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1.
J Colloid Interface Sci ; 606(Pt 2): 1284-1298, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34492466

RESUMO

2D/2D heterojunction photocatalysts with excellent photocatalytic activity highlight considerable potential in water disinfection. Here, an oxidized Sb/g-C3N4 2D/2D nanosheets heterojunction (Sb-SbOx/CNS) was constructed based on a facile one-step liquid-phase exfoliation method using concentrated sulfuric acid. By doing so, bulk Sb and g-C3N4 were exfoliated simultaneously and then, intercalated each other. Compared with CNS and Sb-SbOx, the obtained Sb-SbOx/CNS demonstrated better photocatalytic disinfection activity towards Escherichia coli K-12 (E. coli K-12) under visible light irradiation. The 5% oxidized Sb/g-C3N4 2D/2D nanosheets heterojunction (5.0% Sb-SbOx/CNS) exhibited the best photocatalytic performance and admirable cycling stability, which was ascribed to the unique structure where the interfacial charge separation was strengthened by the strong coupling effect between Sb-SbOx and CNS. Meanwhile, the fundamental mechanism of photocatalytic disinfection was also proposed. The photogenerated ROS (reactive oxygen species) violently attacked the E. coli K-12 membrane, creating massive and irreparable holes on the cell membrane. The leakage of cations (K+, Na+, Ca2+ and Mg2+), adenosine triphosphate, total soluble sugar and protein accelerated the destruction of E. coli K-12. Trapping experiments suggested that the photocatalytic disinfection process against E. coli K-12 was dominated by h+ generated on 5.0% Sb-SbOx/CNS. This work offers a new promising way to modify the 2D/2D heterojunction featuring efficient photocatalytic disinfection performance.


Assuntos
Desinfecção , Escherichia coli K12 , Catálise , Escherichia coli , Luz
2.
Chemosphere ; 287(Pt 3): 132292, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34562711

RESUMO

The insight into the neglected reduction process accompanied by UV-based oxidation process may provide new ideas for the development of advanced oxidation and reduction technologies. In this study, aniline was comprehensively investigated as an unexpected indicator of hydrated electron (eaq-) under UV irradiation. Monochloroacetic acid (MCAA) was selected as the probe of eaq- and the balance of chloride ions indicated the reduction of MCAA. Further, laser flash photolysis experiments demonstrated the generation of eaq- in the UV/aniline process and the half-life period of formed eaq- was demonstrated to be 0.13 µs. The photolysis of aniline along with the decay of the excited state of aniline was responsible for the eaq- generation. Besides, the hydrogen atom (H) generated from the photolysis can subsequently reacted with OH- to generate eaq-. The photolysis pathways of aniline were proposed by the results of GC-MS. Aniline was abstracted of H in solution to the formation of aniline radical (PhNH) or form aminophenol in three different isomers (orto-, meta- and para-aminophenol). Moreover, UV/aniline showed a higher reducing capacity of MCAA compared with other organic electron donors and sustained a highly reducing ability in a wide pH. And the calculation results of quantum efficiency (Φ) showed that excessive aniline was not conducive to the elevation of Φ. This study introduced a novel pathway of eaq- generation during the photolysis of aniline and provided a new perspective for eaq--based advanced reduction processes.


Assuntos
Elétrons , Poluentes Químicos da Água , Compostos de Anilina , Oxirredução , Fotólise , Raios Ultravioleta , Poluentes Químicos da Água/análise
3.
Int J Endocrinol ; 2021: 7752526, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34721574

RESUMO

Semaphorin (SEMA) has an important role in nerve development, organ formation, immune response, angiogenesis, and tumor growth. SEMA can regulate the growth and branching of axons, the morphology of dendrites, and the migration of neurons. The loss-of-function in SEMA and its receptors PLXNs and NRP affect the migration of GnRH neurons, leading to idiopathic hypogonadotropic hypogonadism (IHH). As a member of the SEMA family, SEMA3A has an important role in axonal rejection, dendritic branching, synaptic formation, and neuronal migration. There are more and more SEMA3A variants identified in IHH patients. In this study, we identified a novel SEMA3A variant (c.1369A > G (p.T457A)) in a male nIHH patient. Functional studies indicated that the T457A SEMA3A variant led to the defect of FAK phosphorylation and GN11 cell migration, which strongly argued in favor of its pathogenic effect in the nIHH patient. Our findings substantiated that the 435-457 position of SEMA3A might be very important for the secretion of SEMA3A. Haploin-sufficiency of SEMA3A in humans was sufficient to cause the IHH phenotype. SEMA3A variants might have a role in modifying the IHH phenotype, according to the variants at different positions of SEMA3A. SEMAs and its receptors formed a complex network, and other members of the SEMA-signaling pathway might also be involved in the pathogenesis of IHH.

4.
Artigo em Inglês | MEDLINE | ID: mdl-34791274

RESUMO

BACKGROUND: The success rate of conventional Helicobacter pylori eradication therapy is declining, due to rising antibiotic resistance. OBJECTIVES: To determine the temporal effects of prior antibiotic exposure on eradication outcome. PATIENTS AND METHODS: This is a retrospective cohort study including all H. pylori-infected patients who received their first course of clarithromycin-containing triple therapy in 2003-18. Prior antibiotic exposures before H. pylori eradication therapy (up to 180 days, 1 year or 3 years) were retrieved. A logistic regression model was used to evaluate the association between different timings of previous antibiotic exposure, recent (within 30/60 days) or distant period, and the need for retreatment for H. pylori. RESULTS: A total of 120 787 H. pylori-infected patients were included. Prior exposure to any antibiotics within 180 days was associated with a higher risk of retreatment (OR 1.18, 95% CI 1.13-1.24) and the risk progressively increased with longer duration of antibiotic use. The results were consistent for prior exposure up to 1 year (OR 1.26, 95% CI 1.20-1.31) or 3 years (OR 1.30, 95% CI 1.25-1.35). However, when compared with those without prior antibiotic exposure, recent exposure (within 30 days) did not increase the risk of retreatment, which was consistent for analysis with prior antibiotic exposure up to 3 years. Notably, recent use of cephalosporins within 30/60 days and nitroimidazole within 30 days had significantly lower risks of retreatment. CONCLUSIONS: Any prior antibiotic exposure increased the risk of treatment failure of clarithromycin-containing triple therapy. Recent exposures to some classes of antibiotics may paradoxically increase treatment success.

5.
Multisens Res ; : 1-19, 2021 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-34638103

RESUMO

Reliable duration perception is an integral aspect of daily life that impacts everyday perception, motor coordination, and subjective passage of time. The Scalar Expectancy Theory (SET) is a common model that explains how an internal pacemaker, gated by an external stimulus-driven switch, accumulates pulses during sensory events and compares these accumulated pulses to a reference memory duration for subsequent duration estimation. Second-order mechanisms, such as multisensory integration (MSI) and attention, can influence this model and affect duration perception. For instance, diverting attention away from temporal features could delay the switch closure or temporarily open the accumulator, altering pulse accumulation and distorting duration perception. In crossmodal duration perception, auditory signals of unequal duration can induce perceptual compression and expansion of durations of visual stimuli, presumably via auditory influence on the visual clock. The current project aimed to investigate the role of temporal (stimulus alignment) and nontemporal (stimulus complexity) features on crossmodal, specifically auditory over visual, duration perception. While temporal alignment revealed a larger impact on the strength of crossmodal duration percepts compared to stimulus complexity, both features showcase auditory dominance in processing visual duration.

7.
Mol Genet Genomic Med ; 9(11): e1816, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34636164

RESUMO

BACKGROUND: Isolated hypogonadotropic hypogonadism (IHH) is a clinical syndrome described by failure of gonadal function secondary to defects on the synthesis, secretion, or action of the gonadotropin-releasing hormone (GnRH). The secreted glycoprotein SEMA3A binds its receptors NRP1 or NRP2 and PLXNA to participate in axonal projection, dendritic branching, synaptic formation, and neuronal migration. Deficiency in SEMA3A, NRP1, NRP2, and PLXNA1 have been related to abnormal GnRH neuron development in mice and IHH in humans. METHODS: The aim of this study was to examine the genotypic and phenotypic spectra of the NRP1, NRP2, and PLXNA1 genes in a large cohort of IHH probands from China. We screened NRP1, NRP2, and PLXNA1 variants in Chinese IHH patients by whole exome sequencing and pedigree analysis. RESULTS: We identified 10 heterozygous missense variants in PLXNA1, five heterozygous missense variants in NRP1, and two heterozygous missense variants in NRP2. NRP1 variants were found only in IHH patients with defective olfaction (i.e., Kallmann syndrome, KS). In addition, 85% (17/20) of patients harbored variants in other IHH-associated genes. CONCLUSION: Our study greatly enriched the genotypic and phenotypic spectra of PLXNA1, NRP1, and NRP2 in IHH. It may be conducive to the genetic counseling, diagnosis, and treatment of IHH with mutations in the PLXNA1, NRP1, and NRP2 genes. Furthermore, our results indicated that NRP1 were strongly linked to hearing loss.

8.
Front Aging Neurosci ; 13: 700787, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34630067

RESUMO

Impaired temporal perception of multisensory cues is a common phenomenon observed in older adults that can lead to unreliable percepts of the external world. For instance, the sound induced flash illusion (SIFI) can induce an illusory percept of a second flash by presenting a beep close in time to an initial flash-beep pair. Older adults that have enhanced susceptibility to a fall demonstrate significantly stronger illusion percepts during the SIFI task compared to those older adults without any history of falling. We hypothesize that a global inhibitory deficit may be driving the impairments across both postural stability and multisensory function in older adults with a fall history (FH). We investigated oscillatory activity and perceptual performance during the SIFI task, to understand how active sensory processing, measured by gamma (30-80 Hz) power, was regulated by alpha activity (8-13 Hz), oscillations that reflect inhibitory control. Compared to young adults (YA), the FH and non-faller (NF) groups demonstrated enhanced susceptibility to the SIFI. Further, the FH group had significantly greater illusion strength compared to the NF group. The FH group also showed significantly impaired performance relative to YA during congruent trials (2 flash-beep pairs resulting in veridical perception of 2 flashes). In illusion compared to non-illusion trials, the NF group demonstrated reduced alpha power (or diminished inhibitory control). Relative to YA and NF, the FH group showed reduced phase-amplitude coupling between alpha and gamma activity in non-illusion trials. This loss of inhibitory capacity over sensory processing in FH compared to NF suggests a more severe change than that consequent of natural aging.

9.
Neuropsychologia ; 163: 108069, 2021 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-34715119

RESUMO

Navigating the world relies on understanding progressive sequences of multisensory events across time. Early deaf (ED) individuals are more precise in visual detection of space and motion than their normal hearing (NH) counterparts. However, whether ED individuals show altered multisensory temporal processing abilities is less clear. According to the connectome model, brain development depends on experience, and therefore the lack of audition may affect how the brain responds to remaining senses and how they are functionally connected. We used a temporal order judgment (TOJ) task to examine multisensory (visuotactile) temporal processing in ED and NH groups. We quantified BOLD responses and functional connectivity (FC) in both groups. ED and NH groups performed similarly for the visuotactile TOJ task. Bilateral posterior superior temporal sulcus (pSTS) BOLD responses during the TOJ task were significantly larger in the ED group than in NH. Using anatomically defined pSTS seeds, our FC analysis revealed stronger somatomotor and weaker visual regional connections in the ED group than in NH during the TOJ task. These results suggest that a lack of auditory input might alter the balance of tactile and visual area FC with pSTS when a multisensory temporal task is involved.

10.
Animals (Basel) ; 11(9)2021 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-34573550

RESUMO

The ability of bamboo charcoal to reduce the negative effects of high dietary selenium (Se) concentrations was assessed by feeding juvenile blunt snout bream (Megalobrama amblycephala) one of five Se-rich diets (1.5 mg/kg Se; 36% protein, 8.7% lipid) containing graded levels (0-4 g/kg) of bamboo charcoal powder for eight weeks. There were four tanks (350 L) of fish (initial weight 16.0 ± 0.5 g) for each treatment, and the fish were fed to satiation four times each day. At the end of the feeding trial, all of the fish from each tank were weighed to calculate the growth performance. Blood samples were firstly obtained to collect plasma for the biochemical indexes determination. Liver tissues were then collected to determine the antioxidant enzyme activities and gene expression. Dorsal muscles were also collected to determine the nutrient composition. The results show that when the bamboo charcoal content in the Se-rich feed ranged between 0 and 3 g/kg, the weight growth rate (WGR) and specific growth rate (SGR) values increased with the higher dietary bamboo charcoal content, and the maximum WGR and SGR values were achieved when the bamboo charcoal content in the Se-rich feed was 2-3 g/kg (p < 0.05). The Se content in muscle tissues decreased significantly with the increased bamboo charcoal content (p < 0.05) in the Se-rich feed, which ranged from 0 to 4 g/kg. When the bamboo charcoal content in the Se-rich feed was 2-3 g/kg, the levels of glucose (GLU) and albumin (ALB) in plasma reached a maximum (p < 0.05), whereas the level of alkaline phosphatase (ALP) reached a minimum (p < 0.05). Additionally, the activities of catalase (CAT), total superoxide dismutase (T-SOD), total antioxidative capacity (T-AOC), and glutathione peroxidase (GSH-Px) were significantly enhanced (p < 0.05) when the bamboo charcoal content was 3 g/kg. In contrast, the malondialdehyde (MDA) level increased sharply when the bamboo charcoal content in the Se-rich feed was 1 g/kg, compared to the control group and the groups supplemented with 2-3 g/kg bamboo charcoal (p < 0.05). Regarding mRNA-level gene expression, the results show that dietary supplementation with 0 to 3 g/kg of bamboo charcoal increased the expression of keap1 and nrf2, whereas nfkb expression was inhibited (p < 0.05). The mRNA expression of the antioxidant enzymes cat, gpx, and mn-sod was consistently enhanced in the group fed with the 3 g/kg bamboo charcoal diet (p < 0.05). The expression of the pro-inflammatory cytokines tnfα and tgfß was inhibited in the groups supplemented with 2-3 g/kg bamboo charcoal, whereas the expression of anti-inflammatory cytokines (il10) increased in the bamboo charcoal supplementation groups compared to the control group (p < 0.05). Generally, supplementation with 2-3 g/kg of bamboo charcoal in Se-rich feed improved the growth performance, physiological status, and antioxidant enzyme activities of blunt snout bream. Moreover, bamboo charcoal supplementation in Se-rich diets stimulated the antioxidant system and inhibited the inflammatory response by activating Nrf2-Keap1 and suppressing NF-κB.

11.
ACS Appl Mater Interfaces ; 13(45): 53425-53438, 2021 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-34482690

RESUMO

Designing flame-retardant polymers with high performance is a long-standing challenge, partly because of the time-consuming traditional approaches based on experiential intuition and trial-and-error screenings. Inspired by the effective new paradigm of data-driven material discovery, we used machine learning to analyze experimental data to accelerate the development of new flame-retardant polymers. To explore the relationship between limit oxygen index (LOI) and components, we prepared 20 composites and then trained a simple equation for the LOI using the method sure independence screening and sparsifying operator (SISSO). The data analysis allows us for a better understanding of the flame-retardant mechanism and components, and the equation has good accuracy in guiding the design of composites with high flame-retardant performance. Meanwhile, the increasing structural design of flame retardants is crucial to flame-retardant polymer composites. We proposed a structure of nano graphene oxide (GO) wrapped micro zinc hydroxystannate (ZHS) in a simple but effective way as a novel flame-retardant agent to enhance the flame retardancy and mechanical properties of polypropylene (PP) composites. The GO sheets were like "light yarns" wrapped onto the ZHS via hydrogen bonding in an ethanol solution. The selected samples were analyzed to confirm the predictive LOI model. The resultant composites with the substitution of intumescent flame retardant (IFR) by 1.0, 2.0, and 4.0 wt % ZHS@GO conferred better flame retardancy compared with PP composite containing only IFR, reflected by the efficient increase of LOI value and V0 rating of UL-94 vertical tests. The analysis principles and facile fabrication strategies proposed in this work could be important for developing highly flame retardant composites.

12.
Aliment Pharmacol Ther ; 54(9): 1162-1169, 2021 11.
Artigo em Inglês | MEDLINE | ID: mdl-34528716

RESUMO

BACKGROUND: The long-term effects of H. pylori eradication in preventing upper GI bleeding (UGIB) remains unknown. AIM: To determine the long-term risks of UGIB after H. pylori eradication METHODS: We included all patients who had received clarithromycin-containing triple therapy for the treatment of H. pyliori infection between 2003 and 2012, without subsequent need for re-treatment. We included a propensity score (PS)-matched endoscopy cohort of H. pylori-negative patients as controls. The primary endpoint was the risk of subsequent UGIB. A multivariable Cox model was used to compute the hazard ratio (HR) of UGIB. RESULTS: We included 62 738 H. pylori-eradicated and 62 738 PS-matched H. pylori-negative patients, with a median follow-up of 8.1 years (IQR 5.5-10.6). The incidence of UGIB was 20.8 (95% CI 19.5-22.1) and 13.6 (95% CI 12.7-14.7) per 10 000 person-years in H. pylori-eradicated and H. pylori-negative patients, respectively. Compared to controls, H. pylori-eradicated patients had a significantly higher risk of UGIB (HR: 1.65, 95% CI 1.49-1.83). The risk of UGIB in H. pylori-eradicated patients increased after the first 2 years of follow up (HR: 2.18, 95% CI 1.91-2.49). Age-stratified analysis showed that patients >45 years had higher UGIB risk, even after eradication. CONCLUSIONS: Despite H. pylori eradication, the long-term risk of UGIB was still higher than in H. pylori-negative control subjects. The protective effects of eradication therapy in preventing UGIB appeared to be limited to younger patients, and to within the first 2 years after eradication.


Assuntos
Infecções por Helicobacter , Helicobacter pylori , Antibacterianos/efeitos adversos , Claritromicina/efeitos adversos , Estudos de Coortes , Quimioterapia Combinada , Hemorragia Gastrointestinal/induzido quimicamente , Hemorragia Gastrointestinal/epidemiologia , Infecções por Helicobacter/complicações , Infecções por Helicobacter/tratamento farmacológico , Infecções por Helicobacter/epidemiologia , Humanos , Pessoa de Meia-Idade
13.
Neuroscience ; 472: 138-156, 2021 09 15.
Artigo em Inglês | MEDLINE | ID: mdl-34333061

RESUMO

Establishing consistent relationships between neural activity and behavior is a challenge in human cognitive neuroscience research. We addressed this issue using variable time constraints in an oddball frequency-sweep design for visual discrimination of complex images (face exemplars). Sixteen participants viewed sequences of ascending presentation durations, from 25 to 333 ms (40-3 Hz stimulation rate) while their electroencephalogram (EEG) was recorded. Throughout each sequence, the same unfamiliar face picture was repeated with variable size and luminance changes while different unfamiliar facial identities appeared every 1 s (1 Hz). A neural face individuation response, tagged at 1 Hz and its unique harmonics, emerged over the occipito-temporal cortex at 50 ms stimulus duration (25-100 ms across individuals), with an optimal response reached at 170 ms stimulus duration. In a subsequent experiment, identity changes appeared non-periodically within fixed-frequency sequences while the same participants performed an explicit face individuation task. The behavioral face individuation response also emerged at 50 ms presentation time, and behavioral accuracy correlated with individual participants' neural response amplitude in a weighted middle stimulus duration range (50-125 ms). Moreover, the latency of the neural response peaking between 180 and 200 ms correlated strongly with individuals' behavioral accuracy in this middle duration range, as measured independently. These observations point to the minimal (50 ms) and optimal (170 ms) stimulus durations for human face individuation and provide novel evidence that inter-individual differences in the magnitude and latency of early, high-level neural responses are predictive of behavioral differences in performance at this function.


Assuntos
Reconhecimento Facial , Discriminação Psicológica , Eletroencefalografia , Face , Humanos , Reconhecimento Visual de Modelos , Estimulação Luminosa
14.
ACS Appl Mater Interfaces ; 13(34): 40618-40628, 2021 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-34416111

RESUMO

Nitrogen reduction reaction (NRR), as a green and sustainable technology, is far from a practical application due to the lack of efficient electrocatalysts. In this work, we found that antimonene, a group-VA elemental two-dimensional (2D) material, is attractive as an electrocatalyst for NRR. The antimonene here is acquired through chemical exfoliation of antimony (Sb) using H2SO4 for the first time, which simultaneously achieved efficient large-sized exfoliation and created a high density of active edge sites. Moreover, the concentration of defects shows a gradual increasing tendency as the treatment time extends. The obtained antimonene exhibited favorable average ammonia (NH3) yield and Faradaic efficiency as high as 2.08 µg h-1 cm-2 and 14.25% at -0.7 V versus RHE, respectively. Density functional theory calculations prove that the sufficient exposure of edge defects is favorable for reducing the reaction barrier and strengthening the interaction between antimonene and the intermediates of NRR, thus increasing the selectivity and yield rate of NH3. The chemical exfoliation of Sb reported here offers an alternative avenue to engineer the surface structures of group-VA elemental-based catalysts. Investigation of NRR using 2D antimonene can further provide deep insight into the mechanism and principle of NRR over group-VA elemental nanosheets.

15.
Front Physiol ; 12: 706579, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34421644

RESUMO

Mantis shrimp Oratosquilla oratoria is an economically critical aquatic species along the coast of China but strongly accumulates marine pollutant cadmium (Cd) in its digestive system. It is necessary to characterize the toxicity of Cd in the digestive system of mantis shrimp. The metabolic process is an essential target of Cd toxicity response. In this work, we used ultra-performance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC-TOF-MS) for untargeted metabolomics to characterize the metabolic changes in the digestive system of O. oratoria, exposed to 0.05 mg/L for 96 h. The aim of this study was to further investigate the effect of O. oratoria on Cd response to toxicity and develop biomarkers. Metabolomics analysis showed the alteration of metabolism in the digestive system of mantis shrimp under Cd stress. A total of 91 metabolites were differentially expressed and their main functions were classified into amino acids, phospholipids, and fatty acid esters. The enrichment results of differential metabolite functional pathways showed that biological processes such as amino acid metabolism, transmembrane transport, energy metabolism, and signal transduction are significantly affected. Based on the above results, the Cd-induced oxidative stress and energy metabolism disorders were characterized by the differential expression of amino acids and ADP in mantis shrimp, while the interference of transmembrane transport and signal transduction was due to the differential expression of phospholipids. Overall, this work initially discussed the toxicological response of Cd stress to O. oratoria from the metabolic level and provided new insights into the mechanism.

16.
Mol Immunol ; 139: 42-49, 2021 11.
Artigo em Inglês | MEDLINE | ID: mdl-34454184

RESUMO

Accumulating lines of evidence have revealed the involvement of long non-coding RNAs (lncRNAs) in the control and elimination of invading Mycobacterium tuberculosis (Mtb) by macrophage. In this study, we sought to elucidate the role of MIAT on autophagy and apoptosis of Mtb-infected macrophage and to reveal the molecular mechanism. We observed that the expression of MIAT was heightened while miR-665 level was declined in THP-1 cells with Bacillus Calmette-Guerin (BCG) infection in a time-dependent manner. Functionally, disruption of MIAT effectively facilitated cell viability and restricted apoptosis ability concomitant with the downregulation of Bax and cleaved caspase-3 along with an accumulation of Bcl-2 in BCG-infected THP-1 cells. Concurrently, the interference of MIAT dramatically disinhibited macrophage autophagy as characterized by diminution of autophagy related markers LC3-II and Beclin-1 as well as increment of p62 in THP-1 cells following BCG infection. Concordantly, depletion of MIAT was found to noticeably aggrandize Mtb survival. Importantly, MIAT served as a ceRNA for sponging miR-665 and negatively regulated its expression. ULK1 was identified as an authentic target of miR-665 and modulated by MIAT. Mechanistically, the functional role of MIAT depletion in macrophage apoptosis and autophagy were tremendously abrogated by the depression of miR-665 and enrichment of ULK1. Overall, the preceding observations clearly illuminated that MIAT was elevated in human macrophage response to BCG infection, and functioned as a negative regulator in autophagy and antimicrobial effects by manipulating miR-665/ULK1 axis during Mtb infection, which may provide a promising target for developing an anti-bacterial against TB.


Assuntos
Proteína Homóloga à Proteína-1 Relacionada à Autofagia/imunologia , Peptídeos e Proteínas de Sinalização Intracelular/imunologia , Macrófagos/imunologia , MicroRNAs/imunologia , RNA Longo não Codificante/imunologia , Tuberculose/imunologia , Apoptose/imunologia , Autofagia/imunologia , Proteína Homóloga à Proteína-1 Relacionada à Autofagia/metabolismo , Regulação da Expressão Gênica/imunologia , Humanos , Peptídeos e Proteínas de Sinalização Intracelular/metabolismo , Macrófagos/metabolismo , Macrófagos/microbiologia , MicroRNAs/metabolismo , Mycobacterium tuberculosis , RNA Longo não Codificante/metabolismo , Transdução de Sinais/imunologia , Células THP-1
17.
Am J Transl Res ; 13(6): 6211-6220, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34306360

RESUMO

OBJECTIVE: To explore the efficacy of the acrid-release and bitter-downbearing therapy and Banxia Xiexin Decoction (BXD) in treating gastric cancer (GC). METHODS: BXD was decocted, and serum containing medicine was prepared from rats. The SNU-16 cells were cultured with different concentrations of BXD serum (25, 50, 100 µL/mL). Then, those were treated with BXD and Wnt/ß-catenin pathway activator (LiCl) and divided into three groups: Control group, BXD group and BXD+LiCl group. Activation of the Wnt/ß-catenin pathway was detected by immunofluorescence staining, qRT-PCR, and western blot. Cell activity, clone formation, invasion, metastasis and apoptosis in each group were examined by MTT, clone formation test, Transwell and flow cytometry. The oxidative stress measures in cells of each group were tested by an oxidative stress kit. RESULTS: With increasing BXD concentration, the clonogenic ability of cells was inhibited. BXD can inhibit cell activity, clone formation, invasion and metastasis, promote oxidative stress, and induce apoptosis. It can also inhibit the activation of Wnt/ß-catenin signaling pathway. A Wnt/ß-catenin signaling pathway activator could partially inhibit the action of BXD. CONCLUSION: BXD participates in GC treatment by inhibiting Wnt/ß-catenin signaling pathway, thus inhibiting GC cell activity and clone formation, promoting oxidative stress, and inducing apoptosis.

18.
J Hazard Mater ; 418: 126448, 2021 09 15.
Artigo em Inglês | MEDLINE | ID: mdl-34315633

RESUMO

Denitrification is an effective strategy to control eutrophication caused by excessive nitrate in water. However, the comparatively low efficiency of nitrate removal and N2 selectivity remains a challenge in the denitrification process. Herein, this study proposed a novel photochemical denitrification process by introducing hydrated electron (eaq-) to reduce nitrate in UV/sulfite system. The results indicated that the optimized UV/sulfite system could effectively reduce nitrate to N2 with a nearly 100% denitrification efficiency in 90 min. eaq- was identified as the mainly reactive species to achieve rapid removal of nitrate and nitrite, and the result was verified by quenching and laser flash photolysis tests. Benefiting from the high dispersion of eaq- in water and the rapid reaction rate between eaq- and the target, the generated N2O is susceptible to be reduced, leading to a high selectivity of N2 that was confirmed by 15N-isotopic. Besides, thermodynamic results based on the density functional theory (DFT) calculations suggested that the photochemical denitrification process was exothermic process and tend to transform to N2. Significantly, UV/sulfite system applied in the nickel-plating wastewater showed high denitrification efficiency, demonstrating that the novel photochemical denitrification process is promising for practical wastewater treatment.


Assuntos
Desnitrificação , Nitratos , Nitritos , Nitrogênio , Sulfitos , Águas Residuárias
19.
Vision Res ; 187: 129-136, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34252728

RESUMO

Normal aging results in pronounced optical and neural changes in the visual system. Processes of adaptation are thought to help compensate for many of these changes in order to maintain perceptual constancy, but it is uncertain how stable adaptation itself remains with aging. We compared the dynamics of adaptation in young (aged 19-24 years) and older (aged 66-74) adults. Contrast thresholds for Gabor patterns were tracked during and after 300 s adaptation to vertical and horizontal Gabor patches. The time course of contrast adaptation and asymptotic adaptation magnitude were similar between older and young adults when normalized for their respective baseline thresholds. Older adults showed stronger transfer of adaptation to the orthogonal orientation and there was an asymmetry between the transfer of adaptation between the horizontal and vertical orientations for both groups. These results suggest age-related changes in orientation tuning while the processes of cortical contrast adaptation remain largely intact with aging.

20.
Artigo em Inglês | MEDLINE | ID: mdl-34264496

RESUMO

As the global ecosystem has been severely disturbed by an increasing number of human activities at different scales, remote sensing technology, as an effective quantitative measure of environmental quality, has been widely used. The remote sensing ecological index (RSEI) is one of the most popular and comprehensive ecological quality assessment indices based on the remote sensing data. However, the RSEI model exhibits that the ecological environment under natural conditions is not limited by the spatial scales. In addition, the model has major shortcomings in index selection and eigenvector, which greatly limit the application of RSEI. In this paper, the RSEI model is improved and a remote sensing ecological index optimized by the regional scale (RO-RSEI) is proposed. The result of the study, conducted in Shuangyang District, Changchun City, Jilin Province, shows that the RO-RSEI model has regional ecological significance after the introduction of the scale theory of landscape ecology; the index is preferred to solve problems like the RSEI model applied mechanization and baseless index selection. Meanwhile, due to the optimization of the eigenvector contribution of the optimal index, it solves the problems like non-unique model calculation result caused by principal component analysis or even antipodal calculation result. Compared with the RSEI model, the monitoring result of RO-RSEI model can better reflect the regional ecological changes. The improved model offers the possibility of monitoring ecological environment quality with remote sensing big data and provides a scientific basis for future scholars' batch computing.

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