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1.
FASEB J ; 35(9): e21332, 2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-34423867

RESUMO

Emerging research has highlighted the capacity of microRNA-23a-3p (miR-23a-3p) to alleviate inflammatory pain. However, the molecular mechanism by which miR-23a-3p attenuates inflammatory pain is yet to be fully understood. Hence, the current study aimed to elucidate the mechanism by which miR-23a-3p influences inflammatory pain. Bioinformatics was initially performed to predict the inflammatory pain related downstream targets of miR-23a-3p in macrophage-derived extracellular vesicles (EVs). An animal inflammatory pain model was established using Complete Freund's Adjuvant (CFA). The miR-23a-3p expression was downregulated in the microglia of CFA-induced mice, after which the inflammatory factors were determined by ELISA. FISH and immunofluorescence were performed to analyze the co-localization of miR-23a-3p and microglia. Interestingly, miR-23a-3p was transported to the microglia via M2 macrophage-EVs, which elevated the mechanical allodynia and the thermal hyperalgesia thresholds in mice model. The miR-23a-3p downstream target, USP5, was found to stabilize HDAC2 via deubiquitination to promote its expression while inhibiting the expression of NRF2. Taken together, the key findings of the current study demonstrate that macrophage-derived EVs containing miR-23a-3p regulates the HDAC2/NRF2 axis by decreasing USP5 expression to alleviate inflammatory pain, which may provide novel therapeutic targets for the treatment of inflammatory pain.


Assuntos
Vesículas Extracelulares/metabolismo , Histona Desacetilase 2/metabolismo , Inflamação/metabolismo , Macrófagos/citologia , Fator 2 Relacionado a NF-E2/metabolismo , Dor/metabolismo , Proteases Específicas de Ubiquitina/metabolismo , Animais , Linhagem Celular , Enzimas Desubiquitinantes/metabolismo , Modelos Animais de Doenças , Regulação para Baixo , Estabilidade Enzimática , Vesículas Extracelulares/genética , Inflamação/genética , Inflamação/terapia , Macrófagos/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Microglia/citologia , Microglia/metabolismo , Modelos Biológicos , Dor/genética , Manejo da Dor , Proteases Específicas de Ubiquitina/genética , Ubiquitinação
2.
Data Brief ; 30: 105377, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32258267

RESUMO

This data article compiles the detailed and descriptive experimental data of Wikipedia-based semantic similarity approach called as Neighbourhood Aggregated Semantic Contribution (NASC), presented in Husain, et al. [1]. The JWPL (Java Wikipedia Library)-DataMachine and JWPL WikipediaAPI are used to extract the required Wikipedia features from Wikipedia dump. The dataset presents the disambiguated Wikipedia concepts of the gold standard word similarity benchmarks MC30 (English), RG65es (Spanish) and RG65fr (French) and their associated set of categories in the corresponding Wikipedia category graph (WCG). The dataset also contains the number of ancestors, common ancestors, pages, and common pages in the k-neighbourhood of the associated categories for different levels of parameter k in the English, Spanish, and French WCGs. The presented dataset can be used to assess the semantic similarity between Wikipedia concepts in English (MC30), Spanish (RG65es), and French (RG65fr) languages benchmarks. Moreover, the dataset will be useful for the further analysis and comparison of the taxonomic structures of the English, Spanish, and French WCGs.

3.
Inf Sci (N Y) ; 179(5): 600-612, 2009 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-32226108

RESUMO

The current research progress and the existing problems of uncertain or imprecise knowledge representation and reasoning in description logics are analyzed in this paper. Approximate concepts are introduced to description logics based on rough set theory, and a kind of new rough description logic RDL AC (rough description logic based on approximate concepts) is proposed based on approximate concepts. The syntax, semantics and properties of the RDL AC are given. It is proved that the approximate concept satisfiability (definitely satisfiability and possibly satisfiability) reasoning problem and approximate concepts rough subsumption reasoning problem w.r.t. rough TBox in RDL AC may be reduced to the concept satisfiability reasoning problem in (almost) standard ALC (the description logic that provides the Boolean concept constructors plus the existential and universal restriction constructors). The works of this paper provide logic foundations for approximate ontologies and theoretical foundations for reasoning algorithms of more expressive rough description logics including approximate concepts, number restrictions, nominals, inverse roles and role hierarchies.

4.
Fuzzy Sets Syst ; 160(23): 3403-3424, 2009 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-32287529

RESUMO

It is generally accepted that the management of imprecision and vagueness will yield more intelligent and realistic knowledge-based applications. Description Logics (DLs) are suitable, well-known logics for managing structured knowledge that have gained considerable attention the last decade. The current research progress and the existing problems of uncertain or imprecise knowledge representation and reasoning in DLs are analyzed in this paper. An integration between the theories of fuzzy DLs and rough DLs has been attempted by providing fuzzy rough DLs based on fuzzy rough set theory. The syntax, semantics and properties of fuzzy rough DLs are given. It is proved that the satisfiability, subsumption, entailment and ABox consistency reasoning in fuzzy rough DLs may be reduced to the ABox consistency reasoning in the corresponding fuzzy DLs.

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