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1.
J Vet Med Sci ; 83(10): 1608-1619, 2021 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-34470981

RESUMO

A novel avian infectious bronchitis virus (IBV) variant, designated as GX-NN160421, was isolated from vaccinated chicken in Guangxi, China, in 2016. Based on analysis of the S1 gene sequence, GX-NN160421 belonged to the New-type 1 (GVI-1) strain. More importantly, three consecutive nucleotides (AAC) deletions were found in the highly conserved structure gene N. The serotype of GX-NN160421 was different from those of the commonly used vaccine strains. The mortality of the GX-NN160421 strain was 3.33%, which contrasted with 50% mortality in the clinical case, but high levels of virus shedding lasted at least 21 days. In conclusion, the first novel IBV variant with three-nucleotide-deletion in the N gene was identified, and this unique variant is low virulent but with a long time of virus shedding, indicating the continuing evolution of IBV and emphasizing the importance of limiting exposure to novel IBV strains as well as extensive monitoring of new IBVs.


Assuntos
Infecções por Coronavirus , Vírus da Bronquite Infecciosa , Doenças das Aves Domésticas , Animais , Galinhas , China/epidemiologia , Infecções por Coronavirus/epidemiologia , Infecções por Coronavirus/veterinária , Genótipo , Vírus da Bronquite Infecciosa/genética , Nucleocapsídeo , Nucleotídeos , Filogenia , Doenças das Aves Domésticas/epidemiologia
2.
Biomed Res Int ; 2020: 1283214, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32076604

RESUMO

Bupivacaine, a common local anesthetic, causes serious nerve injury, especially in diabetic patients, as high glucose has been reported to enhance bupivacaine-induced neurotoxicity. However, the key regulator for synergism remains unknown. To our surprise, the expression of repair protein Ku70 is suppressed, while the high-glucose environment induces DNA oxidative damage in neurons. Here, we aim to investigate whether the inhibition of Ku70 by high-glucose conditions aggrandized bupivacaine-induced DNA damage. Consistent with previous results, bupivacaine induced reactive oxygen species production and upregulated Ku70 and cleaved caspase-3 expressions at both transcript and protein levels and ultimately caused nucleic acid damage and apoptosis in human neuroblastoma (SH-SY5Y) cells. High-glucose treatment inhibited the expression of Ku70 and enhanced bupivacaine-induced neurotoxicity. In contrast, the overexpression of Ku70 mitigated DNA damage and apoptosis triggered by bupivacaine and high glucose. In conclusion, our data indicated that local anesthetics may aggravate nerve toxicity in a high-glucose environment.


Assuntos
Bupivacaína/toxicidade , Dano ao DNA/efeitos dos fármacos , Reparo do DNA/efeitos dos fármacos , Glucose/metabolismo , Autoantígeno Ku/efeitos dos fármacos , Autoantígeno Ku/metabolismo , Síndromes Neurotóxicas/metabolismo , Apoptose , Caspase 3/metabolismo , Linhagem Celular Tumoral , Sobrevivência Celular , Regulação Neoplásica da Expressão Gênica , Células HEK293 , Humanos , Autoantígeno Ku/genética , Estresse Oxidativo/efeitos dos fármacos , Espécies Reativas de Oxigênio/metabolismo
3.
Anesth Analg ; 125(2): 653-661, 2017 08.
Artigo em Inglês | MEDLINE | ID: mdl-28682956

RESUMO

BACKGROUND: Increased oxidative stress has been linked to local anesthetic-induced nerve injury in a diabetic neuropathy (DN) rat model. The current study explores the effects of diphenyleneiodonium (DPI) chloride, an NADPH oxidase (NOX) inhibitor, on bupivacaine-induced sciatic nerve injury in DN rats. METHODS: A rat DN model was established through high-fat diet feeding and streptozotocin injection. The model was confirmed via testing (i) blood glucose, (ii) hindpaw allodynia responses to von Frey (VF) monofilaments, (iii) paw withdrawal thermal latency (PWTL), and (iv) nerve conduction velocity (NCV). Bupivacaine (Bup, 0.2 mL, 5 mg/mL) was used to block the right sciatic nerve. DPI (1 mg/kg) was injected subcutaneously 24 hours and 30 minutes before the sciatic block. At 24 hours after the block, NCV, various reactive oxygen species, and Caspase-3 were evaluated to determine the extent of sciatic nerve injury. RESULTS: The DN rat model was successfully established. Compared with the DN control group, the postblock values of VF responses (DN-Con, 16.5 ± 1.3 g; DN + Bup, 19.1 ± 1.5 g, P < .001) and PWTL significantly increased (DN-Con, 13.3 ± 1.1 seconds; DN + Bup, 14.6 ± 1.1 seconds, P = .028); the NCV of sciatic nerve was significantly reduced (DN-Con, 38.8 ± 2.4 m/s, DN + Bup, 30.5 ± 2.0 m/s, P = .003), and sciatic nerve injury (as indicated by axonal area) was more severe in the bupivacaine-treated DN group (DN-Con, 11.6 ± 0.3 µm, DN + Bup, 7.5 ± 0.3 µm, P < .001). In addition, DPI treatment significantly improved nerve function (VF responses, 17.3 ± 1.3 g; PWTL, 13.4 ± 1.1 seconds; NCV, 35.6 ± 3.1 m/s) and mitigated loss of axonal area (9.6 ± 0.3 µm). Compared to the DN + Bup group (without DPI), the levels of lipid peroxides and hydroperoxides, as well as the protein expression of NOX2, NOX4, and Caspase-3, were significantly reduced in the DN + Bup + DPI group (P < .05). CONCLUSIONS: Subcutaneous injection of DPI appears to protect against the functional and neurohistological damage of bupivacaine-blocked sciatic nerves in a high-fat diet/streptozotocin-induced DN model.


Assuntos
Anestésicos Locais/administração & dosagem , Anestésicos Locais/efeitos adversos , Bupivacaína/efeitos adversos , Neuropatias Diabéticas/fisiopatologia , Estresse Oxidativo/efeitos dos fármacos , Nervo Isquiático/lesões , Animais , Eletrofisiologia , Inibidores Enzimáticos/uso terapêutico , Hiperalgesia/fisiopatologia , Peróxidos Lipídicos/química , Masculino , Bloqueio Nervoso , Oniocompostos/uso terapêutico , Traumatismos dos Nervos Periféricos/induzido quimicamente , Ratos , Ratos Sprague-Dawley , Neuropatia Ciática/fisiopatologia
4.
Ying Yong Sheng Tai Xue Bao ; 27(4): 1039-1045, 2016 Apr 22.
Artigo em Chinês | MEDLINE | ID: mdl-29732757

RESUMO

By performing a pot experiment, the study compared leaf and litter element concentration between the dry-red soil and vertisols, and analyzed the interactive effects of soil types and species on leaf nutrient concentration and nutrient resorption efficiency. The results showed that the soil type significantly affected the concentrations of N, P, Ca, Mg, Cu, Zn, Fe and N:P in leaves as well as the concentrations of N, P, Mn and N:P in leaf litters. Concentrations of N, Mn and N:P in leaves and litters derived from the dry-red soil were significantly higher than those from the vertisols. In contrast, concentrations of P, Ca, Mg, Fe, Cu and Zn in leaves and concentration of P in litters derived from the dry-red soil were significantly lower than those from the vertisols. Leaf N concentration was 34.8% higher, and leaf P concentration was 40.0% lower from the dry-red soil compared with those from the vertisols. N, P and K showed net resorption while the other elements showed accumulating patterns when leaf was senesced. Accumulation of Ca, Mg and Mn of senesced leaves was significantly higher on the dry-red soil than on the vertisols. Species identities only significantly affected leaf N concentration. Interactions of species and soil taxonomy significantly affected nutrient concentration neither in leaves nor in leaf litters, indicating that soil taxonomy influenced plant nutrient concentration in a similar way across multiple species. The influences of soil types on plant element concentration could have further effects on litter decomposition, plant-soil nutrient feedback and biogeochemical cycle in this dry and hot valley.


Assuntos
Folhas de Planta/química , Plantas/química , Solo/química , China , Meio Ambiente , Nitrogênio , Fósforo , Potássio
5.
Appl Opt ; 53(30): 7011-6, 2014 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-25402788

RESUMO

We achieve laser frequency stabilization by a simple technique based on sub-Doppler dichroic atomic vapor laser lock (DAVLL) in atomic cesium. The technique that combines saturated-absorption spectroscopy and Zeeman splitting of hyperfine structures allows us to obtain a modulation-free dispersion-like error signal for frequency stabilization. For the error signal, the dependence of peak-to-peak amplitude and the slope at the zero-crossing point on the magnetic field is studied by simulation and experiment. Based on the result, we obtain an available sub-Doppler DAVLL error signal with high sensitivity to the frequency drift by selecting an appropriate strength of the magnetic field. Ultimately, the fluctuation of the locked laser frequency is confined to below 0.5 MHz in a long term, exhibiting efficient suppression of frequency noise.

6.
Appl Opt ; 52(25): 6195-200, 2013 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-24085077

RESUMO

We present a simple, reliable, and nondestructive method for the measurement of vacuum pressure in a magneto-optical trap. The vacuum pressure is verified to be proportional to the collision rate constant between cold atoms and the background gas with a coefficient k, which can be calculated by means of the simple ideal gas law. The rate constant for loss due to collisions with all background gases can be derived from the total collision loss rate by a series of loading curves of cold atoms under different trapping laser intensities. The presented method is also applicable for other cold atomic systems and meets the miniaturization requirement of commercial applications.

7.
Ying Yong Sheng Tai Xue Bao ; 24(4): 956-60, 2013 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-23898651

RESUMO

Taking the arid-hot valley of Jinsha River, Southwest China as the object, a comparative study was made on the plant leaf N, P, and K concentrations and ratios as well as their relationships with species dominance in the restoration area and disturbed area, aimed to understand the effects of ecosystem restoration on the plant leaf stoichoimetric characteristics. Ecosystem restoration decreased the plant leaf N and P concentrations and P/K ratio significantly, but had lesser effects on the plant leaf K concentration. In restoration area, the plant leaf N, P, and K concentrations were averagely 10. 405, 0. 604, and 9. 619 g kg-1, being 16. 9% , 34. 9% , and 4. 7% lower than those in disturbed area, respectively. In restoration area, species dominance was significantly negatively correlated with plant leaf P concentration; while the species dominance in disturbed area had a significant negative correlation with plant leaf K concentration. Ecosystem restoration altered the slope and intercept of the scaling relationships among the plant leaf N, P, and K. No significant differences were observed in the leaf N, P, and K concentrations of the same plant species between restoration area and disturbed area, suggesting that the changes of plant leaf stoichiometric characteristics were mainly driven by the shift of species composition in the plant community.


Assuntos
Conservação dos Recursos Naturais , Ecossistema , Nitrogênio/análise , Fósforo/análise , Folhas de Planta/química , Potássio/análise , China , Recuperação e Remediação Ambiental , Temperatura Alta , Desenvolvimento Vegetal , Especificidade da Espécie
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