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1.
J Biochem ; 167(1): 79-87, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-32193557

RESUMO

Disruption of circadian rhythms is associated with aberrant trophoblast migration and invasion in recurrent spontaneous abortion (RSA). This study aims to explore the functional role and the mechanisms of cryptochrome 2 (CRY2), a fundamental component of the circadian clock, in regulating trophoblast migration and invasion. Human extravillous trophoblast cell line HTR-8/SVneo was used as a cell model. Cell migration and invasion were examined using wound healing assay and Transwell assay, respectively. The mRNA and protein levels were determined using quantitative real-time polymerase chain reaction and western blot, respectively. Luciferase reporter assay and chromatin immunoprecipitation assay were performed to explore the interaction between c-Myc to the brain and muscle ARNT-like protein 1 (BMAL1) promoter. CRY2 was highly expressed in human villous specimens of RSA. Furthermore, CRY2 overexpression impaired migration and invasion in HTR-8/SVneo cells, whereas CRY2 knockdown yielded the opposite results. Mechanistically, c-Myc bound to the BMAL1 promoter and induced BMAL1 transcription, both of which further activated matrix metalloproteinase 2/9 (MMP2/9) and facilitated migration and invasion in HTR-8/SVneo cells. CRY2 inhibited c-Myc-BMAL1 pathway and impaired migration and invasion of HTR-8/SVneo cells. Collectively, these findings demonstrate that CRY2 suppresses trophoblast migration and invasion via inhibiting c-Myc-BMAL1-MMP2/9 pathway.

2.
Vet Microbiol ; 242: 108606, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32122609

RESUMO

Senecavirus A (SVA), previously called Seneca Valley virus, can cause vesicular lesions in sows and a sharp decline in neonatal piglet production. In this study, a SVA strain was isolated from a pig herd in Shandong Province in China and identified as SVV-CH-SD. The full genome was 7286 nucleotides (nt) in length and contained a single open reading frame (ORF) of 6546 nt, encoding a 2182 amino acid (aa). A phylogenetic analysis showed that the isolate shares highest sequence homology (98.52 %) with SVA strain USA-GBI26-2015. A genetic comparison of virulent and weakly virulent SVA strains showed that some amino acid residues may be associated with virulence. Animal challenge experiments showed that 90-100-day-old pigs inoculated with SVV-CH-SD intraorally and intranasally, intranasally, or intramuscularly developed low fever, blisters, and lameness. They had similar levels of neutralizing antibodies against SVA and viral loads in the serum and organs at 28 days post-CHallenge. However, 30-35- and 55-65-day-old pigs challenged with SVV-CH-SD showed no clinical signs, although anti-SVA neutralizing antibodies were detected. Our findings provide useful data for studying the pathogenesis and transmission of SVA in pigs.

3.
J Appl Clin Med Phys ; 2020 Mar 14.
Artigo em Inglês | MEDLINE | ID: mdl-32170991

RESUMO

PURPOSE: To investigate the set-up error and consequent dosimetric change in HexaPOD evo RT 6D couch under image-guided intensity-modulated radiotherapy (IG-IMRT) for primary malignant tumor of the cervical spine. METHODS: Ten cases with primary malignant tumor of the cervical spine were treated with intensity-modulated radiotherapy (IMRT) in our hospital from August 2013 to November 2014. The X-ray volumetric images (XVI) were scanned and obtained by cone-beam CT (CBCT). The six directions (6D) of set-up errors of translation and rotation were obtained by planned CT image registration. HexaPOD evo RT 6D couch made online correction of the set-up error, and then the CBCT was conducted to obtain the residual error. RESULTS: We performed set-up error and dosimetric analysis. First, for the set-up error analysis, the average error in three translation directions of 6D set-up error of the primary tumor of the cervical spine was <2 mm, whereas the single maximum error (absolute value) is 7.0 mm. Among average errors of rotation direction, Rotation X (RX) direction 0.67° ± 0.04°, Rotation Y (RY) direction 1.06° ± 0.06°, Rotation Z (RZ) direction 0.78° ± 0.05°; and the single maximum error in three rotation directions were 2.8°, 3.8°, and 2.9°, respectively. On three directions (X, Y, Z axis), the extended distance from clinical target volume (CTV) to planning target volume (PTV) was 3.45, 3.17, and 3.90 mm by calculating, respectively. Then, for the dosimetric analysis, the parameters, including plan sum PTV D98 and D95, planning gross tumor volume D98 and D95, V100% of the plan sum were significantly lower than the treatment plan. Moreover, Dmax of the spinal cord was significantly higher than the treatment plan. CONCLUSION: 6D set-up error correction system should be used for accurate position calibration of precise radiotherapy for patients with malignant tumor of the cervical spine.

4.
J Environ Manage ; 264: 110452, 2020 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-32217327

RESUMO

The power industry of China plays an important role in reducing the carbon dioxide (CO2) and air pollutant emissions. This paper focuses on synergistic CO2 and air pollution emission reduction by implementing technical and structural measures, and explores the processes aimed at achieving the co-benefits of carbon reduction and air pollution control effectively in the power industry at a local level. The results of the analysis show that the structural measures achieve overall co-benefits, and the technical measures have a good effect in reducing the air pollution, but simultaneously increase CO2 emissions because of the additional electricity used for running air pollution control devices (APCDs). Based on the analysis of the power generation incremental cost and pollutant emission reduction cost, cost-effective approaches to attain different emission reduction targets are proposed to the power industry in four case regions. These approaches can provide policy makers and stakeholders useful and relevant policy and operation recommendations to reduce CO2 and air pollution emissions cost-effectively and promote low carbon sustainability in the power industry of China.

5.
Virus Res ; 281: 197912, 2020 Mar 03.
Artigo em Inglês | MEDLINE | ID: mdl-32142743

RESUMO

The nucleocapsid (N) protein of porcine epidemic diarrhea virus (PEDV), the most important pathogen causing severe diarrhea in piglets, is a highly conserved structural protein. In this study, 5 monoclonal antibodies (McAbs) against the PEDV N-protein were prepared and identified. Three new epitopes, 56QIRWRMRRGERI67, 318GYAQIASLAPNVAALLFGGNVA VRE342 and 398HEEAIYDDV406, were firstly identified in the viral N-protein, by using McAbs 3F10, 6A11, and 1C9. The epitope 398HEEAIYDDV406 was deleted in SH strain (isolated by our lab) and different between CV777 and YZ strain (isolated by our lab). To study the characters of this epitope, four peptides were synthesized according to the sequence of SH and CV777 and used in the study. The result showed that the 398th amino acid maybe an important amino acid of the epitope. Biological information analysis showed that the three B cell linear epitopes are highly conserved among different PEDV isolates. In addition, McAb 1C9, which attached to the epitope 398HEEAIYDDV406, showed variant reactivity with PEDV CV777, SH, YZ and MS strains. McAb 1C9 reacted with PEDV strains CV777 and YZ, but not with SH which had a deletion from 399 to 410 amino acids in N-protein (No. MK841494). Among the three McAbs, 6A11, 3F10 and 1C9, only 6A11 reacted with porcine transmissible gastroenteritis virus (TGEV) in immunofluorescence assay, therefore the other two could be used to distinguish TGEV and PEDV. These mAbs and their defined epitopes may provide useful tool for the study of the PEDV N-protein structure and function, and facilitate the development of diagnostic methods for PEDV.

6.
Chem Res Toxicol ; 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-32200635

RESUMO

Halogenated amino acids and peptides are an emerging class of disinfection byproducts (DBPs), having been detected in drinking water and in washed food products. However, the toxicological significance of these emerging DBPs remains unclear. In this study, the cytotoxicity of eight halogenated tyrosyl compounds was investigated in Chinese hamster ovary (CHO) cells using real-time cell analysis (RTCA). Dihalogenated tyrosyl compounds are more cytotoxic than their monohalogenated analogues. The cytotoxicity of the dihalogenated compounds is associated with their ability to induce intracellular reactive oxygen species (ROS), suggesting that oxidative stress is an important toxicity pathway of these compounds. Pearson correlation analysis of the cytotoxicity (IC50 values) of these compounds with eight physicochemical parameters showed strong associations with their lipophilicity (logP) and reactivity (polarizability, ELUMO). Finally, cytotoxicity testing of the concentrated extracts of a chloraminated mixture of eight dipeptides with bromide or iodide showed the cytotoxicity of these mixtures in the order: iodinated peptides > brominated peptides ≥ chlorinated peptides. These results demonstrate that halogenated peptide DBPs are toxicologically relevant, and further research is needed to understand the implications of long-term exposure for human health.

7.
Exp Cell Res ; 389(2): 111897, 2020 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-32035951

RESUMO

Mucins are major macromolecular components of lung mucus that are mainly responsible for the viscoelastic property of mucus. MUC5AC is a major mucin glycoprotein that is hypersecreted in asthmatic individuals. Vascular endothelial growth factor (VEGF) has been implicated in inflammatory and airway blood vessel remodeling in asthmatics. Our previous studies indicate that VEGF upregulates MUC5AC expression by interacting with VEGF receptor 2 (VEGFR2). It has been shown that dexamethasone (Dex) downregulates MUC5AC expression; however, the underlying mechanisms have not been completely elucidated. Therefore, we sought to investigate the effect of Dex on MUC5AC expression induced by VEGF and study the underlying mechanisms. We tested the effects of Dex on VEGFR2 and RhoA activation, caveolin-1 expression, and the association of caveolin-1 and VEGFR2 in primary bronchial epithelial cells. Dex downregulated MUC5AC mRNA and protein levels in a dose- and time-dependent manner, and suppressed the activation of VEGFR2 and RhoA induced by VEGF. Additionally, Dex upregulated caveolin-1 protein levels in a dose- and time-dependent manner. Furthermore, phospho-VEGFR2 expression was decreased through overexpression of caveolin-1 and increased after caveolin-1 knockdown. Dex treatment attenuated the VEGF-decreased association of caveolin-1 and VEGFR2. Collectively, our findings suggest that Dex downregulates VEGF-induced MUC5AC expression by inactivating VEGFR2 and RhoA. Furthermore, decreased MUC5AC expression by Dex was related to the increased association of caveolin-1 with VEGFR2. Further studies characterizing these mechanisms are required to facilitate the development of improved treatment strategies for asthma.

8.
Exp Dermatol ; 2020 Feb 09.
Artigo em Inglês | MEDLINE | ID: mdl-32037580

RESUMO

Hair follicle central isthmus is surrounded by dense nerve endings and terminal Schwann cells (TSCs), forming a specialized sensory structure called lanceolate complexes. Extracellular matrix protein EGFL6 expressed from epidermis has been found closely associated with lanceolate complexes and important for proper alignment of nerve fibres and TSCs processes, and for proper response to light touch. However, how EGFL6 itself is specifically induced/deposited/maintained at the central isthmus remains to be elucidated. Previous reports and our results showed that nerve endings and TSCs docking at the central isthmus during hair follicle development occur before the specific depositing of EGFL6 protein. Furthermore, we found nude mice rarely maintain the lanceolate complex, and EGFL6 is lost in their aberrant hair follicle. Instead, reconstituted hair follicle in nude mice by stem cells chamber grafting assay expresses EGFL6 at the central isthmus area after hair follicle innervation. At last, long-term but not short-term cutaneous denervation leads to degeneration of TSCs and loss of EGFL6 expression. Together, our results demonstrate that EGFL6 expression in the central isthmus is dependent on the presence of TSCs, proposing that the interplay of epidermis and neuronal components is important for maintaining functional structure of lanceolate complexes.

9.
J Electrocardiol ; 59: 112-115, 2020 Feb 06.
Artigo em Inglês | MEDLINE | ID: mdl-32062379

RESUMO

Pheochromocytoma is a neuroendocrine tumor of the adrenal gland that secretes excess amount of catecholamine, which in turn leads to extremely varied clinical manifestations, such as hypertension, headache, sweating and palpitations. This eventually leads to delay or mistakes in diagnosing the disease. This could be fatal due to misdiagnosis as it receives a totally different treatment. We herein reported a case of pheochromocytoma with recurrent ventricular tachycardia. A literature review was conducted to investigate the clinical features of these patients.

10.
Fitoterapia ; 142: 104499, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32058049

RESUMO

3-O-[(E)-4-(4-cyanophenyl)-2-oxobut-3-en-1-yl] kaempferol is a novel lead compound to discover anti-diabetic and anti-obesity drugs. The present study reported the scaffold hopping of the lead compound to obtain a new isoxazole derivative, C45, which has improved glucose consumption at the nanomolar level (EC50 = 0.8 nM) in insulin resistant (IR) HepG2 cells. Western blotting showed that C45 markedly enhanced the phosphorylation of AMPK (AMP-activated protein kinase) and reduced the levels of the gluconeogenesis key enzymes PEPCK (phosphoenolpyruvate carboxykinase) and G6Pase (glucose 6-phosphatase) in HepG2 cells. The potential molecular mechanism of C45 may be activation of the AMPK/PEPCK/G6Pase pathways. We concluded that C45 might be a valuable candidate to discover anti-diabetic drugs.

11.
Sensors (Basel) ; 20(3)2020 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-32012874

RESUMO

Bin-picking of small parcels and other textureless planar-faced objects is a common task at warehouses. A general color image-based vision-guided robot picking system requires feature extraction and goal image preparation of various objects. However, feature extraction for goal image matching is difficult for textureless objects. Further, prior preparation of huge numbers of goal images is impractical at a warehouse. In this paper, we propose a novel depth image-based vision-guided robot bin-picking system for textureless planar-faced objects. Our method uses a deep convolutional neural network (DCNN) model that is trained on 15,000 annotated depth images synthetically generated in a physics simulator to directly predict grasp points without object segmentation. Unlike previous studies that predicted grasp points for a robot suction hand with only one vacuum cup, our DCNN also predicts optimal grasp patterns for a hand with two vacuum cups (left cup on, right cup on, or both cups on). Further, we propose a surface feature descriptor to extract surface features (center position and normal) and refine the predicted grasp point position, removing the need for texture features for vision-guided robot control and sim-to-real modification for DCNN model training. Experimental results demonstrate the efficiency of our system, namely that a robot with 7 degrees of freedom can pick randomly posed textureless boxes in a cluttered environment with a 97.5% success rate at speeds exceeding 1000 pieces per hour.

12.
Sci Transl Med ; 12(530)2020 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-32051229

RESUMO

Sudden death could be the first symptom of patients with arrhythmogenic cardiomyopathy (AC), a disease for which clinical indicators predicting adverse progression remain lacking. Recent findings suggest that metabolic dysregulation is present in AC. We performed this study to identify metabolic indicators that predicted major adverse cardiac events (MACEs) in patients with AC and their relatives. Comparing explanted hearts from patients with AC and healthy donors, we identified deregulated metabolic pathways using quantitative proteomics. Right ventricles (RVs) from patients with AC displayed elevated ketone metabolic enzymes, OXCT1 and HMGCS2, suggesting higher ketone metabolism in AC RVs. Analysis of matched coronary artery and sinus plasma suggested potential ketone body synthesis at early-stage AC, which was validated using patient-derived induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) in vitro. Targeted metabolomics analysis in RVs from end-stage AC revealed a "burned-out" state, with predominant medium-chain fatty acid rather than ketone body utilization. In an independent validation cohort, 65 probands with mostly non-heart failure manifestations of AC had higher plasma ß-hydroxybutyrate (ß-OHB) than 62 healthy volunteers (P < 0.001). Probands with AC with MACE had higher ß-OHB than those without MACE (P < 0.001). Among 94 relatives of probands, higher plasma ß-OHB distinguished 25 relatives having suspected AC from nonaffected relatives. This study demonstrates that elevated plasma ß-OHB predicts MACE in probands and disease progression in patients with AC and their clinically asymptomatic relatives.

13.
J Gene Med ; : e3169, 2020 Feb 06.
Artigo em Inglês | MEDLINE | ID: mdl-32028542

RESUMO

BACKGROUND: microRNA (miR)-mediated post-transcriptional repression has been reported in the process of chondrocyte dysfunction. The present study aimed to investigate the molecular mechanisms underlying in oleanolic acid (OLA)-prevented interleukin (IL)-1ß-induced chondrocyte dysfunction via the miR-148-3p/fibroblast growth factor-2 (FGF-2) signaling pathway. METHODS: Candidate miRs were filtrated using miR microarray assays in chondrocytes with or without IL-1ß stimulation. Gene expression of candidate miRs and protein expression of FGF2 were analyzed using a quantitative reverse transcriptase-polymerase chain reaction and western blotting, respectively. Cell growth was evaluated using cell counting kit-8 assays. Cell apoptosis was detected using Annexin V-fluorescein isothiocyanate double staining. RESULTS: Treatment with OLA counteracted IL-1ß-evoked chondrocyte growth inhibition, apoptosis, caspase3 production, and release of malondialdehyde (MDA) and 8-hydroxy-2'-deoxyguanosine. Additionally, FGF2 protein expression levels elevated by IL-1ß were down-regulated by OLA and transfection with miR-148-3p mimics. IL-1ß-induced down-regulation of miR-148-3p in chondrocytes was evaluated by OLA administration. Bioinformatics algorithms and experimental measurements indicated that FGF2 might be a direct target of miR-148-3p. miR-148-3p mimics exhibited equal authenticity of OLA to protect against IL-1ß-induced chondrocyte dysfunction. CONCLUSIONS: Our present findings highlight a protective effect of OLA on IL-1ß-induced chondrocyte dysfunction, and a novel signal cascade comprising the miR-148-3p/FGF2 signaling pathway might be a potential therapeutic target of OLA with respect to preventing the progression of osteoarthritis.

14.
J Affect Disord ; 264: 187-192, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-32056749

RESUMO

BACKGROUND: Poor mental health status among both pregnant and postpartum women is commonly reported worldwide. The associations between mental health outcomes and giving birth to the second child since the implementation of China's universal two-child policy have not been identified. METHODS: A large-scale based mental health survey was conducted between March 2017 and December 2018 in Suzhou, China. The survey evaluated the symptoms of anxiety, hypomania, depression and poor sleep quality among both pregnant and postpartum women. RESULTS: A total of 3113 questionnaires were collected, the prevalence of anxiety, hypomanic and depressive symptoms and poor sleep quality in our sample were 3.2% (95%CI: 2.6%-3.9%), 51.7% (95%CI: 49.9%-53.4%), 12.4% (95%CI: 11.3%-13.6%) and 37.8% (95%CI: 36.0%-39.5%), respectively. Logistic regression showed that giving birth to the second child was positively associated with women's age, and was negatively correlated with higher educational level and living in rented housing. Women with the second pregnancy or child were positively associated with anxiety symptoms in the whole sample (OR = 1.75, 95%CI: 1.11-2.75) and among prenatal women (OR = 2.11, 95%CI: 1.16-3.83), while it was inversely correlated with depressive symptoms among postpartum women (OR = 0.63, 95%CI: 0.41-0.99). CONCLUSIONS: Women giving birth a second time were more prone to have anxiety symptoms among the prenatal women and the whole sample, and less likely to have depressive symptoms among the postpartum women. Efficacious measures and interventions are essential to improve maternal mental health.

15.
Aging (Albany NY) ; 12(1): 996-1010, 2020 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-31913855

RESUMO

Cardiac fibrosis (CF) is regulated by multiple factors, including transforming growth factor ß1 (TGFß1) and non-coding RNAs. Thrombospondin 1 (TSP1) is a physiologic regulator of TGFß activation. Here, we performed microarray analyses on mRNAs and lncRNAs differentially-expressed in the CF and normal rat hearts. KEGG signaling annotation and GO enrichment analyses were performed to validate the roles of extracellular matrix (ECM) and TSP1-enhanced TGFß activation in CF. The co-expression network between differentially-expressed lncRNAs and ECM-related factors was constructed to identify candidate lncRNAs and miRNAs. We found that lncRNA Homo sapiens ring finger protein 7 (lnc RNF7) was significantly correlated with TSP1 and ECM. Lnc RNF7 silence could attenuate isoproterenol (ISP)-induced CF in rat heart in vivo and in rat cardiac fibroblasts in vitro. Moreover, angiotensin II (Ang II) -induced CF in rat cardiac fibroblasts could also be attenuated by Lnc RNF7 silence. Furthermore, miR-543 could simultaneously target lnc RNF7 and 3' UTR of TSP1. Lnc RNF7 silence suppressed, while miR-543 inhibition promoted TSP1 protein and TGFß activation, as well as ECM markers expression. The effects of lnc RNF7 silence was significantly reversed by miR-543 inhibition. In conclusion, CF progression might be regulated by lnc RNF7/miR-543 axis via TSP1-mediated TGFß activation.

16.
Vet Microbiol ; 240: 108511, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31902508

RESUMO

Since late 2010, highly virulent PEDV G2-genotype strains have emerged globally extracting heavy losses on the pork industries of numerous countries. We investigated the characteristics of a field strain of PEDV (PEDV strain SH) isolated from a piglet with severe diarrhea on a farm in Shanghai China. Whole genome sequencing and analysis revealed that the SH strain belonged to subtype G2b and has a unique 12-aa deletion (aa 399-410) including the antigenic epitope NEP-1C9 (aa 398-406) of the N protein. PEDV SH strain is highly pathogenic to challenged newborn piglets, resulting in 100 % morbidity and mortality. Pathological examination revealed significant villus atrophy in the jejuna of infected piglets. Mice inoculated with inactivated PEDV SH produced antibodies against the N protein, but no antibodies against the deletions. These results illustrated that deletion of the NEP-1C9 epitope had no effect on the immunogenicity or pathogenicity of PEDV, providing evidence of the necessity to monitor the genetic diversity of the virus. Our study also contributes to development of candidate for vaccines and diagnostics that could differentiate pigs seropositive due to vaccination by conventional strains from wild virus infection.

17.
Chin Med J (Engl) ; 133(4): 424-434, 2020 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-31977553

RESUMO

BACKGROUND: Traditional tissue engineering methods to fabricate urinary tract patch have some drawbacks such as compromised cell viability and uneven cell distribution within scaffold. In this study, we combined three-dimensional (3D) bioprinting and tissue engineering method to form a tissue-engineered urinary tract patch, which could be employed for the application on Beagles urinary tract defect mode to verify its effectiveness on urinary tract reconstruction. METHODS: Human adipose-derived stem cells (hADSCs) were dropped into smooth muscle differentiation medium to generate induced microtissues (ID-MTs), flow cytometry was utilized to detect the positive percentage for CD44, CD105, CD45, and CD34 of hADSCs. Expression of vascular endothelial growth factor A (VEGFA) and tumor necrosis factor-stimulated gene-6 (TSG-6) in hADSCs and MTs were identified by Western blotting. Then the ID-MTs were employed for 3D bioprinting. The bioprinted structure was encapsulated by transplantation into the subcutaneous tissue of nude mice for 1 week. After retrieval of the encapsulated structure, hematoxylin and eosin and Masson's trichrome staining were performed to demonstrate the morphology and reveal collagen and smooth muscle fibers, integral optical density (IOD) and area of interest were calculated for further semi-quantitative analysis. Immunofluorescent double staining of CD31 and α-smooth muscle actin (α-SMA) were used to reveal vascularization of the encapsulated structure. Immunohistochemistry was performed to evaluate the expression of interleukin-2 (IL-2), α-SMA, and smoothelin of the MTs in the implanted structure. Afterward, the encapsulated structure was seeded with human urothelial cells. Immunofluorescent staining of cytokeratins AE1/AE3 was applied to inspect the morphology of seeded encapsulated structure. RESULTS: The semi-quantitative assay showed that the relative protein expression of VEGFA was 0.355 ±â€Š0.038 in the hADSCs vs. 0.649 ±â€Š0.150 in the MTs (t = 3.291, P = 0.030), while TSG-6 expression was 0.492 ±â€Š0.092 in the hADSCs vs. 1.256 ±â€Š0.401 in the MTs (t = 3.216, P = 0.032). The semi-quantitative analysis showed that the mean IOD of IL-2 in the MT group was 7.67 ±â€Š1.26, while 12.6 ±â€Š4.79 in the hADSCs group, but semi-quantitative analysis showed that there was no statistical significance in the difference between the two groups (t = 1.724, P = 0.16). The semi-quantitative analysis showed that IOD was 71.7 ±â€Š14.2 in non-induced MTs (NI-MTs) vs. 35.7 ±â€Š11.4 in ID-MTs for collagen fibers (t = 3.428, P = 0.027) and 12.8 ±â€Š1.9 in NI-MTs vs. 30.6 ±â€Š8.9 in ID-MTs for smooth muscle fibers (t = 3.369, P = 0.028); furthermore, the mean IOD was 0.0613 ±â€Š0.0172 in ID-MTs vs. 0.0017 ±â€Š0.0009 in NI-MTs for α-SMA (t = 5.994, P = 0.027), while 0.0355 ±â€Š0.0128 in ID-MTs vs. 0.0035 ±â€Š0.0022 in NI-MTs for smoothelin (t = 4.268, P = 0.013), which indicate that 3D bioprinted structure containing ID-MTs could mimic the smooth muscle layer of native urinary tract. After encapsulation of the urinary tract patch for additional cell adhesion, urothelial cells were seeded onto the encapsulated structures, and a monolayer urothelial cell was observed. CONCLUSION: Through 3D bioprinting and tissue engineering methods, we provided a promising way to fabricate tissue-engineered urinary tract patch for further investigation.

18.
Technol Cancer Res Treat ; 19: 1533033819901115, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31964245

RESUMO

Lung cancer is the most common cancer type with increasingly high incidence. MicroRNAs provide the potential biomarkers for lung cancer treatment. Thus, we aimed to investigate the function of microRNA-425-5p in lung cancer development and the underlying mechanisms. MicroRNA-425-5p overexpression inhibited A549 lung cancer cell proliferation in vitro and in vivo. On the other hand, microRNA-425-5p inhibition increased A549 proliferation. Mechanistically, the underlying mechanism by which microRNA-425-5p inhibits lung cancer cell growth was mediated through its ability in targeting and downregulating the TFIIB-related factor 2. Our results for the first time identified microRNA-425-5p as a tumor suppressor in lung cancer. Thus, microRNA-425-5p may serve as a potential therapeutic target for lung cancer.

20.
IEEE Trans Cybern ; 2020 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-31976921

RESUMO

This article discusses the synchronization problem for a class of multiple delayed neural networks (MDNNs) with a directed switching topology by using an event-triggering \nobreak strategy. First, a new differential inequality with delay is shown, which is a generalization of Halanay-type inequalities. Then, the sufficient conditions of event-based synchronization (quasisynchronization) for MDNN with sequentially connected topology are obtained by using this inequality and the iterative method. Meantime, we prove that Zeno behavior can be avoided under the designed event-triggering rules. As an extension, MDNN with jointly connected topology is also discussed. Finally, a numerical example is listed to illustrate the results in theory analysis.

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