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1.
ACS Nano ; 2022 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-34985244

RESUMO

The development of flexible electronic skins with high performance and multifunctional sensing capabilities is of great significance for applications ranging from healthcare monitoring to artificial intelligence. To mimic and surpass the high-gauge-factor sensing properties of human skin, structure design and appropriate material selection of sensors are both essentially required. Here, we present an efficient, low-cost fabrication strategy to construct an ultra-highly sensitive, flexible pressure sensor by embedding the aligned nickel-coated carbon fibers (NICFs) in a polydimethylsiloxane (PDMS) substrate. Our design substantially contributes to ultrahigh sensitivity through the parallel circuit formed by aligned NICFs as well as surface spinosum microstructure molded by sandpaper. As a result, the sensor exhibits excellent sensitivity (15 525 kPa-1), a fast response time (30 ms), and good stability over 3000 loading-unloading cycles. Furthermore, these superior sensing properties trigger applications in water quality and wave monitoring in conjunction with mechanical flexibility and robustness. As a precedent for adjusting the sensitivities of the sensor, the NICFs/PDMS sensor provides a promising method for multiscenario healthcare monitoring, multiscale pressure spatial distribution, and human-machine interfacing.

3.
Bioengineered ; 13(1): 455-468, 2022 01.
Artigo em Inglês | MEDLINE | ID: mdl-34967265

RESUMO

Numerous studies have reported the regulatory effects of miR-21-5p and reversine in human breast cancer (HBC). However, the mechanism of reversine and miR-21-5p has not been fully investigated in HBC. The aim of the current study was to assess the mechanism of action of reversine, with or without miR-21-5p, in HBC progression. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and Western blot results confirmed the upregulation of miR-21-5p and downregulation of sprouty RTK signaling antagonist 2 (SPRY2) in HBC. Bioinformatics analysis and luciferase assay identified the correlation between miR-21-5p and SPRY2. Cell function experiment results indicated a decrease in migration, proliferation, and invasion of HBC cells treated with miR-21-5p inhibitor and reversine; however, an increase in apoptosis was observed in these cells. Apoptotic ability was more enhanced and migration, proliferation, and invasion were more impaired in HBC cells treated with both miR-21-5p inhibitor and reversine than in those treated individually with either inhibitors. SPRY2, downstream of miR-21-5p, participated in HBC progression with reversine. Overall, our study proved that combining the miR-21-5p inhibitor with reversine produced a synergistic effect by regulating SPRY2, thereby limiting HBC progression. This knowledge might offer insights into the clinical therapy of HBC.

4.
Sci Rep ; 11(1): 23248, 2021 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-34853382

RESUMO

Nanopesticide is one of the best pesticide formulation technologies to overcome the disadvantages of traditional pesticides, which has received great attention from the international community. Using high-speed emulsification and ultrasonic dispersion technology, an avermectin nano-delivery system (Av-NDs) with a particle size of 80-150 nm was prepared through embedding the pesticide molecule utilizing the cross-linking reaction between sodium lignosulfonate and p-phenylenediamine diazonium salt. The formulation and composition of Av-NDs were optimized, the morphology of Av-NDs was analyzed by scanning electron microscope, transmission electron microscope and dynamic light scattering, and the structure of Av-NDs was characterized by UV, IR and 1H NMR. Anti-photolysis and controlled-release tests show that the stability of Av-NDs is 3-4 times of the original avermectin (Av) and possesses the pH-responsive controlled release property. Furthermore, the insecticidal activity of Av-NDs is better than that of avermectin suspension concentrate (Av-SC). The Av-NDs with anti-photolysis and controlled-release characteristics is suitable for large-scale industrial production and is capable to be utilized as effective insecticide in the field.

5.
Nanomicro Lett ; 14(1): 23, 2021 Dec 09.
Artigo em Inglês | MEDLINE | ID: mdl-34888757

RESUMO

In conventional ethylene carbonate (EC)/propylene carbonate (PC) electrolyte, sodium metal reacts spontaneously and deleteriously with solvent molecules. This significantly limits the practical feasibility of high-voltage sodium metal batteries based on Na metal chemistry. Herein, we present a sodium metal alloy strategy via introducing NaIn and Na2In phases in a Na/In/C composite, aiming at boosting Na ion deposition stability in the common EC/PC electrolyte. Symmetric cells with Na/In/C electrodes achieve an impressive long-term cycling capability at 1 mA cm-2 (> 870 h) and 5 mA cm-2 (> 560 h), respectively, with a capacity of 1 mAh cm-2. In situ optical microscopy clearly unravels a stable Na ion dynamic deposition process on the Na/In/C composite electrode surface, attributing to a dendrite-free and smooth morphology. Furthermore, theoretical simulations reveal intrinsic mechanism for the reversible Na ion deposition behavior with the composite Na/In/C electrode. Upon pairing with a high-voltage NaVPOF cathode, Na/In/C anode illustrates a better suitability in SMBs. This work promises an alternative alloying strategy for enhancing Na metal interfacial stability in the common EC/PC electrolyte for their future applications.

6.
Food Res Int ; 150(Pt A): 110764, 2021 12.
Artigo em Inglês | MEDLINE | ID: mdl-34865781

RESUMO

A field experiment was conducted to explore the impact on rice quality using high-quality (HH) or drought-resistant (HY) cultivars under flooding irrigation (F) or dry cultivation (D) in ratooning rice system by evaluating the metabolism or physicochemical traits of starch, fatty acids, and amino acids affecting grain quality. Compared to FHY and DHY in the main or ratoon season, DHH in ratoon season (DHHR) exhibited a higher appearance and processing quality but lower cooking quality. DHHR mainly synthesized long branch chain amylopectin to construct the crystalline regions with increased crystallinity, crystallites size, interplanar spacing, dislocation density, Asp and Thr in brown and head rice. Also, it accumulated more of C16:0, C18:0, C18:1, C18:2, and C18:3 but reduced glutelin in head rice. An increase in functional groups and diversity was seen in brown and head rice, respectively. Overall, these traits improved the processing, appearance, and pasting quality of DHHR.

7.
Bioengineered ; 12(2): 11599-11611, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34866538

RESUMO

Hepatocellular carcinoma (HCC) is one of the most common malignant tumors worldwide, and chemoprevention represents a feasible treatment to reduce the mortality of this carcinoma. Mulberry fruit polysaccharides (MFP) possess immunoregulatory and anti-inflammatory effects, which have been reported to alleviate liver damage evoked by CCl4 or alcohol in previous reports. However, its chemopreventive effect against liver carcinogenesis is insufficient. The present study was aimed to investigate the possible role of MFP as a pro-apoptosis, and anti-inflammatory agent to possess its chemoprevention property. Hepatocarcinogenesis was induced by diethylnitrosamine/phenobarbital (DEN/PB) for 14 weeks. The DEN/PB-administered rats were co-treated with different doses of MFP (50 or 100 mg/kg body weight) by oral gavage for 14 weeks. Basic hepatic function indexes (AST, ALT, ALP, GGT, total bilirubin, and albumin), and hepatic tumor biomarkers (AFP, CEA, and CA19.9), together with histological assessment were performed. Besides, the hepatic apoptosis markers (Bcl-2, Bax, caspase3, and caspase9), inflammation markers (IL-1ß, TNF-α, and NF-κB), and mutT homologue gene 1 (MTH1) were examined. Oral gavage of MFP inhibited the elevations of hepatic function indexes and hepatic tumor biomarkers and alleviated pathological changes in hepatic tissue. In addition, the hepatic apoptosis markers, inflammation markers, and the mRNA level of MTH1 were abnormal in DEN/PB group, which were reversed by MFP treatment. In conclusion, MFP is an effective agent that provides chemoprevention against DEN/PB-evoked hepatocarcinogenesis via inhibition of inflammation and induction of apoptosis.

8.
Bioengineered ; 12(2): 10420-10429, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34872444

RESUMO

Atherosclerosis (AS) is a typical vascular disease. Emerging evidence has shown that circRNAs play key roles in the progression of AS, but the potential function and underlying mechanism of hsa_circ_0001879 remains unknown. We detected the expression level of hsa_circ_0001879 was determined by qRT-PCR, and the proliferation rate and migration ability of HUVECs were measured by CCK-8 assay and Transwell assay, respectively. Proliferative markers and epithelium mesenchymal transition (EMT) markers were measured through immunoblotting. A dual luciferase activity assay was performed to detect the interaction between circRNAs, miRNAs, and mRNAs. Hsa_circ_0001879 was upregulated in AS patients. Hsa_circ_0001879 inhibited the proliferation and migration ability of Human umbilical vein endothelial cells (HUVECs). Hsa_circ_0001879 directly bound to miR-6873-5p and acted as a sponge. miR-6873-5p-induced HDAC9 mRNA degradation was inhibited by hsa_circ_0001879. Hsa_circ_0001879 decreased the proliferation and migration of HUVECs by inhibiting miR-6873-5p-induced HDAC9 degradation.

9.
J Cutan Med Surg ; : 12034754211064309, 2021 Dec 08.
Artigo em Inglês | MEDLINE | ID: mdl-34878936
10.
Vaccine ; 2021 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-34857419

RESUMO

INTRODUCTION: Due to the lack of understanding of the protective effects and safety of 23-valent pneumococcal polysaccharide vaccine (PPV23) in immune-deficient populations, the vaccination rate of PPV23 among HIV-infected patients is still very low in China. The main objectives of this study were to determine whether the efforts to assess measures for the prevention of pneumococcal pneumonia are still worthwhile, and provide designated vaccination program of HIV-infected persons for government policy based on. METHODS: 60 HIV-infected adults in Lanshan county who had never been vaccinated with any pneumococcal vaccine were enrolled in this study, voluntary vaccination of PPV23 and One-year follow-up after vaccination can be completed. RESULT: 76.67% patients (46/60) had serologic response at 12 months after vaccine, CD4 count(≤500 cells/ul or > 500 cells/ul) and Month from diagnosis to first antiviral therapy (≤1 month or > 1 month) were related to antibody responses (p < 0.05).In this study, PPV23 was well tolerated, no adversereaction was reported.11 Streptococcus pneumoniae pneumonia (9.17%,11/120) occurred in the Unvaccinated group and 1 case(1.67%,1/60)in the vaccination group within one year after vaccination(Fisher's exact probability, P = 0.225). The VE was 81.79%. The per capita benefit was 39.32 dollars, thebenefit-costratio = 1.19. There are significant statistical differences between the vaccinated group and the non-vaccinated group in outpatient costs (p < 0.05, 95 %CI: 9.29-32.11), Medicine costs (p = 0.017, 95 %CI: 2.47-24.44), and disease related indirect costs (p = 0.038, 95 %CI: 0.93-33.63) within one year of vaccination. CONCLUSION: Our study results showed that PPV23 can be safely and effectively administered to HIV-1 infected individuals and effectively preventing Streptococcal pneumonia. Considering the cost-benefit of vaccination among HIV-infected persons, as it has been reported in our study, it is necessary to promote the widespread use of the vaccine among HIV-infected persons in the future.

11.
J Phys Chem B ; 2021 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-34860517

RESUMO

Ions are of central importance in nature, and a variety of potential models was proposed to model ions in different phases for an in-depth exploration of ion-related systems. Here, we developed point charge models of 14 monovalent ions with the traditional 12-6 Lennard-Jones (LJ) potential for use in conjunction with 11 water models of TIP3P, OPC3, SPC/E, SPC/Eb, TIP3P-FB, a99SB-disp, TIP4P-Ew, OPC, TIP4P/2005, TIP4P-D, and TIP4P-FB. The designed models reproduced the real hydration free energy (HFE) of ions and the ion-oxygen distance (IOD) in the first hydration shell accurately and simultaneously, a performance similar to the previously reported 12-6-4 LJ-type ion models (12-6 LJ plus an attractive C4 term for cations or a repulsive one for anions). This work, along with our previous work on di-, tri-, and tetravalent metal cations (J. Chem. Inf. Model. 2021, 61, 4031-4044; J. Chem. Inf. Model. 2021, 61, 4613-4629), demonstrates the feasibility of the simple 12-6 LJ potential in ion modeling. In order for the 12-6 LJ potential to reproduce both the HFE and IOD, the LJ R parameters need to be close to Shannon's ionic radii for the highly charged cations and to the Stokes's van der Waals (vdW) radii for the monovalent ions. With an additional C4 term, the R parameters of 12-6-4 LJ ion models agree well with the Stokes's vdW radii and have a more physical meaning. It appears that the C4 term can be merged into the 12-6 LJ potential by a rational tuning of R and the LJ well depth. Simulations of the osmotic coefficients of alkali chloride solutions and the properties of gaseous and solid alkali halides indicate the necessity of further optimizing ion-ion interactions via, for instance, targeting more properties or using a more physical (polarizable) model.

12.
J Exp Clin Cancer Res ; 40(1): 390, 2021 Dec 10.
Artigo em Inglês | MEDLINE | ID: mdl-34893086

RESUMO

BACKGROUND: Centromere protein N (CENP-N) has been reported to be highly expressed in malignancies, but its role and mechanism in nasopharyngeal carcinoma (NPC) are unknown. METHODS: Abnormal CENP-N expression from NPC microarrays of GEO database was analyzed. CENP-N expression level was confirmed in NPC tissues and cell lines. Stable CENP-N knockdown and overexpression NPC cell lines were established, and transcriptome sequencing after CENP-N knockdown was performed. In vitro and in vivo experiments were performed to test the impact of CENP-N knockdown in NPC cells. ChIP and dual luciferase reporter assays were used to verify the combination of IRF2 and CENP-N. Western blot analysis, cellular immunofluorescence, immunoprecipitation and GST pulldown assays were used to verify the combination of CENP-N and AKT. RESULTS: CENP-N was confirmed to be aberrantly highly expressed in NPC tissues and cell lines and to be associated with high 18F-FDG uptake in cancer nests and poor patient prognosis. Transcriptome sequencing after CENP-N knockdown revealed that genes with altered expression were enriched in pathways related to glucose metabolism, cell cycle regulation. CENP-N knockdown inhibited glucose metabolism, cell proliferation, cell cycling and promoted apoptosis. IRF2 is a transcription factor for CENP-N and directly promotes CENP-N expression in NPC cells. CENP-N affects the glucose metabolism, proliferation, cell cycling and apoptosis of NPC cells in vitro and in vivo through the AKT pathway. CENP-N formed a complex with AKT in NPC cells. Both an AKT inhibitor (MK-2206) and a LDHA inhibitor (GSK2837808A) blocked the effect of CENP-N overexpression on NPC cells by promoting aerobic glycolysis, proliferation, cell cycling and apoptosis resistance. CONCLUSIONS: The IRF2/CENP-N/AKT axis promotes malignant biological behaviors in NPC cells by increasing aerobic glycolysis, and the IRF2/CENP-N/AKT signaling axis is expected to be a new target for NPC therapy.

14.
Front Med (Lausanne) ; 8: 778666, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34912833

RESUMO

Purpose: To evaluate the effect of femtosecond laser-assisted in situ keratomileusis (FS-LASIK) on retinal fovea thickness, volume, and retinal nerve fibre layer (RNFL) thickness. Methods: Thirty-seven eyes (37 patients) undergoing FS-LASIK were included in this prospective study. Optical coherence tomography (OCT) was performed 1 day before, 1 h and 1 day after FS-LASIK surgery. Result: Eighteen male and nineteen females were enrolled. Mean patient age was 22.94 ± 4.22 years. One hour postoperatively, macula fovea thicknesses, macula fovea volume, macula parafovea thickness, macula parafovea volume, macula perifovea thickness, macula perifove volume, temporal RNFL thickness, and superior RNFL thickness measures showed significant decrease (t = 6.171, 6.032, and 9.837, 9.700, 2.532, 4.393, 4.926, 2.265; p = 0.000, 0.000, 0.000, 0.000, 0.016, 0.000, 0.000, and 0.011). Day 1 post-operation, macula fovea thicknesses, macula fovea volume, macula parafovea thickness, macula parafovea volume, and inferior RNFL thickness measures showed significant change compared to preoperative measures (t = 3.620, 3.220, 2.901, 2.910, 3.632; p = 0.001, 0.003, 0.006, 0.006, and 0.001). Conclusion: Our data suggest there are alterations in retinal foveal and RNFL measurements by OCT 1 h and 1 day after FS-LASIK surgery.

15.
Foods ; 10(12)2021 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-34945579

RESUMO

Few studies have investigated the peptidomics of fermented milk by Lactobacillus delbrueckii. The aim of the present study was to interpret the peptidomic pattern of the fermented milk by five strains of L. delbrueckii ssp. bulgaricus and ssp. lactis prior to and after the simulated gastrointestinal digestion in vitro. The results indicated variations in the peptidomics among the samples, particularly between the samples of different subspecies. The peptides originating from ß-casein were abundant in the samples of ssp. bulgaricus, whereas the peptides derived from αs1-casein and αs2-casein were more likely to dominate in those of ssp. lactis. For ß-casein, the strains of ssp. bulgaricus displayed extensive hydrolysis in the regions of (73-97), (100-120), and (130-209), whereas ssp. lactis mainly focused on (160-209). The digestion appears to reduce the variations of the peptidomics profile in general. Among the five strains, L. delbrueckii ssp. bulgaricus DQHXNS8L6 was the most efficient in the generation of bioactive peptides prior to and after digestion. This research provided an approach for evaluating the peptide profile of the strains during fermentation and digestion.

16.
Chemosphere ; 290: 133343, 2021 Dec 16.
Artigo em Inglês | MEDLINE | ID: mdl-34922963

RESUMO

It is necessary to reveal the responses of the biomass production and metal accumulation capacity of different plants to the variations of atmospheric conditions and soil metals, with the acceleration of urbanization and industrialization. In the present study, a series of experiments were designed to study the individual and interactive influences of O3 and CO2 fumigation on the biomass yield, variation in different leaf types, distribution of cadmium (Cd) in various tissues, and phytoremediation efficiency of Festuca arundinacea using open top chambers. The results found that an elevated O3 content of 80 ppb, a potential O3 content predicted for 2050, decreased the total dry mass of F. arundinacea and increased the proportion of falling leaf tissues of the species significantly. Under the same ambient CO2 levels, O3 fumigation increased the Cd concentrations in the roots and the fresh, mature, senescent, and dead leaf tissues by 27.8%, 133.3%, 94.4%, 125.3%, and 48.6%, respectively. An elevated CO2 content (550 ppm) promoted the biomass yield of F. arundinacea, particularly in the falling leaf tissues. The results of the combined O3 and CO2 treatment showed that CO2 fumigation alleviated the negative effects of O3 on plant growth and increased the accumulation capacity in different plant tissues. Significantly more Cd was accumulated in senescent and dead leaves under the synergistic action of CO2 and O3, suggesting that the phytoremediation effect on F. arundinacea using the falling leaves harvesting method could be improved under the future atmospheric environment of high CO2 and O3 levels.

17.
Small Methods ; 5(4): e2001050, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-34927856

RESUMO

While sodium metal anodes (SMAs) feature many performance advantages in sodium ion batteries (SIBs), severe safety concerns remain for using bulk sodium electrodes. Herein, a 3D Ag@C natrophilic substrate prepared by a facile thermal evaporation deposition method, which can be employed as a much safer "anode-free" SMA, is reported. Initially, there is no bulk sodium on the Ag@C substrate in the assembled SIBs. Upon charging, sodium will be uniformly deposited onto the Ag@C substrate and afterwards functions as a real SMA, thus inheriting the intrinsic merits of SMA and enhancing safety simultaneously. While cycling, the as-synthesized substrate demonstrates superior sodium plating/stripping cycling stability at 1, 2 and 3 mA cm-2 with a capacity of 2 mAh cm-2 . Theoretical simulations reveal that Na ions prefer to bind with Ag and form a Na-Ag network, thus clearly revealing uniform sodium deposition on the Ag@C substrate. More importantly, a full battery based on Ag@C and Prussian white with impressive Coulomb efficiency (CE), high rate capability (from 0.1 C to 5 C) and long-term cycling life is illustrated for the first time, thus making Ag@C feasible for the establishment of "anode-free" SIBs with reduced cost, high gravimetric/volumetric energy density and enhanced safety.

18.
J Immunol Res ; 2021: 8078646, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34938816

RESUMO

Laryngeal squamous cell carcinoma (LSCC) is the main type of laryngeal cancer with poor prognosis. Incidence of LSCC increases every year, posing a great threat to human health. The underlying mechanism needs further study. Neutrophils are the most prevalent type of immune cells, which play vital roles in crosstalk between the microenvironment and cancer cells. In our study, we aim to figure out the complex regulation between neutrophils and LSCC. Our experiments showed that LSCC cells could promote the activation and mobility of neutrophils. And, in return, neutrophils enhanced the proliferation, migration, and invasion of LSCC. The subsequent results showed that IL-17 was highly expressed in neutrophil conditioned medium. Block of IL-17 could effectively inhibit the progression of LSCC induced by neutrophils. What is more, the results showed that IL-17 activated the JAK/STAT3 pathway in LSCC. Inhibition of the JAK/STAT3 pathway could significantly block neutrophil-induced LSCC progression. Our research reveals the complex interaction between neutrophils and LSCC cells, providing new ideas for the treatment of LSCC.

19.
Angew Chem Int Ed Engl ; : e202112966, 2021 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-34931421

RESUMO

Syndioselective polymerization of unprotected polar styrenes has achieved great success via specially designed catalysts and the establishment of "self-assisted" theory. In contrast, isospecific polymerization of polar styrenes has remained less explored. Herein, we present new racemic ansa-bis(benz[e]indenyl) rare-earth metal complexes and their high activity and perfect isoselectivity (mmmm>99 %) for the polymerization of unmasked polar styrenes without any activators. Moreover, the copolymerizations of para- and meta-methoxystyrenes with styrene give gradient and random copolymers, respectively. The insertion rate of polar monomers could be readily tuned in the range of 0-100 mol % by changing their loading ratios. The resultant isotactic polar polystyrenes are quantitatively transformed into hydroxyl or methylsulfonyl polystyrenes with high Tg s. DFT calculations reveal the isospecific mechanism.

20.
Foods ; 10(11)2021 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-34829163

RESUMO

Rice quality is a complex indicator, and people are paying more and more attention to the quality of rice. Therefore, we used seven rice varieties for twelve nitrogen fertilizer treatments and obtained eighty-four rice types with seventeen qualities. It was found that 17 quality traits had different coefficients of variation. Among them, the coefficient of variation of chalkiness and protein content was the largest, 44.60% and 17.89% respectively. The cluster analysis method was used to define four categories of different rice qualities. The principal component analysis method was used to comprehensively evaluate 17 qualities of 84 rice. It was found that rice quality was better under low nitrogen conditions, Huanghuazhan and Lvyinzhan were easier to obtain better comprehensive rice quality during cultivation. Future rice research should focus on reducing protein content and increasing peak viscosity.

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