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2.
Hum Reprod ; 2022 May 08.
Artigo em Inglês | MEDLINE | ID: mdl-35526155

RESUMO

STUDY QUESTION: Do variants in helicase for meiosis 1 (HFM1) account for male infertility in humans? SUMMARY ANSWER: Biallelic variants in HFM1 cause human male infertility owing to non-obstructive azoospermia (NOA) with impaired crossover formation and meiotic metaphase I (MMI) arrest. WHAT IS KNOWN ALREADY: HFM1 encodes an evolutionarily conserved DNA helicase that is essential for crossover formation and completion of meiosis. The null mutants of Hfm1 or its ortholog in multiple organisms displayed spermatogenic arrest at the MMI owing to deficiencies in synapsis and severe defects in crossover formation. Although HFM1 variants were found in infertile men with azoospermia or oligozoospermia, the causal relationship has not yet been established with functional evidence. STUDY DESIGN, SIZE, DURATION: A Pakistani family, having two infertile brothers born to consanguineous parents, and three unrelated Chinese men diagnosed with NOA were recruited for pathogenic variants screening. PARTICIPANTS/MATERIALS, SETTING, METHODS: All the patients were diagnosed with idiopathic NOA and, for the Chinese patients, meiotic defects were confirmed by histological analyses and/or immunofluorescence staining on testicular sections. Exome sequencing and subsequent bioinformatic analyses were performed to screen for candidate pathogenic variants. The pathogenicity of identified variants was assessed and studied in vivo in mice carrying the equivalent mutations. MAIN RESULTS AND THE ROLE OF CHANCE: Six variants (homozygous or compound heterozygous) in HFM1 were identified in the three Chinese patients with NOA and two brothers with NOA from the Pakistani family. Testicular histological analysis revealed that spermatogenesis is arrested at MMI in patients carrying the variants. Mice modeling the HFM1 variants identified in patients recapitulated the meiotic defects of patients, confirming the pathogenicity of the identified variants. These Hfm1 variants led to various reductions of HFM1 foci on chromosome axes and resulted in varying degrees of synapsis and crossover formation defects in the mutant male mice. In addition, Hfm1 mutant female mice displayed infertility or subfertility with oogenesis variously affected. LIMITATIONS, REASONS FOR CAUTION: A limitation of the current study is the small sample size. Owing to the unavailability of fresh testicular samples, the defects of synapsis and crossover formation could not be detected in spermatocytes of patients. Owing to the unavailability of antibodies, we could not quantify the impact of these variants on HFM1 protein levels. WIDER IMPLICATIONS OF THE FINDINGS: Our findings provide direct clinical and in vivo functional evidence that HFM1 variants cause male infertility in humans and also suggest that HFM1 may regulate meiotic crossover formation in a dose-dependent manner. Noticeably, our findings from mouse models showed that HFM1 variants could impair spermatogenesis and oogenesis with a varying degree of severity and might also be compatible with the production of a few spermatozoa in men and subfertility in women, extending the phenotypic spectrum of patients with HFM1 variants. STUDY FUNDING/COMPETING INTEREST(S): This work was supported by the National Natural Science Foundation of China (31890780, 32070850, 32061143006, 32000587 and 31900398) and the Fundamental Research Funds for the Central Universities (YD2070002007 and YD2070002012). The authors declare no potential conflicts of interest. TRIAL REGISTRATION NUMBER: N/A.

3.
World J Surg Oncol ; 20(1): 145, 2022 May 06.
Artigo em Inglês | MEDLINE | ID: mdl-35524309

RESUMO

BACKGROUND: With the development of laparoscopic techniques and the broad clinical application of various anastomotic types, anal-preserving low anterior rectal resection and ultra-low anterior rectal resection have been popularized. Some patients with rectal cancer have retained their anus and improved their quality of life. Nevertheless, the incidence of postoperative anastomotic stenosis remains high, and anastomotic occlusion is even rarer. CASE PRESENTATION: We report a case of anastomotic occlusion in a patient with rectal cancer, which occurred after undergoing laparoscopic low anterior rectal resection + prophylactic terminal ileal fistulation at our department. Under endoscopy, we used a small guidewire to break through the occluded anastomosis, thereby finding the lacuna. After endoscopic balloon dilation, digital anal dilatation, and continuous dilator-assisted dilation, the desired efficacy was achieved, ultimately recovering ileal stoma. Postoperative follow-up condition was generally acceptable, without symptoms like abdominal pain, bloating, or difficulty in defecation. CONCLUSION: Numerous factors cause postoperative anastomotic stenosis in patients with rectal cancer. Complete occlusion of anastomosis occurs relatively rare in clinical practice, and is challenging to treat. This case was our first attempt to remove the anastomotic occlusion successfully, which avoided re-operation or pain from the permanent fistula.


Assuntos
Laparoscopia , Neoplasias Retais , Anastomose Cirúrgica/efeitos adversos , Fístula Anastomótica/cirurgia , Constrição Patológica/etiologia , Constrição Patológica/cirurgia , Humanos , Laparoscopia/efeitos adversos , Laparoscopia/métodos , Complicações Pós-Operatórias/epidemiologia , Qualidade de Vida , Neoplasias Retais/complicações , Neoplasias Retais/cirurgia , Estudos Retrospectivos
4.
Chemistry ; 2022 May 12.
Artigo em Inglês | MEDLINE | ID: mdl-35560734

RESUMO

Ultrathin two-dimensional metal-organic framework nanosheets have emerged as a promising kind of heterogeneous catalysts. Herein, we report a series of 2D porphyrinic metal-organic framework nanosheets (X-PMOF, X = F, Cl, Br), which was prepared from the self-assembly of a halogen-based porphyrin ligand X-TCPP (X-TCPP = 5-(4-halogenatedphenyl)-10,15,20-tris(4-carboxyphenyl)-porphyrin) and ZrCl 4 in the presence of trifluoroacetic acid as the modulating reagent. The framework of X-PMOF possessed the ftw topology as in MOF-525. The lamellar X-PMOF nanosheets with the thickness of down to 4.5 nm were assembled and aggregated into flower-like morphology. With the introduction of iridium(III) atoms into the porphyrin rings, the resultant X-PMOF(Ir) nanosheets were prepared via the similar method. Catalytic results show that Br-PMOF(Ir) nanosheets were highly efficient for CO 2 reduction and aminolysis, giving rise to formamides in high yields under room temperature and atmospheric pressure, and can be recycled and reused for 3 runs. The total turnover number of Br-PMOF(Ir) after 3 runs was 1644 based on Ir. Mechanistic studies disclose that the high efficiency of Br-PMOF(Ir) nanosheets was ascribed to three factors, including the superior activation capability of iridium(III) porphyrin for Si-H bonds, more active sites on the external surfaces of Br-PMOF(Ir) nanosheets, and the defects caused by unsymmetrical porphyrin ligand that increased the framework's affinity towards CO 2 .

5.
Zhonghua Wei Zhong Bing Ji Jiu Yi Xue ; 34(3): 250-254, 2022 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-35574740

RESUMO

OBJECTIVE: To investigate the effect of intensive care unit (ICU) admission model on acute kidney injury (AKI) development and the prognosis in patients with sepsis. METHODS: Patients with sepsis admitted to the ICU of Xinjiang Uygur Autonomous Region People's Hospital from January 2019 to July 2020 were retrospectively analyzed. According to the ICU admission model, the patients were divided into emergency group (first admission or emergency transfer from relevant surgical departments) and delayed group (transferred from the general ward due to disease evolution). Patients were divided into AKI group and non-AKI group according to whether AKI was accompanied. The gender, age, underlying diseases, surgical history, heart rate, laboratory test indicators, acute physiology and chronic health evaluation II (APACHE II), sequential organ failure assessment (SOFA), organ failure and acute complications were collected. The incidence of AKI, 28-day mortality and length of ICU stay were recorded. Univariate and multivariate Logistic regression was used to analyze the risk factors of AKI in patients with sepsis. RESULTS: A total of 185 patients with sepsis were enrolled, including 96 cases in the emergency group and 89 cases in the delayed group. 119 cases of AKI occurred while 66 cases without AKI development. The incidence of AKI within 7 days and the 28-day mortality of patients in the delayed group were significantly higher than those in the emergency group [AKI incidence rate: 77.53% (69/89) vs. 52.08% (50/96), 28-day mortality: 24.72% (22/89) vs. 10.42% (10/96), both P < 0.05], and the length of ICU stay was significantly longer than that of the emergency group (days: 18.70±7.29 vs. 14.56±4.75, P < 0.05). Univariate Logistic analysis showed that there were significant differences in age, diabetes, hypertension, organ failure, heart failure, APACHE II score, SOFA score, white blood cell count (WBC), absolute neutrophil value, platelet count (PLT), blood lactate, total bilirubin, and ICU transferred from general wards between AKI group and non-AKI group. Multivariate Logistic regression analysis showed that transfer from general ward to ICU due to disease evolution was an independent risk factor for AKI in ICU sepsis patients [odds ratio (OR) = 5.165, 95% confidence interval (95%CI) was 3.911-6.823, P < 0.001]. CONCLUSIONS: Septic patients transferred from general ward to ICU due to disease evolution are more likely to develop AKI, and also had a higher mortality and longer ICU stay. It may be an independent risk factor for AKI complicated by patients with sepsis in ICU.

6.
Oncoscience ; 9: 27-29, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35548777

RESUMO

CRISPR/Cas9-based tools are anticipated to transform the gene therapy field by facilitating the correction of disease-causing mutations. However, CRISPR/Cas9 generates DNA damage, which triggers a DNA damage response centered around the tumor-suppressor p53. In this research perspective, we discuss implications of this and describe a CRISPR-p53 interactome with cancer-related genes that, if mutated, can give cells a selective advantage following exposure to CRISPR/Cas9. We propose that the genes in the CRISPR-p53 interactome should be monitored in the clinical setting and describe that transient p53 inhibition could be used to limit the enrichment of cells with such mutations.

7.
Comput Intell Neurosci ; 2022: 2480801, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35371220

RESUMO

In this study, 9 maize inbred lines and 36 combinations were used as materials to analyze the combining ability of plant height, ear height, kernel depth, grain water content, tassel branches, stem diameter, and 100 kernels weight so as to screen excellent inbred lines and maize combinations with suitable mechanical harvest characters, which would provide a theoretical basis for breeding new maize varieties suitable for mechanical harvest. The results showed that JK2023, JK2368, and JK2018 were inbred lines whose comprehensive characters met the machine harvest standard and performed well. Besides, the combinations that meet the machine harvest standard and perform well were JK2023 × JK2197, JK2023 × JK2368, JK2023 × JK2005, JK2197 × JK2005, JK2197 × JK2368, and JK2368 × JK2005.


Assuntos
Zea mays , Zea mays/genética
8.
J Clin Lipidol ; 2022 Mar 17.
Artigo em Inglês | MEDLINE | ID: mdl-35379578

RESUMO

BACKGROUND: There is a lack of large-scale data on the clinical and genotype characteristics of homozygous familial hypercholesterolemia (HoFH) patients in Asia. OBJECTIVE: To define the characteristics of phenotypic and genetic HoFH probands from mainland China. METHODS: We collected data from patients with suspected HoFH from ten clinical hospitals across mainland China from 2003 to 2019. Clinical data and DNA testing were obtained in all patients. The Kaplan-Meier method was used to generate survival curves, and the groups were compared with the log-rank test. RESULTS: A total of 108 unrelated probands with suspected HoFH (mean age 14.9 years) were included. The three most common variants were W483X (c.1448 G>A), A627T (c.1879 G>A), H583Y (c.1747 C>T). The majority (64.8%) were compound heterozygotes (n = 70), 23 (21.3%) were true HoFH patients. True HoFH showed higher LDL-C levels compared to compound HoFH (16.8±3.6 mmol/L vs. 15.0±3.1 mmol/L, P = 0.022). During follow-up, only 21.2% patients exhibited an LDL-C reduction of more than 50%. Kaplan-Meier analysis showed that the true HoFH probands had significantly worse survival rates compared to other genotype probands (13-year survival; 20.3% vs. 76.7%, respectively; P = 0.016). In addition, true HoFH shows that 2.8-fold (P = 0.022) increase any death and 3.0-fold (P = 0.023) increase cardiovascular death risk in relative to other FH. CONCLUSIONS: This report shows that HoFH has devastating consequences, and that patients are often only diagnosed after they have been exposed to severely elevated LDL-C for years. Systematic screening and early intensive treatment are an absolute requirement for these young individuals with HoFH.

9.
Cell Rep ; 39(1): 110609, 2022 04 05.
Artigo em Inglês | MEDLINE | ID: mdl-35385733

RESUMO

Tumor-associated macrophages (TAMs) are a major cellular component in the tumor microenvironment (TME). However, the relationship between the phenotype and metabolic pattern of TAMs remains poorly understood. We performed single-cell transcriptome profiling on hepatic TAMs from mice bearing liver metastatic tumors. We find that TAMs manifest high heterogeneity at the levels of transcription, development, metabolism, and function. Integrative analyses and validation experiments indicate that increased purine metabolism is a feature of TAMs with pro-tumor and terminal differentiation phenotypes. Like mouse TAMs, human TAMs are highly heterogeneous. Human TAMs with increased purine metabolism exhibit a pro-tumor phenotype and correlate with poor therapeutic efficacy to immune checkpoint blockade. Altogether, our work demonstrates that TAMs are developmentally, metabolically, and functionally heterogeneous and purine metabolism may be a key metabolic feature of a pro-tumor macrophage population.


Assuntos
Neoplasias Hepáticas , Microambiente Tumoral , Animais , Perfilação da Expressão Gênica , Neoplasias Hepáticas/patologia , Macrófagos/metabolismo , Camundongos , Macrófagos Associados a Tumor
10.
Oncol Rep ; 47(6)2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35417034

RESUMO

Subsequently to the publication of the above article, an interested reader drew to the authors' attention that the data panel for the MDA­MB­231/migration/NC experiment in Fig. 2B on p. 1428 was strikingly similar to the data shown for the MDA­MB­231/invasion/Blank experiment in Fig. 2C, such that these data appeared to have been derived from the same original source. The authors have referred back to their original data, and realize that the data panel was selected incorrectly for Fig. 2B. The corrected version of Fig. 2, showing the correct data for the MDA­MB­231/migration/NC experiment in Fig. 2B, is shown on the next page. The authors regret the error that was made during the preparation of this figure, and can confirm that the error in the assembly of this figure did not adversely affect the conclusions reported in the study. The authors are grateful to the Editor of Oncology Reports for allowing them the opportunity to publish a Corrigendum, and all the authors agree to this Corrigendum. Furthermore, they apologize to the readership for any inconvenience caused. [the original article was published in Oncology Reports 35: 1425­1432, 2016; DOI: 10.3892/or.2015.4502].

11.
Int J Biol Macromol ; 207: 927-940, 2022 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-35364194

RESUMO

Up to now, unbalanced mechanical properties and poor heat resistance have become two major problems of polylactic acid (PLA). In this study, the coupling between Cellulose nanocrystal (CNC) and strong shearing field formed a unique hierarchical structure. Compared with pure PLA, the tensile strength of DPIM PLA/CNC increased from 57.9 MPa to 79.6 MPa without sacrificing the toughness of PLA, and the vicat softening temperature of DPIM PLA/CNC increased from 60 °C to 155 °C. The microstructure of PLA/CNC composites was analyzed by SEM, SAXS and WAXD, and it was found that the coupling effect of CNC and strong shear flow field could significantly change the crystallization behavior of PLA. CNC could increase PLA shish length from 251 nm to 889 nm under the action of shear field. At the same time, due to this coupling effect, more PLA shish-kebab structures were induced at the interface. This special hierarchical structure composed of CNC and PLA Shish-Kebab is of great significance and can provide important guidance for achieving the balance of strength and toughness of polymer materials.


Assuntos
Celulose , Nanopartículas , Celulose/química , Nanopartículas/química , Poliésteres/química , Espalhamento a Baixo Ângulo , Difração de Raios X
12.
Front Oncol ; 12: 782030, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35480094

RESUMO

Objective: To examine the effect of primary recurrence patterns on the prognosis of squamous cervical cancer after initial treatment. Methods: Primary recurrence patterns and prognostic factors were examined in stage IB-IIA cervical cancer patients after initial treatment. Recurrence site (locoregional recurrence and distant metastasis or in-field and out-field recurrence for patients receiving adjuvant radiotherapy) and subtype (nodal and organ recurrence) were examined. Clinicopathological characteristics and survival rates were evaluated to generate a prognostic nomogram. Results: A total of 472 patients were included. The median follow-up period, 5-year overall (OS) rate, and median OS were 59.1 months, 33.7%, and 24.0 months, respectively. Overall, 38.8% and 61.2% of the patients had locoregional recurrence and distant metastasis, respectively, and survival rates were comparable in these groups. Patients with nodal recurrence had better OS than those with organ recurrence (38.3% vs 30.7%, respectively; P = 0.001). Patients not receiving adjuvant radiotherapy had increased risk of pelvic recurrence [odds ratio (OR) = 0.148; 95% confidence interval[(CI): 0.075-0.291, P = 0.000]. Positive lymph-vascular space invasion (OR= 1.928; 95% CI: 1.151-3.229, P = 0.013) and no chemotherapy (OR = 0.521; 95% CI: 0.317-0.733, P = 0.040) increased the risk of distant metastasis. Positive lymph node status after initial treatment were associated with nodal recurrence (OR = 3.729; 95% CI: 1.838-7.563, P = 0.000), while elevated preoperative squamous cell carcinoma antigen (SCC-Ag) levels were associated with organ recurrence (OR = 1.642; 95% CI: 1.325-2.265, P = 0.002). Recurrence subtype, therapy for relapse, the International Federation of Gynecology and Obstetrics stage, adjuvant radiotherapy, preoperative SCC-Ag levels, and risk subgroup were independently associated with OS. Conclusions: Primary recurrence patterns were associated with specific clinicopathological characteristics of cervical cancer. Recurrent cervical cancer prognosis was mainly affected by recurrence location and subtype.

13.
J Mater Chem B ; 2022 Apr 04.
Artigo em Inglês | MEDLINE | ID: mdl-35377383

RESUMO

The development of an elution-free solid-phase extraction (SPE) process is of special interest in sample pretreatment. Due to the phase-change merits at relatively low temperatures and easy dissolution in n-hexane, wax spheres show great potential in this field. However, the conventional wax spheres possess a low affinity towards the target analytes when they are used as SPE adsorbents. In this study, using octadecanoic acid as the functional monomer and wax as the matrix, molecularly imprinted wax (MIW) spheres were successfully prepared. The obtained MIW spheres displayed remarkable molecular recognition ability and high selectivity towards the template. Interestingly, the as-synthesized molecularly imprinted wax (MIW) could be dissolved in n-hexane or melted by heating for subsequent fluorescence and mass spectrum analysis without the target elution process. Moreover, the melted MIW exhibited high repeatability, sensitivity and specificity for solid-state fluorescence detection. We believe that the imprinting method presented in this study will open a new window in analytical chemistry.

15.
Asian J Surg ; 2022 Mar 04.
Artigo em Inglês | MEDLINE | ID: mdl-35256258
16.
Chem Commun (Camb) ; 58(29): 4596-4598, 2022 Apr 07.
Artigo em Inglês | MEDLINE | ID: mdl-35229100

RESUMO

Luminescent crystals with high efficiency have huge potential in applications for white light-emitting diodes (LEDs). Herein, organic-inorganic hybrid crystals doped with Mn4+, [N(CH3)4]2XF6:Mn4+ (X = Si, Ge, and Ti), were grown under mild conditions. Their crystal structural evolution was confirmed by single-crystal X-ray diffraction at different temperatures. These crystals exhibit intense red emission with a high external quantum efficiency (73.0% for [N(CH3)4]2TiF6:Mn4+) and good thermal stability. The warm white LEDs were fabricated by combining these red-emitting phosphors with a YAG:Ce3+ ceramic chip. As-grown crystals can significantly optimize the performances of white LEDs (colour rendering index up to 95). Hence, this work provides a new strategy to explore Mn4+-activated organic-inorganic hybrid materials for white LEDs.

17.
Langmuir ; 38(12): 3868-3875, 2022 03 29.
Artigo em Inglês | MEDLINE | ID: mdl-35298179

RESUMO

The development of isothermal nucleic acid amplification techniques has great significance for highly sensitive biosensing in modern biology and biomedicine. A facile and robust exponential rolling circle amplification (RCA) strategy is proposed based on primer-remodeling amplification jointly via a repair enzyme and polymerase, and uracil-DNA glycosylase (UDG) is selected as a model analyte. Two kinds of complexes, complex I and complex II, are preprepared by hybridizing a circular template (CT) with a uracil-containing hairpin probe and tetrahydrofuran abasic site mimic (AP site)-embedded fluorescence-quenched probe (AFP), respectively. The target UDG specifically binds to complex I, resulting in the generation of an AP site, followed by cleavage via endonuclease IV (Endo IV) and the successive trimming of unmatched 3' terminus via phi29 DNA polymerase, thus producing a useable primer-CT complex that actuates the primary RCA. Then, numerous complex II anneal with the first-generation RCA product (RP), generating a complex II-RP assembly containing AP sites within the DNA duplex. With the aid of Endo IV and phi29, AFP, as a pre-primer in complex II, is converted into a mature primer to initiate additional rounds of RCA. So, countless AFPs are cleaved, releasing remarkably strong fluorescent signals. The biosensor is demonstrated to enable rapid and accurate detection of the UDG activity with an improved detection limit as low as 4.7 × 10-5 U·mL-1. Moreover, this biosensor is successfully applied for UDG inhibitor screening and complicated biological samples analysis. Compared to the previous exponential RCA methods, our proposed strategy offers additional advantages, including excellent stability, optional design of CT, and simplified operating steps. Therefore, this proposed strategy may create a useful and practical platform for ultrasensitive detection of low levels of analytes in clinical diagnosis and fundamental biomedicine research.


Assuntos
Técnicas Biossensoriais , Uracila-DNA Glicosidase , Técnicas Biossensoriais/métodos , Reparo do DNA , Corantes Fluorescentes , Limite de Detecção , Técnicas de Amplificação de Ácido Nucleico/métodos , Uracila-DNA Glicosidase/análise , Uracila-DNA Glicosidase/metabolismo , alfa-Fetoproteínas
18.
Mol Cancer Res ; 2022 Mar 29.
Artigo em Inglês | MEDLINE | ID: mdl-35348737

RESUMO

Targeting the DNA damage response in combination with radiation enhances type I interferon (T1IFN)-driven innate immune signaling. It is not understood, however, whether DNA-dependent protein kinase (DNA-PK), the kinase critical for repairing the majority of radiation-induced DNA double-strand breaks in cancer cells, is immunomodulatory. We show that combining radiation with DNA-PK inhibition increases cytosolic double-stranded DNA and tumoral T1IFN signaling in a cGAS- and STING-independent, but an RNA POL III, RIG-I, and MAVS-dependent manner. Although DNA-PK inhibition and radiation also promote programmed death-ligand 1 (PD-L1) expression, the use of anti-PD-L1 in combination with radiation and DNA-PK inhibitor potentiates antitumor immunity in pancreatic cancer models. Our findings demonstrate a novel mechanism for the antitumoral immune effects of DNA-PK inhibitor and radiation that leads to increased sensitivity to anti-PD-L1 in poorly immunogenic pancreatic cancers. Implications: Our work nominates a novel therapeutic strategy as well as biomarkers of treatment resistance pertinent for future clinical trials combining M3814, radiation and αPD-L1 antibody in patients with pancreatic cancer.

19.
Oncologist ; 27(1): e53-e63, 2022 02 03.
Artigo em Inglês | MEDLINE | ID: mdl-35305100

RESUMO

OBJECTIVE: The objective of this study was to retrospectively explore the clinical implications of simultaneous intensity-modulated radiotherapy (IMRT) boost to the tumor bed in cervical cancer with full-thickness stromal invasion (FTSI). PATIENTS AND METHODS: Patients diagnosed with the International Federation of Gynecology and Obstetrics (FIGO) 2009 stage IB and IIA cervical cancer with confirmed FTSI were included. Patients received pelvic IMRT from a dose of 50.4 Gy in 28 fractions with (or without) a simultaneous integrated boost (SIB) to 58.8 Gy in 28 fractions for the tumor bed. The progression-free survival (PFS), overall survival (OS), and pelvic-PFS (p-PFS) were analyzed using the Kaplan-Meier method, and independent prognostic factors were explored by Cox regression analyses. RESULTS: Patients without a tumor bed boost had a poor prognosis. The 5-year OS was 81.3% versus 58.3% and the 5-year PFS rates were 75.0% versus 57.6% (boost vs non-boost). The FIGO stage, pathology, adjuvant chemotherapy, and tumor bed boost were independent factors affecting both the 5-year OS and PFS. Subgroup analysis showed that the SIB group had a higher 5-year OS, PFS, and p-PFS for different stages, lymph node status, and risk groups than the non-SIB group. Recurrence occurred in 268 of 910 (29.5%) patients without SIB and 49 of 293 (16.7%) with SIB. Among patients with recurrence, 113 of 282 (40.1%) in the non-boost group compared with 14 of 51 (23.0%) patients in the boost group had a pelvic recurrence. Tumor bed boost resulted in an increase in the mean radiation dose to the intestine, rectum, and bladder, although there were no differences in the rates of acute and late toxicities between the 2 groups. CONCLUSION: Tumor bed boost by external beam radiotherapy (EBRT) is an effective and safe method for patients with FTSI and risk factors. Compared with the standard prophylactic radiation, tumor bed boost by EBRT was not associated with increased acute and late toxicities.


Assuntos
Radioterapia de Intensidade Modulada , Neoplasias do Colo do Útero , Quimioterapia Adjuvante , Feminino , Humanos , Dosagem Radioterapêutica , Planejamento da Radioterapia Assistida por Computador/métodos , Radioterapia de Intensidade Modulada/efeitos adversos , Estudos Retrospectivos , Neoplasias do Colo do Útero/patologia
20.
J Am Chem Soc ; 144(10): 4305-4309, 2022 03 16.
Artigo em Inglês | MEDLINE | ID: mdl-35254816

RESUMO

Molecular photocatalysts designed with earth-abundant elements are rare and challenging in artificial photosynthesis study. Herein, we report a multimetallic Fe2Na3 purpurin (1) complex for the reduction of CO2 in DMF under visible-light irradiation. The photocatalytic system achieves 91% selectivity and 2625 ± 334 turnovers of CO in 120 h, which is among the highest reported for a noble-metal-free catalyst without an additional photosensitizer. UV-vis and electrochemical studies suggest that the mechanism involves subsequent reductions and protonations of 1 to generate [FeII2Na3((H)2PP)6]5- and [FeIII2Na3((H)2PP)6]3- as the active photocatalysts in CO2 reduction.


Assuntos
Dióxido de Carbono , Ferro , Antraquinonas , Dióxido de Carbono/química , Catálise , Corantes/química , Íons , Ferro/química
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