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1.
Sci Rep ; 10(1): 3002, 2020 Feb 14.
Artigo em Inglês | MEDLINE | ID: mdl-32060371

RESUMO

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

2.
J Microbiol ; 58(2): 142-152, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31993988

RESUMO

Pleurotus pulmonarius, a member of the Pleurotaceae family in Basidiomycota, is an edible, economically important mushroom in most Asian countries. In this study, the complete mitochondrial genomes (mtDNA) of three P. pulmonarius strains - two monokaryotic commercial (J1-13 and ZA3) and one wild (X1-15) - were sequenced and analyzed. In ZA3 and X1-15, the mtDNA molecule was found to be a single circle of 68,305 bp and 73,435 bp, respectively. Both strains contain 14 core protein-coding genes and two ribosomal RNA (rRNA) subunit genes. The ZA3 strain has 22 transfer RNA (tRNA) genes and nine introns: eight in cytochrome c oxidase subunit 1 (coxl), and one in the rRNA large subunit (rnl). Monokaryotic J1-13 and ZA3 mtDNAs were found to be similar in their structure. However, the wild strain X1-15 contains 25 tRNA genes and only seven introns in coxl. Open reading frames (ORFs) of ZA3/J1-13 and X1-15 encode LAGLIDADG, ribosomal protein S3, and DNA polymerase II. In addition, mtDNA inheritance in J1-13, ZA3, and X1-15 was also studied. Results showed that the mtDNA inheritance pattern was uniparental and closely related to dikaryotic hyphal location with respect to the parent. Results also show that mtDNA inheritance is influenced by both the parental nuclear genome and mitogenome in the zone of contact between two compatible parents. In summary, this analysis provides valuable information and a basis for further studies to improve our understanding of the inheritance of fungal mtDNA.

3.
Food Chem ; 311: 125878, 2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-31771910

RESUMO

ß-Carotene is a natural nutrient that serves as a natural food colorant. However, the weak physical stability restricts its development in food industrial production. Here, the influences of a variety of external environmental conditions on the stability of ß-carotene enriched zein-carboxymethyl chitosan (CMCS)-tea polyphenols (TP) ternary composite nanoparticles were investigated. Compared with zein unitary and zein-CMCS binary complexes, it was interesting to note that ternary complexes had the best stability against color fading and there was little impact on its nanoparticle size during storage with change in temperature. Besides excellent antioxidant properties, ternary complexes were extremely effective in inhibiting ß-carotene color degradation when exposed to ultraviolet light. Based on our results, the novel zein-CMCS-TP nanoparticles are expected to be an effective delivery system to encapsulate hydrophobic bioactive compounds, which is a promising approach to improve their storage stability against external environmental stresses.

4.
Sci Total Environ ; 703: 134977, 2020 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-31757553

RESUMO

The optimization of more sustainable fertilization practice to relieve phosphorus (P) resource scarcity and increase P fertilizer utilization, a better understanding of the regulatory roles of microbes in P mobilization is urgently required to reduce P input. The genes phoD and pqqC are responsible for regulating organic and inorganic P mobilization, respectively. Using high-throughput sequencing, the corresponding bacterial communities harbored by these genes were determined. We conducted a 4-year rice-rice-crop rotation to investigate the responses of phoD- and pqqC-harboring bacterial communities to the partial replacement of inorganic P fertilizer by organic manure with reduced P input. The results showed that a combination of organic and inorganic fertilization maintained high rice yield, and also produced a more complex and stable phosphate mobilizing bacterial community, which contributed to phosphatase activities more than their gene abundances in the model analysis. Compared with the conventional mineral fertilization, organic-inorganic fertilization with the reduced P input slightly increased pqqC gene abundance while significantly enhanced the abundance of phoD-harboring bacteria, especially the genera Bradyrhizobium and Methylobacterium known as potential organic P mineralizers which can maintain high rice production. Moreover, the increased pH was the most impactful factor for the phoD- and pqqC-harboring bacterial communities, by promoting microbial P turnover and greatly increasing bioavailable P pools (H2O-Pi and NaHCO3-Pi, NaOH-Pi) in this P-deficient paddy soil. Hence, our study demonstrated that the partial replacement of mineral P with organic manure could reshape the inorganic phosphate solubilizing and alkaline-phosphomonoesterase encoding bacterial communities towards more resilient and effective to the high P utilization and productivity over intense cultivation, providing insights into the potential of soil microbes in the efficient management of agricultural P fertilization.

5.
Midwifery ; 81: 102588, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31805429

RESUMO

OBJECTIVES: There is very limited research on maternal health needs and no reliable validated research tools have been developed. The purpose of this study was to develop and test a Maternal Health Needs Scale (MHNS) in Chinese maternal women. METHODS: Using Maslow's hierarchy of needs as the theoretical model, the pool of items regarding maternal health needs was developed through existing literature, expert evaluation and modification, and a pilot study's feedback. One hundred pregnant women without pregnancy complications were recruited from the outpatient department of a tertiary hospital in China for the pilot study to select appropriate items and form the MHNS. The reliability and validity of MHNS was tested using data collected in two tertiary hospitals in China, with 1304 pregnant women enrolled and 1050 of them with completed questionnaires. The MHNS was modified and finalized according to the analyzed results. RESULTS: The final version of MHNS had six dimensions with 23 items. The Cronbach's alpha coefficient was 0.94 for the total scale; the test-retest reliability was 0.85; and the split-half reliability was 0.85. The MHNS showed great validity: model Chi-Square was 693.07; Minimum Discrepancy (Chi-square Mean/Degree of Freedom) was 3.22; Root Mean Square Error of Approximation was 0.07; Comparative Fit Index was 0.94; and Tucker-Lewis Index was 0.93. The item with the highest score of maternal health needs was knowing the symptoms and coping strategies when the baby is abnormal; the item with the lowest score of MHNS was knowledge of sexual intercourse during pregnancy. CONCLUSIONS: The MHNS is reliable and valid for assessing maternal health needs and could be used in future studies for the health needs of Chinese maternal women without pregnancy complications or cognitive impairment.

6.
Nanoscale ; 12(3): 1500-1512, 2020 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-31859311

RESUMO

Atomically thin black phosphorus (BP) field-effect transistors have excellent potential for sensing applications. However, commercial scaling of PFET sensors is still in the early stage due to various technical challenges, such as tedious fabrication, low response% caused by rapid oxidation, non-ideal response output (spike/bidirectional), and large device variation due to poor control over layer thickness among devices. Attempts have been made to address these issues. First, a theoretical model for response% dependence on the number of layers is developed to show the role of atomically thin BP for better responses. A position-tracked, selected-area-exfoliation method has been developed to rapidly produce thin BP layers with a narrow distribution (∼1-7 layers), which can harness excellent gate control over the PFET channel. The typical current on/off ratio is in the range of ∼300-500. The cysteine-modified Al2O3-gated PFET sensors show high responses (∼30-900%) toward a wide detection range (∼1-400 ppb) of lead ions in water with a typical response time of ∼10-30 s. A strategy to minimize device variation is proposed by correlating PFETs' on/off ratio with sensitivity parameters. The thickness variation of the gate oxide is investigated to explain non-ideal and ideal response transient kinetics.

7.
Sci Rep ; 9(1): 18420, 2019 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-31804513

RESUMO

Resveratrol and quercetin have effects on polycystic ovary syndrome (PCOS). Hence, resveratrol combined with quercetin may have better effects on it. However, because of the limitations in animal and human experiments, the pharmacological and molecular mechanism of quercetin-resveratrol combination (QRC) remains to be clarified. In this research, a systematic pharmacological approach comprising multiple compound target collection, multiple potential target prediction, and network analysis was used for comparing the characteristic of resveratrol, quercetin and QRC, and exploring the mechanism of QRC. After that, four networks were constructed and analyzed: (1) compound-compound target network; (2) compound-potential target network; (3) QRC-PCOS PPI network; (4) QRC-PCOS-other human proteins (protein-protein interaction) PPI network. Through GO and pathway enrichment analysis, it can be found that three compounds focus on different biological processes and pathways; and it seems that QRC combines the characteristics of resveratrol and quercetin. The in-depth study of QRC further showed  more PCOS-related biological processes and pathways. Hence, this research not only offers clues to the researcher who is interested in comparing the differences among resveratrol, quercetin and QRC, but also provides hints for the researcher who wants to explore QRC's various synergies and its pharmacological and molecular mechanism.

8.
Zhen Ci Yan Jiu ; 44(9): 668-71, 2019.
Artigo em Chinês | MEDLINE | ID: mdl-31532137

RESUMO

OBJECTIVE: To investigate the effective of acupoint thread-embedding therapy for ataxia children with cerebral palsy. METHODS: A total of 70 ataxia children with cerebral palsy and a Gross Motor Function Measure (GMFM, Dimension B) score of <35 were enrolled and randomly divided into thread-embedding group and control group, with 35 children in each group, and 2 children in the thread-embedding group were lost to follow-up. The children in the control group were given routine rehabilitation treatment, including physical therapy, spleen-strengthening, kidney-nourishing, and Governor Vessel-regulating massage, vibroacoustic therapy, and scalp acupuncture, and those in the thread-embedding group were given thread-embedding therapy at the acupoints of Jianyu (LI15), Jianliao (SJ14), Tianzong (SI11), Tianshu (ST25), and HuatuoJiaji points (C4, L1 and L4) in addition to the treatment in the control group, with 6-8 acupoints selected each time, once a week. Each course of treatment was 4 weeks, with an interval of one week between two courses of treatment, and the children were treated for 3 courses. Level of sitting scale (LSS), incurvation reflex, and GMFM score were recorded to evaluate the improvement in sitting ability and clinical outcome. RESULTS: Both groups had significant improvements in LSS, incurvation reflex, and GMFM (Dimension A and B) score after treatment (P<0.01), and the thread-embedding group had significantly greater improvements than the control group (P<0.01). The thread-embedding group had a significantly higher overall response rate than the control group (90.9% [30/33] vs 68.5% [24/35], P<0.01). CONCLUSION: In addition to routine rehabilitation treatment, acupoint thread-embedding therapy can effectively suppress primitive reflex in ataxia children with cerebral palsy and significantly promote their sitting ability, and therefore, it is an effective acupuncture treatment method for ataxia children with cerebral palsy.


Assuntos
Pontos de Acupuntura , Paralisia Cerebral , Ataxia , Paralisia Cerebral/terapia , Criança , Humanos , Postura Sentada
9.
Nat Commun ; 10(1): 3233, 2019 07 19.
Artigo em Inglês | MEDLINE | ID: mdl-31324787

RESUMO

MAVS is essential for antiviral immunity, but the molecular mechanisms responsible for its tight regulation remain poorly understood. Here, we show that NLK inhibits the antiviral immune response during viral infection by targeting MAVS for degradation. NLK depletion promotes virus-induced antiviral cytokine production and decreases viral replication, which is potently rescued by the reintroduction of NLK. Moreover, the depletion of NLK promotes antiviral effects and increases the survival times of mice after infection with VSV. NLK interacts with and phosphorylates MAVS at multiple sites on mitochondria or peroxisomes, thereby inducing the degradation of MAVS and subsequent inactivation of IRF3. Most importantly, a peptide derived from MAVS promotes viral-induced IFN-ß production and antagonizes viral replication in vitro and in vivo. These findings provide direct insights into the molecular mechanisms by which phosphorylation of MAVS regulates its degradation and influences its activation and identify an important peptide target for propagating antiviral responses.


Assuntos
Proteínas Adaptadoras de Transdução de Sinal/imunologia , Imunidade Inata/imunologia , Interferon beta/imunologia , Proteínas Serina-Treonina Quinases/imunologia , Proteínas Adaptadoras de Transdução de Sinal/genética , Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Animais , Células HCT116 , Células HEK293 , Humanos , Imunidade Inata/genética , Interferon beta/metabolismo , Camundongos Endogâmicos C57BL , Camundongos Knockout , Fosforilação , Ligação Proteica , Proteínas Serina-Treonina Quinases/genética , Proteínas Serina-Treonina Quinases/metabolismo , Transdução de Sinais/imunologia , Células Vero , Vírus da Estomatite Vesicular Indiana/imunologia , Vírus da Estomatite Vesicular Indiana/fisiologia
10.
Am J Clin Nutr ; 110(3): 675-684, 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31268126

RESUMO

BACKGROUND: Airborne pollutants have collectively been classified as a known human carcinogen and, more broadly, affect the health of hundreds of millions of people worldwide. Benzene is a frequent component of air pollution, and strategies to protect individuals against unavoidable exposure to this and other airborne carcinogens could improve the public's health. Earlier clinical trials in Qidong, China, demonstrated efficacy in enhancing the detoxication of benzene using a broccoli sprout beverage. OBJECTIVES: A randomized, placebo-controlled, multidose trial of a broccoli sprout beverage was designed to determine the lowest effective concentration that enhances benzene detoxication adjudged by enhanced excretion of the urinary biomarker, S-phenylmercapturic acid (SPMA). METHODS: Following informed consent, 170 subjects were randomly assigned in 5 blocks of 34 each to drink either a placebo beverage (n = 55) or 1 of 3 graded concentrations of a broccoli sprout beverage [full (n = 25), one-half (n = 35), and one-fifth (n = 55)] for 10 consecutive days. Concentrations of SPMA arising through induced benzene conjugation with glutathione were quantified by MS in sequential 12-h overnight urine collections during the intervention. RESULTS: MS was also used to quantify urinary sulforaphane metabolites in each dosing regimen that resulted in a median 24-h urinary output of 24.6, 10.3, and 4.3 µmol, respectively, confirming a dose-dependent de-escalation of the inducing principle within the beverage. A statistically significant increase in benzene mercapturic acids in urine was found for the high-dose group (+63.2%) during the 10-d period. The one-half dose (+11.3%) and one-fifth dose groups (-6.4%) were not significantly different from placebo controls. CONCLUSIONS: An intervention with a broccoli sprout beverage enhanced the detoxication of benzene, an important airborne pollutant, when dosed at a concentration evoking a urinary elimination of ∼25 µmol sulforaphane metabolites per day, and it portends a practical and frugal population-based strategy to attenuate associated long-term health risks of air pollution. This trial was registered at clinicaltrials.gov as NCT02656420.

11.
Drug Deliv ; 26(1): 393-403, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30929532

RESUMO

Our previous work indicates the lymphatic network and perivascular spaces or tissues might be involved in the facial intradermal brain-targeted delivery of Evans blue (EB). In this article, we presented the detailed involvement of both, and the linkage between lymphatic network and perivascular spaces or tissues. The in-vivo imaging, the trigeminal transection and immunohistochemistry were used. In-vivo imaging indicated intradermal injection in the mystacial pad (i.d.) delivered EB into the brain at 2-, 6- and 24 h, while intranasal injection (i.n.) delivered EB into the rostral head and intravenous injection (i.v.) diffused EB weakly into the brain. Trigeminal perineurial and epineurial EB occurred along the perivascular spaces or tissues and along brain vessels. EB diffused into the lymphatic vessels and submandibular lymph nodes. Moreover, perineurial and epineurial EB co-located or overlaid with Lyve1 immuno-reactivity and VEGF antibody, and lymphatic network connected with perivascular spaces or tissues, suggesting lymphatic system-perivascular spaces might involve in the EB delivery with i.d. The trigeminal transection reduced the trigeminal epineurial and perineurial EB and brain EB along vessels. EB diffused in the fasciculus and the perineurium, blood and lymphatic vessels in the mystacial pad, mystacial EB overlaid VEGF or Lyve1 antibody. In summary, the dermal-trigeminal-brain perivascular spaces or tissues and the linkage to the lymphatic network mediated the intradermal brain-targeted delivery.


Assuntos
Encéfalo/metabolismo , Corantes/administração & dosagem , Sistemas de Liberação de Medicamentos , Azul Evans/administração & dosagem , Administração Intranasal , Animais , Corantes/farmacocinética , Azul Evans/farmacocinética , Imuno-Histoquímica , Injeções Intradérmicas , Injeções Intravenosas , Linfonodos/metabolismo , Vasos Linfáticos/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos ICR , Ratos Sprague-Dawley , Fatores de Tempo , Distribuição Tecidual , Nervo Trigêmeo/metabolismo
12.
Int J Biol Sci ; 15(2): 430-440, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30745832

RESUMO

This study aimed to investigate the radiosensitizing effect of polydatin (PD) on colorectal cancer (CRC) and its underlying mechanism. The C57BL/6 mouse model of CRC was induced by treatment with azoxymethane (AOM)/dextran sodium sulfate (DSS) and then divided into four groups: control, PD alone, IR alone, and combination of PD and IR. Radiation therapy (200 cGy/min, 10Gy) was performed in mice in the experimental groups for once a week with a total of four times. Thirty minutes before IR, mice were intraperitoneally injected with PD at the dose of 25mg/kg. The number and volume of CRC xenografts were calculated. Immunohistochemical staining was performed to detect the expression of Ki67 and cleaved caspase-3 in tumor tissues samples. The effects of PD on proliferation and apoptosis were evaluated in CT26 and HCT116 colon tumor cells. Leucine-rich repeat-containing G-protein coupled receptor 5 positive (Lgr5+) cancer stem cells (CSCs) were sorted from CT26 cells and the effects of PD on their proliferation and apoptosis were observed to elucidate the radiosensitizing mechanism of PD in CRC cells. Combined therapy with PD and IR significantly decreased tumor volume, inhibited proliferation and induced apoptosis of tumor cells in the mouse model of CRC compared to other three groups. Compared to the IR group, in vitro assay showed that PD combined with IR inhibited proliferation and promoted apoptosis of CT26 and HCT116 colon tumor cells as well as Lgr5+ CSCs. However, addition of the bone morphogenetic protein (BMP) type I receptor inhibitor K02288 (6.4nM) dramatically increased proliferation of Lgr5+ CSCs and abolished the cytotoxic effect of PD combined with IR on Lgr5+ CSCs. The in vivo and in vitro experiments demonstrated that IR combined treatment with PD could inhibit proliferation and promote apoptosis of CRC cells and Lgr5+ CSCs, and BMP signaling pathway was involved in the radiosensitizing effect of PD.


Assuntos
Apoptose/efeitos dos fármacos , Neoplasias Colorretais/metabolismo , Glucosídeos/farmacologia , Estilbenos/farmacologia , Animais , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular , Células HCT116 , Humanos , Imuno-Histoquímica , Masculino , Camundongos , Camundongos Endogâmicos C57BL
13.
Food Funct ; 10(2): 635-645, 2019 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-30648709

RESUMO

The design of zein-based nanoparticles to encapsulate bioactive molecules has gained great attention in recent years. However, the use of ethanol to dissolve zein presents flammability concerns and the scale-up production of zein-based nanoparticles is also a concern. In our study, propolis loaded zein/caseinate/alginate nanoparticles were fabricated using a facile one-step procedure: a well-blended solution was prepared containing deprotonated propolis, soluble zein, dissociated sodium caseinate micelles (NaCas) and alginate at alkaline pH, and then this alkaline solution was added to 0.1 M citrate buffer (pH 3.8) to fabricate composite nanoparticles without using organic solvents and sophisticated equipment. During acidification, the alginate molecules adsorbed on the zein/NaCas surfaces by electrostatic complexation, which improved the stability towards aggregation of zein/NaCas nanoparticles under gastrointestinal (GI) or acidic pH. The nanoparticles prepared under the optimized method (method 3 sample) were of spherical morphology with a particle size around 208 nm and a negative zeta potential around -27 mV. The encapsulation efficiency (EE) and loading capacity (LC) of propolis reached 86.5% and 59.6 µg mg-1 by zein/NaCas/alginate nanoparticles, respectively. These nanoparticles were shown to be stable towards aggregation over a wide range of pH values (2-8) and salt concentrations (0-300 mM NaCl). Compared to free propolis, the bioaccessibility of propolis encapsulated with nanoparticles was increased to 80%. Our results showed a promising clean and scalability strategy to encapsulate hydrophobic nutraceuticals for applications in foods, supplements, and pharmaceuticals.


Assuntos
Alginatos/química , Caseínas/química , Nanopartículas/química , Própole/química , Zeína/química , Tecnologia de Alimentos , Microscopia Eletrônica de Transmissão
14.
Exp Gerontol ; 116: 93-105, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30590123

RESUMO

Due to dramatic growth of the aging population worldwide, there has been an urgent call for a public health strategy to manage healthy aging, with the ultimate goal being advancement of aging research. Considerable progress has been made in uncovering the mystery of aging process using multidisciplinary methods. There is a growing consensus in the field that aging traits which were originally thought to be disparate are likely to be interconnected. Thus, emerging research is needed to incorporate current findings of aging by building multiscale network models. This study reported the network of healthy aging research using bibliometric approaches. Based on the results, aging of the brain and muscle is a primary research focus which is a critical part of the multiscale network regulating the aging process. Among aging-associated diseases, Alzheimer's disease and frailty are among the main research focuses, and emerging work has focused on developing diagnostic tools for these diseases. For research on anti-aging interventions, calorie restriction, physical activity, and anti-aging pharmacology are the main interventions, of which the underlying mechanisms have been comprehensively studied in animal models.

15.
Theranostics ; 8(19): 5482-5500, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30555559

RESUMO

Background: Treatment of large bone defects represents a major clinical problem worldwide. Suitable bone substitute materials are commonly required to achieve successful bone regeneration, and much effort has been spent to optimize their chemical compositions, 3D architecture and mechanical properties. However, material-immune system interactions are increasingly being recognized as a crucial factor influencing regeneration. Here, we envisioned an accurate and proactive immunomodulation strategy via delivery of IL-4 (key regulator of macrophage polarization) to promote bone substitute material-mediated regeneration. Methods: Four different IL-4 doses (0 ng, 10 ng, 50 ng and 100 ng) were delivered into rat large cranial bone defects at day 3 post-operation of decellularized bone matrix (DBM) material implantation, and the osteogenesis, angiogenesis and macrophage polarization were meticulously evaluated. Results: Micro-CT analysis showed that immunomodulation with 10 ng IL-4 significantly outperformed the other groups in terms of new bone formation (1.23-5.05 fold) and vascularization (1.29-6.08 fold), achieving successful defect bridging and good vascularization at 12 weeks. Histological analysis at 7 and 14 days showed that the 10 ng group generated the most preferable M1/M2 macrophage polarization profile, resulting in a pro-healing microenvironment with more IL-10 and less TNF-α secretion, a reduced apoptosis level in tissues around the materials, and enhanced mesenchymal stem cell migration and osteogenic differentiation. Moreover, in vitro studies revealed that M1 macrophages facilitated mesenchymal stem cell migration, while M2 macrophages significantly increased cell survival, proliferation and osteogenic differentiation, explaining the in vivo findings. Conclusions: Accurate immunomodulation via IL4 delivery significantly enhanced DBM-mediated osteogenesis and angiogenesis via the coordinated involvement of M1 and M2 macrophages, revealing the promise of this accurate and proactive immunomodulatory strategy for developing new bone substitute materials.


Assuntos
Doenças Ósseas/terapia , Substitutos Ósseos/administração & dosagem , Fatores Imunológicos/administração & dosagem , Interleucina-4/administração & dosagem , Osteogênese , Animais , Substitutos Ósseos/metabolismo , Movimento Celular , Citocinas/análise , Modelos Animais de Doenças , Imuno-Histoquímica , Fatores Imunológicos/metabolismo , Interleucina-4/metabolismo , Macrófagos/imunologia , Células-Tronco Mesenquimais/fisiologia , Neovascularização Fisiológica , Ratos , Regeneração , Crânio/patologia , Resultado do Tratamento , Microtomografia por Raio-X
16.
Anal Chem ; 90(24): 14230-14238, 2018 12 18.
Artigo em Inglês | MEDLINE | ID: mdl-30398847

RESUMO

Recent outbreaks of Ebola-virus infections in several countries demand a rapid point-of-care (POC)-detection strategy. This paper reports on an innovative pathway founded on electronic-resonance-frequency modulation to detect Ebola glycoprotein (GP), on the basis of a carrier-injection-trapping-release-transfer mechanism and the standard antibody-antigen-interaction principle within a dielectric-gated reduced graphene oxide (rGO) field-effect transistor (GFET). The sensitivity of Ebola detection can be significantly enhanced by monitoring the device's electronic-resonance frequency, such as its inflection frequency ( fi), where the phase angle reaches a maximum (θmax). In addition to excellent selectivity, a sensitivity of ∼36-160% and ∼17-40% for 0.001-3.401 mg/L Ebola GP can be achieved at high and low inflection-resonance frequencies, respectively, which are several orders of magnitude higher than the sensitivity from other electronic parameters (e.g., resistance-based sensitivity). Using equivalent circuit modeling for contributions from channel and contact, analytical equations for resonance shifts have been generalized. When matching with the incoming ac-measurement signal, electronic resonance from the phase-angle spectrum evolves from various relaxation processes (e.g., trap and release of injected charges at surface-trap sites of the channel-gate oxide and channel-source or drain interfaces) that are associated with a characteristic emission frequency. Using charge-relaxation dynamics, a high-performance bio-FET sensing platform for healthcare and bioelectronic applications is realized through resonance shifting.


Assuntos
Ebolavirus/metabolismo , Grafite/química , Sistemas Automatizados de Assistência Junto ao Leito , Transistores Eletrônicos , Proteínas Virais/imunologia , Anticorpos Imobilizados/química , Anticorpos Imobilizados/imunologia , Anticorpos Antivirais/química , Anticorpos Antivirais/imunologia , Reações Antígeno-Anticorpo , Ouro/química , Doença pelo Vírus Ebola/diagnóstico , Humanos , Nanopartículas Metálicas/química , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/imunologia , Proteínas Recombinantes/isolamento & purificação , Ressonância de Plasmônio de Superfície , Proteínas Virais/genética , Proteínas Virais/metabolismo
18.
Nurse Educ Today ; 71: 121-128, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30286369

RESUMO

BACKGROUND: Simulation is recommended as a substitute for clinical practice among nursing students. No current guidelines exist regarding the accurate percentage of simulation hours versus clinical practice hours. Comparing simulation with clinical practice is needed so that both strategies can be optimally combined in nursing education. The 29-item Clinical Learning Environment Comparison Survey (CLECS) is validated to compare the traditional and simulated clinical environment in meeting nursing students' learning needs. This type of tool is not available in China. OBJECTIVES: This study aimed to translate and test the psychometric properties of CLECS for Chinese undergraduate nursing students. DESIGN: This is a cross-sectional study. SETTINGS: Two nursing schools in Central and East China. PARTICIPANTS: A total of 179 undergraduate nursing students who had participated in both traditional and high fidelity simulated clinical practice were recruited. METHODS: A standard procedure with forward translation, back translation, cultural adaptation and pilot testing was followed to test the CLECS (Chinese version). An exploratory factor analysis was used to establish a modified factor structure of CLECS (Chinese version); a confirmatory factor analysis verified its construct validity. Reliability of the CLECS (Chinese version) was estimated using the intraclass correlation coefficients (ICCs) and Cronbach's alpha coefficients. RESULTS: The exploratory factor analyses explained 61.43% and 60.11% of the total variances in traditional and simulated clinical environment. The proposed factor solution of the CLECS (Chinese version) obtained satisfactory model fit and nesting model between two nursing schools. In the proposed model, ICCs were 0.61 and 0.93, and Cronbach's alpha coefficients were 0.75 and 0.95 in the traditional and simulated clinical environment. CONCLUSIONS: The CLECS (Chinese version) showed satisfactory reliability and validity among Chinese undergraduate nursing students. Further validation of the CLECS (Chinese version) is needed in a more representative and larger sample. The CLECS (Chinese version) should be further tested as an effective tool to compare the traditional and simulated clinical practice among Chinese nursing schools.


Assuntos
Competência Clínica/normas , Avaliação Educacional/normas , Psicometria/normas , Estudantes de Enfermagem/estatística & dados numéricos , China , Estudos Transversais , Bacharelado em Enfermagem/métodos , Bacharelado em Enfermagem/normas , Avaliação Educacional/métodos , Feminino , Humanos , Masculino , Psicometria/instrumentação , Psicometria/métodos , Reprodutibilidade dos Testes , Estudantes de Enfermagem/psicologia , Inquéritos e Questionários , Tradução , Adulto Jovem
19.
Chin J Integr Med ; 2018 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-30328567

RESUMO

OBJECTIVE: To investigate the effects of Composition of Ophiopogon polysaccharide, Notoginseng total saponins and Rhizoma Coptidis alkaloids (CONR) on myocardial apoptosis of diabetic atherosclerosis (DA) rabbits METHODS: Sixty male New Zealand white rabbits were randomly divided into 6 groups [control group, model group, CONR high-dose group (450 mg/kg), CONR medium-dose group (150 mg/kg), CONR low-dose group (50 mg/kg), and simvastatin group] by using a completely random method, 10 in each group. DA model was established by intravenously injected alloxan combined with high-fat diet and abdominal aortic balloon injury. After mediation for 10 weeks, fasting blood glucose (FBG), glycosylated hemoglobin (GHB), glycosylated serum protein (GSP), fructoseamine (FRA), aldose reductase (AR), advanced glycation end products (AGEs) in serum were measured by enzyme linked immunosorbent assay (ELISA) method; the expression of receptor of AGEs (RAGE) in myocardial tissue were observed by immunohistochemical method; and p-Jun N-terminal kinase (p-JNK), caspase-3, B-cell lymphoma-2 (bcl-2) protein expression in myocardial tissue were measured by Western blotting. The myocardial apoptosis was detected by TdT-mediated dUTPnick-end labeling (TUNEL) method, and apoptosis index (AI) was calculated. RESULTS: Compared with the control group, serum FBG, GHB, GSP, FRA, AR, AGEs and the expression of myocardium RAGE, p-JNK, caspase-3 proteins, as well as apoptosis index (AI) were significantly increased and bcl-2 protein was significantly decreased in the model group (P<0.01). Compared with the model group, the levels of serum FBG, GHB, GSP, FRA and AR showed a significant decline in CONR high- and medium-dose groups (P<0.01). FBG and GHB showed a significant decline in CONR low-dose group (P<0.01). Compared with the model group, the expression of serum AGEs and myocardium RAGE, p-JNK and caspase-3 protein as well as AI were significantly decreased and bcl-2 protein was significantly up-regulated in all treatment groups (P<0.01); high-dose CONR had the most significant effect on abovementioned indices compared with other treatment groups (P<0.01). Middle-dose CONR had better effect on serum AGEs compared with the low-dose group (P<0.01); middle-dose CONR and simvastatin groups had better effect on the expression of caspase-3, bcl-2 protein, myocardium apoptosis compared with the CONR low-dose group (P<0.01). CONCLUSION: CONR may effectively inhibit myocardial apoptosis on DA rabbits by intervening AGEs-RAGE and JNK, caspase-3, and bcl-2 protein expressions.

20.
Analyst ; 143(22): 5583-5588, 2018 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-30318531

RESUMO

The development of fluorescent probes to selectively and sensitively detect more than one target analyte under a given condition is a challenging task due to the lack of designing strategies. In this paper, we report the utilisation of the solvatochromic properties of a coumarin-pyridine based probe L1 to detect Mg2+ and PPi, respectively, by using different solvents such as acetonitrile, DMF and DMF-HEPES buffered solutions. When acetonitrile was used as the solvent, L1 was weakly fluorescent and showed a selective and sensitive fluorescence "turn on" response towards Mg2+ with a detection limit of 86 nM, while in DMF and DMF-HEPES buffered solutions, L1 was brightly fluorescent and was able to form a non-fluorescent L1-Fe3+ complex. The obtained L1-Fe3+ complex was further determined to be a selective and sensitive fluorescent "turn on" probe for PPi with a detection limit of 94 nM.

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