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1.
BMC Psychiatry ; 19(1): 212, 2019 Jul 05.
Artigo em Inglês | MEDLINE | ID: mdl-31277613

RESUMO

BACKGROUND: Narcissistic personality disorder (NPD) has never been applied in Chinese clinical practice, and the distribution of NPD in the clinical population of China is largely unknown. The current study uses two-stage clinic-based screening to investigate the frequency and clinical features of NPD in a Chinese help-seeking sample. METHODS: A total of 1402 consecutive outpatients ages 18-60 were recruited during their visit to the Shanghai Mental Health Center and screened with the Personality Diagnostic Questionnaire Fourth Edition Plus (PDQ-4+) and Structured Clinical Interview for the Diagnostic and Statistical Manual of Mental Disorders (DSM-IV) Axis II (SCID-II). The structured clinical interview was administered to estimate the rate of NPD and the frequency of each disorder criterion. RESULTS: The frequency estimate of NPD in the total sample was 4.0%. Among the 56 outpatients who met the criteria for NPD, there were more males than females, and many had a better educational background. The SCID-II interviews revealed high frequencies of diagnostic criterion 1 ("exaggerated sense of self-importance. NPD likely overlaps with Histrionic PD, Borderline PD, and Paranoid PD. This two stage screening method can enhance detection of Chinese NPD patients in clinical settings. CONCLUSIONS: Narcissism pathology is not rare in the Chinese psychiatric community when using the DSM-IV NPD criteria. Existing evidence suggests, at least indirectly, that there are important benefits of NPD diagnosis in psychiatric practice.

2.
Chem Biol Interact ; 293: 94-99, 2018 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-30040915

RESUMO

BACKGROUND: MMP-1 (Matrix metalloproteinase-1) promotes carcinogenesis and distant metastasis in different cancers. Regulation of MMP-1 could occur at multiple levels: epigenetically, post-transcriptionally, or post-translationally. An increasing body of evidence supports that the cytoplasmic transcription factor STAT3 (signal transducer and activator of transcription 3) is activated constitutively in a variety of cancers wherein it significantly affects the growth of tumors and also facilitates metastasis. In addition, STAT3 has been found to regulate nuclear activity pro-inflammatory transcriptional factor, NF-κB signaling, especially, the alternative one (RelB/p100) by directly interacting with them METHOD AND RESULTS: In this proof of concept study, we tested the hypothesis that STAT3 interacts with RelB to promote tumor invasion by positively regulating MMP-1 in colon cancer. We found that RelB and STAT3 were constitutively localized in the nucleus of colon cancer in surgically-resected specimens with use of Western blot analysis, which was further confirmed by immunofluorescence (IF) staining in colon carcinoma cell line HT29. We further observed that STAT3/RelB knockdown resulted in reduced MMP-1. Our results from chromatin immunoprecipitation studies further established that association between RelB and MMP-1 promoter decreased when STAT3 was depleted, and conversely, STAT3 association with MMP-1 decreased with the knockdown of RelB. CONCLUSION: These results suggest that STAT3 and ReB constitute a minimal activator complex for positive regulation of MMP-1 in colon cancer.


Assuntos
Neoplasias do Colo/patologia , Metaloproteinase 1 da Matriz/metabolismo , Fator de Transcrição STAT3/metabolismo , Fator de Transcrição RelB/metabolismo , Neoplasias do Colo/metabolismo , Células HT29 , Humanos , Metaloproteinase 1 da Matriz/genética , Regiões Promotoras Genéticas , Interferência de RNA , RNA Interferente Pequeno/metabolismo , Fator de Transcrição STAT3/antagonistas & inibidores , Fator de Transcrição STAT3/genética , Fator de Transcrição RelA/antagonistas & inibidores , Fator de Transcrição RelA/genética , Fator de Transcrição RelA/metabolismo , Fator de Transcrição RelB/antagonistas & inibidores , Fator de Transcrição RelB/genética , Regulação para Cima
3.
Oncotarget ; 8(34): 56296-56310, 2017 Aug 22.
Artigo em Inglês | MEDLINE | ID: mdl-28915591

RESUMO

Eugenol can be separated from the oil extract of clove bud, and has many pharmacological functions such as anticancer and transdermal absorption. HER2/PI3K-AKT is a key signaling pathway in the development of breast cancer. In this study, 80 µM eugenol could significantly inhibit the proliferation of HER-2 positive MCF-10AT cells and the inhibition rate was up to 32.8%, but had no obvious inhibitory effect on MCF-7 and MCF-10A cells with HER2 weak expression. Eugenol also significantly induced human breast precancerous lesion MCF-10AT cell apoptosis and cell cycle S-phase arrest, but the biological effects nearly disappeared after HER2 over-expression through transfecting pcDNA3.1-HER2. In MCF-10AT cells treated by 180 µM eugenol, the protein expressions of HER2, AKT, PDK1, p85, Bcl2, NF-κB, Bad and Cyclin D1 were decreased and the decreased rates were respectively 63.0%, 60.0%, 52.9%, 62.9%, 37.1%, 47.2%, 61.7%, 59.1%, while the p21, p27 and Bax expression were increased by 4.48-, 4.76- and 2.57-fold respectively. In the rat models of breast precancerous lesion, 1 mg eugenol for external use significantly inhibited the progress of breast precancerous lesion and the occurrence rate of breast precancerous lesions and invasive carcinomas was decreased by about 30.5%. Furthermore eugenol for external (1 mg) markedly decreased the protein expressions of HER2 (62.9%), AKT (58.6%), PDK1 (56.4%), p85 (54.3%), Bcl2 (59.3%), NF-κB (65.7%), Bad (64.0%), Cyclin D1 (43.0%), while p21, p27 and Bax protein expressions were respectively increased 1.83-, 2.52- and 2.51-fold. The results showed eugenol could significantly inhibit the development of breast precancerous lesions by blocking HER2/PI3K-AKT signaling network. So eugenol may be a promising external drug for breast precancerous lesions.

4.
J Asian Nat Prod Res ; : 1-8, 2017 Sep 11.
Artigo em Inglês | MEDLINE | ID: mdl-28891305

RESUMO

Phytochemical investigation of the leaves of Armeniaca sibirica (L.) Lam. led to the isolation of two new phenolic acids (1-2), together with eight known compounds (3-10) from the ethanol extracts of this plant. Structures of these compounds were elucidated through detailed spectroscopic analyses, using 1D-NMR and 2D-NMR in combination with HR-EI-MS techniques. All the compounds were evaluated for their antioxidant capabilities in vitro using 2, 2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS), 1, 1'-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assays, and ferric-reducing antioxidant power (FRAP) methods.

5.
Chemosphere ; 184: 795-805, 2017 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-28645083

RESUMO

Graphene oxide (GO) has widespread concerns in the fields of biological sciences and medical applications. Currently, studies have reported that excessive GO exposure can cause cellular DNA damage through reactive oxygen species (ROS) generation. However, DNA damage mediated response of the base excision repair (BER) pathway due to GO exposure is not elucidated yet. Therefore, we exposed HEK293T cells and zebrafish embryos to different concentrations of GO for 24 h, and transcriptional profiles of BER pathway genes, DNA damage, and cell viability were analyzed both in vitro and in vivo. Moreover, the deformation of HEK293T cells before and after GO exposure was also investigated using atomic force microscopy (AFM) to identify the physical changes occurred in the cells' structure. CCK-8 and Comet assay revealed the significant decrease in cell viability and increase in DNA damage in HEK293T cells at higher GO doses (25 and 50 µg/mL). Among the investigated genetic markers in HEK293T cells, BER pathway genes (APEX1, OGG1, CREB1, UNG) were significantly up-regulated upon exposure to higher GO dose (50 µg/mL), however, low exposure concentration (5, 25 µg/mL) failed to induce significant genetic induction except for CREB1 at 25 µg/mL. Additionally, the viscosity of HEK293T cells decreased upon GO exposure. In zebrafish, the results of up-regulated gene expressions (apex1, ogg1, polb, creb1) were consistent with those in the HEK293T cells. Taken all together, the exposure to elevated GO concentration could cause DNA damage to HEK293T cells and zebrafish embryos; BER pathway could be proposed as the possible inner response mechanism.


Assuntos
Dano ao DNA , Grafite/toxicidade , Nanoestruturas/toxicidade , Animais , Sobrevivência Celular , Ensaio Cometa , DNA Glicosilases , Reparo do DNA , Embrião não Mamífero , Células HEK293 , Humanos , Óxidos/metabolismo , Transdução de Sinais , Regulação para Cima , Peixe-Zebra/metabolismo
6.
World J Gastroenterol ; 23(21): 3850-3863, 2017 Jun 07.
Artigo em Inglês | MEDLINE | ID: mdl-28638225

RESUMO

AIM: To assess the effect of sodium selenite on the severity of dextran sulfate sodium (DSS)-induced colitis in C57BL/6 mice. METHODS: Mice were randomly divided into four groups (n = 10/group): normal group, selenium (Se) group, chronic colitis group, and Se + chronic colitis group. The mice were sacrificed on day 26. Survival rates, clinical symptoms, colon length, and histological changes were determined. The percentages and absolute numbers of immune system cells in the lamina propria lymphocytes (LPL) of the colon, the expression of mRNA in colon tissue, and the concentrations of Th1, Th17, and Treg cytokines in LPL from the large intestine, were measured. RESULTS: Se significantly ameliorated the symptoms of colitis and histological injury (P < 0.05 each), increasing the proportions of neutrophils and CD4+ CD25+ T cells (P < 0.05 each) and decreasing the proportions of γδT cells, CD4+, CD4+CD44+, and CD4+ CD69+ T cells in LPL (P < 0.05 each). Moreover, Se reduced the expression of IL-6, IFN-γ, IL-17A, IL-21, T-bet, and RORγt (P < 0.05 each), but enhanced the expression of IL-10 and Foxp3 (P < 0.05 each). CONCLUSION: These results suggest that Se protects against DSS-induced chronic colitis perhaps by increasing the number of CD4(+)CD25(+) Tregs that suppress the secretion of proinflammatory cytokines and populations of Th1, Th17, and γδT cells.


Assuntos
Colite/tratamento farmacológico , Linfócitos Intraepiteliais/efeitos dos fármacos , Linfócitos T Reguladores/efeitos dos fármacos , Células Th1/efeitos dos fármacos , Células Th17/efeitos dos fármacos , Oligoelementos/farmacologia , Animais , Antioxidantes/farmacologia , Antioxidantes/uso terapêutico , Doença Crônica , Colite/induzido quimicamente , Colite/imunologia , Colo/efeitos dos fármacos , Colo/imunologia , Colo/patologia , Citocinas/metabolismo , Sulfato de Dextrana/toxicidade , Modelos Animais de Doenças , Citometria de Fluxo , Humanos , Mucosa Intestinal/efeitos dos fármacos , Mucosa Intestinal/imunologia , Mucosa Intestinal/patologia , Linfócitos Intraepiteliais/imunologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Selenito de Sódio/farmacologia , Selenito de Sódio/uso terapêutico , Linfócitos T Reguladores/imunologia , Linfócitos T Reguladores/metabolismo , Células Th1/imunologia , Células Th1/metabolismo , Células Th17/imunologia , Células Th17/metabolismo , Oligoelementos/uso terapêutico
7.
Mol Clin Oncol ; 6(4): 477-482, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-28413652

RESUMO

Ocular toxicity is an uncommon complication of cytotoxic chemotherapy. Bilateral blindness with secondary retinitis pigmentosa (RP) following docetaxel and platinum combination chemotherapy at the recommended dose is extremely rare. The present study reports a case of advanced small-cell carcinoma (SCC) of the endometrium in a patient with diabetes mellitus type 2. The patient suffered from RP with a sharp decline in vision after the fourth course of postoperative docetaxel and platinum combination chemotherapy. Unfortunately, the patient developed bilateral blindness after another course of chemotherapy at a reduced dose. No tumor recurrence was observed during the 33 months of follow-up. A total of 35 cases of docetaxel- and/or platinum-induced retinal toxicity were found in the English literature and reviewed. The ischemic and electrophysiological hypotheses may have been implicated in the pathogenesis of ocular toxicity in the present case, particularly with the history of diabetes. Understanding the ocular side effects of this combination chemotherapy may assist gynecological oncologists and ophthalmologists with early recognition and timely intervention before blindness is established.

8.
Clin Cancer Res ; 23(1): 214-224, 2017 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-27401250

RESUMO

PURPOSE: Lymphatic vessels are mainly regarded as passive conduits for the dissemination of cancer cells. In this study, we investigate whether and how the tumor-associated lymphatic vessels may play an active role in tumor metastasis. EXPERIMENTAL DESIGN: In situ laser capture microdissection of lymphatic vessels followed by cDNA microarray analysis was used to determine the expression profiling of lymphatic endothelial cells (LEC). Gene expression levels and activity of signaling pathways were measured by real-time RT-PCR, ELISA, or immunoblotting. Lymphangiogenesis was assessed by IHC. Lymph node metastasis was measured using fluorescence imaging. The effects of SEMA4C on lymphangiogenesis in vitro were evaluated using migration assay and tube-formation assay of LECs. RESULTS: Tumor-associated LECs are molecularly and functionally different from their normal counterparts. In addition to expressing high levels of membrane-bound SEMA4C, tumor-associated LECs also produced soluble SEMA4C (sSEMA4C). Increased serum sSEMA4C was detected in patients with breast cancer and cervical cancer. Patients with metastasis had much higher levels of serum sSEMA4C. sSEMA4C promoted lymphangiogenesis by activating PlexinB2-ERBB2 signaling in LECs, and promoted the proliferation and migration of tumor cells by activating PlexinB2-MET signaling, thus promoting lymphatic metastasis. Although the SEMA4C signaling pathways differ between LECs and tumor cells, RHOA activation was necessary for the effects of SEMA4C in both types of cells. CONCLUSIONS: Tumor-associated LECs produce sSEMA4C to promote lymphatic metastasis of tumors. Our results suggest that SEMA4C and RHOA might be potential therapeutic targets, and that higher serum sSEMA4C could be a marker for breast cancer and cervical cancer. Clin Cancer Res; 23(1); 214-24. ©2016 AACR.


Assuntos
Células Endoteliais/metabolismo , Regulação Neoplásica da Expressão Gênica , Neoplasias/genética , Neoplasias/metabolismo , Semaforinas/genética , Semaforinas/metabolismo , Animais , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células , Modelos Animais de Doenças , Feminino , Perfilação da Expressão Gênica , Xenoenxertos , Humanos , Imuno-Histoquímica , Linfangiogênese , Metástase Linfática , Vasos Linfáticos/metabolismo , Vasos Linfáticos/patologia , Camundongos Nus , Neoplasias/patologia , Receptor ErbB-2/metabolismo , Transdução de Sinais , Proteína rhoA de Ligação ao GTP/metabolismo
9.
Phys Rev E ; 94(2-2): 029902, 2016 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-27627430

RESUMO

This corrects the article DOI: 10.1103/PhysRevE.93.040201.

10.
Phys Rev E ; 93(4): 040201, 2016 04.
Artigo em Inglês | MEDLINE | ID: mdl-27176237

RESUMO

Distributions of eigenmodes are widely concerned in both bounded and open systems. In the realm of chaos, counting resonances can characterize the underlying dynamics (regular vs chaotic), and is often instrumental to identify classical-to-quantum correspondence. Here, we study, both theoretically and experimentally, the statistics of chaotic resonances in an optical microcavity with a mixed phase space of both regular and chaotic dynamics. Information on the number of chaotic modes is extracted by counting regular modes, which couple to the former via dynamical tunneling. The experimental data are in agreement with a known semiclassical prediction for the dependence of the number of chaotic resonances on the number of open channels, while they deviate significantly from a purely random-matrix-theory-based treatment, in general. We ascribe this result to the ballistic decay of the rays, which occurs within Ehrenfest time, and importantly, within the time scale of transient chaos. The present approach may provide a general tool for the statistical analysis of chaotic resonances in open systems.

11.
Oncotarget ; 7(52): 85750-85763, 2016 Dec 27.
Artigo em Inglês | MEDLINE | ID: mdl-26515590

RESUMO

Glia maturation factor-ß (GMF-ß) has been reported to promote glial differentiation, and act as a negative prognostic indicator in certain cancers. However, its roles in glioma progression remain unclear. Since neurogenesis and vasculogenesis were proved to share some common regulators during gliomagenesis, we aim to explore the potential impact of GMF-ß on tumor neovascularization and patient survival in glioma. In this study, we first detected GMF-ß expression not only in tumor cells but also in microvascular endothelia by double immunohistochemical staining. Both tumoral and endothelial GMF-ß expression levels were positively correlated with tumor grade and microvessel density (MVD), while negatively associated with poor prognoses of the patients. Interestingly, multivariate analysis demonstrated that endothelial GMF-ß expression level was the only independent predictor of progression-free and overall survival of glioma patients. The results of in vitro angiogenesis assay showed that GMF-ß knockdown significantly inhibited tubulogenesis of human U87 glioblastoma cells. Furthermore, GMF-ß knockdown suppressed tumor growth and the formation of human-CD31 positive (glioma cell-derived) microvessels in a mouse orthotopic U87 glioma model. Our results demonstrated that GMF-ß is an important player in glioma progression via promoting neovascularization. GMF-ß may therefore be a novel prognostic marker as well as a potential therapeutic target for glioma.


Assuntos
Neoplasias Encefálicas/irrigação sanguínea , Endotélio Vascular/fisiologia , Fator de Maturação da Glia/fisiologia , Glioma/irrigação sanguínea , Neovascularização Patológica/etiologia , Adulto , Idoso , Animais , Neoplasias Encefálicas/mortalidade , Neoplasias Encefálicas/patologia , Proliferação de Células , Feminino , Fator de Maturação da Glia/análise , Glioma/mortalidade , Glioma/patologia , Humanos , Masculino , Camundongos , Pessoa de Meia-Idade , Molécula-1 de Adesão Celular Endotelial a Plaquetas/análise
12.
World J Gastroenterol ; 21(9): 2862-4, 2015 Mar 07.
Artigo em Inglês | MEDLINE | ID: mdl-25759563

RESUMO

A 67-year-old man from Jingzhou was admitted to the First Hospital Affiliated to Yangtze University in July 2013 with sudden onset of abdominal pain with dizziness for 12 h. The patient had sign of peritoneal irritation. Ultrasonography of the abdomen and pelvis showed hepatic fibrosis due to schistosomiasis. Computed tomography showed free gas in the peritoneal cavity. Plain abdominal radiography showed bilateral subdiaphragmatic accumulation of gas, perforation of the viscus, and radio-opacity in the left renal area. The patient underwent emergency exploratory laparotomy. At laparotomy, a moderate amount of muddy yellow pus was found in the intra-abdominal cavity. At the junction of the jejunum and ileum, about 250 cm from Treitz's ligament, there was an about 10-cm length of inflamed small bowel with perforation (3 mm in diameter) along the mesenteric border at the middle of the lesion. The patient underwent resection of the affected intestinal segment, along with end-to-end intestinal anastomosis. Histopathological examination revealed mucosal necrosis and hemorrhage with a large number of infiltrating eosinophils and neutrophils, and acute submucosal inflammation with a large number of infiltrating eosinophils and neutrophils associated with Schistosoma japonicum (S. japonicum) eggs. No intravascular adult parasite was found. Postoperatively, the patient was treated with praziquantel (30 mg/kg daily) for 4 d. The patient progressed well. To the best of our knowledge, this is the first case of small bowel perforation associated with eggs of S. japonicum.


Assuntos
Enteropatias Parasitárias/parasitologia , Perfuração Intestinal/parasitologia , Intestino Delgado/parasitologia , Peritonite/parasitologia , Schistosoma japonicum/isolamento & purificação , Esquistossomose Japônica/parasitologia , Dor Abdominal/parasitologia , Idoso , Animais , Biópsia , Procedimentos Cirúrgicos do Sistema Digestório , Humanos , Enteropatias Parasitárias/diagnóstico , Enteropatias Parasitárias/terapia , Perfuração Intestinal/diagnóstico , Perfuração Intestinal/terapia , Intestino Delgado/cirurgia , Peritonite/diagnóstico , Peritonite/terapia , Esquistossomose Japônica/diagnóstico , Esquistossomose Japônica/terapia , Esquistossomicidas/uso terapêutico , Resultado do Tratamento
13.
Asian Pac J Cancer Prev ; 15(21): 9395-404, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25422231

RESUMO

BACKGROUND: Primary vaginal yolk sac tumor is a rare malignancy in the pediatric population, and a diagnostic challenge and appropriate initial treatment remains unsolved. The aim of this study was to investigate the clinicopathologic features, treatment and prognosis of this tumor. MATERIALS AND METHODS: Eight cases of primary vaginal yolk sac tumor were reported with a literature review. RESULTS: There were 4 pure yolk sac tumor cases and four mixed germ cell tumors containing yolk sac tumor element, including two cases with embryonal carcinoma and two cases with embryonal carcinoma and dysgerminoma. Partial vaginectomy was performed in four cases and all patients received chemotherapy. 85 cases in literatures were reviewed and 9 cases were misdiagnosed. Follow-up data was available in 77 cases and 5-year overall survival rate was 87.6%. 5-year survival rate of biopsy with chemotherapy, conservative surgery with chemotherapy and radical surgery with chemotherapy was 91.1%, 100% and 28.6%, respectively (p<0.001). Compared to cases without relapse or metastasis after initial treatment, patients with relapse or metastasis had a shorter overall survival (35.6% vs 96.6%, p<0.001). CONCLUSIONS: Mixed germ cell tumor containing yolk sac tumor element was not uncommon and partial vaginectomy may be a good choice for primary vaginal mixed yolk sac tumor type to eradicate local tumor cells and provide complete information for pathological diagnosis and postoperative adjuvant therapy.


Assuntos
Tumor do Seio Endodérmico/mortalidade , Tumor do Seio Endodérmico/patologia , Neoplasias Vaginais/mortalidade , Neoplasias Vaginais/patologia , China , Intervalo Livre de Doença , Tumor do Seio Endodérmico/cirurgia , Feminino , Humanos , Lactente , Invasividade Neoplásica/patologia , Estadiamento de Neoplasias , Prognóstico , Amostragem , Análise de Sobrevida , Resultado do Tratamento , Neoplasias Vaginais/cirurgia
14.
Adv Mater ; 26(44): 7462-7, 2014 Nov 26.
Artigo em Inglês | MEDLINE | ID: mdl-25164067

RESUMO

Single-nanoparticle detection and sizing is demonstrated using a nanofiber pair in an aqueous environment. The sizing of nanoparticles with a single radius (100 nm) and of mixed nanoparticles with different radii (100 nm and 170 nm) are both realized, and the experimental results agree well with predictions of Rayleigh-Gans scattering, by taking the inhomogeneous field distribution of the nanofibers into account.


Assuntos
Nanofibras/química , Nanopartículas/química , Tamanho da Partícula , Compostos de Ouro/química , Microscopia Eletrônica de Varredura , Modelos Químicos , Imagem Óptica , Água/química
15.
Asian Pac J Cancer Prev ; 15(2): 713-6, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24568484

RESUMO

OBJECTIVE: Connections between chronic inflammation and tumor development and progression are now generally accepted. Recent evidence indicates that hepatitis B is associated with several types of cancer, but whether endometrial carcinoma (EC) is included has not been reported. METHODS: We analyzed HBV serum marker status in 398 patients with endometrial cancer, comparing them to 788 control women undergoing health examination. RESULTS: The total prevalence of HBsAg tested positive in cancer group was significantly higher than the control group (12.8% vs 6.0%, P=0.001), while positive HBsAb was significantly lower (41.2% vs 68.5%, P=0.001). Hepatitis B carriers in endometrial cancer group were also more frequent than in the control group (9.3% vs 5.5%, P=0.013). Interestingly, in the endometrial cancer group, 147 cases were HBV serum marker negative, which was also higher than in the control group (36.9% vs 15.6%, P=0.001). CONCLUSION: There may be a correlation between HBV infection and endometrial carcinoma.


Assuntos
Neoplasias do Endométrio/epidemiologia , Neoplasias do Endométrio/virologia , Vírus da Hepatite B/patogenicidade , Hepatite B/complicações , Adulto , Estudos de Casos e Controles , China/epidemiologia , Neoplasias do Endométrio/sangue , Feminino , Seguimentos , Hepatite B/virologia , Anticorpos Anti-Hepatite B/sangue , Antígenos de Superfície da Hepatite B/sangue , Humanos , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Prevalência , Prognóstico
16.
Histol Histopathol ; 29(5): 567-87, 2014 May.
Artigo em Inglês | MEDLINE | ID: mdl-24327088

RESUMO

BACKGROUND: We analysed data on bacterial vaginosis (BV) contradicting the paradigm of mono-infection. METHODOLOGY: Tissues and epithelial cells of vagina, uterus, fallopian tubes and perianal region were investigated using fluorescence in situ hybridization (FISH) in women with BV and controls. RESULTS: Healthy vagina was free of biofilms. Prolific structured polymicrobial (StPM) Gardnerella-dominated biofilm characterised BV. The intact StPM-Gardnerella-biofilm enveloped desquamated vaginal/prepuce epithelial cells and was secreted with urine and sperma. The disease involved both genders and occurred in pairs. Children born to women with BV were negative. Monotherapy with metronidazole, moxifloxacin or local antiseptics suppressed but often did not eradicate StPM-Gardnerella-biofilms. There was no BV without Gardnerella, but Gardnerella was not BV. Outside of StPM-biofilm, Gardnerella was also found in a subset of children and healthy adults, but was dispersed, temporal and did not transform into StPM-Gardnerella-biofilm. CONCLUSIONS: StPM-Gardnerella-biofilm is an infectious subject. The assembly of single players to StPM-Gardnerella-biofilm is a not trivial every day process, but probably an evolutionary event with a long history of growth, propagation and selection for viability and ability to reshape the environment. The evolutionary memory is cemented in the structural differentiation of StPM-Gardnerella-biofilms and imparts them to resist previous and emerging challenges.


Assuntos
Biofilmes/crescimento & desenvolvimento , Gardnerella/patogenicidade , Infecções por Bactérias Gram-Positivas/microbiologia , Vaginose Bacteriana/microbiologia , Biofilmes/efeitos dos fármacos , Candidíase Vulvovaginal/microbiologia , Estudos de Casos e Controles , Feminino , Gardnerella/efeitos dos fármacos , Gardnerella/genética , Infecções por Bactérias Gram-Positivas/tratamento farmacológico , Humanos , Hibridização in Situ Fluorescente , Masculino , Gravidez , Vagina/microbiologia , Vaginose Bacteriana/tratamento farmacológico
17.
Adv Mater ; 25(39): 5616-20, 2013 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-24303524

RESUMO

A new label-free sensing mechanism is demonstrated experimentally by monitoring the whispering-gallery mode broadening in microcavities. It is immune to both noise from the probe laser and environmental disturbances, and is able to remove the strict requirement for ultra-high-Q mode cavities for sensitive nanoparticle detection. This ability to sense nanoscale objects and biological analytes is particularly crucial for wide applications.


Assuntos
Lentivirus/isolamento & purificação , Microtecnologia/métodos , Nanopartículas/análise
18.
Zhonghua Yi Xue Za Zhi ; 93(34): 2745-9, 2013 Sep 10.
Artigo em Chinês | MEDLINE | ID: mdl-24360113

RESUMO

OBJECTIVE: To explore the effects of B-cell specific Maloney leukemia virus integration site 1 (Bmi1) gene on endothelial cells promoting glioma stem cell (GSC)-like phenotype. METHODS: Glioblastoma cell line GL261 and brain micro-vessel endothelial cell line b.END3 were used. Transwell co-culture system, limit dilution assay, xenograft, real-time polymerase chain reaction (PCR), Western blot, fluorescence activating cell sorter (FACS) and gene knock-down assay were used to determine the GSC-like phenotype and Bmi1 gene expression in glioma cells. RESULTS: Compared with the control of GL261 cell alone, (1) more and larger tumor spheres formed after co-culturing with endothelial cells (62.5% ± 1.5% vs 25.0% ± 4.6% at 40 cells/well, P = 0.000). Xenografts generated by GL261 cells with b.END3 cells appeared earlier and were larger than that by GL261 cells alone ((0.798 ± 0.297) cm(3) vs (0.362 ± 0.123) cm(3), P = 0.000); (2) CD133 positive glioma cells increased after co-culturing with endothelial cells (8.48% ± 0.78% vs 4.81% ± 0.37%, P = 0.000); (3) the expression of Bmi1 in co-cultured glioma cells was up-regulated at mRNA level (2.72 ± 0.18 vs 1.00 ± 0.15, P = 0.000) and at protein level; (4) the above phenomenon was attenuated when Bmi1 gene expression was inhibited by siRNA in glioma cells, CD133 positive portion of Bmi1-knockdown GL261 cells co-culturing with b.END3 cells decreased than that of wildtype GL261 cells (0.34% ± 0.21% vs 1.70% ± 0.69%, P = 0.025). CONCLUSION: Endothelial cells promote GSC-like phenotype by up-regulating the expression of Bmi1 in glioma cells.


Assuntos
Células Endoteliais/citologia , Glioma/genética , Células-Tronco Neoplásicas/citologia , Complexo Repressor Polycomb 1/genética , Proteínas Proto-Oncogênicas/genética , Animais , Diferenciação Celular , Linhagem Celular Tumoral , Técnicas de Cocultura , Feminino , Camundongos , Camundongos Endogâmicos C57BL , Fenótipo
19.
Zhonghua Fu Chan Ke Za Zhi ; 48(8): 579-83, 2013 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-24199922

RESUMO

OBJECTIVE: To investigate the feasibility and effectiveness of human umbilical cord mesenchymal stem cells (HUCMSC) transplantation in the treatment of female stress urinary incontinence (SUI) in rats. METHODS: The 14 female SD rats of SUI model were established by vaginal balloon dilation after birth and maintain this status for four hours bilateral ovariectomy were performed after two weeks and were routinely reared for two months, then 12 SUI rat model were made. Two months later, transfected with plasmid pEGFP-N1 of HUCMSC were injected into the region surrounding the urinary tract matched with saline injection as control group. To get genitourinary tissue after testing urodynamic indicators, and observe the pathological changes of the bladder, urethra and the surrounding tissue; fluorescent cell of the experimental groups specimens were observed by fluorescence microscope. RESULTS: The leak point pressure(LPP) was (23.8 ± 4.2) mm Hg(1 mm Hg = 0.133 kPa) of the SUI rats. Transplanting mesenchymal stem cells of SUI rats, the positive rate of sneeze test was 1/6 in SUI group and 5/6 in control group, which reached statistical significance (P < 0.05); LPP was (30.6 ± 2.8) mm Hg in SUI group and (21.4 ± 7.0) mm Hg in control group, which reached statistical significance (P < 0.05) .In SUI rate model, connective tissue content were increased in urethra and the surrounding tissue and more fluorescent cell were observed. CONCLUSIONS: A rat model of female SUI was established successfully through postpartum vaginal balloon dilation and bilateral ovariectomy. MSC can be survived and proliferated in the urethral and the surrounding tissue of injured rats, and improve the urodynamic indicators and the positive rate of sneeze test. Morphology shows renovation of the support structures around the urethra.


Assuntos
Transplante de Células-Tronco de Sangue do Cordão Umbilical/métodos , Transplante de Células-Tronco Mesenquimais/métodos , Incontinência Urinária por Estresse/terapia , Animais , Proliferação de Células , Células Cultivadas , Modelos Animais de Doenças , Feminino , Humanos , Ovariectomia , Ratos , Ratos Sprague-Dawley , Transplante Heterólogo , Cordão Umbilical/citologia , Uretra/patologia , Uretra/fisiopatologia , Incontinência Urinária por Estresse/etiologia , Incontinência Urinária por Estresse/fisiopatologia , Urodinâmica , Vagina/lesões , Vagina/fisiopatologia
20.
J Huazhong Univ Sci Technolog Med Sci ; 33(5): 735-742, 2013 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-24142729

RESUMO

Human papillomavirus (HPV)-induced cervical cancer is the second most common cancer among women worldwide. Despite the encouraging development of the preventive vaccine for HPV, a vaccine for both prevention and therapy or pre-cancerous lesions remains in high priority. Thus far, most of the HPV therapeutic vaccines are focused on HPV E6 and E7 oncogene. However these vaccines could not completely eradicate the lesions. Recently, HPV E5, which is considered as an oncogene, is getting more and more attention. In this study, we predicted the epitopes of HPV16 E5 by bioinformatics as candidate peptide, then, evaluated the efficacy and chose an effective one to do the further test. To evaluate the effect of vaccine, rTC-1 (TC-1 cells infected by rAAV-HPV16E5) served as cell tumor model and rTC-1 loading mice as an ectopic tumor model. We prepared vaccine by muscle injection. The vaccine effects were determined by evaluating the function of tumor-specific T cells by cell proliferation assay and ELISPOT, calculating the tumor volume in mice and estimating the survival time of mice. Our in vitro and in vivo studies revealed that injection of E5 peptide+CpG resulted in strong cell-mediated immunity (CMI) and protected mice from tumor growth, meanwhile, prolonged the survival time after tumor cell loading. This study provides new insights into HPV16 E5 as a possible target on the therapeutic strategies about cervical cancer.


Assuntos
Vacinas Anticâncer/imunologia , Papillomavirus Humano 16/imunologia , Proteínas Oncogênicas Virais/imunologia , Infecções por Papillomavirus/imunologia , Vacinas contra Papillomavirus/imunologia , Neoplasias do Colo do Útero/imunologia , Adulto , Idoso , Sequência de Aminoácidos , Animais , Vacinas Anticâncer/administração & dosagem , Linhagem Celular , Linhagem Celular Tumoral , Dependovirus/genética , Feminino , Regulação Viral da Expressão Gênica/imunologia , Vetores Genéticos/genética , Papillomavirus Humano 16/genética , Humanos , Camundongos , Camundongos Endogâmicos C57BL , Pessoa de Meia-Idade , Neoplasias Experimentais/imunologia , Neoplasias Experimentais/prevenção & controle , Neoplasias Experimentais/virologia , Proteínas Oncogênicas Virais/genética , Infecções por Papillomavirus/prevenção & controle , Infecções por Papillomavirus/virologia , Vacinas contra Papillomavirus/administração & dosagem , Análise de Sobrevida , Linfócitos T/imunologia , Linfócitos T/metabolismo , Carga Tumoral/imunologia , Neoplasias do Colo do Útero/prevenção & controle , Neoplasias do Colo do Útero/virologia , Vacinas de Subunidades/administração & dosagem , Vacinas de Subunidades/imunologia
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