposite was the case for ulcers. Significant differences in the pathological score of the two lectins were apparent between ulcer and gastric cancer tissues using the same lectin. For MPL and VVA, all types of gastric cancer detected showed stronger staining and a higher positive rate in comparison with ulcer, especially in the case of signet ring cell carcinoma and intra-mucosal carcinoma. GalNAc bound to MPL showed a significant increase. A statistically significant association between MPL and gastric cancer was observed. As with MPL, there were significant differences in VVA staining between gastric cancer and ulcer. CONCLUSION: Lectin microarray can differentiate the different glycopatterns in gastric cancer and gastric ulcer, and the lectins MPL and VVA can be used as biomarkers.

Biomarcadores Tumorais/análise , Glicoproteínas/análise , Lectinas , Neoplasias Gástricas/química , Úlcera Gástrica/metabolismo , Análise Serial de Tecidos , Adulto , Idoso , Biotinilação , Diagnóstico Diferencial , Estudos de Viabilidade , Feminino , Glicosilação , Humanos , Técnicas Imunoenzimáticas , Masculino , Pessoa de Meia-Idade , Lectinas de Plantas , Valor Preditivo dos Testes , Neoplasias Gástricas/patologia , Úlcera Gástrica/diagnóstico
Ann Hepatol ; 12(4): 539-47, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23813131


BACKGROUND: During the early phases of a hepatitis C virus (HCV) infection, NK cell activation appears to be critical to the induction of adaptive immune responses that have the potential of clearing the infection. This study aimed to investigate the phenotype and function of NK cells in chronic HCV (CHC) patients, particularly patients who cleared HCV infections spontaneously (SR-HCV). MATERIAL AND METHODS: Peripheral blood NK cells were compared between 36 CHC patients, 12 SR-HCV patients, and 14 healthy controls (HC). The phenotype and function of NK cells were characterized by flow cytometry. In addition, the potential associations between the frequency of NK cell subsets and ALT, AST and HCV viral loads were also analyzed. RESULTS: Our data revealed that the population of CD3-CD56+ NK cells was significantly decreased in CHC and SR-HCV patients compared to levels in HC (P = 0.031, P = 0.014). Interestingly, we found that the levels of the CD158b inhibitory receptor were higher in CHC patients compared to levels observed in HCand SR-HCV subjects (P = 0.018, P = 0.036). In addition, the percentages of the activation receptors NKp30 and NKp46 were significantly decreased in CHC and SR-HCV patients compared to their expression levels in HC (P < 0.05). Moreover, the frequencies of inducible CD107a (but not IFN-γ-secreting) NK cellsfrom both CHC and SR-HCV patients were significantly lower than frequencies observed in controls (P = 0.018, P = 0.027). CONCLUSION: Our data indicated that the higher frequency of inhibitory NK cells combined with fewer activated NK cells may be associated with HCV-related chronic inflammation involved in CHC pathogenesis.

Imunidade Adaptativa , Hepatite C Crônica/imunologia , Células Matadoras Naturais/imunologia , Ativação Linfocitária , Receptores KIR2DL3/sangue , Adulto , Idoso , Alanina Transaminase/sangue , Biomarcadores/sangue , Estudos de Casos e Controles , Feminino , Citometria de Fluxo , Hepatite C Crônica/sangue , Hepatite C Crônica/diagnóstico , Humanos , Imunofenotipagem/métodos , Células Matadoras Naturais/virologia , Contagem de Linfócitos , Glicoproteínas de Membrana Associadas ao Lisossomo/sangue , Masculino , Pessoa de Meia-Idade , Receptor 1 Desencadeador da Citotoxicidade Natural/sangue , Receptor 3 Desencadeador da Citotoxicidade Natural/sangue , Fenótipo , Adulto Jovem
Clin Exp Pharmacol Physiol ; 40(3): 190-6, 2013 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-23278368


The aim of the present study was to investigate the natural killer (NK) cell phenotype and function in chronic hepatitis B virus (HBV) patients and to study the effects of entecavir therapy (10 mg/day, p.o.) on these responses. Peripheral blood NK cells were collected from 18 chronic HBV patients and 14 healthy controls. The effect of entecavir therapy on the phenotype and function of NK cells in chronic HBV patients was characterized by flow cytometry analysis. Concentrations of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), HBV viral loads in both groups and potential associations between the frequency of peripheral NK cell subsets and clinical measures were determined. There was a significant reduction in the number of CD3(-)CD56(+) NK cells in chronic HBV patients compared with healthy controls. Furthermore, there were significant increases in the percentage of CD3(-)CD56(+)NKG2D(+) and CD3(-)CD56(+)NKP30(+) NK activating receptors in chronic HBV patients compared with healthy individuals, who exhibited downregulated expression following entecavir treatment. Spearman's correlation analysis revealed that there was a significant positive correlation between the percentage of NKG2D(+) and NKP30(+) NK cells and serum ALT levels. Characterization of NK cell degranulation indicated that the frequency of CD107a(+) NK cells in HBV patients (in response to K562 stimulation) was significantly greater than in healthy controls but decreased following entecavir treatment. Entecavir treatment of hepatitis B e antigen-positive chronic HBV-infected patients not only led to a reduction in HBV DNA loads and normalization of ALT and AST levels, but also resulted in the recovery of NK cell-mediated immunity.

Antivirais/uso terapêutico , Guanina/análogos & derivados , Hepatite B Crônica/tratamento farmacológico , Imunidade Celular/efeitos dos fármacos , Células Matadoras Naturais/efeitos dos fármacos , Receptores de Células Matadoras Naturais/biossíntese , Adulto , Antivirais/administração & dosagem , Complexo CD3/biossíntese , Antígeno CD56/biossíntese , Regulação para Baixo , Feminino , Citometria de Fluxo , Guanina/administração & dosagem , Guanina/uso terapêutico , Anticorpos Anti-Hepatite B/sangue , Anticorpos Anti-Hepatite B/imunologia , Antígenos de Superfície da Hepatite B/sangue , Antígenos de Superfície da Hepatite B/imunologia , Vírus da Hepatite B/efeitos dos fármacos , Hepatite B Crônica/imunologia , Hepatite B Crônica/virologia , Humanos , Células Matadoras Naturais/citologia , Células Matadoras Naturais/imunologia , Testes de Função Hepática , Masculino , Pessoa de Meia-Idade , Subfamília K de Receptores Semelhantes a Lectina de Células NK/biossíntese , Receptor 3 Desencadeador da Citotoxicidade Natural/biossíntese , Resultado do Tratamento , Carga Viral , Adulto Jovem