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1.
Carbohydr Polym ; 300: 120269, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36372492

RESUMO

An intelligent bionic trilayer fibrous membrane is developed via electrospinning for the moisture-adjusting of a storage space. The trilayer is composed of a hydrophobic inner layer of cellulose acetate (CA), a super hygroscopic intermediate layer of gelatin (GA) and a hydrophilic outer layer of ethylene-vinyl alcohol copolymer (EVOH). The hierarchical pore networks of EVOH/GA/CA (∼2.45/∼4.5/∼36.0 µm) and the asymmetry wettability endow the membrane with outstanding directional water transport capacity. Specifically, the membrane has an excellent accumulative one-way transport index (1293 %), a remarkable overall moisture management capacity (0.91) and a reasonably high water evaporation rate (0.59 g h-1). The target membrane can regulate the relative humidity (RH) from 75 % to 50 % without extra energy consumption, which is capable of extending the shelf-life of jerk beef by ∼100 % under surrounding temperature of 25 °C and humidity of 75 % RH.


Assuntos
Biônica , Gelatina , Animais , Bovinos , Água/química
2.
Sci Total Environ ; 856(Pt 1): 158977, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36155040

RESUMO

Nitrogen and phosphorus contamination in wastewater is a serious environmental concern and poses a global threat to sustainable development. In this paper, a comprehensive review of the studies on simultaneous nitrogen and phosphorus removal (SNPR) during 1986-2022 (538 publications) was conducted using bibliometrics, which showed that simultaneous nitrification, denitrification, and phosphorus removal (SNDPR) is the most promising process. To better understand SNDPR, the dissolved oxygen, carbon to nitrogen ratio, carbon source type, sludge retention time, Cu2+ and Fe3+, pH, salinity, electron acceptor type of denitrifying phosphorus-accumulating organisms (DPAOs), temperature, and other influencing factors were analyzed. Currently, SNDPR has been successfully implemented in activated sludge systems, aerobic granular sludge systems, biofilm systems, and constructed wetlands; sequential batch mode of operation is a common means to achieve this process. SNDPR exhibits a significant potential for phosphorus recovery. Future research needs to focus on: (1) balancing the competitiveness between denitrifying glycogen-accumulating organisms (DGAOs) and DPAOs, and countermeasures to deal with the effects of adverse conditions on SNDPR performance; (2) achieving SNDPR in continuous flow operation; and (3) maximizing the recovery of P during SNDPR to achieve resource sustainability. Overall, this study provides systematic and valuable information for deeper insights into SNDPR, which can help in further research.


Assuntos
Nitrificação , Fósforo , Esgotos , Desnitrificação , Eliminação de Resíduos Líquidos , Reatores Biológicos , Nitrogênio , Carbono
3.
Neural Regen Res ; 18(1): 141-149, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-35799534

RESUMO

Neuroinflammation and the NACHT, LRR, and PYD domains-containing protein 3 inflammasome play crucial roles in secondary tissue damage following an initial insult in patients with traumatic brain injury (TBI). Maraviroc, a C-C chemokine receptor type 5 antagonist, has been viewed as a new therapeutic strategy for many neuroinflammatory diseases. We studied the effect of maraviroc on TBI-induced neuroinflammation. A moderate-TBI mouse model was subjected to a controlled cortical impact device. Maraviroc or vehicle was injected intraperitoneally 1 hour after TBI and then once per day for 3 consecutive days. Western blot, immunohistochemistry, and TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling) analyses were performed to evaluate the molecular mechanisms of maraviroc at 3 days post-TBI. Our results suggest that maraviroc administration reduced NACHT, LRR, and PYD domains-containing protein 3 inflammasome activation, modulated microglial polarization from M1 to M2, decreased neutrophil and macrophage infiltration, and inhibited the release of inflammatory factors after TBI. Moreover, maraviroc treatment decreased the activation of neurotoxic reactive astrocytes, which, in turn, exacerbated neuronal cell death. Additionally, we confirmed the neuroprotective effect of maraviroc using the modified neurological severity score, rotarod test, Morris water maze test, and lesion volume measurements. In summary, our findings indicate that maraviroc might be a desirable pharmacotherapeutic strategy for TBI, and C-C chemokine receptor type 5 might be a promising pharmacotherapeutic target to improve recovery after TBI.

4.
Chembiochem ; 2022 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-36346708

RESUMO

Inspired by biological systems, peptides can self-assemble into various hierarchical nanostructures through non-covalent interactions and form functional materials exhibiting excellent chemical and physical propertie, which have broad applications in bio-/nano-technologic fields. The self-assembly mechanism, self-assembly morphology of peptide supramolecula architecture and their various applications have been widely explored with the merit of biocompatible, easy preparation, and controllable functionality. Herein, we introduce the latest research progress of self-assembling peptide-based nanomaterials and review their applications in biomedicine and optoelectronics, including tissue engineering, anticancer therapy, biomimetic catalysis, energy harvesting, etc. We believe that this review will inspire the rational design and development of novel peptide-based functional bio-inspired materials in the future.

5.
Small ; : e2205071, 2022 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-36366943

RESUMO

High-capacity electrochemical energy storage systems are more urgently needed than ever before with the rapid development of electric vehicles and the smart grid. The most efficient way to increase capacity is to develop electrode materials with low molecular weights. The low-cost metal halides are theoretically ideal cathode materials due to their advantages of high capacity and redox potential. However, their cubic structure and large energy barrier for deionization impede their rechargeability. Here, the reversibility of potassium halides, lithium halides, sodium halides, and zinc halides is achieved through decreasing their dimensionality by the strong π-cation interactions between metal cations and reduced graphene oxide (rGO). Especially, the energy densities of KI-, KBr-, and KCl-based materials are 722.2, 635.0, and 739.4 Wh kg-1 , respectively, which are higher than those of other cathode materials for potassium-ion batteries. In addition, the full-cell with 2D KI/rGO as cathode and graphite as anode demonstrates a lifespan of over 150 cycles with a considerable capacity retention of 57.5%. The metal halides-based electrode materials possess promising application prospects and are worthy of more in-depth researches.

6.
Biochem Biophys Res Commun ; 636(Pt 1): 170-177, 2022 Dec 25.
Artigo em Inglês | MEDLINE | ID: mdl-36334441

RESUMO

NSCs play an essential role in the regeneration process of the central nervous system. However, due to the influence of the harsh pathological microenvironment, the viability of neural stem cells is limited, and the therapeutic effect needs improvement. Previous studies have found that stem cells overexpressing ciliary neurotrophic factor (CNTF) have apparent therapeutic effects on remyelination, but the specific mechanism of action still needs to be further explored. We found that astrocytes, the most numerous groups in the CNS, exhibited a pathological role in the experimental autoimmune encephalomyelitis model, but after stimulation with CNTF-NSCs, a phenotypic switch occurred and induced the neurotrophic factor cardiotrophin-like cytokine 1 (Clcf1) production. Mechanistically, Clcf1 can significantly promote the differentiation of oligodendrocyte precursor cells (OPCs), and the advanced effect can attenuate by the Clcf1 antibody. Therefore, this study was conducted to investigate the pathway by which CNTF-NSCs exert their therapeutic effects by affecting astrocytes. It is expected to identify a potential therapeutic factor, Clcf1, for the treatment of demyelinating diseases.


Assuntos
Células-Tronco Neurais , Células Precursoras de Oligodendrócitos , Fator Neurotrófico Ciliar/farmacologia , Células Precursoras de Oligodendrócitos/metabolismo , Astrócitos/metabolismo , Diferenciação Celular , Células-Tronco Neurais/metabolismo , Oligodendroglia
7.
Phys Chem Chem Phys ; 2022 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-36383084

RESUMO

It is essential to find a kind of electrocatalyst for hydrogen evolution reduction (HER) comparable with a noble metal that has good conductivity and abundant active sites. Based on systematic searches by first-principles calculations, we discovered two-dimensional transition-metal nitrides, tetra-phase OsN2 and ReN2 monolayers, as potential HER electrocatalysts with superior thermodynamic and kinetic stability. They exhibited excellent catalytic activity due to the presence of multiple active sites with a density of 8 × 1015 site per cm2 and an overpotential close to 0. In addition, we also found that the synergistic effect of strain and coverage makes them have a good hydrogen evolution activity. The ΔGH of the OsN2 monolayer at 1% tensile strain under 3/4 hydrogen coverage is 0.02 eV, and that of ReN2 at 1/2 hydrogen coverage could decrease to 0.001 eV. Different from other common transition metal nitrides, we found that the active sites of OsN2 and ReN2 monolayers are both at nitrogen atoms, which could be further understood by the crystal orbital Hamiltonian population analysis between N and metal atoms. All these interesting findings not only provide new excellent candidates but also provide new insights into the mechanism of hydrogen evolution of nitrides.

8.
Inorg Chem ; 61(46): 18752-18758, 2022 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-36345055

RESUMO

Here, a hexanuclear Co6(µ3-OH)6 cluster-based metal-organic framework (MOF), [Co6(µ3-OH)6(BTB)2(bpy)3]n (JXNU-15) (bpy = 4,4'-bipyridine), with the 1,3,5-tri(4-carboxyphenyl)benzene (BTB3-) ligand was synthesized for the challenging propadiene/propylene separation. The combination of a large pore volume and a suitable pore environment boosts the significantly high propadiene (C3H4) uptake (311 cm3 g-1 at 298 K and 100 kPa) for JXNU-15. An amine-functionalized MOF of JXNU-15(NH2) was further obtained with the 1,3,5-tri(4-carboxyphenyl)benzene analogue of 3,3″-diamino-5'-(3-amino-4-carboxyphenyl)-[1,1':3',1″-terphenyl]-4,4″-dicarboxylic ligand. The comparative studies of propadiene/propylene(C3H4/C3H6) separation performance between isostructural JXNU-15 and JXNU-15(NH2) are provided. JXNU-15(NH2) exhibits an impressive C3H4 capacity at low pressures with 69.1 cm3 g-1 at 10 kPa, which is twice that of JXNU-15 under the same conditions. Moreover, the separation selectivity of JXNU-15(NH2) is 1.3-fold higher as compared to JXNU-15. JXNU-15(NH2) with enhanced C3H4/C3H6 separation performance was elegantly illustrated by gas separation experiments and theoretical simulations. This work presents an amine-functionalized strategy for the enhancement of the C3H4/C3H6 separation performance of MOF.

10.
Front Genet ; 13: 1010361, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36338988

RESUMO

Background: Parkinson's disease (PD) is a neurodegenerative disease commonly seen in the elderly. On the other hand, cuprotosis is a new copper-dependent type of cell death that can be observed in various diseases. Methods: This study aimed to identify potential novel biomarkers of Parkinson's disease by biomarker analysis and to explore immune cell infiltration during the onset of cuprotosis. Gene expression profiles were retrieved from the GEO database for the GSE8397, GSE7621, GSE20163, and GSE20186 datasets. Three machine learning algorithms: the least absolute shrinkage and selection operator (LASSO), random forest, and support vector machine-recursive feature elimination (SVM-RFE) were used to screen for signature genes for Parkinson's disease onset and cuprotosis-related genes (CRG). Immune cell infiltration was estimated by ssGSEA, and cuprotosis-related genes associated with immune cells and immune function were examined using spearman correlation analysis. Nomogram was created to validate the accuracy of these cuprotosis-related genes in predicting PD disease progression. Classification of Parkinson's specimens using consensus clustering methods. Result: Three PD datasets from the Gene Expression Omnibus (GEO) database were combined after eliminating batch effects. By ssGSEA, we identified three cuprotosis-related genes ATP7A, SLC31A1, and DBT associated with immune cells or immune function in PD and more accurate for the diagnosis of Parkinson's disease course. Patients could benefit clinically from a characteristic line graph based on these genes. Consistent clustering analysis identified two subtypes, with the C2 subtype exhibiting higher immune cell infiltration and immune function. Conclusion: In conclusion, our study reveals that several newly identified cuprotosis-related genes intervene in the progression of Parkinson's disease through immune cell infiltration.

11.
Sci Total Environ ; : 160481, 2022 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-36436625

RESUMO

Phosphate fertilizer can cause fluoride accumulation in the soil and excessive fluoride poses potential risks to soil and food crop safety. However, the effects of fertilization on soil and fruit fluoride accumulations through long-term positioning trials, especially in high-fertilizer application situations such as apple orchards, have received little attention. To close this knowledge gap, we conducted a field experiment to demonstrate the effects among five long-term (13-year) fertilizer treatments on fluoride accumulations at different soil depths and in the fruits of an apple orchard in Baishui, Shaanxi, China. The five treatments included no fertilization (CK); manure application (M); nitrogen and phosphate fertilization (NP); nitrogen, phosphorus, and potassium fertilization (NPK); and groundcover with nitrogen and phosphate fertilization (GNP). The results confirmed that all phosphate fertilizer treatments (NP, NPK, and GNP) had significant increases in total fluoride (TF) and water-soluble fluoride (WF) accumulations in the topsoil (0-20cm). Among the phosphate fertilizer treatments, NP topsoil accumulated the most TF and WF, 79.4 and 7.7 mg kg-1 more than CK, respectively, and an average annual TF increase of 6 mg kg-1. However, the M treatment both experienced reduced fluoride content in the topsoil and had TF and WF contents 8.1 and 0.67 mg kg-1 lower than CK, respectively. There was no significant difference between all fertilizer treatments and CK in apple fluoride content. Furthermore, TF and WF accumulated mainly in the topsoil and the differences in soil fluoride content among the treatments decreased with soil depth. These findings showed that long-term phosphate fertilization increases the risk of fluoride accumulation in orchard topsoil. Altogether, these results improve the understanding of the characterization of soil and fruit fluoride accumulations in apple orchards under long-term fertilizer conditions and provide new insights into the use of manure as a possible alternative fertilization strategy that reduces fluoride accumulation.

12.
BMJ Open ; 12(11): e063100, 2022 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-36428023

RESUMO

OBJECTIVES: The administration of nursing assistants (NAs) is closely associated with patient outcomes, but studies evaluating intrahospital administration of NAs are limited. This study aimed to identify existing literature on intrahospital NAs' administration approaches. DESIGN: Scoping review. DATA SOURCES: PubMed, Embase, CINAHL, Scopus, ProQuest, CNKI, APA PsycInfo, Wanfang Med, SinoMed, Ovid Emcare, NICE, AHRQ, CADTH, JBI EBP and Cochrane DSR were searched for articles published between January 2011 and March 2022. ELIGIBILITY CRITERIA FOR SELECTING STUDIES: Qualitative, quantitative or mixed-method studies and evidence syntheses that evaluate administration approaches, models and appraisal tools of intrahospital NAs were included. DATA EXTRACTION AND SYNTHESIS: Two independent reviewers conducted search, data selection and data extraction according to Joanna Briggs Institute guidance and methodology for scoping review. The quality of included studies was assessed using Mixed Methods Appraisal Tool or AMSTAR V.2. Data were synthesised using narrative methods and frequency effect size analysis. RESULTS: Thirty-six studies were eligible, with acceptable quality. We identified 1 administration model, 9 administration methods, 15 educational programmes and 7 appraisal tools from the included studies. The frequency effect size analysis yielded 15 topics of the main focus at four levels, suggesting that included articles were mainly (33%) focused on the competency of NAs, and the lectures were the most (80%) used strategy in quality improvement projects. Evidence from the studies was of low-to-moderate quality, indicating huge gaps between evidence-based research and management practice. CONCLUSIONS: Practical intrahospital administration approaches were revealed, and fifteen primarily focused topics were identified. We should explore this area more thoroughly using structured frameworks and standardised methodology. This scoping review will help managers find more effective ways to improve the quality of care. Researchers may focus more on evidence-based practice in NA administration using the 15 topics as a breakthrough.

13.
Fish Shellfish Immunol Rep ; 3: 100048, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36419606

RESUMO

The immunoglobulin (Ig) is a crucial component of adaptive immune system in vertebrates including teleost fish. Here complete cDNA sequence of IgD heavy chain gene from common carp (Cyprinus carpio) was cloned and analyzed. The full-length cDNA of IgD heavy chain gene contained an open reading frame (ORF) of 2460 bp encoding 813 amino acids. According to amino acids sequence, multiple alignment and phylogenetic analysis showed that carp Igs are closely related to those of Cyprinidae fish. Transcriptional expression of IgD as well as IgM, IgZ1 and IgZ2 showed similar expression patterns in different organs, this is, high expression level in systemic immune tissues (ie, head kidney, heart and spleen) and low expression in mucosal tissues (ie, gill, skin and gut). Following viral infection with spring viraemia of carp virus (SVCV), obvious pathological changes in skin, gill and gut mucosa and up-regulated expression of antiviral related genes in skin, gill, gut and spleen were observed, indicating that SVCV successfully infected common carp and activated the systemic and mucosal immune system. Interestingly, IgM showed a significant up-regulation only in systemic tissue (spleen), but not in mucosal tissues (gut, gills and skin), while increased expression of IgZ1 and IgZ2 was found in gut. In contrast, the expression of IgD increased significantly in spleen, gills and skin. These strongly suggest that fish Ig isotypes play different roles in mucosal and systemic immunity during viral infection. Common carp (Cyprinus carpio); Igs; Spring viraemia of carp virus (SVCV).

14.
Front Neurol ; 13: 937536, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36425805

RESUMO

Objective: The aim of this study was to investigate the efficacy and complications of stent-assisted coiling in comparison with flow diversion for wide-necked intracranial aneurysms. Methods: Patients with wide-necked intracranial aneurysms who were treated with stent-assisted coiling or flow diversion were respectively, enrolled into the stent-assisted coiling or flow diversion treatment group. The clinical and angiographic data were analyzed. Results: A total of 61 patients with intracranial aneurysms underwent stent-assisted coiling, including 35 (57.4%) female and 26 (42.6%) male patients with 21 (34.4%) ruptured and 40 (65.6%) unruptured aneurysms. Also, 53 patients underwent deployment of flow-diverting devices, including 30 (56.6%) female and 23 (43.4%) male patients with 25 (47.2%) ruptured and 28 (52.8%) unruptured aneurysms. Stent-assisted coiling was performed successfully in 60 patients with 63 stents deployed, and immediate aneurysm occlusion was complete occlusion in 38 (62.3%) aneurysms, residual neck in 12 (19.7%), and residual aneurysm in 10 (16.4%). Procedure-related complications included in-stent thrombosis in three (4.9%) patients, coil protrusion in three (4.9%), and re-rupture of one (1.6%) aneurysm, with a total complication rate of 11.5%. In the flow diversion group, a pipeline embolization device alone was deployed in each of the 24 (45.3%) patients, adjunctive coiling combined with a pipeline device in 29 (54.7%), and double pipeline devices in each of the 6 (11.3%) patients. Immediately after treatment, complete occlusion was achieved in 3 (5.7%) patients with adjunctive coiling, residual neck in 3 (5.7%), and residual aneurysm in 47 (88.7%). Procedure-related complications included aneurysm rebleeding in one patient (1.9%). Clinical and angiographic follow-up was performed 13-49 months (median 29) after the procedure for 49 (80.3%) patients with stent-assisted coiling, with complete aneurysm occlusion in 27 (55.1%) aneurysms, residual neck in 3 (6.1%), residual aneurysm in 5 (10.2%), and recurrence in 14 (28.6%). Follow-up was performed for 14-37 (median 25) months in 45 (84.9%) patients with flow diversion treatment, with complete occlusion in 39 (86.7%) patients, residual neck in 5 (11.1%), residual aneurysm in 1 (2.2%), and no aneurysm recurrence. Conclusions: Stent-assisted coiling comes with more complications but fewer permanent aneurysm occlusions than flow diverters, and flow diverters are superior to stent-assisted coiling in the treatment of wide-necked intracranial aneurysms, especially in the long-term effect.

15.
Dis Markers ; 2022: 2074356, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36246570

RESUMO

The development of calcific aortic valve disease (CAVD) is a complex process of ectopic calcification involving various factors that lead to aortic valve stenosis, hemodynamic changes, and, in severe cases, even sudden death. Currently, aortic valve replacement is the only effective method. The osteogenic differentiation of aortic valve interstitial cells (AVICs) is one of the key factors of valve calcification. Emerging evidence suggests that bone morphogenetic protein 2 (BMP2) can induce the proosteogenic activation of AVICs. However, the regulatory mechanism underlying this activation in AVICs is unclear. In the present study, we elucidated through high-throughput RNA sequencing and RT-qPCR that miR-664a-3p was evidently downregulated in the calcific aortic valve. We also proved that miR-664a-3p was involved in regulating osteogenic differentiation in AVICs. Target prediction analysis and dual-luciferase reporter gene assay confirmed that miR-664a-3p is preferentially bound to BMP2. Furthermore, the effect of the miR-664a-3p/BMP2 axis on osteogenic differentiation in AVICs was examined using the gain- and loss-of-function approach. Finally, we constructed a mouse CAVD model and verified the effect of the miR-664a-3p/BMP2 axis on the aortic valve calcification leaflets in vivo. In conclusion, miR-664a-3p regulates osteogenic differentiation in AVICs through negative regulation of BMP2, highlighting that miR-664a-3p may be a potential therapeutic target for CAVD.


Assuntos
Estenose da Valva Aórtica , MicroRNAs , Animais , Valva Aórtica/metabolismo , Valva Aórtica/patologia , Estenose da Valva Aórtica/genética , Estenose da Valva Aórtica/metabolismo , Proteína Morfogenética Óssea 2/genética , Proteína Morfogenética Óssea 2/metabolismo , Calcinose , Células Cultivadas , Camundongos , MicroRNAs/genética , MicroRNAs/metabolismo , Osteogênese/genética , Transdução de Sinais , Regulação para Cima
16.
Respir Res ; 23(1): 286, 2022 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-36253857

RESUMO

The role of micro RNAs (miRNAs) in asthma remains unclear. In this study, we examined the role of miRNA in targeting FOXO1 in asthma. Results showed that miR-493-5p was one of the differentially expressed miRNAs in the PBMCs of asthmatic children, and was also associated with Th cell differentiation. The miR-493-5p expression decreased significantly in the OVA-induced asthma mice than the control groups. The miR-493-5p mimic inhibited the expression of the IL-9, IRF4 and FOXO1, while the inhibitor restored these effects. Moreover, the Dual-Luciferase analysis results showed FOXO1 as a novel valid target of miR-493-5p. According to the rescue experiment, miR-493-5p inhibited Th9 cell differentiation by targeting FOXO1. Then the exosomes in association with the pathogenesis of asthma was identified. Various inflammatory cells implicated in asthmatic processes including B and T lymphocytes, DCs, mast cells, and epithelial cells can release exosomes. Our results demonstrated that the DC-derived exosomes can inhibit Th9 cell differentiation through miR-493-5p, thus DC-derived exosomal miR-493-5p/FOXO1/Th9 may serve as a potential therapeutic target in the development of asthma.


Assuntos
Asma , Proteína Forkhead Box O1 , MicroRNAs , Linfócitos T Auxiliares-Indutores , Animais , Camundongos , Asma/genética , Diferenciação Celular , Proteína Forkhead Box O1/genética , Proteína Forkhead Box O1/metabolismo , Interleucina-9/metabolismo , MicroRNAs/genética , Ovalbumina , Linfócitos T Auxiliares-Indutores/metabolismo
17.
ACS Appl Mater Interfaces ; 14(41): 46304-46312, 2022 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-36196653

RESUMO

Biomolecule-based piezoelectric nanostructures emerged as a new class of energy-converse materials, and designing tailored piezoelectric amino acid arrays is essential to achieve efficient electrical-mechanical coupling and fulfill their application potential. However, the controlled growth of amino acid nanostructures is still challenging due to the limited understanding of their growth mechanism. Herein, we base on the Stranski-Krastanov (S-K) growth mode and propose a mechanism for the growth of ordered amino acid array structures via physical vapor deposition. The growth of vertical valine sheet arrays is examined by changing the substrate temperature, chamber pressure, and source-substrate distance, and a "layer-plus-sheet" growth process is revealed. The modified S-K growth mode is applied to fabricate other amino acid nanostructures like leucine and isoleucine. The growth mode not only explains the formation of uniform and controllable morphology of amino acid structures but also leads to the significant enhancement of their piezoelectric properties. The maximal effective piezoelectric constant of valine sheets is 11.4 pm V-1, which approaches its highest predicted value. The output voltage of the valine array-based nanogenerator is ∼4.6 times the output voltage of the valine powder-based nanogenerator. This work provides new insights into the growth mechanism of ordered piezoelectric amino acid arrays, making them promising candidates for applications in wearable or implantable electronic devices.


Assuntos
Aminoácidos , Fontes de Energia Elétrica , Leucina , Isoleucina , Pós , Valina
18.
Nat Commun ; 13(1): 5903, 2022 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-36202850

RESUMO

Emerging functionalities in two-dimensional materials, such as ferromagnetism, superconductivity and ferroelectricity, open new avenues for promising nanoelectronic applications. Here, we report the discovery of intrinsic in-plane room-temperature ferroelectricity in two-dimensional Bi2TeO5 grown by chemical vapor deposition, where spontaneous polarization originates from Bi column displacements. We found an intercalated buffer layer consist of mixed Bi/Te column as 180° domain wall which enables facile polarized domain engineering, including continuously tunable domain width by pinning different concentration of buffer layers, and even ferroelectric-antiferroelectric phase transition when the polarization unit is pinned down to single atomic column. More interestingly, the intercalated Bi/Te buffer layer can interconvert to polarized Bi columns which end up with series terraced domain walls and unusual fan-shaped ferroelectric domain. The buffer layer induced size and shape tunable ferroelectric domain in two-dimensional Bi2TeO5 offer insights into the manipulation of functionalities in van der Waals materials for future nanoelectronics.

19.
Front Pharmacol ; 13: 1013485, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36204240

RESUMO

Objective: The present study aims to assess the willingness to pay (WTP) for and willingness to vaccinate (WTV) with the Coronavirus (COVID-19) vaccine booster dose in China when the pandemic is under adequate control and the majority of the population is vaccinated. This study is also to identify significant factors associated with the WTP. Methods: This was a cross-sectional study on adults with no past or present COVID-19 infection. An online questionnaire was distributed to collect data on vaccination status, quarantine experience, and factors related to health beliefs on vaccination. The WTV was assessed through the vaccination preference. The WTP was examined by payment scale (PS) and iterative bidding game (IBG) administered in random order. Three IBG algorithms with different starting-price were presented randomly. The average WTP of PS and IBG were analyzed as primary outcomes using univariate and multivariate analyses. Multivariate ordered logistic regression was performed to identify significant factors for the WTP. Results: The survey recruited 543 participants with a mean age of 32 years and 57.80% being female. The WTV rate was 86.74%, while 94.66% of participants completed full-schedule or enhanced vaccination. The mean WTP was CNY 149 (±CNY 197) and the median WTP was CNY 80. Regarding significant factors for the WTP, urban residents were 57% more likely (95% CI: 1.11-2.22) to pay for a high-priced vaccine than rural residents. Respondents who completed full-schedule vaccination were 46% more likely (95% CI: 1.03-2.07) to pay for a high-priced vaccine than those who completed enhanced vaccination. Respondents with a low household income of CNY 40k or lower were 62% less likely (95% CI: 0.21-0.66) to pay for a high-priced vaccine than those with a middle household income of CNY 110k-210k. Other significant factors associated with the WTP included the perceived benefit of vaccination and peer environmental pressure in the health belief model. Conclusion: The WTV with the COVID-19 vaccine booster dose was high in China. The WTP was influenced by the place of residence, vaccination status, household income, perceived benefit of vaccination, and environmental peer pressure. Study findings can inform policymakers to better design vaccination programs and financial schemes involving out-of-pocket payments.

20.
J Cancer Res Ther ; 18(5): 1372-1379, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-36204885

RESUMO

Delta and Notch-like endothelial growth factor-related receptor (DNER) is a transmembrane protein that mediates signal communication between neurons and glial cells. This study was performed to elucidate the specific mechanism by which DNER inhibits human glioma growth. RNA sequencing was used to detect differentially expressed genes after DNER inhibition in glioma cells. The functions of the Torsin family 4 member A (TOR4A) gene were explored through cell proliferation and clonogenic assays, flow cytometric analysis, in vitro cell migration and invasion assays, in vivo glioma transplantation, and human glioma tissue analysis using the Chinese Glioma Genome Atlas database. Protein expression levels were determined using the western blot assay. We found that TOR4A was highly expressed after the inhibition of DNER in glioma cells. The prognosis of patients with gliomas that expressed high levels of TOR4A was worse than those with low levels of the protein. TOR4A promoted the proliferation of glioma cells and inhibited their apoptosis, likely by enhancing the expression of phosphorylated protein kinase B (p-AKT) and inhibiting that of antiapoptotic proteins. We confirmed that TOR4A is an oncogene and that DNER acts as a tumor suppressor gene by inhibiting TOR4A and its functions of promoting p-AKT and inhibiting antiapoptotic protein expression.


Assuntos
Glioma , Proteínas Proto-Oncogênicas c-akt , Linhagem Celular Tumoral , Movimento Celular/genética , Proliferação de Células/genética , Família de Proteínas EGF/metabolismo , Fatores de Crescimento Endotelial/metabolismo , Regulação Neoplásica da Expressão Gênica , Glioma/patologia , Humanos , Oncogenes , Proteínas Proto-Oncogênicas c-akt/genética , Proteínas Proto-Oncogênicas c-akt/metabolismo
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