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1.
Int J Biol Macromol ; 138: 874-880, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31356945

RESUMO

A water-soluble polysaccharide (MBP-1) was isolated from Mung bean (Vigna radiate L.) and its physicochemical characterization, functional properties, and antioxidant activity was investigated. Chemical analysis revealed that the contents of carbohydrate, uronic acid, and protein were 33.05 ±â€¯0.10%, 25.83 ±â€¯0.04%, and 3.17 ±â€¯0.36%, respectively. The molecular weight (Mw) of MBP-1 was 146.22 kDa and it was consist of arabinose (Ara), glucose (Glc), xylose (Xyl), galactose (Gal), and galacturonic acid (GlcA) in a molar ratio of 0.94:0.37:0.33:0.13:5.99. The results of X-ray diffractometry (XRD) and thermal gravimetric analysis (TGA) indicated MBP-1 was semi-crystalline material with better thermal stability. MBP-1 exhibited good hydration properties and oil holding capacity. In addition, the antioxidant assay results showed MBP-1 had high 1,1-Diphenyl-2-picrylhydrazylradical (DPPH) and hydroxyl radical scavenging activities with concentration for 50% of maximal effect (EC50) of 1.59 and 0.51 mg/mL. Overall, these results indicated that MBP-1 presents a promising natural source of food additive or antioxidant.

2.
Bioresour Technol ; 287: 121467, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31121447

RESUMO

In this study, three sequencing batch reactors Ra, Rb, Rc with different inoculum sources (activated sludge; activated sludge plus detergent degrading consortium; detergent degrading consortium) were used to treat detergent wastewater [consisting of sodium dodecyl sulfate, polyoxyethylene lauryl ether and tetrasodium ethylenediamine tetraacetate (Na4EDTA)]. Fast start-up and highest performance in phase I and II (organic loading rate were 0.28, 0.39 kgCOD/kgMLSS/d, respectively) were observed in Rc. In contrast, Rb showed highest impact resistance to the increase of EDTA concentration in phase III. High-throughput sequencing analysis showed that inoculum sources led to significant differences on microbial community in phase I. However, regardless of the influent variation in phases II and III, the differences on microbial community among three SBRs were diminished along long-term operation. Pseudomonas, Sphingopyxis, Luteimonas, Pseudoxanthomonas and SM1A02 were found to be the core taxa, they might contribute to the excellent performance of detergent wastewater treatment.


Assuntos
Microbiota , Águas Residuárias , Reatores Biológicos , Detergentes , Esgotos , Eliminação de Resíduos Líquidos
3.
Carbohydr Polym ; 212: 270-276, 2019 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-30832857

RESUMO

In this study, the relaxation modulus was used to elucidate the gelling mechanism of polysaccharides from Mesona blumes. The pH of Mesona blumes polysaccharides (MBP) was adjusted could significantly change the relaxation modulus of MBP. The results showed that the hydrogen bonds and electrostatic interaction existed in during the formation of MBP gel. The addition of salt ions (sodium ions and calcium ions), EDTA and urea have different effects on the relaxation modulus of MBP. Result showed that the hydrogen bond was the main force maintaining the MBP gel network structure, followed was calcium ions. And electrostatic interaction was not the decisive role of gel formation. The small molecules with active hydrogen-bond donors and/or acceptors were added into MBP, which proved -COOH was involved in the hydrogen bonds formation of MBP gel. In addition, the entanglement network number (ENN) results quantitatively assessed contribution of interaction: hydrogen bonds interaction > calcium ions (calcium bridge) >electrostatic interaction.


Assuntos
Cálcio/química , Géis/química , Lamiaceae , Extratos Vegetais/química , Polissacarídeos/química , Ureia/química , Cálcio/metabolismo , Relação Dose-Resposta a Droga , Módulo de Elasticidade/fisiologia , Géis/metabolismo , Extratos Vegetais/isolamento & purificação , Extratos Vegetais/metabolismo , Polissacarídeos/isolamento & purificação , Polissacarídeos/metabolismo , Reologia/métodos , Ureia/metabolismo
4.
Int J Biol Macromol ; 126: 30-36, 2019 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-30584944

RESUMO

Mesona chinensis has been used as a Chinese folk medicine and main ingredient used to make "black bean jelly" in Asia for hundreds of years. In this study, two polysaccharides (MCP-C and MCP-S) from Mesona chinensis were extracted by using cellulase assisted extraction (CAE) and sodium carbonate assisted extraction (SAE), separately. Then the different physicochemical characteristics, rheological and thermal properties of two polysaccharides were analyzed by ion chromatography, high-performance gel permeation chromatography (HPGPC), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), rheological and thermogravimetric analysis. Results indicated that the contents of total sugar and uronic acid of MCP-C were higher than MCP-S, while the protein content and molecular weight was lower than that of MCP-S. MCP-S and MCP-C had differences in the surface morphology by SEM, while they all had the typical IR spectra characteristic and amorphous morphology of polysaccharides. The rheological experiments showed that MCP-C and MCP-S exhibited typical pseudoplasticity fluids behavior. The apparent viscosity (η), storage modulus (G') and complex viscosity (η*) of MCP-S were higher than MCP-C. The three-stage decomposition patterns were observed in MCP-C and MCP-S. MCP-S was more stable and had higher initial decomposition temperature (Ti) than MCP-C.


Assuntos
Carbonatos/química , Celulase/metabolismo , Lamiaceae/química , Polissacarídeos/química , Polissacarídeos/isolamento & purificação , Reologia , Temperatura Ambiente , Peso Molecular , Monossacarídeos/análise , Polissacarídeos/ultraestrutura , Espectrofotometria Ultravioleta , Espectroscopia de Infravermelho com Transformada de Fourier , Difração de Raios X
5.
Int J Biol Macromol ; 2018 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-30502438

RESUMO

Mesona blumes polysaccharide (MBP), a primary active component extracted from Mesona blumes, has a number of bioactivities. Nevertheless, hepatoprotective activity of MBP has been rarely reported. The purpose of this study is to investigate hepatoprotective effects of MBP on acute liver injury in mice. Results indicated that the MBP could remarkably decrease the increased levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in the serum caused by tetrachloride (CCl4) treatment (P < 0.05). Medium and high dose of MBP treatment (200 mg/kg body weight, 300 mg/kg body weight) not only prominently enhanced the levels of antioxidant enzymes (superoxide dismutase, SOD) and non-enzyme antioxidants (glutathione, GSH) compared with CCl4-induced, but also dramatically decreased lipid peroxidation levels of liver tissues (P < 0.05). In addition, medium and high doses of MBP significantly enhanced the serum levels of IL-1ß and TNF-α (P < 0.05). This study showed that MBP had hepatoprotective activity against acute liver injury caused by CCl4.

6.
Carbohydr Polym ; 200: 191-199, 2018 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-30177157

RESUMO

Dynamic high-pressure appears to be an alternative approach to physical modification of polysaccharides aimed to improve their functional characteristics. The objective of our study was to evaluate the effect of dynamic high-pressure microfluidization (DHPM) treatment on the physicochemical properties and antioxidant activities of Mesona chinensis Benth polysaccharide (MP). The results indicated that the contents of total sugar and uronic acid in DHPM-treated polysaccharide samples (DMP) were increased, and protein content in DMP was significantly decreased after DHPM treatment. The molecular weights of MP and DMP were 1.58 × 105 Da and 1.64 × 105 Da. MP and DMP were composed of Gal, Xyl, and GalA in a molar ratio of 2.8: 5.5: 2.4 and 3.8: 7.4: 3.0, respectively. The antioxidant activities of MP were slightly promoted after DHPM treatment. Moreover, DHPM treatment leads to changes in the morphology of polysaccharide. The surface appearances of DHPM-treated polysaccharide samples showed a slightly curly surface compared to original polysaccharide by SEM analysis. No considerable changes were observed in the structure between the MP and DMP by FT-IR. The results provide useful information for future application of MP, and show DHPM treatment can improve the antioxidant activity of polysaccharides.


Assuntos
Antioxidantes/química , Fenômenos Químicos , Lamiaceae/química , Microfluídica , Polissacarídeos/química , Polissacarídeos/farmacologia , Pressão , Antioxidantes/farmacologia , Compostos de Bifenilo/química , Peso Molecular , Monossacarídeos/análise , Picratos/química , Ácidos Urônicos/análise
7.
Gut Pathog ; 10: 37, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30214488

RESUMO

Background: Culture-based diagnostic methods cannot achieve rapid and precise diagnoses for the identification of multiple diarrhoeal pathogens (DPs). A high-throughput multiplex genetic detection system (HMGS) was adapted and evaluated for the simultaneous identification and differentiation of infectious DPs and a broad analysis of DP infection aetiology. Results: DP-HMGS was highly sensitive and specific for DP detection compared with culture-based techniques and was similar to singleplex real-time PCR. The uniform level of sensitivity of DP-HMGS for all DPs allowed us to remap the aetiology of acute diarrhoeal infections in Shanghai, correcting incidences of massively underdiagnosed DP species with accuracy approaching that of sequencing-based methods. The most frequent DPs were enteropathogenic Escherichia coli, rotavirus and Campylobacter jejuni. DP-HMGS detected two additional causes of infectious diarrhoea that were previously missed by routine culture-based methods: enterohemorrhagic E. coli and Yersinia enterocolitica. We demonstrated the age dependence of specific DP distributions, especially the distributions of rotavirus, intestinal adenovirus and Clostridium difficile in paediatric patients as well as those of dominant bacterial infections in adults, with a distinct "top 3" pattern for each age group. Finally, the multiplexing capability and high sensitivity of DP-HMGS allowed the detection of infections co-induced by multiple pathogens (approximately 1/3 of the cases), with some DPs preferentially co-occurring as infectious agents. Conclusions: DP-HMGS has been shown to be a rapid, specific, sensitive and appropriate method for the simultaneous screening/detection of polymicrobial DP infections in faecal specimens. Widespread use of DP-HMGS is likely to advance routine diagnostic and clinical studies on the aetiology of acute diarrhoea.

8.
Nurse Educ Today ; 71: 40-47, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30223169

RESUMO

BACKGROUND: The demand for more creative and innovative nurses and together with the rapid expansion of nurse education in Mainland China have called for new approaches to student learning. Challenge Based Learning, an active student-directed approach was incorporated into an elective course in an undergraduate nursing programme. Initially, the students were given some big ideas about the real world. They worked together in small groups to identify the most challenging problems from these ideas, develop some innovative solutions, formulate an action plan for a selected solution, carry out the actions and evaluate the outcome. OBJECTIVES: To evaluate the effectiveness of Challenge Based Learning on students' creativity and innovativeness. DESIGN: Quasi-experimental. SETTINGS: A medical university in Guangzhou, China. METHOD AND PARTICIPANTS: A convenience sample of 48 undergraduate nursing students from the bilingual class, who enrolled in the elective course, Innovation and Creative Thinking was invited to participate in the evaluative study. They completed the Creativity and Innovation Effectiveness Profile before and after the course. RESULTS: Apart from descriptive statistics, the mean scores of the Creativity and Innovation Effectiveness Profile between the pretest and posttest assessments were analyzed using the Wilcoxon signed ranks test. The results showed that the mean scores of all 7 domains (i.e. creative consciousness, levels of curiosity, pattern breaking skills, idea nurturing ability, willingness to experiment and take risks, courage and resilience and energetic persistence) of Creativity and Innovation Effectiveness were significantly higher in the posttest. CONCLUSION: Students can enhance their ability to innovate and create through learning. The successful practical experience of using CBL in the study provides a good reference for nurse teachers who want their students to be self-directed, creative and innovative.

9.
Nanomaterials (Basel) ; 8(5)2018 May 07.
Artigo em Inglês | MEDLINE | ID: mdl-29735918

RESUMO

The nanoemulsions of soy protein isolate-phosphatidylcholine (SPI-PC) with different emulsion conditions were studied. Homogenization pressure and homogenization cycle times were varied, along with SPI and PC concentration. Evaluations included turbidity, particle size, ζ-potential, particle distribution index, and turbiscan stability index (TSI). The nanoemulsions had the best stability when SPI was at 1.5%, PC was at 0.22%, the homogenization pressure was 100 MPa and homogenization was performed 4 times. The average particle size of the SPI-PC nanoemulsions was 217 nm, the TSI was 3.02 and the emulsification yield was 93.4% of nanoemulsions.

10.
Carbohydr Polym ; 192: 193-201, 2018 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-29691013

RESUMO

Effect of different salt ions on the gel properties and microstructure of Mesona blumes polysaccharide (MBP)-soy protein isolates (SPI) mixed gels were investigated. Sodium and calcium ions were chosen to explore their effects on the rheological behavior and gel properties of MBP-SPI mixed gels were evaluated by using rheological, X-ray diffraction, protein solubility determination, and microstructure analysis. Results showed that the addition of salt ions change the crystalline state of gels system, the crystal of gel was enhanced at low ion concentrations (0.005-0.01 M). The two peaks of gel characteristic at 8.9° and 19.9° almost disappeared at high salt ions concentrations (0.015-0.02 M), and new crystallization peaks appeared at around 30° and 45°. The elasticity, viscosity, gel strength, water holding capacity, and thermal stability of gel were increased at low ion concentration. Results showed that the main interactions which promoted gel formation and maintain the three-dimensional structure of the gel were electrostatic interactions, hydrophobic interactions, and disulfide interactions.


Assuntos
Cloreto de Cálcio/química , Lamiaceae/química , Cloreto de Sódio/química , Proteínas de Soja/química , Géis , Reologia
11.
J Am Soc Hypertens ; 12(2): 128-134, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29289467

RESUMO

The aim of this study was to assess the control of blood glucose, blood pressure (BP), serum low-density lipoprotein cholesterol (LDL-c), and other cardiovascular disease risk factors among patients with type II diabetes in a primary-care setting in Beijing. We performed a cross-sectional, multi-center survey of 4056 patients with type II diabetes aged ≥40 years. In total, 22.6% were current smokers, 10.8% often drank alcohol, 29.0% were obese, and 67.4% participated in adequate levels of physical activity. About 70% of patients reported comorbid hypertension or dyslipidemia. Of these, 70.8% were being treated for diabetes and 79.3% for hypertension; 20.5% were receiving statins and 28.5% aspirin. The proportions of patients achieving their therapeutic target were 52.6% for fasting plasma glucose, 58.2% for BP, and 33.0% for LDL-c. Only 11.1% achieved all three goals. Among 1960 (48.3%) patients with a record of hemoglobin A1C, 27.8% achieved the hemoglobin A1C target (<6.5%). These data suggest that blood glucose and BP were more likely to be well controlled than LDL-c, the likelihood of control of multiple risk factors is low, and that the statin and aspirin use should be intensified in patients with a substantial risk of cardiovascular disease.

12.
Nat Prod Res ; 32(5): 588-591, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-28431473

RESUMO

Osmanthus fragrans are well-known for their fragrance, but it is wasteful if to discard O. fragrans flower after extracting their essential oils. In this paper, we found that O. fragrans flower residues were rich in flavonoids. Six flavonoids and one phenylethanoid glycoside were isolated from the ethanol extract of O. fragrans flower residues, identified as quercetin (1), rutin (2), verbascoside (3), genistin (4), kaempferol (5), isorhamnetin (6) and naringin (7). In bioactivity study, kaempferol (IC50 = 1.43 µg/mL) showed the best anti-inflammatory activity. Isorhamnetin, quercetin, kaempferol, verbascoside and rutin (the values of IC50 were 18.30, 11.05, 16.88, 20.21 and 22.76 µg/mL, respectively) showed excellent DPPH free radical scavenging activity. Verbascoside performed relatively well at inhibiting the growth of both CT26 colonic carcinoma cells (IC50 = 46.87 µg/mL) and HepG2 hepatocarcinoma cells (IC50 = 30.58 µg/mL). In addition, quercetin and kaempferol showed strong anti-proliferation activity against HepG2 cells.


Assuntos
Flavonoides/química , Flavonoides/farmacologia , Óleos Voláteis/química , Oleaceae/química , Animais , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/farmacologia , Linhagem Celular , Fracionamento Químico , Avaliação Pré-Clínica de Medicamentos/métodos , Flores/química , Depuradores de Radicais Livres/química , Depuradores de Radicais Livres/farmacologia , Células Hep G2 , Humanos , Concentração Inibidora 50 , Quempferóis/química , Quempferóis/farmacologia , Camundongos , Estrutura Molecular , Óxido Nítrico/metabolismo , Óleos Voláteis/análise , Quercetina/química , Quercetina/farmacologia , Rutina/química , Rutina/farmacologia
13.
Nat Prod Res ; : 1-4, 2017 Nov 26.
Artigo em Inglês | MEDLINE | ID: mdl-29172783

RESUMO

The influence of natural drying (ND), hot-air drying (HD), vacuum drying (VD), infrared drying (ID) and freeze drying (FD) on bioactive compounds and bioactivities of Isodon rubescens (Hemsl.) was investigated in this study. The results showed that different drying methods resulted in the differences in bioactive compositions' content, antioxidant and antibacterial activities of extracts from I. rubescens. FD sample possessed the highest content of total phenolics, total flavonoids and several main phenolic compounds, as well as the stronger antioxidant and antibacterial activities, followed by ND, HD and VD, the lowest for ID samples. For this reason, freeze drying would seem to be more advisable for the drying I. rubescens, and future studies could focus on the quality evaluation and optimising various drying parameters.

14.
J Food Sci ; 82(6): 1319-1325, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28471049

RESUMO

Soybeans contain oil bodies (OBs) that encapsulate triacylglycerols (TAGs) with a phospholipid monolayer carrying scattered proteins. In nature, soybean OBs can form natural emulsions in aqueous media and may serve as natural, minimally processed, stable, and pre-emulsified oil for addition into appropriate food systems. In this study, OBs were obtained by aqueous extraction from the mature seeds of 2 soybean crop cultivars, high-fat soybean and low-fat soybeans. The compositions of the extracted OBs were analyzed during storage at room temperature up to 14 d (pH = 7). The oxidative stability of these OBs, stored at 60 °C, was evaluated by measuring the presence of primary (lipid hydroperoxides) and secondary lipid oxidation products (malondialdehyde) by determining the standard peroxide value (PV) and thiobarbituric acid-reactive substances (TBARS) value. During storage, the contents of unsaturated fatty acids, phospholipids, and tocopherols declined in both OBs, while their mean particle diameters (d32 ) and ζ-potentials increased. The changes in PV and TBARS values exhibited a similar trend for both OBs, but the OBs from low-fat soybeans had significantly lower PV and higher TBARS values than the OBs from high-fat soybean cultivars (P < 0.05). Overall, the OBs from both soybean cultivars had good stability during storage.


Assuntos
Gotículas Lipídicas/química , Óleo de Soja/química , Soja/química , Emulsões/química , Conservação de Alimentos , Oxirredução , Sementes/química , Substâncias Reativas com Ácido Tiobarbitúrico
15.
Ying Yong Sheng Tai Xue Bao ; 28(3): 829-837, 2017 Mar 18.
Artigo em Chinês | MEDLINE | ID: mdl-29741009

RESUMO

A series of typical abandoned croplands in the regions of Ruanliang and Yingliang in the Ordos Plateau, China, were selected, and dynamics of the surface litter, biological soil crust and soil bulk density, soil texture, and soil moisture in different soil layers were investigated. The results showed that in the abandoned cropland in Ruanliang, the clay particle content and surface litter of the surface soil layer (0-10 cm) increased during the restoration process, while that of soil bulk density substantially decreased and soil water content slightly increased in the surface soil. In the medium soil layer (10-30 cm), the clay particle content increased and the soil water content slightly decreased. In the deep soil layer (30-50 cm), there was a relatively large variation in the physical properties. In the abandoned cropland in Yingliang, the coverage of litter and the coverage and thickness of the biological soil crust increased during the abandonment process. The surface soil bulk density, soil clay particle content and soil water content remained constant in 0-10 cm soil layer, while the physical properties varied substantially in 10-40 cm soil layer. The shallow distribution of the soil water content caused by the accumulation of the litter and clay particles on the soil surface might be the key reason of the replacement of the semi-shrub Artemisia ordosica community with a perennial grass community over the last 20 years of the abandoned cropland in Ruanliang. The relatively high soil water content in the shallow layer and the development of the biological soil crust might explain why the abandoned cropland in Yingliang was not invaded by the semi-shrub A. ordosica during the restoration process.


Assuntos
Produtos Agrícolas , Solo , China , Ecossistema , Água
16.
J Zhejiang Univ Sci B ; 17(11): 882-891, 2016 Nov..
Artigo em Inglês | MEDLINE | ID: mdl-27819135

RESUMO

Camellia seed oil (CSO) is rich in oleic acid and has a high number of active components, which give the oil high nutritional value and a variety of biological activity. The aim of the present study was to determine the changes in the content and distribution of total polar compounds (TPC) in CSO during heating. TPC were isolated by means of preparative flash chromatography and further analyzed by high-performance size-exclusion chromatography (HPSEC). The TPC content of CSO increased from 4.74% to 25.29%, showing a significantly lower formation rate as compared to that of extra virgin olive oil (EVOO) and soybean oil (SBO) during heating. Furthermore, heating also resulted in significant differences (P<0.05) in the distribution of TPC among these oils. Though the content of oxidized triacylglycerol dimers, oxidized triacylglycerol oligomers, and oxidized triacylglycerol monomers significantly increased in all these oils, their increased percentages were much less in CSO than those in EVOO, indicating that CSO has a greater ability to resist oxidation. This work may be useful for the food oil industry and consumers in helping to choose the correct oil and to decide on the useful lifetime of the oil.


Assuntos
Camellia/química , Cromatografia em Gel/métodos , Óleos Vegetais/análise , Ácidos Graxos/análise , Calefação , Sementes/química , Fatores de Tempo
17.
Exp Ther Med ; 12(2): 746-752, 2016 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-27446270

RESUMO

The main function of adipocyte fatty acid-binding protein (A-FABP) is to regulate fatty acid metabolism as its molecular chaperone. The clinical significance of A-FABP in hypoxic-ischemic brain damage (HIBD) neonates is not yet clear. Free fatty acid (FFA) in cerebral cortex increases along with hypoxia ischemia degree. Thus, we aimed to investigate whether FFA can induce A-FABP expression and elevate the serum A-FABP level in HIBD neonates. In the present study, 42 HIBD neonates were selected including 11 cases as mild, 16 cases as moderate and 15 cases as severe. The serum was collected from peripheral vein at 72 h after the first visit (acute stage) and 7 days after birth (recovery stage), and the serum from 10 normal neonates was used as the control. The serum level of A-FABP and FFA in 42 neonates with acute phase and recovery phase HIBD were detected using ELISA and copper colorimetric method. The overall serum A-FABP content in HIBD neonates at the acute stage was significantly higher compared to the normal neonates (P<0.05). The serum A-FABP level in severe HIBD neonates was significantly higher than that in mild HIBD, moderate HIBD and normal neonates (P<0.05). The serum FFA level in HIBD neonates at the acute stage was 1,521.57±605.63 µmol/l, which was significantly higher than that in the normal neonates 838.24±294.22 µmol/l. The serum FFA levels in mild, moderate and severe HIBD neonates were significantly higher than those in the normal neonates. The overall A-FABP level in HIBD neonates at the recovery stage was significantly lower compared to the acute stage, which was significant in severe HIBD neonates. A-FABP levels in mild and moderate HIBD neonates at recovery stage were decreased compared with the acute stage, although there was no statistical difference. There was a positive correlation between serum A-FABP and FFA in HIBD neonates at acute stage (r=0.369, P<0.05). In conclusion, serum A-FABP and FFA levels were signifcantly increased in HIBD neonates at acute stage, and were positively correlated. The serum A-FABP level in HIBD neonates at recovery stage was significantly lower than that in the acute stage. The results suggested that serum A-FABP and FFA levels at acute stage can reflect the severity of HIBD. The detection of serum A-FABP and FFA can be applied as indicators for the early diagnosis of HIBD, but also provides a basis for the clinical evaluation of HIBD treatment.

18.
J BUON ; 21(2): 341-8, 2016 Mar-Apr.
Artigo em Inglês | MEDLINE | ID: mdl-27273943

RESUMO

PURPOSE: To evaluate the rate of overexpression of matrix metalloproteinase-2 (MMP2), mouse double minute 2 homolog (MDM2) and epidermal growth factor receptor (EGFR) in patients with non-small cell lung cancer (NSCLC), and evaluate their correlation with clinicopathological parameters and prognosis. METHODS: This was a prospective cohort study conducted from 2003 to 2008 among 184 NSCLC patients who underwent tumor resection. Each patient's clinical history and tumor characteristics were obtained from histopathology reports and medical records. EGFR, MDM2 and MMP2 expression were assessed by immunohistochemical (IHC) staining of the tissue specimens. RESULTS: MDM2 overexpression was observed in 70 (38%) of the patients studied, and was significantly higher in younger patients (p=0.01). Only 46 (25%) of patients had overexpression of MMP2. EGFR positive staining occurred in 105 (57%percnt;) of the evaluated tumor specimens and was more frequent in specimens with squamous cell carcinoma (p<0.001), the elderly (p<0.001), and in smokers (p<0.001). Independent risk factors for mortality were older age (adjusted odds ratio/aOR 1.3=), being a smoker (aOR 10), having stage II disease (aOR 10.8) or stage III/IV disease (aOR 28.3), expression of EGFR (aOR 5.9) and MMP2 (aOR 4.1). However, the expression of MDM2 independently predicted a reduced risk of death (aOR 0.3). CONCLUSION: Overexpression of MMP2 and EGFR were independent risk factors for mortality in NSCLC patients, while overexpression of MDM2 independently predicted a reduced risk of death.


Assuntos
Biomarcadores Tumorais/análise , Carcinoma Pulmonar de Células não Pequenas/química , Receptores ErbB/análise , Neoplasias Pulmonares/química , Metaloproteinase 2 da Matriz/análise , Proteínas Proto-Oncogênicas c-mdm2/análise , Adulto , Idoso , Idoso de 80 Anos ou mais , Carcinoma Pulmonar de Células não Pequenas/mortalidade , Carcinoma Pulmonar de Células não Pequenas/patologia , Carcinoma Pulmonar de Células não Pequenas/terapia , Distribuição de Qui-Quadrado , Feminino , Humanos , Imuno-Histoquímica , Modelos Logísticos , Neoplasias Pulmonares/mortalidade , Neoplasias Pulmonares/patologia , Neoplasias Pulmonares/terapia , Masculino , Pessoa de Meia-Idade , Análise Multivariada , Razão de Chances , Valor Preditivo dos Testes , Prognóstico , Estudos Prospectivos , Fatores de Proteção , Fatores de Risco , Regulação para Cima
19.
Naturwissenschaften ; 103(3-4): 29, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26940061

RESUMO

The plant secondary metabolite 6-methoxybenzoxazolinone (6-MBOA) can stimulate and enhance animal reproduction. This compound has been successfully detected in Leymus chinensis, which is the main diet of Brandt's voles. The aim of this study was to investigate the effect of different 6-MBOA doses on the reproductive physiology of male Brandt's voles under a short photoperiod. The results showed that 6-MBOA administration increased relative testis weight, regardless of the dose, but it had little effect on the body mass. Low and middle doses of 6-MBOA increased the concentrations of luteinizing hormone and testosterone in the serum and the mRNA levels of StAR and CYP11a1 in the testes. However, 6-MBOA did not cause any significant increase in the mRNA levels of KiSS-1, GPR54, and GnRH compared to those in the control group. The mRNA level of KiSS-1 in the arcuate nucleus (ARC) was higher than that in the anteroventral periventricular nucleus (AVPV). Collectively, our results demonstrated that the number of KiSS-1-expressing neurons located in the ARC was the highest, and that 6-MBOA, which might modulate the reproductive activity along the hypothalamic-pituitary-gonadal axis, had a dose-dependent stimulatory effect on the reproductive activity of Brandt's voles under a short photoperiod. Our study provided insights into the mechanism of 6-MBOA action and the factors influencing the onset of reproduction in Brandt's voles.


Assuntos
Arvicolinae/fisiologia , Benzoxazóis/farmacologia , Fotoperíodo , Reprodução/efeitos dos fármacos , Animais , Regulação da Expressão Gênica/efeitos dos fármacos , Hormônio Luteinizante/sangue , Masculino , Neurônios/efeitos dos fármacos , Testículo/efeitos dos fármacos , Testosterona/sangue
20.
Guang Pu Xue Yu Guang Pu Fen Xi ; 36(7): 2318-24, 2016 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30036021

RESUMO

This article focused on the assessment of the potential of Raman spectroscopy for the determination of structural changes in black-bean protein isolate (BBPI) dispersions with low-frequency (20 kHz) ultrasonication applied at various powers (150, 300 or 450 W) and for different durations (12 or 24 min). It also reported on differential scanning calorimetry analyses. A decrease in TD at low- and medium-power ultrasonication confirmed these ultrasonication treatment disrupted internal hydrophobic interactions of protein molecules and broke up unstable aggregates to smaller soluble protein aggregates, while an increase in TD at high-power was attributed to repolymerization of aggregates. Raman spectroscopy analysis revealed a decrease in the α-helix proportion and an increase in ß-sheets after ultrasonic treatment except Sample E (300 W, 24 min). Transformation of aggregation results in a reconstruction in secondary structure of BBPI, especially in ß-sheet structure. Ultrasonic-treatment induced a decrease in the normalized intensity of the Raman band near 760 cm-1 which indicated that Tryptophan residues tended to expose and also indicated protein partially unfolding. No significant difference was found in Tyr doublet ratios between unheated and ultrasound-treated BBPI indicated that ultrasound did not change the microenvironment around tyrosyl residues. While the intensity of 1 450 cm-1 band increased with increasing ultrasonic intensity and treatment time, and then decreased with further increase in power and treatment time. In general, the formation of aggregation transferred g-g-t conformation to t-g-t conformation. Though some mechanism of aggregation-repolymerization of BBPI remains to be clearly defined, Raman spectroscopy provide a feasible tool to study the structural changes of BBPI prepared under different ultrasonic conditions, give a new perspective to elucidation of protein structure.

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