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1.
Mol Med Rep ; 24(6)2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34651663

RESUMO

Diffuse large B­cell lymphoma (DLBCL) is the most common type of non­Hodgkin lymphoma worldwide. Several studies have indicated that Homo sapiens (hsa)­microRNA (miR)­429 exerts a tumor­suppressive effect on a variety of malignant tumors. To the best of our knowledge, the molecular function and mechanism of action of hsa­miR­429 in DLBCL have not been evaluated to date. The present study demonstrated that the expression of hsa­miR­429 in DLBCL cells was significantly reduced. hsa­miR­429 inhibited the proliferation of the DLBCL cell lines, SUDHL­4 and DB, and promoted apoptosis. A dual luciferase reporter assay was used to demonstrate that chromobox 8 (CBX8) was the target gene of hsa­miR­429. Overexpression of CBX8 promoted the proliferation of SUDHL­4 and DB cells and inhibited apoptosis, thereby playing a cancer­promoting role. Transfection of hsa­miR­429 mimic into DB cells overexpressing CBX8 antagonized the effect of CBX8 on the proliferation of DB cells. Moreover, the apoptotic rate was increased in DB cells overexpressing CBX8 and transfected with hsa­miR­429 mimic, while the proportion of cells in the G2/M phase was significantly reduced. These results demonstrated the antagonistic effect of hsa­miR­429 on the oncogenic function of CBX8. Therefore, in DLBCL, the tumor suppressor effect of hsa­miR­429 may be achieved by targeted downregulation of CBX8, suggesting that hsa­miR­429 may be used as a diagnostic marker and a potential nucleic acid drug for DLBCL. CBX8 may also represent an effective therapeutic target for DLBCL.

2.
Small ; : e2103830, 2021 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-34643046

RESUMO

All-solid-state lithium batteries (ASSLBs) adopting sulfide electrolytes and high-voltage layered oxide cathodes have moved into the mainstream owing to their superior safety and immense potential in high energy density. However, the poor electrochemical compatibility between oxide cathodes and sulfide electrolytes remains a challenge for high-performance ASSLBs. In this study, a nanoscale Li1.4 Al0.4 Ti1.6 (PO4 )3 (LATP) phosphate coating is reasonably constructed on the surface of single-crystal LiNi0.6 Co0.2 Mn0.2 O2 particles to achieve cathode/electrolyte interfacial stability. The conformal LATP layer with inherent high-voltage stability can effectively suppress the oxidation decomposition of the electrolyte and demonstrate chemical inertness to both the oxide cathode and Li10 SnP2 S12 electrolyte. ASSLBs with an LATP-modified cathode exhibited a high initial discharge capacity (152.1 mAh g-1 ), acceptable rate capability, and superior cycling performance with a capacity retention of 87.6% after 100 cycles at 0.1 C. Interfacial modification is an effective approach for achieving high-performance sulfide-based ASSLBs with superior interfacial stability.

3.
Neurol Sci ; 2021 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-34669083

RESUMO

Early studies have indicated that the risk of migraine is contributed by both genetic and environmental factors. We aimed to evaluate the association between the risk of migraine and genetic polymorphisms in the ANKDD1B gene in a large sample of Chinese Han populations. A total of 882 patients with MO and 1,784 age-matched controls were recruited. A list of 12 tag SNPs located within the ANKDD1B gene region was genotyped. Distributions of SNP genotypes and alleles between patients and controls were examined to investigate the associations between the risk of migraine and genetic polymorphisms. The GTEx database was used to examine the effects of the significant SNPs on gene expressions. A stop-gain SNP, rs34358, was discovered to be significantly related with the risk of migraine (χ2 = 25.02, P = 5.66 × 10-7). The A allele of this SNP was significantly associated with a decreased risk of migraine (OR [95% CI] = 0.73 [0.65-0.83]). A dose-dependent pattern was identified in the genotypic analyses. The OR with 95% confidence interval for genotype AA versus GG was 0.55 [0.42-0.72], while for AG versus GG it was 0.74 [0.62-0.88]. Further bioinformatics analysis showed multiple significant signals (20 out of 47) for the association between SNP rs34358 and gene expression levels of ANKDD1B. In conclusion, we have provided population-based evidence for the association between genetic polymorphisms of the ANKDD1B gene and the risk of migraine. A protein-truncating variant was significantly associated with a decreased risk of migraine in the samples recruited from the Chinese Han population.

4.
J Hazard Mater ; 423(Pt A): 127043, 2021 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-34479084

RESUMO

Bioremediation by phosphate-solubilizing bacteria (PSB) has attracted extensive attentions due to its economical and eco-friendly properties for lead (Pb) passivation in soil. Herein, bone char (BC) supported biochemical composite (CFB1-P) carrying advantages of BC, PSB, iron sulfide (FeS) and carboxymethyl cellulose (CMC) was designed and applied to Pb passivation. The composite at a mass ratio of BC:CMC:FeS = 1:1:1 possessed high passivation efficiency (65.47%), and has been demonstrated to offer appropriate habitat environment for PSB to defend against Pb(II) toxicity, thus enhancing the phosphate-solubilizing amount of PSB to 140.72 mg/L for passivating Pb(II). Batch experiments showed that the CFB1-P possessed excellent adsorption properties with maximal monolayer Pb(II) uptake of 452.99 mg/g during an extensive pH range of 2.0-6.0. Furthermore, by applying CFB1-P dosage of 3% into Pb-contaminated soil, the labile Pb fractions were reduced from 29.05% to 6.47% after simulated remediation of 10 days, and converted into steady fractions. The CFB1-P was demonstrated to achieve high Pb(II) passivation through combined functions of chemical precipitation, complexation, electrostatic attraction and biomineralization, accompanied by the formation of more stable crystal structures, for instance, Pb5(PO4)3OH, Pb3(PO4)2 and PbS. These results suggested CFB1-P as a potential alternative for efficient remediation of Pb-contaminated soil.

5.
Bioresour Technol ; 340: 125692, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34358982

RESUMO

Magnetic porous water hyacinth-derived biochar (MPBCMW3) was synthesized via two-step Microwave (MW)-assisted processes. Characterization results not only testified high specific surface area (2097.50 m2/g) of the MPBCMW3 assisted by MW-assisted pyrolysis, but also revealed its favorable magnetism derived from MW-assisted hydrothermal process. The MPBCMW3 possessed pH-dependent monolayer adsorption capacities of 202.61 and 202.62 mg/g for Cr(VI) and TC with quick attainments of uptake equilibrium within 150 and 200 min. Moreover, the Cr(VI) and TC uptake were substantially steady under the interference from multifarious co-existing ions with slight decline after three adsorption-desorption cycles. Furthermore, the MPBCMW3 was demonstrated to achieve excellent Cr(VI) binding primarily through complexation, electrostatic interaction, reduction and ion exchange, while presenting outstanding TC removal via pore filling, π-π stacking, hydrogen bonding force, electrostatic interaction and complexation. All these findings suggested the MPBCMW3 synthesized by MW-assisted processes as an excellent adsorbent for purification of Cr(VI) and TC-contaminated water.


Assuntos
Eichhornia , Poluentes Químicos da Água , Adsorção , Carvão Vegetal , Cromo , Cinética , Micro-Ondas , Porosidade , Pirólise , Tetraciclina , Água , Poluentes Químicos da Água/análise
6.
ChemSusChem ; 14(20): 4454-4465, 2021 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-34323383

RESUMO

A two-dimensional electron-rich fused-ring moiety (ClBDSe) based on benzo[1,2-b:4,5-b']diselenophene is synthesized. Three copolymers (PBDT-Se, PBDSe-T, and PBDSe-Se) are obtained by manipulating the connection types and number of selenophene units on the conjugated main chains with two 2D fused-ring units and two different π-bridges, respectively. In comparison with PBDT-Se and PBDSe-Se, PBDSe-T with benzo[1,2-b:4,5-b']diselenophene unit and thiophene π-bridge exhibits the deepest HOMO energy level and the strongest crystallinity in neat films. The PBDSe-T:Y6 blend film exhibits the best absorption complementarity, the most distinctive face-on orientation with proper phase separation, the highest carrier mobilities, and the lowest charge recombination among three blend films. Finally, the PBDSe-T:Y6-based device delivers an impressive power conversion efficiency (PCE) of 14.50 %, which is higher than those of PBDT-Se:Y6 and PBDSe-Se:Y6. Moreover, a decent open-circuit voltage (Voc ) of 0.89 V with a remarkably small energy loss of 0.44 eV is achieved for PBDSe-T:Y6. The efficiency of 14.50 % is the highest value for selenophene-containing copolymer-based binary organic solar cells (OSCs). This study provides evidence that introduction of 2D-benzo[1,2-b:4,5-b']diselenophene as a fused electron-rich unit with π-bridging into copolymeric donors is a valid strategy for providing high Voc and excellent PCE simultaneously in selenophene-based OSCs.

7.
J Environ Manage ; 297: 113220, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34274768

RESUMO

The pollutants in soils with different physicochemical properties usually exhibit various phytotoxicity due to their diverse environmental behavior. However, it is not clear that some components of dissolved organic matter (DOM) in soil affect the bioavailability of pollutants. In this study, the different phytotoxicity of atrazine in two different soils, black soil (BS) and yellow brown earth (YBE), to soybeen seedlings was investigated, and the potential mechanism were further discussed by examining the adsorption properties of atrazine with BS and YBE as well as the fluorescent characteristic variation of these two kinds of DOM extracted from BS and YBE during binding with atrazine. The results suggest that atrazine showed different phytotoxicity to soybean seedlings grown in BS and YBE. Specifically, the EC50 of atrazine on plant height and root length of soybean seedling in BS were 23.44 mg kg-1 and 54.29 mg kg-1, while those in YBE were 12.79 mg kg-1 and 21.48 mg kg-1. The adsorption amounts of BS and YBE was 41.56 mg kg-1 and 32.95 mg kg-1. In addition, 3D-EEM showed that humic acid-like substances were the main components of DOM in the two soils. With the increase of atrazine concentration, the fluorescence of these two kind of DOM was gradually quenched, but DOM-BS had a faster quenching rate. FT-IR showed that DOM-BS contained more polysaccharides and richer lipophilic groups. Meanwhile, the results of PARAFAC found that DOM-BS was easier to combine with atrazine. The findings of this study are significant to reveal phytotoxicity of atrazine in BS and YBE. The above results are helpful to better understand the components of DOM in different soils and their different role in regulating the environmental behavior and phytotoxicity of organic pollutants.


Assuntos
Atrazina , Poluentes do Solo , Adsorção , Atrazina/toxicidade , Substâncias Húmicas/análise , Solo , Poluentes do Solo/análise , Poluentes do Solo/toxicidade , Soja , Espectroscopia de Infravermelho com Transformada de Fourier
8.
Opt Express ; 29(13): 20322-20335, 2021 Jun 21.
Artigo em Inglês | MEDLINE | ID: mdl-34266124

RESUMO

In this paper, a continuous optical zoom microscopy imaging system based on liquid lenses is proposed. Compared with traditional microscopes, which have discrete magnification, requiring manual conversion of the objective lens to change the magnification, the proposed microscope can continuously change the magnification of the targets in real-time. An adaptive zoom microscope, a liquid lens driving board, a microscope bracket, an adjustable three-dimensional stage and a light source are stacked to form the main framework of the continuous optical zoom microscopy imaging system. The adaptive zoom microscope which is composed of four electrowetting liquid lenses and six glass lenses form the main imaging element of the microscope. By changing the driving voltage which is applied to the four liquid lenses, the focal length of the liquid lenses can be modulated to achieve continuous zooming. By contrast, in traditional microscopes, the zooming process can only be achieved by rotating the eyepieces at different magnifications. At a fixed working distance, the magnification of the proposed microscope can change continuously from ∼9.6× to ∼22.2× with a response time of ∼50ms. Moreover, an axial depth scanning of ∼1000µm can be achieved without any mechanical movement. Our experiments proved that the microscope has stable performance and high consistency during zooming. Therefore, the proposed microscope has obvious advantages over the traditional microscopes in observing dynamic samples with different magnifications and can be commercialized for further expanding the applications in biochemical and pathological analysis.

9.
Ecotoxicology ; 2021 Jul 16.
Artigo em Inglês | MEDLINE | ID: mdl-34269924

RESUMO

In this study, Illumina MiSeq sequencing of the 16 S rRNA gene was used to describe the bacterial communities in the South China Sea (SCS) during the southwest monsoon period. We targeted different regions in the SCS and showed that bacterial community was driven by the effects of the river, upwelling, and mesoscale eddy through changing the environmental factors (salinity, temperature, and nutrients). Distinct bacterial communities were observed among different chemical conditions, especially between the estuary and the open sea. The abundance of Burkholderiales, Frankiales, Flavobacteriales, and Rhodobacterales dominated the estuary and its adjacent waters. Bacteria in cyclonic eddy were dominated by Methylophilales and Pseudomonadales, whereas Prochlorococcus, SAR11 clade, and Oceanospirillales had relatively high abundance in the anticyclonic eddy. Overall, the abundance of specific phylotypes significantly varied among samples with different chemical conditions. Chemical conditions probably act as a driver that shapes and controls the diversity of bacteria in the SCS. This study suggests that the interaction between microbial and environmental conditions needs to be further considered to fully understand the diversity and function of marine microbes.

10.
Artigo em Inglês | MEDLINE | ID: mdl-34292143

RESUMO

A novel Gram-stain-positive, catalase-positive, oxidase-negative, aerobic, non-motile, rod-shaped bacterium, designated strain YIM M12148T, was isolated from a marine sediment sample collected from the Indian Ocean. The strain grew optimally at 28 °C, pH 8.0 and in the presence of 1-3 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM M12148T belongs to the genus Gulosibacter, with the highest sequence similarity to Gulosibacter faecalis NBRC 15706T (96.12 %). The cell-wall sugars of strain YIM M12148T were rhamnose, ribose, glucose and mannose. The predominant isoprenoid quinones were MK-8 and MK-9. The polar lipids consisted of major amounts of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unknown phospholipid and one unknown lipid. Major fatty acids (>5 % of the total) of the novel isolate were anteiso-C15 : 0, iso-C15 : 0, iso-C13 : 0 and anteiso-C13 : 0. The genomic DNA G+C content of strain YIM M12148T was 67.15 mol%. On the basis of genotypic and phenotypic data, it is apparent that strain YIM M12148T represents a novel species of the genus Gulosibacter, for which the name Gulosibacter sediminis sp. nov. is proposed. The type strain is YIM M12148T (=KCTC 29660T=DSM 29154T).


Assuntos
Actinobacteria/classificação , Sedimentos Geológicos/microbiologia , Filogenia , Água do Mar/microbiologia , Actinobacteria/isolamento & purificação , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Oceano Índico , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
11.
New Phytol ; 232(1): 440-455, 2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-34165797

RESUMO

Accurate and high-throughput phenotyping of the dynamic response of a large rice population to drought stress in the field is a bottleneck for genetic dissection and breeding of drought resistance. Here, high-efficiency and high-frequent image acquisition by an unmanned aerial vehicle (UAV) was utilized to quantify the dynamic drought response of a rice population under field conditions. Deep convolutional neural networks (DCNNs) and canopy height models were applied to extract highly correlated phenotypic traits including UAV-based leaf-rolling score (LRS_uav), plant water content (PWC_uav) and a new composite trait, drought resistance index by UAV (DRI_uav). The DCNNs achieved high accuracy (correlation coefficient R = 0.84 for modeling set and R = 0.86 for test set) to replace manual leaf-rolling rating. PWC_uav values were precisely estimated (correlation coefficient R = 0.88) and DRI_uav was modeled to monitor the drought resistance of rice accessions dynamically and comprehensively. A total of 111 significantly associated loci were detected by genome-wide association study for the three dynamic traits, and 30.6% of them were not detected in previous mapping studies using nondynamic drought response traits. Unmanned aerial vehicle and deep learning are confirmed effective phenotyping techniques for more complete genetic dissection of rice dynamic responses to drought and exploration of valuable alleles for drought resistance improvement.


Assuntos
Oryza , Secas , Variação Genética , Estudo de Associação Genômica Ampla , Oryza/genética , Melhoramento Vegetal
13.
Saudi J Biol Sci ; 28(6): 3254-3261, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-34121863

RESUMO

This study investigated the influence of the exogenous application of vitamin B2 (VB2), B12 (VB12), biotin (VH), and nicotinic acid (VPP) on oxygen production in maize (Zea mays L.) seedlings at 5 °C for day 1, 3, 5 and 7. The seeds were soaked in VB2, VB12, VH, and VPP solutions for 24 h at the concentration of 100 mg/L, and control was soaked in distilled water. A total of 50 seeds were used for each treatment in germination boxes was repeated three times. The germination box was placed in a hypothermic incubator for 1, 3, 5, and 7 days in the dark at 5 °C, then moved to a plant growth room and kept for seven days. Compared with the VH and VPP treatments, the VB2 and VB12 treatments had higher thiobarbituric acid reactive substances, proline, and soluble sugars. The VB2 and VB12 treatments also increased the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) than other treatments. The VB2 and VB12 treatments reduced the contents of hydrogen peroxide (H2O2 -), superoxide anion (O2 -), and the damage of reactive oxygen species (ROS) to cells, increased the stability of the cell membrane and the content of cell osmoregulation substances. Moreover, VB2 and VB12 had higher seedling growth, germination rate, and index. Treatments VB2 and VB12 could promote maize seed germination and growth under low-temperature stress. Exogenous vitamins in crop production can be a valuable tool for protecting plants against low-temperature stress.

14.
ACS Appl Mater Interfaces ; 13(26): 30739-30745, 2021 Jul 07.
Artigo em Inglês | MEDLINE | ID: mdl-34169722

RESUMO

All-solid-state lithium-metal batteries (ASSLMBs) with sulfide electrolytes have attracted attention owing to their superior safety and high energy density. However, interfacial instability of sulfide electrolytes against Li metal still hinders their applications. Herein, F-doping is adopted to optimize the structure of Li10SnP2S12. It is demonstrated that the Li9.95SnP2S11.95F0.05 (LSPSF) electrolyte exhibits a high ionic conductivity of 6.4 mS cm-1 because of F-doping, which can reduce the impurity Li2SnS3 and generate Li+ vacancies. In addition, the Li6PS5I (LPSI) glass-ceramic interlayer is employed to enhance the interfacial stability between the sulfide electrolyte and Li metal by restraining the reduction of Sn4+ cations, as indicated by X-ray photoelectron spectroscopy (XPS). As a result, the assembled ASSLMBs with the LPSI interlayer deliver high initial discharge capacity and remarkable cycling stability. This work provides a new design route for manufacturing high-performance ASSLMBs.

15.
Bioresour Technol ; 337: 125428, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34171706

RESUMO

Magnetic hydrochar modified by ß-cyclodextrin (ß-CD) described as ß-CD@MHC was successfully synthesized and applied to simultaneous removal of cadmium (Cd) and anthracene (ANT). Characterizations attested the grafting of ß-CD groups onto ß-CD@MHC with excellent magnetism. Moreover, the ß-CD@MHC could eliminate Cd(II) and ANT during an extensive pH scope, and presented fast adsorption equilibrium in 60 min and 80 min for Cd(II) and ANT, respectively. And the ß-CD@MHC possessed prominent adsorption properties with maximum monolayer binding of 47.28 mg/g for Cd(II), and corresponding heterogeneous uptake of 60.27 mg/g concerning ANT. Furthermore, the ß-CD@MHC could effectively avoid the competitive behavior between Cd(II) and ANT mainly due to complexation and electrostatic attraction effects for capturing Cd(II), and host-guest interaction in the removal of ANT. Additionally, the binding of Cd(II) and ANT onto ß-CD@MHC dropped slightly after stepwise desorption, suggesting the ß-CD@MHC as a high-performance adsorbent for heavy metals and PAHs elimination from wastewater.


Assuntos
Poluentes Químicos da Água , beta-Ciclodextrinas , Adsorção , Antracenos , Cádmio , Cinética , Fenômenos Magnéticos , Água , Poluentes Químicos da Água/análise
16.
ACS Nano ; 2021 Jun 25.
Artigo em Inglês | MEDLINE | ID: mdl-34170115

RESUMO

X-ray-induced photodynamic therapy (XPDT) is overwhelmingly superior in treating deep-seated cancers. However, limitations remain, owing to a combination of the poor scintillation performance of the nanoscintillator, low energy transfer efficiency of the therapeutic nanoplatform, and hypoxic environment presented in the tumor tissue. Collectively, these reduce the curative effect of XPDT. Here, we report a highly efficient, low-dose XPDT realized by systematic optimization from scintillation efficiency, nanoplatform structure, to therapeutic approach. We developed a biocompatible, codoped CaF2 nanoscintillator that emitted sufficiently green radioluminescence that was bright enough to be seen by the naked eye. Using dendrimers as a framework, we built a nanoplatform featuring a dual-core-satellite architecture, which enabled both procedurally and spatially separate dual-loading of therapeutic agents. This strategy allowed for the fabrication of a combined XPDT and antiangiogenic therapy, resulting in a therapeutic system capable of simultaneous tumor attacks. After exposure to ultralow dose radiation, XPDT resulted in marked tumor reduction while the antiangiogenic drug effectively blocked tumor vascularization exacerbated by XPDT-mediated hypoxia, rendering a pronounced synergy effect. This system also showed high biosafety, as the agents adopted had been used clinically and both Ca and F elements were widespread in the human body. Taken together, the findings presented here provided a reference for the construction of complex, multiloading architecture in coordination with structural complexity and functional diversification. This work provided a safer and more robust application of the combined XPDT and antiangiogenesis in future clinical treatment settings.

17.
Epilepsy Res ; 175: 106696, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34186384

RESUMO

PURPOSE: Depression and anxiety are often comorbid in people with epilepsy. Network models consider this comorbidity as an interacting system of depressive and anxiety symptoms. The present study investigates the network structure of depressive and anxiety symptoms in people with epilepsy and aims to identify the central and bridge symptoms to provide suggestions for the prevention of and intervention for depression-anxiety comorbidity in patients with epilepsy. METHODS: A total of 313 patients with epilepsy were enrolled in our study. Anxiety symptoms were evaluated with the Generalized Anxiety Disorder 7-Item questionnaire. Depressive symptoms were evaluated with the Patient Health Questionnaire-9. Network analyses were used for the statistical analysis. RESULTS: The findings indicated that ten edges with the strongest regularized partial correlations existed in the network. Six were among depressive symptoms, such as "sleep difficulties" with "fatigue" and " feeling of worthlessness" with "thoughts of death". Four were among anxiety symptoms, such as "nervousness or anxiety" with "uncontrollable worry" and "uncontrollable worry" with "worry too much". Those strongest edges had no connection linking anxiety and depressive symptoms. The symptoms "depressed or sad mood", "trouble relaxing" and "uncontrollable worry" had the highest strength centrality in the network. The results revealed three bridge symptoms: "psychomotor agitation/retardation", "irritable", and "depressed or sad mood". CONCLUSION: "Feeling of worthlessness" was identified as a key priority due to associations with suicidal ideation. The current study highlighted the critical central symptoms "depressed or sad mood", "trouble relaxing" and "uncontrollable worry" and the critical bridge symptoms "psychomotor agitation/retardation", "irritable", and "depressed or sad mood". Implications for clinical prevention and intervention based on these symptoms are discussed.

19.
Ecotoxicology ; 30(8): 1672-1679, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-33864552

RESUMO

Mangrove reconstruction is an efficient approach for mangrove conservation and restoration. The present study aimed to explore the effects of mangrove reconstruction on sediment properties and bacterial community. The results showed that mangrove restoration greatly promoted sediment fertility, whereas the improvements were more obvious induced by Kandelia obovata when compared to Avicennia marina. In all the samples, the dominant top5 bacterial group were Proteobacteria (48.31-54.52%), Planctomycetes (5.98-8.48%), Bacteroidetes (4.49-11.14%) and Acidobacteria (5.69-8.16%). As for the differences among the groups, the relative abundance of Chloroflexi was higher in the sediments of K. obovata, while Bacteroidetes was more abundant in A. marina group. Furthermore, the two bacterial genera (Rhodoplanes and Novosphingobium) were more dominant in the sediments of K. obovata, while the sediments of A. marina contained higher abundance of Vibrio and Marinobacterium. Besides, bacterial community was highly correlated with mangrove species and sediment property and nutrient status. The results of this study would provide a better understanding of the ecological benefits of mangroves and highlighted the information on biogeochemical processes driven by mangrove restoration and microorganisms.


Assuntos
Avicennia , Rhizophoraceae , Bactérias , Sedimentos Geológicos , Áreas Alagadas
20.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 29(2): 416-421, 2021 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-33812408

RESUMO

OBJECTIVE: To investigate the expression of CD73 in acute myeloid leukemia (AML) patients with NPM1 mutant and wild-type, and to evaluate the therapeutic efficacy and prognosis of CD73 to the AML patients. METHODS: 160 patients with AML treated in our hospital from June 2015 to June 2019 were enrolled, and 40 non-AML bone marrow samples from healthy people were selected as controls during the same period. The expression of CD73 in healthy people, NPM1 mutation and NPM1 wild-type AML patients were compared, and the relationship between the expression of CD73 and its clinicopathological characteristics, as while as efficacy in AML patients were analyzed. The patients were followed up, and the influence of CD73 to the prognosis of different AML patients was analyzed. RESULTS: The positive expression rate of CD73 in AML patients (23.75%) was significantly higher than that in the healthy control group (0.62%), and the positive expression rate of CD73 in AML patients with NPM1 mutation (74.75%) was significantly higher than that with NPM1 wild-type (25.51%) (both P<0.001). AML patients with CD73 positive expression was associated with age, FAB typing, disease risk classification, and NPM1 gene mutation (both P<0.05). The overall survival rate of AML patients with NPM1 gene mutation was 75.98%, which was significantly higher than the patients with NPM1 wild-type (34.68%)(P<0.001), the median survival time of AML patients with NPM1 gene mutation in the CD73+ group was 21 months, which was significantly longer than the patients in the CD73- group (11 months)(P<0.001), the median survival time of AML patients with NPM1 wild-type in the CD73+ group was 13 months, which was significantly shorter than the patients in the CD73- group (18 months) (P<0.001). CONCLUSION: The expression of CD73 was increased in AML patients with NPM1 gene mutation, and CD73 showed different prognostic significance in AML patients with different NPM1 gene mutation. The combination of clinicopathologic features, CD73 expression and NPM1 gene in AML patients is helpful to determine their prognosis and guide the formulation of relevant treatment plans.


Assuntos
Leucemia Mieloide Aguda , Proteínas Nucleares , Humanos , Leucemia Mieloide Aguda/genética , Mutação , Proteínas Nucleares/genética , Prognóstico , Taxa de Sobrevida , Tirosina Quinase 3 Semelhante a fms
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