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1.
Artigo em Inglês | MEDLINE | ID: mdl-32396107

RESUMO

This article studies supervised video summarization by formulating it into a sequence-to-sequence learning framework, in which the input and output are sequences of original video frames and their predicted importance scores, respectively. Two critical issues are addressed in this article: short-term contextual attention insufficiency and distribution inconsistency. The former lies in the insufficiency of capturing the short-term contextual attention information within the video sequence itself since the existing approaches focus a lot on the long-term encoder-decoder attention. The latter refers to the distributions of predicted importance score sequence and the ground-truth sequence is inconsistent, which may lead to a suboptimal solution. To better mitigate the first issue, we incorporate a self-attention mechanism in the encoder to highlight the important keyframes in a short-term context. The proposed approach alongside the encoder-decoder attention constitutes our deep attentive models for video summarization. For the second one, we propose a distribution consistency learning method by employing a simple yet effective regularization loss term, which seeks a consistent distribution for the two sequences. Our final approach is dubbed as Attentive and Distribution consistent video Summarization (ADSum). Extensive experiments on benchmark data sets demonstrate the superiority of the proposed ADSum approach against state-of-the-art approaches.

2.
ACS Sens ; 2020 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-32375473

RESUMO

Ultrasensitive detection of sequence-specific DNA and uracil-DNA glycosylase (UDG) activity shows great practical significance in clinical diagnostic and biomedical studies. Here, a methodology based on a CRISPR/Cas12a system coupled with enhanced strand displacement amplification (E-SDA) was innovatively established for sequence-specific DNA or UDG activity detection. Sequence-specific DNA or DNA primers processed by UDG and Endonuclease IV can initiate E-SDA, generating auxiliary DNA chains, which act as activators to unlock the indiscriminate collateral cleavage activities (trans-cleavage) of the CRISPR/Cas12a. Then, the activated CRISPR/Cas12a, which intrinsically possesses the ability of significant signal amplification, can indiscriminately cleave the added cleavage reporters in the system. Thus, the multistep amplification of the method was obtained. Under the selected experimental conditions, the established method can achieve an actual sensitivity of sequence-specific DNA up to 100 aM within 2.5 h or ultralow UDG activity (3.1×10-5 U/mL) detection within 3.5 h. We believe that the proposed method will have great potential for practical application in ultrasensitive detection of sequence-specific DNA or UDG activity.

3.
IEEE Trans Cybern ; 2020 May 04.
Artigo em Inglês | MEDLINE | ID: mdl-32386178

RESUMO

This article focuses on tackling the task of the cross-modal image-text retrieval which has been an interdisciplinary topic in both computer vision and natural language processing communities. Existing global representation alignment-based methods fail to pinpoint the semantically meaningful portion of images and texts, while the local representation alignment schemes suffer from the huge computational burden for aggregating the similarity of visual fragments and textual words exhaustively. In this article, we propose a stacked multimodal attention network (SMAN) that makes use of the stacked multimodal attention mechanism to exploit the fine-grained interdependencies between image and text, thereby mapping the aggregation of attentive fragments into a common space for measuring cross-modal similarity. Specifically, we sequentially employ intramodal information and multimodal information as guidance to perform multiple-step attention reasoning so that the fine-grained correlation between image and text can be modeled. As a consequence, we are capable of discovering the semantically meaningful visual regions or words in a sentence which contributes to measuring the cross-modal similarity in a more precise manner. Moreover, we present a novel bidirectional ranking loss that enforces the distance among pairwise multimodal instances to be closer. Doing so allows us to make full use of pairwise supervised information to preserve the manifold structure of heterogeneous pairwise data. Extensive experiments on two benchmark datasets demonstrate that our SMAN consistently yields competitive performance compared to state-of-the-art methods.

4.
BMC Neurol ; 20(1): 189, 2020 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-32414382

RESUMO

BACKGROUND: Endovascular therapy (EVT) is increasingly used to improve cerebral reperfusion after moderate-to-severe acute ischemic stroke (AIS). However, the influence of hemodynamic factors on clinical outcome is still unclear after EVT. Dynamic cerebral autoregulation (dCA) is an important brain reserve mechanism and is impaired after AIS. This study aimed to explore the role of dCA in predicting the outcome of AIS patients after EVT. METHODS: AIS patients with severe stenosis/occlusion of unilateral middle cerebral artery (MCA) or internal carotid and treatment with EVT were enrolled to receive dCA examinations at the 24 h, 72 h and 7th day after stroke onset. Healthy volunteers were also recruited as controls. DCA was recorded from spontaneous fluctuations of blood pressure and MCA flow velocity. Transfer function analysis was used to derive dCA parameters, including phase difference (PD) and coherence in the low-frequency range (0.06-0.12 Hz). The clinical outcome was measured using the modified Rankin Scale (mRS) at 90 days after onset. Multivariate logistic regression was performed to reveal the correlation between dCA and clinical outcomes. The receiver operation characteristics (ROC) curve was performed to determine the cut-off point of PD. RESULTS: A total of 62 AIS patients and 77 healthy controls were included. Compared with controls, dCA were impaired bilaterally till to 7th day after onset in patients, presenting as much lower PD value on the ipsilateral side. During follow-up, we found that PD on the ipsilateral side at 24 h after onset was significantly lower in patients with unfavourable outcome (n = 41) than those with favourable outcome (n = 21), even after adjustment of confounding factors (p = 0.009). ROC curve analysis revealed that PD < 26.93° was an independent predictor of unfavourable-outcome. CONCLUSION: In AIS patients after EVT, dCA was impaired on both sides over the first 7 days. PD on the ipsilateral side at 24 h after onset is an independent unfavourable-outcome predictor for AIS after EVT.

5.
Artigo em Inglês | MEDLINE | ID: mdl-32406834

RESUMO

During the past decade, both multi-label learning and zero-shot learning have attracted huge research attention, and significant progress has been made. Multi-label learning algorithms aim to predict multiple labels given one instance, while most existing zero-shot learning approaches target at predicting a single testing label for each unseen class via transferring knowledge from auxiliary seen classes to target unseen classes. However, relatively less effort has been made on predicting multiple labels in the zero-shot setting, which is nevertheless a quite challenging task. In this work, we investigate and formalize a flexible framework consisting of two components, i.e., visual-semantic embedding and zero-shot multi-label prediction. First, we present a deep regression model to project the visual features into the semantic space, which explicitly exploits the correlations in the intermediate semantic layer of word vectors and makes label prediction possible. Then, we formulate the label prediction problem as a pairwise one and employ Ranking SVM to seek the unique multi-label correlations in the embedding space. Furthermore, we provide a transductive multi-label zeroshot prediction approach that exploits the testing data manifold structure. We demonstrate the effectiveness of the proposed approach on three popular multi-label datasets with state-of-theart performance obtained on both conventional and generalized ZSL settings.

6.
Mikrochim Acta ; 187(6): 341, 2020 May 22.
Artigo em Inglês | MEDLINE | ID: mdl-32444888

RESUMO

N-Doped silicon quantum dots (N-SiQD) were synthesized using N-[3-(trimethoxysily)propyl]-ethylenediamine and citric acid as silicon source and reduction agent, respectively. The N-SiQD shows a strong blue fluorescence with a high quantum yield of about 53%. It is found that a selective static quenching process occurs between N-SiQDs and Cu2+. Glyphosate can inhibit this phenomenon and trigger the rapid fluorescence enhancement of the quenched N-SiQDs/Cu2+ system due to the specific interaction between Cu2+ and glyphosate. With such a design, a turn-on fluorescent nanoprobe based on N-SiQD/Cu2+ system was established for rapid determination of glyphosate. The determination signal of N-SiQD/Cu2+ was measured at the optimum emission wavelength of 460 nm after excitation at 360 nm. Under optimal conditions, the turn-on nanoprobe showed a linear relationship between fluorescent response and glyphosate concentrations in the range 0.1 to 1 µg mL-1. The limit of determination was calculated to 7.8 ng mL-1 (3σ/S). Satisfactory recoveries were obtained in the determination of spiked water samples, indicating the potential use for environmental monitoring. Graphical abstract Schematic representation of N-SiQD/Cu2+ system for glyphosate determination. Fluorescence quenching of N-SiQDs induced by copper ions and the succedent fluorescent "turn on" triggered by glyphosate.

7.
Zhongguo Zhong Yao Za Zhi ; 45(6): 1357-1362, 2020 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-32281348

RESUMO

The aim of this paper was to evaluate the key production processes of Schizonepetae Herba formula granules based on the new model of combining characteristic chromatogram with quantitative transfer relationship. The rationality of production process design was evaluated by studying the intermediates in different processes of formula granules, analyzing the loss of index component pulegone in each step, and establishing the characteristic chromatogram. The content of pulegone in 10 batches of standard decoction ranged between 0.067% and 0.124%(70%-130% of the average value), and the transfer rate of pulegone was 44.58%-93.97%. After the improvement of the production process, the content of pulegone in Schizonepetae Herba formula granules was 0.093%, and the transfer rate of pulegone was 68.38%, which was consistent with the parameters range of standard decoction. This study emphasized the integrality of the research process of traditional Chinese medicine(TCM) formula granules, and provided a new idea for the quality control of TCM with content determination as the main evaluation index for a long time.


Assuntos
Medicamentos de Ervas Chinesas/química , Lamiaceae/química , Controle de Qualidade , Cromatografia Líquida de Alta Pressão , Medicina Tradicional Chinesa
8.
Nat Commun ; 11(1): 1685, 2020 Apr 03.
Artigo em Inglês | MEDLINE | ID: mdl-32245947

RESUMO

N6-methyladenosine (m6A) is the most prevalent modification in eukaryotic RNAs. The biological importance of m6A relies on m6A readers, which control mRNA fate and function. However, it remains unexplored whether additional regulatory subunits of m6A readers are involved in the m6A recognition on RNAs. Here we discover that the long noncoding RNA (lncRNA) LINC00266-1 encodes a 71-amino acid peptide. The peptide mainly interacts with the RNA-binding proteins, including the m6A reader IGF2BP1, and is thus named "RNA-binding regulatory peptide" (RBRP). RBRP binds to IGF2BP1 and strengthens m6A recognition by IGF2BP1 on RNAs, such as c-Myc mRNA, to increase the mRNA stability and expression of c-Myc, thereby promoting tumorigenesis. Cancer patients with RBRPhigh have a poor prognosis. Thus, the oncopeptide RBRP encoded by LINC00266-1 is a regulatory subunit of m6A readers and strengthens m6A recognition on the target RNAs by the m6A reader to exert its oncogenic functions.

9.
Anal Chim Acta ; 1108: 152-159, 2020 Apr 29.
Artigo em Inglês | MEDLINE | ID: mdl-32222236

RESUMO

In this study, direct detection of fluazinam was realized using a fluorescent sensor using disulfide quantum dots (MoS2 QDs) via inner filter effect (IFE). The maximum excitation of as-prepared MoS2 QDs presented a complementary spectral-overlap with the maximum absorption of fluazinam. Thus the occurrence of inner filter effect led to the significant fluorescence quenching of MoS2 QDs. Additionally, fluorescent quenching efficiency of MoS2 QDs could be enhanced by the effects of π-π stacking, hydrogen bond and electrostatic interaction between fluazinam and MoS2 QDs, and these non-chemical bond responses also promoted the selectivity for fluazinam detection. Under the optimum conditions, the IFE-based fluorescent sensor exhibited a relative wide linear range from 50 nM to 25 µM with the LOD of 2.53 nM (S/N = 3). In addition, a paper-based sensor was established by cross-linking the MoS2 QDs into cellulose membrane for naked-eyed detection and digital analysis of fluazinam. The paper-based sensor presented a liner range from 10 µM to 800 µM for fluazinam detection with the LOD of 2.26 µM. Additionally, the acceptable recoveries were obtained for fluazinam detection in the spiked samples of tomato, potato and cucumber, indicating that the proposed method provided an effective sensing platform for real applications of fluazinam detection in food safety.

10.
Biomed Res Int ; 2020: 1283214, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32076604

RESUMO

Bupivacaine, a common local anesthetic, causes serious nerve injury, especially in diabetic patients, as high glucose has been reported to enhance bupivacaine-induced neurotoxicity. However, the key regulator for synergism remains unknown. To our surprise, the expression of repair protein Ku70 is suppressed, while the high-glucose environment induces DNA oxidative damage in neurons. Here, we aim to investigate whether the inhibition of Ku70 by high-glucose conditions aggrandized bupivacaine-induced DNA damage. Consistent with previous results, bupivacaine induced reactive oxygen species production and upregulated Ku70 and cleaved caspase-3 expressions at both transcript and protein levels and ultimately caused nucleic acid damage and apoptosis in human neuroblastoma (SH-SY5Y) cells. High-glucose treatment inhibited the expression of Ku70 and enhanced bupivacaine-induced neurotoxicity. In contrast, the overexpression of Ku70 mitigated DNA damage and apoptosis triggered by bupivacaine and high glucose. In conclusion, our data indicated that local anesthetics may aggravate nerve toxicity in a high-glucose environment.

11.
Eur J Surg Oncol ; 46(4 Pt A): 600-606, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31973925

RESUMO

OBJECTIVE: Cytoreductive surgery (CRS) and hyperthermic intraperitoneal chemotherapy (HIPEC) is a standard treatment for pseudomyxoma peritonei (PMP) recommended by Peritoneal Surface Oncology Group International (PSOGI). The study is to analyze the incidence of perioperative serious adverse events (SAEs) of CRS + HIPEC to treat PMP patients, and identify the risk factors, for guiding the prevention of SAEs. METHODS: This is a retrospective study on the PMP database established at our center. The clinicopathological features, treatment details and SAEs information on the PMP patients are systematically established in this database. The incidence, organ system distribution and severity of perioperative SAEs are analyzed. Univariate and multivariate analyses are performed to identify the independent risk factors. RESULTS: Among the 272 CRS + HIPEC procedures for 254 PMP patients, there are 93 (34.2%) SAEs. Six systems are involved in the SAEs, including infections (9.6%), digestive system (8.1%), respiratory system (6.3%), cardiovascular system (5.5%), hematological system (2.9%), and urinary system (1.5%), in terms of frequency. In terms of severity, the majority is grade III SAEs (27.9%), followed by grade IV SAEs (4.8%) and grade V SAEs (1.5%). Univariate analysis reveals 4 risk factors for perioperative SAEs: HIPEC regimens (P = 0.020), PCI (P = 0.025), intraoperative red blood cell transfusion volume (P = 0.004), and intraoperative blood loss volume (P = 0.002). Multivariate and logistic regression model analysis identifies only one independent risk factor for perioperative SAEs: intraoperative blood loss volume (P = 0.001, OR = 0.344, 95%CI: 0.182-0.649). CONCLUSIONS: PMP patients treated by CRS + HIPEC at experienced centers could have acceptable safety. Improving the surgical techniques and developing the integrated hemostasis techniques are essential to reduce intraoperative blood loss and decrease SAEs rate.

12.
IEEE Trans Neural Netw Learn Syst ; 31(1): 321-330, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30990194

RESUMO

Zero-shot hashing (ZSH) aims at learning a hashing model that is trained only by instances from seen categories but can generate well to those of unseen categories. Typically, it is achieved by utilizing a semantic embedding space to transfer knowledge from seen domain to unseen domain. Existing efforts mainly focus on single-modal retrieval task, especially image-based image retrieval (IBIR). However, as a highlighted research topic in the field of hashing, cross-modal retrieval is more common in real-world applications. To address the cross-modal ZSH (CMZSH) retrieval task, we propose a novel attribute-guided network (AgNet), which can perform not only IBIR but also text-based image retrieval (TBIR). In particular, AgNet aligns different modal data into a semantically rich attribute space, which bridges the gap caused by modality heterogeneity and zero-shot setting. We also design an effective strategy that exploits the attribute to guide the generation of hash codes for image and text within the same network. Extensive experimental results on three benchmark data sets (AwA, SUN, and ImageNet) demonstrate the superiority of AgNet on both cross-modal and single-modal zero-shot image retrieval tasks.

13.
Per Med ; 17(1): 23-31, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31797720

RESUMO

Aim: The dose of digoxin is often difficult to be determined precisely. The aim of this study was to retrospectively investigate the effect of blood biochemical indexes on the serum concentration of digoxin. Materials & methods: We collected the data of hospitalized patients treated orally with digoxin in Nanjing Drum Tower Hospital (Nanjing, China) from 2016 to 2018. Descriptive statistics was used to analyze the patients' comprehensive condition. Results: A total of 425 patients were included in the study. Through analysis, nine factors were included in the regression model of the serum concentration of digoxin, and this regression model showed good predictive performance (r2 = 0.83138; p < 0.001). Conclusion: The regression model for the prediction of serum concentration of digoxin has clinical significance, and can provide research basis for individualized medication of digoxin.

14.
Nan Fang Yi Ke Da Xue Xue Bao ; 39(10): 1232-1238, 2019 Oct 30.
Artigo em Chinês | MEDLINE | ID: mdl-31801723

RESUMO

OBJECTIVE: To investigate the effect of ulinastatin on the inflammatory mediators and their signaling pathways miR-146a/TLR4/NF-κB in rats with hemorrhagic shock. METHODS: Seventy-two SD rats were randomly assigned into shock without resuscitation group (SR group, n=24), acetated Ringer's solution resuscitation group (AR group, n=24) and ulinastatin treatment group (n=24). In all the 3 groups hemorrhagic shock models were established by femoral artery bleeding (with the mean arterial pressure maintained at 30-40 mmHg) without resuscitation (in SR group) or with resuscitation (in AR and ulinastatin groups) using acetated Ringer's solution for 30 min at 60 min after the onset of shock. At 1, 4, and 6 h after the shock onset or immediately after shock if the rats died, the lung tissues were taken for measurement of mRNA expressions of miR-146a, tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1), IL-4, IL-6 and IL-10 using real-time quantitative PCR and the protein expressions of TLR4, MyD88, IκB-α, p-IκB-α, NF-κB p65, IRAK4, p-IRAK4 (Thr345, Ser346), p-IRAK4 (Thr342) and TRAF6 using Western blotting. The lung histopathology of the rats was examined under optical microscope with HE staining. RESULTS: Compared with the SR group, the rats in the AR group showed slightly alleviated inflammatory infiltration in the lung tissues with significantly increased mRNA levels of miR-146a, IL-4 and IL-10 (P < 0.05) and protein expressions of IκB-α, p-IRAK4 (Thr342) and p-IRAK4 (Thr345, ser346) (P < 0.05), and decreased mRNA levels of TNF-α, IL-1 and IL-6 (P < 0.05) and protein expressions of TLR4, MyD88, NF-κB p65, p-IκB-α, IRAK-4 and TRAF6 (P < 0.05). Compared with those in AR group, the rats in ulinastatin group showed further alleviation of inflammatory lung tissue injury, with increased mRNA levels of miR-146a, IL-4 and IL-10 (P < 0.01) and protein expressions of IκB-α, p-IRAK4 and p-IRAK4 (P < 0.01) and decreased mRNA levels of TNF-α, IL-1 and IL-6 (P < 0.01) and protein expressions of TLR4, MyD88, NF-κB p65, p-IκB-α, IRAK-4 and TRAF6 (P < 0.01). CONCLUSIONS: Ulinastatin combined with acetated Ringer's solution resuscitation alleviates lung inflammations in rats with hemorrhagic shock possibly by enhancing miR-146a expression to regulate TLR4/NF-κB signaling pathway through a negative feedback mechanism and thus modulate the balance of pro-inflammatory and anti-inflammatory factors.


Assuntos
Glicoproteínas/farmacologia , Inflamação/tratamento farmacológico , Pulmão/patologia , Choque Hemorrágico/tratamento farmacológico , Transdução de Sinais , Animais , Citocinas/metabolismo , Pulmão/efeitos dos fármacos , MicroRNAs/genética , NF-kappa B/metabolismo , Distribuição Aleatória , Ratos , Ratos Sprague-Dawley , Choque Hemorrágico/complicações , Receptor 4 Toll-Like/metabolismo
15.
Sensors (Basel) ; 19(24)2019 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-31847474

RESUMO

Blood pressure is an extremely important blood hemodynamic parameter. The pulse wave contains abundant blood-pressure information, and the convenience and non-invasivity of its measurement make it ideal for non-invasive continuous monitoring of blood pressure. Based on combined photoplethysmography and electrocardiogram signals, this study aimed to extract the waveform information, introduce individual characteristics, and construct systolic and diastolic blood-pressure (SBP and DBP) estimation models using the back-propagation error (BP) neural network. During the model construction process, the mean impact value method was employed to investigate the impact of each feature on the model output and reduce feature redundancy. Moreover, the multiple population genetic algorithm was applied to optimize the BP neural network and determine the initial weights and threshold of the network. Finally, the models were integrated for further optimization to generate the final individualized continuous blood-pressure monitoring models. The results showed that the predicted values of the model in this study correlated significantly with the measured values of the electronic sphygmomanometer. The estimation errors of the model met the Association for the Advancement of Medical Instrumentation (AAMI) criteria (the SBP error was 2.5909 ± 3.4148 mmHg, and the DBP error was 2.6890 ± 3.3117 mmHg) and the Grade A British Hypertension Society criteria.


Assuntos
Pressão Sanguínea/fisiologia , Eletrocardiografia/métodos , Fotopletismografia/métodos , Algoritmos , Determinação da Pressão Arterial/métodos , Humanos
16.
Zhongguo Zhong Yao Za Zhi ; 44(21): 4641-4647, 2019 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-31872659

RESUMO

This study aimed to establish characteristic chromatogram and content determination method for Chan Taoren formula granules,evaluate the production processes of Chan Taoren formula granules based on the correlation of characteristic chromatogram and the transfer rate of D-amygdalin,and clarify the key control points. The optimized analytical method was carried out on a Waters CORTECS C18 column(4. 6 mm×150 mm,2. 7 µm) with acetonitrile-0. 1% phosphoric acid aqueous solution as the mobile phase at a flow rate of 0. 6 m L·min-1. The detection wavelength was 207 nm,and the column temperature was 20 ℃ . As compared with the standard decoction of Chan Taoren,there were five characteristic peaks in the decoction pieces,extracts,concentrates,spray-dried powders and formula granules,basically consistent in relative retention time and peak pattern; in addition,the transfer rate of D-amygdalin from Chan Taoren pieces to the formula granules was within the transfer rate range of standard decoction. The average transfer rate of D-amygdalin was 56.65%,72.85%,94.58% and 99.29% respectively in the extraction,concentration,spray drying and granulation processes. Therefore,the factors affecting D-amygdalin in the extraction process were further studied. The results showed that D-amygdalin was easily converted to L-amygdalin in the water extraction process,leading to a low transfer rate of D-amygdalin in this process.D-amygdalin was unstable under alkaline conditions and prone to isomerization. Both liquid to solid ratio and extraction time had significant effects on the extraction rate of D-amygdalin. In this study,the key links in the production process of Chan Taoren formula granules was clarified based on the characteristic chromatogram and the quantity transmission of D-amygdalin,which provided a theoretical basis for production and quality control.


Assuntos
Amigdalina , Medicamentos de Ervas Chinesas , Cromatografia Líquida de Alta Pressão , Controle de Qualidade , Água
17.
Zhongguo Zhong Yao Za Zhi ; 44(19): 4158-4164, 2019 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-31872693

RESUMO

Insulin resistance,as the main link in the pathogenesis of type 2 diabetes mellitus( T2 DM),runs through the whole process of occurrence and development of T2 DM and is closely related to the insulin receptor signaling pathway. Insulin stimulation causes autophosphorylation of the insulin receptor( IR),which then activates tyrosine phosphorylation of insulin receptor substrate( IRS).Phosphorylation of IRS can induce and activate phosphatidylinositol 3-kinase( PI3 K),subsequently activate downstream 3-phosphoinositide-dependent protease 1( PDK1) and Akt/PKB,and finally promote expression and translocation of glucose transporter 4 to increase glucose uptake of insulin-sensitive tissues and alleviate insulin resistance. Currently,oral hypoglycemic agents for clinical treatment of T2 DM have different side effects on the human body. Traditional Chinese medicine not only has a wide range of sources and abundant types,but also has comprehensive multi-component,multi-link and multi-target effects,showing unique advantages in the treatment of diabetes. In recent years,more and more researchers at home and abroad pay attention to the active ingredients in traditional Chinese medicine for alleviating insulin resistance. In this paper,we would summarize the active hypoglycemic ingredients of traditional Chinese medicine associated with the insulin receptor signaling pathway,which may provide some theoretical guidance for the development of traditional Chinese medicine in the treatment of diabetes.


Assuntos
Hipoglicemiantes/uso terapêutico , Resistência à Insulina , Medicina Tradicional Chinesa , Receptor de Insulina/metabolismo , Diabetes Mellitus Tipo 2 , Humanos , Insulina , Proteínas Substratos do Receptor de Insulina , Fosfatidilinositol 3-Quinases , Fosforilação , Proteínas Proto-Oncogênicas c-akt , Transdução de Sinais
18.
Clin Appl Thromb Hemost ; 25: 1076029619890415, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31775523

RESUMO

BACKGROUND: This study was to assess the risk of venous thromboembolism (VTE) in patients with peritoneal carcinomatosis (PC) and to evaluate the safety and feasibility of physiotherapy program to prevent VTE during cytoreductive surgery (CRS) and hyperthermic intraperitoneal chemotherapy (HIPEC). METHODS: For VTE prevention, we developed a systematic physiotherapy program consisting of active exercises of both arms and legs, and intermittent pneumatic compression device to massage both legs. This physiotherapy was applied to all patients, and the VTE-related events were recorded and analyzed. RESULTS: Cytoreductive surgery + HIPEC was performed on 466 patients with PC. All patients had highest VTE risk, with the median Caprini risk factor score being 11. During the 3-month observation period, 8 patients had 9 (1.9%) clinically symptomatic VTE events, including 8 (1.7%) deep vein thrombosis and 1 (0.2%) pulmonary embolism. Among those, 5 patients received pharmacological treatments with low-molecular-weight heparin, and the other 3 received physical exercises only. All these patients recovered well, and there was no mortality about VTE perioperatively. CONCLUSIONS: Patients with PC treated by CRS + HIPEC are at highest risk for VTE. The systematic physiotherapy program is safe and feasible to prevent VTE post CRS + HIPEC.

19.
Parasit Vectors ; 12(1): 464, 2019 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-31585545

RESUMO

BACKGROUND: Bluetongue disease of ruminants is a typical insect-borne disease caused by bluetongue virus (BTV) of the genus Orbivirus (family Reoviridae) and transmitted by some species of Culicoides (Diptera: Ceratopogonidae). Recently, the detection of BTV in yaks in high altitude meadows of the Shangri-La district of Yunnan Province, China, prompted an investigation of the Culicoides fauna as potential vectors of BTV. METHODS: A total of 806 Culicoides midges were collected by light trapping at three sites at altitudes ranging from 1800 to 3300 m. The species were identified based on morphology and the DNA sequences of cytochrome c oxidase subunit 1 (cox1). PCR and quantitative PCR following reverse transcription were used to test for the presence of BTV RNA in Culicoides spp. A phylogenetic analysis was used to analyze the cox1 sequences of some specimens. RESULTS: Four species dominated these collections and cox1 barcoding revealed that at least two of these appear to belong to species new to science. Culicoides tainanus and a cryptic species morphologically similar to C. tainanus dominated low altitude valley collections while C. nielamensis was the most abundant species in the high-altitude meadow. A species related to C. obsoletus occurred at all altitudes but did not dominate any of the collections. BTV RT-qPCR analysis detected BTV RNA in two specimens of C. tainanus, in one specimen closely related to C. tainanus and in one specimen closely related to C. obsoletus by barcode sequencing. CONCLUSIONS: This study suggests that BTV in high altitude areas of Yunnan is being transmitted by three species of Culicoides, two of which appear to be new to science. This research may be useful in improving understanding of the effects of global warming on arboviral disease epidemiology and further study is important in research into disease control and prevention.


Assuntos
Bluetongue/transmissão , Doenças dos Bovinos/transmissão , Ceratopogonidae/virologia , Insetos Vetores/virologia , Altitude , Animais , Sequência de Bases , Bluetongue/epidemiologia , Vírus Bluetongue/classificação , Vírus Bluetongue/genética , Vírus Bluetongue/isolamento & purificação , Bovinos , Doenças dos Bovinos/epidemiologia , Doenças dos Bovinos/virologia , Ceratopogonidae/classificação , China/epidemiologia , Código de Barras de DNA Taxonômico/veterinária , DNA Viral/química , DNA Viral/isolamento & purificação , Complexo IV da Cadeia de Transporte de Elétrons/genética , Cabras , Insetos Vetores/classificação , Filogenia , RNA Viral/isolamento & purificação , Reação em Cadeia da Polimerase em Tempo Real/veterinária , Transcrição Reversa , Ruminantes , Estudos Soroepidemiológicos
20.
Minerva Anestesiol ; 85(12): 1299-1307, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31486619

RESUMO

BACKGROUND: The C-reactive protein (CRP)/albumin ratio has been regarded as an outcome predictor in patients with cancer and sepsis. In this study, we evaluated the prognostic value of the CRP/albumin ratio in neurocritically ill patients. METHODS: We retrospectively reviewed consecutive patients admitted to the neurocritical care unit (NCU) of a university-affiliated hospital, between January 2013 and January 2017. The CRP/albumin ratio was calculated by dividing the CRP level by the albumin concentration that was obtained at NCU admission. Univariable and multivariable logistic regression were used to identify risk factors for 30-day unfavorable outcome (modified Rankin Scale of 4 to 6). The performance of the CRP/albumin ratio was further assessed by receiver operating characteristic (ROC) curve analysis. RESULTS: Of the 701 patients included, 398 (56.8%) had unfavorable outcome at 30 days. The median (interquartile range) value of the CRP/albumin ratio was noticeably higher in the unfavorable outcome group (0.9 [0.2-2.5]) than that in the favorable group (0.3 [0.1-1.2]). After adjustment for established prognostic markers of outcome, the CRP/albumin ratio (OR 1.205; 95% CI: 1.082-1.344) remained significant in predicting 30-day unfavorable outcome, with an area under the ROC curve of 0.644 and a cutoff value of 0.58. Further exploration showed that the prognostic value of the CRP/albumin ratio existed mainly in patients suffering septic shock (OR 1.616; 95% CI: 1.167-2.236), with an area under the ROC curve of 0.672 and a cutoff value of 1.75. CONCLUSIONS: A high CRP/albumin ratio at NCU admission was an independent predictor of 30-day unfavorable outcome in neurocritically ill patients, especially in those suffering septic shock.

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