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1.
EBioMedicine ; 52: 102651, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32062354

RESUMO

BACKGROUND: Glioma has a poor prognosis, and is the most common primary and lethal primary malignant tumor in the central nervous system. Retinoic acid receptor-related orphan receptor A (RORA) is a member of the ROR subfamily of orphan receptors and plays an anti-tumor role in several cancers. METHODS: A cell viability assay, the Edu assay, neurosphere formation assay, and xenograft experiments were used to detect the proliferative abilities of glioma cell line, glioma stem cells (GSCs). Western blotting, ELISAs, and luciferase reporter assays were used to detect the presence of possible microRNAs. FINDINGS: Our study found for the first time that RORA was expressed at low levels in gliomas, and was associated with a good prognosis. RORA overexpression inhibited the proliferation and tumorigenesis of glioma cell lines and GSCs via inhibiting the TNF-α mediated NF-κB signaling pathway. In addition, microRNA-18a had a promoting effect on gliomas, and was the possible reason for low RORA expression in gliomas. INTERPRETATION: RORA may be a promising therapeutic target in the treatment of gliomas.

2.
Int J Biol Macromol ; 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-32070735

RESUMO

The development of natural and biodegradable polymer nanoparticles as Pickering emulsion stabilizers has attracted increasing interest. In this study, antioxidative pectin from hawthorn wine pomace (HP) was first produced. HP and zein-nanoparticles (ZPs) were used to fabricate zein-HP composite nanoparticles (ZHPs) via hydrogen bonding and electrostatic interaction. The ZHP composite at the HP-ZP ratio of 1:1 (w/w) exhibited near-neutral wettability (92.9o ±â€¯1.01), thereby being used for stabilizing Pickering emulsion (ZHPEs). CLSM and cryo-SEM showed the anchoring of ZHPs onto the surface of oil droplets and the gel-like network structure in the continuous-phase. ZHPEs at 0.5-0.7 (v/v) oil fractions were pseudoplastic fluids with elastic-solid characteristics. ZHPEs with 0.6 and 0.7 (v/v) oil fractions showed excellent thermal stability 20-60 °C. The antioxidant capacity of HP helped protect the Pickering emulsion against its lipid oxidation. Therefore, antioxidative polysaccharides could stabilize Pickering emulsions as particle shell-materials while offering protection on lipid components against oxidation. This study has demonstrated the sustainable utilization of food waste for producing value-added products.

3.
Artigo em Inglês | MEDLINE | ID: mdl-32088759

RESUMO

Single-molecule real-time (SMRT) sequencing can be used to identify a wide variety of chemical modifications of the genome, such as methylation. Here, we applied this approach to identify N6-methyl-adenine (m6A) and N4-methyl-cytosine (m4C) modification in the genome of Bacillus pumilus BA06. A typical methylation recognition motif of the type I restriction-modification system (R-M), 5'-TCm6AN8TTGG-3'/3'-AGTN8m6AACC-5', was identified. We confirmed that this motif was a new type I methylation site using REBASE analysis and that it was recognized by a type I R-M system, Bpu6ORFCP, according to methylation sensitivity assays in vivo and vitro. Furthermore, we found that deletion of the R-M system Bpu6ORFCP induced transcriptional changes in many genes and led to increased gene expression in pathways related to ABC transporters, sulfur metabolism, ribosomes, cysteine and methionine metabolism and starch and sucrose metabolism, suggesting that the R-M system in B. pumilus BA06 has other significant biological functions beyond protecting the B. pumilus BA06 genome from foreign DNA.

4.
Carbohydr Polym ; 233: 115859, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-32059910

RESUMO

Naked-eye detection pH sensor is becoming a powerful tool in food safety monitoring. In this work, a pH sensor was developed by incorporating cellulose modified with acidochromic dye into polyvinyl alcohol (PVA). The results indicated that the dye (up to 170.4 µmol/g) was successfully anchored to cellulose. It was demonstrated that the addition of acidochromic regenerated cellulose (ARC) resulted in enhancement of tensile strength, elongation at break and maximum decomposition temperature by 44 %, 43.6 % and 11 °C, respectively. The pH sensor demonstrated that a visible color change from yellow to brick-red and to purple when placed in solutions of pH = 7, 10 and 12. The pH sensor showed excellent resistance to leaching under strong acidic and alkaline conditions. When applied to spoiled shrimp, an evident color change from yellow to brown was observed, suggesting it could serve as an easy-to-use, non-destructive visual indicator system for real-time food monitoring.

5.
Biotechnol Bioeng ; 2020 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-32048725

RESUMO

In the initial step of sugar metabolism, sugar-specific transporters play a decisive role in the passage of sugars through plasma membranes into cytoplasm. The SecY complex (SecYEG) in bacteria forms a membrane channel responsible for protein translocation. The present work shows that permeabilized SecY channels can be used as nonspecific sugar transporters in Escherichia coli. SecY with the plug domain deleted allowed the passage of glucose, fructose, mannose, xylose, and arabinose, and, with additional pore-ring mutations, facilitated lactose transport, indicating that sugar passage via permeabilized SecY was independent of sugar stereospecificity. The engineered E. coli showed rapid growth on a wide spectrum of monosaccharides and benefited from the elimination of transport saturation, improvement in sugar tolerance, reduction in competitive inhibition, and prevention of carbon catabolite repression, which are usually encountered with native sugar uptake systems. The SecY channel is widespread in prokaryotes, so other bacteria may also be engineered to utilize this system for sugar uptake. The SecY channel thus provides a unique sugar passageway for future development of robust cell factories for biotechnological applications.

6.
Aging (Albany NY) ; 12(1): 156-177, 2020 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-31896739

RESUMO

A promising new strategy for cancer therapy is to target the autophagic pathway. However, comprehensive characterization of autophagy genes and their clinical relevance in cancer is still lacking. Here, we systematically characterized alterations of autophagy genes in multiple cancer lines by analyzing data from The Cancer Genome Atlas and CellMiner database. Interactions between autophagy genes and clinically actionable genes (CAGs) were identified by analyzing co-expression, protein-protein interactions (PPIs) and transcription factor (TF) data. A key subnetwork was identified that included 18 autophagy genes and 22 CAGs linked by 28 PPI pairs and 1 TF-target pair, which was EGFR targeted by RARA. Alterations in the expression of autophagy genes were associated with patient survival in multiple cancer types. RARA and EGFR were associated with worse survival in colorectal cancer patients. The regulatory role of EGFR in 5-FU resistance was validated in colon cancer cells in vivo and in vitro. EGFR contributed to 5-FU resistance in colon cancer cells through autophagy induction, and EGFR overexpression in 5-FU resistant colon cancer was regulated by RARA. The present study provides a comprehensive analysis of autophagy in different cancer cell lines and highlights the potential clinical utility of targeting autophagy genes.

7.
Chem Commun (Camb) ; 56(14): 2087-2090, 2020 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-31967623

RESUMO

A dual-emissive tris-heteroleptic ruthenium complex is designed, synthesized and applied for the ratiometric photoluminescent detection of amyloid-ß (Aß) aggregation in both steady and transient states. The Aß aggregation is supported by transmission electron microscopy and confocal laser scanning microscopy analysis. In addition, molecular docking calculations have been performed to gain insights into the interaction mode between the ruthenium complex and Aß fibrils.

8.
Int J Biol Macromol ; 148: 811-816, 2020 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-31962069

RESUMO

Cellulosic colloidal suspensions present unique opportunities for rheological modification of complex fluids. In this work, the rheological behavior of regenerated cellulose (RC) suspensions, including their oscillating shear and time-dependent behavior, as well as yield stress, were studied. The rheological effects of sodium alginate's addition to aqueous RC solutions subject to shear flow were investigated. The results reveal that the RC suspension exhibited "gel-like" behavior and had a shear-thinning property. At increasing RC concentrations, the suspensions' yield stress and the extent of viscosity recovery after plastic deformation had both increased. The viscoelastic suspensions underwent a transition from "solid-like" to "liquid-like" behavior upon sodium alginate's inclusion. Sodium alginate was found to enhance RC suspensions' viscosity recoverability. Furthermore, with increasing concentrations of sodium alginate, the yield stress of RC suspension began to decrease and then vanished, occurring below the 1:1 RC: sodium alginate weight ratio with total solid content fixed at 1 wt%, due to RC's inability to form an extended network RC. This study yields insights into the rheology of RC suspensions and the influence of sodium alginate and supports both their usage as rheological modifies in applications such as coatings, drug delivery systems, and additive manufacturing techniques such as 3D printing.

9.
J Food Biochem ; 44(1): e13086, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31646664

RESUMO

Rutin is a bioflavonoid found in many plants and derived foods, accordingly, rutin likely interacts with α-amino acids such as Lys, Ile, His or Glu to give Maillard reaction products (MRPs). The heated rutin-Lys system exhibited highest brown intensity and in vitro antioxidant activities. The 30-50 kDa rutin-Lys fraction had higher in vitro antioxidant activities than the other fractions, and at a dose of 0.4 mg/ml preserved over 90% cell viability for HepG2 cells exposed to H2 O2 . The dose-dependent protective effects against H2 O2 -induced oxidative stress of the rutin-Lys MRPs may involve the inhibition of reactive oxygen species generation, enhancement of the superoxide dismutase and catalase activities, along with the activation of the Nrf2-dependent pathway and upregulation of phase II antioxidant genes (including NQO1, HO-1, GCLG, and GCLM). PRACTICAL APPLICATIONS: Rutin is widely distributed in vegetables and grains. The Maillard reaction is a common reaction occurring during food processing, and produces Maillard reaction products (MRPs) with distinct processing and biological properties. This study shows that a 30-min thermal treatment at 120°C generates antioxidative MRPs in the rutin-Lys, rutin-His, rutin-Ile and rutin-Glu model systems, which can directly inhibit reactive oxygen species generation and enhance SOD and CAT activities while activating the Nrf2-dependent pathway and upregulating the expression of phase II detoxifying antioxidant genes. Therefore, for food systems containing phenolic antioxidants and proteins (such as rutin and Lys), one may enhance the antioxidant properties of these food systems through a 30-min thermal treatment at 120°C. Also, the resultant rutin-Lys MRPs may be isolated and used as commercial preparations of natural antioxidants.

10.
Neuro Oncol ; 22(1): 46-57, 2020 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-31400279

RESUMO

BACKGROUND: Deregulation of the nuclear factor of activated T cell (NFAT) pathway has been reported in several human cancers. Particularly, NFAT2 is involved in the malignant transformation of tumor cells and is identified as an oncogene. However, the role of NFAT2 in glioblastoma (GBM) is largely unknown. METHODS: The expression and prognostic value of NFAT2 were examined in the databases of the Repository of Molecular Brain Neoplasia Data and The Cancer Genome Atlas (TCGA) and clinical samples. The functional effects of silencing or overexpression of NFAT2 were evaluated in glioma stem cell (GSC) viability, invasion, and self-renewal in vitro and in tumorigenicity in vivo. The downstream target of NFAT2 was investigated. RESULTS: High NFAT2 expression was significantly associated with mesenchymal (MES) subtype and recurrent GBM and predicted poor survival. NFAT2 silencing inhibited the invasion and clonogenicity of MES GSC-enriched spheres in vitro and in vivo. NFAT2 overexpression promoted tumor growth and MES differentiation of GSCs. A TCGA database search showed that histone deacetylase 1 (HDAC1) expression was significantly correlated with that of NFAT2. NFAT2 regulates the transcriptional activity of HDAC1. Rescue of HDAC1 in NFAT2-knockdown GSCs partially restored tumor growth and MES phenotype. Loss of NFAT2 and HDAC1 expression resulted in hyperacetylation of nuclear factor-kappaB (NF-κB), which inhibits NF-κB-dependent transcriptional activity. CONCLUSION: Our findings suggest that the NFAT2-HDAC1 pathway might play an important role in the maintenance of the malignant phenotype and promote MES transition in GSCs, which provide potential molecular targets for the treatment of GBMs.

11.
Ecotoxicol Environ Saf ; 187: 109820, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31670242

RESUMO

Huge amounts of fly ash (FA) can be annually produced in power plants. Fly ash always contains high levels of arsenic (As) and selenium (Se) due to the preconcentration of these two elements during coal combustion process. It would be much concerned to screen their fractions and potential environmental behaviors in fly ash for beneficial use and treatment. Fast and practical methods for this purpose are urgently needed. Two fast and effective microwave-assisted sequential extraction schemes (MASE) were developed for fast screening As and Se fractions in fly ash for the first time. The extraction parameters including microwave irradiation time, temperature and power energy were optimized by comparing the results from MASE and the conventional scheme (Wenzel method). The results indicate that the extraction efficiency of As and Se in various fractions can be significantly accelerated by microwave irradiation. The whole procedure operation time can be significantly reduced from 24.5 h to 44 min by microwave assistance compared with the conventional shaking schemes. The recoveries of As and Se in the various extracted fractions were all above 80% with relative standard deviations (RSDs) below 8%. The developed methods were further confirmed by the validation of the certified reference material GBW08401 and fly ash samples from six power plants. The developed MASE methods are practical and effective for fast screening arsenic and selenium fractions in fly ash samples.


Assuntos
Arsênico/análise , Cinza de Carvão/análise , Monitoramento Ambiental/métodos , Poluentes Ambientais/análise , Micro-Ondas , Selênio/análise , Disponibilidade Biológica , Centrais Elétricas
12.
J Sci Food Agric ; 100(2): 595-606, 2020 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-31591721

RESUMO

BACKGROUND: Climate change has posed great challenges to rice production. Temperature and solar radiation show significant variations in central China. This study aims to analyze the responses of different rice genotypes to the variations of temperature and solar radiation in central China, and to find the way of identifying the optimal sowing date to improve and stabilize rice production. For this end, four rice genotypes (two Indica and two Japonica cultivars) were cultivated at two locations under irrigation conditions in 2 years with six sowing dates. RESULTS: We investigated variations of rice grain yield, resource use efficiency, average daily temperature and solar radiation during different phenological stages. Rice grain yield could increase by about 2-17% in central China. Compared with solar radiation, temperature was a more important factor affecting rice grain yield in central China. The grain yield showed great correlation with the means temperature during different phenological stages, especially during the first 20 days after heading (GT20). Besides our results demonstrated that the grain yield displayed slender variations when the GT20 was within 24.9-26.4 °C. However, GT20 was higher than 26.4 °C in most cases, which became more frequent due to climate changes. Analysis of climate change during the last 25 years revealed that the frequency of GT20 within 24.9-26.4 °C was increased by the delay of sowing date. CONCLUSION: We propose that delaying sowing date to achieve the optimal GT20 (24.9 °C-26.4 °C) can be an effective strategy to stabilize and improve rice grain yield and resource use efficiency in central China. © 2019 Society of Chemical Industry.


Assuntos
Produção Agrícola/métodos , Oryza/crescimento & desenvolvimento , Irrigação Agrícola , China , Mudança Climática , Genótipo , Oryza/genética , Oryza/metabolismo , Sementes/crescimento & desenvolvimento , Sementes/metabolismo , Temperatura Ambiente , Água/análise , Água/metabolismo
13.
J Dairy Sci ; 103(1): 87-97, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31677844

RESUMO

Previous research reported that fermented yak milk had a diverse microbial composition. For this study, raw yak milk, qula, and fermented yak milk samples were collected from the Aba Tibetan autonomous region of China. The genus and species microbial composition of these samples were analyzed by high-throughput sequencing of 16S rRNA and groEL gene amplicons, and the volatile profile of the samples was quantified by gas chromatography-mass spectrometry. The results indicated variation in abundance of microbiota at the genus level among the fermented yak milk samples, with Lactobacillus as the most abundant genus in the majority of samples, ranging from 41.6 to 98.3%. The volatile profile of the samples varied among those collected from different villages. Correlations between bacterial composition and volatile compounds of the samples were also observed. Lactobacillus displayed a significant correlation with volatile compounds such as benzaldehyde, 2,3-pentanedione, ethanol, and ethyl acetate, whereas the samples with relatively high abundance of Streptococcus and Lactococcus displayed relatively low contents of volatile compounds.

14.
Huan Jing Ke Xue ; 40(6): 2813-2820, 2019 Jun 08.
Artigo em Chinês | MEDLINE | ID: mdl-31854675

RESUMO

Physical properties and bioactivity are two important aspects of the activated sludge process. To solve the contradiction between these two aspects in the operation control process, the physical structure and respiration processes of sludge in different activated sludge systems were analyzed. Sludge from seven different activated sludge systems was used for the experiments, and the particle size, microscopic morphology, fractal dimension, compression settling performance, adsorption performance, and respirogram were studied and analyzed. The results showed that the correlations between physical performance indicators and respiration rate were very good. Sludge particle size (d) was negatively correlated with the endogenous specific respiration rate (SOURe) and maximum specific respiration rate (SOURt) (R2>0.9); fractal dimension (Df) was linearly and negatively correlated with SOURe and SOURt (R2>0.8); sludge compression index (SCI) and sludge volume index (SVI) were positively correlated with SOURe (R2>0.9); equilibrium adsorption (Qmax) was linearly and negatively correlated with the quasi-endogenous specific respiration rate (SOURq) and SOURe (R2>0.9). In addition, the optimal physical properties and bioactivity range of activated sludge under normal operating conditions were obtained as follows:SVI of 50-120 mL·g-1, SOURe of 6.27-7.55 mg·(g·h)-1, d of 205.80-228.12 µm, Df of 1.56-1.60, Rn/t of 0.02-0.03, and Qmax of 508-636 mg·g-1.

15.
Front Pharmacol ; 10: 1260, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31708783

RESUMO

Background: Immune checkpoint inhibitors (ICIs) have evolved for the treatment of solid tumors. In addition to the efficacy of ICIs for cancer, the adverse events (AEs) of ICIs are also noteworthy for gradually more extensive clinical use. Objective: To conduct a systematic review and network meta-analysis to evaluate the treatment-related AEs that occurred in clinical trials using different kinds of ICIs, to explore the differences in AEs among ICIs for treating non-small cell lung cancer (NSCLC) and melanoma, and to compare select immune-related AEs. Methods: PubMed, EMBASE, Cochrane Library, ClinicalTrials.gov, and other available sources were systematically searched for published reports up to January 1, 2019. Two reviewers independently selected reports about phase II/III randomized controlled trials to compare among ICIs and between ICIs and chemotherapy. After the bias assessment of all included trials, a Bayesian network meta-analysis was performed. The primary outcomes were any-grade and high-grade treatment-related AEs from all ICIs. The secondary outcomes were AEs in patients with NSCLC and melanoma and the presence of the select AEs pneumonitis/pneumonia and colitis. Results: Eighteen randomized controlled trials containing 11,223 patients with NSCLC or melanoma were included. A total network meta-analysis was conducted. The meta-analysis showed that atezolizumab 1,200 mg and pembrolizumab 2 mg/kg every 3 weeks were generally more tolerable than other ICIs. ICI combined with chemotherapy might suggest a higher risk of treatment-related AEs than monotherapy with a single ICI, except durvalumab and ipilimumab. In the NSCLC subgroup, pembrolizumab was associated with a higher risk of high-grade AEs than nivolumab. In addition, ICIs (nivolumab, atezolizumab, and avelumab) led to a lower risk of any/high-grade treatment-related AEs than traditional chemotherapy and ICI combination chemotherapy. However, ICIs did not present preferable safety and tolerability compared to chemotherapy in treating melanoma. Compared with chemotherapy, nivolumab, durvalumab, two ICIs, and ICI combined chemotherapy led to more pneumonitis/pneumonia. However, when treating NSCLC, different types of ICIs did not differ significantly regarding the incidence of pneumonitis/pneumonia. A combination of nivolumab and ipilimumab had the highest risk for colitis, while pembrolizumab and atezolizumab had a lower possibility than the other ICIs. Conclusion: Atezolizumab 1,200 mg and pembrolizumab 2 mg/kg every 3 weeks were ordinarily safer than other ICIs. When treating NSCLC, nivolumab had the lowest risk; when treating melanoma, pembrolizumab had the lowest toxicity.

16.
Int J Biol Macromol ; 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31739045

RESUMO

Recently, the use of emulsion as a delivery system for essential oils has attracted increasing attention. In this research, cinnamon essential oil Pickering emulsion (ZCCPEs) stabilized by zein-pectin composite nanoparticles (ZCPs) was constructed as an effective antimicrobial system. Thereafter, the influence of ZCPs concentration on the stability of ZCCPEs was studied. The results showed that 0.25% ZCPs could reduce the bioorganic matter by four times compared with 1% ZCPs while maintaining good physical stability. The inhibitory effect of ZCCPEs on two food-related microorganisms (i.e. Alternaria alternata and Botrytis cinerea) was evaluated by antimicrobial assay. In addition, two fresh-cut apple slices models were constructed to systematically evaluate the application potential of ZCCPEs in food preservation. Due to the well dispersibility and sustained-release ability, ZCCPEs showed superior antibacterial performance than pure essential oil. The fabricated zein-pectin based Pickering emulsions might provide a promising alternative for the delivery of antimicrobial essential oils in the food industries.

17.
Cancer Manag Res ; 11: 8911-8921, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31632151

RESUMO

Purpose: Metadherin (MTDH), as an oncogene, is associated with metastasis and poor prognosis. This study investigated MTDH expressions and development of gastric cancer (GC) cell phenotypes and the contribution of MTDH to epithelial-mesenchymal transition (EMT). Patients and methods: MTDH expression was assayed in human GC cell lines and tumor tissue from 92 GC patients. Functional experiments were performed to characterize MTDH activity. Expressions of EMT-related proteins (vimentin and E-cadherin), phosphorylated ß-catenin and ß-catenin were assayed by immunohistochemistry, Western blotting, immunofluorescence, and co-immunoprecipitation, respectively. Results: MTDH expressions were higher in GC tissue than that in gastric mucosa from the same patient. MTDH overexpression was correlated with metastasis and enhanced malignant GC phenotypes, i.e., proliferation, migration, invasiveness, and chemoresistance. MTDH overexpression was associated with expressions of vimentin, E-cadherin and cancer stem-cell biomarkers including CD44, CD133, and Oct4. MTDH complexed with ß-catenin and decreased phosphorylated ß-catenin levels to facilitate ß-catenin translocation into the nucleus and expressions of downstream genes. Conclusion: MTDH overexpression in GC cells is associated with EMT and development of cancer stem cell malignant phenotypes and affects the subcellular translocation of ß-catenin. The results warrant investigation of the prognostic value of MTDH in GC and as a therapeutic target.

18.
Chem Commun (Camb) ; 55(89): 13406-13409, 2019 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-31637391

RESUMO

A new small molecular hole-transporting material, 1,3,6,8-tetrakis[N-(p-methoxyphenyl)-N'-(9,9'-dimethyl-9H-fluoren-2-yl)-amino]pyrene (TFAP) was synthesized and applied in CH3NH3PbI3-perovskite solar cells. A best power conversion efficiency of 19.7% with a photovoltage of 1.11 V has been achieved.

19.
EBioMedicine ; 48: 36-48, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31631037

RESUMO

BACKGROUND: Glioma is the most common primary malignant tumor in the central nervous system with frequent hypoxia and angiogenesis. Limb-Bud and Heart (LBH) is a highly conserved transcription cofactor that participates in embryonic development and tumorigenesis. METHODS: The conditioned media from LBH regulated human glioma cell lines and patient-derived glioma stem cells (GSCs) were used to treat the human brain microvessel endothelial cells (hBMECs). The function of LBH on angiogenesis were examined through methods of MTS assay, Edu assay, TUNEL assay, western blotting analysis, qPCR analysis, luciferase reporter assay and xenograft experiment. FINDINGS: Our study found for the first time that LBH was overexpressed in gliomas and was associated with a poor prognosis. LBH overexpression participated in the angiogenesis of gliomas via the vascular endothelial growth factor A (VEGFA)-mediated extracellular signal-regulated kinase (ERK) signalling pathway in human brain microvessel endothelial cells (hBMECs). Rapid proliferation of gliomas can lead to tissue hypoxia and hypoxia inducible factor-1 (HIF-1) activation, while HIF-1 can directly transcriptionally regulate the expression of LBH and result in a self-reinforcing cycle. INTERPRETATION: LBH may be a possible treatment target to break the vicious cycle in glioma treatment.  :  .

20.
Phys Rev Lett ; 123(10): 100503, 2019 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-31573287

RESUMO

Quantum computing has seen tremendous progress in past years. Due to implementation complexity and cost, the future path of quantum computation is strongly believed to delegate computational tasks to powerful quantum servers on the cloud. Universal blind quantum computing (UBQC) provides the protocol for the secure delegation of arbitrary quantum computations, and it has received significant attention. However, a great challenge in UBQC is how to transmit a quantum state over a long distance securely and reliably. Here, we solve this challenge by proposing a resource-efficient remote blind qubit preparation (RBQP) protocol, with weak coherent pulses for the client to produce, using a compact and low-cost laser. We experimentally verify a key step of RBQP-quantum nondemolition measurement-in the field test over 100 km of fiber. Our experiment uses a quantum teleportation setup in the telecom wavelength and generates 1000 secure qubits with an average fidelity of (86.9±1.5)%, which exceeds the quantum no-cloning fidelity of equatorial qubit states. The results prove the feasibility of UBQC over long distances, and thus serves as a key milestone towards secure cloud quantum computing.

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