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1.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 29(5): 1504-1509, 2021 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-34627431

RESUMO

OBJECTIVE: To investigate the effect of arsenic disulfide (AS2S2) combined with itraconazole on the proliferation, apoptosis and hedgehog pathway of diffuse large B-cell lymphoma (DLBCL) cells. METHODS: The human DLBCL cell OCI-LY3 was treated with different concentrations of AS2S2 and itraconazole. Cell proliferation inhibition was detected by CCK-8, cell apoptosis rate was determined by flow cytometry. The expression levels of BCL-2, BAX, SMO and GLi1 were detected by Western blot. RESULTS: The DLBCL cell viability was decreased significantly at 24, 48 or 72 h as cultured with itraconazole. Along with the increasing of itraconazole concentration, the DLBCL cell viability was significantly reduced as compared with that in control group, and the results showed statistically significant(r=-0.690,r=-0.639, r=-0.833, r=-0.808, r=-0.578). The inhibitory and apoptosis rates of the cells were significantly increased as compared with those of the single drug-treated group after treated by the combination of itraconazole and AS2S2(P<0.05). The protein levels of SMO and Glil were significantly down-regulated after treated by arsenic disulfide and itraconazole alone(P<0.01). The protein expression levels of SMO and Glil was down-regulated in the combined-treatment group(P<0.01). CONCLUSION: Itraconazole can inhibit proliferation of DLBCL cells in a concentration-and time-dependent manner. In addition, the combination of AS2S2 and itraconazole show a synergistic effects, which may be related with the down-regulated protein expression of SMO and Glil of Hedgehog signaling pathway.


Assuntos
Proteínas Hedgehog , Linfoma Difuso de Grandes Células B , Apoptose , Arsenicais , Humanos , Itraconazol/farmacologia , Linfoma Difuso de Grandes Células B/tratamento farmacológico , Sulfetos
2.
Front Public Health ; 9: 708496, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34660510

RESUMO

Objective: This study aimed to examine the effectiveness of containment strategies and mitigation strategies to provide a reference for controlling the ongoing global spread of the pandemic. Methods: We extracted publicly available data from various official websites between January 1 and December 31, 2020, summarized the strategies implemented in China, South Korea, Singapore, the United States, the United Kingdom, and France, and assessed the effectiveness of the prevention and control measures adopted by these countries with the daily new cases and mortality rate per 100,000 population. Results: China, South Korea, and Singapore adopted containment strategies, which maintained a proactive approach by identifying and managing cases, tracking and isolating close contacts. China and Singapore had a similar epidemic curve and the new daily cases. As of December 31, 2020, the new daily cases of China and Singapore were below 100 with the mortality rates per 100,000 population of 0.3 and 0.5, respectively. But the new daily case of South Korea was as high as 1,029, with a mortality rate per 100,000 population of 1.8. In contrast, the United States, the United Kingdom, and France responded with mitigation strategies that focus on treating severe cases and those with underlying conditions. They had similar epidemic curves and mortality rates per 100,000 population. The United States had up to 234,133 new confirmed cases per day, and the mortality rate per 100,000 population was 107, while the United Kingdom had 56,029 new confirmed cases per day and the mortality rate per 100,000 population was 108, and France had 20,042 new cases per day, with a mortality rate per 100,000 population of 99. Conclusions: China, Korea, and Singapore, which implemented strict containment measures, had significant outbreak control. Meanwhile, the successful practices in China, Singapore, and South Korea show that the containment strategies were practices that work especially at the individual level identifying and managing the infected patients and their close contacts. In the United States, the United Kingdom, and France, which implemented the mitigation policies, the effect of epidemic prevention and control was not significant that the epidemic continued or even increased epidemic relatively quickly.


Assuntos
COVID-19 , Pandemias , China/epidemiologia , Humanos , Pandemias/prevenção & controle , República da Coreia/epidemiologia , SARS-CoV-2 , Estados Unidos/epidemiologia
3.
Front Microbiol ; 12: 736484, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34621258

RESUMO

Rhipicephalus microplus, a vector that can transmit many pathogens to humans and domestic animals, is widely distributed in Yunnan province, China. However, few reports on the prevalence of tick-borne pathogens (TBPs) in Rh. microplus in Yunnan are available. The aim of this study was to detect TBPs in Rh. microplus in Yunnan and to analyze the phylogenetic characterization of TBPs detected in these ticks. The adult Rh. microplus (n = 516) feeding on cattle were collected. The pooled DNA samples of these ticks were evaluated using metagenomic next-generation sequencing (mNGS) and then TBPs in individual ticks were identified using genus- or group-specific nested polymerase chain reaction (PCR) combined with DNA sequencing assay. As a result, Candidatus Rickettsia jingxinensis (24.61%, 127/516), Anaplasma marginale (13.18%, 68/516), Coxiella burnetii (3.10%, 16/516), and Coxiella-like endosymbiont (CLE) (8.33%, 43/516) were detected. The dual coinfection with Ca. R. jingxinensis and A. marginale and the triple coinfection with Ca. R. jingxinensis, A. marginale, and CLE were most frequent and detected in 3.68% (19/516) and 3.10% (16/516) of these ticks, respectively. The results provide insight into the diversity of TBPs and their coinfections in Rh. microplus in Yunnan province of China, reporting for the first time that C. burnetii had been found in Rh. microplus in China. Multilocus variable number tandem repeat analysis with 6 loci (MLVA-6) discriminated the C. burnetii detected in Rh. microplus in Yunnan into MLVA genotype 1, which is closely related to previously described genotypes found primarily in tick and human samples from different regions of the globe, indicating a potential public health threat posed by C. burnetii in Rh. microplus in Yunnan.

4.
Artigo em Inglês | MEDLINE | ID: mdl-34503423

RESUMO

BACKGROUND: Extranodal natural killer/T cell lymphoma (ENKTL) is an aggressive malignant non-Hodgkin's lymphoma (NHL) with a poor prognosis. Therefore, novel therapeutic biomarkers and agents must be identified for the same. KAT5 inhibitor, NU 9056, is a small molecule that can inhibit cellular proliferation; however, its role in ENKTL has not been studied. OBJECTIVE: The present study investigated the effect of NU 9056 in ENKTL cells and explored the possible molecular mechanism for its antitumour effect. METHODS: The role of NU 9056 in ENKTL cells was investigated through the Cell Counting Kit-8 assay, flow cytometry, Western blot, and real-time quantitative polymerase chain reaction assay. RESULTS: NU 9056 inhibited ENKTL cell proliferation and induced G2/M phase arrest. NU 9056 also induced apoptosis by upregulating DR4, DR5, and caspase 8 expressions. Additionally, NU 9056 increased the expression of Bax, Bid, and cytochrome C and decreased the expression of Bcl-2, Mcl-1, and XIAP. Furthermore, NU 9056 activated endoplasmic reticulum (ER) stress and inhibited the JAK2/STAT3 signalling pathway. The p38 mitogen-activated protein kinase (MAPK) signalling pathway was also activated by NU 9056, and the ERK signalling pathway was suppressed in natural killer/T cell lymphoma cells. CONCLUSION: NU 9056 inhibited cell proliferation, arrested cell cycle in the G2/M phase, and induced apoptosis through the stimulation of ER stress, thus inhibiting the JAK2/STAT3 signalling pathway and regulating MAPK pathways in ENKTL cells.

5.
Risk Manag Healthc Policy ; 14: 3323-3332, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34408516

RESUMO

Objective: This study aimed to make a comparative analysis of the public health containment measures between China and India, explore the causes of the serious COVID-19 epidemic in India, and eventually to improve global infectious disease control. Methods: We extracted publicly available data from official websites, summarized the containment measures implemented in China and India, and assessed their effectiveness. Results: China has responded to the COVID-19 outbreak with strict public health containment measures, including lockdown of Wuhan city, active case tracing, and large-scale testing, ultimately preventing a large increase in daily new cases and maintaining a low mortality rate per million population (as of May 5, 2021, daily new cases were 11 and mortality rate per million population was 3.37). India, although imposing a national lockdown to control the pandemic, has not implemented strict testing, tracking, and quarantine measures due to the overburdened healthcare system. Combined with massive lockdown, it has accelerated human mobility and exacerbated the epidemic, resulting in a rapid increase in daily new cases and a high mortality rate per million population (as of May 5, 2021, daily new cases were 412,431 and mortality rate per million population was 166.79). Conclusion: China and India implemented public health containment measures to contain the spread of the COVID-19 pandemic based on their national situations. Meanwhile, daily new cases and mortality of COVID-19 also were affected by environmental and socioeconomic. Countries make a comprehensive strategy not only in terms of the biological, pharmaceutical, health, and sanitation sectors but also based on sustainability science and environmental science.

6.
PLoS Negl Trop Dis ; 15(7): e0009520, 2021 07.
Artigo em Inglês | MEDLINE | ID: mdl-34264939

RESUMO

Q fever is a worldwide zoonosis caused by Coxiella burnetii (Cb). From January 2018 to November 2019, plasma samples from 2,382 patients with acute fever of unknown cause at a hospital in Zhuhai city of China were tested using metagenomic next-generation sequencing (mNGS). Of those tested, 138 patients (5.8%) were diagnosed with Q fever based on the presence of Cb genomic DNA detected by mNGS. Among these, 78 cases (56.5%) presented from Nov 2018 to Mar 2019, suggesting an outbreak of Q fever. 55 cases with detailed clinical information that occurred during the outbreak period were used for further analysis. The vast majority of plasma samples from those Cb-mNGS-positive patients were positive in a Cb-specific quantitative polymerase chain reaction (n = 38) and/or indirect immunofluorescence assay (n = 26). Mobile phone tracing data was used to define the area of infection during the outbreak. This suggested the probable infection source was Cb-infected goats and cattle at the only official authorized slaughterhouse in Zhuhai city. Phylogenic analysis based on genomic sequences indicated Cb strains identified in the patients, goat and cattle were formed a single branch, most closely related to the genomic group of Cb dominated by strains isolated from goats. Our study demonstrates Q fever was epidemic in 2018-2019 in Zhuhai city, and this is the first confirmed epidemic of Q fever in a contemporary city in China.


Assuntos
Coxiella burnetii/isolamento & purificação , Febre Q/microbiologia , Adulto , Animais , Bovinos , Doenças dos Bovinos/epidemiologia , Doenças dos Bovinos/microbiologia , Doenças dos Bovinos/transmissão , China/epidemiologia , Coxiella burnetii/classificação , Coxiella burnetii/genética , Surtos de Doenças , Feminino , Doenças das Cabras/epidemiologia , Doenças das Cabras/microbiologia , Doenças das Cabras/transmissão , Cabras , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Masculino , Metagenômica , Pessoa de Meia-Idade , Filogenia , Febre Q/diagnóstico , Febre Q/epidemiologia , Febre Q/transmissão , Adulto Jovem , Zoonoses/diagnóstico , Zoonoses/epidemiologia , Zoonoses/microbiologia , Zoonoses/transmissão
7.
Zhongguo Zhen Jiu ; 41(7): 767-73, 2021 Jul 12.
Artigo em Chinês | MEDLINE | ID: mdl-34259410

RESUMO

OBJECTIVE: To observe the efficacy of Jiannao Anshen acupuncture (acupuncture for brain-invigorating and mind-calming) on insomnia, and the dynamic changes of functional magnetic resonance imaging (fMRI) before and after acupuncture, to provide neuroimaging basis of acupuncture for insomnia. METHODS: A total of 58 participants were included. Of them, 29 patients with insomnia were included into an observation group, and 29 healthy participants were included into a control group. The patients in the observation group were treated with Jiannao Anshen acupuncture at Baihui (GV 20), bilateral Sishencong (EX-HN 1), Fengfu (GV 16), Fengchi (GB 20), Qiangjian (GV 18), occipital sideline, Anmian (Extra), Yiming (EX-HN 14) and Dazhui (GV 14); treatment was given once a day, 10 days as a course of treatment, and a total of 2 courses of treatment was given. The resting-state fMRI data was collected in the observation group before and after treatment. The participants in the control group received no treatment, and the resting-state fMRI data was collected only once. The resting-state fMRI data in the observation group before treatment and in the control group, also the observation group before and after treatment were compared. The Pittsburgh sleep quality index (PSQI), insomnia severity index (ISI), daytime insomnia symptom scale (DISS), hyperarousal scale (HAS), Hamilton anxiety scale (HAMA), Hamilton depression scale (HAMD), digit symbol test, mini-mental state examination (MMSE), Montreal cognitive assessment (MoCA) and the A, B speed of trail making test (NCT) were observed in the observation group before and after treatment. The clinical efficacy of the observation group was evaluated. RESULTS: The total effective rate was 89.7% (26/29) in the observation group. In the observation group, the scores of PSQI, ISI and DISS, the A, B speed of NCT were all decreased after treatment (P<0.01, P<0.05). Before treatment, compared with the control group, functional connectivity in ventral dorsal prefrontal cortex, dorsal anterior cingulate cortex, inferior parietal lobe, dorsal lateral prefrontal cortex, dorsal anterior cingulate cortex, left ventral anterior cingulate gyrus and right ventral anterior cingulate gyrus was reduced in the observation group; compared before treatment, the functional connectivity in lingual gyrus, cerebellar area 6, frontal lobe, insular lobe and anterior cingulate gyrus, premotor gyrus and posterior cingulate gyrus was increased after treatment in the observation group. After treatment, the functional connection intensity of left ventral caudate nucleus and insular lobe was negatively correlated with HAMD score in the observation group (r =-0.55, P<0.05), and the functional connection intensity of left ventral caudate nucleus and anterior cingulate gyrus was negatively correlated with HAMA score in the observation group (r =-0.47, P<0.05). CONCLUSION: The Jiannao Anshen acupuncture could effectively improve the sleep quality in patients with insomnia, and acupuncture could enhance the connection of left dorsal, right ventral and left ventral caudate nucleus with brain area.


Assuntos
Terapia por Acupuntura , Distúrbios do Início e da Manutenção do Sono , Encéfalo/diagnóstico por imagem , Emoções , Humanos , Imageamento por Ressonância Magnética , Distúrbios do Início e da Manutenção do Sono/terapia
8.
Arch Virol ; 166(8): 2217-2224, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34091783

RESUMO

Swine influenza is an economically important respiratory disease in swine, but it also constantly poses a threat to human health. Therefore, developing rapid, sensitive, and efficient detection methods for swine influenza virus (SIV) is important. By aligning the haemagglutinin (HA) gene sequences of SIVs circulating in China over a 10-year period, an H1 primer-probe set targeting both Eurasian avian-like H1N1 (EA H1N1) and pandemic 2009 H1N1 ((H1N1)pdm09) lineages plus a H3 primer-probe set targeting the prevalent human-like H3N2 (HL H3N2) subtype were designed. Subsequently, a TaqMan-MGB-based duplex one-step real-time RT-PCR (RT-qPCR) assay was established and evaluated. The duplex RT-qPCR has a detection limit of 5 copies/µL of HA plasmid for EA H1N1, (H1N1)pdm09, and HL H3N2 subtype SIVs, and its overall detection sensitivity of 100% and specificity of 91.67% matches that of traditional virus isolation through chicken embryo inoculation using experimentally infected mouse lung samples. The method showed high repeatability both within run and between runs, and there was no cross-reactivity against several other porcine viruses that are commonly circulating in China. Furthermore, the duplex RT-qPCR method revealed a higher prevalence of subtype H1 than subtype H3 in 166 nasal swabs from pigs collected from one slaughterhouse between October and December 2019. This assay could be very helpful in the rapid differential detection and routine surveillance of EA H1N1, (H1N1)pdm09, and HL H3N2 SIVs in China.


Assuntos
Glicoproteínas de Hemaglutininação de Vírus da Influenza/genética , Vírus da Influenza A Subtipo H1N1/isolamento & purificação , Vírus da Influenza A Subtipo H3N2/isolamento & purificação , Infecções por Orthomyxoviridae/diagnóstico , Animais , China , Modelos Animais de Doenças , Diagnóstico Precoce , Feminino , Vírus da Influenza A Subtipo H1N1/classificação , Vírus da Influenza A Subtipo H1N1/genética , Vírus da Influenza A Subtipo H3N2/classificação , Vírus da Influenza A Subtipo H3N2/genética , Camundongos , Reação em Cadeia da Polimerase Multiplex , Nariz/virologia , Reação em Cadeia da Polimerase em Tempo Real , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Sensibilidade e Especificidade , Suínos
9.
Gynecol Endocrinol ; 37(8): 735-739, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34160336

RESUMO

Objective: To evaluate the efficacy of subcutaneous etonogestrel implants for adenomyosis.Methods: We conducted a clinical observational study of 20 patients suffering from adenomyosis treated with subcutaneous etonogestrel implants from August 2015 to July 2017 and followed up for 36 months. We evaluated the efficacy of subcutaneous etonogestrel implants primarily based on the following indicators: the pictorial blood loss assessment chart (PBAC) for menstrual blood volume, changes in bleeding patterns, the visual analog scale (VAS) pain score for dysmenorrhea, uterine volume, serum cancer antigen 125 (CA125) levels, hemoglobin levels and side effects.Results: During the 3 years of follow-up, subcutaneous etonogestrel implants were removed from six patients, among whom one was diagnosed with endometrial cancer, four had an increased menstrual blood volume, and one entered menopause. In total, 14 patients were treated with subcutaneous etonogestrel implants for 3 years. Among these patients, the number of patients with heavy menstrual bleeding and high PBAC and VAS scores and serum CA125 levels was significantly decreased after implantation compared with that before implantation. In the eight patients with anemia, hemoglobin levels increased gradually. However, the uterine volumes did not significantly change. Bleeding patterns were changed but were tolerable.Conclusion: Subcutaneous etonogestrel implants represent a new option for the clinical treatment of adenomyosis for patients who refuse surgery.

10.
BMC Genomics ; 22(1): 423, 2021 Jun 08.
Artigo em Inglês | MEDLINE | ID: mdl-34103008

RESUMO

BACKGROUND: The study of protein complexes and protein functional modules has become an important method to further understand the mechanism and organization of life activities. The clustering algorithms used to analyze the information contained in protein-protein interaction network are effective ways to explore the characteristics of protein functional modules. RESULTS: This paper conducts an intensive study on the problems of low recognition efficiency and noise in the overlapping structure of protein functional modules, based on topological characteristics of PPI network. Developing a protein function module recognition method ECTG based on Topological Features and Gene expression data for Protein Complex Identification. CONCLUSIONS: The algorithm can effectively remove the noise data reflected by calculating the topological structure characteristic values in the PPI network through the similarity of gene expression patterns, and also properly use the information hidden in the gene expression data. The experimental results show that the ECTG algorithm can detect protein functional modules better.


Assuntos
Mapeamento de Interação de Proteínas , Mapas de Interação de Proteínas , Algoritmos , Análise por Conglomerados , Expressão Gênica , Proteínas/genética , Proteínas/metabolismo
11.
Parasit Vectors ; 14(1): 287, 2021 May 27.
Artigo em Inglês | MEDLINE | ID: mdl-34044867

RESUMO

BACKGROUND: Hard ticks act as arthropod vectors in the transmission of human and animal pathogens and are widely distributed in northern China. The aim of this study is to screen the important tick-borne pathogens (TBPs) carried by hard ticks in Inner Mongolia using metagenomic next-generation sequencing (mNGS) and to estimate the risk of human infection imposed by tick bites. METHODS: The adult Dermacentor nuttalli (n = 203) and Ixodes persulcatus (n = 36) ticks feeding on cattle were collected. The pooled DNA samples prepared from these ticks were sequenced as the templates for mNGS to survey the presence of TBPs at the genus level. Individual tick DNA samples were detected by genus--specific or group-specific nested polymerase chain reaction (PCR) of these TBPs and combined with DNA sequencing assay to confirm the results of mNGS. RESULTS: R. raoultii (45.32%, 92/203), Candidatus R. tarasevichiae (5.42%, 11/203), Anaplasma sp. Mongolia (26.60%, 54/203), Coxiella-like endosymbiont (CLE) (53.69%, 109/203), and Babesia venatorum (7.88%, 16/203) were detected in D. nuttalli, while R. raoultii (30.56%, 11/36), Anaplasma sp. Mongolia (27.80%, 10/36), and CLE (27.80%, 10/36) were detected in I. persulcatus. The double- and triple-pathogen/endosymbiont co-infections were detected in 40.39% of D. nuttalli and 13.89% of I. persulcatus, respectively. The dual co-infection with R. raoultii and CLE (14.29%, 29/203) and triple co-infection with R. raoultii, Anaplasma sp. Mongolia, and CLE (13.79%, 28/203) were most frequent in D. nuttalli. CONCLUSIONS: This study provides insight into the microbial diversity of D. nuttalli and I. persulcatus in Inner Mongolia, China, reporting for the first time that Candidatus R. tarasevichiae had been found in D. nuttalli in China, and for the first time in the world that Anaplasma sp. Mongolia has been detected in I. persulcatus. This study proves that various vertically transmitted pathogens co-inhabit D. nuttalli and I. persulcatus, and indicates that cattle in Inner Mongolia are exposed to several TBPs.


Assuntos
Dermacentor/genética , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Ixodes/genética , Metagenômica , Doenças Transmitidas por Carrapatos/diagnóstico , Anaplasma/genética , Anaplasma/isolamento & purificação , Animais , Vetores Artrópodes/genética , Babesia/genética , Babesiose/diagnóstico , Bovinos , Ixodes/classificação , Ixodidae/genética , Mongólia , Reação em Cadeia da Polimerase , Rickettsia/genética , Infecções por Rickettsia/diagnóstico , Infecções por Rickettsia/veterinária , Doenças Transmitidas por Carrapatos/parasitologia
12.
Adv Mater ; 33(25): e2007073, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33987928

RESUMO

Sonodynamic therapy (SDT) is a non-invasive and highly penetrating treatment strategy under ultrasound irradiation. However, uncertainty in the mechanism of SDT has seriously hindered its future clinical application. Here, the mechanism of SDT enhanced by the wettability of nanoparticles is investigated. Nanoparticles can adsorb and stabilize nanobubbles in liquid, thus enhancing SDT efficiency. The stability of the nanobubbles is positively correlated with the desorption energy of the nanoparticles, which is determined by the wettability of the nanoparticles. This conclusion is verified for mesoporous silica and polystyrene nanoparticles and it is found that nanoparticles with a water contact angle of about 90° possess the largest desorption energy. To further apply this conclusion, thrombus models are constructed on rats and the experimental results demonstrate that nanoparticles with the largest desorption energy have the highest thrombolytic efficiency. It is believed that these findings will help to better understand the SDT mechanism and guide new strategies for rational design of nanoparticles adopted in SDT.

13.
Math Biosci Eng ; 18(3): 2527-2561, 2021 03 15.
Artigo em Inglês | MEDLINE | ID: mdl-33892559

RESUMO

The balance between exploration and exploitation is critical to the performance of a Meta-heuristic optimization method. At different stages, a proper tradeoff between exploration and exploitation can drive the search process towards better performance. This paper develops a multi-objective grasshopper optimization algorithm (MOGOA) with a new proposed framework called the Multi-group and Co-evolution Framework which can archive a fine balance between exploration and exploitation. For the purpose, a grouping mechanism and a co-evolution mechanism are designed and integrated into the framework for ameliorating the convergence and the diversity of multi-objective optimization solutions and keeping the exploration and exploitation of swarm intelligence algorithm in balance. The grouping mechanism is employed to improve the diversity of search agents for increasing coverage of search space. The co-evolution mechanism is used to improve the convergence to the true Pareto optimal front by the interaction of search agents. Quantitative and qualitative outcomes prove that the framework prominently ameliorate the convergence accuracy and convergence speed of MOGOA. The performance of the presented algorithm has been benchmarked by several standard test functions, such as CEC2009, ZDT and DTLZ. The diversity and convergence of the obtained multi-objective optimization solutions are quantitatively and qualitatively compared with the original MOGOA by using two performance indicators (GD and IGD). The results on test suits show that the diversity and convergence of the obtained solutions are significantly improved. On several test functions, some statistical indicators are more than doubled. The validity of the results has been verified by the Wilcoxon rank-sum test.


Assuntos
Gafanhotos , Algoritmos , Animais , Simulação por Computador , Heurística
14.
Zhongguo Zhen Jiu ; 41(3): 269-74, 2021 Mar 12.
Artigo em Chinês | MEDLINE | ID: mdl-33798308

RESUMO

OBJECTIVE: To observe the effect of ginger-separated moxibustion on fatigue state and intestinal flora in patients with chronic fatigue syndrome (CFS). METHODS: A total of 62 patients with CFS were randomly divided into an observation group (31 cases, 3 cases dropped off) and a control group (31 cases, 2 cases dropped off). The patients in the control group were treated with normal diet and moderate exercise; on the basis of the control group, the patients in the observation group were treated with ginger-separated moxibustion at Zhongwan (CV 12), Shenque (CV 8) and Guanyuan (CV 4), 30 min each time, once every other day, three times a week. Both groups were intervened for 4 weeks. Before and after treatment, the fatigue scale-14 (FS-14) was used to observe the improvement of fatigue state, and 16S rRNA detection technology was used to detect the distribution of intestinal flora. RESULTS: Compared before treatment, the FS-14 score was reduced after treatment in the observation group (P<0.01), and the reduction in the observation group was larger than that in the control group (P<0.01). The relative abundance of intestinal flora was similar between the observation group and control group at the phylum and genus level before treatment. After treatment, there was no significant change of intestinal flora in the control group. However, the enterobacteriaceae, corynebacterium, erysipelothrix, actinomycetes were increased in the observation group (P<0.05), and actinomycetes, ruminococcus, lactarius had obvious flora advantages compared with the control group (P<0.05). CONCLUSION: The ginger-separated moxibustion could significantly improve the fatigue state in CFS patients, which may be related to the regulation of intestinal flora structure and the repair of intestinal barrier.


Assuntos
Síndrome de Fadiga Crônica , Microbioma Gastrointestinal , Gengibre , Moxibustão , Pontos de Acupuntura , Síndrome de Fadiga Crônica/terapia , Humanos , RNA Ribossômico 16S
15.
Talanta ; 226: 122165, 2021 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-33676707

RESUMO

Ferrites have attracted considerable attention in biosensor developments owing to their favorable electrochemical and magnetic properties. Speedy and trace analysis can be succeeded by the sensors based on magnetic nanohybrids. In this work, we reported a novel method for one-step synthesis of magnetic ferrites composed of Fe and phthalocyanine. After hybridization with carbonylated multi wall carbon nanotubes, a sensor based on Fe3O4-Pc-CMWCNTs nanocomposites was fabricated for the detection of rosmarinic acid (RA), a bioactive phytochemical. The sensor can be constructed within 30s without any complicated process. A comparison of electrochemical activity between ZnFe2O4-Pc-CMWCNTs and Fe3O4-Pc-CMWCNTs nanohybrids also has been accomplished in this work. Compared with ZnFe2O4-Pc-CMWCNTs based on commonly used ferrites, Fe3O4-Pc-CMWCNTs/MGCE exhibited a higher catalytical ability for the detection of RA. The sensor modified with Fe3O4-Pc-CMWCNTs displayed a low LOD of 0.182 µM with a wide linear range from 0.2 to 400 µM, which was 30 times more sensitive than the one based on ZnFe2O4-Pc-CMWCNTs. The obtained sensor also owned an excellent selectivity, reproducibility, repeatability, and stability, which made it achieve the measurements in plant sample and human serum.


Assuntos
Técnicas Biossensoriais , Nanotubos de Carbono , Cinamatos , Depsídeos , Técnicas Eletroquímicas , Compostos Férricos , Humanos , Indóis , Limite de Detecção , Fenômenos Magnéticos , Reprodutibilidade dos Testes
16.
J Cancer ; 12(4): 1011-1022, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33442400

RESUMO

Despite advancement in the treatment of diffuse large B-cell lymphoma (DLBCL), many patients tend to relapse or become refractory after initial therapy. Therefore, it is essential to identify novel therapeutic targets and drugs, understand the molecular pathogenesis mechanism of DLBCL, and find ways to prevent and treat relapsed or refractory DLBCL. BIX-01294 is a small molecule compound that specifically inhibits EHMT2 activity. In this study, we demonstrate that BIX-01294 triggered the inhibition of human DLBCL cell proliferation, lead to G1 phase arrest via increasing P21 level and reducing cyclin E level. BIX-01294 also induced apoptosis via endogenous and exogenous apoptotic pathways. Moreover, BIX-01294 triggered autophagy and activated ER stress in human DLBCL cells. Furthermore, we showed that both key components of ER stress, ATF3, and ATF4, are required for BIX-01294-induced apoptosis and autophagy. Hence, this study provides new evidence that EHMT2 may be a new therapeutic target, and BIX-01294 may be a potential therapeutic drug for treating DLBCL.

17.
ISA Trans ; 108: 35-47, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32912648

RESUMO

Unmodeled dynamics and output constraint are very often experienced in many physical applications, while few studies on them simultaneously are constructed. This study concentrates on the problem of adaptive multi-dimensional Taylor network dynamic surface control (MTN-DSC) for a class of strict-feedback uncertain nonlinear systems subjected to unmodeled dynamics and output constraint. The purpose is to make this study more practical, and design a controller with simple structure to improve the system performance. A barrier Lyapunov function is used to restrict the system output to within the prescribed constraint. At each step during the backstepping design process, an MTN is applied to approach the generated unknown composite function stemming from the unknown functions and uncertain disturbances, rather than individually; this simplifies the structure and reduces the complexity of the controllers. Meanwhile, the computational burden is further reduced, as only one parameter is adjusted online, which has the minimum number of parameters to be adjusted. Additionally, DSC technique is introduced to eliminate the inherent "explosion of complexity" problem in traditional backstepping design procedure. Furthermore, it is proved that all signals of the closed-loop system are semi-globally uniformly ultimately bounded, and that the output constraint is never violated. The validity of the proposed control scheme is illustrated through the numerical and applied simulation examples.

18.
J Bone Miner Res ; 36(3): 427-435, 2021 03.
Artigo em Inglês | MEDLINE | ID: mdl-33145809

RESUMO

Opportunistic screening for osteoporosis can be performed using low-dose computed tomography (LDCT) imaging obtained for other clinical indications. In this study we explored the CT-derived bone mineral density (BMD) and prevalence of osteoporosis from thoracic LDCT in a large population cohort of Chinese men and women. A total of 69,095 adults (40,733 men and 28,362 women) received a thoracic LDCT scan for the purpose of lung cancer screening between 2018 and 2019, and data were obtained for analysis from the China Biobank Project, a prospective nationwide multicenter population study. Lumbar spine (L1 -L2 ) trabecular volumetric bone mineral density (vBMD) was derived from these scans using quantitative computed tomography (QCT) software and the American College of Radiology QCT diagnostic criteria for osteoporosis were applied. Geographic regional differences in the prevalence of osteoporosis were assessed and the age-standardized, population prevalence of osteoporosis in Chinese men and women was estimated from the 2010 China census. The prevalence of osteoporosis by QCT for the Chinese population aged >50 years was 29.0% for women and 13.5% for men, equating to 49.0 million and 22.8 million, respectively. In women, this rate is comparable to estimates from dual-energy X-ray absorptiometry (DXA), but in men, the prevalence is double. Prevalence varied geographically across China, with higher rates in the southwest and lower rates in the northeast. Trabecular vBMD decreased with age in both men and women. Women had higher peak trabecular vBMD (185.4 mg/cm3 ) than men (176.6 mg/cm3 ) at age 30 to 34 years, but older women had lower trabecular vBMD (62.4 mg/cm3 ) than men (92.1 mg/cm3 ) at age 80 years. We show that LDCT-based opportunistic screening could identify large numbers of patients with low lumbar vBMD, and that future cohort studies are now required to evaluate the clinical utility of such screening in terms of fracture prevention and supporting national health economic analyses. © 2020 The Authors. Journal of Bone and Mineral Research published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research (ASBMR)..


Assuntos
Neoplasias Pulmonares , Osteoporose , Absorciometria de Fóton , Adulto , Idoso , Idoso de 80 Anos ou mais , Densidade Óssea , China/epidemiologia , Detecção Precoce de Câncer , Feminino , Humanos , Masculino , Osteoporose/diagnóstico por imagem , Osteoporose/epidemiologia , Prevalência , Estudos Prospectivos , Tomografia Computadorizada por Raios X
19.
Metabolism ; 115: 154456, 2021 02.
Artigo em Inglês | MEDLINE | ID: mdl-33259834

RESUMO

BACKGROUND: Although abdominal adiposity is associated with an altered cardiometabolic risk profile, the specific contribution of abdominal adipose tissue distribution remains not fully understood. Computed tomography (CT) is a well-established and precise method to measure abdominal adipose tissue distribution. The present study investigated abdominal adiposity assessed by CT in a large-scale Chinese population. METHOD: A total of 59,429 adults who underwent a low dose chest CT for lung cancer screening at one of 13 health checkup centers throughout China were evaluated. Abdominal visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) areas were measured at the center of the 2nd lumbar vertebra with Mindways quantitative CT software using the existing CT dataset without any additional radiation exposure. The ratio of visceral to total adipose tissue (TAT) areas (VAT/TAT ratio) was calculated and expressed as a percentage. Anthropometric indices including body mass index (BMI) and waist circumference were also obtained. RESULTS: BMI, waist circumference, VAT area, SAT area, and the VAT/TAT ratio were 25.0 ±â€¯3.0 kg/m2, 90 ±â€¯8 cm, 194 ±â€¯77 cm2, 85 ±â€¯41 cm2, and 69.5 ±â€¯9.1%, respectively, in men and 23.3 ±â€¯3.1 kg/m2, 79 ±â€¯8 cm, 120 ±â€¯57 cm2, 123 ±â€¯53 cm2, and 48.9 ±â€¯9.7% in women. With increasing age, VAT area and the VAT/TAT ratio increased in both sexes whereas SAT area decreased in men (P < 0.001 for all). After adjustment for BMI and waist circumference, older individuals showed higher VAT area and higher VAT/TAT ratio than younger subjects (P < 0.001 for all). Adjusted VAT areas in participants aged 75 or older was 45 cm2 (95% confidence interval [CI]: 41 cm2, 50 cm2) higher in men and 43 cm2 (95% CI: 37 cm2, 49 cm2) higher in women compared with participants aged 31-44 years. Additionally, differences in VAT area across age groups increased as BMI or waist circumference increased. VAT and SAT areas, but not the VAT/TAT ratio, were positively associated with BMI and waist circumference in every age group. CONCLUSION: In a nationwide study conducted in China, distributions of CT-derived measures of visceral and subcutaneous adiposity were found to vary significantly between sex and age groups. Our study also revealed that the proportion of VAT (an important driver of cardiometabolic risk) could not be predicted from BMI in a Chinese population.


Assuntos
Gordura Abdominal/diagnóstico por imagem , Adiposidade/fisiologia , Obesidade Abdominal/diagnóstico por imagem , Adulto , Idoso , Idoso de 80 Anos ou mais , Índice de Massa Corporal , China , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Valor Preditivo dos Testes , Medição de Risco , Tomografia Computadorizada por Raios X , Circunferência da Cintura/fisiologia
20.
Nanomaterials (Basel) ; 10(11)2020 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-33187078

RESUMO

To develop a synthesis technique providing enhanced control of graphene film quality and uniformity, a systematic characterization and manipulation of hydrocarbon precursors generated during plasma enhanced chemical vapor deposition of graphene is presented. Remote ionization of acetylene is observed to generate a variety of neutral and ionized hydrocarbon precursors, while in situ manipulation of the size and reactivity of carbon species permitted to interact with the growth catalyst enables control of the resultant graphene morphology. Selective screening of high energy hydrocarbon ions coupled with a multistage bias growth regime results in the production of 90% few-to-monolayer graphene on 50 nm Ni/Cu alloy catalysts at 500 °C. Additionally, synthesis with low power secondary ionization processes is performed and reveals further control during the growth, enabling a 50% reduction in average defect densities throughout the film. Mass spectrometry and UV-Vis spectroscopy monitoring of the reaction environment in conjunction with Raman characterization of the synthesized graphene films facilitates correlation of the carbon species permitted to reach the catalyst surface to the ultimate quality, layer number, and uniformity of the graphene film. These findings reveal a robust technique to control graphene synthesis pathways during plasma enhanced chemical vapor deposition.

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