Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 18 de 18
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Inflamm Res ; 2022 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-36329130

RESUMO

BACKGROUND: Rosacea, a chronic inflammatory disorder of the facial skin, is effectively treated by intense pulsed light (IPL). OBJECTIVE: To explore the potential molecular mechanism underlying the photobiomodulation effect of IPL for rosacea treatment. METHODS: Skin samples from patients with rosacea were subjected to histological and immunohistological staining. Ten patients were followed up after IPL treatment using the VISIA® skin analysis system, and the severity was assessed. In vivo, skin changes in mice with rosacea-like inflammation induced by intradermal injection of 320 µM LL-37 with or without IPL treatment were evaluated using L*a*b colorimetry as well as histological and immunological staining. In vitro, LL-37-stimulated mast cells (MCs) with or without IPL treatment were evaluated for protein expression of matrix metalloproteinase (MMP)-9, kallikrein-related peptidase 5 (KLK5), and cathelicidin using western blotting and qRT-PCR. RESULTS: Profound infiltration of inflammatory cells and evident MC degranulation were found in rosacea skin lesions. The expression of rosacea-related biomarkers and inflammatory cytokines was higher in lesional areas than in non-lesional areas, as demonstrated via immunochemical staining. In all patients, rosacea severity reduced after IPL therapy. In vivo, IPL alleviated inflammation in mice with rosacea-like inflammation, as demonstrated by the significantly decreased MMP-9, KLK5, and cathelicidin expression and reduced percentage of degranulating MCs. In vitro, IPL decreased MMP-9, KLK5, and cathelicidin expression in P815 cells, reducing the release of inflammatory cytokines and inhibiting rosacea-like inflammatory reactions. CONCLUSION: The photobiomodulation effect of IPL for rosacea treatment may inhibit MC degranulation and alleviate inflammatory reactions.

2.
Skin Res Technol ; 2022 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-36426837

RESUMO

BACKGROUND: Facial erythema, a prominent characteristic of rosacea, causes concern to both the patient and doctor. In clinical practice, commonly used erythema severity subjective assessment tools lack objectivity and are less comprehensive. Even with images taken by the VISIA® system, diffused erythema is difficult to segment and evaluate fully due to the automatic threshold segmentation method. This study aimed to explore a more objective and scientific erythema quantification tool with the aid of the ImageJ software analysis of the red area images taken by the VISIA® system. MATERIALS AND METHODS: Patients with rosacea were enrolled and assessed for the clinical severity of their illness using various stools-the standard grading systems (SGS) for rosacea, investigator's global assessment (IGA), and clinician's erythema assessment (CEA). Facial images in the red area mode of the VISIA® system were further analyzed by the ImageJ for the relative intensity of redness and percentage of erythema area; the correlation with the scores of the subjective grading systems was evaluated. RESULTS: This study included 201 patients (195 females and 6 males). The relative intensity of redness was positively correlated to the SGS, IGA, and CEA scores (0.688, 0.725, and 0.718, respectively) (p < 0.001). The percentage of erythema area was positively correlated to the SGS, IGA, and CEA scores (0.615, 0.666, and 0.656, respectively) (p < 0.001). CONCLUSION: We demonstrated a more objective and precise method of assessing the severity of facial erythema rosacea, which could comprehensively assess the severity by both the area and intensity of facial erythema.

5.
J Chromatogr A ; 1671: 462990, 2022 May 24.
Artigo em Inglês | MEDLINE | ID: mdl-35390735

RESUMO

The detection of Chemical Weapon Convention (CWC)-related amine compounds including the precursors or degradation products of V-type organophosphorus nerve agent, nitrogen mustard and 3-quinuclidinyl benzilate is an important aspect for verifying their intact chemical warfare agents. This work focuses on the development of a novel formulation for the simultaneous solvent extraction of eleven CWC-related amine compounds, from the four-type soil matrices including environmental standard soil, sand, clay, and loam. Extracts were well separated on the hydrophilic interaction liquid chromatography (HILIC) and then detected by MS/MS multiple reaction monitoring mode. The type and component of solvent mixtures were optimized to cover a wide range of polarity over all eleven amine compounds with high extraction efficiencies. Extraction parameters, such as the proportion of methanol, water and NH4OH, the times and the period of extraction, and volumes of extraction solution were optimized. The results indicated that a mixed solvent of methanol/water (44:53, v/v) in 3.0% NH4OH was the optimal formulation for extraction of all 11 analytes with high mean extraction recoveries (64.4-96.1%). Specificity and sensitivity were well improved by the good separation of 11 analytes from four-type soil matrices using these optimized HILIC parameters. This method was fully validated for each analyte in four soil matrices. The linear range of 11 analytes was 0.50/0.75-500 ng·g-1 with correlation coefficient (R2) ≥0.990, and intra/inter-day accuracies were 70.3-125% with relative standard deviation (RSD) ≤19.3%. Limit of detection (LOD) of 11 analytes ranged from 0.01 to 0.5 ng·g-1, which was far lower than those reported in previous studies. The built method accomplishes simultaneously quantitative and trace measurement of all eleven CWC-related amine compounds within a single solvent extraction and detection. It only takes a small amount of soil samples and possesses the highest sensitivity over all previous methods. This study provides an optional recommended operating procedure for determination of CWC-related amine compounds in four typical types of complex soils during chemical weapons verification.


Assuntos
Agentes Neurotóxicos , Espectrometria de Massas em Tandem , Aminas , Cromatografia Líquida de Alta Pressão , Cromatografia Líquida , Interações Hidrofóbicas e Hidrofílicas , Metanol , Compostos Organofosforados , Solo/química , Extração em Fase Sólida , Solventes , Água
6.
Anal Bioanal Chem ; 414(8): 2713-2724, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-35083511

RESUMO

Organophosphorus nerve agents (OPNAs) covalently bind to tyrosine 411 of human serum albumin (HSA) and the formed adducts are stable biomarkers of OPNA exposure. The detection of these adducts has been limited to mass spectrometry techniques combined with protein digestion. Here, we developed indirect competitive ELISA (icELISA) methods to verify OPNA exposure by the detection of OPNA-phosphonylated adducts at tyrosine 411 residue (OPNA-HSA adducts), in which monoclonal antibodies (mAbs) against phosphonylation sites at tyrosine 411 were introduced. The two mAbs were prepared by the fourth generation of rabbit mAb technology using the phosphonylated peptides of LVRY(GD or VX)TKKVPQC as the haptens. These mAbs were screened using our developed competitive ELISA method and then selected based on their individual affinity and selectivity. As a result, we obtained two mAbs that recognized the HSA Tyr 411 adduct of GD (mAb-5G2) or VX (mAb-12B9), respectively. They shared the highest affinity exhibiting a Kd value of about 10-6 mol/L of the OPNA exposure concentration. They also had remarkable selectivity, which could especially recognize their individual OPNA-HSA adducts in a native state but did not recognize other OPNA-HSAs and unadducted HSAs. Especially for mAb-12B9, it could clearly distinguish VX-HSA and GB-HSA between which there was only one alkyl difference in their phosphonyl portion of the adducted sites. The two mAbs were then used to build the icELISA method for analysis of the serum samples exposed to OPNA. It was found that the detectable lowest GD- and VX-exposed concentrations in serum samples were respectively 1.0 × 10-6 mol/L and 10.0 × 10-6 mol/L. This study provides one novel approach and strategy for the retrospective detection of OPNA exposure, and the two mAbs have great potential to be extended for point-of-care testing of OPNA intoxication.


Assuntos
Soman , Animais , Anticorpos Monoclonais , Ensaio de Imunoadsorção Enzimática , Compostos Organotiofosforados , Coelhos , Estudos Retrospectivos
7.
Front Immunol ; 12: 609615, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34322115

RESUMO

Background: Rosacea, a chronic inflammatory skin disorder etiologically associated with immune cells and the antibacterial peptide cathelicidin LL-37, can be effectively treated by oral carvedilol administration. Objective: To investigate the molecular mechanisms underlying carvedilol efficacy in rosacea treatment. Methods: Skin samples of patients with rosacea were subjected to histopathological (hematoxylin and eosin) and immunohistochemical (CD68, Toll-like receptor 2 (TLR2), kallikrein 5, cathelicidin, TNF-α, and IL-1ß) evaluation. An in vivo murine rosacea-like inflammation model was established by LL-37 intradermal injection with or without carvedilol gavage-based pretreatment. Erythema proportion (Image J) and skin redness (L*a*b colorimetry) were quantified. Murine skin samples underwent pathological examination for inflammatory status and immunofluorescence staining. Murine skin and lipopolysaccharide-stimulated RAW 264.7 cells with or without carvedilol pretreatment were evaluated by quantitative reverse transcription-polymerase chain reaction and western blotting. Clinical facial images of patients were obtained using the VISIA skin analysis system before, 4, and 6 months following oral carvedilol administration. Results: Rosacea skin lesions exhibited more pronounced inflammatory cell infiltration than peripheral areas, with profound macrophage infiltration and inflammatory cytokines (TLR2, kallikrein 5, cathelicidin, TNF-α, and IL-1ß). In vivo, carvedilol alleviated inflammation in LL-37 mice, down-regulating TLR2, KLK5, and cathelicidin expression. In vitro, carvedilol decreased TLR2 expression in RAW 264.7 cells, further reducing KLK5 secretion and LL-37 expression and ultimately inhibiting rosacea-like inflammatory reactions. Clinical manifestations and facial redness obviously improved during 6-month follow-up with systemic carvedilol administration. Conclusion: Carvedilol is effective against rosacea, with inhibition of macrophage TLR2 expression as a novel anti-inflammatory mechanism.


Assuntos
Anti-Inflamatórios/uso terapêutico , Carvedilol/uso terapêutico , Macrófagos/efeitos dos fármacos , Rosácea/tratamento farmacológico , Pele/efeitos dos fármacos , Receptor 2 Toll-Like/antagonistas & inibidores , Animais , Peptídeos Catiônicos Antimicrobianos/metabolismo , Citocinas/genética , Citocinas/metabolismo , Modelos Animais de Doenças , Feminino , Humanos , Mediadores da Inflamação/metabolismo , Calicreínas/genética , Calicreínas/metabolismo , Macrófagos/imunologia , Macrófagos/metabolismo , Camundongos , Camundongos Endogâmicos BALB C , Células RAW 264.7 , Rosácea/imunologia , Rosácea/metabolismo , Rosácea/patologia , Pele/imunologia , Pele/metabolismo , Pele/patologia , Receptor 2 Toll-Like/genética , Receptor 2 Toll-Like/metabolismo , Resultado do Tratamento , Catelicidinas
8.
Medicine (Baltimore) ; 100(3): e23986, 2021 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-33545988

RESUMO

ABSTRACT: Rosacea is a facial chronic inflammatory skin disease with immune and vascular system dysfunction. Paeoniflorin (PF) is a traditional Chinese medicine with anti-inflammatory properties. However, its effects on rosacea remain unknown. Here, we investigated the mechanisms through which PF inhibits the macrophage-related rosacea-like inflammatory response. Immunohistochemical methods were used to detect differences in the inflammatory response and degree of macrophage infiltration in granulomatous rosacea lesions and their peripheral areas. Cell Counting Kit-8 was used to determine the cytotoxicity of PF towards RAW 264.7 cells. Reverse transcription-quantitative polymerase chain reaction and western blotting were used to measure the influence of PF on mRNA and protein expression levels of suppressor of cytokine signaling 3 (SOCS3), apoptosis signal-regulating kinase 1 (ASK1)-p38, Toll-like receptor 2, and cathelicidin antimicrobial peptide ( or LL37) in the lipopolysaccharide (LPS)-induced macrophage-related rosacea-like inflammatory response of RAW 264.7 cells. Inflammatory cell infiltration was more pronounced in granulomatous rosacea lesions than in peripheral areas. LL37 expression increased significantly, and the infiltration of a large number of CD68+ macrophages was observed in the lesions. PF promoted SOCS3 expression in RAW 264.7 cells and inhibited the LPS-induced increase in toll-like receptor 2 and LL37 expression through the ASK1-p38 cascade, thereby alleviating the macrophage-related rosacea-like inflammatory response. These changes could be abrogated by SOCS3 siRNA in vitro.In conclusion, the pathogenesis of rosacea involves abnormal macrophage infiltration within the lesions. PF inhibits the macrophage-related rosacea-like inflammatory response through the SOCS3-ASK1-p38 pathway, demonstrating its potential application as a novel drug for rosacea therapy.


Assuntos
Anti-Inflamatórios não Esteroides/farmacologia , Glucosídeos/farmacologia , MAP Quinase Quinase Quinase 5/metabolismo , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Monoterpenos/farmacologia , Rosácea/tratamento farmacológico , Proteína 3 Supressora da Sinalização de Citocinas/metabolismo , Animais , Técnicas de Cultura de Células , Humanos , Macrófagos/metabolismo , Camundongos , Células RAW 264.7 , Pele/citologia
9.
J Cosmet Dermatol ; 20(9): 2715-2722, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-33350031

RESUMO

BACKGROUND: Rosacea and cardiovascular diseases (CVD) are chronic inflammatory disorders. While CVD is the leading cause of mortality globally, increasing evidence indicates that CVD prevalence could be higher among patients with rosacea. AIMS: This review aimed to determine the association between the prevalence of CVD and rosacea. PATIENTS/METHODS: A systematic review of observational studies with controls available in MEDLINE, EMBASE, PubMed, Cochrane, and Web of Science databases was conducted. We performed a pooled meta-analysis using random-effects weighting. Overall, 11 studies met the inclusion criteria, which indicated increased odds for at least one risk factor of CVD, including diabetes, high blood pressure, or dyslipidemia. RESULTS: The pooled meta-analysis indicated an association of rosacea with higher odds of insulin resistance or diabetes (odds ratio [OR], 1.18; 95% confidence interval [CI], 0.97-1.45), high systolic blood pressure (OR, 1.96; 95% CI, 1.35-2.84), dyslipidemia (OR, 1.50; 95% CI, 1.19-1.88), and CVD (OR, 6.65; 95% CI, 2.80-15.76). No publication bias was detected. The effect of confounding factors due to overlapping symptoms and lack of individual-level data were limitations of this review. CONCLUSION: Patients with rosacea have a high risk of CVD. However, further studies are warranted to confirm the association between rosacea and CVD.


Assuntos
Doenças Cardiovasculares , Rosácea , Doenças Cardiovasculares/epidemiologia , Doenças Cardiovasculares/etiologia , Humanos , Prevalência , Fatores de Risco , Rosácea/epidemiologia
10.
Environ Pollut ; 267: 115639, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33254659

RESUMO

Vehicle emissions are a major contributor to air pollution in China. In this study, a high-resolution inventory of eight on-road vehicle-emitted pollutants in 53 cities within the North China Plain (NCP) was established for 152 sub-sources. Monthly emission factors were then simulated using the COPERT v5 model and their spatial distribution at 4 km × 4 km resolution was allocated based on the transportation network. In 2017, emissions of BC, CO, NH3, NMVOCs, NOx, PM10, PM2.5, and SO2 were 38.3, 2900, 21.8, 578, 2460, 113, 85.9, and 4.7 kt, respectively. These emissions and their sources differed between cities, mainly due to different vehicle populations, fleet compositions, emission share rates of different vehicle types, and emission standards in each city. Small-medium petrol passenger cars and both 20-26 t and 40-50 t heavy-duty diesel trucks of China 3 and 4 emissions standards were the main contributors for all pollutants. Higher cold-start emission factors caused higher emissions of CO, NMVOCs, NOx, and PM2.5 in winter. The cities of Beijing, Zhengzhou, Tianjin, Tangshan, Xuzhou, Qingdao, Jinan, Jining, and Zibo had the highest emission intensities. Overall, emissions decreased from the city centers toward surrounding areas. The higher contributions of heavy-duty trucks meant that higher emissions appeared along highways in a vein-like distribution. These results provide a theoretical basis for the effective prevention and control of air pollution in the NCP.


Assuntos
Poluentes Atmosféricos , Poluição do Ar , Pequim , China , Cidades , Monitoramento Ambiental , Gasolina , Emissões de Veículos
11.
J Safety Res ; 73: 57-67, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-32563409

RESUMO

INTRODUCTION: Highway expansions and upgrades are often required to increase road network capacity. The widening of one side of a highway, referred to as 'one-side widening,' is sometimes implemented in these highway expansion projects. During one-side widening, to save costs, openings can be configured on existing medians (as opposed to removing the existing medians altogether). The median openings allow vehicles in the outer lanes to enter the inner lanes, but they also raise safety concerns and may require alternate open-median management strategies for traffic authorities. There is little existing research that has evaluated the safety effect of these open-median management strategies. METHOD: To bridge this gap, this study proposes a procedure that evaluates the safety of open-median management strategies for one-side widened highways. The proposed procedure was implemented through driving simulation experiments on a section of Binlai Freeway in Shandong, China. First, the minimum location requirements for median openings were determined by calculating the short length of the weaving segment. Then, simulation tests were carried out to observe driving performance and workload measures. RESULTS: The results indicate that the procedure successfully evaluates the safety effect of open-median management strategies for one-side widened freeways. It was also found that driving performance and workload are sensitive to the opening length and traffic flow. CONCLUSIONS: Therefore, median opening placement should be carefully selected in consideration of not only driving performance and workload but also traffic volume predictions. Practical Applications: The findings in this study can guide open-median management strategies for traffic safety one-side widened highways.


Assuntos
Acidentes de Trânsito/estatística & dados numéricos , Condução de Veículo/estatística & dados numéricos , Segurança/estatística & dados numéricos , China , Humanos
12.
J Basic Microbiol ; 59(9): 890-900, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31318074

RESUMO

Saccharopine dehydrogenase (EC 1.5.1.7) regulates the last step of fungal lysine biosynthesis. The gene (Fvsdh) encoding saccharopine dehydrogenase was identified and cloned from the whole genome of Flammulina velutipes. The genomic DNA of Fvsdh is 1257 bp, comprising three introns and four exons. The full-length complementary DNA of Fvsdh comprises 1107 bp with a deduced amino acid sequence of 368 residues. A 1,000-bp promoter sequence containing the TATA box, CAAT box, and several putative cis-acting elements was also identified. The results of tissue expression analysis showed that the expression level of the Fvsdh gene was higher in the pileus than in the stipe whether in the elongation or maturation stage. Further research showed that the lysine contents were 3.03 and 2.95 mg/g in maturation-pileus and elongation-pileus, respectively. In contrast, the lysine contents were 2.49 and 2.07 mg/g in elongation-stipe and maturation-stipe, respectively. To study the function of Fvsdh, we overexpressed Fvsdh in F. velutipes and found that Fvsdh gene expression was increased from 1.1- to 3-fold in randomly selected transgenic strains. The lysine contents were also increased from 1.12- to 1.3-fold in these five transformants, except for strain T3, in which the lysine contents were the same as the control. These results indicate that the expression of the Fvsdh gene can affect the lysine content of F. velutipes.


Assuntos
Flammulina/genética , Flammulina/metabolismo , Proteínas Fúngicas/genética , Lisina/biossíntese , Sacaropina Desidrogenases/genética , Sequência de Bases , Vias Biossintéticas/genética , Clonagem Molecular , Flammulina/classificação , Flammulina/crescimento & desenvolvimento , Proteínas Fúngicas/metabolismo , Expressão Gênica , Regulação Fúngica da Expressão Gênica , Filogenia , Regiões Promotoras Genéticas , Sacaropina Desidrogenases/metabolismo
13.
Zhongguo Gu Shang ; 31(7): 604-607, 2018 Jul 25.
Artigo em Chinês | MEDLINE | ID: mdl-30103581

RESUMO

OBJECTIVE: To investigate clinical effects of calcaneal fracture with closed reduction and minimally invasive plate fixation assisted with bidirectional distractor distraction. METHODS: From September 2015 to October 2016, 11 male patients(13 feet) with calcaneal fractures treated with bidirectional distractor distraction assisted with minimally invasive plate fixation were retrospectively studied. They were aged from 24 to 57 years old with an average of 36.4 years old;8 feet were type IIand 5 feet were type III according to Sanders classification. Postoperative incision, fracture healing, Böhler angle, Gissane angle were observed and Maryland scoring system was used to evaluate clinical effects. RESULTS: All fractures healed well without incision inflammation and incision disunion. All patients were followed up from 12 to 15 months with an average of 13.5 months. Böhler angle were improved from (9.6±7.3)° before operation to (20.2±4.6) ° at 1 year after operation, and had statistical meaning; Gissane angle increased from (92.7 ±8.5)° before operation to (121.7 ±7.6) ° at 1 year after operation. Maryland score at 1 year after operation was 88.79±8.25, and 11 feet got excellent results and 2 feet moderate. CONCLUSIONS: Bidirectional distractor distraction assisted with minimally invasive plate fixation could effectively fix calcaneal fractures, reduce postoperative complications, and get satisfied results of postoperative images and functional recovery. It is one of effective methods for treating Sanders II and III calcaneal fractures.


Assuntos
Calcâneo , Fraturas Ósseas , Adulto , Placas Ósseas , Fixação Interna de Fraturas , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Resultado do Tratamento , Adulto Jovem
14.
Ecotoxicol Environ Saf ; 135: 32-39, 2017 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-27677080

RESUMO

Limited information is available on the effects of gallium (Ga) and indium (In) on the growth of paddy rice. The Ga and In are emerging contaminants and widely used in high-tech industries nowadays. Understanding the toxicity and accumulation of Ga and In by rice plants is important for reducing the effect on rice production and exposure risk to human by rice consumption. Therefore, this study investigates the effect of Ga and In on the growth of rice seedlings and examines the accumulation and distribution of those elements in plant tissues. Hydroponic cultures were conducted in phytotron glasshouse with controlled temperature and relative humidity conditions, and the rice seedlings were treated with different levels of Ga and In in the nutrient solutions. The growth index and the concentrations of Ga and In in roots and shoots of rice seedlings were measured after harvesting. A significant increase in growth index with increasing Ga concentrations in culture solutions (<10mgGaL-1) was observed. In addition, the uptake of N, K, Mg, Ca, Mn by rice plants was also enhanced by Ga. However, the growth inhibition were observed while the In concentrations higher than 0.08mgL-1, and the nutrients accumulated in rice plants were also significant decreased after In treatments. Based on the dose-response curve, we observed that the EC10 (effective concentration resulting in 10% growth inhibition) value for In treatment was 0.17mgL-1. The results of plant analysis indicated that the roots were the dominant sink of Ga and In in rice seedlings, and it was also found that the capability of translocation of Ga from roots to shoots were higher than In. In addition, it was also found that the PT10 (threshold concentration of phytotoxicity resulting in 10% growth retardation) values based on shoot height and total biomass for In were 15.4 and 10.6µgplant-1, respectively. The beneficial effects on the plant growth of rice seedlings were found by the addition of Ga in culture solutions. In contrast, the In treatments led to growth inhibition of rice seedlings. There were differences in the phytotoxicity, uptake, and translocation of the two emerging contaminants in rice seedlings.


Assuntos
Gálio/análise , Hidroponia , Oryza/efeitos dos fármacos , Plântula/efeitos dos fármacos , Poluentes do Solo/análise , Biomassa , Gálio/toxicidade , Índio/análise , Índio/toxicidade , Oryza/química , Oryza/crescimento & desenvolvimento , Raízes de Plantas/química , Raízes de Plantas/efeitos dos fármacos , Raízes de Plantas/crescimento & desenvolvimento , Plântula/química , Plântula/crescimento & desenvolvimento , Poluentes do Solo/toxicidade
15.
Clin Lab ; 61(10): 1501-8, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26642712

RESUMO

BACKGROUND: Mounting evidence indicates that nuclear targeting by growth factors plays an indispensable role on their biological activities. Midkine (MK) is a multifunctional growth factor and has been discovered to play important roles in carcinogenesis. MK has been reported to localize to the nucleus and nucleolus of HepG2 cells and is involved in cell proliferation and apoptosis. METHODS: The interaction was reconfirmed by in vitro pull down and in vivo coimmunoprecipitation (Co-IP), also by the colocalization in the HepG2 cells. The proliferation and migration was determined by MTT and trans-well assay. RESULTS: PLSCR1 was identified as a novel MK-interacting protein. Notably, PLSCR1 interacted with MK in the cell nucleus and regulated hepatic carcinoma cell proliferation and migration. CONCLUSIONS: This study suggests that PLSCR1 positively regulates hepatic carcinoma cell proliferation and migration through interacting with MK, thus deepening our understanding on the regulation of midkine during hepatic carcinoma growth and metastasis.


Assuntos
Regulação Neoplásica da Expressão Gênica , Neoplasias Hepáticas/metabolismo , Fatores de Crescimento Neural/metabolismo , Proteínas de Transferência de Fosfolipídeos/metabolismo , Movimento Celular , Nucléolo Celular/metabolismo , Núcleo Celular/metabolismo , Proliferação de Células , Perfilação da Expressão Gênica , Células Hep G2 , Humanos , Neoplasias Hepáticas/genética , Microscopia de Fluorescência , Midkina , Metástase Neoplásica , Sais de Tetrazólio/química , Tiazóis/química , Técnicas do Sistema de Duplo-Híbrido , Cicatrização
16.
J Hazard Mater ; 286: 179-86, 2015 Apr 09.
Artigo em Inglês | MEDLINE | ID: mdl-25577320

RESUMO

Rice consumption is a major route of As exposure to human for the population of worldwide. This study investigates the effect of phytotoxicity and rice genotypes on the content and speciation of As in rice grains grown in different levels of As-elevated paddy soils from Taiwan. Three levels of As-elevated soils and six rice genotypes commonly planted in Taiwan were used for this study. The results indicate that As contents in grains of rice is not proportional to soil As concentrations and they were equal or higher in indica genotypes than japonica genotypes used in this study. It was also found that the As phytotoxicity not only reducing the grain yields but also the As concentrations in grain of rice. The predominant As species found in rice grains were dimethylarsinic acid (DMA) and arsenite. The concentrations of DMA increased with total As concentrations, wherggeas the arsenite remained in a narrow range from 0.1 to 0.3 mg kg(-1). Because of the lower toxicity of DMA than inorganic As species, the health risks may not be increased through consumption of rice even when total As content in the grains is increased.


Assuntos
Arsenitos/análise , Ácido Cacodílico/análise , Oryza/química , Poluentes do Solo/análise , Agricultura , Arsenitos/metabolismo , Arsenitos/toxicidade , Biomassa , Ácido Cacodílico/metabolismo , Ácido Cacodílico/toxicidade , Contaminação de Alimentos , Genótipo , Oryza/efeitos dos fármacos , Oryza/genética , Oryza/crescimento & desenvolvimento , Poluentes do Solo/metabolismo , Poluentes do Solo/toxicidade , Taiwan
17.
Artigo em Chinês | MEDLINE | ID: mdl-24044223

RESUMO

OBJECTIVE: Of this study was to prepare high sensitivity and high specificity of highly pathogenic H5N1 subtype avian influenza virus NS1 protein antibody and a preliminary assessment of its potency. METHODS: Construct pET-28a (+) recombinant vector containing the H5N1 subtype of avian influenza virus NS1 sequences of E. coli BL21 (DE3), induced expression of NS1 protein, NS1 recombinant protein was obtained by Ni-NTA column purified by affinity chromatography, and SDS-PAGE and Western Blot analysis. Purified protein antigen to immunize New Zealand white rabbits, obtained rabbit anti-NS1 serum, affinity-purified polyclonal antibodies. Using ELISA and Western Blot analysis of purified antibody titer and specificity. RESULTS: NS1 fusion protein was highly expressed in a purity of greater than 90%, with the fusion protein was used to immunize New Zealand white rabbits anti-NS1 polyclonal antibody titer of 1:80 000, and specific recognition of the H5N1 subtype of avian influenza virus NS1 protein. CONCLUSIONS: NS1 polyclonal antibodies to NS1 recombinant protein purified antigen, with better potency and specificity, and to prepare the conditions for the development of the H5N1 subtype of avian influenza virus detection kit.


Assuntos
Anticorpos Antivirais/biossíntese , Virus da Influenza A Subtipo H5N1/imunologia , Proteínas Recombinantes de Fusão/imunologia , Proteínas não Estruturais Virais/genética , Animais , Anticorpos Antivirais/imunologia , Escherichia coli/genética , Coelhos , Proteínas Recombinantes de Fusão/isolamento & purificação , Proteínas não Estruturais Virais/imunologia
18.
Artigo em Chinês | MEDLINE | ID: mdl-19927644

RESUMO

OBJECTIVE: To study possible impairment mechanisms of learning and memory abilities from unsaturated fatty acids in hippocampus of mice exposed to lead. METHODS: Forty-eight healthy mice were divided into 4 groups: low dose (0.625 g/L), middle dose (1.250 g/L) and high dose (2.500 g/L) of lead solution in diet and control group (distilled water). The mice in treatment groups were fed with lead solution every day while the mice in control group were fed with distilled water for 50 days. After learning and memory abilities were measured, the mice were killed and contents of oleic acid (C18:1), linoleic acid (C18:2), linolenic acid (C18:3), arachidonic acid (AA,C20:4), eicosapentaenoic acid (EPA,C20:5) and docosahexaenoic acid (DHA, C22:6 ) in hippocampus of mice were determined by high performance liquid chromatography (HPLC). RESULTS: (1) In the four training days, the mice treated with lead in the middle dose group and high dose group significantly increased the escape latencies compared with the mice treated with distilled water (P<0.05), and on the 4th day, the low dosage mice's escape latencies were delayed (P<0.05). The escape latencies of the 1st, 2nd, 3rd and 4th day had significantly positive linear relation with lead dose. Their relative coefficient in turn is r=0.973, 0.985, 0.929 and 0.936, indicating that lead harmed spatial memory of mice in Morris water maze (MWM). (2) The contents of C18:2 and AA were obviously enhanced in hippocampus of middle and high dosage (P<0.05); while there was evident decrease in the contents of C18:3, EPA and DHA (P<0.05); the content of C18:1 was decreased significantly in high dosage group (P<0.01). The mice's escape latencies had significantly negative linear relation with contents of C18:1, C18:3, EPA and DHA, while there was positive linear relation significantly with contents of C18:2 and AA. Their relative coefficient in turn was r=-0.901, -0.914, -0.893, -0.855, 0.936, 0.727. CONCLUSION: Lead interferes with the metabolism of hippocampus fatty acids and affects membrane function in hippocampus of mice, which might contribute to change of the synthesis, metabolism and release of central neurotransmitter and decrease of the learning and memory abilities.


Assuntos
Ácidos Graxos Insaturados/metabolismo , Hipocampo/metabolismo , Chumbo/toxicidade , Animais , Feminino , Hipocampo/efeitos dos fármacos , Masculino , Aprendizagem em Labirinto/efeitos dos fármacos , Camundongos , Camundongos Endogâmicos ICR
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...