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1.
Transfusion ; 2021 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-34813663

RESUMO

BACKGROUND: Cold agglutinin disease (CAD) is a rare autoimmune hemolytic anemia mediated by immunoglobulin M autoantibodies that bind to the "I" antigen on erythrocytes. IgM binding results in either agglutination at ≤37°C, activation of the classical complement pathway, or both. Patients with CAD can have transient agglutination-mediated circulatory symptoms triggered by exposure to cold conditions. Separately, patients with CAD can experience complement-mediated symptoms such as anemia, hemolysis, and fatigue, but the effect of the season on these complement-mediated manifestations of CAD and clinical outcomes is not well understood. METHODS: Using data from the Optum® de-identified Electronic Health Record dataset, we compared hemoglobin, markers of hemolysis (bilirubin and lactate dehydrogenase [LDH]), and healthcare resource utilization (HRU) between seasons for 594 patients (62% female; 66% aged ≥65 years) with CAD (defined as having CAD-related terms in their clinical notes on ≥3 separate occasions between December 2008 and May 2016). Laboratory parameters and HRU were compared between seasons using multivariate regression models. RESULTS: Estimated median hemoglobin (9.87 g/dL in summer and 9.86 g/dL in winter; P = 0.944) and bilirubin (1.04 mg/dL in summer and 1.09 mg/dL in winter; P = 0.257) were similar in winter versus summer. While LDH was statistically significantly higher in winter compared with summer (P < 0.001), the estimated median value was above normal for both seasons (309 U/L in summer and 367 U/L in winter). HRU measures and transfusion and thromboembolism rates were similar across seasons. CONCLUSIONS: Patients with CAD had evidence of persistent chronic hemolysis, HRU, and thromboembolism risk year round.

2.
Gastroenterol Res Pract ; 2021: 9699499, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34764993

RESUMO

Background: In recent years, the incidence of colorectal cancer (CRC) has continued to increase. Although the overall prognosis of CRC has improved with the continuous improvement of the level of treatment, the prognosis of metastatic colorectal cancer (mCRC) is still poor. The purpose of our study is to explore the prognostic value of platelet to lymphocyte ratio (PLR) in mCRC. Methods: The PubMed, Web of Science, and Embase (via OVID) were systematically searched to obtain all relevant research. We used hazard ratio (HR) with 95% confidence interval (CI) to assess the associations of PLR and overall survival (OS) and progression free survival (PFS). Results: A total of twelve studies containing 1452 patients were included in this meta-analysis. Pooled analysis showed that high levels of PLR were associated with poor OS (HR: 1.72, 95% CI: 1.27-2.33, and P < 0.01) and PFS (HR: 1.64, 95% CI: 1.16-2.31, and P = 0.033). Conclusion: Our analysis suggested that high levels of PLR pretreatment may be an effective predictive biomarker for the prognosis of mCRC patients.

3.
Plant Dis ; : PDIS02210396RE, 2021 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-34743537

RESUMO

Pine wilt disease is the most devastating pine disease caused by Bursaphelenchus xylophilus. Bursaphelenchus mucronatus is morphologically similar to B. xylophilus and geographically overlaps in its distribution. Although interspecific hybridization of the two nematodes has been performed in vitro, the dynamic regularity of hybrid formation and its risk in forests has not been well evaluated. In this study, a hybrid of B. xylophilus and Bursaphelenchus mucronatus mucronatus was identified in the laboratory and fields by molecular markers. The heterozygosity of ITS-5.8S loci for identification was unstable in the hybrid population, and the allele inherited from B. m. mucronatus was lost over several generations. We also provided evidence that hybrids existed in some new epidemic areas, while old epidemic areas were usually dominated by B. xylophilus. Hybrids could be generated when B. m. mucronatus was invaded by B. xylophilus, and the pathogenicity of the hybrids was similar to that of B. xylophilus. These findings may improve the understanding of the natural hybridization between B. xylophilus and B. m. mucronatus and pathogenic variation in pine wilt disease, providing new insights for future studies on disease detection, transmission, and quarantine.

4.
Chemosphere ; 288(Pt 1): 132509, 2021 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-34627811

RESUMO

Herein, polypyrrole/titanium oxide/reduced graphene oxide (PTi/r-GO) electrodes were prepared and successfully applied for the photoelectrocatalytic (PEC) degradation of methyl orange (MO) under visible light. Polypyrrole-TiO2 composites rich in p-n heterojunctions were first prepared, then modified with r-GO to improve the electrical conductivity and facilitate charge separation under visible light irradiation. The obtained PTi/r-GO composites were then deposited onto a titanium mesh, which served as the working electrode in PEC experiments. A MO removal efficiency of 93% was achieved in 50 min using PTi/r-GO electrode under PEC conditions (Xe lamp, λ > 420 nm, bias of 0.6 V, 0.1 M Na2SO4 electrolyte), which was far higher than MO removal efficiencies under electrocatalytic oxidation (22%) or photocatalytic oxidation (47%) conditions. This confirmed that excellent activity of the PTi/r-GO electrode under PEC conditions was due to a combination of electrochemical and photocatalytic oxidation processes (involving •OH and •O2- generation). Further, PTi/r-GO was very stable under the applied PEC conditions, with the MO removal efficiency remaining >90% after five cycles. PEC degradation pathways for MO on PTi/r-GO were explored, with a number of key intermediates in the MO mineralization process identified. Results demonstrate that PEC electrodes combining p-type polypyrrole, n-type TiO2 and rGO are very effective in the treatment of hazardous organic compounds in wastewater.

5.
Mikrochim Acta ; 188(11): 393, 2021 Oct 26.
Artigo em Inglês | MEDLINE | ID: mdl-34698939

RESUMO

Solid contact-based ion-selective electrodes (SC-ISEs) based on silver nanoparticles/polyaniline (Ag@PANI) as the solid contact (SC) were successfully prepared. The Ag@PANI SC showed high capacitance and excellent electron transport performance. Owing to the synergetic effects of the Ag nanoparticles and PANI, a GC/Ag@PANI-II/Pb2+-ISE (where II refers to a Ag content of 0.01 wt% in the SC layer) showed a low Pb2+ detection limit (6.31 × 10-10 M) with a slope of 29.1 ± 0.3 mV/dec, a fast response (< 5 s), and high stability. GC/Ag@PANI-II/Pb2+-ISE exhibited a Nernstian response for Pb2+ ions over a wide concentration range (10-3 to 10-9 M). After a 3-week operation, GC/Ag@PANI-II/Pb2+-ISE responded linearly to Pb2+ over the range of 10-7-10-3 M, demonstrating good long-term potential stability. Furthermore, the electrode showed excellent reproducibility and repeatability of the potential values and was successfully applied to detect the Pb2+ concentration in real samples with a recovery of 97 - 109%. Results suggest that Ag@PANI composites offer good transducer performance in trace ion detection sensors.

6.
Spectrochim Acta A Mol Biomol Spectrosc ; 267(Pt 1): 120509, 2021 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-34688060

RESUMO

A water-soluble, stable, simple and dual ligands (bovine serum albumin and L-histidine)-enhanced copper nanoclusters (BSA-CuNCs@L-His) was synthesized by one-step wet chemical method. Interestingly, the introduction of L-His ligand could improve evidently the quantum yields (QYs, 3.47%) and stability of BSA-CuNCs due to forming the stronger interaction of L-His and Cu and producing bigger diameter CuNCs by coordination-induced aggregation. Thus, a new ratiometric fluorescent probe (RF-probe) was successfully exploited for sensitively and selectively mensurating doxycycline (DOX) because DOX could simultaneously regulate the fluorescence (FL) intensities of BSA-CuNCs@L-His at 410 and 520 nm. The FL quenching of BSA-CuNCs@L-His at 410 nm by DOX was mainly originated from the static quenching process, while DOX could bind to Trp-212 in BSA from the skeleton of BSA-CuNCs@L-His by electrostatic interaction causing the appearance of new emission peak at 520 nm. The content of DOX was monitored by the RF-probe with a linear range of 0.05-14.0 µM and a LOD (limit of detection) and LOQ (limit of quantification) of 6.4 and 21.3 nM (at 3σ/slope and 10σ/slope). Moreover, compared to the standard HPLC method, the proposed RF-probe was extended to the detection of DOX in doxycycline hydrochloride (DOXH) tablets, DOXH injections and DOXH capsules with satisfactory results.

7.
Plant J ; 2021 Oct 26.
Artigo em Inglês | MEDLINE | ID: mdl-34699639

RESUMO

Horticultural crops mainly include fruits, vegetables, ornamental trees and flowers, and tea trees (Melaleuca alternifolia). They produce a variety of nutrients for the daily human diet in addition to the nutrition provided by staple crops, and some of them additionally possess ornamental and medicinal features. As such, horticultural crops make unique and important contributions to both food security and a colorful lifestyle. Under the current climate change scenario, the growing population and limited arable land means that agriculture, and especially horticulture, has been facing unprecedented challenges to meet the diverse demands of human daily life. Breeding horticultural crops with high quality and adaptability and establishing an effective system that combines cultivation, post-harvest handling, and sales becomes increasingly imperative for horticultural production. This review discusses characteristic and recent research highlights in horticultural crops, focusing on the breeding of quality traits and the mechanisms that underpin them. It additionally addresses challenges and potential solutions in horticultural production and post-harvest practices. Finally, we provide a prospective as to how emerging technologies can be implemented alongside interdisciplinary basic research to enhance our understanding and exploitation of horticultural crops.

8.
Talanta ; 234: 122607, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34364420

RESUMO

By simultaneously regulating the photoluminescence of alloy Au/Ag nanoclusters (NCs) and thiamine (VB1) through MnO2 nanosheets (MnO2 NS), a novel ratiometric fluorescent probe (RF-probe) was established for sensitively and selectively monitoring proanthocyanidins (PAs). The introduction of Ag (I) ions could enhance significantly the quantum yields (QYs, 11.1%) of AuNCs based on the synthetic method of UVI (UV irradiation) combined with MWH (microwave heating). MnO2 NS could quench the fluorescence (FL) of Au/AgNCs mainly coming from Förster resonance energy transfer (FRET), while it could act as a nanozyme catalyst for directly catalyzing the oxidation of VB1 to produce highly fluorescent oxVB1. In the presence of PAs, MnO2 was reduced to Mn2+, which caused that its quenching capacity and oxidase-like activity were vanished, thus the FL of oxVB1 and Au/AgNCs was reduced and recovered. The concentration of PAs could be monitored by the RF-probe with a linear range of 0.27-22.4 µmol L-1 and corresponding limit of detection (LOD) and limit of quantification (LOQ) were calculated to be 75.9 and 250.5 nmol L-1. Furthermore, the RF-probe was successfully used for the determination of PAs in mineral water, PAs additive and PAs capsule with satisfactory results compared to the standard HPLC method.


Assuntos
Compostos de Manganês , Proantocianidinas , Corantes Fluorescentes , Limite de Detecção , Óxidos , Tiamina
9.
Cell Death Discov ; 7(1): 224, 2021 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-34455417

RESUMO

Hepatocellular carcinoma (HCC) is a heterogeneous tumor with an increased incidence worldwide accompanied by high mortality and dismal prognosis. Emerging evidence indicates that mesenchymal stem cells (MSCs)-derived exosomes possess protective effects against various human diseases by transporting microRNAs (miRNAs or miRs). We aimed to explore the role of exosomal miR-15a derived from MSCs and its related mechanisms in HCC. Exosomes were isolated from transduced MSCs and co-incubated with Hep3B and Huh7 cells. miR-15a expression was examined by RT-qPCR in HCC cells, MSCs, and secreted exosomes. CCK-8, transwell, and flow cytometry were used to detect the effects of miR-15a or spalt-like transcription factor 4 (SALL4) on cell proliferative, migrating, invasive, and apoptotic properties. A dual-luciferase reporter gene assay was performed to validate the predicted targeting relationship of miR-15a with SALL4. Finally, in vivo experiments in nude mice were implemented to assess the impact of exosome-delivered miR-15a on HCC. The exosomes from MSCs restrained HCC cell proliferative, migrating, and invasive potentials, and accelerated their apoptosis. miR-15a was expressed at low levels in HCC cells and could bind to SALL4, thus curtailing the proliferative, migrating, and invasive abilities of HCC cells. Exosomes successfully delivered miR-15a to HCC cells. Exosomal miR-15a depressed tumorigenicity and metastasis of HCC tumors in vivo. Overall, exosomal miR-15a from MSCs can downregulate SALL4 expression and thereby retard HCC development.

10.
Spectrochim Acta A Mol Biomol Spectrosc ; 263: 120194, 2021 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-34303220

RESUMO

A stable, water-soluble, heightened quantum yields (QYs) Au nanoclusters by the alliance between doping Ag and dual ligands (thiosalicylic acid and bovine serum albumin) (TSA/BSA-Au/AgNCs) was prepared using one-step wet chemical synthesis. The effect of different types of aromatic thiols and the molar ratio of Au-Ag on the photo-luminescence performance of AuNCs was discussed in detail. The alloy NCs is shown to be viable fluorescent method for vitamin B12 (VB12) and chlortetracycline hydrochloride (CCH) assays, and become an excellent temperature sensor in the range of 10-50 °C. The fluorescence (FL) of TSA/BSA-Au/AgNCs was quenched with the addition of VB12 or CCH coming from Förster resonance energy transfer (FRET) combined with inner filter effect (IFE). The method can detect VB12/CCH by fluorometry with a linear response in the range of 0.33-60.0/0.33-60.0 µmol·L-1 and a 71.0/64.0 nmol·L-1 detection limit (at 3σ/slope). Furthermore, the proposed method was extended to the assays of VB12 in mineral water or tablets and CCH in veterinary drug or ointment with satisfactory results.


Assuntos
Clortetraciclina , Nanopartículas Metálicas , Corantes Fluorescentes , Ouro , Ligantes , Soroalbumina Bovina , Espectrometria de Fluorescência , Vitamina B 12
11.
Cells ; 10(7)2021 06 25.
Artigo em Inglês | MEDLINE | ID: mdl-34202084

RESUMO

Male infertility is a multifactorial disease with a strong genetic background. Abnormal sperm morphologies have been found to be closely related to male infertility. Here, we conducted whole-exome sequencing in a cohort of 150 Han Chinese men with asthenoteratozoospermia. Two novel hemizygous mutations were identified in USP26, an X-linked gene preferentially expressed in the testis and encoding a deubiquitinating enzyme. These USP26 variants are extremely rare in human population genome databases and have been predicted to be deleterious by multiple bioinformatics tools. Hematoxylin-eosin staining and electron microscopy analyses of the spermatozoa from men harboring hemizygous USP26 variants showed a highly aberrant morphology and ultrastructure of the sperm heads and flagella. Real-time quantitative PCR and immunoblotting assays revealed obviously reduced levels of USP26 mRNA and protein in the spermatozoa from men harboring hemizygous deleterious variants of USP26. Furthermore, intracytoplasmic sperm injections performed on infertile men harboring hemizygous USP26 variants achieved satisfactory outcomes. Overall, our study demonstrates that USP26 is essential for normal sperm morphogenesis, and hemizygous USP26 mutations can induce X-linked asthenoteratozoospermia. These findings will provide effective guidance for the genetic and reproductive counseling of infertile men with asthenoteratozoospermia.


Assuntos
Astenozoospermia/genética , Cisteína Endopeptidases/genética , Mutação/genética , Grupo com Ancestrais do Continente Asiático/genética , Sequência de Bases , Cisteína Endopeptidases/metabolismo , Feminino , Heterozigoto , Humanos , Masculino , Modelos Moleculares , Mutação de Sentido Incorreto/genética , Linhagem , Fenótipo , Prognóstico , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Injeções de Esperma Intracitoplásmicas , Espermatozoides/metabolismo , Espermatozoides/patologia , Espermatozoides/ultraestrutura
12.
Langmuir ; 37(25): 7668-7680, 2021 Jun 29.
Artigo em Inglês | MEDLINE | ID: mdl-34126011

RESUMO

Polycationic Pp-x@g-C3N4 composite was synthesized through an in situ polymerization process of N-alkylpyridinium acetylenic alcohol bromide (p-x) above the surface of g-C3N4. The structure of p-0 and the Pp-x@g-C3N4 properties were checked by modern technologies. Photocatalytic tests of Pp-x@g-C3N4 in water splitting unveiled much better Pp-x@g-C3N4 hydrogen evolution activities by comparison with both g-C3N4 and Pp-0. The hydrogen production by Pp-0@g-C3N4 was 1654.5 µmol h-1 g-1, which is ∼26- and 22-fold greater in relation to what g-C3N4 and Pp-0 produced (62.7 and 75.0 µmol h-1 g-1, respectively), suggesting strong bilateral and synergistic interactions of g-C3N4 with Pp-0. Although the lengthening methylene chain in the polymers weakened the hydrogen generation ability of Pp-x@g-C3N4, the conjugated double bonds, solubilization, and dispersion of Pp-x polycationic surfactants made Pp-x@g-C3N4 superior to g-C3N4 in water splitting. Due to the readily available raw materials, a simple way of preparation (starting chemicals to p-0 to Pp-0@g-C3N4), high photocatalysis efficiency, light irritation stability, recyclable ability, and low toxicity, Pp-0@g-C3N4 is a good candidate for water splitting.

13.
J Med Internet Res ; 23(6): e24303, 2021 06 01.
Artigo em Inglês | MEDLINE | ID: mdl-34061035

RESUMO

BACKGROUND: Social network-based strategies can expand HIV/syphilis self-tests among men who have sex with men (MSM). Sexual health influencers are individuals who are particularly capable of spreading information about HIV and other sexually transmitted infections (STIs) within their social networks. However, it remains unknown whether a sexual health influencer can encourage their peers to self-test for HIV/syphilis. OBJECTIVE: The aims of this study were to examine the impact of MSM sexual health influencers on improving HIV/syphilis self-test uptake within their social networks compared to that of nonsexual health influencers. METHODS: In Zhuhai, China, men 16 years or older, born biologically male, who reported ever having had sex with a man, and applying for HIV/syphilis self-tests were enrolled online as indexes and encouraged to distribute self-tests to individuals (alters) in their social network. Indexes scoring >3 on a sexual health influencer scale were considered to be sexual health influencers (Cronbach α=.87). The primary outcome was the mean number of alters encouraged to test per index for sexual health influencers compared with the number encouraged by noninfluencers. RESULTS: Participants included 371 indexes and 278 alters. Among indexes, 77 (20.8%) were sexual health influencers and 294 (79.2%) were noninfluencers. On average, each sexual health influencer successfully encouraged 1.66 alters to self-test compared to 0.51 alters encouraged by each noninfluencer (adjusted rate ratio 2.07, 95% CI 1.59-2.69). More sexual health influencers disclosed their sexual orientation (80.5% vs 67.3%, P=.02) and were community-based organization volunteers (18.2% vs 2.7%, P<.001) than noninfluencers. More alters of sexual health influencers came from a rural area (45.5% vs 23.8%, P<.001), had below-college education (57.7% vs 37.1%, P<.001), and had multiple casual male sexual partners in the past 6 months (25.2% vs 11.9%, P<.001). CONCLUSIONS: Being a sexual health influencer was associated with encouraging more alters with less testing access to self-test for HIV/syphilis. Sexual health influencers can be engaged as seeds to expand HIV/syphilis testing coverage.


Assuntos
Infecções por HIV , Saúde Sexual , Minorias Sexuais e de Gênero , Sífilis , China/epidemiologia , Estudos Transversais , Feminino , Infecções por HIV/diagnóstico , Infecções por HIV/epidemiologia , Homossexualidade Masculina , Humanos , Masculino , Comportamento Sexual , Parceiros Sexuais , Inquéritos e Questionários , Sífilis/diagnóstico , Sífilis/epidemiologia
14.
Exp Eye Res ; 209: 108652, 2021 08.
Artigo em Inglês | MEDLINE | ID: mdl-34097904

RESUMO

Large animal model of optic nerve crush (ONC) plays an important role in translating novel therapeutic strategies developed in rodent model to clinical application. Due to the poor accessibility of the optic nerve (ON) in humans and large animals, lateral orbitotomy is needed to expose the retrobulbar ON. This study was to explore the effects of ONC and ON exposure with lateral orbitotomy (sham surgery) on the outer retinal function and structure in goats by using standard flash electroretinogram (FERG) and spectral-domain optical coherence tomography (SD-OCT). We found that ONC led to a transient reduction in FERG amplitudes at 1 week post injury (wpi), which recovered gradually over 2 months afterwards. Sham surgery alone also caused a similar pattern of amplitude reduction in FERG, although not as significantly as ONC did. Transient outer retinal thickening following ONC occurred at 4 wpi (when progressive thinning of the ganglion cell complex began), peaked at 8 wpi, then recovered gradually at 12 wpi. In contrast, outer retinal thickness remained unchanged statistically 3 months after sham surgery. Fundus fluorescein angiography showed that neither ONC nor ON exposure with lateral orbitotomy significantly caused any significant delay or absence of central retinal vascular filling. In summary, ONC with lateral orbitotomy affects outer retinal function and structure transiently.

15.
Regul Toxicol Pharmacol ; 124: 104969, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34089813

RESUMO

Hexavalent chromium [Cr(VI)] exists in the ambient air at low concentrations (average upperbound ~0.1 ng/m3) yet airborne concentrations typically exceed EPA's Regional Screening Level for residential exposure (0.012 ng/m3) and other similar benchmarks, which assume a mutagenic mode of action (MOA) and use low-dose linear risk assessment models. We reviewed Cr(VI) inhalation unit risk estimates developed by researchers and regulatory agencies for environmental and occupational exposures and the underlying epidemiologic data, updated a previously published MOA analysis, and conducted dose-response modeling of rodent carcinogenicity data to evaluate the need for alternative exposure-response data and risk assessment approaches. Current research supports the role of non-mutagenic key events in the MOA, with growing evidence for epigenetic modifiers. Animal data show a weak carcinogenic response, even at cytotoxic exposures, and highlight the uncertainties associated with the current epidemiological data used in risk assessment. Points of departure from occupational and animal studies were used to determine margins of exposure (MOEs). MOEs range from 1.5 E+3 to 3.3 E+6 with a median of 5 E+5, indicating that current environmental exposures to Cr(VI) in ambient air should be considered of low concern. In this comprehensive review, the divergent results from default linear and MOE assessments support the need for more relevant and robust epidemiologic data, additional mechanistic studies, and refined risk assessment strategies.

16.
J Mater Chem B ; 9(17): 3662-3665, 2021 05 05.
Artigo em Inglês | MEDLINE | ID: mdl-33870986

RESUMO

A dual-site controlled pH probe, which is composed of gold nanoparticles and modified with rhodamine and fluorescein derivatives, was applied to sensitively monitor intracellular pH changes in sperm. The pH probe revealed the intracellular pH of sperm under different conditions and demonstrated the lower pH in asthenozoospermia patients as compared to healthy individuals. Importantly, the pH probe can help screen for healthy sperm.


Assuntos
Astenozoospermia/metabolismo , Citoplasma/metabolismo , Espermatozoides/metabolismo , Citoplasma/química , Citoplasma/ultraestrutura , Corantes Fluorescentes/química , Ouro/química , Humanos , Concentração de Íons de Hidrogênio , Masculino , Nanopartículas Metálicas/química , Imagem Óptica , Rodaminas/química , Análise do Sêmen , Espermatozoides/química , Espermatozoides/ultraestrutura
17.
Cell Signal ; 84: 110025, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-33915247

RESUMO

Recent studies have emphasized microRNAs (miRs) as crucial regulators in the occurrence and development of pancreatic cancer that continues to be one of the deadliest malignancies with few effective therapies. The study aimed to investigate the functional role of miR-873 and its associated mechanism to unravel the biological characteristics of pancreatic cancer stem cells in tumor growth. The expression patterns of pleckstrin-2 (PLEK2) and miR-873 were detected in the pancreatic cancer tissues. Then to further investigate specific role of miR-873, the pancreatic cancer stem cells were treated with miR-873 mimic, PLEK2, small interfering RNA against PLEK2, LY294002 (inhibitor of phosphatidylinositol 3-kinase/protein kinase B [PI3K/AKT] pathway) to detect the relative gene expression as well as their effects on cell self-renewal, proliferation and apoptosis. Finally, the tumor formation in nude mice was measured to verify the preceding results in vivo. Pancreatic cancer tissues exhibited a decline of miR-873 expression and an enhancement of PLEK2 expression. miR-873 targeted PLEK2 and downregulated its expression, leading to inhibition of PI3K/AKT pathway. Overexpressed miR-873 or silenced PLEK2 inhibited the self-renewal and proliferation while promoting the apoptosis of pancreatic cancer stem cells. Tumor formation was inhibited by overexpressed miR-873 or silenced PLEK2 in nude mice. Overall, miR-873 can suppress the self-renewal and proliferation of pancreatic cancer stem cells by blocking PLEK2-dependent PI3K/AKT pathway. Hence, this study contributes to understanding the role of miR-873 in pancreatic cancer stem cells and its underlying molecular mechanisms to aid in the development of effective pancreatic cancer therapeutics.

18.
Int J Genomics ; 2021: 6670390, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33816607

RESUMO

Background: MicroRNAs (miRNAs) have been demonstrated to exhibit important regulatory roles in multiple malignancies, including hepatocellular carcinoma (HCC). hsa-miR-497-5p was reported to involve in cancer progression and poor prognosis in many kinds of tumors. However, the expression and its clinical significance of hsa-miR-497-5p in HCC remain unclear. Methods: In the present study, we investigated the expression of hsa-miR-497-5p in HCC and analyzed the correction of clinical features with prognosis. The expression levels of hsa-miR-497-5p and potential target genes were analyzed in HCC and adjacent noncancerous tissues using The Cancer Genome Atlas (TCGA) database and Gene Expression Omnibus (GEO) datasets. Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to analyze hsa-miR-497-5p levels in 328 HCC tissues and 30 paired adjacent noncancer tissues. Overall survival (OS) and progression-free survival (PFS) of patients with HCC were assessed using the Kaplan-Meier method and the log-rank test. Results: The hsa-miR-497-5p expression levels were decreased, and its target genes ACTG1, CSNK1D, PPP1CC, and BIRC5 were upregulated in HCC tissues compared with normal tissues. Lower levels of hsa-miR-497-5p expression and higher levels of the four target genes were significantly associated with higher tumor diameter. Moreover, patients with lower hsa-miR-497-5p expression and higher target genes levels had shorter OS. Conclusion: The expression levels of hsa-miR-497-5p may play an important regulatory role in HCC and are closely correlated with HCC progression and poor prognosis in patients. The hsa-miR-497-5p may be a specific therapeutic target for the treatment of HCC.

19.
Artigo em Inglês | MEDLINE | ID: mdl-33669199

RESUMO

Rapid economic and societal development increases resource consumption. Understanding how to balance the discrepancy between economic and social water use and ecological water use is an urgent problem to be solved, especially in arid areas. The Heihe River is the second-largest inland river in China, and this problem is notable. To ensure the downstream ecological water use, the "Water Distribution Plan for the Mainstream of the Heihe River" (97 Water Diversion Scheme) controls the discharge of Yingluo Gorge and Zhengyi Gorge, while the "Opinions of applying the strictest water resources control system" (Three Red Lines) restricts the water use. With the development of the economy and agriculture in the midstream, Zhengyi Gorge's discharge cannot reach the Heihe River's ecological water downstream. This paper is under the constraints of the "97 Water Diversion Scheme" of Heihe River and the "Three Red Lines" of the total water use control index for Zhangye County. We constructed a water resource allocation model for the midstream of Heihe River to reasonably allocate water resources in the Heihe River's midstream and downstream. This model is divided into three parts: Establish the mathematical equation, simulate the water consumption under the different inflow conditions, and ensure each water user's demand. The result showed that if we fail to confine total water consumption in the midstream, through the reasonable allocation of water resources, the real water use and water consumption of the middle Heihe River will be greater than the "97 Water Diversion Scheme" and the "Three Red Lines." If we confine water consumption, they will be within the "97 Water Diversion Scheme" and the "Three Red Lines," at the same time, they can reach the downstream of the Heihe River's ecological water. Besides, under the premise of satisfying the economic water and ecological water downstream of the Heihe River, returning farmland to wasteland and strengthening water-saving measures will improve water efficiency and be more conducive to allocating water resources.


Assuntos
Recursos Hídricos , Água , China , Alocação de Recursos , Rios
20.
Hortic Res ; 8(1): 42, 2021 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-33642595

RESUMO

The characteristic secondary metabolites in tea (theanine, caffeine, and catechins) are important factors contributing to unique tea flavors. However, there has been relatively little research on molecular markers related to these metabolites. Thus, we conducted a genome-wide association analysis of the levels of these tea flavor-related metabolites in three seasons. The theanine, caffeine, and catechin levels in Population 1 comprising 191 tea plant germplasms were examined, which revealed that their heritability exceeded 0.5 in the analyzed seasons, with the following rank order (highest to lowest heritabilities): (+)-catechin > (-)-gallocatechin gallate > caffeine = (-)-epicatechin > (-)-epigallocatechin-3-gallate > theanine > (-)-epigallocatechin > (-)-epicatechin-3-gallate > catechin gallate > (+)-gallocatechin. The SNPs detected by amplified-fragment SNP and methylation sequencing divided Population 1 into three groups and seven subgroups. An association analysis yielded 307 SNP markers related to theanine, caffeine, and catechins that were common to all three seasons. Some of the markers were pleiotropic. The functional annotation of 180 key genes at the SNP loci revealed that FLS, UGT, MYB, and WD40 domain-containing proteins, as well as ATP-binding cassette transporters, may be important for catechin synthesis. KEGG and GO analyses indicated that these genes are associated with metabolic pathways and secondary metabolite biosynthesis. Moreover, in Population 2 (98 tea plant germplasm resources), 30 candidate SNPs were verified, including 17 SNPs that were significantly or extremely significantly associated with specific metabolite levels. These results will provide a foundation for future research on important flavor-related metabolites and may help accelerate the breeding of new tea varieties.

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