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2.
Small Methods ; : e2201260, 2023 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-36617685

RESUMO

Elucidation of hot carrier transport and cooling mechanisms at the micro-/nanoscale is critical for optoelectronics, thermal management, and photocatalysis. Spatiotemporal evolution of hot electrons is usually convoluted with their ultrafast dynamics. Herein, an ultrafast microscopy is employed to directly track the spatiotemporal distribution of photoexcited hot electrons, providing a transformative approach to unravel the competitive relationship of transport and cooling. In the temporal evolution profiles of hot electron distribution, an anomalous contracting stage showing obvious thickness and fluence dependency is observed, with a characteristic end time indicating the completion of electron-phonon (e-ph) thermalization. Hot electron transport plays a prominent role in the competition with e-ph coupling, while interfacial heat dissipation dominates nonequilibrium state evolution with thickness below ballistic length. This work significantly enriches the tool kit of ultrafast techniques and provides guidance for rational design and optimization of micro-/nanodevices.

3.
Clin Transl Sci ; 2023 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-36645160

RESUMO

Sepsis accounts for one in three hospital deaths. Higher concentrations of high-density lipoprotein cholesterol (HDL-C) are associated with apparent protection from sepsis, suggesting a potential therapeutic role for HDL-C or drugs, such as cholesteryl ester transport protein (CETP) inhibitors that increase HDL-C. However, these beneficial clinical associations might be due to confounding; genetic approaches can address this possibility. We identified 73,406 White adults admitted to Vanderbilt University Medical Center with infection; 11,612 had HDL-C levels, and 12,377 had genotype information from which we constructed polygenic risk scores (PRS) for HDL-C and the effect of CETP on HDL-C. We tested the associations between predictors (measured HDL-C, HDL-C PRS, CETP PRS, and rs1800777) and outcomes: sepsis, septic shock, respiratory failure, and in-hospital death. In unadjusted analyses, lower measured HDL-C concentrations were significantly associated with increased risk of sepsis (p = 2.4 × 10-23 ), septic shock (p = 4.1 × 10-12 ), respiratory failure (p = 2.8 × 10-8 ), and in-hospital death (p = 1.0 × 10-8 ). After adjustment (age, sex, electronic health record length, comorbidity score, LDL-C, triglycerides, and body mass index), these associations were markedly attenuated: sepsis (p = 2.6 × 10-3 ), septic shock (p = 8.1 × 10-3 ), respiratory failure (p = 0.11), and in-hospital death (p = 4.5 × 10-3 ). HDL-C PRS, CETP PRS, and rs1800777 significantly predicted HDL-C (p < 2 × 10-16 ), but none were associated with sepsis outcomes. Concordant findings were observed in 13,254 Black patients hospitalized with infections. Lower measured HDL-C levels were significantly associated with increased risk of sepsis and related outcomes in patients with infection, but a causal relationship is unlikely because no association was found between the HDL-C PRS or the CETP PRS and the risk of adverse sepsis outcomes.

4.
Mikrochim Acta ; 190(2): 52, 2023 01 14.
Artigo em Inglês | MEDLINE | ID: mdl-36639422

RESUMO

Based on a dual signal amplification strategy of novel accordion-like Bi2O3-decorated Ti3C2 (Ti3C2@Bi2O3) nanocomposites and hybridization chain reaction (HCR), an ultra-sensitive electrochemical biosensor was constructed for miRNA-21 detection. By etching Ti3AlC2 with HF, Ti3C2 with an accordion-like structure was first obtained and subsequently covered by Bi2O3 nanoparticles (NPs), forming Ti3C2@Bi2O3. A layer of Au NPs was electrodeposited on the glassy carbon electrode coated with Ti3C2@Bi2O3, which not only significantly improved the electron transport capacity of the electrode but also greatly increased its surface active area. Upon the immobilization of the thiolated capture probe (SH-CP) on the electrode, the target miRNA-21 specifically hybridized with SH-CP and thus opened its hairpin structure, triggering HCR to form a long double strand with the primers H1 and H2. A large number of the electrochemical indicator molecules were thus embedded inside the long double strands to produce the desirable electrochemical signal at a potential of - 0.19 V (vs. Ag/AgCl). Such dual signal amplification strategy successfully endowed the biosensor with ultra-high sensitivity for miRNA-21 detection in a wide linear range from 1 fM to 100 pM with a detection limit as low as 0.16 fM. The excellent detection of miRNA-21 in human blood plasma displayed a broad prospect in clinical diagnosis. An ultra-sensitive electrochemical biosensor was successfully constructed for miRNA-21 detection in human blood plasma based on the dual signal amplification strategy of novel accordion-like Bi2O3 decorated Ti3C2 (Ti3C2@Bi2O3) nanocomposites and hybridization chain reaction.


Assuntos
Nanopartículas Metálicas , MicroRNAs , Humanos , Ouro/química , Técnicas Eletroquímicas/métodos , Titânio , Nanopartículas Metálicas/química
5.
J Pharm Sci ; 2023 Jan 24.
Artigo em Inglês | MEDLINE | ID: mdl-36706835

RESUMO

Container closure integrity testing (CCIT) is a critical step in ensuring package integrity and providing feedback on package designs. In practical applications, CCIT methods, namely physical and probabilistic methods, must be appropriately selected and validated to ensure their suitability for the intended use. However, the industry still lacks practical recommendations regarding the choice of CCIT methods and artificial leaks to set the acceptance criteria. The main reason is the lack of correlation between testing methods. Artificially introduced leak microholes are the only way to determine the sensitivity of a CCIT method and to implement the method correlation. However, the type of artificial leakage is a key factor because in most studies, leakage is described and valued using a single parameter, such as size. This can significantly affect the credibility of the relevant test results, especially in the case of microbial invasion, where the difference in test conditions and samples will severely affect the probability of microbial invasion. Therefore, it is vital to conduct a systematic study on the influence of leakage conditions on CCIT methods. In this study, the influence of the shapes of artificial leaks on the two kinds of testing methods was systematically studied based on a laser-drilled microhole-a highly potential and non-exogenous artificial leak manufacturing method that can fabricate different leakage geometries. The reason for the influence of the shape of an artificial leak on the CCIT is that the deterministic method takes defects as an idealized model and ignores the influence of the leak shape, wall thickness, and other factors on leakage and pollution risks. However, these factors seriously affect the dynamic process of leakage and microbial invasion. The pressure decay method is used to test the leakage flow rate of conical and straight holes. Microbial challenge tests are then used to verify the impact of leakage shapes on the pollution risk. The results of the tests indicated that the probability of microbial invasion in the conical holes is much higher than that in straight holes with the same flow test results and that the wall thickness can also affect microbial invasion. Thus, it can be proven that the risk of leakage and invasion or the sensitivity of different methods cannot only be compared through the leak diameter. Numerous influencing factors, including leakage geometry (e.g., shape and thickness), must be considered in practical applications.

6.
PLoS Genet ; 19(1): e1010571, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36689473

RESUMO

Drosophila trachea is a premier model to study tube morphogenesis. After the formation of continuous tubes, tube maturation follows. Tracheal tube maturation starts with an apical secretion pulse that deposits extracellular matrix components to form a chitin-based apical luminal matrix (aECM). This aECM is then cleared and followed by the maturation of taenidial folds. Finally, air fills the tubes. Meanwhile, the cellular junctions are maintained to ensure tube integrity. Previous research has identified several key components (ER, Golgi, several endosomes) of protein trafficking pathways that regulate the secretion and clearance of aECM, and the maintenance of cellular junctions. The Osiris (Osi) gene family is located at the Triplo-lethal (Tpl) locus on chromosome 3R 83D4-E3 and exhibits dosage sensitivity. Here, we show that three Osi genes (Osi9, Osi15, Osi19), function redundantly to regulate adherens junction (AJ) maintenance, luminal clearance, taenidial fold formation, tube morphology, and air filling during tube maturation. The localization of Osi proteins in endosomes (Rab7-containing late endosomes, Rab11-containing recycling endosomes, Lamp-containing lysosomes) and the reduction of these endosomes in Osi mutants suggest the possible role of Osi genes in tube maturation through endosome-mediated trafficking. We analyzed tube maturation in zygotic rab11 and rab7 mutants, respectively, to determine whether endosome-mediated trafficking is required. Interestingly, similar tube maturation defects were observed in rab11 but not in rab7 mutants, suggesting the involvement of Rab11-mediated trafficking, but not Rab7-mediated trafficking, in this process. To investigate whether Osi genes regulate tube maturation primarily through the maintenance of Rab11-containing endosomes, we overexpressed rab11 in Osi mutant trachea. Surprisingly, no obvious rescue was observed. Thus, increasing endosome numbers is not sufficient to rescue tube maturation defects in Osi mutants. These results suggest that Osi genes regulate other aspects of endosome-mediated trafficking, or regulate an unknown mechanism that converges or acts in parallel with Rab11-mediated trafficking during tube maturation.


Assuntos
Proteínas de Drosophila , Drosophila , Animais , Drosophila/metabolismo , Traqueia/metabolismo , Proteínas rab de Ligação ao GTP/metabolismo , Endossomos/metabolismo , Proteínas de Drosophila/genética
7.
Life Sci ; 316: 121433, 2023 Jan 25.
Artigo em Inglês | MEDLINE | ID: mdl-36708987

RESUMO

The organellar Ca2+-activated K+ channels share a similar ability to transfer the alteration of Ca2+ concentration to membrane conductance of potassium. Multiple effects of Ca2+-activated K+ channels on cell metabolism and complex signaling pathways during organ development have been explored. The organellar Ca2+-activated K+ channels are able to control the ionic equilibrium and are always associated with oxidative stress in different organelles and the whole cells. Some drugs targeting Ca2+-activated K+ channels have been tested for various diseases in clinical trials. In this review, the known roles of organellar Ca2+-activated K+ channels were described, and their effects on different diseases, particularly on diabetes, cardiovascular diseases, and neurological diseases were discussed. It was attempted to summarize the currently known operational modes with the involvement of organellar Ca2+-activated K+ channels. This review may assist scholars to more comprehensively understand organellar Ca2+-activated K+ channels and related diseases.

8.
Food Chem ; 408: 135190, 2023 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-36535187

RESUMO

Calcium has limited bioavailability because of the formation of calcium phosphate deposits in the gastrointestinal tract. In this study, we prepared a dextran-casein phosphopeptide (CPP)-Ca2+ delivery system and evaluated for Ca2+ binding mechanism, structure, stability, and sustained release of Ca2+ and assessed inhibition of calcium phosphate precipitation. The results revealed that Ca2+ binds to dextran-CPP through the phosphate, carboxyl, and amino groups and forms crystal clusters. Furthermore, compared with single polymer CPP-Ca2+ conjugates, copolymer dextran-CPP-Ca2+ conjugates exhibited improved stability at various conditions (pH, temperature, and coexisting food), efficiently reduced the calcium phosphate precipitation, and improved sustained-release of Ca2+. Collectively, dextran-CPP-Ca2+ conjugates can be an efficient Ca2+ delivery system.


Assuntos
Cálcio , Dextranos , Cálcio/química , Caseínas/química , Fosfatos de Cálcio , Fosfopeptídeos/química
9.
Front Pediatr ; 10: 1005335, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36458147

RESUMO

Background: Auditory neuropathy (AN) is a hearing disorder caused by the failure of inner hair cells, auditory nerve synapses and/or auditory nerves. With the development of high-throughput sequencing technology, the genetic factors of AN have been revealed, and genetic testing has become an important tool for identifying different types of AN. Case description: To study the genetic cause of nonsyndromic auditory neuropathy in a Chinese family. The family was from Henan Province with three affected individuals. The audiological examinations were performed on the affected individuals, and whole-exome sequencing was carried out on the proband. The suspected pathogenic variants screened by the bioinformatic analysis were validated using Sanger sequencing in the family members. We identified three novel variants c.3277G > A (p.Glu1093Lys), c.4024-4G > T, and c.898-2A > G of the OTOF gene in the three children with AN. The first two variants were inherited from their father, and the third variant was inherited from their mother. A minigene assay was designed to test the effect of c.4024-4G > T on splicing. The variants c.3277G > A, c.4024-4G > T, and c.898-2A > G could be classified as likely pathogenic/pathogenic following the ACMG guidelines, and they are considered as the genetic causes for the patients in the family. Conclusion: New pathogenic/likely pathogenic variants of the OTOF gene were identified in a family with AN, enriching the mutational spectrum of the OTOF gene.

10.
Artigo em Inglês | MEDLINE | ID: mdl-36525187

RESUMO

People pose a serious risk by plants contaminated with lead in soil. However, the strength of lead enrichment capacity in root, stem, and leaf of the plant is still controversial. Therefore, a meta-analysis was conducted to investigate the ability of lead enrichment of root, stem, and leaf and the main influencing factors for lead absorption. The results of this study indicated that all parts of plant can significantly accumulate lead. Concentrations of lead followed an order of root > stem > leaf. Alkaline soil was conducive to the absorption of lead. When the lead concentration in the soil was higher than 20 mg/kg, the lead absorption in root was more. Lead is absorbed most in trees and least in Gramineae. It is argued that this study is beneficial to select plants suitable for absorption of lead from polluted soil. This study also can help to clarify the influencing factors for lead enrichment in different parts of the plant.

11.
ACS Omega ; 7(46): 42256-42263, 2022 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-36440162

RESUMO

Miniaturized resistance-based portable bending sensors have been widely used for human health monitoring in recent years. Their sensitivities are defined by their resistance variations (ΔR/R), which strongly rely on the conductivity and minimum line width of the sensing unit. Laser-induced carbonization is a fast and simple method to fabricate porous-sensing structures. However, the fabrication resolution of conductive and deformation-sensitive structures is limited by the thermal effect of commonly used laser sources. With the assistance of femtosecond laser temporal shaping, plasma ejection confinement, and silver nitrate doping, the sheet resistance of the sensing structure was improved from 15 to 0.0004 Ω/□. A thin line with a lateral resolution of 6.5 µm is fabricated as the sensing unit. The fFabricated structures are characterized by electron microscopy, Raman spectroscopy, energy-dispersive spectroscopy, X-ray scattering, and time-resolved images. The strain sensor demonstrates a ΔR/R of 25.8% with a rising edge of 109 ms in the cyclic bending test. The sensor is further applied for detecting human pulse and finger bending.

12.
J Nutr Biochem ; 112: 109214, 2022 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-36370928

RESUMO

Observational study indicated that folic acid (FA) supplementation may protect against tuberculosis-drug-induced liver injury (TBLI). The aim is to investigate the effect and mechanism of FA on TBLI in rats. Liver injury was induced by a daily gavage of isoniazid (INH) and rifampicin (RIF) in the model and FA groups. Rats in the FA group were also treated with 2.5 mg/kg body weight FA. Rats in the control group were not treated. Eight rats were used in each group. The severity of liver injury was measured by the serum levels of hepatic enzymes and histological score. The metabolites in serum and liver tissues were analyzed by HPLC-Q-TOF-MS/MS. FA treatment significantly reduced alanine aminotransferase and liver necrosis. Seventy-nine differential metabolites in the serum and liver tissues were identified among the three groups. N-acylethanolamines, INH and RIF metabolites, phosphatidylcholines, lysophosphatidylcholines, monoglycerides, diglycerides and bile acids were regulated by FA treatment, involving key metabolic pathways, such as N-acylethanolamine metabolism, INH and RIF metabolism, liver regeneration, inflammation alleviation and bile acid metabolism. RT-PCR and western blotting results confirmed the altered N-acylethanolamine metabolism and improved drug metabolism by FA. In conclusion, FA was protective against TBLI, which may be related to the regulation of N-acylethanolamine metabolism and drug detoxification by FA.

13.
Front Plant Sci ; 13: 1015114, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36340397

RESUMO

Reproductive growth and vegetative growth are a pair of main contradictions in the process of plant growth. Flowering, as part of reproductive growth, is a key switch in the life cycle of higher plants, which affects the yield and economic benefits of plants to a certain extent. The Euphorbiaceae species, including castor bean (Ricinus communis), physic nut (Jatropha curcas), tung tree (Vernicia fordii), cassava (Manihot esculenta), and rubber tree (Hevea brasiliensis), have important economic values because they are raw materials for the production of biodiesel, rubber, etc. The flowering mechanisms are still excluded in the Euphorbiaceae species. The flowering-related genes of Arabidopsis thaliana (Arabidopsis) were used as a reference to determine the orthologs of these genes in Euphorbiaceae genomes. The result showed that 146, 144, 114, 114, and 149 of 207 A. thaliana genes were respectively matched to R. communis, V. fordii, J. curcas, H. brasiliensis, and M. esculenta. These identified genes were clustered into seven pathways including gibberellins, floral meristem identity (FMI), vernalization, photoperiod, floral pathway integrators (FPIs), and autonomous pathways. Then, some key numbers of flowering-related genes are widely conserved in the Euphorbiaceae genomes including but not limited to FPI genes LFY, SOC1, FT, and FMI genes AG, CAL, and FUL. However, some genes, including FRI, FLC, and GO, were missing in several or all five Euphorbiaceae species. In this study, we proposed the putative mechanisms of flowering-related genes to control flowering and provided new candidate flowering genes for using marker-assisted breeding to improve variety quality.

14.
Fa Yi Xue Za Zhi ; 38(4): 526-532, 2022 Aug 25.
Artigo em Inglês, Chinês | MEDLINE | ID: mdl-36426699

RESUMO

The oral cavity is the second largest microbial bank in humans after the intestinal canal, colonizing a large number of microorganisms including viruses, bacteria, archaea, fungi and protozoa. The great number of microbial cells, good DNA stability, and individual has a unique microbial community, these characteristics make the human microbiome expected to become a new biomarker for forensic individual identification. This article describes the characteristics of human oral microorganisms and microbial molecular markers in detail, analyzes the potential application value of microorganisms in forensic individual identification, and reviews the research progress of human oral microorganisms in forensic individual identification.


Assuntos
Microbiota , Humanos , Medicina Legal
15.
Pharmacol Ther ; 241: 108314, 2022 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-36427568

RESUMO

Diabetic nephropathy is one of the most common complications in diabetes. It has been shown to be the leading cause of end-stage renal disease. However, due to their complex pathological mechanisms, effective therapeutic drugs other than angiotensin-converting enzyme inhibitors (ACEIs) and angiotensin receptor blockers (ARBs), which have been used for 20 years, have not been developed so far. Recent studies have shown that diabetic nephropathy is characterized by multiple signalling pathways and multiple targets, including inflammation, apoptosis, pyroptosis, autophagy, oxidative stress, endoplasmic reticulum stress and their interactions. It definitely exacerbates the difficulty of therapy, but at the same time it also brings out the chance for natural products treatment. In the most recent two decades, a large number of natural products have displayed their potential in preclinical studies and a few compounds are under invetigation in clinical trials. Hence, many compounds targeting these singals have been emerged as a comprehensive blueprint for treating strategy of diabetic nephropathy. This review focuses on the cellular and molecular mechanisms of natural prouducts that alleviate this condition, including preclinical studies and clinical trials, which will provide new insights into the treatment of diabetic nephropathy and suggest novel ideas for new drug development.

16.
Materials (Basel) ; 15(20)2022 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-36295282

RESUMO

Aluminum dross (AD) is a waste product produced during aluminum processing and can be used to prepare mullite ceramic materials. However, the research on the preparation of mullite porous ceramics entirely from solid waste is still in the development stage. In this paper, porous mullite ceramics were successfully fabricated using a solid-phase sintering process with AD and different silicon sources (fly ash, silica dust, and gangue) as raw materials. The bulk density, apparent porosity, and compressive strength of the specimens were obtained, and the phase compositions and microstructures of the sintered specimens were measured using XRD and SEM, respectively. The average activation energy of the phase transition of fly ash, silica dust, and gangue as silicon sources were 984 kJ/mol, 1113 kJ/mol, and 741 kJ/mol, respectively. The microstructures of the mullite in the specimens were prisms, random aggregates, and needle-shaped, respectively. The formation of needle-shaped mullite combined with the substrate enhanced the mechanical strength of the porous mullite ceramics. The apparent porosity, density, and compressive strength of the specimens with gangue as the silicon source were 33.13%, 1.98 g/cm3, and 147.84 MPa, respectively, when sintered at 1300 °C for 2 h.

17.
Colloids Surf B Biointerfaces ; 220: 112924, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36308884

RESUMO

A new sandwich-type electrochemical immunosensor for the sensitive detection of carcinoembryonic antigen (CEA) was originally developed using a unique bismuth (Bi)-gold (Au) nano-electrocatalyst triggering efficient capture of tumor marker, where the nano-electrocatalyst was obtained by implanting Au nanoparticles (Au NPs) inside the mesoporous NBiOF nanospheres (Au@NBOF NSs) for the purpose to marker secondary antibody (Ab2) and amplify the response signal through electrochemically catalyzing the reduction of hydrogen peroxide. The synergistic interaction between NBOF NSs and Au NPs endowed the as-received Au@NBOF nano-electrocatalyst with large electrocatalytic active surface area and powerful signal amplification function as upper sandwich layer to conjugate Ab2. The multi-walled carbon nanotubes sparkled with Au nanostars served as the lower sandwich layer to capture the primary antibody (Ab1), which enhanced the interfacial electron transport and the load capacity of Ab1 as a result of increasing the sensing response of the designed immunosensor based on the sandwich-type Ab1-CEA-Ab2 interaction. Such immunosensor proposed on the above double signal amplification strategy efficiently detected the target CEA in a wide concentration range from 100 fg mL-1 to 200 ng mL-1. The detection limit was as low as 9.57 fg mL-1 with excellent specificity and reproducibility. The satisfactory results in analyzing human serum samples indicate the potential application of this new immunosensor in early clinical diagnosis of cancer and the evaluation of treatment efficiency.


Assuntos
Técnicas Biossensoriais , Grafite , Nanopartículas Metálicas , Nanotubos de Carbono , Humanos , Ouro , Antígeno Carcinoembrionário , Bismuto , Técnicas Eletroquímicas/métodos , Biomarcadores Tumorais , Reprodutibilidade dos Testes , Imunoensaio/métodos , Limite de Detecção , Anticorpos Imobilizados
18.
Plant Divers ; 44(5): 445-454, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-36187548

RESUMO

The survival rate of Castanopsis kawakamii from seed to seedling is relatively low, leading to difficulties in the regeneration of its natural forests. Forest gaps play a vital role in plant regeneration and biodiversity maintenance in forest ecosystems. Unfortunately, our understanding of the effects of gap size and within-gap position on the seed germination and radicle growth of C. kawakamii is still limited. In particular, our knowledge on the relationship between gap size and environmental factors and their influence on seed germination and radicle growth is incomplete. In the present study, we studied the influences of forest gaps and within-gap position on seed regeneration on the germination and radicle growth of an endangered species C. kawakamii in a subtropical natural forest in China. We selected three large gaps (LG, gap size above 200 m2), three medium gaps (MG, gap size 50-100 m2), three small gaps (SG, gap size 30-50 m2), and non-gap (NG), and planted the seeds of C. kawakamii in five positions within each gap. The results showed that (1) the influence of forest gaps on seed germination rate was, from highest to lowest, medium gaps (51%), non-gap (47%), small gaps (40%) and large gaps (17%), and the seed germination rate was the highest in all positions in medium gaps, with the exception of the east position. (2) Radicle length in forest gaps was, from highest to lowest, medium gaps, small gaps, large gaps and non-gap, and it was the highest in the east, south, west and north positions of medium gaps. (3) Canopy openness (gap size) and air temperature were the main factors influencing seed germination and radicle growth of C. kawakamii. We concluded that medium-sized gaps were the most suitable for seed germination and radicle growth of C. kawakamii, and they promote the regeneration of this endangered species in the investigated natural forest.

19.
Phytomedicine ; 107: 154476, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36191551

RESUMO

BACKGROUND: Baicalin, a flavonoid glycoside isolated from Scutellaria baicalensis Georgi, has shown potential pharmacological effects on myocardial ischemia diseases. Nevertheless, systematic preclinical studies on baicalin in the treatment of ischemic diseases are scarce. PURPOSE: To assess the efficacy and potential mechanisms of baicalin in myocardial ischemia (RI), myocardial ischemia-reperfusion (IR) injury and myocardial infarction (MI) animal models for future clinical research. METHODS: Preclinical studies published prior to August 27th, 2022 were retrieved from PubMed, Embase, Web of Science and Cochrane Library. CAMARADES list was used to evaluate the quality of included researches. Meta-analyses of cardiac pathology and function parameters, myocardial injury markers and other indicators were performed by STATA 15.0 software. Potential mechanisms are categorized and summarized. Dose-response interval analyses were used to analyze the dose-response relationship between baicalin and myocardial ischemia disease. RESULTS: Fourteen studies and 222 animals were included in the analysis. The results showed that compared with the control group, baicalin could reduce myocardial infarction size associated with cardiac pathological condition and the corresponding cardiac pathological index containing CK-MB, CK and cTnT. Additionally, heart function indicators including LVSP, LVFS, LVEF, -dp/dt max, dp/dt max were increased by baicalin. As for subgroup analyses, baicalin also demonstrated certain effect on CK-MB and LVSP by administration method or stage. Furthermore, it displayed obvious effect on myocardial ischemia diseases when the dose is maintained at 100-150 mg/kg based on dosage analyses. CONCLUSION: Based on the relevant literature retrieved, this is the first meta-analysis on baicalin in treating myocardial ischemia diseases. Notably, we linked the dynamic development of the disease and discussed it pertinently, from RI, IR injury to MI. Baicalin exhibits positive effects on myocardial ischemia diseases (especially when the dose is 100-150 mg/kg), which is achieved by regulating key pathological indicators and various signaling pathways.


Assuntos
Infarto do Miocárdio , Isquemia Miocárdica , Traumatismo por Reperfusão Miocárdica , Animais , Modelos Animais de Doenças , Flavonoides/farmacologia , Flavonoides/uso terapêutico , Glicosídeos , Infarto do Miocárdio/tratamento farmacológico , Isquemia Miocárdica/tratamento farmacológico , Traumatismo por Reperfusão Miocárdica/metabolismo
20.
ACS Appl Mater Interfaces ; 14(39): 44065-44083, 2022 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-36125961

RESUMO

Osteosarcoma is a devastating malignant neoplasm that seriously threatens human health. After an osteosarcoma resection, the simultaneous treatment of tumor recurrence, postoperative infection, and large bone loss remains a formidable challenge clinically. Herein, a versatile multiscale therapeutic platform (Fs-BP-DOX@PDA) is engineered based on NiTi alloys with versatile properties for near-infrared (NIR)-mediated osteosarcoma synergistic photothermo-chemotherapy, bone regeneration, and bacterial elimination. First, an intriguing method for fabricating groovelike micro-nanostructures (Fs-NiTi) through femtosecond laser direct writing to enhance osseointegration with strong contact guidance is proposed. Then, black phosphorus (BP) nanosheets as gratifying photothermal conversion agents, osteogenetic agents, and a drug delivery platform are decorated on Fs-NiTi to construct multiscale hierarchical structures (Fs-BP). Finally, the polydopamine (PDA) modification is utilized to enhance the photothermal performance, biocompatibility, and chemical stability of doxorubicin (DOX)-loaded Fs-BP and endow NIR/pH-dual-responsive DOX release properties. Fs-BP-DOX@PDA effectively induces tumor cell (Saos-2 and MDA-MB-231) death in vitro, completely eradicates osteosarcoma in mice, and observably promotes bone-regeneration bioactivity. Furthermore, it possesses prominent antibacterial efficiencies toward Staphylococcus aureus (99.2%) and Pseudomonas aeruginosa (99.6%). Overall, this work presents a smart comprehensive fabrication methodology to construct a versatile multiscale therapeutic platform for multimodal osteosarcoma treatment and biomedical tissue engineering.


Assuntos
Neoplasias Ósseas , Nanopartículas , Osteossarcoma , Ligas , Animais , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Neoplasias Ósseas/tratamento farmacológico , Linhagem Celular Tumoral , Doxorrubicina/uso terapêutico , Humanos , Camundongos , Nanopartículas/química , Osteossarcoma/tratamento farmacológico , Fósforo , Fototerapia
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