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Diagn Microbiol Infect Dis ; 69(1): 7-11, 2011 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-21146708


We evaluated the Kanagawa hemolytic test and tdh gene test for accuracy in identifying pathogenic Vibrio parahaemolyticus isolates in Shanghai. One hundred and seventy-two V. parahaemolyticus isolates were collected from diarrhea patients, freshly harvested sea fish, or fresh water samples. Statistical data for the Kanagawa hemolytic test and tdh gene test were compared. There were 83.51% isolates (81/97) from patients and 22.22% isolates (10/45) from sea-fish positive for the tdh gene. However, none of 30 isolates from fresh water samples were tdh-positive. Positive Kanagawa hemolytic tests were obtained in 88.66%, 46.67%, and 76.67% of isolates, which were from patients, sea fish, and fresh water samples, respectively. Positive rates of the Kanagawa hemolytic tests and the tdh gene tests were significantly different in isolates from those 3 sources (P < 0.001). The tdh gene test showed higher specificity than the Kanagawa hemolytic test on identifying pathogenic V. parahaemolyticus isolates in Shanghai, China.

Técnicas Bacteriológicas/métodos , Peixes/microbiologia , Proteínas Hemolisinas/genética , Proteínas Hemolisinas/metabolismo , Vibrioses/diagnóstico , Vibrio parahaemolyticus/isolamento & purificação , Microbiologia da Água , Animais , Toxinas Bacterianas/genética , Toxinas Bacterianas/metabolismo , China , DNA Bacteriano/genética , Hemólise , Humanos , Sensibilidade e Especificidade , Vibrio parahaemolyticus/genética , Vibrio parahaemolyticus/patogenicidade
Indian J Med Res ; 129(6): 701-6, 2009 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-19692753


BACKGROUND & OBJECTIVE: Fluoroquinolone has a broad spectrum of antimicrobial activity, and is widely used for gonorrhoea treatment. However, its efficacy can be compromised by the drug-resistance property of Neisseria gonorrhoeae isolates. Most resistant cases of N. gonorrhoeae are associated with mutations in the quinolone-resistance-determining-region (QRDR) within genes of gyrA and parC. This study was undertaken to describe resistance profile of N. gonorrhoeae to fluoroquinolones in Shanghai, P.R. of China, and also associated resistance mutations in gyrA and parC. METHODS: Eighty N. gonorrhoeae isolates were collected from Shanghai Skin Disease & Sexually Transmitted Disease Hospital or DongFang Hospital during April 2005 to April 2006 in Shanghai, P.R. of China. The minimum inhibitory concentrations (MIC) of fluoroquinolones for these isolates were determined by an agar dilution method. Mutation patterns within gyrA and parC were determined by direct sequencing or by using established restriction fragment length polymorphisms (RFLP) methods. RESULTS: Ninety five per cent (76 of 80) of isolates were resistant, 3.75 per cent (3 of 80) intermediate resistant, and 1.25 per cent (1 of 80) were sensitive to fluoroquinolone drug ciprofloxacin. Sequencing and RFLP analysis of gyrA and parC revealed that all resistant isolates had dual mutations of S91F and D95A/G/N in gyrA. Some isolates had an extra mutation within parC either of D86N, S87N or E91A/G. Mutation patterns for gyrA and parC were significantly (P<0.05) associated with MICs level. INTERPRETATION & CONCLUSION: Mutations of S91F and D95A/G/N in gyrA combined with S87N in parC was the most prevalent mutation pattern of fluoroquinolone resistant N. gonorrhoeae isolates. This mutation pattern was associated with a high level of quinolone resistance (MIC >16.0 microg/ml) which can serve as a maker for quinolone-resistance prediction in Shanghai, P.R. of China.

Antibacterianos , Farmacorresistência Bacteriana/genética , Fluoroquinolonas , Mutação , Neisseria gonorrhoeae , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , China , Fluoroquinolonas/farmacologia , Fluoroquinolonas/uso terapêutico , Humanos , Testes de Sensibilidade Microbiana , Neisseria gonorrhoeae/efeitos dos fármacos , Neisseria gonorrhoeae/genética , Polimorfismo de Fragmento de Restrição , Análise de Sequência de DNA