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1.
Biomarkers ; 25(2): 149-156, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31922440

RESUMO

Context: We previously reported a novel tumour associated antigen (TTA) with molecular weight around 48 kDa and identified the novel TTA as a fragment derived from human DNA-topoiomerase I (TOP1). We termed the novel TAA as TOPO48 and termed autoantibody against the TAA as anti-TOPO48 autoantibody.Objective: To explore the clinical significance of anti-TOPO48 autoantibody in patients with colorectal carcinoma (CRC).Materials and methods: Serum levels of the autoantibody in patients with CRC or benign tumours and healthy volunteers were measured with a specific ELISA.Results: CRC patients at early stage had higher frequency of positive levels of the autoantibody and CRC patients with positive autoantibody levels had higher overall survival rate than those with negative autoantibody levels.Conclusion: The autoantibody is a potential biomarker for early diagnosis and favourable prognosis of CRC.

2.
Cell Immunol ; 347: 104007, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31732123

RESUMO

Previously, we reported a novel tumor-associated antigen (TAA) derived from human DNA-topoiomerase I (TOP 1). In the present study, we demonstrated that the autoantibody against the TAA could be a potential biomarker in the early diagnosis and favorable prognosis of patients with breast cancer (BC). To understand the survival benefits in BC patients, we investigated whether the autoantibody could induce antibody-dependent cellular cytotoxicity activities (ADCC) against breast cancer cells in vitro. We found that the autoantibody exhibited significant ADCC activities that destroyed breast cancer MCF-7 and MDA-MB-231cells with peripheral blood mononuclear cells (PBMCs). The ADCC activities of the autoantibody were significantly correlated with the number of natural killer (NK) cells, NKT cells, and CD4+/CD8+ T cells. Accordingly, our findings showed that the autoantibody not only represented an early index of immune response to the TAA, but also was involved in host immune defense mechanisms that initiated the destruction of cancer cells.

3.
J Food Biochem ; 43(12): e13061, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31612542

RESUMO

The capsaicin in hot peppers is an important biological active substance that is widely used in food and medicine. In this work, six capsaicin derivatives such as N-(4-Hydroxy-3-acetophenone benzyl)acrylamide (A), 2-hydroxy-3-(octyloxy)phenyl-5-acrylamidemethylbenzene phenyl methanone (B), N-(2,5-dihydroxybenzene)acetamide (C), N-(5-acetamidemethyl benzene-2,4-dihydroxybenzene)acetamide (D), 4-acetamideme thylbenzene-2-benzylphenol (E), and N-(2-methyl-4-hydroxy-5-methylthiobenzene)acetamide (F) were synthesized via the Friedel-Crafts (F-C) alkylation reaction and were characterized using IR, 1 H NMR, and HRMS. The antioxidant activity of compounds was evaluated using the reducing power and DPPH radical (DPPH·) scavenging assays, and Vitamin C (Vc) was used as a control. The antibacterial activity was tested using minimum inhibition concentration (MIC) and antibacterial rate assays, and Escherichia coli and Staphylococcus aureus were used as the tested strain. The results showed that all six capsaicin derivatives had certain antioxidant and antibacterial activities, and the activities increased with increasing mass concentration. The best properties were obtained for compounds C and F; the antioxidant activity of compound C was similar to Vc and the MIC of compound F was 0.0313 mg/ml, its antibacterial rate was greater than 99% at 3 mg/ml. PRACTICAL APPLICATIONS: As a vegetable, peppers can be eaten fresh or processed to other forms such as pepper powder or pepper jam, and it is very popular because of its long history, unique flavor, and special functions. Our current study shows that capsaicin derivatives have good antioxidant and antibacterial activities, and therefore, the present study of capsaicin derivatives with good activity provides a good foundation for future applications in natural food additives and medicine.

5.
Arch Gynecol Obstet ; 299(1): 229-237, 2019 01.
Artigo em Inglês | MEDLINE | ID: mdl-30341503

RESUMO

PURPOSE: To examine the clinical significance of an autoantibody (AAb) against a novel tumor-associated antigen (TAA) derived from human DNA-topoisomerase I, termed as TOPO48 AAb, and peripheral blood survivin-expressing circulating cells (CCC) in patients with early stage endometrial cancer (EC). METHODS: Blood samples were collected from 80 patients with early stage EC and 80 age-matched healthy subjects. Plasma levels of the TOPO48 AAb were measured with a specific antibody capture enzyme-linked immunosorbent assay (ELISA) and blood survivin-expressing CCC assessed with a reverse transcription-polymerase chain reaction products based on a hybridization-enzyme-linked immunosorbent assay (RT-PCR-ELISA). Sixty patients were followed up for 36 months after the initial assay test. RESULTS: There were 75% and 60% samples with positive levels of the TOPO48 AAb and survivin-expressing CCC in the cancer patients, respectively. However, the cumulative positive rate of combination of the two markers was increased to 93.3% with 0.927 (95% CI 0.871-0.984) of area under the curve (AUC) in receiver operating characteristic (ROC) curve analysis. During the follow-up period, patients with positive TOPO48 AAb but negative surviving-expressing CCC had a higher survival rate and a longer survival time than those with negative AAb but positive CCC (P = 0.01). CONCLUSIONS: The combination of TOPO48 AAb and survivin-expressing CCC may be used as a novel recipe to improve the efficiency of early diagnosis and provide more accurate prognostic prediction in patients with early stage EC.


Assuntos
Autoanticorpos/sangue , DNA Topoisomerases Tipo I/sangue , Neoplasias do Endométrio/sangue , Células Neoplásicas Circulantes/metabolismo , Survivina/sangue , Adulto , Antígenos de Neoplasias , Neoplasias do Endométrio/mortalidade , Neoplasias do Endométrio/patologia , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Pessoa de Meia-Idade , Células Neoplásicas Circulantes/patologia , Prognóstico , Taxa de Sobrevida
6.
Zhongguo Zhong Yao Za Zhi ; 43(15): 3051-3057, 2018 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-30200698

RESUMO

Cerebral malaria (CM) is the leading cause of death in children under 5 years in Africa, severe neurological sequelae may occur in surviving children. Although artesunate has made breakthrough progress in the clinical treatment of CM, the clinical problems of high mortality and high morbidity have not yet been completely resolved. In this study, an experimental cerebral malaria (ECM) model was established by infecting C57BL/6 mice with Pb ANKA (Plasmodium berghei ANKA) to compare parasitemia level, survival rates, and rapid murine coma behavior scale scores, cerebral microvascular obstruction, haemozoin deposition in the liver, body temperature and weight to investigate the anti-cerebral malaria effect of the artesunate compound combination. The results showed that the artesunate compound combination could improve the survival rate of Pb ANKA-infected mice, reduce the level of parasitemia, effectively improve the symptoms of ECM neurological injury, reduce cerebrovascular obstruction and haemozoin deposition in the liver, and also significantly improve body temperature, weight and other basic indicators. The results showed that the artesunate compound combination improved the pathological changes and neurological damage caused by CM. It is expected to provide a theoretical basis for human cerebral malaria patients in clinical adjuvant therapy.


Assuntos
Antimaláricos/farmacologia , Artesunato/farmacologia , Malária Cerebral/tratamento farmacológico , Animais , Modelos Animais de Doenças , Camundongos , Camundongos Endogâmicos C57BL , Plasmodium berghei
7.
Zhongguo Zhong Yao Za Zhi ; 43(16): 3397-3403, 2018 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-30200747

RESUMO

Malaria is still the most severe strain of the human malaria parasites, and malaria disease is life-threatening which can result in severe anemia and cerebral malaria, especially in children in tropical Africa. Previous studies have shown that artemisinin and its derivatives could selectively kill erythrocytic stage of malaria and have a greater impact on the ring period. In recent years, there have been new findings of its mechanism continually. However, the concentration of artemisinin and its derivatives used in these studies can reach 50 to 80 times the half-inhibitory concentration in vitro. In this study, the international standard strain 3D7 of Plasmodium falciparum was used to culture in vitro. After half-inhibitory concentration of dihydroartemisinin was treated, the morphological changes of P. falciparum intraerythrocytic stage were observed, and then the 3D7 life cycle and effects of different developmental stages after dosing was explored. The 3D7 strain of P. falciparum was continuously synchronised more than 3 times. And dihydroartemisinin (DHA) at half maximal inhibitory concentration (10 nmol·L⁻¹) was administered for 6 hours after the last synchronization, and 3 life cycles were continuously observed (132 h). The results showed that compared with the parasites untreated by DHA, there was a noticeable delay in the life cycle of at least 36 h, indicating that the growth of 3D7 was significantly inhibited by DHA (P<0.001), and the rate of ring formation was significantly reduced (P<0.05). The trophozoites were abnormal in shape, such as shrink in size, and the number of merozoites in schizonts was significantly decreased (P<0.05). These results suggested that non-killing concentrations of DHA (meaning parasites can be inhibited but not killed) can significantly inhibit the growth of P. falciparum, which may not only affect the ring stage, but also have an impact on other stages of the P. falciparum.


Assuntos
Antimaláricos/farmacologia , Artemisininas/farmacologia , Plasmodium falciparum/efeitos dos fármacos , Humanos
9.
Cancer Lett ; 427: 74-84, 2018 07 28.
Artigo em Inglês | MEDLINE | ID: mdl-29702194

RESUMO

Increasing evidence has shown that microRNAs (miRNAs) play a significant functional role by directly regulating respective targets in cancer stem cell (CSC)-induced non-small cell lung cancer (NSCLC) progression and resistance to therapy. In this study, we found that hsa-miR-124a was downregulated during spheroid formation of the NSCLC cell lines SPC-A1 and NCI-H1650 and NSCLC tissues compared with normal lung cells and tissues. Patients with lower hsa-miR-124a expression had shorter overall survival (OS) and progression free survival (PFS). Moreover, ubiquitin-specific protease 14 (USP14) was confirmed to be a direct target of hsa-miR-124a. Furthermore, concomitant low hsa-miR-124a expression and high USP14 expression were correlated with a shorter median OS and PFS in NSCLC patients. Cellular functional analysis verified that the tumor suppressor hsa-miR-124a negatively regulated cell growth and self-renewal, and promoted apoptosis and gefitinib sensitivity of lung cancer stem cells by suppressing its target gene USP14. Our results provide the first evidence that USP14 is a direct target of hsa-miR-124a, and that hsa-miR-124a inhibits stemness and enhances the gefitinib sensitivity of NSCLC cells by targeting USP14. Thus, hsa-miR-124a and USP14 may be useful as tumor biomarkers for the diagnosis and treatment of NSCLC.


Assuntos
Carcinoma Pulmonar de Células não Pequenas/genética , Gefitinibe/farmacologia , Neoplasias Pulmonares/genética , MicroRNAs/genética , Células-Tronco Neoplásicas/metabolismo , Ubiquitina Tiolesterase/genética , Antineoplásicos/farmacologia , Apoptose/efeitos dos fármacos , Apoptose/genética , Carcinoma Pulmonar de Células não Pequenas/metabolismo , Carcinoma Pulmonar de Células não Pequenas/patologia , Linhagem Celular Tumoral , Proliferação de Células/genética , Sobrevivência Celular/efeitos dos fármacos , Sobrevivência Celular/genética , Regulação Neoplásica da Expressão Gênica , Genes Supressores de Tumor , Humanos , Estimativa de Kaplan-Meier , Neoplasias Pulmonares/metabolismo , Neoplasias Pulmonares/patologia , MicroRNAs/metabolismo , Células-Tronco Neoplásicas/efeitos dos fármacos , Esferoides Celulares/metabolismo , Ubiquitina Tiolesterase/metabolismo
10.
Cancer Lett ; 423: 86-94, 2018 06 01.
Artigo em Inglês | MEDLINE | ID: mdl-29548818

RESUMO

Metastatic invasion is the primary cause of treatment failure for GBM. EMT is one of the most important events in the invasion of GBM; therefore, understanding the molecular mechanisms of EMT is crucial for the treatment of GBM. In this study, high expression of DRR1 was identified to correlate with a shorter median overall and relapse-free survival. Loss-of-function assays using shDRR1 weakened the invasive potential of the GBM cell lines through regulation of EMT-markers. The expressions of p-AKT were significantly decreased after DRR-depletion in SHG44 and U373 cells. Moreover, the invasion was inhibited by the AKT inhibitor, MK-2206. The expression of Vimentin, N-cadherin, MMP-7, snail and slug was significantly inhibited by MK-2206, while the expression of E-cadherin was upregulated. Our results provide the first evidence that DRR1 is involved in GBM invasion and progression possibly through the induction of EMT activation by phosphorylation of AKT.


Assuntos
Neoplasias Encefálicas/metabolismo , Glioblastoma/metabolismo , Proteínas Nucleares/genética , Proteínas Nucleares/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Neoplasias Encefálicas/genética , Linhagem Celular Tumoral , Movimento Celular , Transição Epitelial-Mesenquimal , Feminino , Regulação Neoplásica da Expressão Gênica , Genes Supressores de Tumor , Glioblastoma/genética , Humanos , Masculino , Invasividade Neoplásica , Fosforilação , Prognóstico , Análise de Sobrevida , Regulação para Cima
11.
Zhongguo Zhong Yao Za Zhi ; 42(23): 4548-4555, 2017 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-29376251

RESUMO

Cerebral malaria (CM), a severe neurological syndrome caused by Plasmodium falciparum infection, is a serious life-threatening disease with a high mortality. Survivors' persistent brain injury is manifested as long-term neurocognitive disorders. The main neuropathological feature of CM is the sequestration of parasited red blood cells (pRBCs) in cerebral microvessels. Other neuropathological features of CM include petechial hemorrhage in the brain parenchyma, annular hemorrhage, extensive brain endothelial cell activation, and focal endothelial cell injury and necrosis. However, its pathogenesis is still not clear. Currently, some studies have suggested that the pathogenesis of cerebral malaria mainly include pRBC adhesion, inflammatory reaction cascade, vascular leakage damage and brain hypoxia. Studies have shown that the biomarkers currently used as diagnostic and prognostic markers for CM include C-X-C motif chemokine ligand 10 (CXCL10), CXC chemokine ligand 4 (CXCL4), angiopoietin (Ang). In this paper, we systematically summarize the basic and clinical research for cerebral malaria in recent years and the latest literatures for drug studies, and focused on the advance of studies on cerebral malaria and its immunologic mechanism in the recent three years in the aspects of cytokines, immune cells, regulatory factors and biomarkers, so as to provide references for relevant studies.


Assuntos
Encéfalo/patologia , Eritrócitos/parasitologia , Inflamação/parasitologia , Malária Cerebral/patologia , Biomarcadores/sangue , Quimiocinas/sangue , Humanos , Malária Cerebral/imunologia
12.
Yao Xue Xue Bao ; 51(8): 1263-70, 2016 08.
Artigo em Chinês | MEDLINE | ID: mdl-29898356

RESUMO

This study was designed to investigate the activity of Shenlian tablet in stabilization of the atherosclerosis (As) plaque in apoE(-/-) mice and explore the mechanisms. Rat peritoneal mast cells were randomly allocated and treated with Shenlian tablet (100, 50, 25, 12.5 mg·L(-1)) or cromoglicate sodium (200 µg·L(-1)) for 2 h before exposure to substance P. Histamine, tryptase, IL-1ß and NF-κB were measured in the cell culture supernatant by ELISA assay. The plaque formation was induced by common carotid artery cannula method combined with high-fat diet in apoE(-/-) mice, and the plaque instability was induced by substance P through local mast cell degranulation. Mice were divided into eight groups that included the model 1 (M1, sham-operated group), M2 (carotid artery cannula combined with high-fat diet), M3 (M2 combined with substance P 0.5 µg/mouse, Shenlian extract (95, 190 and 380 mg·kg(-1)·d(-1)), atorvastatin (2.6 mg·kg-1·d(-1)) and normal control group. Total cholesterol (TC), high-density lipoprotein (HDL-C), high-sensitivity C-reactive protein (hs CRP), matrix metalloproteinases 9 (MMP-9) and histamine were measured by ELISA. Thickness, plaque area, mast cell degranulation were observed by hematoxylin and eosin staining, toluidine blue staining. CD117 antigen expression were observed by confocal microscopy. Intracellular phosphorylation was detected using the Bio-Plex 6-plex phosphoprotein assay kit. The results show that the mast cell membrane was stabilized by Shenlian tablet. Histamine, tryptase, interleukin l-ß and NF-κB exhibited a significantly reduction in the Shenlian tablet-treated group (P < 0.05 or P < 0.01). Substance P significantly enhanced activation and degranulation of adventitial mast cells. In addition, it increased adventitia inflammatory cells infiltration and promoted intraplaque hemorrhages in apoE(-/-) mice model group. The proliferation, degranulation and inflammation of mast cell were significantly inhibited by Shenlian tablet. On the other hand, the same treatment decreased hs-CRP, MMP-9 and histamine in serum. IκB, p38 MAPK phosphorylation, intraplaque hemorrhage and collagen degradation were reduced in the presence of Shenlian tablet, which increased the stability of the As plaque. The results show that the vulnerable plaque model induced by mast cell activation in adventitia was established. Shenlian tablet exhibited a protective effect in this model. Shenlian tablet may increase the plaque stability via inhibition of mast cell-mediated inflammatory response.


Assuntos
Degranulação Celular , Medicamentos de Ervas Chinesas/farmacologia , Mastócitos/efeitos dos fármacos , Placa Aterosclerótica/tratamento farmacológico , Túnica Adventícia/citologia , Animais , Atorvastatina/farmacologia , Proteína C-Reativa/metabolismo , Dieta Hiperlipídica , Modelos Animais de Doenças , Histamina/metabolismo , Interleucina-1beta/metabolismo , Lipoproteínas HDL/metabolismo , Mastócitos/fisiologia , Metaloproteinase 9 da Matriz/metabolismo , Camundongos , Camundongos Knockout para ApoE , NF-kappa B/metabolismo , Distribuição Aleatória , Ratos , Comprimidos
13.
Phys Chem Chem Phys ; 17(5): 3115-22, 2015 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-25516239

RESUMO

Current rectification is found in oxygen-substituted zigzag graphyne nanoribbon/hydrogen-terminated zigzag graphene nanoribbon heterostructure junctions, from the application of nonequilibrium Green's function formalism combined with density functional theory. This behavior could be tuned by varying the number and location of oxygen atoms in the zigzag graphyne nanoribbon parts, and the rectification direction could be reversed due to the parity limitation tunneling effect. Moreover, an obvious negative differential resistance behavior is found and may be explained by two different mechanisms.

14.
Artigo em Inglês | MEDLINE | ID: mdl-25530775

RESUMO

You Gui Wan (YGW) is a classic herbal formula in traditional Chinese medicine (TCM) used for the clinical treatment of infertility. This study was to explore whether YGW has an impact on mouse oocyte maturation in vitro and subsequent fertilization competence. Rat medicated serum containing YGW was prepared by orally administrating YGW. Mouse immature oocytes were cultured with YGW medicated serum and compared to those cultured with or without normal rat serum or follicle-stimulating hormone (FSH). YGW medicated serum significantly increased the percentages of matured oocytes when compared to the groups with or without normal rat serum (P < 0.01). Furthermore, YGW medicated serum increased the rate of in vitro fertilization (IVF) when compared to the groups treated with FSH and with or without normal rat serum (P < 0.001). YGW medicated serum also had significant effects on the mRNA expressions of PKA, CREB, MAPK, PKC, PKG, and MPF and the concentrations of cAMP, cGMP, and NO in matured oocytes. These results indicate that YGW can promote mouse oocyte maturation and IVF in vitro. Signaling pathways, such as the cAMP/PKA/MAPK, the PKC-MAPK, and the NO-cGMP-PKG pathway, which are similar to those induced by FSH, may be responsible for this action.

15.
Int J Gynecol Cancer ; 24(4): 643-8, 2014 May.
Artigo em Inglês | MEDLINE | ID: mdl-24662128

RESUMO

OBJECTIVE: Biopsy confirmed that cervical intraepithelial neoplasia (CIN) may naturally regress or progress. Currently, the risk assessment for CIN progression to cervical cancer is still not satisfactory in clinical practice. We investigated copy number and protein expression of TP63 and MYC and explored the possibility to use them as progression biomarkers. METHODS: Copy numbers of TP63 and MYC, as well as human papilloma virus (HPV) integration status, were determined by fluorescence in situ hybridization in 39 patients with CIN and 66 patients with cervical cancer. Corresponding protein expressions were analyzed by immunohistochemistry. Receiver operating characteristic curves were used to measure the diagnostic test performance for the detection of cervical cancer from CIN. Sensitivity and specificity values of biomarkers were calculated. RESULTS: The average copy number and expression of TP63 and MYC, as well as the HPV integration rate, increased in the progression of CIN to cervical cancer. Receiver operating characteristic analysis for detection of cervical cancer resulted in area under the curve (AUC) values of TP63 copy number (AUC, 0.96; 95% confidence interval [CI], 0.91-1.00), MYC copy number (AUC, 0.92; 95% CI, 0.85-0.96), TP63 expression (AUC, 0.73; 95% CI, 0.61-0.85), and HPV-16 integration (AUC, 0.73; 95% CI, 0.60-0.85). MYC expression was not able to statistically distinguish cancer from CIN (P = 0.393). The combinations increased the specificity slightly but not sensitivity. Among them, TP63 amplification showed the best diagnostic performance. CONCLUSIONS: Amplification and overexpression of TP63 and MYC, and HPV integration rate, are associated with the transition of CIN to cervical cancer. Future studies on these biomarkers will help to assess the risk of CIN progression.


Assuntos
Biomarcadores Tumorais/metabolismo , Carcinoma de Células Escamosas/metabolismo , Neoplasia Intraepitelial Cervical/metabolismo , Amplificação de Genes , Proteínas Proto-Oncogênicas c-myc/metabolismo , Fatores de Transcrição/metabolismo , Proteínas Supressoras de Tumor/metabolismo , Neoplasias do Colo do Útero/metabolismo , Biomarcadores Tumorais/genética , Carcinoma de Células Escamosas/patologia , Carcinoma de Células Escamosas/cirurgia , Neoplasia Intraepitelial Cervical/patologia , Neoplasia Intraepitelial Cervical/cirurgia , Feminino , Seguimentos , Humanos , Técnicas Imunoenzimáticas , Hibridização in Situ Fluorescente , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Prognóstico , Proteínas Proto-Oncogênicas c-myc/genética , Curva ROC , Fatores de Transcrição/genética , Proteínas Supressoras de Tumor/genética , Neoplasias do Colo do Útero/patologia , Neoplasias do Colo do Útero/cirurgia
16.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 43(3): 348-51, 2012 May.
Artigo em Chinês | MEDLINE | ID: mdl-22812235

RESUMO

OBJECTIVE: To study the effects of activin A (ACTA) and inhibin A (INHA) on the in vitro maturation (IVM) of mice immature eggs and early embryonic development. METHODS: 1. The mice oocytes were cultured in the culture medium contained different concentration of ACTA and INHA (50 ng/mL, 100 ng/mL, 200 ng/mL) to find the optimum concentration; 2. The effects of different concentration of ACTA, INHA, ACTA+ INHA on the mature rates of mice oocytes were compared; 3. The effects of different concentration of ACTA, INHA, ACTA+INHA on the fertility rates and blastocysts formation rates of IVM mice eggs were also compared. RESULTS: The mature rates of mice oocytes in culture medium contained (100 ng/mL and 200 ng/mL) ACTA and INHA were significantly higher than that contained 50 ng/mL ACTA and INHA (P < 0.05); There was no difference between 100 ng/mL and 200 ng/mL groups. The mature rates, fertility rates and blastocyst rates of mice oocytes were significantly higher than those of control (P < 0.05). CONCLUSION: The mature rates, fertility rates and embryonic development potential after fertilization of mice oocytes were significantly promoted by ACTA, INHA. The optimum concentration of ACTA and INHA in culture medium was 100 ng/mL.


Assuntos
Ativinas/farmacologia , Técnicas de Maturação in Vitro de Oócitos/métodos , Inibinas/farmacologia , Oócitos/efeitos dos fármacos , Animais , Células Cultivadas , Feminino , Fertilização In Vitro , Masculino , Camundongos , Oócitos/citologia
17.
Artigo em Inglês | MEDLINE | ID: mdl-22484254

RESUMO

The interactions of rhodium complex RhCl(CO)(TPPTS)(2) [TPPTS=P(m-C(6)H(4)SO(3)Na)(3)] with cationic, nonionic, and anionic surfactants have been investigated by UV-vis, fluorescence and (1)H NMR measurements. The presence of four different species of RhCl(CO)(TPPTS)(2) in cationic cetyltrimethylammonium (CTAB) solution has been demonstrated: free rhodium complex, rhodium complex bound to CTAB monomer, rhodium complex bound to CTAB premicelles, rhodium complex bound to CTAB micelles. The spectroscopy data show that RhCl(CO)(TPPTS)(2) can adsorb on the interface of cationic CTAB micelles by strong electrostatic attraction, weakly bind to the nonionic polyoxyethylene (20) sorbitan monolaurate (Tween 20) micelles by hydrophobic interaction, and does not interact with anion sodium dodecyl sulfate (SDS) micelles due to the strong electrostatic repulsion.


Assuntos
Complexos de Coordenação/química , Ródio/química , Tensoativos/química , Cetrimônio , Compostos de Cetrimônio/química , Espectroscopia de Ressonância Magnética , Micelas , Polissorbatos/química , Dodecilsulfato de Sódio/química , Solubilidade , Espectrometria de Fluorescência , Água/química
18.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 42(6): 789-91, 796, 2011 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-22332543

RESUMO

OBJECTIVE: To study the influence of Compound Chinese medicine for reinforcing kidney on sperm fertilizing ability of mice. METHODS: Twenty male mice were randomly divided into two groups. The mice in treatment groups were given Compound Chinese medicine for reinforcing kidney every day for 14 d, and the mice in control group were given saline. Seven days after the treatments, the male mice mated with superovulated female mice. After the successful mating, the female mice were executed, then 1 cell embryos and fertilized eggs were harvested from oviduct. The embryos cleavage rate and the percentage rate of blastocysts were observed in vivo. In vitro fertilization (IVF%), the percentage of blastocysts, the percentage of acrosome-intact spermatozoa (AIS%) and the acrosin activity were studied in vitro. RESULTS: Compared with the controls, the mice receiving Compound Chinese medicine had higher embryos cleavage rate and IVF%. The percentage of blastocysts between the two groups was no difference. The acrosin activity of treated mice was significant higher than that of control mcie, and AIS% between the two groups was no significant different. CONCLUSION: Compound Chinese medicine for reinforcing kidney can improve the mouse sperm fertilizing ability, which may be relevant to acrosin activity.


Assuntos
Acrosina/metabolismo , Medicamentos de Ervas Chinesas/farmacologia , Fertilização/efeitos dos fármacos , Motilidade Espermática/efeitos dos fármacos , Espermatozoides/efeitos dos fármacos , Animais , Blastocisto/efeitos dos fármacos , Feminino , Fertilização In Vitro , Masculino , Camundongos , Camundongos Endogâmicos ICR , Distribuição Aleatória , Espermatozoides/fisiologia
19.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 41(5): 810-3, 2010 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-21302447

RESUMO

OBJECTIVE: To study the ultrastructure of unfertilized human oocytes and undivided human zygoytes. METHODS: Unfertilized human oocytes and undivided human zygoytes were collected during in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI), and then observed under transmission electron microscope (TEM) after fixation, dehydration, embedding, and ultrashing. RESULTS: The unfertilized oocytes from conventional IVF showed (1) normal zona pellucida (ZP) but without cortical granules (CG) beneath oolemma, condensed sperm nuclei, and disappearance of mitochondria and smooth endoplasmic reticulum (SER), (2) abnormal dense in ZP, lack of other organelles [SER tubuli (SER-T),SER vesicles (SER-V)] besides CG and mitochondria, and intact acrosome that penetrated the ZP. The unfertilized oocytes from ICSI showed (1) normal cortex containing one row of dense cortical granules beneath oolemma, SER-T and SER-V associated with mitochondria, some injected spermatozoon with lost nuclear envelope, and some decondensed chromatin, (2) little or scattered cortical granules, which dispersed beneath oolemma, scarce mitochondria and SER-T or isolated mitochondria. The undivided zygotes showed cortical granules contents free in the perivitelline space, little mitochondria, some second lysosomes filled with droplets, and large clumps of annulate lamellae (AL). CONCLUSION: Appearance of organelles in abnormal position is related to oocytes cytoplasmic maturation, which leads to failure in fertilization. Absence of aerosome reaction and abnormal dense of zona pellucida are associated with failed fertilization in conventional IVF. Absence of oocyte activation is associated with failed fertilization in ICSI. The presence of lipofuscin body and abnormal assembly of annulate lamellae are associated with fertilization arrest at the pronuclear stage of human zygotic development.


Assuntos
Fertilização In Vitro , Oócitos/ultraestrutura , Zigoto/ultraestrutura , Feminino , Fertilização , Humanos , Microscopia Eletrônica de Transmissão , Injeções de Esperma Intracitoplásmicas , Zona Pelúcida/ultraestrutura
20.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 39(6): 929-32, 2008 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-19253828

RESUMO

OBJECTIVE: To study the influence of circadian Clock gene on the fertilization ability of sperm via attenuating the expression of Clock with RNAi in male mice. METHODS: After the injection of RNAi plasmid into mice testes, the expression of circadian Clock gene of testis tissue was determined by western Blotting. The fertilization ability of sperm was evaluated by various indices, including litter size in mated female mice, sperm count, sperm motility, in vitro fertilization (IVF) rate and acrosome development. RESULTS: The RNAi plasmid targeting circadian gene Clock attenuated the expression of CLOCK in mice testis, reduced in vitro fertilization CIVF) rate and the conception rate in viva, and also decreased sperm acrosin activity. CONCLUSION: Circadian gene Clock is related to the reproductive function in male mice. It probably affect sperm fertilization ability by regulating sperm acrosin activity.


Assuntos
Proteínas CLOCK/genética , Fertilidade/genética , Interferência de RNA , RNA Interferente Pequeno/genética , Capacitação Espermática/genética , Animais , Proteínas CLOCK/metabolismo , Fertilização In Vitro , Masculino , Camundongos
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