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1.
Mol Cell ; 2020 Jul 28.
Artigo em Inglês | MEDLINE | ID: mdl-32735772

RESUMO

Autophagic degradation of the endoplasmic reticulum (ER-phagy) is triggered by ER stress in diverse organisms. However, molecular mechanisms governing ER stress-induced ER-phagy remain insufficiently understood. Here we report that ER stress-induced ER-phagy in the fission yeast Schizosaccharomyces pombe requires Epr1, a soluble Atg8-interacting ER-phagy receptor. Epr1 localizes to the ER through interacting with integral ER membrane proteins VAPs. Bridging an Atg8-VAP association is the main ER-phagy role of Epr1, as it can be bypassed by an artificial Atg8-VAP tether. VAPs contribute to ER-phagy not only by tethering Atg8 to the ER membrane, but also by maintaining the ER-plasma membrane contact. Epr1 is upregulated during ER stress by the unfolded protein response (UPR) regulator Ire1. Loss of Epr1 reduces survival against ER stress. Conversely, increasing Epr1 expression suppresses the ER-phagy defect and ER stress sensitivity of cells lacking Ire1. Our findings expand and deepen the molecular understanding of ER-phagy.

2.
Arch Microbiol ; 2020 Jul 03.
Artigo em Inglês | MEDLINE | ID: mdl-32617606

RESUMO

A Gram-staining-positive, non-motile, coccus or short-rod-shaped bacterium, designated H1T, was isolated from a humus soil sample in the Detaille Island of Antarctica. The 16S rRNA gene sequence result indicated that strain H1T shared the highest 16S rRNA gene sequence identity with the type strain of Deinococcus alpinitundrae (96.2%). Growth of strain H1T occurred at 4-25 °C, pH 6.0-8.0 and in the presence of 0-1.0% NaCl (w/v). The respiratory quinone was MK-8. The major fatty acids were C16:0, C17:0 cyclo and summed feature 3 (C16:1ω7c/C16:1ω6c). The polar lipids were aminoglycophospholipid, aminophospholipid, glycolipid and glycophospholipid. The cell wall peptidoglycan type was A3ß. The genomic DNA G + C content was 61.3 mol%. The average nucleotide identity (ANI) between strain H1T and the closely related Deinococcus members was below the cut-off level (95-96%) for species identification. Based on the above results, strain H1T represents a novel species of the genus Deinococcus, for which the name Deinococcus detaillensis sp. nov. is proposed. Type strain is H1T (= CGMCC 1.13938T = JCM 33291T).

3.
Tree Physiol ; 2020 Jul 24.
Artigo em Inglês | MEDLINE | ID: mdl-32705132

RESUMO

The present study aimed to explore the possible functions of radial oxygen loss (ROL) on mangrove nutrition. A field survey was conducted to explore the relations among ROL, root anatomy and leaf N in different mangrove species along a continuous tidal gradient. Three mangroves with different ROL (Avicennia marina > Kandelia obovata > Rhizophora stylosa) were then selected to further explore the dynamics of N at root-soil interface. The results showed that seaward pioneer mangrove species such as A. marina appeared to exhibit higher leaf N despite growing under worse nutrient conditions. Greater leaf N in pioneer mangroves coincided with their special root structure (e.g., high porosity together with a thin lignified/suberized exodermis) and powerful ROL. An interesting positive relation was observed between ROL and leaf N in mangroves. Moreover, the data from rhizo-box further showed that soil nitrification was also strongly correlated with ROL. A. marina, which represented the highest ROL among the three mangrove species studied, consistently possessed the highest NO3-, nitrification and ammonia-oxidizing bacteria and archaea (AOB and AOA) gene copies in the rhizosphere. Besides, both NO3- and NH4+ influxes were found to be higher in the roots of A. marina when compared to those of K. obovata and R. stylosa. In summary, greater N acquisition by pioneer mangroves such as A. marina was strongly correlated with ROL which would regulate N transformation and translocation at root-soil interface. The implications of this study may be significant in mangrove nutrition and the mechanisms involved in mangrove zonation.

4.
J Hazard Mater ; 401: 123292, 2020 Jun 23.
Artigo em Inglês | MEDLINE | ID: mdl-32645546

RESUMO

Herein, a high-performance porous biochar described as PBCKOH was successfully synthesized by two-step pyrolysis of corn straw with chemical activation of KOH, and was employed for the elimination of Cr(VI) and naphthalene (NAP) from water. Benefiting from KOH activation, the PBCKOH was found to possess huge specific surface area of 2183.80 m2/g and many well-developed micropores with average particle size of 2.75 nm and main pore diameters distribution from 1 to 2 nm. The PBCKOH presented an excellent adsorption performance with a theoretical monolayer uptake of 116.97 mg/g for Cr(VI) and a heterogeneous adsorption capacity of 450.43 mg/g for NAP. The uptake equilibrium was attained within about 120 min for Cr(VI), while about 180 min for NAP following avrami fractional-order model, revealing the existence of multiple kinetics during the adsorption. The thermodynamic results showed that the uptake of both Cr(VI) and NAP occurred spontaneously (-ΔG°), while in an endothermic nature for Cr(VI) (+ΔH°) and an exothermic characteristic for NAP (-ΔH°) with different randomness. Furthermore, the PBCKOH was believed to enhance the Cr(VI) adsorption mainly through the combination of electrostatic attraction, complexation, ion exchange and reduction action, while achieving the high NAP uptake by pore filling and π-π stacking interactions.

5.
Genome Biol Evol ; 2020 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-32687170

RESUMO

Rhizobia are soil bacteria capable of forming symbiotic nitrogen-fixing nodules associated with leguminous plants. In fast-growing legume-nodulating rhizobia, such as the species in the family Rhizobiaceae, the symbiotic plasmid is the main genetic basis for nitrogen-fixing symbiosis, and is susceptible to horizontal gene transfer. To further understand the symbioses evolution in Rhizobiaceae, we analysed the pan-genome of this family based on 92 genomes of type/reference strains and reconstructed its phylogeny using a phylogenomics approach. Intriguingly, although the genetic expansion that occurred in chromosomal regions was the main reason for the high proportion of low-frequency flexible gene families in the pan-genome, gene gain events associated with accessory plasmids introduced more genes into the genomes of nitrogen-fixing species. For symbiotic plasmids, although horizontal gene transfer frequently occurred, transfer may be impeded by, such as, the host's physical isolation and soil conditions, even among phylogenetically close species. During coevolution with leguminous hosts, the plasmid system, including accessory and symbiotic plasmids, may have evolved over a time span, and provided rhizobial species with the ability to adapt to various environmental conditions and helped them achieve nitrogen fixation. These findings provide new insights into the phylogeny of Rhizobiaceae and advance our understanding of the evolution of symbiotic nitrogen fixation.

6.
J Hazard Mater ; 400: 123142, 2020 Jun 11.
Artigo em Inglês | MEDLINE | ID: mdl-32593944

RESUMO

Heavy metals and pesticides often coexist in contaminated water, while their potential competition behaviors make the adsorptive removal more challenging. Thus, decorating an adsorbent with independent functional sites could be a promising alternative to radically prevent the competitive process for improving the adsorption performance. Herein, ß-cyclodextrin functionalized rice husk-based cellulose (ß-CD@RH-C) was designed and applied for synchronous removal of atrazine and Pb(II). The characterization results supported the successful grafting of ß-cyclodextrin onto the cellulose. The ß-CD@RH-C presented a pH-dependent adsorption performance for Pb(II) with a theoretical monolayer adsorption capacity of 283.00 mg/g, while was mostly unrelated to pH for atrazine adsorption with a heterogeneous uptake of 162.21 mg/g in the mono-component system. Most importantly, the ß-CD@RH-C could efficiently achieve simultaneous removal of atrazine and Pb(II) via avoiding their competitive behaviors, which was due to the different adsorption mechanisms for atrazine (i.e. host-guest interaction) and Pb(II) (i.e. complexation and electrostatic interaction). Moreover, the adsorbed atrazine and Pb(II) could be sequentially desorbed with slight decrease in the adsorption performance of ß-CD@RH-C even after four cycles in the atrazine-Pb(II) multi-component system. All these results suggested ß-CD@RH-C to be a tailored adsorbent with high-performance elimination of co-existing heavy metals and organic pollutants in water.

7.
Ecotoxicol Environ Saf ; 202: 110854, 2020 Jun 22.
Artigo em Inglês | MEDLINE | ID: mdl-32585484

RESUMO

Atrazine as a kind of herbicide could cause damage to the sensitive plants. Though plant growth promoting rhizobacteria (PGPR) have been proven with the potential to enhance the resistance of plants against various abiotic stresses, whether it could alleviate phytotoxicity caused by atrazine is sill unclear. In present study, the effects of strain Pseudomonas chlororaphis PAS18, a kind of PGPR enable to produce indole-3-acetic acid (IAA), on the growth and physiological responses of Pennisetum americanum (L.) K.Schum seedlings were investigated under three different levels (0, 20 and 100 mg kg-1) of atrazine in pot experiment. The results suggest that strain PAS18 could alleviate the growth and physiological interference caused by 20 mg kg-1 of atrazine. Physiological analysis showed strain PAS18 could further decrease the damaged extent of photosystem II, superoxide radical level and malondialdehyde content of test plant via up-regulating psbA expression, enhancing superoxide dismutase activity and reducing atrazine accumulation in the test plant. Moreover, ion flux measurements suggest that IAA could alleviate the Ca2+ exflux state of the test plant which caused by atrazine stress. Hence, it is plausible that strain PAS18 could alleviate atrazine-induced stress to P. americanum by enhancing the photosystem II repair and antioxidant defense ability as well as balancing the Ca2+ flux.

8.
Magn Reson Imaging ; 70: 145-154, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32380160

RESUMO

The optics of the ocular lens are determined by its geometry (shape and volume) and its inherent gradient of refractive index (water to protein ratio), which are in turn maintained by unique cellular physiology known as the lens internal microcirculation system. Previously, magnetic resonance imaging (MRI) has been used on ex vivo organ cultured bovine lenses to show that pharmacological perturbations to this microcirculation system disrupt ionic and fluid homeostasis and overall lens optics. In this study, we have optimised in vivo MRI protocols for use on wild-type and transgenic mouse models so that the effects of genetically perturbing the lens microcirculation system on lens properties can be studied. In vivo MRI protocols and post-analysis methods for studying the mouse lens were optimised and used to measure the lens geometry, diffusion, T1 and T2, as well as the refractive index (n) calculated from T2, in wild-type mice and the genetically modified Cx50KI46 mouse. In this animal line, gap junctional coupling in the lens is increased by knocking in the gap junction protein Cx46 into the Cx50 locus. Relative to wild-type mice, Cx50KI46 mice showed significantly reduced lens size and radius of curvature, increased T1 and T2 values, and decreased n in the lens nucleus, which was consistent with the developmental and functional changes characterised previously in this lens model. These proof of principle experiments show that in vivo MRI can be applied to transgenic mouse models to gain mechanistic insights into the relationship between lens physiology and optics, and in the future suggest that longitudinal studies can be performed to determine how this relationship is altered by age in mouse models of cataract.

9.
Ecotoxicology ; 29(6): 669-675, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32333253

RESUMO

Crab is an important benthonic animal in mangrove ecosystem, however, the potential function of crabs on nitrogen (N) transformation in mangrove ecosystems is still poorly understood. The present study aimed to explore the potential effect of crab burrows on nitrification/denitrification within the sediments. The results showed that the presence of crab burrows could directly promote soil nitrification, the regions within more crab burrows appeared to possess higher nitrification. Higher AOA and AOB gene copies were also observed in the sediments surrounding crab burrows than those in the sediments without crab burrow. On the contrary, lower nirS copies, a denitrification related gene, were detected in the sediments surrounding crab burrows. In summary, the present study proposed new evidences of nitrification enhancement deriving by crabs, the presence of crabs might be significant in alleviating nitrification inhibition and benefits the growth of mangroves under tidal flooding.


Assuntos
Braquiúros/fisiologia , Ciclo do Nitrogênio , Nitrogênio , Poluição da Água , Áreas Alagadas , Animais , Monitoramento Ambiental , Sedimentos Geológicos
10.
Medicine (Baltimore) ; 99(16): e19687, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32311943

RESUMO

BACKGROUND: Problem-based learning (PBL) combined lecture based learning (LBL) has been a trend adopted as a new medical pedagogical approach in Chinese medical teaching. This study aims to evaluate the impacts of hybrid PBL and LBL pedagogy compared with LBL teaching method on the learning achievements of clinical curriculum for Chinese medicine students. METHODS: Randomized controlled trials (RCTs) were identified through a systematic literature search of electronic databases and article references up to June 2019. PubMed, EBSCO, Web of Science, Cochrane Library, Wanfang Database, CNKI, and China Biology Medicine database (CBM) were searched. End points included knowledge scores, skill scores, medical writing scores, comprehensive ability scores and teaching satisfaction. RESULTS: Totally 20 randomized controlled studies were finally included and all Chinese literatures, involving 1817 patients. Compared with traditional LBL pedagogy, hybrid PBL and LBL pedagogy significantly increased the clinical theoretical knowledge assessment score (RR = 4.84, 95% CI: 2.92∼6.76, P < .00001), clinical skills assessment score (RR = 1.60, 95% CI: 1.39∼1.81, P < .00001), comprehensive ability score (RR = 9.13, 95% CI: 8.42∼9.84, P < .00001) and teaching satisfaction (RR = 2.58, 95% CI: 1.84∼3.62, P < .00001), The meta-regression results showed that expertise-level of students and course type were the factors that caused heterogeneity. CONCLUSIONS: Hybrid PBL and LBL pedagogy integrates the advantages of conventional teaching methods and novel teaching methods, remarkably improves the teaching efficacy, demonstrates easy acceptance by students, and meets the demand of modern medical education, so it is an effective approach to cultivate the medical talents with an ability of innovative thinking and can be advocated and popularized as a teaching means.


Assuntos
Educação Médica/métodos , Aprendizagem Baseada em Problemas/métodos , China , Competência Clínica , Humanos , Aprendizagem , Ensaios Clínicos Controlados Aleatórios como Assunto , Estudantes de Medicina/psicologia
11.
Eur J Radiol ; 126: 108965, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32268245

RESUMO

PURPOSE: To explore the application value of conventional ultrasound and real-time shear wave elastography (SWE) to the tibial nerve (TN) and the common peroneal nerve (CPN) in diabetic peripheral neuropathy (DPN). MATERIALS AND METHODS: Thirty-three healthy volunteers, 33 diabetic patients without DPN, and 30 diabetic patients with DPN were enrolled in this study. The anteroposterior diameter (APD), the cross-sectional area (CSA), and the perimeter of the TN and the CPN were measured by conventional ultrasound, and the stiffness of the nerves was measured by SWE. RESULTS: The conventional ultrasound parameters and stiffness of the TN in patients with DPN were significantly larger than those of the other two groups (P < 0.01). The conventional ultrasound parameters of the CPN were significantly higher in patients with DPN than in the other two groups (P < 0.01).The patients with DPN demonstrated a greater stiffness of the CPN compared to the control group (P < 0.05). The comparison of all parameters for the left and right TNs and CPNs among the three groups showed no significant difference. The area under the curve (AUC) of TN stiffness for the diagnosis of DPN was significantly greater than that of conventional ultrasound parameters. CONCLUSION: The conventional ultrasound parameters and the stiffness of the TN and the CPN were significantly higher in patients with DPN. The stiffness of the TN could better diagnose DPN than conventional ultrasound parameters. In short, conventional ultrasound and SWE of nerves are of good application value in the diagnosis of DPN.

12.
Environ Res ; 184: 109260, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32113024

RESUMO

Atrazine is a widely used herbicide whose presence poses a potential threat to agriculture and human living environment. This work studied the degradation performances and mechanisms of zero-valent iron and biochar composite (ZVI/BC) activated persulfate (PS) for atrazine. The results showed that the removal percentage of atrazine reached 73.47% within 30 min. Furthermore, the optimal parameters (175 mg/L ZVI/BC, 2 mM PS and initial pH of 3) were obtained through response surface methodology. Meanwhile, the high atrazine removal percentage (83.77%) was obtained under the optimal conditions. Radical quenching studies and electron spin resonance revealed that active substances produced during PS activation, as well as that SO4·- and HO· were dominant active species for the atrazine degradation. According to iron corrosion products and XPS analysis, the reaction mechanism of ZVI/BC-PS system was proposed as that ZVI loaded on the composites further activated PS to produce SO4·- and HO· which accompany with the valent changing of iron and finally causing degradation of atrazine. In addition, the degradation pathways of atrazine in ZVI/BC-PS system included dealkylation, alkyl oxidation and dechlorination-hydroxylation by the results of GC-MS and LC-MS. These findings demonstrated that ZVI/BC activated persulfate may be an efficient technique for the degradation of atrazine.

13.
J Neurooncol ; 147(3): 547-555, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32215786

RESUMO

INTRODUCTION: Glioblastoma multiforme (GBM) is a deadly brain tumor with a short expected median survival, despite current standard-of-care treatment. We explored the combination of intermediate stereotactic dose radiation therapy and immune checkpoint inhibitor therapy as a novel treatment strategy for GBM. METHODS: Glioma xenograft-bearing mice were exposed to high dose brain-directed radiation (10 Gy single exposure) as well as mouse anti-PD-1 antibody. The tumor-bearing animals were randomized to four groups: no treatment, radiation alone, anti-PD-1 alone, and radiation + anti-PD-1. Survival was followed, and tumor growth was monitored using MRI. Immunohistochemistry, gene expression arrays, and flow cytometry were used to characterize the treatment-induced effects. Pharmacologic inhibitors of T-lymphocytes, bone marrow derived macrophages, and microglia were used to assess the respective roles of different immune populations in observed treatment effects. RESULTS: We found the combined treatment with high dose radiation and immunotherapy to be highly effective with a 75% complete pathologic response and dramatically improved survival outcomes. We found both CD8+ T-cells and macrophages to be necessary for the full effect of combined therapy, with T lymphocytes appearing to play a role early on and macrophages mediating a later phase of the combined treatment effect. Radiation treatment appeared to trigger macrophage repolarization, increasing M1/M2 ratio. CONCLUSIONS: These findings point to a novel immunologic mechanism underlying the interaction between radiotherapy and immunotherapy. They also provide the basis for clinical investigation of immunogenic dose radiation in combination with immune checkpoint blockade as a potential treatment approach for newly diagnosed high grade gliomas.

15.
Autophagy ; : 1-16, 2020 Jan 19.
Artigo em Inglês | MEDLINE | ID: mdl-31941401

RESUMO

Macroautophagy (autophagy) is driven by the coordinated actions of core autophagy-related (Atg) proteins. Atg8, the core Atg protein generally considered acting most downstream, has recently been shown to interact with other core Atg proteins via their Atg8-family-interacting motifs (AIMs). However, the extent, functional consequence, and evolutionary conservation of such interactions remain inadequately understood. Here, we show that, in the fission yeast Schizosaccharomyces pombe, Atg38, a subunit of the phosphatidylinositol 3-kinase (PtdIns3K) complex I, interacts with Atg8 via an AIM, which is highly conserved in Atg38 proteins of fission yeast species, but not conserved in Atg38 proteins of other species. This interaction recruits Atg38 to Atg8 on the phagophore assembly site (PAS) and consequently enhances PAS accumulation of the PtdIns3K complex I and Atg proteins acting downstream of the PtdIns3K complex I, including Atg8. The disruption of the Atg38-Atg8 interaction leads to the reduction of autophagosome size and autophagic flux. Remarkably, the loss of this interaction can be compensated by an artificial Atg14-Atg8 interaction. Our findings demonstrate that the Atg38-Atg8 interaction in fission yeast establishes a positive feedback loop between Atg8 and the PtdIns3K complex I to promote efficient autophagosome formation, underscore the prevalence and diversity of AIM-mediated connections within the autophagic machinery, and reveal unforeseen flexibility of such connections.Abbreviations: AIM: Atg8-family-interacting motif; AP-MS: affinity purification coupled with mass spectrometry; Atg: autophagy-related; FLIP: fluorescence loss in photobleaching; PAS: phagophore assembly site; PB: piggyBac; PE: phosphatidylethanolamine; PtdIns3K: phosphatidylinositol 3-kinase; PtdIns3P: phosphatidylinositol 3-phosphate.

16.
J Hazard Mater ; 387: 121718, 2020 04 05.
Artigo em Inglês | MEDLINE | ID: mdl-31771887

RESUMO

Herein, microwave-functionalized cellulose derived from rice husk was cost-effectively prepared and employed for Pb(II), Cd(II) and Ni(II) elimination in mono- and multi-component systems. Benefiting from microwave irradiation, the functionalized process was achieved in 6.5 min and the resultant RHMW-X possessed remarkably high adsorption capacities of 295.20 mg/g for Pb(II), 151.51 mg/g for Cd(II) and 72.80 mg/g for Ni(II) within the equilibrium time of 30 min. Noticeably, the metal ions adsorption rate and capacity in binary and ternary systems were lower than that of unary systems. The coexistence of Cd(II) and Ni(II) significantly slowed down the Pb(II) adsorption in binary and ternary systems, while Pb(II) exhibited the most obvious influence on the metal ions uptake in the multi-component systems. FT-IR and XPS results revealed that both ion exchange and chelation were functioned in the metal ions uptake, while physical interaction was also involved in the adsorption process. Moreover, the RHMW-X possessed favorable recyclability with slight adsorption efficiency decline during five cycles in different systems. Particularly, the RHMW-X could effectively purify actual industrial wastewater containing Pb(II), Cd(II) and Ni(II) for meeting regulatory requirements. This work facilitates the omnidirectional improvement of adsorbents for the de-pollution of practical heavy metals wastewater.

17.
Chemosphere ; 238: 124594, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31445334

RESUMO

Enhancing the biodegradation efficiency of atrazine, a kind of commonly applied herbicide, has been attracted much more concern. Here, Zn2+ which has long been considered essential in adjusting cell physiological status was selected to investigate its role on the biodegradation of atrazine by Arthrobacter sp. DNS10 as well as the transmembrane transport of atrazine during the biodegradation period. The results of gas chromatography showed that the atrazine removal percentages (initial concentration was 100 mg L-1) in 0.05 mM Zn2+ and 1.0 mM Zn2+ treatments were 94.42% and 86.02% respectively at 48 h, while there was also 66.43% of atrazine left in the treatment without exogenous Zn2+ existence. The expression of atrazine chlorohydrolase gene trzN in the strain DNS10 cultured with 0.05 mM and 1.0 mM Zn2+ was 7.30- and 4.67- times respectively compared with that of the non-zinc treatment. In addition, the flow cytometry test suggests that 0.05 mM of Zn2+ could better adjust the membrane permeability of strain DNS10, meanwhile, the amount of atrazine accumulation in the strain DNS10 co-cultured with this level Zn2+ was 2.21 times of that of the strain without Zn2+. This study may facilitate a better understanding of the mechanisms that exogenous Zn2+ enhances the biodegradation of atrazine by Arthrobacter sp. DNS10.


Assuntos
Arthrobacter/metabolismo , Atrazina/análise , Herbicidas/análise , Hidrolases/biossíntese , Zinco/farmacologia , Biodegradação Ambiental/efeitos dos fármacos , Transporte Biológico/fisiologia , Hidrolases/genética , Permeabilidade/efeitos dos fármacos , Microbiologia do Solo
18.
Int J Ophthalmol ; 12(10): 1634-1636, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31637201

RESUMO

To investigate the possible correlation between obstructive sleep apnea (OSA) and central retinal vein occlusion (CRVO). Thirty consecutive patients with a recent (<3mo) CRVO and an age- and sex-matched group of 30 control subjects were recruited. All subjects underwent full-night polysomnography to measure apnea-hypopnea index (AHI) and oxygen desaturation index (ODI). The average AHI and ODI were significantly higher in CRVO patients (AHI: 13.86±8.63, ODI: 9.21±4.47) than in control subjects (AHI: 8.51±6.36, ODI: 5.87±3.18; P=0.008 and 0.001 respectively). Additionally, the AHI was positively correlated with body mass index (BMI; r=0.476, P=0.017) and ODI (r=0.921, P<0.01) in both CRVO and control subjects. According to AHI scores, twenty-two (73.33%) CRVO patients had OSA and 12 (40.00%) control subjects had OSA, a difference that was statistically significant (P=0.019). OSA may be a risk factor for or a trigger of CRVO development.

19.
Chemosphere ; 234: 917-924, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31519100

RESUMO

Nickel, massively used in plating industry but detrimental to ecosystem, tends to form stable complexes with organic additives in industrial effluents. Currently, most of the available processes aim at water decontamination from free toxic metal ions and thus, could not effectively remove nickel-carboxyl complexes from water. Herein, we employed a proprietary combined process Fe(III)/UV/NaOH, namely Fe(III) displacement and UV irradiation followed by alkaline precipitation, to validate its feasibility on the efficient removal of nickel-carboxyl complexes from synthetic and authentic effluents. Fe(III)/UV/NaOH outperformed other commonly used processes including NaOH precipitation, UV/NaOH, Fe(III) coagulation, and Fenton/NaOH. Each unit of the combined process was optimized, and the underlying mechanism was elucidated. Fe(III) displacement favored the stoichiometric release of free nickel ions and formation of Fe(III)-carboxyl complexes, which could be decarboxylated via ligand-metal charge transfer under UV irradiation. The precipitation unit aims at simultaneously removing the released Ni along with Fe species. Attractively, the presence of other organic species (ethylene glycol, ethyl acetate and humic acid) and anions (chloride and sulfate) exerted very slight effect on the final Ni removal, whereas greatly adverse effect occurred on the Fenton process under similar conditions. The feasibility of the combined process was validated by testing on an authentic electroplating effluent, resulting in the residual Ni below 0.1 mg/L, the most stringent discharge standard for Ni in electroplating effluent in China.


Assuntos
Compostos Férricos/química , Níquel/química , Poluentes Químicos da Água/química , Purificação da Água/métodos , China , Ecossistema , Galvanoplastia , Hidróxido de Sódio , Sulfatos , Raios Ultravioleta , Água , Poluentes Químicos da Água/análise
20.
ACS Nano ; 13(9): 10419-10433, 2019 09 24.
Artigo em Inglês | MEDLINE | ID: mdl-31430127

RESUMO

Radio- and photodynamic therapies are the first line of cancer treatments but suffer from poor light penetration and less radiation accumulation in soft tissues with high radiation toxicity. Therefore, a multifunctional nanoplatform with diagnosis-assisted synergistic radio- and photodynamic therapy and tools facilitating early prognosis are urgently needed to fight the war against cancer. Further, integrating cancer therapy with untargeted metabolomic analysis would collectively offer clinical pertinence through facilitating early diagnosis and prognosis. Here, we enriched scintillation of CeF3 nanoparticles (NPs) through codoping Tb3+ and Gd3+ (CeF3:Gd3+,Tb3+) for viable clinical approach in the treatment of deep-seated tumors. The codoped CeF3:Gd3+,Tb3+ scintillating theranostic NPs were then coated with mesoporous silica, followed by loading with rose bengal (CGTS-RB) for later computed tomography (CT)- and magnetic resonance image (MRI)-guided X-ray stimulated synergistic radio- and photodynamic therapy (RT+XPDT) using low-dose, one-time X-ray irradiation. The results corroborated an efficient tumor regression with synergistic RT+XPDT relative to single RT. Global untargeted metabolome shifts highlighted the mechanism behind this efficient tumor regression using RT, and synergistic RT+XPDT treatment is due to the starvation of nonessential amino acids involved in protein and DNA synthesis and energy regulation pathways necessary for growth and progression. Our study also concluded that tumor and serum metabolites shift during disease progression and regression and serve as robust biomarkers for early assessment of disease state and prognosis. From our results, we propose that codoping is an effective and extendable technique to other materials for gaining high optical yield and multifunctionality and for use in diagnostic and therapeutic applications. Critically, the integration of multifunctional theranostic nanomedicines with metabolomics has excellent potential for the discovery of early metabolic biomarkers to aid in better clinical disease diagnosis and prognosis.

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