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1.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi ; 38(9): 869-872, 2021 Sep 10.
Artigo em Chinês | MEDLINE | ID: mdl-34487533

RESUMO

OBJECTIVE: To identify the etiology of a patient with severe symptoms of DMD and to trace its pathogenic gene, so as to provide a basis for genetic counseling and clinical intervention. METHODS: Multiple ligation-dependent probe amplification (MLPA) technique was used to analyze exon deletion/repetitive variant of DMD gene, and further analysis was performed by chromosome G-banding, fluorescence in situ hybridization (FISH) and SNP array analysis. RESULTS: The MLPA results of the proband showed that the exon 1-79 of DMD gene were deleted, the G-banding karyotype of blood sample was 46, XY, and the deletion of the short arm of X chromosome was found by FISH. SNP array results showed that 5.8Mb (29 628 158-35 434 714) deletion occurred in the Xp21.2p21.1 region of X chromosome, and the patient was diagnosed as the contiguous deletion syndrome involving the genes of IL1RAPL, MAGEB1-4, ROB, CXorf2, GM, AP3K7IP, FTHL1, DMD, FAM47A, TMEM47, and FAM47B. CONCLUSION: The exact pathogenic site of this family is the deletion of 5.8 Mb (29 628 158-35 434 714) in the Xp21.2p21.1 region of X chromosome, which can be used for prenatal diagnosis. High resolution SNP array technique plays an important role in detecting potential chromosome abnormalities in patients.


Assuntos
Distrofia Muscular de Duchenne , Distrofina/genética , Éxons , Feminino , Deleção de Genes , Humanos , Hibridização in Situ Fluorescente , Distrofia Muscular de Duchenne/diagnóstico , Distrofia Muscular de Duchenne/genética , Gravidez , Diagnóstico Pré-Natal
2.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi ; 38(8): 771-774, 2021 Aug 10.
Artigo em Chinês | MEDLINE | ID: mdl-34365622

RESUMO

OBJECTIVE: To review the clinical data of a fetus with false positive result of non-invasive prenatal testing (NIPT) due to confined placental mosaicism (CPM). METHODS: Amniotic fluid sample was taken from a pregnant women with high risk for chromosome 16 aneuploidy for karyotyping analysis, single nucleotide polymorphism array (SNP array) and interphase fluorescence in situ hybridization (FISH). Genetic testing was also conducted on the fetal and maternal surface of the placenta, root of umbilical cord and fetal skin tissue after induced abortion. RESULTS: Cytogenetic analysis of the amniotic fluid sample yielded a normal karyotype. SNP array revealed mosaicism (20%) of trisomy 16 in the fetus. FISH confirmed the presence of mosaicism (25%) for trisomy 16. After induced labor, all sampled sites of placenta were confirmed to contain trisomy 16 by SNP array, while the analysis of fetal skin tissue yielded a negative result. CONCLUSION: CPM is an important factor for false positive NIPT result. Prenatal identification of CPM and strengthened pregnancy management are important to reduce adverse pregnancy outcomes.


Assuntos
Cromossomos Humanos Par 16 , Mosaicismo , Amniocentese , Cromossomos Humanos Par 16/genética , Análise Citogenética , Feminino , Feto , Humanos , Hibridização in Situ Fluorescente , Biologia Molecular , Placenta , Gravidez , Diagnóstico Pré-Natal , Trissomia/genética
3.
Stem Cell Res Ther ; 12(1): 218, 2021 03 30.
Artigo em Inglês | MEDLINE | ID: mdl-33781342

RESUMO

BACKGROUND: Hepatic ischaemia-reperfusion injury (HIRI) is inevitable in complicated liver surgery and is a major factor leading to postoperative complications and liver dysfunction. Studies have shown that the paracrine mechanisms of stem cell may be essential to tissue repair and functional improvement after transplantation. However, the role of the adipose-derived mesenchymal stem cell secretome (ASC-secretome) in liver regeneration in large animals remains to be determined. METHODS: Twenty-four miniature pigs were subjected to laparoscopic liver ischaemia-reperfusion combined with partial hepatectomy and divided into the following four groups: the saline group, the DMEM group, the ASC group and the ASC-secretome group. Serum and liver tissue samples were collected before the operation and at 1, 3 and 7 days after the operation, and changes in tissue pathology, serum inflammation, liver function, angiogenesis-related factors and liver tissue regeneration-related genes and proteins were evaluated. RESULTS: Detailed histological analysis showed that ASCs and the ASC-secretome changed pathological damage to liver tissue after liver ischaemia-reperfusion combined with partial hepatectomy (1 and 3 days: p < 0.01). Compared with the saline and DMEM control groups, the ASC-secretome group had significantly reduced expression levels of ALP (1 and 3 days: p < 0.05), ALT (1 day: p < 0.01; 3 days: p < 0.05) and AST (1 and 3 days: p < 0.01), which promoted the recovery of liver function. Moreover, detection of the expression levels of TNF-α and IL-1ß (1 day: p < 0.01; 3 days: p < 0.05), IL-6 (1 and 3 days: p < 0.05) and IL-10 (1 and 3 days: p < 0.01) in serum confirmed that the ASC-secretome had obvious anti-inflammatory effects. In addition, the ASC-secretome increased the expression levels of ANG-1 (3 days: p < 0.01), ANG-2 (3 and 7 days: p < 0.01) and VEGF (1 and 7 days: p < 0.05; 3 days: p < 0.01) and promoted angiogenesis during liver regeneration. Moreover, it promoted the mRNA expression of HGF and Cyclin D1 (1 and 3 days: p < 0.01); increased the levels of p-STAT3 (1 and 3 days: p < 0.01), PCNA and Ki67 (1 and 3 days: p < 0.01; 7 days: p < 0.05); inhibited the negative feedback of SOCS3 (1 and 3 days: p < 0.01); and decreased the mRNA expression of TGF-ß (3 days: p < 0.01). The cytokines and growth factors detected in the ASC-secretome included TNF-α, IL-6, IL-1ß, ANG-1, ANG-2, VEGF and b-FGF. CONCLUSION: The ASC-secretome alleviates the inflammatory response induced by ischaemia-reperfusion combined with partial hepatectomy in miniature pigs and promotes liver regeneration.


Assuntos
Hepatopatias , Células-Tronco Mesenquimais , Tecido Adiposo , Animais , Hepatectomia , Isquemia , Fígado , Regeneração Hepática , Reperfusão , Suínos , Porco Miniatura
4.
Transbound Emerg Dis ; 68(2): 445-457, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32568445

RESUMO

Rift Valley fever (RVF) is a mosquito-borne zoonotic disease. Since its first outbreak in 1930, RVF epidemics have caused huge economic losses and public health impacts in Africa. In 2000, RVF became a disease of global concern as it spread to the Arabian Peninsula. In our study, a Geographic Information System-based risk assessment for the occurrence of Rift Valley fever in China was established by means of ecological niche modelling. Based on occurrence records (RVF records from FAO EMPRES-i, vector records from literatures and GBIF) and high-resolution environmental layers, the prediction maps of RVF occurrence probability and distribution of five potential RVF vectors in China were modelled using Maxent. An internal validation was adopted for model verification, and high AUC values were obtained (0.918 for RVF and 0.837-0.992 for vectors). By overlaying the RVF prediction map with the combined RVF vector prediction map using Fuzzy overlay tool ('AND' operator) of ArcMap 10.2, we got the first risk map of possible RVF vector transmission. This map was further overlaid with the latest livestock distribution map ('AND' operator) to generate the second risk map of possible RVF threat to domestic livestock. The south-west border provinces in China, Yunnan, Guangxi and Tibet were predicted to have a high possibility of RVF occurrence. Conditions conducive to the local amplification of RVF also exist in these areas. Temperature seasonality, mean temperature of dry season and precipitation of the driest month were considered as key environmental variables for RVF, and common environmental conditions were found conductive for vectors. It is suggested to establish proper surveillance systems in south-west border areas to minimize the possibility of RVF invasion. Our findings can serve as a valuable reference for prevention measures to be implemented.


Assuntos
Febre do Vale de Rift/epidemiologia , Animais , China/epidemiologia , Culicidae , Surtos de Doenças/prevenção & controle , Vetores de Doenças , Ecossistema , Epidemias , Humanos , Mosquitos Vetores , Vírus da Febre do Vale do Rift , Medição de Risco , Estações do Ano , Tibet
6.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi ; 37(7): 717-720, 2020 Jul 10.
Artigo em Chinês | MEDLINE | ID: mdl-32619249

RESUMO

OBJECTIVE: To detect variants of EXT1 and EXT2 genes among five pedigrees affected with multiple osteochondromas and provide prenatal diagnosis for the families based on the results. METHODS: The EXT1 and EXT2 genes of the probands were analyzed by targeted next generation sequencing (NGS). Suspected pathological variants were validated by Sanger sequencing in the probands, their family members and 200 unrelated healthy controls. Multiple ligation-dependent probe amplification (MLPA) was used to confirm the presence of gross deletions. Prenatal diagnosis was provided for 2 couples carrying pathogenic or likely pathogenic variants. RESULTS: Five variants were detected in the pedigrees, which included EXT1 exon 2-3 deletion, c.1468dupC (p.Leu490ProfsX31), c.2084delC (p.Pro695LeufsX11), and EXT2 c.187delT (p.Phe63SerfsX29) and c.1362T>G (p.Tyr454X). Among these, EXT1 exon 2-3 deletion, c.2084delC (p.Pro695LeufsX11) and EXT2 c.187delT (p.Phe63SerfsX29) were unreported previously. The three novel variants were not found among unaffected members of the pedigree and the 200 healthy controls. Upon prenatal diagnosis, the two fetuses were found to carry the same variants of the the probands. CONCLUSION: Pathological variants of the EXT1 and EXT2 genes probably underlie the multiple osteochondromas among the 5 pedigrees. Prenatal diagnosis based on the results can effectively reduce the birth of further offspring affected with the disease.


Assuntos
Exostose Múltipla Hereditária , Testes Genéticos , Linhagem , Análise Mutacional de DNA , Exostose Múltipla Hereditária/genética , Feminino , Humanos , Mutação , N-Acetilglucosaminiltransferases/genética , Gravidez , Diagnóstico Pré-Natal
7.
Front Cell Dev Biol ; 8: 177, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32266259

RESUMO

Ischemia-reperfusion (IR) is an inevitable complication of liver surgery. Recent studies indicate a critical role of endoplasmic reticulum stress (ERS) in hepatic IR. Mesenchymal stem cells (MSCs) have proven to be an effective tool for tissue regeneration and treatment of various diseases, including that of the liver. However, the mechanisms underlying the therapeutic effects of stem cells on hepatic IR injury (IRI) are still poorly understood, especially in the context of ERS. In this study, we established a porcine model of hepatic IRI and partial hepatectomy, and transplanted the animals with adipose-derived mesenchymal stem cells (ADSCs) isolated from miniature pigs. ADSCs not only alleviated the pathological changes in the liver parenchyma following IRI, but also protected the resident hepatocytes from damage. Mechanistically, the ADSCs significantly downregulated ERS-related proteins, including GRP78, p-eIF2α, ATF6 and XBP1s, as well as the proteins involved in ERS-induced apoptosis like p-JNK, ATF4 and CHOP. Taken together, ADSCs can alleviate hepatic IRI by inhibiting ERS and its downstream apoptotic pathways in the hepatocytes, indicating its therapeutic potential in liver diseases.

8.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi ; 37(5): 559-562, 2020 May 10.
Artigo em Chinês | MEDLINE | ID: mdl-32335886

RESUMO

OBJECTIVE: To carry out genetic analysis for a family with a fetus manifesting bilateral polycystic renal dysplasia and oligohydramnios at 16+ gestational week and a previous history for fetal renal anomaly. METHODS: Ultrasound scan was carried out to detect the morphological changes. Following genetic counselling, the parents had decided to terminate the pregnancy. Fetal kidneys were subjected to histological examination. Target capture and next generation sequencing (NGS) was applied to the abortus to detect potential variants. The results were verified by Sanger sequencing. RESULTS: Histological examination of fetal kidneys revealed cystic changes without cortex, medulla or normal renal structure. NGS has identified a heterozygous c.100+1G>A variant and deletion of exon 3 of the INVS gene, which were respectively inherited from the mother and father. CONCLUSION: Through NGS and Sanger sequencing, the fetus was diagnosed with type II nephronophthisis (NPHP2). Above result can provide guidance for further pregnancy and enforce understanding of clinical features and genetic etiologies for NPHP.


Assuntos
Rim Policístico Autossômico Dominante , Fatores de Transcrição , Ultrassonografia , Feminino , Feto , Testes Genéticos , Heterozigoto , Humanos , Mutação , Rim Policístico Autossômico Dominante/diagnóstico por imagem , Rim Policístico Autossômico Dominante/genética , Gravidez , Deleção de Sequência/genética , Fatores de Transcrição/genética
9.
Pest Manag Sci ; 76(9): 3096-3107, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32281209

RESUMO

BACKGROUND: Intense studies have been carried out on the effects of climate change on vector-borne diseases and vectors. Culex pipiens pallens and Culex pipiens quinquefasciatus are two medically concerned mosquito species in temperate and tropical areas, which serve as important disease-transmitting pests of a variety of diseases. The ongoing geographical expansion of these mosquitoes has brought an increasing threat to public health. RESULTS: Based on mosquito occurrence records and high-resolution environmental layers, an ecological niche model was established to model their current and future potential distribution in China. Our model showed that the current suitable area for Cx. p. pallens is distributed in the central, eastern and northern parts of China, while Cx. p. quinquefasciatus is distributed in vast areas in southern China. Under future climate change scenarios, both species are predicted to expand their range to varying degrees and RCP 8.5 provides the largest expansion. Northward core shifts will occur in ranges of both species. Environmental variables which have significant impact on the distribution of mosquitoes were also revealed by our model. CONCLUSION: Severe habitat expansion of vectors is likely to occur in the future 21st century. Our models mapped the high-risk areas and risk factors which needs to be paid attention. The results of our study can be referenced in further ecological surveys and will guide the development of strategies for the prevention and control of vector-borne diseases. © 2020 Society of Chemical Industry.


Assuntos
Culex , Animais , China , Mudança Climática , Ecossistema , Mosquitos Vetores
10.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi ; 37(4): 397-400, 2020 Apr 10.
Artigo em Chinês | MEDLINE | ID: mdl-32219821

RESUMO

OBJECTIVE: To explore the genetic etiology of three pedigrees with a gestational history of fetal renal anomalies. METHODS: Peripheral venous blood or skin samples were derived from the probands of the three pedigrees. Copy number variation sequencing (CNV-seq) was applied to detect alterations of genome CNVs. RESULTS: The patient from pedigree 1 and the fetuses from pedigrees 2 and 3 all carried a heterozygous 17q12 deletion, with the size ranging from 1.4 Mb to 1.48 Mb encompassing the HNF1B gene. CONCLUSION: The diagnosis of 17q12 microdeletion may be difficult during fetal period for its variable phenotypes. Alterations of chromosomal copy numbers need to be excluded in such patients.


Assuntos
Deleção Cromossômica , Cromossomos Humanos Par 17/genética , Variações do Número de Cópias de DNA , Testes Genéticos , Linhagem , Feto , Fator 1-beta Nuclear de Hepatócito/genética , Humanos , Fenótipo
11.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi ; 37(2): 123-126, 2020 Feb 10.
Artigo em Chinês | MEDLINE | ID: mdl-32034735

RESUMO

OBJECTIVE: To detect potential variants of COL1A1 gene in five Chinese pedigrees affected with osteogenesis imperfecta (OI) and provide prenatal diagnosis for a fetus at 11th gestational week. METHODS: The coding regions and exon/intron boundaries of 225 genes associated with bone diseases were subjected to targeted capture and next generation sequencing (NGS). Suspected mutations were verified with Sanger sequencing in the probands, unaffected relatives and 100 unrelated healthy controls. Prenatal diagnosis for a high-risk fetus was carried out by Sanger sequencing. RESULTS: The probands of the pedigrees 1-5 have respectively carried c.3226G>A (p.Gly1076Ser), c.579delT (p.Gly194Valfs*71), c.2911-2912insAG (p.Gly971Glufs*138), c.3037G>A (p.Gly1013Arg) and c.642+5G>A variants of the COL1A1 gene. For pedigree 1, the same variant was not found in the fetus. c.3037G>A (p.Gly1013Arg) and c.2911-2912insAG (p.Gly971Glufs*138) were not reported previously. CONCLUSION: Mutations of the COL1A1 gene probably underlie the OI in the five pedigrees. Combined NGS and Sanger sequencing can provide an effective and accurate method for the genetic and prenatal diagnosis of OI.


Assuntos
Colágeno Tipo I/genética , Osteogênese Imperfeita , Grupo com Ancestrais do Continente Asiático , Feminino , Humanos , Mutação , Osteogênese Imperfeita/genética , Linhagem , Gravidez
12.
Mol Genet Genomic Med ; 8(3): e1128, 2020 03.
Artigo em Inglês | MEDLINE | ID: mdl-31960627

RESUMO

BACKGROUND: Waardenburg syndrome (WS) is a dominantly inherited, genetically heterogeneous auditory-pigmentary syndrome characterized by nonprogressive sensorineural hearing loss and iris discoloration. This study aimed to investigate the underlying molecular pathology in Chinese WS families. METHODS: A total of 13 patients with Waardenburg syndrome type II (WS2) from six unrelated Chinese families were enrolled. We investigated the mutation profile of genes related to congenital deafness in these families through a targeted sequencing technology and validated the candidate variants by Sanger sequencing. RESULTS: We identified six novel variants in microphthalmia-associated transcription factor (MITF) and SRY-box 10 (SOX10), which were predicted to be disease causing by in silico analysis. Our results showed that mutations in SOX10 and MITF are two major causes of deafness associated with WS, and de novo mutations were frequently found in probands with SOX10 mutations but not in those with MITF mutations. CONCLUSION: Results showed that targeted next-generation sequencing (NGS) enabled us to detect disease-causing mutations with high accuracy, stability, speed and throughput. Our study extends the pathogenic mutation spectrum of MITF and SOX10.


Assuntos
Fator de Transcrição Associado à Microftalmia/genética , Mutação , Fatores de Transcrição SOXE/genética , Síndrome de Waardenburg/genética , Adolescente , Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Síndrome de Waardenburg/patologia
13.
Stem Cells Int ; 2019: 2489584, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31827526

RESUMO

Aim: To study the anti-inflammatory and liver regenerative effects of adipose-derived mesenchymal stem cells (ADSCs) on a porcine model of ischemia-reperfusion (IR) and hemihepatectomy. Methods: Eighteen healthy Bama miniature pigs were randomly divided into the sham-operated (sham), untreated IR injury (IRI), and ADSC-transplanted (ADSC) groups. Hepatic IR was established by laparoscopic hemihepatectomy. ADSCs were transplanted directly into the liver parenchyma after the surgery. Hepatic inflammation and liver regeneration were evaluated by histopathological examination and assessment of relevant cytokines and other factors. Results: ADSC transplantation successfully ameliorated the IRI-induced histopathological damage and the high levels of pro-inflammatory cytokines like IL-1ß, IL-6, and TNF-α. In addition, the ADSCs enhanced the expression of the anti-inflammatory IL-10, regenerative factors including HGF, Cyclin D1, and proliferating cell nuclear antigen (PCNA), and angiogenic factors like VEGF, ANG-1, and ANG-2. Conclusions: ADSCs attenuated the hepatic IRI-induced inflammatory response and promoted liver regeneration.

14.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi ; 36(12): 1153-1157, 2019 Dec 10.
Artigo em Chinês | MEDLINE | ID: mdl-31813136

RESUMO

OBJECTIVE: The phenotype and genetics of three patients with autosomal recessive polycystic kidney disease (ARPKD) at childhood, teenage and advanced age were analyzed. METHODS: Next generation sequencing (NGS) was applied to all the probands. PCR and Sanger sequencing were used to verify the suspicious gene variants screened by NGS in the probands and their family members, and one of the family got prenatal diagnosis. RESULTS: Through NGS, PCR and Sanger sequencing, the 5-yr proband in pedigree 1 was shown to carry compound heterozygous variants of c.5935G>A(p.G1979R) and c.5428G>T(p.E1810X) of PKHD1, originated from his parents; In pedigree 2, the 17-ys proband was detected with c.5512T>C(p.Y1838H) and c.5935G>A(p.G1979R) variants of PKHD1 orginated from her parents, and her mother also got prenatal diagnosis during the second trimester; In pedigree 3, the 70-ys female proband was found with variants c.11314C>T (p.R3772X) and c.3860T>G (p.V1287G) of PKHD1. CONCLUSION: The three pedigrees were diagnosed as ARPKD caused by PKHD1 variants. Five types of variants were detected, c.5935G>A and c.11314C>T were the known pathogenic variants, while c.5512T>C, c.5428G>T and c.3860T>G were not reported previously. Considering the complexity of the genetics and phenotypes of the cystic renal diseases, genetic diagnosis is crucial to give accurate etiological diagnosis, which may benefit the clinic management.


Assuntos
Rim Policístico Autossômico Recessivo/genética , Receptores de Superfície Celular/genética , Adolescente , Idoso , Pré-Escolar , Feminino , Humanos , Masculino , Mutação , Fenótipo , Gravidez
15.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi ; 36(12): 1179-1182, 2019 Dec 10.
Artigo em Chinês | MEDLINE | ID: mdl-31813142

RESUMO

OBJECTIVE: To analyze variants of RUNX2 gene in two pedigrees affected with cleidocranial dysplasia and provide prenatal diagnosis for them. METHODS: For the two probands, the coding sequences of the RUNX2 gene were analyzed with PCR and bidirectional Sanger sequencing. To verify the results, peripheral blood samples were collected from their parents and 100 healthy controls. For family 1, umbilical cord blood was also collected for prenatal genetic diagnosis. RESULTS: In family 1, the proband and the fetus both carried a heterozygous c.578G>C (p.Arg193Pro) mutation. For family 2, the proband was found to carry a heterozygous c.909C>A (p.Tyr303X) mutation. The same mutations were not found among their parents and 100 healthy controls. Neither mutation was reported previously. CONCLUSION: Variants of the RUNX2 gene probably underlie the cleidocranial dysplasia in both pedigrees. The results enabled prenatal diagnosis for the affected family.


Assuntos
Displasia Cleidocraniana/diagnóstico , Subunidade alfa 1 de Fator de Ligação ao Core/genética , Displasia Cleidocraniana/genética , Éxons , Feminino , Humanos , Mutação , Linhagem , Gravidez , Diagnóstico Pré-Natal
16.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi ; 36(10): 1028-1030, 2019 Oct 10.
Artigo em Chinês | MEDLINE | ID: mdl-31598953

RESUMO

OBJECTIVE: To carry out genetic testing for a family with two pregnancies affected with hydrops fetalis and dilated cardiomyopathy (DCM) of the fetus. METHODS: DNA was extracted from fetal tissue as well as peripheral blood samples from the couple. Single nucleotide polymorphism array (SNP array) and next-generation sequencing (NGS) were carried out to screen potential mutation. Suspected mutation was validated with PCR and Sanger sequencing. RESULTS: The manifestation of fetal echocardiography was consistent with DCM. No obvious abnormality was found by SNP array analysis. A hemizygous c.481G>A (p.G161R) mutation of the TAZ gene was detected in the male fetus by NGS and confirmed by Sanger sequencing. The mutation was inherited from his mother. CONCLUSION: Barth syndrome due to the c.481G>A mutation of the TAZ gene probably underlies the recurrent hydrops fetalis and fetal DCM in this family.


Assuntos
Cardiomiopatia Dilatada/genética , Hidropisia Fetal/genética , Fatores de Transcrição/genética , Síndrome de Barth/genética , Ecocardiografia , Feminino , Testes Genéticos , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Masculino , Mutação , Gravidez
17.
Biomed Pharmacother ; 120: 109453, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31561069

RESUMO

AIM: To compare and evaluate the hepatoprotective effect of liver parenchyma injection of ADSCs and portal vein injection of HRS in laparoscopic hepatic ischemia reperfusion combined with hepatectomy injury in miniature pigs. METHODS: Eighteen miniature pigs were randomly assigned to IRI group, HRS group and ADSCs group. HRS was injected through the portal vein 10 min before reperfusion, 1 d, 2 d, and 3 d after surgery. ADSCs were injected into liver parenchyma after hepatectomy. The serum and liver tissue samples were collected at different time points (preoperative, and postoperative at 1 d, 3 d and 7 d). RESULTS: Compared with the IRI group, both ADSCs and HRS groups can promote liver function recovery, reduce oxidative stress, reduce inflammation, and promote liver regeneration. Compared with HRS, ALT and TBIL in ADSCs group were significantly decreased at 3 d, and AST was significantly reduced at 1 d. The activities of SOD and GSH-Px in ADSCs group were significantly higher than that in HRS group, but the MDA level in HRS group was markedly lower than that in ADSCs group at 1 d. IL-1ß was significantly lower in the ADSCs group than in the HRS group at 1 day after operation. The expressions of HGF and PCNA were significantly higher than that in the HRS group at 3 day after surgery. CONCLUSION: Our study has demonstrated that HRS and ADSCs have significant hepatoprotective effects in miniature pigs after HIRI and hepatectomy injury. However, liver parenchyma injection of ADSCs is more beneficial to the recovery of liver function than portal vein injection of HRS.


Assuntos
Hepatectomia , Hidrogênio/farmacologia , Regeneração Hepática/efeitos dos fármacos , Traumatismo por Reperfusão/metabolismo , Células-Tronco/metabolismo , Adipócitos/metabolismo , Animais , Apoptose , Inflamação/tratamento farmacológico , Fígado/efeitos dos fármacos , Testes de Função Hepática , Modelos Animais , Estresse Oxidativo/efeitos dos fármacos , Substâncias Protetoras , Traumatismo por Reperfusão/induzido quimicamente , Suínos
19.
Pest Manag Sci ; 75(11): 3076-3083, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30919547

RESUMO

BACKGROUND: Aedes aegypti is one of the most important mosquito species and is a common disease-transmitting pest in tropical areas. Various infectious arbovirus diseases can be transmitted by Ae. aegypti. With ongoing global climate change, we are facing an increasing public health threat from the rapid spread of disease vectors into wider geographical areas. To better understand the current ecological niche range and possible future expansion of Ae. aegypti, an ecological niche modelling approach was adopted to predict its current and future potential habitat in Taiwan, China. RESULTS: Based on observed occurrence records and environmental layers reflecting climate and land-use conditions, predictions with a high resolution of 30 arcsec (approx. 1 × 1 km) were made by our model. Ae. aegypti was predicted to expand its habitat in varying degrees out of its current niche range under different climate scenarios for the future 21st century. Winter temperature and dry season precipitation were considered as important predictors among climate variables. Croplands, pasture, forested lands and urban lands were important land-use variables. CONCLUSION: Ae. aegypti is expected to establish new habitats out of its current niche range under the trend of global climate change. The extent of habitat expansion varies under different climate scenarios. Appropriate measures should be taken to prevent its expansion to a broader scale. Our study has important strategic implications for mosquito surveillance and the prevention and control of mosquito-borne diseases. © 2019 Society of Chemical Industry.


Assuntos
Aedes/fisiologia , Distribuição Animal , Mudança Climática , Mosquitos Vetores/fisiologia , Animais , Infecções por Arbovirus/transmissão , Arbovírus , China , Ecossistema , Taiwan
20.
Cancer Med ; 8(4): 1919-1929, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-30843665

RESUMO

BACKGROUND: Circadian positive feedback loop (CPFL) genes (CLOCK, BAML1, and NPAS2) have been implicated in cancer initiation and progression. The purpose of this study was to explore the effects of single-nucleotide polymorphisms (SNPs) in CPFL genes on prognosis of gastric cancer (GC) patients. METHODS: Nine functional SNPs from the three CPFL genes were genotyped in a cohort of 704 GC patients undergoing resection. Multivariate Cox regression model and Kaplan-Meier curve were used for prognosis analysis. RESULTS: Among the nine SNPs, rs11133399 in CLOCK, rs1044432 and rs2279284 in BAML1 were significantly associated with GC overall survival and recurrence-free survival. The unfavorable genotypes of these SNPs showed a cumulative effect on GC prognosis. Multivariate assessment model indicated that these SNPs, in conjunction with clinical variables, enhanced the power to predict GC prognosis. In addition, survival tree analysis revealed the genotype of rs11133399 as a primary risk factor contributing to the prognosis of GC patients. Functional assays showed that the G allele in rs11133399 significantly enhanced luciferase reporter activity than A allele. Immunohistochemical analysis further demonstrated that the genotype of rs11133399 was significantly associated with the expression level of CLOCK in GC tissues, suggesting that this SNP might affect the prognosis of GC through its influence on the expression of CLOCK gene. CONCLUSIONS: Our data indicate that SNPs in CPFL genes might contribute to the clinical outcome of GC through their impact on gene expression. Further studies are needed to elucidate its underlying molecular mechanisms.


Assuntos
Peptídeos e Proteínas de Sinalização do Ritmo Circadiano/genética , Polimorfismo de Nucleotídeo Único , Neoplasias Gástricas/genética , Fatores de Transcrição ARNTL/genética , Idoso , Fatores de Transcrição Hélice-Alça-Hélice Básicos/genética , Proteínas CLOCK/genética , China/epidemiologia , Relógios Circadianos/genética , Epistasia Genética , Feminino , Regulação Neoplásica da Expressão Gênica , Predisposição Genética para Doença , Genótipo , Humanos , Estimativa de Kaplan-Meier , Masculino , Pessoa de Meia-Idade , Proteínas do Tecido Nervoso/genética , Prognóstico , Regiões Promotoras Genéticas/genética , Neoplasias Gástricas/mortalidade , Neoplasias Gástricas/cirurgia
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