Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 17 de 17
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
BMC Plant Biol ; 20(1): 76, 2020 Feb 14.
Artigo em Inglês | MEDLINE | ID: mdl-32059642

RESUMO

BACKGROUND: Rice ratooning has traditionally been an important component of the rice cropping system in China. However, compared with the rice of the first harvest, few studies on factors effecting ratoon rice yield have been conducted. Because ratoon rice is a one-season rice cultivated using axillary buds that germinate on rice stakes and generate panicles after the first crop's harvest, its production is mainly affected by the growth of axillary buds. The objectives of this study were to evaluate the sprouting mechanism of axillary buds to improve the ratoon rice yield. RESULTS: First, we observed the differentiation and growth dynamics of axillary buds at different nodes of Shanyou 63, and found that they differentiated from bottom to top before the heading of the mother stem, and that they developed very slowly. After heading they differentiated from top to bottom, and the ones on the top, especially the top 2nd node, developed much faster than those at the other nodes. The average length and dry weight of the axillary buds were significantly greater than those at other nodes by the yellow ripe stage, and they differentiated into pistils and stamens by 6 d after the yellow ripe stage. The morphology of vegetative organs from regenerated tillers of Shanyou 63 also suggested the superior growth of the upper buds, which was regulated by hormones, in ratoon rice. Furthermore, a comprehensive proteome map of the rice axillary buds at the top 2nd node before and after the yellow ripe stage was established, and some proteins involved in steroid biosynthesis were significantly increased. Of these, four took part in brassinosteroid (BR) biosynthesis. Thus, BR signaling may play a role in the germination of axillary buds of ratoon rice. CONCLUSIONS: The data provide insights into the molecular mechanisms underlying BR signaling, and may allow researchers to explore further the biological functions of endogenous BRs in the germination of axillary buds of ratoon rice.

2.
Biomicrofluidics ; 13(6): 064108, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31737157

RESUMO

This paper describes a highly controllable method to generate hollow calcium alginate microfibers using a double co-axial flow microdevice. The microdevice was fabricated by concentric assembly of two modules; each module consisted of a shortened cone-pulled glass capillary embedded in a polymethylmethacrylate fluidic block. Using this microdevice, cylindrical hollow calcium alginate microfibers with either straight or helical inner walls were stably and continuously generated. The radii of the hollow microfibers were not arbitrary, and in fact, the ratio of the outer to inner diameter was inversely correlated with the combination of core flow rate and the first sheath flow rate. The relationships between the geometrical features of the helix and the flow rates were also analyzed. The helical pitch and the spiral radius of the helical hollow microfibers were strongly influenced by the second sheath flow rate. Finally, guidelines for generating highly controllable straight and helical hollow microfibers and fabricating a seamless flow connector using this microfluidic device are suggested.

3.
Anal Biochem ; 549: 91-95, 2018 05 15.
Artigo em Inglês | MEDLINE | ID: mdl-29548926

RESUMO

Aptamers are promising alternatives to antibodies and can be used as high affinity agents for the cancer detection and the targeted drug transportation. In this manuscript, we highlight the advantages of aptamers, such as high affinities, specificity and excellent chemical stabilities, which are likely to benefit for the diagnosis of cancer in its early stages and then achieve molecular-level treatment. Also, we discuss the challenges and problems in the current application of aptamers.


Assuntos
Antineoplásicos , Aptâmeros de Nucleotídeos , Sistemas de Liberação de Medicamentos/métodos , Neoplasias/tratamento farmacológico , Animais , Antineoplásicos/química , Antineoplásicos/uso terapêutico , Aptâmeros de Nucleotídeos/química , Aptâmeros de Nucleotídeos/uso terapêutico , Humanos , Neoplasias/metabolismo , Neoplasias/patologia
4.
Exp Ther Med ; 14(6): 5258-5266, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29285051

RESUMO

Previous studies have demonstrated that inflammatory cytokines are associated with matrix metalloproteinases (MMPs) and/or lysyl oxidases (LOXs) produced by anterior cruciate ligament (ACL) fibroblasts, which may contribute to the poor healing ability of the ACL. To evaluate whether the synovium also participates in ACL healing, the inflammatory microenvironment of the knee joint cavity was mimicked following ACL injury, and the combined effects of tumor necrosis factor-α (TNF-α) and interleukin-1ß (IL-1ß) on the expression of MMPs and LOXs in synovial fibroblasts were studied. Cell viability was evaluated using trypan blue staining in the presence of TNF-α and IL-1ß, and the expression of LOXs and MMPs was measured by reverse transcription-quantitative polymerase chain reaction. MMP-2 activity was also measured by zymography. The results indicated that the combined effects of TNF-α and IL-1ß inhibited LOX expression, while promoting MMP-1, -2 and -3 expression and MMP-2 activity in synovial fibroblasts. These changes may impede healing by altering the balance between the degradative and biosynthetic arms of the ligament tissue remodeling process. Collectively, the present results suggest that the poor healing ability of cruciate ligaments may be due to the sensitivity of the synovium to inflammatory factors. Therefore, the synovium potentially serves a key regulatory role in the joint cavity microenvironment and in the healing process of the ACL, and thus should be considered as a therapeutic target to aid in the treatment of patients with ACL trauma.

5.
Biofabrication ; 9(2): 025030, 2017 Jun 07.
Artigo em Inglês | MEDLINE | ID: mdl-28485303

RESUMO

Fabrication of small diameter vascular grafts (SDVGs) with appropriate responses for clinical application is still challenging. In the present work, the production and characterization of solid alginate based microfibers as potential SDVG candidates through the method of microfluidics were considered original. A simple glass microfluidic device with a 'L-shape' cylindrical-flow channel in the microfluidic platform was developed. The gelation of microfibers occurred when the alginate solution and a CaCl2 solution were introduced as a core flow and as a sheath flow, respectively. The diameters of the microfibers could be controlled by varying the flow rates and the glass capillary tubes diameters at their tips. The generated microfibers had somewhat rough and porous surfaces, their suture retention strengths were comparable to the strength of other tissue engineered grafts. The encapsulated mesenchymal stem cells proliferated well in the microfibers, and showed a stable endothelialization under the angiogenesis effects of vascular endothelial growth factor and fibroblastic growth factor. The in vivo implant into the mice abdomens indicated that cell composite microfibers caused a mild host reaction. These encouraging results suggest great promise of the application of microfluidics as a future alternative in SDVGs engineering.


Assuntos
Alginatos , Materiais Biocompatíveis , Prótese Vascular , Técnicas Analíticas Microfluídicas , Alginatos/química , Alginatos/metabolismo , Animais , Materiais Biocompatíveis/química , Materiais Biocompatíveis/metabolismo , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Desenho de Equipamento , Feminino , Fatores de Crescimento de Fibroblastos , Ácido Glucurônico/química , Ácido Glucurônico/metabolismo , Ácidos Hexurônicos/química , Ácidos Hexurônicos/metabolismo , Masculino , Células-Tronco Mesenquimais/citologia , Células-Tronco Mesenquimais/efeitos dos fármacos , Camundongos , Técnicas Analíticas Microfluídicas/instrumentação , Técnicas Analíticas Microfluídicas/métodos , Fator A de Crescimento do Endotélio Vascular/farmacocinética , Fator A de Crescimento do Endotélio Vascular/farmacologia
6.
Genetics ; 205(2): 993-1002, 2017 02.
Artigo em Inglês | MEDLINE | ID: mdl-27986805

RESUMO

Grain number is an important factor in determining grain production of rice (Oryza sativa L.). The molecular genetic basis for grain number is complex. Discovering new genes involved in regulating rice grain number increases our knowledge regarding its molecular mechanisms and aids breeding programs. Here, we identified GRAINS NUMBER 2 (GN2), a novel gene that is responsible for rice grain number, from "Yuanjiang" common wild rice (O. rufipogon Griff.). Transgenic plants overexpressing GN2 showed less grain number, reduced plant height, and later heading date than control plants. Interestingly, GN2 arose through the insertion of a 1094-bp sequence from LOC_Os02g45150 into the third exon of LOC_Os02g56630, and the inserted sequence recruited its nearby sequence to generate the chimeric GN2 The gene structure and expression pattern of GN2 were distinct from those of LOC_Os02g45150 and LOC_Os02g56630 Sequence analysis showed that GN2 may be generated in the natural population of Yuanjiang common wild rice. In this study, we identified a novel functional chimeric gene and also provided information regarding the molecular mechanisms regulating rice grain number.


Assuntos
Genes de Plantas , Oryza/genética , Plantas Geneticamente Modificadas , Sementes/genética , Mutagênese Insercional , Oryza/crescimento & desenvolvimento , Sementes/crescimento & desenvolvimento
7.
Micromachines (Basel) ; 8(10)2017 Sep 21.
Artigo em Inglês | MEDLINE | ID: mdl-30400477

RESUMO

We provide a facile and low-cost method (F-L) to fabricate a two-dimensional positive master using a milling technique for polydimethylsiloxane (PDMS)-based microchannel molding. This method comprises the following steps: (1) a positive microscale master of the geometry is milled on to an acrylic block; (2) pre-cured PDMS is used to mold the microscale positive master; (3) the PDMS plate is peeled off from the master and punctured with a blunt needle; and (4) the PDMS plate is O2 plasma bonded to a glass slide. Using this technique, we can fabricate microchannels with very simple protocols quickly and inexpensively. This method also avoids breakage of the end mill (ϕ = 0.4 mm) of the computerized numerical control (CNC) system when fabricating the narrow channels (width < 50 µm). The prominent surface roughness of the milled bottom-layer could be overcomed by pre-cured PDMS with size trade-off in design. Finally, emulsion formation successfully demonstrates the validity of the proposed fabrication protocol. This work represents an important step toward the use of a milling technique for PDMS-based microfabrication.

8.
Int Orthop ; 39(1): 183-91, 2015 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-25416119

RESUMO

PURPOSE: The adult human posterior cruciate ligament (PCL) has poor functional healing response. The synovial tissue, which surrounds the PCL ligament, might be the major regulator of the microenvironment in the joint cavity after PCL injury, thus affecting the healing process. Here we establish a novel co-culture system for PCL fibroblasts and synovial cells (SC) in vitro to explore the direct influence of paracrine on PCL cells by characterizing the different expressions of the lysyl oxidase family (LOXs) and matrix metalloproteinases (MMP-1, 2, 3), which respectively facilitate extracellular matrix (ECM) repair and degradation. METHODS: Total RNA was harvested, reverse transcribed and assessed by semi-quantitative PCR and real-time PCR for the expression of LOXs and MMP-1, 2, 3 messenger RNAs. MMP-2 activity was assayed from the collected culture media samples by using zymography. RESULTS: We found co-culture could promote gene expressions of the LOXs and MMP-1, 2, 3 in normal PCL fibroblasts. But in injured PCL, we found that matrix crosstalk induced an increase of the MMP-1, 2, 3 expressions and a down-regulation of the LOXs. CONCLUSION: Based on these results, the crosstalk between PCL and SC strongly modified homeostatic balance of ECM and appeared to have a significant impact on PCL wound healing; decreased expression of cross-linking enzymes (LOXs) and increased expression of ECM-degrading proteinases (MMP-1, 2, 3) might be of great contribution to poor healing ability of PCL ligament.


Assuntos
Fibroblastos/metabolismo , Metaloproteinases da Matriz/metabolismo , Ligamento Cruzado Posterior/citologia , Proteína-Lisina 6-Oxidase/metabolismo , Membrana Sinovial/metabolismo , Adulto , Células Cultivadas , Técnicas de Cocultura , Matriz Extracelular/metabolismo , Fibroblastos/citologia , Expressão Gênica , Humanos , Ligamento Cruzado Posterior/metabolismo , Reação em Cadeia da Polimerase em Tempo Real , Membrana Sinovial/citologia , Cicatrização/fisiologia
9.
Knee ; 21(1): 47-53, 2014 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-23490076

RESUMO

BACKGROUND: The lysyl oxidase (LOX) family has the capacity to catalyze the cross-linking of collagen and elastin, implicating its important fundamental role in injury healing. Tumor necrosis factor alpha (TNF-α) is considered to be an important chemical mediator in the acute inflammatory phase of the ligament injury. The role of the lysyl oxidase family induced by TNF-α in the knee ligaments' wound healing process is poorly understood. Our purpose was to determine the different expressions of the LOXs in poorly self-healing anterior cruciate ligament (ACL) and well functionally self-healing medial collateral ligament (MCL) induced by TNF-α. METHODS: Semi-quantitative PCR, quantitative real-time PCR and western blot were performed for original research. RESULTS: The results showed that all LOX family members were expressed at higher levels in MCL than those in ACL fibroblasts; the significant differences existed in the down-regulations of the LOXs induced by TNF-α; and the TNF-α-mediated down-regulations of the LOXs were more prominent in ACL than those in MCL fibroblasts. 1-20 ng/ml TNF-α down-regulated mRNA levels in ACL and MCL fibroblasts by up to 76% and 58% in LOX; 90% and 45% in LOXL-1; 97.5% and 90% in LOXL-2; 89% and 68% in LOXL-3; 52% and 25% in LOXL-4, respectively. Protein assay also showed LOXs had lower expressions in ACL than those in MCL. CLINICAL RELEVANCE: Based on these results, the differential expressions of the LOXs might help to explain the intrinsic differences between the poorly self-healing ACL and well functionally self-healing MCL.


Assuntos
Ligamento Cruzado Anterior/citologia , Regulação para Baixo , Fibroblastos/metabolismo , Ligamento Colateral Médio do Joelho/citologia , Proteína-Lisina 6-Oxidase/metabolismo , Fator de Necrose Tumoral alfa/administração & dosagem , Adulto , Análise de Variância , Western Blotting , Sobrevivência Celular , Células Cultivadas , Relação Dose-Resposta a Droga , Eletroforese em Gel de Ágar , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Proteína-Lisina 6-Oxidase/genética , RNA Mensageiro/metabolismo
10.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi ; 30(4): 777-81, 2013 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-24059055

RESUMO

The progress of research on the the anterior cruciate ligament (ACL) wound healing demonstrates that the synovial tissue in the knee joint plays a very important role in the healing process of injured ACL. Therefore, the molecular response mechanisms of lysyl oxidase (LOX) and matrix metalloproteina (MMP) in normal/injured ACL fibroblast cells could be considered to perform the major analysis function of injured ACL healing mechanism. The mRNA expressions of LOXs and MMPs and the activity expressions of MMP-2 in ACL fibroblasts co-cultured with synovial cells were analyzed by quantitative real-time PCR and zymography. The results showed that co-culture could regulate the mRNA expressions of LOXs and MMPs in the ACL fibroblasts cells. These results suggest that the differential expressions of LOXs and MMP-1, 2, 3 in co-cultured ACL indicate that interaction crosstalk do exist between ACL cells and synovial cells and provide a theoretical basis for subsequent exploration of the mechanisms and treatment of ACL injury and repair.


Assuntos
Ligamento Cruzado Anterior/citologia , Fibroblastos/metabolismo , Metaloproteinases da Matriz/metabolismo , Proteína-Lisina 6-Oxidase/metabolismo , Membrana Sinovial/citologia , Lesões do Ligamento Cruzado Anterior , Técnicas de Cocultura , Fibroblastos/citologia , Humanos , Traumatismos do Joelho/fisiopatologia , Articulação do Joelho/citologia , Metaloproteinases da Matriz/genética , Proteína-Lisina 6-Oxidase/genética , Cicatrização/fisiologia
11.
Injury ; 44(7): 893-900, 2013 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-23010071

RESUMO

Lysyl oxidase (LOX) family has the capacity to catalyse the cross-linking of collagen and elastin, implicating its important fundamental roles in tissue development and injury healing. However, the variations in expression of the LOX family in the normal and injured anterior cruciate ligament (ACL) are not fully known. To better understand the role of LOX family in the self-healing inability mechanism of injured ACL, this study is to measure the LOX family's differential expressions in ACL and medial collateral ligament (MCL) fibroblasts after mechanical injury induced by using an equi-biaxial stretching chamber. The cells received various degrees of mechanical stretch 0% (resting state), 6% (physiological state) and 12% (injurious state), respectively. The gene profile and protein expressions were analysed by semi-quantitative PCR, quantitative real-time PCR and Western blotting. At physiological state, gene expression showed LOX in ACL was 2.6-5.2 folds higher than that in MCL in all culture time periods, LOXL-4 1.2-3.6 folds, but LOXL-3 in MCL showed 1.1-4.8 folds higher than that in ACL. In injurious state, MCL gene expressions were 2.8-29.6 folds higher than ACL in LOX, LOXL-2, LOXL-3 and LOXL-4 at 2, 6 and 12h periods. These differential expression profiles of the LOX family in the two ligament tissues were further used to explain the intrinsic differences between ACL and MCL, and why injured ACL could not be amenable to repair itself, whereas MCL could.


Assuntos
Fibroblastos/citologia , Ligamentos/lesões , Proteína-Lisina 6-Oxidase/biossíntese , Cicatrização/fisiologia , Adulto , Ligamento Cruzado Anterior/citologia , Ligamento Cruzado Anterior/metabolismo , Lesões do Ligamento Cruzado Anterior , Feminino , Fibroblastos/metabolismo , Humanos , Masculino , Ligamento Colateral Médio do Joelho/citologia , Ligamento Colateral Médio do Joelho/lesões , Ligamento Colateral Médio do Joelho/metabolismo , Pessoa de Meia-Idade , Proteína-Lisina 6-Oxidase/metabolismo
12.
J Genet Genomics ; 39(10): 551-60, 2012 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-23089365

RESUMO

Tiller angle is a key feature of the architecture of cultivated rice (Oryza sativa), since it determines planting density and influences rice yield. Our previous work identified Tiller Angle Control 1 (TAC1) as a major quantitative trait locus that controls rice tiller angle. To further clarify the evolutionary characterization of the TAC1 gene, we compared a TAC1-containing 3164-bp genomic region among 113 cultivated varieties and 48 accessions of wild rice, including 43 accessions of O. rufipogon and five accessions of O. nivara. Only one single nucleotide polymorphism (SNP), a synonymous substitution, was detected in TAC1 coding regions of the cultivated rice varieties, whereas one synonymous and one nonsynonymous SNP were detected among the TAC1 coding regions of wild rice accessions. These data indicate that little natural mutation and modification in the TAC1 coding region occurred within the cultivated rice and its progenitor during evolution. Nucleotide diversities in the TAC1 gene regions of O. sativa and O. rufipogon of 0.00116 and 0.00112, respectively, further indicate that TAC1 has been highly conserved during the course of rice domestication. A functional nucleotide polymorphism (FNP) of TAC1 was only found in the japonica rice group. A neutrality test revealed strong selection, especially in the 3'-flanking region of the TAC1 coding region containing the FNP in the japonica rice group. However, no selection occurred in the indica and wild-rice groups. A phylogenetic tree derived from TAC1 sequence analysis suggests that the indica and japonica subspecies arose independently during the domestication of wild rice.


Assuntos
Evolução Molecular , Genes de Plantas/genética , Oryza/genética , Mutação/genética , Oryza/crescimento & desenvolvimento , Filogenia , Polimorfismo Genético/genética , Seleção Genética , Especificidade da Espécie
13.
Int Orthop ; 36(1): 207-13, 2012 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-21674292

RESUMO

PURPOSE: The lysyl oxidase (LOX) family plays a crucial role in the formation and stabilisation of extracellular matrix (ECM) by catalysing the cross-linking of collagen and elastin, implicating its important fundamental roles in injury healing. A high level of transforming growth factor-ß(1) (TGF-ß(1)) accompanies the inflammatory phase of an injury of the knee joint. Our purpose was to detect the expressions of the LOX family in anterior cruciate ligament (ACL) and medial collateral ligament (MCL) response to TGF-ß(1). METHODS: This study used reversed transcript PCR, real time quantitative PCR and Western blot for analyses. RESULTS: The results showed significant increases in mRNA levels of LOX family members. At 5 ng/ml concentration of TGF-ß(1,) the gene profiles of LOXs showed most active, and LOX and LOXL-3 showed increasing peaks at 12 hours after TGF-ß(1) treatment (LOX: 7.2, 8.8-fold and LOXL-3: 3.8, 5.4-fold compared with normal controls in ACL and MCL, respectively); LOXL-1, LOXL-2 and LOXL-4 reached their highest amounts at six hours (LOXL-1: 1.9, 2.4-fold; LOXL-2: 14.8, 16.2-fold; LOXL-4: 2.5, 4.4-fold in ACL and MCL, respectively). Protein assays revealed that LOXs in ACL cells had relatively lower response to TGF-ß(1) compared with those in MCL cells. CONCLUSIONS: The differential expression and activities of LOXs might help to explain the intrinsic difference between ACL and MCL, and LOXs could imply a potential capability for ACL healing.


Assuntos
Ligamento Cruzado Anterior/citologia , Fibroblastos/citologia , Ligamento Colateral Médio do Joelho/citologia , Proteína-Lisina 6-Oxidase/genética , Fator de Crescimento Transformador beta/farmacologia , Regulação para Cima/efeitos dos fármacos , Adulto , Animais , Western Blotting , Células Cultivadas , Relação Dose-Resposta a Droga , Feminino , Fibroblastos/efeitos dos fármacos , Fibroblastos/enzimologia , Regulação da Expressão Gênica/efeitos dos fármacos , Humanos , Masculino , Camundongos , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Proteína-Lisina 6-Oxidase/metabolismo
14.
Sheng Wu Gong Cheng Xue Bao ; 27(10): 1417-27, 2011 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-22260058

RESUMO

Microfluidics deals with the manipulation of fluidics in the structure with dimensions of micrometers or nanometers. As an emerging field, microfluidics has numerous advantages, such as controllable fluid flow and reduced reagents consumption. Recently, microfluidic has been applied into the area of cell transfection, providing opportunities to investigate cell transfection process on microscale. This review summarizes recent technical development of cell transfection based on microfluidics, including transfeceted microarray, transfecetion established in miniaturization flowing space, microdrops, microinjection and microfluidic electroporation. The factors that affect the transfection efficiency and improvement approaches are also discussed.


Assuntos
Técnicas Analíticas Microfluídicas , Transfecção , Eletroporação , Técnicas Analíticas Microfluídicas/instrumentação , Miniaturização , Análise de Sequência com Séries de Oligonucleotídeos
15.
Mol Cell Biomech ; 7(2): 115-24, 2010 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-20936743

RESUMO

Human anterior cruciate ligament (ACL) has poor healing ability after injury. The devastating effects of matrix metalloproteinases (MMPs) excess expression are regarded as the main reason. Tissue inhibitor metalloproteinases (TIMPs) may be independent of MMPs inhibition. In this paper, a rat ACL rotating injury apparatus was designed to produce ACL injury. After inducing injury, joint fluids and ACL tissue total proteins were immediately extracted. In addition, ACL tissue was isolated in a culture plate with 1%FBS medium for the ex vivo study. We found MMP-2 in joint fluids increased significantly by 4 folds after ACL injury as a function of time. Ex vivo study showed MMP-2 in the medium and ACL cultured tissue increased significantly respectively to 3 folds and to 6 folds. The joint fluids global MMP increased to 3.5 folds with non-treatment and APMA-treatment in day three. On the gene expression level, the changes in MMP-1 and CD147 have the similar tendency. The ratio of MMP-1/TIMP-1 increased with time after ACL injury. We conclude that MMP-2 increased significantly in the early phase in the joint cavity after ACL injury. The ex vivo study demonstrated the same tendency. Generic MMP Activity Assay (global MMP assay) an dzymography also showed significant increase in MMP activity in joint fluids. These results showed ACL having poor healing ability after injury may not be only due to ACL release of large quantities of MMPs. The important factor may be the alterations in the whole joint cavity's internal environment.


Assuntos
Lesões do Ligamento Cruzado Anterior , Ligamento Cruzado Anterior/metabolismo , Metaloproteinases da Matriz/metabolismo , Inibidores Teciduais de Metaloproteinases/metabolismo , Animais , Sequência de Bases , Fenômenos Biomecânicos , Primers do DNA/genética , Modelos Animais de Doenças , Expressão Gênica , Humanos , Técnicas In Vitro , Masculino , Metaloproteinase 1 da Matriz/genética , Metaloproteinase 1 da Matriz/metabolismo , Metaloproteinase 2 da Matriz/genética , Metaloproteinase 2 da Matriz/metabolismo , Metaloproteinases da Matriz/genética , Ratos , Ratos Sprague-Dawley , Líquido Sinovial/metabolismo , Inibidor Tecidual de Metaloproteinase-1/genética , Inibidor Tecidual de Metaloproteinase-1/metabolismo , Inibidores Teciduais de Metaloproteinases/genética
16.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi ; 27(3): 675-9, 2010 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-20649042

RESUMO

Microfluidic chip is a novel technology platform, in which microchannels are fabricated in different materials. The ability to precisely control the microflows makes it possible to mimic the microenvironment of cells in physiological or pathological states, which provides many distinct advantages for cell research. In this paper are reviewed the design and fabrication of microfluidic chip, the application of microfluidic chip in cell culture and cell researches; the enormous advantages of microfluidic chips in precise experimental control of the cellular microenvironment are introduced.


Assuntos
Microambiente Celular , Técnicas Analíticas Microfluídicas , Adesão Celular , Técnicas de Cultura de Células , Movimento Celular , Células Cultivadas , Humanos , Técnicas Analíticas Microfluídicas/métodos
17.
Exp Cell Res ; 316(20): 3329-41, 2010 Dec 10.
Artigo em Inglês | MEDLINE | ID: mdl-20184882

RESUMO

Human bone marrow mesenchymal stromal cells (hBMSC) have been shown to participate in malignant transformation. However, hampered by the low frequency of malignant transformation of hBMSC, we do not yet know how to prevent malignant transformation of implanted hBMSC. In this study, in order to establish a model for the eradication of hBMSC-derived malignant cells, a gene fusion consisting of a human telomerase (hTERT) promoter modified with both c-Myc and myeloid zinc finger protein2 (MZF-2) binding elements and followed by the E. coli cytosine deaminase (CD) and luciferase genes was stably transferred into hBMSC via lentiviral transduction; n-phosphonacelyl-L-aspartic acid (PALA) selection was used to generate malignant cell colonies derived from transduced hBMSC after treatment with the carcinogenic reagent BPDE. Cells that were amplified after PALA selection were used for transplantation and 5-FC pro-drug cytotoxicity tests. The results showed that PALA-resistant malignant cells could be generated from hBMSC co-induced with lentiviral transduction and treatment with Benzo(a)pyrene Diol Epoxide (BPDE); the modification of c-Myc and MZF-2 binding elements could remarkably enhance the transcriptional activities of the hTERT promoter in malignant cells, whereas transcriptional activity was depressed in normal hBMSC; malignant cells stably expressing CD under the control of the modified hTERT promoter could be eliminated by 5-FC administration. This study has provided a method for targeted eradication of malignant cells derived from hBMSC.


Assuntos
Antimetabólitos/farmacologia , Células da Medula Óssea/citologia , Transformação Celular Neoplásica/induzido quimicamente , Células-Tronco Mesenquimais/citologia , Células-Tronco Neoplásicas/efeitos dos fármacos , Células Estromais/citologia , 7,8-Di-Hidro-7,8-Di-Hidroxibenzo(a)pireno 9,10-óxido/farmacologia , Adulto , Animais , Antígenos CD/metabolismo , Antimetabólitos/uso terapêutico , Ácido Aspártico/análogos & derivados , Ácido Aspártico/farmacologia , Diferenciação Celular/fisiologia , Sobrevivência Celular/efeitos dos fármacos , Transformação Celular Neoplásica/metabolismo , Citosina Desaminase/genética , Proteínas de Escherichia coli/genética , Flucitosina/farmacologia , Flucitosina/uso terapêutico , Expressão Gênica/genética , Genes Reporter/genética , Vetores Genéticos/genética , Proteínas de Fluorescência Verde/genética , Proteínas de Fluorescência Verde/metabolismo , Humanos , Fatores de Transcrição Kruppel-Like/genética , Fatores de Transcrição Kruppel-Like/metabolismo , Lentivirus/genética , Luciferases/genética , Masculino , Células-Tronco Mesenquimais/metabolismo , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , Células-Tronco Neoplásicas/citologia , Células-Tronco Neoplásicas/metabolismo , Células-Tronco Neoplásicas/transplante , Ácido Fosfonoacéticos/análogos & derivados , Ácido Fosfonoacéticos/farmacologia , Regiões Promotoras Genéticas/genética , Proteínas Proto-Oncogênicas c-bcl-2/genética , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Proteínas Proto-Oncogênicas c-myc/genética , Proteínas Proto-Oncogênicas c-myc/metabolismo , Elementos de Resposta/genética , Telomerase/genética , Transdução Genética , Proteína Supressora de Tumor p53/genética , Proteína Supressora de Tumor p53/metabolismo , Ensaios Antitumorais Modelo de Xenoenxerto , Adulto Jovem
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA