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1.
Sci Rep ; 10(1): 7909, 2020 May 08.
Artigo em Inglês | MEDLINE | ID: mdl-32385342

RESUMO

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

2.
Nano Lett ; 20(6): 4588-4593, 2020 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-32402200

RESUMO

When surface states (SSs) form in topological insulators (TIs), they inherit the properties of bulk bands, including the electron-hole (e-h) asymmetry but with much more profound impacts. Here via combining magneto-infrared spectroscopy with theoretical analysis, we show that e-h asymmetry significantly modifies the SS electronic structures when interplaying with the quantum confinement effect. Compared with the case without e-h asymmetry, the SSs now bear not only a band asymmetry, such as that in the bulk, but also a shift of the Dirac point relative to the bulk bands and a reduction of the hybridization gap of up to 70%. Our results signify the importance of e-h asymmetry in the band engineering of TIs in the thin-film limit.

3.
Sci Rep ; 10(1): 1511, 2020 01 30.
Artigo em Inglês | MEDLINE | ID: mdl-32001721

RESUMO

Kashmir musk deer Moschus cupreus (KMD) are the least studied species of musk deer. We compiled genetically validated occurrence records of KMD to construct species distribution models using Maximum Entropy. We show that the distribution of KMD is limited between central Nepal on the east and north-east Afghanistan on the west and is primarily determined by precipitation of driest quarter, annual mean temperature, water vapor, and precipitation during the coldest quarter. Precipitation being the most influential determinant of distribution suggests the importance of pre-monsoon moisture for growth of the dominant vegetation, Himalayan birch Betula utilis and Himalayan fir Abies spectabilis, in KMD's preferred forests. All four Representative Concentration Pathway Scenarios result an expansion of suitable habitat in Uttarakhand, India, west Nepal and their associated areas in China in 2050s and 2070s but a dramatic loss of suitable habitat elsewhere (Kashmir region and Pakistan-Afghanistan border). About 1/4th of the current habitat will remain as climate refugia in future. Since the existing network of protected areas will only include a tiny fraction (4%) of the climatic refugia of KMD, the fate of the species will be determined by the interplay of more urgent short-term forces of poaching and habitat degradation and long-term forces of climate change.

4.
Curr Mol Med ; 20(4): 299-306, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31663468

RESUMO

BACKGROUND: Previously, we first identified the human tripartite motifcontaining protein 45 (TRIM45) acts as a novel transcriptional repressor in mitogenactivated protein kinase (MAPK) signaling pathway. After that, the inhibitory role of TRIM45 in the development of tumor was gradually unveiled. However, the function of TRIM45 in the tumorigenesis of lung cancer has not been characterized. METHODS AND RESULTS: In this study, we found that TRIM45 was up-regulated in earlystage human non-small-cell lung cancer (NSCLC) tissues. Overexpression of TRIM45 in lung cancer cells induces G1 arrest and promotes apoptosis, which accompanied by upregulated expression of RB, p16, p53, p27Kip1, and Caspase3 and down-regulated expression of CyclinE1 and CyclinE2. Further detection of the expression of the molecules in the MAPK signaling pathway revealed that overexpression of TRIM45 in lung cancer cells promotes phosphorylated p38 (p-p38) activation and inhibits phosphorylated ERK (p-ERK) activation. In accordance with this, p-p38 is increased while p-ERK is decreased in lung cancer tissues. CONCLUSION: These findings indicate that TRIM45 plays an inhibitory role in the tumorigenesis of lung cancer. High-level expression of TRIM45 in lung cancer tissue may promote cell apoptosis by activating p38 signal and inhibit proliferation by down-regulating p-ERK, which provides a new clue for understanding the tumorigenesis of lung cancer.

5.
Curr Mol Med ; 20(4): 318-324, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31749426

RESUMO

BACKGROUND: Our previous studies have shown that Pygo (Pygopus) in Drosophila plays a critical role in adult heart function that is likely conserved in mammals. However, its role in the differentiation of human umbilical cord mesenchymal stem cells (hUC-MSCs) into cardiomyocytes remains unknown. OBJECTIVE: To investigate the role of pygo2 in the differentiation of hUC-MSCs into cardiomyocytes. METHODS: Third passage hUC-MSCs were divided into two groups: a p+ group infected with the GV492-pygo2 virus and a p- group infected with the GV492 virus. After infection and 3 or 21 days of incubation, Quantitative real-time PCR (qRT-PCR) was performed to detect pluripotency markers, including OCT-4 and SOX2. Nkx2.5, Gata-4 and cTnT were detected by immunofluorescence at 7, 14 and 21 days post-infection, respectively. Expression of cardiac-related genes-including Nkx2.5, Gata-4, TNNT2, MEF2c, ISL-1, FOXH1, KDR, αMHC and α-Actin-were analyzed by qRT-PCR following transfection with the virus at one, two and three weeks. RESULTS: After three days of incubation, there were no significant changes in the expression of the pluripotency stem cell markers OCT-4 and SOX2 in the p+ group hUC-MSCs relative to controls (OCT-4: 1.03 ± 0.096 VS 1, P > 0.05, SOX2: 1.071 ± 0.189 VS 1, P > 0.05); however, after 21 days, significant decreases were observed (OCT-4: 0.164 ± 0.098 VS 1, P < 0.01, SOX2: 0.209 ± 0.109 VS 1, P < 0.001). Seven days following incubation, expression of mesoderm specialisation markers, such as Nkx2.5, Gata-4, MEF2c and KDR, were increased; at 14 days following incubation, expression of cardiac genes, such as Nkx2.5, Gata-4, TNNT2, MEF2c, ISL-1, FOXH1, KDR, αMHC and α-Actin, were significantly upregulated in the p+ group relative to the p- group (P < 0.05). Taken together, these findings suggest that overexpression of pygo2 results in more hUCMSCs gradually differentiating into cardiomyocyte-like cells. CONCLUSION: We are the first to show that overexpression of pygo2 significantly enhances the expression of cardiac-genic genes, including Nkx2.5 and Gata-4, and promotes the differentiation of hUC-MSCs into cardiomyocyte-like cells.

6.
Artigo em Inglês | MEDLINE | ID: mdl-31717940

RESUMO

Using the Yellow-River-Source National Park (YRSNP) as a study site, an unmanned aerial vehicle (UAV) remote sensing and line transect method was used to investigate the number of wild herbivorous animals and livestock, including the kiang (Equus kiang) and Tibetan gazelle (Procapra picticaudata). A downscaling algorithm was used to generate the forage yield data in YRSNP based on a 30-m spatial resolution. On this basis, we estimated the forage-livestock balance, which included both wild animals and livestock, and analyzed the effects of functional zone planning in national parks on the forage-livestock balance in YRSNP. The results showed that the estimates of large herbivore population numbers in YRSNP based on population density in the aerial sample strips, which were compared and validated with official statistics and warm season survey results, indicated that the numbers of kiangs and Tibetan gazelles in the 2017 cold season were 12,900 and 12,100, respectively. The numbers of domestic yaks, Tibetan sheep, and horses were 53,400, 76,800, and 800, respectively, and the total number of sheep units was 353,200. The ratio of large wild herbivores and livestock sheep units was 1:5. Large wild herbivores have different preferences for functional zones, preferring ecological restoration areas consisting mainly of sparse grassland. The grazing pressure indices of the core reserve areas and ecological restoration areas were 0.168 and 0.276, respectively, indicating that these two regions still have high grazing potential. However, the grazing pressure index of the traditional utilization areas was 1.754, indicating that these grasslands are severely overloaded. After the planning and implementation of functional zones, the grazing pressure index of YRSNP was 1.967. Under this measure, the number of livestock was not reduced and the grazing pressure nearly doubled, indicating that forage-livestock conflict has become more severe in YRSNP.


Assuntos
Herbivoria , Parques Recreativos , Animais , Bovinos , Censos , China , Pradaria , Cavalos , Gado , Densidade Demográfica , Tecnologia de Sensoriamento Remoto , Rios , Estações do Ano , Ovinos
7.
Ecol Evol ; 9(17): 9634-9643, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31534681

RESUMO

Aims: We aim to understand bird richness and variation in species composition (beta diversity) along a 630 km riparian landscape in the Altai Mountains of China and to test whether vegetation cover is the main explanation of species diversity. Methods: We selected nine regions along a gradient of natural vegetation change. Bird surveys and environmental measurements were conducted at 10 points in each of the nine regions. We collected environmental land cover variables such as wood cover (area proportion of trees and shrubs with saplings in habitats; here trees are woody plant with a single trunk and higher than 3 m, shrubs and saplings are distinguished from trees by their multiple trunks and shorter height) and tree cover, and two climate factors which were Annual Mean Temperature (AMT) and Annual Precipitation (AP). We used Liner Regression Models to explore the correlation between bird species richness and environmental variables. We used Sørensen's dissimilarity index to measure birds' beta diversity, and quantified the contribution of environmental variables to this pattern using a Canonical Correspondence Analysis (CCA). Results: Wood cover was the strongest predictor of overall, insectivore, and omnivore bird richness. Regions with wood cover contained more bird species. Beta diversity was overall high in the studied regions, and turnover components occupied a major part of beta diversity. Wood cover and AP were significant predictors of bird species composition explaining 33.24% of bird beta diversity together. Conclusions: Wood vegetation including trees, shrubs, and saplings, rather than only trees, contains high bird richness. High beta diversity suggests that expansion of the existing nature reserves is needed in the riparian landscapes to capture the variation in bird species composition. Thus all wood cover in the overall riparian landscapes of Altai Mountains should be protected from farming and grazing to improve bird conservation outcomes. OPEN RESEARCH BADGES: This article has earned an Open Data Badge for making publicly available the digitally-shareable data necessary to reproduce the reported results. The data is available at Raw bird data in this study: osf.io/78qcw; Raw environment data: osf.io/qr5cw.

8.
BMC Public Health ; 19(1): 1052, 2019 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-31382935

RESUMO

It has been highlighted that the original article [1] contained some errors in the Result section of the Abstract. The incorrect and correct statement is shown in the Correction article.

9.
Genet Test Mol Biomarkers ; 23(9): 601-609, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31386585

RESUMO

Background: Tetralogy of Fallot (TOF) accounts for ∼10% of congenital heart disease cases. The blood vessel epicardial substance (BVES) gene has been reported to play a role in the function of adult hearts. However, whether allelic variants in BVES contribute to the risk of TOF and its possible mechanism remains unknown. Methods: The open reading frame of the BVES gene was sequenced using samples from 146 TOF patients and 100 unrelated healthy controls. qRT-PCR and western blot assays were used to confirm the expression of mutated BVES variants in the TOF samples. The online software Polyphen2 and SIFT were used to predict the deleterious effects of the observed allelic variants. The effects of these allelic variants on the transcriptional activities of genes were examined using dual-fluorescence reporter assays. Results: We genotyped four single nucleotide polymorphisms (SNPs) in the BVES gene from each of the 146 TOF patients. Among them, the minor allelic frequencies of c.385C>T (p.R129W) were 0.035% in TOF, but ∼0.025% in 100 controls and the Chinese Millionome Database. This allelic variant was predicted to be a potentially harmful alteration by the Polyphen2 and SIFT softwares. qRT-PCR and western blot analyses indicated that the expression of BVES in the six right ventricular outflow tract samples with the c.385C>T allelic variant was significantly downregulated. A dual-fluorescence reporter system showed that the c.385C>T allelic variant significantly decreased the transcriptional activity of the BVES gene and also decreased transcription from the GATA4 and NKX2.5 promoters. Conclusions: c.385C>T (p.R129W) is a functional SNP of the BVES gene that reduces the transcriptional activity of BVES in vitro and in vivo in TOF tissues. This subsequently affects the transcriptional activities of GATA4 and NKX2.5 related to TOF. These findings suggest that c.385C>T may be associated with the risk of TOF in the Han Chinese population.


Assuntos
Moléculas de Adesão Celular/genética , Proteínas Musculares/genética , Tetralogia de Fallot/genética , Alelos , Grupo com Ancestrais do Continente Asiático/genética , Moléculas de Adesão Celular/metabolismo , China/etnologia , Fator de Transcrição GATA4/metabolismo , Genótipo , Proteína Homeobox Nkx-2.5/metabolismo , Humanos , Proteínas Musculares/metabolismo , Polimorfismo de Nucleotídeo Único , Fatores de Risco , Análise de Sequência de DNA/métodos
10.
Nano Lett ; 19(10): 7043-7049, 2019 10 09.
Artigo em Inglês | MEDLINE | ID: mdl-31468976

RESUMO

Circular-polarization-resolved magneto-infrared studies of multilayer epitaxial graphene (MEG) are performed using tunable quantum cascade lasers in high magnetic fields up to 17.5 T. Landau level (LL) transitions in the monolayer and bilayer graphene inclusions of MEG are resolved, and considerable electron-hole asymmetry is observed in the extracted electronic band structure. For monolayer graphene, a four-fold splitting of the n = 0 to n = 1 LL transition is evidenced and attributed to the lifting of the valley and spin degeneracy of the zeroth LL and the broken electron-hole symmetry. The magnetic field dependence of the splitting further reveals its possible mechanisms. The best fit to experimental data yields effective g-factors, gVS* = 6.7 and gZS* = 4.8, for the valley and Zeeman splittings, respectively.

11.
Conserv Biol ; 33(5): 991-992, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31329341
12.
Dalton Trans ; 48(24): 8920-8930, 2019 Jun 18.
Artigo em Inglês | MEDLINE | ID: mdl-31210236

RESUMO

Platinum is the most active and commonly used electrocatalyst for hydrogen evolution reaction (HER). However, its expensive price and scarce supply limit its world-wide use for mass production of H2 by water electrolysis. Promising candidates for high-performance hydrogen evolution reaction with exceptionally low Pt loading are urgently required to obtain clean and sustainable chemical fuels. Herein, yolk-shell Pt-CoP polyhedra were prepared to realize low Pt loading on low-cost CoP polyhedral substrates via low-temperature calcination and phosphorization of ZIF-67 combined with a subsequent microwave-assisted platinum reduction process. Owing to the unique yolk-shell structure, large surface area, numerous active sites, trace Pt modification and improved conductivity, the resultant catalyst exhibits highly efficient electrocatalytic activities for the HER with the low overpotentials of only 48 mV and 88 mV at 10 mA cm-2 and excellent stability in the 1 M KOH and 1 M PBS solution, superior to most of the newly reported noble metal-based or transition metal-based catalysts, even the state-of-art Pt/C catalysts.

13.
BMC Public Health ; 19(1): 723, 2019 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-31182075

RESUMO

BACKGROUND: Indoor environmental quality significantly influences the occurrence of asthma attack. Zunyi District has abundant coal reserves and is regarded as one of the cities that are most severely polluted by high levels of particulate matter in China. This study aimed to examine the correlation of indoor exposure with adult respiratory health, as well as the differences in effect between winter and summer. METHODS: A cross-sectional epidemiological study was conducted among 1207 adult residents in Zunyi, Guizhou Province of Southwest China in winter and summer. Data on health variables related to asthma and home environmental factors were collected using a modified European Community Respiratory Health Survey II questionnaire. The following data were obtained: samples of particulate matter 2.5 (PM2.5) inside and outside the households under study (n = 20); lung function status, including peak expiratory flow rate, forced vital capacity (FVC), forced expiratory volume in 1 s (FEV1), and FEV1/FVC ratio. RESULT: The odds ratio (OR) for asthma-like symptoms and asthma in adults using coal stove for cooking or warming, relative to non-users, was 1.73 (95% CI, 1.11-2.69) in winter vs. 1.30 (95% CI, 0.79-2.14) in summer. Adult residents with exposure to cooking oil fumes were at a considerably higher risk of asthma-like symptoms and asthma [OR = 2.65 (95% CI, 1.25 to 5.61) in winter vs. OR = 7.93 (95% CI, 2.54 to 24.75] in summer] than those without such exposure. The prevalence of asthma-like symptoms and asthma was significantly higher in adults with high kitchen risk scores or high sleeping-area risk scores than in those with low scores in both seasons (p < 0.05). The relative kitchen and sleeping area PM2.5 concentrations were higher in winter than in summer (p < 0.05). Lung function was negatively associated with indoor kitchen and sleeping area relative PM2.5 concentration in winter rather than summer (p < 0.001). The effect of exposure to indoor risk factors on lung function among the residents was greater in winter than in summer (p < 0.001). CONCLUSION: Exposure to indoor risk factors, such as aerocontaminants from coal combustion, causes asthma symptoms and reduces pulmonary function. The effect of indoor risk factors on respiratory health among adults with such exposure was greater in winter than in summer.


Assuntos
Poluentes Atmosféricos/análise , Poluição do Ar em Ambientes Fechados/análise , Asma/epidemiologia , Exposição Ambiental/efeitos adversos , Material Particulado/análise , Adulto , Asma/etiologia , China/epidemiologia , Cidades , Carvão Mineral , Culinária , Estudos Transversais , Feminino , Humanos , Pulmão/fisiopatologia , Masculino , Pessoa de Meia-Idade , Testes de Função Respiratória , Fatores de Risco , Estações do Ano
14.
Front Immunol ; 10: 1286, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31231392

RESUMO

Purpose: We aimed to retrospectively analyze the clinical features, laboratory and imaging results, and predictors of poor prognosis for patients with an initial diagnosis of autoimmune encephalitis (AE) at the Affiliated Hospital of Zunyi Medical University. Methods: Fifty patients with an initial diagnosis of AE who were admitted to our hospital from May 2014 to May 2018 were enrolled retrospectively. Clinical characteristics and experimental test data, including the neutrophil-to-lymphocyte ratio (NLR), were collected from medical records within 24 h of admission. Independent prognostic factors were determined by multivariate logistic regression analysis. A good or poor prognosis for patients was defined based on the modified Rankin Scale (mRS). The correlation between the immunotherapy latency and prognostic mRS score was determined using the Spearman rank correlation test. Results: Univariate analysis indicated that increased NLR (P = 0.001), decreased lymphocyte counts (P = 0.001), low serum albumin (P = 0.017), consciousness disorders (P = 0.001), epileptic seizures (P = 0.007), extrapyramidal symptoms (P = 0.042), abnormal electroencephalogram (EEG) findings (P = 0.001), abnormal brain magnetic resonance imaging (MRI) findings (P = 0.003), and pulmonary infection complications (P = 0.000) were associated with the poor prognosis of AE. Multivariate logistic regression analysis showed that NLR (odds ratio [OR] 2.169, 95% confidence interval [CI] 1.029-4.570; P < 0.05) was an independent risk factor for predicting the poor prognosis of AE. NLR > 4.45 was suggested as the cut-off threshold for predicting the adverse outcomes of AE. In addition, we revealed that there was a positive correlation between immunotherapy latency and mRS score (rs = 0.535, P < 0.05). Conclusions: NLR may have predictive value for the poor outcomes of AE. Early initiation of immunotherapy is associated with a good prognosis.

15.
Proc Natl Acad Sci U S A ; 116(28): 14181-14190, 2019 07 09.
Artigo em Inglês | MEDLINE | ID: mdl-31068469

RESUMO

Extracellular matrix (ECM) deposition is a hallmark of many diseases, including cancer and fibroses. To exploit the ECM as an imaging and therapeutic target, we developed alpaca-derived libraries of "nanobodies" against disease-associated ECM proteins. We describe here one such nanobody, NJB2, specific for an alternatively spliced domain of fibronectin expressed in disease ECM and neovasculature. We showed by noninvasive in vivo immuno-PET/CT imaging that NJB2 detects primary tumors and metastatic sites with excellent specificity in multiple models of breast cancer, including human and mouse triple-negative breast cancer, and in melanoma. We also imaged mice with pancreatic ductal adenocarcinoma (PDAC) in which NJB2 was able to detect not only PDAC tumors but also early pancreatic lesions called pancreatic intraepithelial neoplasias, which are challenging to detect by any current imaging modalities, with excellent clarity and signal-to-noise ratios that outperformed conventional 2-fluorodeoxyglucose PET/CT imaging. NJB2 also detected pulmonary fibrosis in a bleomycin-induced fibrosis model. We propose NJB2 and similar anti-ECM nanobodies as powerful tools for noninvasive detection of tumors, metastatic lesions, and fibroses. Furthermore, the selective recognition of disease tissues makes NJB2 a promising candidate for nanobody-based therapeutic applications.


Assuntos
Carcinogênese/genética , Carcinoma Ductal Pancreático/diagnóstico por imagem , Matriz Extracelular/efeitos dos fármacos , Neoplasias Pancreáticas/diagnóstico por imagem , Animais , Neoplasias da Mama/diagnóstico por imagem , Neoplasias da Mama/patologia , Carcinoma Ductal Pancreático/patologia , Linhagem Celular Tumoral , Matriz Extracelular/metabolismo , Matriz Extracelular/patologia , Feminino , Fibrose/patologia , Humanos , Masculino , Camundongos , Neoplasias Pancreáticas/patologia , Tomografia Computadorizada com Tomografia por Emissão de Pósitrons , Neoplasias da Próstata/diagnóstico por imagem , Neoplasias da Próstata/patologia , Compostos Radiofarmacêuticos/farmacologia , Anticorpos de Domínio Único/química , Anticorpos de Domínio Único/farmacologia
16.
Sci Rep ; 9(1): 4895, 2019 03 20.
Artigo em Inglês | MEDLINE | ID: mdl-30894581

RESUMO

Musk deer Moschus spp. are endemic to the high mountain forests of central Asia. The taxonomic status of musk deer in the central and western Himalayas is poorly understood. We investigated the phylogenetic relationship of musk deer from the central and western Himalayas based on mitochondrial genomic data of Cytochrome b (380 bps) and D-loop (1000 bps). Our results distinguished two divergent lineages using higher bootstrap support (bs) values from the Maximum likelihood and Bayesian posterior probabilities (bpp). Both the Manang and Kaski lineages from central Nepal are confirmed as Himalayan musk deer Moschus leucogaster and represent a species complex widespread throughout the central and eastern Himalayan region. The musk deer Mustang lineage was confirmed as Kashmir musk deer Moschus cupreus and has wide distribution in the western Himalayas (from central Nepal to Afghanistan). Our analysis validates that Kashmir musk deer is a genetically distinct species and it clarifies that Himalayan musk deer and Kashmir musk deer are confirmed instead of Alpine musk deer Moschus chrysogaster which has been previously described from the southern parts of Himalayas of Nepal, India and Pakistan.

17.
Cancer Lett ; 449: 87-98, 2019 05 01.
Artigo em Inglês | MEDLINE | ID: mdl-30768955

RESUMO

Pancreatic ductal adenocarcinoma (PDAC) is an extremely aggressive malignancy with very limited treatment options. Antibody drug conjugates (ADCs) are promising cytotoxic agents capable of highly selective delivery. Aspartate-ß-hydroxylase (ASPH) is a type II transmembrane protein highly expressed in PDACs (97.1%) but not normal pancreas. We investigated anti-tumor effects of an ADC guided by a human monoclonal antibody (SNS-622) against ASPH in human PDAC cell lines and derived subcutaneous (s.c.) xenograft as well as a patient-derived xenograft (PDX) murine model with spontaneous pulmonary metastasis. The cytotoxic effects exhibited by several candidate payloads linked to SNS-622 antibody targeting ASPH+ PDACs were analyzed. After i.v. administration of SNS-622-emtansine (DM1) ADC, the primary PDAC tumor growth and progression (number and size of pulmonary metastases) were determined. The PDAC cell lines, s.c. and PDX tumors treated with ADC were tested for cell proliferation, cytotoxicity and apoptosis by MTS and immunohistochemistry (IHC) assays. SNS-622-DM1 construct has demonstrated optimal anti-tumor effects in vitro. In the PDX model of human PDAC, SNS-622-DM1 ADC exerted substantially inhibitory effects on tumor growth and pulmonary metastasis through attenuating proliferation and promoting apoptosis.


Assuntos
Proteínas de Ligação ao Cálcio/antagonistas & inibidores , Carcinoma Ductal Pancreático/tratamento farmacológico , Imunoconjugados/administração & dosagem , Neoplasias Pulmonares/tratamento farmacológico , Neoplasias Pulmonares/secundário , Proteínas de Membrana/antagonistas & inibidores , Oxigenases de Função Mista/antagonistas & inibidores , Proteínas Musculares/antagonistas & inibidores , Neoplasias Pancreáticas/tratamento farmacológico , Administração Intravenosa , Animais , Carcinoma Ductal Pancreático/enzimologia , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Humanos , Imunoconjugados/farmacologia , Neoplasias Pulmonares/enzimologia , Camundongos , Neoplasias Pancreáticas/enzimologia , Regulação para Cima/efeitos dos fármacos , Ensaios Antitumorais Modelo de Xenoenxerto
18.
Sci Total Environ ; 665: 23-32, 2019 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-30772553

RESUMO

The energy-saving and emission reduction (ESER) strategy is a crucial measure for promoting the sustainability of manufacturing industry in green transition. Analyzing current practices and limitations of the ESER in the manufacturing industry, this paper proposes a new concept entitled lean energy-saving and emission-reduction (LESER) and an approach to effectively improve the energy efficiency and reduce waste emissions. This paper illustrates the definition of the LESER and establishes an implementation framework for LESER to improve the manufacturing process. To quantify and evaluate performance of LESER, the state space model of the carbon footprint for energy consumption and waste discharge is established. A method for implementing the LESER strategy is constructed in the following steps: (i) clarification of the current situation; (ii) analysis of the root cause; (iii) improvement; (iv) evaluation of the carbon emissions; (v) sustaining and standardizing. Finally, the LESER strategy is applied to the Zcrubber Group Co. Ltd., which is characterized with high pollution, high energy consumption, and high emission in green transition. Results demonstrate practicability of the proposed strategy to offer an effective measure for promoting sustainability of manufacturing industry.

19.
Toxicology ; 414: 27-34, 2019 02 15.
Artigo em Inglês | MEDLINE | ID: mdl-30629985

RESUMO

OBJECTIVE: We investigated the impact of subchronic low-dose exposure to nonylphenol (NP) on depression-like behaviors and synaptic morphological plasticity in the context of a high-sucrose/high-fat diet in rats. METHODS: Male Sprague Dawley (SD) rats were randomly divided into 8 groups (n = 10 per group), as follows: rats fed a normal-diet (ND), as the control (C-ND); rats fed a normal diet and gavaged with NP at a dose of 0.02 mg/kg/day (NP-L-ND), 0.2 mg/kg/day (NP-M-ND) or 2 mg/kg/day (NP-H-ND); rats fed a high-sucrose/high-fat diet (HSHFD), as the HSHFD control (C-HSHFD); rats fed a HSHFD and gavaged with NP at a dose of 0.02 mg/kg/day (NP-L-HSHFD), 0.2 mg/kg/day (NP-M-HSHFD) or 2 mg/kg/day (NP-H-HSHFD). Elevated plus maze was used to evaluate anxiety behavior. Open field test was used to evaluate locomotor activity. Cyclooxygenase-2 expression in hippocampal tissue was measured by immunohistochemistry. The ultrastructure of hippocampal mitochondria and the synaptic plasticity were observed by transmission electron microscopy. RESULTS: Significant interactions between HSHFD and NP-H were observed, reflected by the time spent exploring the open arms, time spent in the center area, distance traveled in the center area and total distance traveled (p < 0.05). Exposure to NP-H-HSHFD resulted in swelling of the mitochondria, associated with an increased number of disordered and partially disrupted cristae compared with the control group. Synaptic interface curvatures and postsynaptic density thickness decreased as the NP dose increased among the treatment groups. Co-exposure to HSHFD and NP showed an increase in synaptic cleft width compared with the HSHFD-only and NP-only exposure groups (p < 0.05). COX-2 expression and integral optical density value increased as the NP dose increased among the NP treatment groups (p < 0.05). CONCLUSION: Subchronic low-dose exposure to NP might induce alterations in depression-like behaviors, synaptic morphological plasticity and COX-2 expression in the hippocampus. Co-exposure to NP and HSHFD had significantly more dissimilarities.


Assuntos
Comportamento Animal/efeitos dos fármacos , Depressão/induzido quimicamente , Dieta Hiperlipídica , Sacarose na Dieta , Disruptores Endócrinos/toxicidade , Hipocampo/efeitos dos fármacos , Plasticidade Neuronal/efeitos dos fármacos , Fenóis/toxicidade , Animais , Ciclo-Oxigenase 2/metabolismo , Depressão/patologia , Depressão/fisiopatologia , Depressão/psicologia , Modelos Animais de Doenças , Hipocampo/metabolismo , Hipocampo/fisiopatologia , Hipocampo/ultraestrutura , Locomoção/efeitos dos fármacos , Masculino , Aprendizagem em Labirinto/efeitos dos fármacos , Ratos Sprague-Dawley , Medição de Risco
20.
Ecol Evol ; 9(1): 4-18, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30680091

RESUMO

Himalayan musk deer (Moschus leucogaster; hereafter musk deer) are endangered as a result of poaching and habitat loss. The species is nocturnal, crepuscular, and elusive, making direct observation of habitat use and behavior difficult. However, musk deer establish and repeatedly use the same latrines for defecation. To quantify musk deer habitat correlates, we used observational spatial data based on presence-absence of musk deer latrines, as well as a range of fine spatial-scale ecological covariates. To determine presence-absence of musk deer, we exhaustively searched randomly selected forest trails using a 20-m belt transect in different study sites within the Neshyang Valley in the Annapurna Conservation Area. In a subsequent way, study sites were classified as habitat or nonhabitat for musk deer. A total of 252 plots, 20 × 20 m, were systematically established every 100 m along 51 transects (each ~0.5 km long) laid out at different elevations to record a range of ecological habitat variables. We used mixed-effect models and principal component analysis to characterize relationships between deer presence-absence data and habitat variables. We confirmed musk deer use latrines in forests located at higher elevations (3,200-4,200 m) throughout multiple seasons and years. Himalayan birch (Betula utilis) dominated forest, mixed Himalayan fir (Abies spectabilis), and birch forest were preferred over pure Himalayan fir and blue pine (Pinus wallichiana) forest. Greater crown cover and shrub diversity were associated with the presence of musk deer whereas tree height, diameter, and diversity were weakly correlated. Topographical attributes including aspect, elevation, distance to water source, and slope were also discriminated by musk deer. Over- and understory forest management can be used to protect forests likely to have musk deer as predicted by the models to ensure long-term conservation of this rare deer.

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